Publications

TitleAbstractYear(sorted descending)
Filter
PMID
Filter
nitroimidazole drugs vary in their mode of action in the human parasite giardia lamblia.giardia lamblia (syn. duodenalis, intestinalis) is a globally occurring micro-aerophilic human parasite that causes gastrointestinal disease. standard treatment of g. lamblia infections is based on the 5-nitroimidazole drugs metronidazole and tinidazole. in two other micro-aerophilic parasites, entamoeba histolytica and trichomonas vaginalis, 5-nitroimidazole drugs bind to proteins involved in the thioredoxin-mediated redox network and disrupt the redox equilibrium by inhibiting thioredoxin redu ...201224533278
axenic cultivation and comparative phospholipase a2 activity of giardia duodenalis in a serum-free medium.mammalian serum is essential for the growth of giardia duodenalis cultivated under axenic conditions. unfortunately, some factors present in bovine serum used as supplement in the culture medium may inhibit protozoal growth and activity. tyi-33-pacsr is a tyi medium supplemented with a serum replacement (pacsr) made up of earle's amino acid solution, diamond's vitamin-tween 80 mixtures and lcr (a lipid-cholesterol - rich mixture). pacsr was previously used in the culture media for axenic cultiva ...201222875669
programmed cell death in giardia.programmed cell death (pcd) has been observed in many unicellular eukaryotes; however, in very few cases have the pathways been described. recently the early divergent amitochondrial eukaryote giardia has been included in this group. in this paper we investigate the processes of pcd in giardia. we performed a bioinformatics survey of giardia genomes to identify genes associated with pcd alongside traditional methods for studying apoptosis and autophagy. analysis of giardia genomes failed to high ...201222405231
herpes simplex virus type 2 risks in female sex workers in the china-vietnam border county of hekou.to survey the prevalence and risk factors of hsv-2 among chinese and vietnamese female sex workers (fsw) in the border county of hekou, yunnan province, china.201223228841
assessment of screening practices in a subacute clinical setting following introduction of trichomonas vaginalis nucleic acid amplification testing.trichomonas vaginalis analyte-specific reagent is a highly sensitive assay for t vaginalis detection. we report how this diagnostic innovation influenced the sexually transmitted infection ordering practice patterns of 20 subacute-care clinicians.201223189457
sexually transmitted infection prevalence in symptomatic adolescent emergency department patients.this study aimed to determine the prevalence of sexually transmitted infections (stis) in symptomatic adolescent female patients presenting to a pediatric emergency department (ed). secondary objectives included determining correlates of infection.201223187982
[microbiologic basis of diagnosis and treatment of pelvic inflammatory disease].pelvic inflammatory disease (pid) is caused by a large spectrum of micro-organisms. however, the microbiological cause is unknown in approximately half of cases according to varying series. in the context of sexually transmitted disease (std), the most frequently identified microorganisms causing pid are neisseria gonorrhoeae, chlamydia trachomatis and mycoplasma genitalium. in such cases, bacterial vaginosis and trichomonas vaginalis are frequently associated. in case of complicated pid or when ...201223140621
sensitivity of gram stain in the diagnosis of urethritis in men.acute urethritis is among the most common types of sexually transmitted diseases in men. the diagnosis usually requires microscopic evidence of urethritis, but sometimes urethral pathogens are detected in asymptomatic men without such evidence. the aims of this study were to assess the sensitivity of gram stain in men with urethral symptoms and to relate it to the microorganisms isolated.201222308534
evaluation of seeplex® std6 ace detection kit for the diagnosis of six bacterial sexually transmitted infections.traditionally, the diagnosis of bacterial sexually transmitted infection (sti) has been dependent on the isolation of the causative pathogens by culturing endocervical or urethral swab specimens on selective media. while such procedures typically provide excellent diagnostic accuracy, they are often time-consuming and expensive. a multiplex polymerase chain reaction (pcr) assay, based on a semi-automated detection system, was evaluated for the detection of six sti causative organisms. the seeple ...201222252268
urethritis/cervicitis pathogen prevalence and associated risk factors among asymptomatic hiv-infected patients in south africa.to determine sexually transmitted infection (sti) prevalence, and patient characteristics associated with detection of urethritis/cervicitis pathogens, among hiv-infected individuals offered voluntary sti screening at a south african hiv treatment center.201222706215
prevalence of malaria and sexually transmitted and reproductive tract infections in pregnancy in sub-saharan africa: a systematic review.malaria and sexually transmitted infections/reproductive tract infections (stis/rtis) in pregnancy are direct and indirect causes of stillbirth, prematurity, low birth weight, and maternal and neonatal morbidity and mortality.201222665107
the utility of wet prep in predicting neisseria gonorrhoeae and chlamydia trachomatis.diagnosing chlamydia trachomatis (ct) and neisseria gonorrhoeae (ng) cervical infections can be difficult in the emergency department without real-time testing, as historical and physical elements are known to be unreliable.201222595630
prevalence and associations of genital ulcer and urethral pathogens in men presenting with genital ulcer syndrome to primary health care clinics in south africa.this study aimed to determine the prevalence of genital ulcer and urethral pathogens, as well as their association with clinical features, in men with genital ulcer disease (gud) enrolled in a clinical trial.201223064538
reproductive tract infections (rtis) among married non-pregnant women living in a low-income suburb of beirut, lebanon.this study aimed to identify reproductive tract infections (rtis) in married, non-pregnant women, aged 18 to 49 years, living in a low-income suburb of beirut, and to investigate the relationship between demographic and socioeconomic factors and these infections.201223000869
detection rates of trichomonas vaginalis, in different age groups, using real-time polymerase chain reaction.the study aimed to compare the overall detection rate of trichomonas vaginalis to chlamydia trachomatis and neiserria gonorrhea and report detection rates by age groups.201222968052
sexually transmitted infections and sexual behaviour among youth clients of hotel-based female sex workers in dhaka, bangladesh.a cross-sectional study was conducted among youth clients of hotel-based female sex workers (ychbfsws) in nine randomly selected hotels in bangladesh to examine sexual-risk behaviour, condom use and determinants of condom use in last sex, knowledge of hiv, sexually transmitted infection (sti) prevalence and sti care-seeking behaviour. a prestructured questionnaire was used to collect sociodemographic, behavioural, clinical information; urine specimens (before sex) and blood were collected for di ...201222930291
hiv and sti control in el salvador: results from an integrated behavioural survey among men who have sex with men.this cross-sectional study investigates hiv, other sexually transmitted infections (sti), and risk behaviours among men who have sex with men (msm) in two cities in el salvador.201222917694
epidemiology of chlamydia trachomatis endocervical infection in a previously unscreened population in rome, italy, 2000 to 2009.as reliable data on chlamydia trachomatis infection in italy are lacking and as there is no italian screening policy, epidemiological analyses are needed to optimise effective strategies for surveillance of the infection in the country. we collected data from 6,969 sexually active women aged 15 to 55 years who underwent testing for endocervical c. trachomatis infection at the cervico-vaginal pathology unit in the department of gynaecology and obstetrics of sapienza university in rome between 200 ...201222748006
[role of infectious agents in the emergence of malignant tumors].according to the data of the international agency for research on cancer (iarc), at least 6 virus species (hpv, ebv, hhv-8/kshv, htlv-1, hbv, hcv), 4 helminthes species (schistosoma haematobium and japonicum, opisthorchis viverrini, clonorchis sinensis) and i bacterium species (helicobacter pylori) have been proved to be capable of causing the development of cancer. the analysis of the data available shows that merkel cell polyomavirus (mcv), herpes simplex virus (hsv), john cunningham polyomavi ...201223163048
correlation of atopobium vaginae amount with bacterial vaginosis markers.the purpose of this study was to test the correlation of the amount of atopobium vaginae with the most commonly used markers for bacterial vaginosis (bv).201222371042
in vitro antimicrobial activity of persian shallot (allium hirtifolium).allium hirtifolium is a persian native plant grown in cool mountain slopes of iran. it has been used as a spice in iran for many years. according to the literature review, there are no considerable reports on the antimicrobial properties of this plant. in this study, the antimicrobial activity of persian shallot hydroalcoholic extract and f1 fraction of the plant (containing amino acid derivatives and/or other cationic compounds) was investigated on some gram positive cocci and bacilli, gram neg ...201223210319
clinical importance of detection of bacterial vaginosis, trichomonas vaginalis, candida albicans and actinomyces in papanicolaou smears.the aim of this study was to determine the role of papanicolaou (pap) smears in the diagnosis of lower genital tract infections.201223157037
Structure of the Trichomonas vaginalis Myb3 DNA-binding domain bound to a promoter sequence reveals a unique C-terminal ß-hairpin conformation.Trichomonas vaginalis Myb3 transcription factor (tvMyb3) recognizes the MRE-1 promoter sequence and regulates ap65-1 gene, which encodes a hydrogenosomal malic enzyme that may play a role in the cytoadherence of the parasite. Here, we identified tvMyb3(53-180) as the essential fragment for DNA recognition and report the crystal structure of tvMyb3(53-180) bound to MRE-1 DNA. The N-terminal fragment adopts the classical conformation of an Myb DNA-binding domain, with the third helices of R2 and R ...201221908401
Iron-saturated lactoferrin and pathogenic protozoa: could this protein be an iron source for their parasitic style of life?Iron is an essential nutrient for the survival of pathogens inside a host. As a general strategy against microbes, mammals have evolved complex iron-withholding systems for efficiently decreasing the iron accessible to invaders. Pathogens that inhabit the respiratory, intestinal and genitourinary tracts encounter an iron-deficient environment on the mucosal surface, where ferric iron is chelated by lactoferrin, an extracellular glycoprotein of the innate immune system. However, parasitic protozo ...201222191452
access to health services and sexually transmitted infections in a cohort of relocating african american public housing residents: an association between travel time and infection.background:: high incidence and prevalence of sexually transmitted infection (sti) in blacks have been attributed to multiple factors. however, few articles have discussed spatial access to healthcare as a driver of disparities. the objective of this analysis was to evaluate the relationship between travel time to a healthcare provider and the likelihood of testing positive for 1 of 3 stis in a sample of adults living in public housing. methods:: one hundred and eight black adults in atlanta, ga ...201222249300
leukotriene b(4) receptors blt1 and blt2 are involved in interleukin-8 production in human neutrophils induced by trichomonas vaginalis-derived secretory products.trichomonas vaginalis is a flagellated protozoan parasite that causes human trichomoniasis. although t. vaginalis itself can secrete lipid mediator leukotriene (lt) b(4) leading to neutrophil activation, information regarding the signaling mechanism involved in neutrophil activation induced by t. vaginalis-secreted ltb(4) is limited. we investigated whether ltb(4) contained in the t. vaginalis-derived secretory products (tvsp) is closely involved in interleukin (il)-8 production in human neutrop ...201222215047
validation of novel fluorescence assays for the routine screening of drug susceptibilities of trichomonas vaginalis.objectives: to evaluate existing protocols, based on alamar blue (resazurin), for the routine determination of drug susceptibility in trichomonads, develop new ones and validate these by screening small antiprotozoal libraries. methods: the resazurin-based assay was evaluated by determining fluorescence development in trichomonas media with various cell densities after various intervals and in the presence of metronidazole. similar investigations were performed with the alternative fluorophores ...201222258922
antimicrobial resistance of abnormal vaginal discharges microorganisms in ouagadougou, burkina faso.to assess the prevalence of bacterial strains and fungal strains infecting the vaginal tract and test their sensitivity to antibiotics in women attending saint camille medical centre in ouagadougou.201223569916
[statement of the polish gynecological society expert group on the use of macmiror complex 500].the group of experts representing the polish gynecologic society has issued this statement based on the review of available literature on the potential benefits of the use of macmiror complex 500 in obstetrical and gynecologic practice. mixed vaginitis (mv) eg. the vaginal infection caused by at least two out of the triad of pathogens (fungi, bacteria and trichomonas vaginalis [tv]), constitutes the type of vaginitis which is underestimated as for its prevalence. mixed pathogens are responsible ...201223488303
opportunistic parasites in children with pneumococcal infection in greater cairo, egypt.vaccination remains the primary preventive strategy in the elderly against streptococcus pneumoniae and influenza infections. however, the effectiveness of vaccines decreases with increasing age and among patients. this study evaluated the opportunistic parasites infecting vaccinated and non-vaccinated pneumococcal children. the result showed that invasive children (4%) had bacternrmid, septicemid and pneumococcal but no meningitis while the non-invasive ones (86%) had community acquired freunon ...201223469649
the cytopathic effects of trichomonas vaginalis on fibroblast cell culture alone and with c. albicans and e. coli.in this study, the cytopathic effects of trichomonas vaginalis were investigated in l929 mouse fibroblast cell cultures (fcc) under different conditions: only parasite, or coexistence with candida albicans and escherichia coli.201223339937
fastannotator--an efficient transcript annotation web tool.recent developments in high-throughput sequencing (hts) technologies have made it feasible to sequence the complete transcriptomes of non-model organisms or metatranscriptomes from environmental samples. the challenge after generating hundreds of millions of sequences is to annotate these transcripts and classify the transcripts based on their putative functions. because many biological scientists lack the knowledge to install linux-based software packages or maintain databases used for transcri ...201223281853
[prevalence of sexually transmitted infections in pregnant women attending a public hospital in chile].the epidemiologic rates of gonorrhea have declined steadily in chile, while the incidence of infections with trichomonas vaginalis and chlamydia trachomatis is not well known.201223282493
sexually transmitted infections among hiv-infected heavy drinkers in st petersburg, russia.the objective of this study was to estimate the prevalence and identify correlates of four sexually transmitted infections (stis) among hiv-infected russians reporting heavy alcohol use and recent unprotected sex, we conducted a cross-sectional analysis of baseline data from the hermitage study. the primary outcome was any current sti, based on urine tests for neisseria gonorrhoeae, chlamydia trachomatis and trichomonas vaginalis and serological testing for infection with treponema pallidum. dat ...201223258823
comparative transcriptomic and proteomic analyses of trichomonas vaginalis following adherence to fibronectin.the morphological transformation of trichomonas vaginalis from an ellipsoid form in batch culture to an adherent amoeboid form results from the contact of parasites with vaginal epithelial cells and with immobilized fibronectin (fn), a basement membrane component. this suggests host signaling of the parasite. we applied integrated transcriptomic and proteomic approaches to investigate the molecular responses of t. vaginalis upon binding to fn. a transcriptome analysis was performed by using larg ...201222927047
prevalences of sexually transmitted infections in young adults and female sex workers in peru: a national population-based survey.we assessed prevalences of seven sexually transmitted infections (stis) in peru, stratified by risk behaviours, to help to define care and prevention priorities.201222878023
hiv and sti prevalence and determinants among male migrant workers in india.our objective was to estimate for the first time the prevalence and determinants of human immunodeficiency virus type 1 (hiv-1) and sexually transmitted infections (stis) among male migrants in india.201222952708
genital tract infections, bacterial vaginosis, hiv, and reproductive health issues among lima-based clandestine female sex workers.sociodemographic and behavioral characteristics of 212 peruvian female sex workers (fsws) were analyzed. the association between genital tract infections (gtis) and risk factors by multivariate analysis was evaluated. eighty-eight percent of fsws were diagnosed with at least one gti (hsv-2 80.1%, bv 44.8%, candidiasis 9.9%, syphilis seropositivity 9.4%, trichomonas vaginalis 2.4%, hiv seropositivity 2.4%). reported condom use with clients was nearly universal (98.3%), but infrequent with husband ...201222811592
the prevalence of vaginal microorganisms in pregnant women with preterm labor and preterm birth.to investigate the risk factors for vaginal infections and antimicrobial susceptibilities of vaginal microorganisms among women who experienced preterm birth (ptb), we compared the prevalence of vaginal microorganisms between women who experienced preterm labor (ptl) without preterm delivery and spontaneous ptb.201222563554
prevention of sexually transmitted infections in urban communities (peru preven): a multicomponent community-randomised controlled trial.previous community-randomised trials of interventions to control sexually transmitted infections (stis) have involved rural settings, were rarely multicomponent, and had varying results. we aimed to assess the effect of a multicomponent intervention on curable stis in urban young adults and female sex workers (fsws).201222341824
incentivising safe sex: a randomised trial of conditional cash transfers for hiv and sexually transmitted infection prevention in rural tanzania.the authors evaluated the use of conditional cash transfers as an hiv and sexually transmitted infection prevention strategy to incentivise safe sex.201222318666
determinants of per-coital-act hiv-1 infectivity among african hiv-1-serodiscordant couples.knowledge of factors that affect per-act infectivity of human immunodeficiency virus type 1 (hiv-1) is important for designing hiv-1 prevention interventions and for the mathematical modeling of the spread of hiv-1.201222241800
alternative 2-keto acid oxidoreductases in trichomonas vaginalis: artifact of histochemical staining.trichomonas vaginalis has been reported to possess alternative 2-keto acid oxidoreductases (kors). these enzymes preferentially used indolepyruvate in a reaction that resembled that of pyruvate:ferredoxin oxidoreductase (pfo). however, the kors did not reduce ferredoxin and remained active in metronidazole-resistant trichomonads lacking pfo. therefore, it was proposed that the kors may help trichomonads to survive in the presence of metronidazole. the kors were identified using activity staining ...201221963439
UDP-xylose and UDP-galactose synthesis in Trichomonas vaginalis.The presence of xylose and galactose residues in the structure of trichomonad lipoglycans was indicated by previous studies and the modification of any glycoconjugate with either monosaccharide requires the respective presence of the nucleotide sugars, UDP-xylose and UDP-galactose. Biosynthesis of UDP-xylose de novo is mediated by UDP-xylose synthase (UXS; UDP-glucuronic acid decarboxylase), which converts UDP-glucuronic acid to UDP-xylose, whereas UDP-galactose can be generated from UDP-glucose ...201222008417
Modified Field stain - rapid viability test for Trichomonas vaginalis.Aims: We previously reported that Modified Field Stain (MF) can be used as a rapid stain for diagnosis. In the present study we extend the observation to include the stain as an alternative method to assess viability of the cells. Methods and Results: Six isolates of Trichomonas vaginalis were used to assess the utility of the Modified Field stain as a rapid viability test for T. vaginalis and to compare with 0·4% Trypan Blue dye exclusion test in three conditions; normal in vitro culture grow ...201222040369
Involvement of mast cells in inflammation induced by Trichomonas vaginalis via crosstalk with vaginal epithelial cells.Vaginal epithelial cells (VECs) are thought to function as immune-responsive cells in trichomoniasis, and mast cells have been detected in vaginal smears and the vaginal wall in trichomoniasis. It therefore seemed possible that the VEC-trichomonad reaction might affect the activity of mast cells present in the lamina propria of the vaginal mucosa. In this study, we tested whether culture supernatants of VEC incubated with Trichomonas vaginalis (TCM) could stimulate mast cells. When VECs (MS74) w ...201221981317
Prevalence and Correlates of Mycoplasma genitalium Infection Among Female Sex Workers in Kampala, Uganda.Background. The importance of Mycoplasma genitalium in human immunodeficiency virus (HIV)-burdened sub-Saharan Africa is relatively unknown. We assessed the prevalence and explored determinants of this emerging sexually transmitted infection (STI) in high-risk women in Uganda. Methods. Endocervical swabs from 1025 female sex workers in Kampala were tested for Mycoplasma genitalium using a commercial Real-TM polymerase chain reaction assay. Factors associated with prevalent Mycoplasma genitalium, ...201222102734
ongoing sexually transmitted disease acquisition and risk-taking behavior among us hiv-infected patients in primary care: implications for prevention interventions.to better understand the factors associated with hiv- and sexually transmitted disease (std)-transmitting behavior among hiv-infected persons, we estimated std prevalence and incidence and associated risk factors among a diverse sample of hiv-infected patients in primary care.201222183836
transactional sexual relationships, sexually transmitted infection risk, and condom use among young black women in peri-urban areas of the western cape province of south africa.background: transactional sexual behavior has been demonstrated as an important factor underlying the hiv epidemic in sub-saharan africa. the aim of this study was to evaluate the relationship between having a history of transactional sexual relationships with condom use and sti risk. methods: participants completed a behavioral questionnaire in isixhosa and provided self-collected vaginal swabs which were tested for chlamydia trachomatis, neisseria gonorrhea, and trichomonas vaginalis. multinom ...201222265179
standard treatment regimens for nongonococcal urethritis have similar but declining cure rates: a randomized controlled trial.azithromycin or doxycycline is recommended for nongonococcal urethritis (ngu); recent evidence suggests their efficacy has declined. we compared azithromycin and doxycycline in men with ngu, hypothesizing that azithromycin was more effective than doxycycline.201223223595
female epidemiology of transcription-mediated amplification-based trichomonas vaginalis detection in a metropolitan setting with a high prevalence of sexually transmitted infection.recent literature has reported increased accuracy of trichomonas vaginalis transcription-mediated amplification (tma)-based analyte-specific reagent (asr) testing in female populations. a retrospective investigation assessed 7,277 female first-void urine, cervical, or vaginal specimens submitted from a high-prevalence sexually transmitted infection (sti) community to characterize prevalence of disease etiologies. the most common sti phenotype reflected detection of solely t. vaginalis (54.2% of ...201223015673
abnormal vaginal ph and mycoplasma genitalium infection.mycoplasma genitalium (mg) is a sexually transmitted pathogen linked to female morbidity, but testing for mg is not standardized. we aimed to determine which point-of-care (poc) vaginal tests could predict mg infection.201223158750
comparison of self-obtained penile-meatal swabs to urine for the detection of c. trachomatis, n. gonorrhoeae and t. vaginalis.self-obtained penile-meatal swabs and urine specimens have been used for chlamydia trachomatis (ct), neisseria gonorrhoeae (ng) and trichomonas vaginalis (tv) for outreach screening in men.201223093735
prevalence and risk factors for bacterial vaginosis and other vulvovaginitis in a population of sexually active adolescents from salvador, bahia, brazil.bacterial vaginosis, trichomoniasis, and genital candidiasis are considered the main etiologies of vulvovaginitis. few studies estimate the prevalence of vulvovaginitis among adolescents, especially in brazil. this study aimed to determine the prevalence and main risk factors associated with bacterial vaginosis and genital infection by c. albicans and trichomonas vaginalis among a group of adolescents from salvador, bahia, brazil. one hundred sexually active adolescents followed at an adolescent ...201223133306
the role of physical examination in diagnosing common causes of vaginitis: a prospective study.we evaluated agreement in diagnoses for bacterial vaginosis (bv), trichomonas vaginalis (tv) and vulvovaginal candidiasis (vvc) between clinicians examining the patient and performing diagnostic tests versus a clinician with access only to the patient's history and diagnostic findings from self-obtained vaginal swabs (sovs).201223019659
condom effectiveness against non-viral sexually transmitted infections: a prospective study using electronic daily diaries.to prospectively evaluate the protective value of consistent and correct use of latex condoms against the acquisition of chlamydia trachomatis, neisseria gonorrhoeae and trichomonas vaginalis.201223002192
modeling the impact of trichomonas vaginalis infection on hiv transmission in hiv-infected individuals in medical care.to assess factors associated with having a trichomonas vaginalis (tv) infection among persons receiving care for human immunodeficiency virus (hiv) and estimate the number of transmitted hiv infections attributable to tv.201222902662
prevalence of trichomonas vaginalis and coinfection with chlamydia trachomatis and neisseria gonorrhoeae in the united states as determined by the aptima trichomonas vaginalis nucleic acid amplification assay.our aim was to determine trichomonas vaginalis prevalence using the aptima trichomonas vaginalis assay (atv; gen-probe) and the prevalence of chlamydia trachomatis and neisseria gonorrhoeae coinfections in u.s. women undergoing screening for c. trachomatis/n. gonorrhoeae. discarded urogenital samples from 7,593 women (18 to 89 years old) undergoing c. trachomatis/n. gonorrhoeae screening using the aptima combo 2 assay (gen-probe) in various clinical settings were tested with atv. overall, t. vag ...201222622447
evaluation of association between vaginal infections and high-risk human papillomavirus types in female sex workers in spain.background. infection with and persistence of high-risk human papillomavirus (hr-hpv) are the strongest risk factors for cervical cancer. in addition, other genital microorganisms may also be involved in the progression of hpv-associated lesions. objetive. to evaluate the association of the vaginal microbiota (candida spp., trichomonas vaginalis, and bacterial vaginosis) with hr-hpv infection in spanish female sex workers (fsws). methods. this cross-sectional study involved 208 (fsws; age, 18-49 ...201222900198
use of nucleic acid amplification testing for diagnosis of anorectal sexually transmitted infections.nucleic acid amplification testing (naat) has become the preferred method to detect chlamydia trachomatis and neisseria gonorrhoeae, but no commercial tests are cleared by the u.s. food and drug administration for use with rectal swab samples. this study evaluated the performance of strand displacement amplification (sda) and transcription-mediated amplification (tma) to detect c. trachomatis and n. gonorrhoeae and to determine if tma could also detect mycoplasma genitalium and trichomonas vagin ...201222493338
chlamydia trachomatis, mycoplasma genitalium, and trichomonas vaginalis infections in men with nongonococcal urethritis: predictors and persistence after therapy.chlamydia trachomatis (ct), mycoplasma genitalium (mg), and trichomonas vaginalis (tv) are sexually transmitted infections (stis) associated with nongonococcal urethritis (ngu). we assessed their predictors and persistence after treatment.201222615318
can mailed swab samples be dry-shipped for the detection of chlamydia trachomatis, neisseria gonorrhoeae, and trichomonas vaginalis by nucleic acid amplification tests?dry-shipped and mailed vaginal swabs collected at home have been used in research studies for the detection of chlamydia trachomatis (ct), neisseria gonorrhoeae (gc), and trichomonas vaginalis (tv) by nucleic acid amplification tests (naats) in screening programs. a verification study was performed to compare the limit of detection of ct, gc, and tv on swabs that were dry-shipped to paired swabs that were wet-shipped in transport media through the us mail. the centers for disease control and pre ...201222578934
the effect of medical male circumcision on urogenital mycoplasma genitalium among men in kisumu, kenya.we determined the prevalence of urethral mycoplasma genitalium (mg) infection and whether infection was associated with circumcision status among men enrolled in the randomized trial of medical male circumcision to prevent hiv acquisition in kisumu, kenya.201222421693
high prevalence of hiv and sexually transmitted infections among male sex workers in abidjan, cote d'ivoire: need for services tailored to their needs.to assess condom use and prevalence of sexually transmitted infections (sti) and hiv among male sex workers (msw) in abidjan, côte d'ivoire.201222328644
Genetic characterization of three Cuban Trichomonas vaginalis virus. Phylogeny of Totiviridae family.Trichomonas vaginalis can be infected with double stranded RNA (dsRNA) viruses known as T. vaginalis virus (TVV). This viral infection may have important implications for trichomonal virulence and disease pathogenesis. In this study we identified and genetic characterized three strains of TVVs isolated from T. vaginalis in Cuba. The three new predicted sequences of capsid protein and RNA-dependent RNA polymerase amounted to the previously determined 20 TVV sequences and other 21 viruses of Totiv ...201222075038
three-year history of transcription-mediated amplification-based trichomonas vaginalis analyte-specific reagent testing in a subacute care patient population.a total of 7,899 specimens submitted for live clinical trichomonas vaginalis analyte-specific reagent (asr) screening from 2008 to 2010 were audited on the basis of patient gender, specimen source, molecular neisseria gonorrhoeae and chlamydia trachomatis results, and relative light unit (rlu) data yielded by t. vaginalis asr. only 1.4% of the screening was ordered by emergency department clinicians. the screening volume in 2010 was 126% higher than that in 2008. the proportions of annual female ...201121998416
The association between Trichomonas infection and incarceration in HIV-seropositive and at-risk HIV-seronegative women.The prevalence of Trichomonas vaginalis is higher among incarcerated women than in the general community. We sought to determine whether a history of incarceration itself was independently associated with trichomoniasis.201122082718
characterization of rnase mrp rna and novel snornas from giardia intestinalis and trichomonas vaginalis.eukaryotic cells possess a complex network of rna machineries which function in rna-processing and cellular regulation which includes transcription, translation, silencing, editing and epigenetic control. studies of model organisms have shown that many ncrnas of the rna-infrastructure are highly conserved, but little is known from non-model protists. in this study we have conducted a genome-scale survey of medium-length ncrnas from the protozoan parasites giardia intestinalis and trichomonas vag ...201122053856
the trichomonas vaginalis hydrogenosome proteome is highly reduced relative to mitochondria, yet complex compared with mitosomes.the human pathogen trichomonas vaginalis lacks conventional mitochondria and instead contains divergent mitochondrial-related organelles. these double-membrane bound organelles, called hydrogenosomes, produce molecular hydrogen. phylogenetic and biochemical analyses of hydrogenosomes indicate a common origin with mitochondria; however identification of hydrogenosomal proteins and studies on its metabolism have been limited. here we provide a detailed proteomic analysis of the t. vaginalis hydrog ...201122079833
Murine models of vaginal trichomonad infections.Trichomonas vaginalis and Tritrichomonas foetus cause common sexually transmitted infections in humans and cattle, respectively. Mouse models of trichomoniasis are important for pathogenic and therapeutic studies. Here, we compared murine genital infections with T. vaginalis and T. foetus. Persistent vaginal infection with T. foetus was established with 100 parasites but T. vaginalis infection required doses of 10(6), perhaps because of greater susceptibility to killing by mouse vaginal polymorp ...201121976570
identification of putative mirnas from the deep-branching unicellular flagellates.micrornas (mirnas) are a class of extensively studied rnai-associated small rnas that play a critical role in eukaryotic gene regulation. however, knowledge on the mirna and its regulation in unicellular eukaryotes is very limited. in order to obtain a better understanding on the origin of mirna regulation system, we used deep-sequencing technology to investigate the mirna expression pattern in four deep-branching unicellular flagellates: giardia lamblia, trichomonas vaginalis, tritrichomonas fo ...201122120185
trichomonas vaginalis pathobiology new insights from the genome sequence.the draft genome of the common sexually transmitted pathogen trichomonas vaginalis encodes one of the largest known proteome with 60,000 candidate proteins. this provides parasitologists and molecular cell biologists alike with exciting, yet challenging, opportunities to unravel the molecular features of the parasite's cellular systems and potentially the molecular basis of its pathobiology. here, recent investigations addressing selected aspects of the parasite's molecular cell biology are disc ...201122137583
antimicrobial activity, cytotoxicity, and phytochemical screening of voacanga globosa (blanco) merr. leaf extract (apocynaceae).to determine the antibacterial, antifungal, antiprotozoal, cytotoxic, and phytochemical properties of ethanol extracts of leaves of voacanga globosa (blanco) merr. (v. globosa).201122014741
comparison of dacron and nylon-flocked self-collected vaginal swabs and urine for the detection of trichomonas vaginalis using analyte-specific reagents in a transcription-mediated amplification assay.objectivesto compare self-collected vaginal swab (scvs) types and first-catch urine (fcu) to diagnose trichomonas vaginalis using analyte-specific reagents designed to be used in a transcription-mediated amplification assay.methodsa total of 241 women (group a) collected a fcu and a scvs using a dacron swab (aptima collection kit). a second group of 289 women (group b) collected two scvs using one dacron swab and one nylon-flocked swab.resultsof 75 young women (street youth) determined to be inf ...201122166474
a machine-learning approach to identify hydrogenosomal proteins in trichomonas vaginalis.the protozoan parasite trichomonas vaginalis is the causative agent of trichomoniasis, the most widespread non-viral sexually transmitted disease in humans. it possesses hydrogenosomes - anaerobic mitochondria that generate h(2), co(2), and acetate from pyruvate while converting adp to atp via substrate level phosphorylation. t. vaginalis hydrogenosomes lack a genome and translation machinery, hence they import all their proteins from the cytosol, but to date only 30 imported proteins have been ...201122140228
chemical structure of trichomonas vaginalis surface lipoglycan: a role for short galactose (β1-4/3) n-acetylglucosamine repeats in host cell interaction.the extracellular parasite trichomonas vaginalis contains a surface glycoconjugate that appears to mediate parasite-host cell interaction via binding to human galectin-1. this glycoconjugate also elicits cytokine production from human vaginal epithelial cells, implicating its role in modulation of host immune responses. we have analyzed the structure of this glycoconjugate, previously described to contain the sugars rhamnose (rha), n-acetylglucosamine (glcnac), galactose (gal), xylose (xyl), n-a ...201121900246
molecular testing for trichomonas vaginalis in women: results from a prospective u.s. clinical trial.trichomoniasis is a common sexually transmitted disease associated with preterm birth, low birth weight, and increased susceptibility to infection with other pathogenic sexually transmitted microorganisms. nucleic acid amplification tests for trichomonas vaginalis have improved sensitivity for detecting infected individuals compared to existing culture-based methods. this prospective, multicenter u.s. clinical trial evaluated the performance of the automated aptima t. vaginalis assay for detecti ...201121940475
ultrastructural changes during asexual multiple reproduction in trichomonas vaginalis.trichomonas vaginalis, a flagellated protozoan parasite, is commonly found in the genitourinary tract of humans. its mode of reproduction has always been reported to be binary fission. the high parasite numbers seen in a relatively short period in in vitro cultures led us to believe that there must be other modes of reproduction. the present study for the first time provides transformational evidence at the ultrastructural level seen in tropohozoites of t. vaginalis undergoing a multiple asexual ...201122076052
first report of anti-trichomonas vaginalis activity of the medicinal plant polygala decumbens from the brazilian semi-arid region, caatinga.trichomonosis, caused by the flagellate protozoan trichomonas vaginalis, is the most common non-viral sexually transmitted disease worldwide. actually, the infection treatment is based on 5-nitroimidazole drugs. however, an emergent number of resistant isolates makes important the search for new therapeutic arsenal. in this sense, the investigation of plants and their metabolites is an interesting approach. in the present study, the anti-t. vaginalis activity of 44 aqueous extracts from 23 caati ...201122205352
[which is the best empirical treatment in patients with urethritis?].to know the best empirical treatment of urethritis in patients at the city center of madrid.201121947096
antiprotozoal activity of proton-pump inhibitors.parasitic diseases are still a major health problem in developing countries. in our effort to find new antiparasitic agents, in this letter we report the in vitro antiprotozoal activity of omeprazole, lansoprazole, rabeprazole and pantoprazole against trichomonas vaginalis, giardia intestinalis and entamoeba histolytica. molecular modeling studies were an important tool to highlight the potential antiprotozoal activity of these drugs. experimental evaluations revealed a strong activity for all c ...201122047694
[incidence in sivas of trichomonas vaginalis in patients with vaginitis].objective: trichomonas vaginalis infection is a common disease among women and an important public health problem. the present study is performed to determine the prevalence of t. vaginalis among the 258 women admitted to sivas state hospital, gynecology department with a variety of symptoms and vaginitis. the ages of patients ranges from 17 to 80 years. methods: during the gynecologic examination two samples were taken from the vagina fornix and the first one was placed ...201122203503
sexually transmitted infections and risk behaviors among african american women who have sex with women: does sex with men make a difference?we sought to determine the prevalence of infection with chlamydia trachomatis, neisseria gonorrhoeae, trichomonas vaginalis, mycoplasma genitalium, syphilis, and hiv among african american women who have sex with women (aawsw), and compare sociodemographics, sexual risk behavior characteristics, and sti diagnoses among women reporting sex exclusively with women (exclusive aawsw) to women reporting sex with both women and men (aawswm) during the past 12 months.201122082722
cytology and clinical spectrum of sexually transmitted infections in lebanese women as revealed by pap smear: a cross-sectional study from 2002-2006.to study the cytology profile of cervical smears and the respective prevalence and incidence of certain cervico-vaginal infections detectable by routine pap smear cytology in lebanese women from 2002 to 2006. pap smear cytology results were compiled from the archives of the institut national de pathologie for the period extending from 2002 until 2006. this study covered 118,230 cervical specimens obtained from lebanese women attending clinics and hospitals in all the five districts of lebanon; p ...201122023770
screening and bioguided fractionation of amaryllidaceae species with anti-trichomonas vaginalis activity.the amaryllidaceae family is known by its ornamental and medicinal value and has attracted considerable attention due to the content of alkaloids of its species, which showed interesting biological properties. the present study evaluated the anti-trichomonas vaginalis activity of eighteen extracts (12.5 to 0.19 mg/ml) and six isolated alkaloids (125 to 1.9 µg/ml) from amaryllidaceae species. the alkaloids diminished the trophozoites viability (from 15 to 40 %). the extracts from hippeastrum brev ...201121308614
lycorine induces cell death in the amitochondriate parasite, trichomonas vaginalis, via an alternative non-apoptotic death pathway.in this study, the mechanism of action of the pro-apoptotic alkaloid lycorine on an amitochondriate cell, the parasite trichomonas vaginalis, was investigated. the cytotoxicity of lycorine against t. vaginalis was studied from 2.5 to 1000µm and several important ultrastructural alterations were observed by electron microscopy. lycorine arrested the t. vaginalis cell cycle, although no hallmarks of apoptosis, such as apoptotic bodies, were observed. consequently, the underlying mechanism of actio ...201121324496
antimicrobial topical agents used in the vagina.vaginally applied antimicrobial agents are widely used in the vagina in women with lower genital tract infections. an 'antimicrobial' is a general term that refers to a group of drugs that are effective against bacteria, fungi, viruses and protozoa. topical treatments can be prescribed for a wide variety of vaginal infections. many bacterial infections, such as bacterial vaginosis, desquamative inflammatory vaginitis or, as some european authors call it, aerobic vaginitis as well as infection wi ...201121325838
clinical isolates of trichomonas vaginalis concurrently infected by strains of up to four trichomonasvirus species (family totiviridae).trichomonas vaginalis, which causes the most common nonviral sexually transmitted disease worldwide, is itself commonly infected by nonsegmented double-stranded rna (dsrna) viruses from the genus trichomonasvirus, family totiviridae. to date, cdna sequences of one or more strains of each of three trichomonasvirus species have been reported, and gel electrophoresis showing several different dsrna molecules obtained from a few t. vaginalis isolates has suggested that more than one virus strain mig ...201121345965
fermentation enzymes of giardia intestinalis, pyruvate:ferredoxin oxidoreductase and hydrogenase, do not localize to its mitosomes.it is becoming increasingly clear that the so-called remnant organelles of microaerophilic unicellular eukaryotes, hydrogenosomes and mitosomes, are significantly reduced versions of mitochondria. they normally lack most of the classic mitochondrial attributes, such as an electron transport chain and a genome. while hydrogenosomes generate energy by substrate-level phosphorylation along a hydrogen-producing fermentation pathway, involving iron-sulfur-cluster-containing enzymes pyruvate : ferredo ...201121349979
comparison of affirm vpiii and papanicolaou tests in the detection of infectious vaginitis.to compare the affirm vpiii molecular test (becton dickinson, burlington, nc) with morphologic identification used in routine papanicolaou (pap) test screening in the detection and identification of candida species, trichomonas vaginalis, and gardnerella vaginalis, we identified 431 cases with a concomitant pap test and affirm vpiii assay performed from the archives of a large academic institution. the study population consisted of women ranging in age from 17 to 79 years (mean and median ages, ...201121350100
the prevalence of urogenital micro-organisms detected by a multiplex pcr-reverse line blot assay in women attending three sexual health clinics in sydney, australia.this study used a previously described multiplex pcr-based reverse line blot (mpcr/rlb) assay to assess the prevalence and distribution of 14 urogenital pathogens or putative pathogens, namely neisseria gonorrhoeae, chlamydia trachomatis, mycoplasma genitalium, mycoplasma hominis, trichomonas vaginalis, gardnerella vaginalis, ureaplasma parvum, ureaplasma urealyticum, neisseria meningitidis, streptococcus pneumoniae, haemophilus influenzae, herpes simplex virus types 1 and 2, and human adenoviru ...201121415210
genital chlamydia, genital herpes, trichomonas vaginalis and gonorrhea prevalence, and risk factors among nearly 70,000 randomly selected women in 4 nordic countries.background:: the aim of this study was to assess the prevalence of women reporting ever having genital chlamydia, genital herpes, trichomonas vaginalis, and gonorrhea, and to identify factors associated with each of these sexually transmitted infections (stis). methods:: the study was based on a large cross-sectional survey conducted in 2004-2005 among randomly sampled women (18-45 years) from the computerized population registries in denmark, iceland, norway, and sweden. a total of 69,567 women ...201121844702
involvement of purinergic signaling on nitric oxide production by neutrophils stimulated with trichomonas vaginalis.trichomonas vaginalis is a parasite from the human urogenital tract that causes trichomonosis, the most prevalent non-viral sexually transmitted disease. the neutrophil infiltration has been considered to be primarily responsible for cytological changes observed at infection site, and the chemoattractants can play an important role in this leukocytic recruitment. nitric oxide (no) is one of the most widespread mediator compounds, and it is implicated in modulation of immunological mechanisms. ex ...201121833696
novel trichomonacidal spermicides.metronidazole, the u.s. food and drug administration-approved drug against trichomoniasis, is nonspermicidal and thus cannot offer pregnancy protection when used vaginally. furthermore, increasing resistance of trichomonas vaginalis to 5-nitro-imidazoles is a cause for serious concern. on the other hand, the vaginal spermicide nonoxynol-9 (n-9) does not protect against sexually transmitted diseases and hiv in clinical situations but may in fact increase their incidence due to its nonspecific, su ...201121709091
phylogeny of parasitic parabasalia and free-living relatives inferred from conventional markers vs. rpb1, a single-copy gene.parabasalia are single-celled eukaryotes (protists) that are mainly comprised of endosymbionts of termites and wood roaches, intestinal commensals, human or veterinary parasites, and free-living species. phylogenetic comparisons of parabasalids are typically based upon morphological characters and 18s ribosomal rna gene sequence data (rdna), while biochemical or molecular studies of parabasalids are limited to a few axenically cultivable parasites. these previous analyses and other studies based ...201121695260
trichomonas vaginalis as a rare cause of male factor infertility at a hospital in east anatolia.trichomonas vaginalis is known as an important cause of sexually transmitted infection in developing countries. the prevalence and spectrum of trichomonasis in men are less characterised. we analysed the presence of t.-ávaginalis in 80 infertile men using wet mount microscopy, giemsa staining, culture and pcr methods. we found 2.5% positivity for t.-ávaginalis using pcr method. wet mount microscopy was ineffective. giemsa staining and culture tests were positive only in one patient. both of our ...201121729126
leukotriene b(4) receptor blt-mediated phosphorylation of nf-+¦b and creb is involved in il-8 production in human mast cells induced by trichomonas vaginalis-derived secretory products.trichomonas vaginalis is a protozoan parasite that causes acute tissue inflammation in vaginal trichomoniasis. in this study, we investigated the signaling mechanisms through which t. vaginalis-derived secretory products (tvsp) induce chemokine il-8 production in human mast cells. stimulation with tvsp induced up-regulation of il-8 protein secretion in hmc-1 cells. in addition, tvsp induced phosphorylation of transcription factors nf-+¦b and creb in hmc-1 cells. pretreatment of tvsp with lipase, ...201121824526
male accessory gland infection and sperm parameters (review).male accessory gland infection (magi) has been identified among those diagnostic categories which have a negative impact on the reproductive function and fertility in males (rowe et-áal., world health organization manual for the standardised investigation and diagnosis of the infertile couple, cambridge university press, cambridge, 1993). magi is a hypernym which groups the following different clinical categories: prostatitis, prostate-vesiculitis and prostate-vesiculo-epididymitis. some of the ...201121696400
trichomonas vaginalis infection in women who submit self-obtained vaginal samples after internet recruitment.: submission of self-obtained vaginal samples (sovs) collected at home could remove barriers that women face in getting tested for sexually transmitted infections (stis). internet recruitment of sovs is highly acceptable.201121844738
activity of cuban propolis extracts on leishmania amazonensis and trichomonas vaginalis.in this paper we analyzed the antiprotozoal effects of eighteen cuban propolis extracts (brown, red and yellow type) collected in different geographic areas, using leishmania amazonensis (as a model of intracellular protozoa) and trichomonas vaginalis (as a model of extracellular protozoa). all evaluated propolis extracts caused inhibitory effect on intracellular amastigotes of l. amazonensis. however, cytotoxicity on peritoneal macrophages from balb/c mice was observed. only five samples decrea ...201121834236
Refresh
firstprev
12345678910...
nextlast
Displaying items 801 - 900 of 4838