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studies with lectins on the surface carbohydrate structures of mycoplasma membranes.the surface carbohydrate structures on the cell membranes of various mycoplasma species have been investigated by using lectins, which are sugar-specific proteins. carbohydrate structures presumably bound to glycolipids, with both galactose and glucose units, were found to be exposed on the surface of mycoplasma pneumoniae and its temperature-sensitive mutants, m. mycoides var. mycoides and capri, m. pulmonis, m. gallinarum, and m. gallisepticum. lipid-bound glucose was found on m. neurolyticum. ...19744138526
an electron-microscopic examination of certain bovine mycoplasmas stained with ruthenium red and the demonstration of a capsule on mycoplasma dispar. 19744139238
use of the avidin-biotin complex for specific staining of biological membranes in electron microscopy.to expand the electron microscopist's options in localization and visualization, a new and general staining technique has been tested. the avidin-biotin complex serves as a coupling between the electron-dense marker, ferritin, and points of interest in biological samples. when specific cellular components are tagged with biotin, those components may be visualized with ferritin-linked avidin. because of the remarkably strong affinity of avidin and biotin (characterized by an association constant ...19744139715
incidence and significance of mycoplasmas in sick cats. 19744206697
immunological analysis of glycolipids and lipopolysaccharides derived from various mycoplasmas.the lipids and lipopolysaccharides of five mycoplasmas were examined for complement-fixing activity to antimembrane rabbit sera. total glycolipid fractions and the aqueous phenol fractions (lipopolysaccharides) from the membranes of acholeplasma laidlawii, a. modicum, a. axanthum, and mycoplasma neurolyticum exhibited significant antigenic activity. the glycolipids and phosphoglycolipids from thermoplasma acidophilum were either anticomplementary or did not react due to their extreme hydrophobic ...19744215761
development of high resolution pyrolysis-gas chromatography for the identification of microorganisms. 19744217802
polyene antibiotic-sterol interactions in membranes of acholesplasma laidlawii cells and lecithin liposomes. ii. temperature dependence of the polyene antibiotic-sterol complex formation. 19744277610
regulation of membrane lipid fluidity in acholeplasma laidlawii: effect of carotenoid pigment content. 19744366832
control of membrane lipid fluidity in acholeplasma laidlawii. 19744368536
lipid and temperature dependence of membrane-bound atpase activity of acholeplasma laidlawii. 19744371925
differential effect of phenethyl alcohol on mycoplasmas and enveloped viruses.mycoplasmas are totally inactivated by a 20-min treatment with 1% phenethyl alcohol, whereas suspensions of enveloped viruses resist the same treatment. this treatment has proved successful in eliminating mycoplasma contamination of a virus suspension.19744372415
[sensitivity range of gram-negative microorganisms and mycoplasms to minocycline in vitro]. 19744373015
induction of interferon in mice by mycoplasmas.interferon was induced in mice after intraperitoneal inoculation with four different mycoplasmas. peak levels of between 100 and 300 u of interferon per ml were attained by 6 h postinfection with each of the mycoplasmas except mycoplasma arthritidis, which induced higher titers (400 to 11,800 u/ml) by this time. a fifth mycoplasma, m. pulmonis, induced interferon inconsistently and at a later (72 to 96 h) time. mycoplasmatales virus mvl51 and sterile mycoplasmal broth did not stimulate interfero ...19744373395
cholesterol in mycoplasma membranes. ii. components of acholeplasma laidlawii cell membranes responsible for cholesterol binding. 19744378339
isolation, solubilization, and reconstitution of mycoplasma membranes. 19744378340
characterization of the mycoplasma membrane proteins. iv. disposition of proteins in the membrane. 19744407052
[maltose utilization by acholeplasma laidlawii cells]. 19744407457
correlation of cholesterol to phospholipid content in membranes of growing mycoplasmas. 19744426401
acholeplasma laidlawii and pasteurella multocida isolated from the pneumonic lung of a lynx. 19744436927
[mixed infection of mice of line bal/b/cde with rauscher virus and mycoplasma laidlawii]. 19744446553
the effect of membrane-lipid phase transitions on membrane structure and on the growth of acholeplasma laidlawii b. 19744461849
reconstitution of mycoplasma membranes. 19744461850
requirements of acholeplasma laidlawii a, strain la 1, for nucleic acid precursors. 19744471597
aminopeptidase activity of ureaplasma urealyticum. 19744533227
radioprotection of acholeplasma laidlawii b by cysteine. 19744545833
polyene antibiotic-sterol interactions in membranes of acholeplasma laidlawii cells and lecithin liposomes. i. specificity of the membrane permeability changes induced by the polyene antibiotics. 19744546884
polyene antibiotic-sterol interactions in membranes of acholeplasma laidlawii cells and lecithin liposomes. 3. molecular structure of the polyene antibiotic-cholesterol complexes. 19744546885
protection of acholeplasma laidlawii b by superoxide dismutase. 19744548416
[identification of mycoplasma and bacterial l forms by polyacrylamide gel electrophoresis]. 19744598211
isolation of acholeplasma laidlawii from commercial, serum-free tissue culture medium and studies on its survival and detection.this report documents the first isolation of acholeplasma laidlawii from a commercial lot of serum-free dulbecco basal medium. experimental studies demonstrated survival of the organism for at least 1 year depending on the concentration of the contaminating organism as well as ph and temperature of storage of the serum-free medium. a comparison of isolation methods showed that concentration by filtration through 220-nm membrane filters and testing the filters for mycoplasma recovery, especially ...19744598432
epidemiologic observations on mycoplasma mastitis. 19744602678
[isolation of membrane preparations from acholeplasma laidlawii cells and their transport characteristics]. 19744603647
effect of mycoplasma dispar, mycoplasma bovirhinis, acholeplasma laidlawii and t-mycoplasmas on explant cultures of bovine trachea. 19744604399
mycoplasma viruses: isolation, physicochemical, and biological properties. 19744604802
differential scanning calorimetry. 19744614000
aspiculamycin, a new cytosine nucleoside antibiotic. iv. antimycoplasma activity of aspiculamycin in vitro and in vivo. 19744616935
proceedings: fluorescent labelling with 5-dimethyl-aminonaphthalene-1-sulfonyl chloride of membrane surface proteins of gram-negative bacteria. 19744618247
a whole cell mounting technique for detecting acholeplasma laidlawii a on the surface of cultured cells. 19744620358
incorporation of 3h-uridine and 3h-uracil into rna: a simple technique for the detection of mycoplasma contamination of cultured cells. 19744817725
replacement of the octadecenoic acid growth-requirement for acholeplasma laidlawii a by cis-9,10-methylenehexadecanoic acid, a cyclopropane fatty acid. 19744820352
intracellular replication of mycoplasmavirus mvl51.the intracellular replication of mvl51, a group l1 mycoplasmavirus, was investigated. the single-stranded parental dna was found to enter the cell and become converted to double-stranded dna. this replicated to yield additional double-stranded dna molecules. the parental viral dna was found to leave the replication complex and become associated with large molecular weight dna not involved with viral replication. progeny viral dna formed from the double-stranded dna and an intracellular accumulat ...19744821487
radiobiological properties of acholeplasma laidlawii b. 19744822050
interaction of mycoplasma with viruses. i. primary adsorption of virus is ionic in mechanism.we have studied the adsorption of the gourlay acholeplasma virus mvl-1 to the host cell acholeplasma laidlawii ja-1. successful adsorption depends primarily on some unknown action of the serum factor in the medium, but evaluation of various physical parameters indicates clearly that given this factor the kinetics is pseudo first order (k = 3 x 10(-9) cm(3)/min) and the mechanism ionic. chiefly important are the ionic strength of the cation and ph (optimal na(+) = 0.08 m, ph = 6). the system seem ...19744824711
the effect of alterations in the physical state of the membrane lipids on the ability of acholeplasma laidlawii b to grow at various temperatures. 19744830862
cholesterol in mycoplasma membranes. i. kinetics and equilibrium studies of cholesterol uptake by the cell membrane of acholeplasma laidlawii. 19744841672
effect of fatty acyl chain length on some structural and functional parameters of acholeplasma membranes. 19744844283
isolation of acholeplasma laidlawii from guinea-pigs. 19744852593
a simplified method for the detection of mycoplasma. 19744858125
spiroplasmas are the causal agents of citrus little-leaf disease.a spiroplasma isolated from citrus with little-leaf disease was grown in a cell-free medium and injected into leafhoppers (euscelis plebejus). injected leafhoppers, but not those fed on infected plants, transmitted the spiroplasma to white clover (trifolium repens cv. s100) and sweet orange (citrus sinensis cv. valencia). infected clover plants were severely stunted; infected sweet orange plants showed typical symptoms of citrus little-leaf disease. the spiroplasma was detected in clover and swe ...197419280788
divalent cations in native and reaggregated mycoplasma membranes.the mg(2+) content of membranes of several mycoplasma and acholeplasma species varied between 0.88 and 1.98 mug of mg(2+) per mg of protein, depending on the species and on growth conditions. ca(2+) could be detected only when it was added to the growth medium. the mg(2+) content of isolated a. laidlawii membranes could be increased almost threefold by dialysis against 20 mm mg(2+), whereas aggregated a. laidlawii membranes contained about six to eight times more mg(2+) per mg of protein than th ...19734632319
freeze-fractured acholeplasma laidlawii membranes: nature of particles observed.freeze-fracture of acholeplasma laidlawii membranes from cells incubated in the presence of puromycin or omission of amino acids reveals a decrease in the number of particles between 50 and 100 angstroms in the hydrophobic fracture plane, which strongly suggests that these particles are protein. additional evidence indicates that they may be involved in substrate transport.19734682132
[antigenic relationship of saprophytic and plant mycoplasmas]. 19734683168
weak association of glucosamine-containing polymer with the acholeplasma laidlawii membrane. 19734684605
chemical composition and serology of mycoplasma pneumoniae lipids. 19734689627
freeze-etch electron microscopy of erythrocytes, acholeplasma laidlawii cells and liposomal membranes after the action of filipin and amphotericin b. 19734690870
[carbohydrate transport in mycoplasma laidlawii cells]. 19734696486
molecular model for phase transition in biological membranes. 19734705755
circular dichroism analysis of native and reaggregated mycoplasma membranes. 19734706839
on the phosphorylation of sugars in acholeplasma laidlawii. 19734709990
the effect of the polar headgroup on the lipid-cholesterol interaction: a monolayer and differential scanning calorimetry study. 19734711186
properties of a fructose-1,6-diphosphate-activated lactate dehydrogenase from acholeplasma laidlawii type a.acholeplasma laidlawii a possesses a nicotinamide adenine dinucleotide (nad)-dependent l(+)-lactate dehydrogenase (ldh) which is activated specifically by low concentrations of fructose-1, 6-diphosphate (fdp). studies with partially purified enzyme show that the kinetic response to fdp is hyperbolic. the enzyme is inhibited by inorganic phosphate, adenosine triphosphate, and high concentrations of reduced nad (nadh). low activity is demonstrable in the absence of fdp at ph 6.0 to 7.2, but fdp is ...19734712565
the effect of alterations in fatty acid composition and cholesterol content on the nonelectrolyte permeability of acholeplasma laidlawii b cells and derived liposomes. 19734719142
polyacrylamide gel electrophoresis of mycoplasma proteins in sodium dodecyl sulphate. 19734723070
further studies on the morphology and composition of mycoplasmatales virus-laidlawii 2. 19734735309
isolation of mycoplasmatales virus-laidlawii 3, a new virus infecting acholeplasma laidlawii. 19734736684
studies of sugar transport in acholeplasma laidlawii. 19734772677
selective simultaneous proton magnetic resonance labelling of hydrophilic and hydrophobic regions of phospholipid vesicles. effect of polar and apolar reagents. 19734795847
spiroplasmas and acholeplasmas: multiplication in insects.the helical wall-free microorganism, spiroplasma citri, which is associated with citrus stubborn, a disease with no known vector, multiplied in the leafhopper vector of corn stunt but multiplied to higher titer in the vector of aster yellows and decreased the longevity of that insect. acholeplasma laidlawii and a. granularum also multiplied in both leafhoppers.19734796196
isolation and purification of tdna from mycoplasma. 19734803254
nmr studies of sodium and potassium in various biological tissues. 19734513156
fusion of phospholipid vesicles with viable acholeplasma laidlawii.when vesicles of dipalmitoyl phosphatidylcholine produced by sonication are mixed with acholeplasma laidlawii in neutral buffer, a phenomenon occurs that is most readily explained in terms of fusion of lipid vesicle membrane with the mycoplasma cytoplasmic membrane. the mycoplasma can readily accumulate large quantities of the foreign lipid without loss of viability. the added lipids do not remain in patches but diffuse laterally throughout the mycoplasma membrane.19734515622
genome size and evolution. 19734565043
workshop on the mycoplasmatales as agents of disease. session i. biology of the mycoplasmatales. summary of discussion. 19734570125
acyl carrier protein in mycoplasmas.acyl carrier protein (acp) activity was determined by the malonyl-coenzyme a-co(2) exchange reaction. it was highest in acholeplasma laidlawii, lower in a. granularum, and lowest in a. axanthum. the sterol-requiring mycoplasma species examined showed little or negligible acp activity. a. laidlawii was capable of utilizing pantetheine or coenzyme a but not beta-alanine as precursor for acp synthesis. its acp could thus be labeled by growing the organisms with radioactive coenzyme a. the acp of a. ...19734570599
membrane lipids of mycoplasma hominis.essentially all of the lipids of mycoplasma hominis (200 mug/mg of cell protein) were found to be located in the cell membrane. over one-half were neutral lipids incorporated from the growth medium and consisting of 43% free cholesterol, 19% esterified cholesterol, 23% triglycerides, 10% free fatty acids, and small amounts of di- and monoglycerides. the polar lipids accounting for about 40% of the total were synthesized by the organisms. phosphatidylglycerol was the predominant lipid of this fra ...19734347923
virus-like particles associated with the mycoplasmas of clover phyllody in the plant and in the insect vector. 19734348972
dilatometry biological membranes. 19734350105
effect of proteins on the motion of spin-labeled fatty acids in mycoplasma membranes. 19734350558
the effect of different fatty acid and sterol composition on the erythritol flux through the cell membrane of acholeplasma laidlawii. 19734352491
binding of proteins to mycoplasma membranes. 19734354127
reaction of mice on infection with mycoplasma and leukosis viruses. 19734354218
mycoplasma-associated induction of interferon in ovine leukocytes.a mycoplasmal species, acholeplasma laidlawii, isolated as a contaminant from a fetal lamb kidney cell line, was shown to be associated with the induction of interferon in cultures of ovine peripheral blood leukocytes. broth cultures of the mycoplasma induced between 20 and 230 u of interferon per ml in leukocytes from two adult ewes. the amount of interferon produced correlated with the inoculum size of mycoplasma. interferon production was associated with replication of the mycoplasma in the l ...19734356073
lipid- and temperature-dependent structural changes in acholeplasma laidlawii cell membranes. 19734357019
influence of fatty acid and sterol composition on the lipid phase transition and activity of membrane-bound enzymes in acholeplasma laidlawii. 19734149248
[a comparative analysis of the behavior of l forms of bacteria and mycoplasma in cell cultures]. 19734149907
some differences between two viruses infecting acholeplasma laidlawii. 19734120326
immunologically cross-reacting proteins in cell walls of many bacteria.antibodies to a protein present in the cell envelope of hydrogenomonas facilis agglutinate many gram-negative bacteria and a few gram-positive bacteria. immunodiffusion studies of extracts from the various organisms tested indicate the presence of components sharing antigenic determinants with the cell envelope protein in all bacteria which can be agglutinated and a few that cannot. cross-reacting components were not detected in extracts of mycoplasma laidlawii, anacystis nidulans, and several e ...19734120606
infection of acholeplasma laidlawii by mvl51 virus. 19734125688
[electron microscopy studies of cell cultures: demonstration of a mixed infection by egtved virus of the rainbow trout and mycoplasma (acholeplasma laidlawii) (author's transl)]. 19734131319
[morphology and ultrastructure of mycoplasma (acholeplasma) laidlawii cells]. 19734133913
characterization of mycoplasmatales virus dna. 19734573075
are cell membranes fluid? 19734574849
cultivation of mycoplasmas on cellulose ester substrates.the ability of mycoplasmas to grow on cellulose ester substrates was evaluated. mycoplasma pneumoniae, m. hominis, m. arthritidis, m. gallisepticum, and acholeplasma laidlawii grew on millipore (mixed cellulose ester) filters and sepraphore iii (cellulose polyacetate) membranes.19734584581
selective membrane toxicity of the polyene antibiotics: studies on natural membranes.the effect of polyene antibiotics on candida albicans, human erythrocytes, and acholeplasma laidlawii was studied. the results sustain the observations made with lecithin-sterol liposomes. the distribution of double bonds in the membrane sterol nucleus appears to be of major importance in conferring polyene susceptibility; those sterols with the ergosterol nucleus are far more effective than those with a nucleus similar to cholesterol. different polyenes vary in their membrane selectivity. the c ...19734586145
protein-lipid interactions. 19724592575
recent studies using nuclear magnetic resonance spectroscopy of lipids and biological membranes. 19724580642
the effect of cholesterol and epicholesterol incorporation on the permeability and on the phase transition of intact acholeplasma laidlawii cell membranes and derived liposomes. 19725011001
studies on the biological properties of polyene antibiotics. evidence for the direct interaction of filipin with cholesterol. 19725012767
polyacrylamide-gel electrophoresis of cell proteins of mycoplasma isolated from cattle and horses. 19725017869
distribution of mycoplasmas in the non-pneumonic bovine respiratory tract. 19725018036
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