Publications

attempts to induce protective immunity in hamsters against infection by a liver fluke of man (opisthorchis viverrini).	the development of acquired resistance in opisthorchiasis was studied in hamsters experimentally infected with opisthorchis viverrini. the induction of protective immunity was attempted by first exposing adult female golden syrian hamsters to 1, 2 or 3 doses of infective metacercariae obtained from naturally infected cyprinoid fishes and then reinfecting them with 80 metacercariae. in other experiments, animals that were infected with 50 metacercariae were treated with praziquantel prior to bein ...	1983	6835694
liver collagen turnover in hamsters during infection by the human liver fluke, opisthorchis viverrini.	hamsters infected with opisthorchis viverrini, a liver fluke of man, showed an increased deposition of collagen in their livers. however, increased collagen breakdown as well as its synthesis were observed in the infected livers. thus, stimulated synthesis might be the main factor responsible for the net increase in collagen content. synthesis and degradation increased to a greater extent in short-term infection than in long-term infection whereas the hepatic collagen content was equally elevate ...	1983	6877280
ultrastructural investigations on the effects of praziquantel on human trematodes from asia: clonorchis sinensis, metagonimus yokogawai, opisthorchis viverrini, paragonimus westermani and schistosoma japonicum.	the effect of praziquantel (biltricide) on the ultrastructure of trematodes pathogenic to man in asia was investigated in: clonorchis sinensis, opisthorchis viverrini, schistosoma japonicum, metagonimus yokogawai, and paragonimus westermani. the different parasites were isolated from their respective experimental hosts and uniformly incubated for 5, 15, 30 or 60 min at 37 degrees c in medium tc 199 containing 0, 1, 10 or 100 micrograms praziquantel/ml. all parasites exposed to praziquantel were ...	1983	6338885
a comparison of a modified quick-kato technique and the stoll dilution method for field examination for opisthorchis viverrini eggs.	a modified quick-kato technique and the stoll dilution method for the diagnosis of opisthorchis viverrini infection were compared from the standpoints of both replicability and sensitivity. one stoll dilution preparation and two modified quick-kato smears were prepared from single stool specimens collected from 221 persons in a village in north-eastern thailand. stool examinations were made in duplicate for each technique by two examiners, and the averages of the raw egg counts were used to esti ...	1983	6630991
humoral immune responses in hamsters infected with opisthorchis viverrini.	the kinetics and nature of humoral immune responses to somatic and excretory-secretory (es) antigens were investigated in hamsters experimentally infected with different numbers of opisthorchis viverrini. es antigens were obtained from the in vitro culture of adult flukes and somatic antigens were aqueous extracts of adult flukes. antibodies in the serum and bile of infected animals were determined by the microhaemagglutination technique, using glutaraldehyde fixed sheep red blood cells sensitiz ...	1983	6635763
opisthorchis viverrini: the relationship between egg production, worm size and intensity of infection in the hamster.	worm recoveries, egg production and worm lengths were determined in golden syrian hamsters given between five and 200 opisthorchis viverrini metacercariae. animals given five, 10 or 20 metacercariae showed similar worm recovery rates of 38%, 36% and 37%, respectively, whereas animals given 50, 100 or 200 metacercariae gave recoveries of 49%, 65% and 54%, respectively. the distribution of worms between right, left and middle liver lobes was similar for animals with between one and 43 worms but di ...	1983	6636282
liver collagen in opisthorchis viverrini infected hamsters following praziquantel treatment.	the dose of praziquantel required to achieve a 100% worm reduction in o. viverrini infected hamsters was found to be 300 mg/kg body weight. the drug was administered orally for 1 day by dividing a total dose into 3 equal doses at 4 h interval. the effect of praziquantel treatment on liver collagen was followed by measuring liver collagen content in at various intervals after administration of the drug. a decrease in collagen content in the infected livers occurred within a few weeks following th ...	1983	6658499
effect of culture media on production of excretory-secretory products and egg output of opisthorchis viverrini in vitro.	adult opisthorchis viverrini were maintained metabolically active in vitro for an extended period in earle's basal medium (bme). the worms were most active during the first 7 to 10 days of incubation and the culturing fluids were found to react in immunodiffusion with antisera. the mean survival period and egg output per worm per day could be enhanced when bme was supplemented with 5% normal, human bile but not with the same concentration of bile ultrafiltrate. a similar degree of enhancement wa ...	1982	6897077
morbidity in relation to intensity of infection in opisthorchiasis viverrini: study of a community in khon kaen, thailand.	a study of the prevalence and intensity of opisthorchiasis viverrini in relation to morbidity as determined by standard medical examination was carried out in nong ranya, a small village containing 309 people in northeastern thailand. opisthorchis viverrini infection as determined by stoll's quantitative egg count method had an overall prevalence of 94% and reached 100% prevalence in most age groups above the age of 10 years. with respect to intensity, 6% were uninfected, 26% had light (less tha ...	1982	6983303
alpha 1-antitrypsin, alpha 1-antichymotrypsin and alpha 2-macroglobulin in human liver fluke (opisthorchiasis).	in mild and moderate opisthorchis viverrini infection it is difficult to find clinical signs for diagnosis. also routine clinical chemical reactions often fail to demonstrate changes in these patients. this study showed a significant increase of serum proteinase inhibitors, alpha 2-macroglobulin, alpha 1-antitrypsin and alpha 1-antichymotrypsin in patients suffering from o. viverrini infection compared with healthy controls. the increase of proteinase inhibitors together with other biochemical l ...	1982	6182657
potentiation by the human liver fluke, opisthorchis viverrini, of the carcinogenic action of n-nitrosodimethylamine upon the biliary epithelium of the hamster.		1982	6295426
serodiagnosis of opisthorchis viverrini infestation by an enzyme immuno-assay.	the application of an enzyme immuno-assay is evaluated with regard to antibody reactivity to opisthorchis viverrini antigen in 20 laotian patients and control groups including patients with other helminthic infections as well as persons without parasitic infestation. it is concluded that the enzyme immuno-assay indicates infection by o. viverrini. in certain circumstances, which are discussed, the test may serve for valuation of treatment.	1982	7048673
anti-p1, allohemagglutinins associated with clonorchis sinensis and opisthorchis viverrini infections in patients from southeast asia.	an automated assay of anti-p1 allohemagglutinins has been carried out on sera of 61 individuals from southeast asia : 28 with clonorchiasis, 18 with opisthorchiasis and 15 control subjects. anti-p1 activity was detected in 61% of the opisthorchiasis sera, 57% of the clonorchiasis sera and in 26.6% of the control subjects. their concentration, in the sera, was low in control subjects and exceptionally high in clonorchiasis and opisthorchiasis (up to 13 and 22 times the maximum concentration of th ...	1982	7051340
opisthorchis viverrini : intensity and rates of infection in cyprinoid fish from an endemic focus in northeast thailand.	the infection rates and monthly variations in intensities of opisthorchis viverrini infection in cyprinoid fish from an endemic focus in northeast thailand were investigated between april 1980 and march 1981. out of six species of cyprinoid fish examined, four were found to harbour o. viverrini metacercariae. all four species of infected fish showed high rates of infection. among the four species of o. viverrini infected fish, cycloccheilicthys apagon and puntius leiacanthus were found in abunda ...	1982	7112213
acquired resistance to opisthorchis viverrini in the hamster.	two groups of ten hamsters received a primary challenge of five opisthorchis viverrini metacercariae intragastrically (groups i and iii) and a third group was left untreated (group ii). 90 days after the primary challenge groups i and ii were given a further 50 metacercariae. animals were killed 35 days after the secondary challenge and worm burdens and faecal egg counts evaluated. compared with group ii animals, group i showed a 24.88% reduction in mean liver worm burden, a difference which was ...	1982	7164147
effects of physico-chemical factors on the infection of hamsters by metacercariae of opisthorchis viverrini.		1982	7170645
[praziquantel in the treatment of far eastern hepatic distomiasis caused by clonorchis sinensis or opisthorchis viverrini].	forty-six patients infected with opistorchis viverrini and/or clonorchis sinensis, 24 males and 22 females, all laotian or vietnamese, aged 7 to 68, weighing 18 to 70 kg, were detected by parasitological stool examinations, numeration of eggs per gram of feces (e.p.g.). in this manner we observed 23 light infestations (1-999 e.p.g.), 20 moderate infestations (10,000-29,999 e.p.g.). before treatment, all these patients were submitted to a complete history and clinical examination with biological ...	1981	7296740
the in vitro effects of serum on the adults, metacercariae, and eggs of opisthorchis viverrini.	adult and juvenile opisthorchis viverrini liver-flukes incubated in hamster chronic infection serum (cis) developed tegumental precipitates which enclosed the entire parasite. adult worms appeared dead by 48 hours in cis and juvenile worms by 18 hours. no such reactions were observed in normal hamster serum (nhs). heat inactivation of cis at 56 degrees c abolished the precipitin reactivity against both adult and juvenile parasites though reactivity was regained upon addition of nhs or guinea-pig ...	1981	7330943
treatment of opisthorchis viverrini with mebendazole.	thirty-six hospitalized thais (21 males, 15 females, 9 to 63 years of age) with opisthorchis viverrini infection were treated with mebendazole in dosages of 20 mg/kg body weight for 4 weeks and 30 mg/kg body weight for 3 or 4 weeks. the drug was found to be effective when given in dosages of 30 mg/kg body weight for 3 to 4 weeks only a few eggs being found in the stool of one of 27 persons at 3 or 4 weeks post-treatment. in follow-up examination at 6 months, only two of 15 persons available were ...	1981	7344109
field trial of praziquantel in human opisthorchiasis in thailand.	praziquantel is an excellent drug for treatment against opisthorchis viverrini infection. sixty cases of opisthorchis viverrini infection were treated with praziquantel, 3 x 25 mg/kg body weight for one day, except one case that was retreated with the same regimen at one month after the first treatment, and the cure rate was 100.0% at one year after the treatment. the side effects of the drug were headache, lassitude, sleepiness, and diarrhoea, but most of these were only in mild degree. other l ...	1981	7344110
praziquantel in clonorchiasis and opisthorchiasis.	a single stool examination revealed pathogenic intestinal parasites in 462 (58%) of 796 vietnamese and cambodian refugees. 56 (7.0%) were infected with clonorchis sinensis and/or opisthorchis viverrini. these patients received praziquantel in a dosage of 20 mg/kg bwt. p.day on 3 consecutive days. parasitological controls were completed after 12 months. no further excretion of eggs could be detected in 88% of the patients. concurrent infections with other trematodes and cestodes were also cured. ...	1981	7345688
studies on the chemotherapy of human opisthorchiasis: ii. clinical trial of niclofolan.	a clinical trial of niclofolan on human opisthorchiasis was carried out in 60 patients admitted to the hospital for tropical diseases. two dosage regimen, 2 mg and 3 mg per kg body weight repeated after a 72 hour interval was administered to 31 and 4 patients respectively. comparative evaluation with the placebo group of 25 patients was made by the stoll count on day 20, 40 and 60 after treatment. on day 60 percentage egg reduction of the treated and the placebo groups were similar. mild and tra ...	1981	7020093
liver-fluke infection as an aetiological factor in bile-duct carcinoma of man.	this paper reviews, with particular reference to opisthorchis viverrini, the evidence that opisthorchiasis and clonorchiasis are aetiological factors in the causation of human bile-duct cancer, especially cholangiocarcinoma, and considers the other aetiological factors which may be operating. the epidemiology, pathology in man and experimental animals, and histogenesis of cholangiocarcinoma associated wtih liver-fluke infection are also considered.	1981	6277052
[clinical trial of niclofolan (bay 9015) in the treatment of human opisthorchiasis. apropos of 51 cases].	51 refugees from south east asia infected with opisthorchis viverrini or clonorchis sinensis were included in a therapeutic study with niclofolan (bay 9015) at a dose of 1 mg/kg body weight given three times at intervals of 48 hours. clinical and biological tolerance was carefully controlled and in all cases proved to be very good or excellent particularly in several patients with various haemoglobinopathies or erythrocytal enzymopathies (g-6-pd deficiency). of the total of 51 cases, 17 were cur ...	1980	7014011
helminths in the induction of cancer: opisthorchis viverrini, clonorchis sinensis and cholangiocarcinoma.	opisthorchis and clonorchis parasitize the bile ducts of millions of persons in the far east. the most important aspect of infection with these flukes is their ability to initiate cancer. numerous studies have shown these flukes to occur in association with cholangiocarcinoma far more frequently than can be explained by chance. experimental studies in animals have confirmed the carcinogenic potential of these parasites. although the pathogenesis remains unclear, the initial carcinogenic event is ...	1980	6252671
opisthorchis viverrini: liver changes in golden hamsters maintained on high and low protein diets.	two groups of hamsters maintained on eight high (25.6%) or low (5.3%) content protein diets were infected with 50 opisthorchis viverrini metacercariae by intragastric inoculation. three animals from each group were sacrificed at 14-day intervals over a 32-week period. two groups of non-infected control animals maintained on identical diets were killed at similar intervals. histological examination revealed qualitatively similar pathological responses to the parasite in both diet groups, but over ...	1980	6110324
[intestinal helminths in bangkok stray dogs and their role in public health (author's tranls)].	feces examination of 107 stray dogs in bangkok, thailand, on intestinal helminths showed the following rates: hookworms 100%, trichuris vulpis 54.2%, toxocara canis 6.5%, gnathostoma spinigerum 2.8%, spirocerca lupi 17.8%, diphyllobothrium mansoni 1.9%, and opisthorchis viverrini 1.9%. out of these species hockworms (especially a. braziliense), t. canis, g. spinigerum, and d. mansoni are important sources of human cutaneous or visceral larva migrans, the latter two being special parasites of the ...	1980	7435002
field studies on the bionomics of bithynia siamensis siamensis and the transmission of opisthorchis viverrini in bangna, bangkok, thailand.	a field study on the bionomics of bithynia siamensis siamensis and the transmission patterns of opisthorchis viverrini was carried out in bangna, bangkok, thailand, from may 1975 to april 1976. snail populations were found to fluctuate according to rainfall. during the high rainfall period where snail habitats were deep, snail populations recovered at the water surface were higher than those recovered on the mud bottom; during the low rainfall perod where snail habitats were shallow, snails were ...	1980	7444576
opisthorchis viverrini: partial success in adoptively transferring immunity with spleen cells and serum in the hamster.	three groups of golden syrian hamsters received intraperitoneally either, (1) 1 x 10(7) spleen cells, (2) 0.5 ml. serum or, (3) 1 x 10(7) spleen cells plus 0.5 ml. serum, from donors infected 6 weeks previously with 25 opisthorchis viverrini metacercariae, one day before and at the same time as challenge with 25 metacercariae. three groups of control animals received the same quantities of spleen cells, serum or an admixture of both from normal non-infected donors in the same manner followed by ...	1980	7217648
potential health hazards of the water resources development: a health survey in the phitsanulok irrigation project, nan river basin, northern thailand.	a health survey was carried out among residents of 33 villages under the phitsanulok irrigation project area, nan river basin, northern thailand, whereby general health conditions were examined including intradermal tests for schistosomiasis japonica, stools for intestinal parasites and sera tested by circumoval precipitin test for antibodies to s. japonicum and by agglutination test for leptospiral infection. health investigations revealed that 913 (60.9%) of 1,499 people examined had experienc ...	1980	7221699
effects of dimethylnitrosamine on induction of cholangiocarcinoma in opisthorchis viverrini-infected syrian golden hamsters.		1978	214229
liver changes in hamsters infected with a liver fluke of man, opisthorchis viverrini.	thirty male syrian golden hamsters were each infected with 100 metacercariae of opisthorchis viverrini. the hamsters were killed at 3, 7, 15, 30, 154 days of infection. the early pathological changes consisted of an acute inflammatory reaction involving the bile ducts of the second order and the portal connective tissue, especially the large veins, as well as focal coagulation necrosis of the liver lobules. as the flukes developed into adults they induced hyperplasia and adenomatous formations o ...	1978	686245
effects of the injectable contraceptive depot medroxyprogesterone acetate in thai women with liver fluke infestation: results after six months.	the effect of the three-monthly injectable contraceptive depot medroxyprogesterone acetate (dmpa) on liver and lipid function was assessed in thai women with liver fluke (opisthorchis viverrini) infestation, dmpa administration being started in the immediate postpartum period. immediate postpartum iud and sterilization acceptors with fluke infestation were recruited as a comparison (control) group for the fluke-positiv dmpa acceptors. comparable groups of fluke-negative acceptors were recruited ...	1977	302157
socio-economic, health and nutritional status of the villagers in the nong wai irrigation area, khon kaen, northeast thailand.	studies were carried out from june 1974 to may 1975 on the socio-economic status, health and nutritional status of the people in 4 villages, in the irrigation area of the nong wai pioneer agricultural project of khon kaen province, northeast thailand. the result obtained were compared with those in 2 non-irrigated villages in the same province, in order to identify the health and nutritional problems which might arise during the water resource development in the irrigation area. it was found tha ...	1976	1030856
the ultrastructure of helminth. vi. the body wall of opisthorchis viverrini (poirier, 1886).		1971	4333630
opisthorchis viverrini (poirier, 1886) a trematode parasite of man in west malaysia.		1969	5816416
clinical manifestations of opisthorchis viverrini infections in thailand.		1966	5959105
opisthorchis viverrini in thailand--the life cycle and comparison with o. felineus.		1965	14275209
the morphology of the metacercaria of opisthorchis viverrini, with special reference to the excretory system.		1961	13924247
studies on opisthorchis viverrini in thailand.		1955	13258561
studies on the treatment of opisthorchis viverrini in human infections with quinacrine hydrochloride and chloroquine phosphate.		1955	13268815