Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
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| use of a pre-selected epitope of cathepsin-l1 in a highly specific peptide-based immunoassay for the diagnosis of fasciola hepatica infections in cattle. | a peptide-based indirect elisa to detect cattle antibodies against fasciola hepatica was developed and evaluated for its sensitivity and specificity. an immunogenic antigen released in vitro by f. hepatica was purified. after purification the sequence of the first 20 n-terminal aa of this protein showed considerable homology with cathepsin l-like proteinase. based on its homology with cathepsin-l1, we further focused on this protein for diagnostic purpose. predicted b-cell epitopes of cathepsin- ... | 1999 | 10404262 |
| alternative-splicing of serotonin receptor isoforms in the pharynx and muscle of the parasitic nematode, ascaris suum. | pharyngeal pumping is essential for nematode feeding and survival and is dramatically stimulated by serotonin (5-ht). in the present study, a cdna pool was prepared from poly a + rna isolated from pharynxes dissected from the parasitic nematode, ascaris suum, and was used as a template for rt-pcr with degenerate primers designed from sequences conserved in 5-ht receptors from a variety of sources. a putative 5-ht receptor cdna (as1) was identified which exhibited most identity to the 5-ht2 famil ... | 1999 | 10413046 |
| toxocariasis: serological diagnosis by indirect antibody competition elisa. | toxocariasis is caused by infection of man by toxocara canis and toxocara cati larvae, the common roundworm of dogs and cats. because larvae are difficult to detect in tissues, diagnosis is mostly based on serology. non specific reactions are observed mainly due to cross-reactivity with ascaris sp antigens. this investigation aimed at developing and evaluating an indirect antibody competition elisa (iace) employing a specific rabbit igg anti-toxocara canis excretory-secretory antigens as the com ... | 1999 | 10413956 |
| mapping the active site topography of the nad-malic enzyme via alanine-scanning site-directed mutagenesis. | the nad-malic enzyme cdna has been subcloned into the pqe expression vector, expressed with a six-his tag, and purified. the his-tagged enzyme is purified by a combination of ni-nta and orange a agarose column chromatography with a yield of 45% and an estimated purity of >90%. the tag and linker have no effect on the kinetic parameters of the enzyme compared to the wild-type enzyme. alanine-scanning site-directed mutagenesis has been carried out on all of the conserved neutral acid residues of t ... | 1999 | 10441149 |
| preparation of recombinant rat interleukin-5 by baculovirus expression system and analysis of its biological activities. | rat interleukin-5 (il-5) cdna was subcloned from peritoneal cells collected 4 h after intraperitoneal injection of ascaris suum antigen solution into the immunized rats. cysteine proteinase-deleted (cpd) rat il-5 recombinant virus was constructed by inserting rat il-5 cdna into cpd virus having a deletion in the cysteine proteinase gene of the silkworm bombyx mori nuclear polyhedrosis virus. on infection with the cpd rat il-5 recombinant virus, the silkworm b. mori larvae produced rat il-5 as a ... | 1999 | 10446387 |
| ascaris suum: seasonal egg development rates in a saskatchewan pig barn. | this study investigated the effects of seasonal temperature variations on the rate of development to infectivity of ascaris suum eggs under western canadian climatic conditions. flasks containing a suspension of a. suum eggs were placed inside a pig barn located near saskatoon, saskatchewan, canada, at monthly intervals from july 1997 to july 1998. eggs from each flask were monitored weekly for development to the infective larval stage. infectivity of eggs was confirmed using a mouse bioassay. d ... | 1999 | 10447194 |
| the morphogenesis of ascaris suum to the infective third-stage larvae within the egg. | studies of the morphology of ascaris suum larvae developing in the egg during embryonation in vitro at room temperature showed that 2 molts take place within the egg. the first larval stage (l1) appeared in the egg after 17-22 days of cultivation, the first molt to the second larval stage (l2) took place from day 22 to day 27, and the second molt to the third larval stage (l3) started on day 27 and continued during the 60-day observation period. infectivity of the eggs was studied by oral egg in ... | 1999 | 10461940 |
| seasonal variation in development and survival of ascaris suum and trichuris suis eggs on pastures. | pig faeces were deposited on experimental plots in the spring, summer, autumn and winter to study development and survival of ascaris suum and trichuris suis eggs under outdoor conditions. faeces were placed either in short grass or 2 cm below the surface of bare soil, imitating pastures used by nose-ringed, grazing pigs or normally rooting pigs, respectively. the numbers and developmental stages of the eggs were recorded in faeces and soil for up to 50 weeks post-deposition. embryonation took p ... | 1999 | 10466129 |
| hepatic imaging studies on patients with visceral larva migrans due to probable ascaris suum infection. | visceral larva migrans (vlm) is a disease usually observed in children in which the larvae of animal parasites invade and reside in human tissues for long periods. although the common causal species of vlm are toxocara canis and t. cati, we identified three adult patients with vlm, probably due to ascaris suum, whose diagnosis was made by specific immunoserological tests. the patients complained of respiratory symptoms, and laboratory tests showed pronounced eosinophilia, but neither larvae nor ... | 1999 | 10475929 |
| expression and characterization of elastase inhibitors from the ascarid nematodes anisakis simplex and ascaris suum. | two elastase inhibitors, aspi-1 and aspi-2, from the parasitic nematode anisakis simplex, have been isolated and characterized. because these inhibitors are similar in size (60 amino acids in length) and primary sequence (52 and 47% identical) to the ascaris suum chymotrypsin/elastase inhibitor-1 (asc/e-1), we suggest that these anisakis elastase inhibitors belong to the same unique class of canonical inhibitors formed by the family of ascaris inhibitors (huang k, strynadka ncj, bernard vd, pean ... | 1999 | 10477178 |
| localized depolymerization of the major sperm protein cytoskeleton correlates with the forward movement of the cell body in the amoeboid movement of nematode sperm. | the major sperm protein (msp)-based amoeboid motility of ascaris suum sperm requires coordinated lamellipodial protrusion and cell body retraction. in these cells, protrusion and retraction are tightly coupled to the assembly and disassembly of the cytoskeleton at opposite ends of the lamellipodium. although polymerization along the leading edge appears to drive protrusion, the behavior of sperm tethered to the substrate showed that an additional force is required to pull the cell body forward. ... | 1999 | 10477761 |
| health status of a recently discovered population of feral swine in kansas. | twenty feral hogs (sus scrofa) from a newly discovered population on fort riley army base (kansas, usa) were shot and examined from november 1993 through february 1994 to assess the health of the population. the hogs were generally healthy, although serologic evidence indicated that some individuals had been exposed to parvovirus, enterovirus, and swine influenza. we found no indications of brucellosis, pseudorabies, or porcine respiratory and reproductive syndrome. lung worms (metastrongylus sp ... | 1999 | 10479106 |
| cutting edge: mouse igg1 antibodies comprise two functionally distinct types that are differentially regulated by il-4 and il-12. | il-4-dependent and -independent igg1 abs differ in their ability to induce mast cell degranulation as measured by passive cutaneous anaphylaxis (pca). mice immunized with ova or piii (fraction of ascaris suum) produced high titers of igg1 as shown by elisa and pca. in contrast, another a. suum fraction, pi, elicited igg1 abs with no pca activity. igg1 with anaphylactic activity required il-4, as igg1 responses to ova and piii in il-4-/- mice gave no pca. pi-specific igg1 was il-4-independent, be ... | 1999 | 10490948 |
| defined characteristics of cathepsin b-like proteins from nematodes: inferred functional diversity and phylogenetic relationships. | numerous cathepsin b-like protein sequences (cbls) have been reported from nematodes. however, the relationships among these proteins remain unclear. here, expression of several cbl transcripts in the gut of the parasitic nematode haemonchus contortus was demonstrated. to assess potential functional diversity, multiple nematode cbl sequences were compared with known functional domains of cathepsin b. these domains included the occluding loop, s2' and s2 subsites, and the pro region. four groups ... | 1999 | 10498185 |
| the haemoglobin enzyme. | 1999 | 10519542 | |
| 1h nmr investigation of the distal hydrogen bonding network and ligand tilt in the cyanomet complex of oxygen-avid ascaris suum hemoglobin. | the o(2)-avid hemoglobin from the parasitic nematode ascaris suum exhibits one of the slowest known o(2) off rates. solution (1)h nmr has been used to investigate the electronic and molecular structural properties of the active site for the cyano-met derivative of the recombinant first domain of this protein. assignment of the heme, axial his, and majority of the residues in contact with the heme reveals a molecular structure that is the same as reported in the a. suum hbo(2) crystal structure ( ... | 1999 | 10542205 |
| free-living nematodes caenorhabditis elegans possess in their mitochondria an additional rhodoquinone, an essential component of the eukaryotic fumarate reductase system. | the respiratory chain of caenorhabditis elegans was characterized in mitochondria isolated from aerobically grown nematodes. nematode mitochondria contain ubiquinone-9 as a major component and rhodoquinone-9 as a minor component. the ratio of ubiquinone-9/rhodoquinone-9 is higher in c. elegans mitochondria than in mitochondria from second-stage larvae of ascaris suum, the free-living stage of porcine gut-dwelling nematode. the individual oxidoreductase activities comprising succinate oxidase and ... | 1999 | 10545216 |
| ligand-gated chloride channel subunits encoded by the haemonchus contortus and ascaris suum orthologues of the caenorhabditis elegans gbr-2 (avr-14) gene. | the alternatively-spliced caenorhabditis elegans gbr-2/avr-14 gene encodes two subunits of the nematode ligand-gated chloride channel family which forms an important molecular target for the avermectin and related anthelminthics. we used reverse transcriptase-polymerase chain reaction (rt-pcr) techniques to isolate cdnas encoding the products of the gbr-2/avr-14 orthologues from the parasitic nematodes haemonchus contortus and ascaris suum. the predicted polypeptides possess all the characterist ... | 1999 | 10551358 |
| effect of the injection of an extract of ascaris suum on macrophage activation during the early phase of mycobacterium bovis bcg infection in c57bl/6 mice. | injection of an ascaris suum extract (asc) affects both the humoral and cellular immune responses to unrelated antigens when it is co-administered with these antigens. in the present study we evaluated the effect of asc on macrophage activation in the early phase of mycobacterium bovis bcg (pasteur strain tmcc 1173) infection in c57bl/6 mice. c57bl/6 mice were injected intraperitoneally (ip) with 0.1 mg bcg (bcg group) or bcg plus 1 mg asc (bcg + asc group). the peritoneal exudates were obtained ... | 1999 | 10559845 |
| neuromusculature of the ovijector of ascaris suum (ascaroidea, nematoda): an ultrastructural and immunocytochemical study. | this study used electron microscopy and confocal scanning laser microscopy interfaced with cytochemistry to study neuromuscular interrelationships in the ovijector of ascaris suum. an extensive nerve plexus with both farpergic and non-farpergic components extends over the outer surface of the ovijector. the non-farpergic component is derived from nerve branches of the ventral nerve cord, whereas the farpergic component emanates from two large fmrfamide-immunoreactive neurons. in the vagina vera, ... | 1999 | 10570459 |
| isolation, characterization and immunolocalization of phosphorylcholine-substituted glycolipids in developmental stages of caenorhabditis elegans. | caenorhabditis elegans displays three neutral glycosphingolipids with structural homology to glycosphingolipids from the porcine nematode parasite, ascaris suum. the present findings extend the degree of structural conservation between the two nematode species to glycosphingolipids with a phosphodiester substitution. using a combination of hydrofluoric acid pretreatment, immunochemical characterization and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, three zwitte ... | 1999 | 10583390 |
| experimental ascaris suum infection in the pig: protective memory response after three immunizations and effect of intestinal adult worm population. | the protective immune response to larval migration in pigs, with or without adult intestinal worm populations, 10 weeks after 3 weekly ascaris suum inoculations, was studied in 45 pigs. controlled adult worm populations were achieved by oral transfer of 10 adult worms to previously immunized pigs after anthelmintic drenching. a significant reduction in larval recovery from lungs on day 7, and small intestine on day 14, was observed in immunized pigs compared with previously uninfected control pi ... | 1999 | 10583864 |
| eotaxin and nitric oxide production as markers of inflammation in allergic cynomolgus monkeys. | cynomolgus monkeys have a natural hypersensitivity to ascaris suum antigen. inhalation of antigen produces immediate and delayed allergic reactions and an influx of inflammatory cells into the lungs. this study investigated the production of nitric oxide (no) and the chemokine eotaxin during this allergic response. the effect of bronchoscopy alone on lung inflammatory cells was also investigated along with the time course of the eosinophil influx into the lung. | 1999 | 10592466 |
| development of patent ascaris suum infections in pigs following intravenous administration of larvae hatched in vitro. | the normal tissue migration of ascaris suum in the pig host involves larval development in the liver accompanied by considerable pathological changes. the vast majority of larvae that reach the small intestine are later expelled by unknown mechanisms. we show that when migration through the liver is bypassed by inoculation of pigs with an intravenous dose of larvae hatched in vitro, the larvae not only complete migration and return to the small intestine, but they also seem to have a greater cha ... | 1999 | 10599082 |
| exposure of sows to ascaris suum influences worm burden distributions in experimentally infected suckling piglets. | this paper reports on the influence of maternal exposure to ascaris suum on worm burden distributions in experimentally infected piglets. in the first study, sows were inoculated before and during gestation (6 months, long-term exposure) with 10,000 a. suum eggs twice weekly. in a second study, sows were inoculated during gestation only (3 months, short-term exposure) with increasing doses of eggs (10,000-40,000 eggs twice weekly). helminth-naive sows served as controls in both studies. the thir ... | 1999 | 10599083 |
| intestinal parasites in swine in the nordic countries: multilevel modelling of ascaris suum infections in relation to production factors. | in denmark, finland, iceland, norway and sweden, 413 sow herds were randomly selected for sampling. faeces from pigs of 7 age groups/categories were examined for helminth eggs (11,233 individual samples), and an accompanying questionnaire was completed at each visit. in total, 1138 pigs on 230 farms were found to be positive for ascaris suum. considerable differences in the occurrence of a. suum could be observed directly for several of 20 independent variables at the herd or category level. how ... | 1999 | 10599084 |
| establishment of ascaris suum in the pig: development of immunity following a single primary infection. | development of immunity after a single primary infection of ascaris suum in pigs was investigated with regard to the worm population dynamics of a superimposed a. suum infection, host immune response and gross liver pathological changes. group a was given a primary infection of 60,000 infective a. suum eggs and group b was left uninfected. four weeks later both groups a and b were inoculated with 1,000 a. suum eggs, and subgroups were slaughtered 7, 14 and 21 days post challenge infection (p.c.i ... | 1999 | 10605128 |
| structural elucidation and monokine-inducing activity of two biologically active zwitterionic glycosphingolipids derived from the porcine parasitic nematode ascaris suum. | the isolated neutral glycosphingolipid fraction from the pig parasitic nematode, ascaris suum, was fractionated by silica gel chromatography to yield a neutral and a zwitterionic glycosphingolipid fraction, the latter of which mainly contained two zwitterionic glycosphingolipids termed components a and c. preliminary chemical characterization with hydrofluoric acid treatment and immunochemical characterization with a phosphocholine-specific monoclonal antibody indicated that both components cont ... | 1998 | 9417105 |
| hydrostatic pressure shows that lamellipodial motility in ascaris sperm requires membrane-associated major sperm protein filament nucleation and elongation. | sperm from nematodes use a major sperm protein (msp) cytoskeleton in place of an actin cytoskeleton to drive their ameboid locomotion. motility is coupled to the assembly of msp fibers near the leading edge of the pseudopod plasma membrane. this unique motility system has been reconstituted in vitro in cell-free extracts of sperm from ascaris suum: inside-out vesicles derived from the plasma membrane trigger assembly of meshworks of msp filaments, called fibers, that push the vesicle forward as ... | 1998 | 9442112 |
| a novel uv-damaged dna binding protein emerges during the chromatin-eliminating cleavage period in ascaris suum. | during the early cleavage period of ascaris suum , chromatin diminution takes place in the somatic founder cells. in the process of chromatin diminution numerous heterochromatic blocks, consisting predominantly of highly repeated dna, are discarded during mitotic anaphase and are later on digested in the cytoplasm. very little is known about proteins that are involved in chromatin diminution. we have detected a nuclear protein and purified it to near homogeneity by its preferential binding to uv ... | 1998 | 9443969 |
| inhibition of allergic airway responses by inhaled low-molecular-weight heparins: molecular-weight dependence. | inhaled heparin prevents antigen-induced bronchoconstriction and inhibits anti-immunoglobulin e-mediated mast cell degranulation. we hypothesized that the antiallergic action of heparin may be molecular weight dependent. therefore, we studied the effects of three different low-molecular-weight fractions of heparin [medium-, low-, and ultralow-molecular-weight heparin (mmwh, lmwh, ulmwh, respectively)] on the antigen-induced acute bronchoconstrictor response (abr) and airway hyperresponsiveness ( ... | 1998 | 9451639 |
| immunomodulation induced by ascaris suum extract in mice: effect of anti-interleukin-4 and anti-interleukin-10 antibodies. | simultaneous immunization of mice with an ascaris suum extract (asc) and ovalbumin (oa) markedly affects the immune response to oa. the role of interleukin (il)-4 and il-10 induced by asc immunization on the modulation of antigen-specific and mitogen-induced responses was investigated following single or combined cytokine-specific monoclonal antibody (moab) treatment of mice before immunization with oa + asc. immediate hypersensitivity reactions to aggregated oa and oa-specific immunoglobulin (i ... | 1998 | 9467652 |
| release of epithelium-derived pge2 from canine trachea after antigen inhalation. | to investigate the role of prostaglandin (pg) e2 in allergen-induced hyperresponsiveness, dogs inhaled either the allergen ascaris suum or vehicle (sham). twenty-four hours after inhalation, some animals exposed to allergen demonstrated an increased responsiveness to acetylcholine challenge in vivo (hyp-resp), whereas others did not (non-resp). strips of tracheal smooth muscle, either epithelium intact or epithelium denuded, were suspended on stimulating electrodes, and a concentration-response ... | 1998 | 9486206 |
| early ascaris suum migration in mice as a model for pigs. | a study was made of the early migratory pattern of ascaris suum in mice. mice were each inoculated orally with a single dose of 2,500 infective eggs and then killed at 2, 4, 6, 8, 10, and 12 hr postinoculation (pi). at necropsy, it was observed that the larvae had penetrated the mouse cecum and colon and had reached the liver by 4 hr pi. this migratory behavior closely mimics what is observed in the pig and suggests that the mouse may serve as an experimental model for intestinal immunity in the ... | 1998 | 9488331 |
| developmental and tissue-specific expression of 2-methyl branched-chain enoyl coa reductase isoforms in the parasitic nematode, ascaris suum. | the 2-methyl branched-chain enoyl coa reductase (ecr) plays a pivotal role in the reversal of beta-oxidation operating in anaerobic mitochondria of the parasitic nematode, ascaris suum. two-dimensional gel electrophoresis of the purified ecr yielded multiple spots, with two distinct but overlapping n-terminal sequences. these multiple isoforms were not the result of population effects, as the pattern observed on 2-d gels of the purified ecr was identical to those on immunoblots of muscle homogen ... | 1998 | 9566523 |
| growth and structural features of the adult stage of ascaris suum (nematoda, ascaridoidea) from experimentally infected domestic pigs. | for studying the morphogenesis of the adult stage of ascaris suum, worms were obtained from experimentally infected domestic pigs. structural and morphometric features of a. suum are analyzed by light and scanning electron microscopy. it was shown that the increase in length of the worm from days 35 to 56 is based on a region-specific lengthening of individual transverse annuli in the cuticle. an increased worm size also results in an increased diameter of different cuticular sense organs. it is ... | 1998 | 9576498 |
| molecular cloning, functional expression, and characterization of pyruvate dehydrogenase kinase from anaerobic muscle of the parasitic nematode ascaris suum. | the pyruvate dehydrogenase complex (pdc) plays a key role in the anaerobic mitochondrial metabolism of the parasitic nematode ascaris suum. a cdna coding for an a. suum pyruvate dehydrogenase kinase (apdk) has been cloned and sequenced from poly(a)+ rna isolated from adult a. suum muscle.2 apdk exhibited significant sequence identity to mammalian pdks. nucleotide sequence analysis of the apdk cdna revealed a 22-nucleotide spliced leader, characteristic of many nematode mrnas, a 5'-utr of 6 nucle ... | 1998 | 9578613 |
| expression of pyruvate dehydrogenase isoforms during the aerobic/anaerobic transition in the development of the parasitic nematode ascaris suum: altered stoichiometry of phosphorylation/inactivation. | the pyruvate dehydrogenase complex (pdc) plays a key role in the anaerobic metabolism of the parasitic nematode ascaris suum. two isoforms of the alpha-subunit of pyruvate dehydrogenase (e1) have been identified: alpha i is most abundant in anaerobic adult muscle and alpha ii in aerobic larvae. both isoforms have been expressed as alpha 2 beta 2 tetramers with a muscle-specific beta-subunit, purified to apparent homogeneity, reconstituted with e1-deficient adult a. suum muscle pdc, and assayed f ... | 1998 | 9587415 |
| an in-vitro test for assessing the viability of ascaris suum eggs exposed to various sewage treatment processes. | a simple procedure using ascaris suum as a model for ascaris lumbricoides in testing the ability of sludge treatment processes to kill the eggs of parasitic roundworms was developed. unembryonated and embryonated a. suum eggs were placed in a mesophilic anaerobic sludge digester or a sludge lagoon, or stored at 4 degrees c. unembryonated eggs were recovered and incubated at 32.5 degree c and 90-95% r.h. for 48 h and viable eggs developed embryos consisting of two or more clearly defined cells. t ... | 1998 | 9602387 |
| neurophysiology of glutamatergic signalling and anthelmintic action in ascaris suum: pharmacological evidence for a kainate receptor. | electrophysiological and pharmacological techniques were used to study glutamatergic signalling in the parasitic nematode, ascaris suum. glutamate or kainate injections into whole worms produced a paralysed quasi-static posture similar to the waveform in behaving worms. the de2 motorneuron class is a primary target. several glutamatergic substances produced pronounced conductance increases and depolarization in de2; domoate and kainate were the most potent agonists tested. glutamate responses an ... | 1998 | 9614330 |
| action of excitatory amino acids on hypodermis and the motornervous system of ascaris suum: pharmacological evidence for a glutamate transporter. | electrophysiological and pharmacological experiments suggest the presence of an electrogenic glutamate transporter in the motornervous system of the parasitic nematode ascaris suum. this putative transporter occurs in hypodermis (a tissue in some respects analogous to glia) and in de2 motorneurons, a dorsal excitatory motorneuron class which receives excitatory glutamatergic post-synaptic potentials. glutamate application to hypodermis produced non-conductance mediated depolarizations that were ... | 1998 | 9614331 |
| [a case of pulmonary infiltration with eosinophilia caused by ascaris suum]. | a 46-year old man was admitted to our hospital with dry cough and diarrhea. his chest ct showed diffuse subpleural small nodular shadows. eosinophilia and an increase of serum ige were detected. eosinophils were also increased in balf. an antibody against ascaris suum was positive in serum while other causes of eosinophilia (e. g., drugs, fungus, collagen disease) were absent. ivermectin was administered and the diarrhea improved within 3 days. the abnormal chest shadows and dry cough disappeare ... | 1998 | 9617152 |
| allergen challenge increases cell traffic between bone marrow and lung. | increases in inflammatory-cell progenitors have been demonstrated in the bone marrow (bm) after inhalation of ascaris suum in dogs at the time of allergen-induced airway hyperresponsiveness (ahr). the aim of this study was to evaluate the effect of allergen challenge on trafficking of inflammatory cells and their progenitors from the bm to the lung, using a marker of proliferating cells, bromodeoxyuridine (brdu). brdu is a thymidine analogue taken up by the dna of dividing cells, and can be dete ... | 1998 | 9618380 |
| detection of sequence variation in parasite ribosomal dna by electrophoresis in agarose gels supplemented with a dna-intercalating agent. | this study evaluated the use of a commercially available dna intercalating agent (resolver gold) in agarose gels for the direct detection of sequence variation in ribosomal dna (rdna). this agent binds preferentially to at sequence motifs in dna. regions of nuclear rdna, known to provide genetic markers for the identification of species of parasitic ascarid nematodes (order ascaridida), were amplified by polymerase chain reaction (pcr) and subjected to electrophoresis in standard agarose gels ve ... | 1998 | 9629896 |
| anisakis simplex: mutational bursts in the reactive site centers of serine protease inhibitors from an ascarid nematode. | 1998 | 9635450 | |
| embryonation and infectivity of ascaris suum eggs isolated from worms expelled by pigs treated with albendazole , pyrantel pamoate, ivermectin or piperazine dihydrochloride. | the effect of anthelmintic treatment of pigs on the embryonation and infectivity of ascaris suum eggs isolated from expelled worms was investigated. four groups of two naturally infected pigs were dosed with albendazole, pyrantel pamoate, ivermectin or piperazine dihydrochloride, respectively. following worm expulsion, the eggs were removed from the uteri of female worms and embryonated in sulphuric acid. the infectivity of the embryonated eggs was tested through mouse inoculation. egg developme ... | 1998 | 9637219 |
| studies on the survival of ascaris suum eggs under laboratory and simulated field conditions. | a series of four experiments was carried out to study the survival of ascaris suum eggs: in a pig slurry unit on a farm, in the laboratory under anaerobic conditions and different relative humidities (rh), and under simulated field conditions. survival of eggs in the pig slurry unit was 20% after four weeks and 0% after 16 weeks. anaerobic conditions had only a minor influence on survival: after 12 weeks, more than 80% of the eggs could still develop. at high relative humidities (100% and 75%), ... | 1998 | 9637224 |
| identification of protein-protein interactions of the major sperm protein (msp) of caenorhabditis elegans. | in nematodes, sperm are amoeboid cells that crawl via an extended pseudopod. unlike those in other crawling cells, this pseudopod contains little or no actin; instead, it utilizes the major sperm protein (msp). in vivo and in vitro studies of ascaris suum msp have demonstrated that motility occurs via the regulated assembly and disassembly of msp filaments. filaments composed of msp dimers are thought to provide the motive force. we have employed the yeast two-hybrid system to investigate msp-ms ... | 1998 | 9641981 |
| stable isotope-edited nmr analysis of ascaris suum mitochondrial trnamet having a tv-replacement loop. | most nematode mitochondrial (mt) trnas have a tv-replacement loop (tv loop) which replaces the normal t arm and the variable loop in standard trnas with a less-structured loop. the tertiary structure of such trnas has been discussed theoretically with reference to the crystal structure of yeast trnaphe [wolstenholme et al. (1994) nucleic acids res. 22, 4300-4306] and examined experimentally by chemical and enzymatic probing [watanabe et al. (1994) j. biol. chem. 269, 22902-22906]. the results su ... | 1998 | 9644241 |
| intestinal parasites in swine in the nordic countries: prevalence and geographical distribution. | in denmark (dk), finland (fin), iceland (i), norway (n), and sweden (s), 516 swine herds were randomly selected in 1986-1988. individual faecal analyses (mean: 27.9 per herd) from eight age categories of swine showed that ascaris suum, oesophagostomum spp., isospora suis, and eimeria spp. were common, while trichuris suis and strongyloides ransomi-like eggs occurred sporadically. large fatteners and gilts were most frequently infected with a. suum with maximum prevalences of 25-35% in dk, n and ... | 1998 | 9650867 |
| molecular cloning, expression and characterization of an ascaris inhibitor for pepsin and cathepsin e. | molecular cloning of a cdna for a pepsin inhibitor in the round worm, ascaris suum, was achieved. the orf was found to encode a 20-residue potential signal peptide and a 149-residue inhibitor moiety. northern analysis showed the mrna for the inhibitor to be expressed in the body wall and not in the viscera. to obtain the active inhibitor, we constructed a yeast expression vector, pyes2api, containing the inducible galactosidase promoter and a dna fragment encoding a fusion protein of the yeast a ... | 1998 | 9654082 |
| comparative effects of a fixed combination of reproterol hydrochloride and disodium cromoglycate with each agent alone on antigen-induced airway responses in sheep. | the purpose of this study was to compare the effectiveness of an eight day treatment with clinically relevant doses of a fixed combination of the beta 2 mimetic reproterol hydrochloride and disodium cromoglycate with each agent given alone against antigen-induced early (ear) and late airway responses (lar) as well as post-antigen-induced airway hyperresponsiveness (ahr) in allergic sheep. animals were treated in a randomized fashion with either the inhaled combination (n = 6), reproterol hydroch ... | 1998 | 10101743 |
| effect of chronic antigen inhalation in guinea pigs. | to examine the role of airway inflammation in airway hyperresponsiveness (ahr), we examined the effect of chronic antigen inhalation in sensitized guinea pigs. guinea pigs were actively sensitized with dinitrophenylated ascaris suum extract (dnp-as) and repeatedly exposed to aerosolized dnp-as antigen once a day for 4 or 10 days. twenty-four hours after the last antigen exposure, airway responsiveness to inhaled acetylcholine (ach) and bronchoalveolar lavage (bal) were studied. the guinea pigs r ... | 1998 | 9513585 |
| inhibition of antigen-induced airway hyperresponsiveness by ultralow molecular-weight heparin. | unfractionated heparin (uf-heparin) has been shown to prevent antigen-induced airway hyperresponsiveness (ahr), but it is ineffective when administered after the antigen challenge. we hypothesized that the failure of uf-heparin to modify postantigen ahr might depend on molecular weight. we therefore studied the effects of uf-heparin and three low-molecular-weight heparin fractions (medium-molecular-weight heparin [mmwh]; low-molecular-weight heparin [lmwh]; and ultralow-molecular-weight heparin ... | 1998 | 9517607 |
| ascaris suum: protein phosphotyrosine phosphatases in oocytes and developing stages. | protein tyrosine phosphatases were analyzed in oocytes of ascaris suum. phosphatases dephosphorylating modified acidic lysozyme were present in high-molecular-weight form (m(r) > 600,000) and as a 50- to 55-kda protein in the soluble fraction. the low-molecular-weight form of the phosphatase cross-reacted with an antiserum raised against human t-cell protein tyrosine phosphatase and was not distinguishable from the 50- to 55-kda protein tyrosine phosphatase previously described in the muscular l ... | 1998 | 9538868 |
| modulation of the motility of the vagina vera of ascaris suum in vitro by fmrf amide-related peptides. | ascaris suum contains a large number of fmrfamide-related peptides (farps) of which knefirfamide (af1), kheylrfamide (af2) and ksaymrfamide (af8, also called pf3) have been extensively studied and are known to exert actions on somatic muscle strips of the worm. in the present study, the effects of af1, af2 and af8 on the activity of the vagina vera of female a. suum have been examined in vitro. the vagina vera is a muscular tube connecting the uterus and vagina uteri to the gonopore and is proba ... | 1998 | 9550221 |
| differential effects of cyclosporine a after acute antigen challenge in sensitized cats in vivo and ex vivo. | 1. we determined the effect of cyclosporine a (csa) treatment on mast cell degranulation and lung resistance (r(l)) in vivo, and tracheal smooth muscle (tsm) contraction ex vivo after antigen challenge in sensitized cats. we also determined the direct effects of addition of csa to the tissue bath on antigen-induced responses of tsm in vitro. 2. cats (n=10) were sensitized by i.m. injection of ascaris suum antigen (aa); 5 cats (csa+) received csa twice daily for 2 weeks before acute antigen chall ... | 1998 | 9559905 |
| expression, purification, and characterization of the recombinant pepsin inhibitor from ascaris suum. | 1998 | 9561246 | |
| x-ray crystallographic studies of the complex between porcine pepsin and the aspartic proteinase inhibitor pi-3 from the nematode ascaris suum. | 1998 | 9561247 | |
| biophysical model for organic acid excretion in ascaris suum. | to develop a model for the mechanisms of organic acid excretion in nematodes, we measured the concentrations of volatile fatty acids (vfas), ph and electrical potentials across hypodermal and muscle membranes and across the composite body wall (consisting of hypodermis, muscle and cuticle) of ascaris suum using standard chromatographic and microelectrode recording techniques. in incubates containing one parasite in 20 ml modified ascaris ringer's solution, the level of combined vfas excreted int ... | 1998 | 9662703 |
| the role of camp in the actions of the peptide af3 in the parasitic nematodes ascaris suum and ascaridia galli. | af3 (avpgvlrfamide) is an endogenous rfamide-like peptide isolated from the parasitic nematode ascaris suum. it has a potent and long lasting excitatory effect in a. suum and ascaridia galli. this is mediated by a mechanism independent of the nicotinic-like acetylcholine (ach) receptor, which mediates excitatory transmission at the neuromuscular junction of both nematodes. in addition, af3 has been found to sensitise a. suum muscle to the contractile effect of ach. in this study, the involvement ... | 1998 | 9662710 |
| strategies for preliminary characterization of novel amphoteric glycosphingolipids. | 1998 | 9664355 | |
| structural analysis and immunohistochemical localization of two acidic glycosphingolipids from the porcine, parasitic nematode, ascaris suum. | the acidic glycolipid fraction (af) of the porcine, parasitic nematode, ascaris suum , consisted of two subfractions. the major component af ii reacted with orcinol-sulfuric acid and molybdate, while the minor component af i gave a positive reaction with azure-a, a cationic dye specific for sulfatides. sugar constituent analysis, methanolysis, methylation analysis, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, liquid secondary-ion mass spectrometry, and gas-liquid ... | 1998 | 9675222 |
| single-strand conformation polymorphism (sscp)-based mutation scanning approaches to fingerprint sequence variation in ribosomal dna of ascaridoid nematodes. | in this study, we assessed single-strand conformation polymorphism (sscp)-based approaches for their capacity to fingerprint sequence variation in ribosomal dna (rdna) of ascaridoid nematodes of veterinary and/or human health significance. the second internal transcribed spacer region (its-2) of rdna was utilised as the target region because it is known to provide species-specific markers for this group of parasites. its-2 was amplified by pcr from genomic dna derived from individual parasites a ... | 1998 | 9694283 |
| natural ascaris suum infections in swine diagnosed by coprological and serological (elisa) methods. | paired samples of faeces and blood were collected from weaners (w), fatteners (f), lactating sows (s) and piglets (p) in 20 danish sow herds. the samples were examined by a mcmaster technique for ascaris suum eggs and an indirect elisa for anti-a. suum igg. the coprological and serological results were significantly correlated for w and f (p < 0.0001) but not for s (p = 0.35). the coproprevalences were much lower (w 4.0%, f 15.5%, s 7.4%) than the seroprevalences (w 20.3%, f 50.5%, s 65.4%). thu ... | 1998 | 9694368 |
| dna extraction from ascaris suum muscle tissue. | a new method for the extraction of dna from ascaris suum muscle has been developed. it combines a standard sds-based extraction with a plant dna extraction procedure. the use of sds and proteinase k allows the elimination of proteins, while ctab and polyclar at eliminate glycogen and polyphenols. the dna thus obtained can easily be digested by endonucleases and amplified by pcr. | 1998 | 9694380 |
| immunocytochemical localization of a putative inhibitory amino acid receptor subunit in the parasitic nematodes haemonchus contortus and ascaris suum. | a rabbit antiserum was raised against a synthetic peptide corresponding to a region near the n-terminus of the haemonchus contortus inhibitory amino acid receptor subunit, hg1. the antiserum recognized a recombinant form of the n-terminal domain of the subunit on western blots and reacted with the ventral nerve cord of h. contortus in immunofluorescence experiments. immunofluorescence was also detected in specific head neurons of h. contortus: these were tentatively identified as ring motor- and ... | 1998 | 9695104 |
| effect of protease class-specific inhibitors on in vitro development of the third- to fourth-stage larvae of ascaris suum. | third-stage larvae (l3) of ascaris suum develop and molt to fourth-stage larvae (l4) during in vitro cultivation; consistently greater than 80% of the larvae develop to l4 during 7 days in culture (dic). to assess the role of proteases in this process, the effect of protease class-specific inhibitors was studied. the presence of either a serine protease inhibitor (aebsf, 100 microm) or an aspartic protease inhibitor (pepstatin a, 100 microm) had no effect on the percentage of l4 after 7 dic. how ... | 1998 | 9714194 |
| budesonide affects allergic mucociliary dysfunction. | airway inflammation characterized by neutrophils and free elastase contributes to allergic mucociliary dysfunction. glucocorticosteroids are the most important anti-inflammatory agents used in the treatment of asthma, but their effect on allergic mucociliary dysfunction is not known. therefore, we assessed both the prophylactic and therapeutic effects of the glucocorticosteroid budesonide on antigen-induced mucociliary dysfunction in sheep. tracheal mucus velocity (tmv), a marker of mucociliary ... | 1998 | 9729587 |
| assembly and architecture of invertebrate cytoplasmic intermediate filaments reconcile features of vertebrate cytoplasmic and nuclear lamin-type intermediate filaments. | the two major intermediate filament (if) proteins from the esophagus epithelium of the snail helix pomatia and the two major if proteins from muscle tissue of the nematode ascaris suum were investigated under a variety of assembly conditions. the lowest-order complexes from each of the four protostomic invertebrate (p-inv) if proteins are parallel, unstaggered dimers involving two-stranded alpha-helical coiled coil formation of their approximately 350 amino acid residue central rod domain (i.e. ... | 1998 | 9737925 |
| [the effectiveness of different disinfectants based on p-chloro-m-cresol against ascaris suum eggs under laboratory conditions]. | the devitalizing effectiveness of 5 patterns of the disinfectant neopredisan (active principle: p-chlorine-m-cresol) was tested to eggs of ascaris suum in coincidence with the "guidelines for the testing of chemical disinfectants" of the german veterinary society (gvs). in the suspension-test all examined patterns indicated a 100% inhibition of development ascaris suum-eggs. in the germ carrier test all patterns were in conformity with the necessities of the gvs-guidelines. also in the modified ... | 1998 | 9741186 |
| relative concentrations of heavy metals in the parasites ascaris suum (nematoda) and fasciola hepatica (digenea) and their respective porcine and bovine definitive hosts. | the concentrations of lead and cadmium determined by electrothermal atomic absorption spectrometry were significantly higher in the liver and kidney, respectively, of pigs than in their intestinal nematode parasites ascaris suum. there was no clear pattern in the distribution of lead within the ascarids, but cadmium concentrations were highest in the intestine. a parallel investigation of cattle naturally infected with the liver fluke, fasciola hepatica, revealed interesting differences. althoug ... | 1998 | 9762561 |
| the mitochondrial genome of onchocerca volvulus: sequence, structure and phylogenetic analysis. | the complete dna sequence of the mitochondrial genome of onchocerca volvulus is described. the o. volvulus mitochondrial genome is 13747 bp, slightly smaller than the mitochondrial genomes of the nematodes ascaris suum and caenorhabditis elegans, and the smallest metazoan mitochondrial dna molecule reported to date. the o. volvulus mitochondrial genome contains genes for two ribosomal rnas, 22 transfer rnas and 12 proteins. consistent with the small size of the genome, four gene pairs overlap an ... | 1998 | 9763293 |
| immuno-cross-reactivity of cut-1 and cuticlin epitopes between ascaris lumbricoides, caenorhabditis elegans, and heterorhabditis. | cuticlin is the insoluble residue of nematode cuticle. it has been proved that cuticlin and cut-1-like epitopes are conserved between the free-living caenorhabditis elegans and the entomopathogenic nematode heterorhabditis sp. the cloning of a cut-1 homologous gene from the animal intestinal parasite ascaris lumbricoides has allowed us to extend the study of immuno-cross-reactivity at the ultrastructural level to this important species. antibodies against recombinant cut-1 protein and against cu ... | 1998 | 9774538 |
| primary sequence and activity analyses of a catalase from ascaris suum. | a complete cdna encoding the catalase (ec 1.11.1.6) has been isolated from the parasitic nematode ascaris suum (ascat). the active-site residues, the residues involved in ligand interaction, and nadph-binding residues of the bovine liver catalase-type enzyme are highly conserved in the ascat predicted amino acid sequence. to confirm that the ascat cdna encodes a functional enzyme, active recombinant protein (rascat) was produced in a procaryotic expression system. the subunit molecular mass of t ... | 1998 | 9803413 |
| the immunosuppressive activity of ascaris suum is due to high molecular weight components. | the role of isolated components obtained by gel filtration chromatography of ascaris suum body extract (asc) on the modulation of the immune response to ovalbumin (ova) was evaluated and correlated with the immunogenic properties of such components. we showed that high (pi), but not low (piii), molecular weight components have the ability to inhibit ova-induced immediate and dth reactions, lymph node (ln) cell proliferation, cytokine (il-2, interferon-gamma (ifn-gamma), il-4 and il-10) and antib ... | 1998 | 9822283 |
| purification and characterisation of a secreted aminopeptidase from adult ascaris suum. | a metalloaminopeptidase was identified in culture fluids collected during in vitro cultivation of adult ascaris suum. the enzyme was purified by anion-exchange and size-exclusion hplc. the m(r) of the enzyme was estimated at 293 kda and consisted of subunits with m(r)s of 153 and 142kda. the isoelectric point of the aminopeptidase was 4.7. the aminopeptidase displayed a substrate preference for terminal arginyl residues. aminopeptidase activity was also present in muscle, female reproductive tis ... | 1998 | 9846604 |
| comparative study of histamine release from skin mast cells dispersed from atopic, ascaris-sensitive and healthy dogs. | atopic dermatitis results from the interaction between allergen and allergen-specific ige bound to the mast cell surface receptors. this process triggers mast cell degranulation and accounts at least for early phase reaction. furthermore, there is increasing in vitro and in vivo evidence that ige has the ability to induce overexpression of the fc epsilonri receptor on the mast cell plasma membrane. in order to study the potential effect of an increase in serum ige on mast cell activity, the hist ... | 1998 | 9847019 |
| prevalence of anti-toxocara antibodies in a random sample of inpatients at a children's hospital in vitória, espírito santo, brazil. | in the streets of vitória, in the state of espírito santo, brazil, are large number of stray dogs, many of which are infected with toxocara canis, suggesting a high risk for human infection. in order to investigate the prevalence of toxocara infection in children in espírito santo we studied the prevalence of anti-toxocara antibodies in 100 random inpatients over one year of age, at the children's hospital n.s. da glória, the reference children's hospital for the state. all the sera were collect ... | 1998 | 9876441 |
| solution structure of the motile major sperm protein (msp) of ascaris suum - evidence for two manganese binding sites and the possible role of divalent cations in filament formation. | the major sperm protein (msp) of ascaris suum mediates amoeboid motility by forming an extensive intermeshed system of cytoskeletal filaments analogous to that formed by actin in many other amoeboid cells. msp is a dimeric molecule that polymerizes to form non-polar filaments constructed from two helical subfilaments that wind round one another. moreover, msp filaments can interact with one another to form higher-order assemblies without requiring the range of accessory proteins usually employed ... | 1998 | 9878374 |
| distribution of ascaris suum in experimentally and naturally infected pigs and comparison with ascaris lumbricoides infections in humans. | this paper describes the distribution of ascaris suum in experimentally and naturally infected pigs, and offers a comparison with a. lumbricoides infections in humans. in the first study, worms were recovered post-mortem from a group of 38 pigs that had been trickle inoculated with 10,000 infective a. suum eggs twice weekly for 12 weeks. in the second study, worms were collected from a group of 49 pigs that had been kept on a pasture contaminated with infective a. suum eggs for 10 weeks, after w ... | 1998 | 9881384 |
| interactions between the nematode parasite of pigs, ascaris suum, and the earthworm aporrectodea longa. | pig faeces in which ascaris suum eggs had been embryonating for 57 days were placed in buckets of soil containing either 30 or no earth-worms (aporrectodea longa). when present, earthworms consumed the faeces and transported the eggs down into the soil, without inflicting any visible damage on the eggs. in later experiments 10 earthworms from the above experiment were fed to each of ten pigs, and another 40 earthworms were dissected. none of the 10 pigs became infected with a. suum through consu ... | 1998 | 9926459 |
| recovery and distribution of ascaris suum superimposed on a schistosoma japonicum infection in pigs. | the aim of this study was to examine the effect of a primary patent schistosoma japonicum infection on the establishment and location of a superimposed ascaris suum infection in pigs. the study comprised two experiments each containing two groups of pigs. in the first experiment, 7 pigs were injected intramuscular (i.m.) with 800 s. japonicum cercariae and inoculated with 1,000 a. suum eggs 11 weeks post primary infection (ppi) and 8 pigs were inoculated with 1,000 a. suum eggs at the time of ch ... | 1998 | 10772553 |
| [sequencing and homology analysis of cdnas encoding fhgst and asgst]. | to study the homologies between fhgst, asgst and sj26gst. | 1998 | 12078253 |
| ultrastructural changes in ascaris suum after oxibendazole treatment. | the effect of oxibendazole treatment on adult ascaris suum was assessed using scanning and transmission electron microscopy. the changes included collapse of the lip papillae, rupture of buccal cavity cuticle, prolapse of the pharynx, degeneration of epithelial cells and erosion of microvilli. theses changes may lead to impaired digestion and absorption of nutrients and cause cellular autolysis, resulting in the death of the worm. | 1998 | 17580993 |
| use of polymerase chain reaction in the diagnosis of toxocariasis: an experimental study. | in this paper we report the usefulness of polymerase chain reaction technique in the diagnosis of visceral larva migrans in a mouse model. liver samples obtained from two set of experimentally infected mice (10, 100, 1,000 and 10,000 embryonated toxocara canis eggs per mouse) along with the eggs of t. canis, t. cati and ascaris suum were included in this study. polymerase chain reaction (pcr) was performed using toxocara primers (sb12). the first pcr product electrophoresis revealed very thin po ... | 1997 | 9561605 |
| the effects of the peptides af3 (avpgvlrfamide) and af4 (gdvpgvlrfamide) on the somatic muscle of the parasitic nematodes ascaris suum and ascaridia galli. | af3 (avpgvlrfamide) and af4 (gdvpgvlrfamide) are endogenous rfamide-like peptides isolated from the parasitic nematode ascaris suum. here the actions of these peptides on the somatic musculature of ascaris have been investigated and compared to the action of acetylcholine (ach), the excitatory transmitter at the neuromuscular junction. ach, af3 and af4 contracted muscle with ec50s of 13 +/- 1 microm, 24 +/- 6 nm and 37 +/- 2 nm, respectively (n = 6). the muscle cells were depolarized by ach (3 m ... | 1997 | 10190177 |
| an outbreak of ascariasis with marked eosinophilia in the southern part of kyushu district, japan, caused by infection with swine ascaris. | ascariasis has been a representative soil-transmitted intestinal parasitic disease in warm climates. in japan, this disease was a major and serious public health problem only a few decades ago. however, the incidence of the disease nowadays is reportedly less than 0.01%. recently in 1994 through 1995, we experienced a total of 14 cases who were suspected as having ascariasis. they were characterized by peripheral blood eosinophilia (30-70%), high serum titers against ascaris antigen, and most no ... | 1997 | 9656376 |
| cross-reactions between toxocara canis and ascaris suum in the diagnosis of visceral larva migrans by western blotting technique. | visceral larva migrans (vlm) is a clinical syndrome caused by infection of man by toxocara spp, the common roundworm of dogs and cats. tissue migration of larval stages causes illness specially in children. because larvae are difficult to detect in tissues, diagnosis is mostly based on serology. after the introduction of the enzyme-linked immunosorbent assay (elisa) using the larval excretory-secretory antigen of t. canis (tes), the diagnosis specificity was greatly improved although cross-react ... | 1997 | 9661302 |
| seroepidemiology of abdominal angiostrongyliasis: the standardization of an immunoenzymatic assay and prevalence of antibodies in two localities in southern brazil. | abdominal angiostrongyliasis is a nematode disease produced by angiostrongylus costaricensis, a metastrongylid parasite of wild rodents. accidental human infection occurs through ingestion of food or water contaminated with third-stage larvae present in the mucous secretion of terrestrial molluscs. an elisa test was standardized for detection of igg antibodies recognizing a surface antigen prepared from female worms. competitive absorption of sera with ascaris suum crude antigen resulted in a te ... | 1997 | 9491104 |
| altered phosphorylation/inactivation of a novel pyruvate dehydrogenase in adult ascaris suum muscle. | 1997 | 9497054 | |
| cloning and characterization of a putative testis-specific pyruvate dehydrogenase beta subunit from the parasitic nematode, ascaris suum. | 1997 | 9497067 | |
| helminth surveillance as a prerequisite for anthelmintic treatment in intensive sow herds. | helminth prevalences in pigs in intensive production systems are often very low due to housing and management factors, and the present study was carried out to examine whether routine anthelmintic treatment in such herds can be replaced by coprological surveillance of the helminth status combined with anthelmintic treatment when necessary. after an initial examination, 25 out of 28 large herds of danish sows (> or = 50 sows, production of fatteners) were found to have such light helminth infecti ... | 1997 | 9477500 |
| ascaris suum prevalence and intensity: an abattoir survey of market hogs in saskatchewan. | two surveys examining the prevalence and intensity of ascaris suum were conducted at a saskatchewan abattoir. in the first, 2500 livers from market weight animals were examined and scored for lesions consistent with ascarid migration. forty-four percent displayed some degree of scarring with approximately 8% being severely damaged. in the second, both livers and intestinal contents from 500 market weight animals were examined. seventeen percent were infected with intestinal a. suum (mean of 2.5 ... | 1997 | 9477517 |
| use of an agar-gel technique for large scale application to recover ascaris suum larvae from intestinal contents of pigs. | four groups each of 3 pigs were inoculated with ascaris suum eggs. pigs in groups 1 and 3 were inoculated with 1000 eggs, and pigs in groups 2 and 4 with 10,000 eggs. on day 10 and 21 post-inoculation (p.i.), respectively, groups 1 + 2 and 3 + 4 were slaughtered, and the contents from the small intestines collected. the contents were mixed with agar to a final concentration of 1% agar and allowed to sediment. the larvae were allowed to migrate from the agar-gel into 38 degrees c 0.9% saline over ... | 1997 | 9444774 |
| effects of environmental pollutants on the porcine and bovine immune systems. | immunological examinations were done in 30 fattening pigs from three swine farms (d., m., t.) and 20 dairy cows from one cattle farm (n.) in the district of hodonín in 1994. at the same time, samples of stable dust deposits collected in the four farms were analysed for the contents of mercury, cadmium, lead, gamma-hch (lindane), and total ddt and polychlorinated biphenyls (pcb). decreased blood level of lysozyme (0.92 mg/l) was found in the swine herd d. ten percent of the pigs in the herd were ... | 1997 | 9446971 |
| effects of concurrently administered copper and mercury on phagocytic cell activity and antibody levels in guinea pigs with experimental ascariasis. | the subchronic effect of copper and mercury on selected immunological parameters in guinea pigs with experimental ascariasis was studied. cupric sulphate and mercuric chloride administered for 28 days did not suppress the levels of specific circulating antibody in a subsequent ascaris suum infection. intoxication of animals significantly inhibited the phagocytic activity of peritoneal macrophages throughout the experiment. ascaris suum infection elevated macrophage phagocytic activity but values ... | 1997 | 9443953 |
| comparison of methods for recovery of ascaris suum larvae from tissues of mice. | experiments were undertaken to compare procedures for isolating ascaris suum from mice. a standardised intragastric procedure for inoculation of a. suum eggs, which had a very low interdose variation (s.d. = 6.2% of mean inoculation dose), was developed. there was no significant difference in the number of larvae recovered from the large intestine 4 h post inoculation (p.i.) by using either baermannisation of washed intestinal wall or submucosal scrapings. in contrast, a significantly higher rec ... | 1997 | 9421715 |
| myogenic and neurogenic mechanisms and arachidonate metabolites in bronchial muscle response to allergen. | we investigated allergen-induced airway hyperresponsiveness (ah) in bronchial tissues obtained from dogs that inhaled ascaris suum leading to ah (resp) in vivo or that exhibited no change (non-resp) as well as from dogs that inhaled saline (sham). resp tissues were not hyperresponsive to kcl or to carbachol, whereas contractions to electrical field stimulation (efs) were reduced. this reduction was reversed partially by indomethacin and completely by replacement of the bathing fluid. radioimmuno ... | 1997 | 9435565 |
| effect of tyb-2285 on passive cutaneous anaphylaxis in rats. | 1. tyb-2285 (1-30 mg/kg p.o.) inhibited ovalbumin (oa)- and dinitrophenyl-ascaris (dnpas)-induced passive cutaneous anaphylaxis (pca) in a dose-dependent manner. 2. the ed50 of tyb-2285 and ketotifen fumarate on oa-induced pca were 0.5 and 3.9 mg/kg, respectively. the ed50 of typ-2285 and amlexanox on dnp-as-induced pca were 3.5 and 0.9 mg/ kg, respectively. 3. tyb-2285 (3-30 mg/kg p.o.) inhibited histamine consumption at the pca site. 4. unlike cyproheptadine or amlexanox, tyb-2285 (30 mg/kg p. ... | 1997 | 9013210 |