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vancomycin-resistant enterococcus faecium (vre) bacteremia in infective endocarditis successfully treated with combination daptomycin and tigecycline. 201121803704
antimicrobial activity of daptomycin tested against gram-positive strains collected in european hospitals: results from 7 years of resistance surveillance (2003-2009).daptomycin is a cyclic lipopeptide approved by the european medicines agency (emea) for the treatment of complicated skin and soft tissue infections (cssti) and staphylococcus aureus bacteremia and endocarditis. we evaluated the in vitro activity of daptomycin and comparators tested against clinical isolates from european hospitals over a 7-year period (2003-2009). a total of 36,769 consecutive isolates were collected in 34 medical centers located in 13 european countries, turkey and israel. the ...201121803696
anti-hiv siamycin i directly inhibits autophosphorylation activity of the bacterial fsrc quorum sensor and other atp-dependent enzyme activities.siamycin i disrupts growth and quorum sensing in enterococcus faecalis. using purified intact protein, we demonstrate here that quorum membrane sensor kinase fsrc is a direct target of siamycin i, reducing pheromone-stimulated autophosphorylation activity by up to 91%. inhibition was non-competitive with atp as substrate. other atp-binding enzymes were also inhibited, including nine other membrane sensor kinases of e. faecalis, rhodobacter sphaeroides prrb, porcine na(+)-dependent atpase and the ...201121803040
influence of a probiotic soy product on fecal microbiota and its association with cardiovascular risk factors in an animal model.abstract: background: previous work showed that daily ingestion of an aqueous soy extract fermented with enterococcus faecium crl 183 and lactobacillus helveticus 416, supplemented or not with isoflavones, reduced the total cholesterol and non-hdl-cholesterol levels, increased the high-density lipoprotein (hdl) concentration and inhibited the raising of autoantibody against oxidized low-density lipoprotein (ox-ldl ab) and the development of atherosclerotic lesions. objective: the aim of this stu ...201121801422
the oral health of critically ill children: an observational cohort study.aims and objectives. this study will describe the oral health status of critically ill children over time spent in the paediatric intensive care unit, examine influences on the development of poor oral health and explore the relationship between dysfunctional oral health and healthcare-associated infections. background. the treatment modalities used to support children experiencing critical illness and the progression of critical illness may result in dysfunction in the oral cavity. in adults, ...201121801254
a reference map of the membrane proteome of enterococcus faecalis.enterococcus faecalis is a gram-positive bacterium that is part of the indigenous microbiotica of humans and animals as well as an opportunistic pathogen. in this study we have fractionated the membrane proteome of e. faecalis and identified many of its constituents by mass spectrometry. we present bn-/sds-page reference maps that contain 102 proteins. these proteins are important for cellular homeostasis, virulence, and antibiotic intervention. intriguingly, many proteins with no known function ...201121800426
initial microbial spectrum in severe secondary peritonitis and relevance for treatment.this study aims to determine whether abdominal microbial profiles in early severe secondary peritonitis are associated with ongoing infection or death. the study is performed within a randomized study comparing two surgical treatment strategies in patients with severe secondary peritonitis (n?=?229). the microbial profiles of cultures retrieved from initial emergency laparotomy were tested with logistic regression analysis for association with 'ongoing infection needing relaparotomy' and in-hosp ...201121800218
effects of electron beam irradiation on the variability in survivor number and duration of lag phase of four food-borne organisms.the effect of electron beam irradiation on microbial inactivation and duration of lag time of individual surviving cells of listeria innocua, enterococcus faecalis, pseudomonas fluorescens and salmonella enteritidis has been studied. in addition, the data on variability in microbial inactivation and duration of lag phase for surviving microbes have been fitted by normal and gamma distributions, respectively. the standard deviations of survivor number and lag phase duration of individual cells we ...201121798613
multidrug-resistant, extensively drug-resistant and pandrug-resistant bacteria: an international expert proposal for interim standard definitions for acquired resistance.clin microbiol infect abstract: many different definitions for multidrug-resistant (mdr), extensively drug-resistant (xdr) and pandrug-resistant (pdr) bacteria are being used in the medical literature to characterize the different patterns of resistance found in healthcare-associated, antimicrobial-resistant bacteria. a group of international experts came together through a joint initiative by the european centre for disease prevention and control (ecdc) and the centers for disease control and p ...201121793988
presence of glycopeptide-encoding plasmids in enterococcal isolates from food and humans in denmark.abstract enterococci and especially glycopeptides-resistant enterococci (gre) are a growing concern due to their ability to cause infections in hospitals. transmission of antimicrobial resistance between reservoirs such as animals, meat, and humans are in most cases linked to transmission of mobile genetic elements (mge) such as plasmids and transposons. presence of mge was tested in all gre isolated from food in denmark in 2005-2007 including the first vana mediated enterococcus faecalis isolat ...201121793656
experimental reproduction of enterococcal spondylitis in male broiler breeder chickens.there has been a recent emergence of epidemic spinal infections with necrosis causing lameness and mortality in male broilers and broiler breeders. mortality in affected flocks may be as high as 15%. the disease has been called enterococcal spondylitis (es), based on the frequent isolation of enterococcus cecorum from the lesions and necrosis and inflammation observed in the free thoracic vertebrae (ftv) of affected birds. male broiler breeders in an experimental setting were challenged with pur ...201121793445
unlocking the gates to inflammatory bowel disease: the role of enterococcus faecalis gelatinase. 201121791208
effectiveness of photodynamic therapy against enterococcus faecalis, with and without the use of an intracanal optical fiber: an in vitro study.abstract background and objective: photodynamic therapy (pdt) is a new technique introduced in endodontics that combines the action of a photosensitizer (dye) and a low intensity light source. currently, there are no pdt studies evaluating the microbial disinfection of root canals in order to compare the effects of light delivery systems in the photosensitizer activation. the aim of this study was to evaluate the pdt effectiveness in reducing enterococcus faecalis, with and without the aid of an ...201121790483
high-density fecal enterococcus faecium colonization in hospitalized patients is associated with the presence of the polyclonal subcluster cc17.enterococcus faecium belonging to the polyclonal subcluster cc17, with a typical ampicillin-resistant e. faecium (arefm) phenotype, have become prevalent among nosocomial infections around the world. high-density intestinal arefm colonization could be one of the factors contributing to the successful spread of these pathogens. we aimed to quantify the enterococcal intestinal colonization densities in stool samples from arefm-colonized and non-colonized patients using fluorescent in situ hybridiz ...201121789603
characterizing vancomycin resistant enterococcus (vre) strains with varying daptomycin (dap) resistance developed in an in vitro pharmacokinetic/pharmacodynamic model.two daptomycin (dap) regimens were evaluated in a pharmacokinetic/pharmacodynamic (pk/pd) model and recovered mutants were examined for changes in phenotypic characteristics. three enterococcus faecium strains (vre atcc 51559, vre 12311, and vre sf 12047) were utilized in a 7 day 1-compartment in vitro pk/pd model. simulated regimens were dap 6 mg/kg/day (fcmax=7.9 µg/ml, t1/2=8 h), and dap 10 mg/kg/day (fcmax=15.2 µg/ml, t1/2=8 h). samples were plated daily on mueller hinton agar containing dap ...201121788457
two novel species, enterococcus ureasum sp. nov. and enterococcus quebecensis sp. nov., isolated from water.three enterococcal isolates ccri-16620, ccri-16986(t) and ccri-16985(t) originating from water were characterized using morphological, biochemical and molecular taxonomic methods. 16s rrna gene sequence analysis classified all three strains in the enterococcus faecalis species group. the tree of 16s rrna sequences showed that the three isolates form two distinct clusters. the first cluster is constituted by strains ccri-16620 and ccri-16986(t) and the second cluster by the strain ccri-16985(t). ...201121788227
angiotensin-converting enzyme inhibitory activity in mexican fresco cheese.the objective of this study was to evaluate if mexican fresco cheese manufactured with specific lactic acid bacteria (lab) presented angiotensin i-converting enzyme inhibitory (acei) activity. water-soluble extracts (3 kda) obtained from mexican fresco cheese prepared with specific lab (lactococcus, lactobacillus, enterococcus, and mixtures: lactococcus-lactobacillus and lactococcus-enterococcus) were evaluated for acei activity. specific peptide fractions with high acei were analyzed using reve ...201121787916
evaluation of photodynamic therapy using a light-emitting diode lamp against enterococcus faecalis in extracted human teeth.photodynamic therapy (pdt) with high-power lasers as the light source has been proven to be effective in disinfecting root canals. the aim of this study was to evaluate the antimicrobial effect of pdt using toluidine blue o (tbo) and a low-energy light-emitting diode (led) lamp after the conventional disinfection protocol of 6% naocl.201121787505
application of an integrated community analysis approach for microbial source tracking in a coastal creek.high fecal indicator bacterial (fib) concentrations signal urban coastal water quality impairments that can threaten public health. however fib (total and fecal coliform plus enterococcus sp.) are not specific to human waste and thus microbial source tracking (mst) is employed to assess public health risks and remediation alternatives. currently, water quality diagnosis requires several simultaneous mst assays. relatively unexplored is a community analysis approach for mst where the overall micr ...201121786742
further increases in carbapenem-, amikacin-, and fluoroquinolone-resistant isolates of acinetobacter spp. and p. aeruginosa in korea: konsar study 2009.purpose: the increasing prevalence of antimicrobial resistant bacteria has become a serious worldwide problem. the aim of this study was to analyze antimicrobial resistance data generated in 2009 by hospitals and commercial laboratories participating in the korean nationwide surveillance of antimicrobial resistance program. materials and methods: susceptibility data were collected from 24 hospitals and two commercial laboratories. in the analysis, resistance did not include intermediate suscepti ...201121786445
pattern of bacterial and fungal infections in the first 3 months after pediatric living donor liver transplantation: an 11-year single-center experience.infection after pediatric living donor liver transplantation (ldlt) is a major cause of morbidity and mortality. here, we sought to determine the incidence, timing, location, and risk factors for bacterial and fungal infections. we retrospectively investigated infection for 3 postoperative months in 345 consecutive pediatric patients (56.2% were females) who underwent primary ldlt at kyoto university hospital, japan. a total of 179 patients (51.9%) developed at least 1 bacterial and/or fungal in ...201121786404
identification of a persistent, ta-independent tetracycline resistance-encoding plasmid from a dairy entercococcus faecium isolate.a tetracycline resistant (tet(r)) dairy enterococcus faecium isolate designated m7m2 was found to carry both tet(m) and tet(l) genes on a 19.6 kb plasmid. after consecutive transfer in the absence of tetracycline, the resistance-encoding plasmid persisted in 99% of the progenies. dna sequence analysis revealed that the 19.6 kb plasmid contained 28 open reading frames (orfs), including a tet(m)-tet(l)-mob gene cluster, as well as a 10.6 kb backbone highly homologous (99.9%) to the reported plasmi ...201121784909
infection or colonization and antimicrobial susceptibility of enterococcus spp at a regional hospital in queretaro, mexico. 201121782284
how many bacteria live on the keyboard of your computer? 201121782282
[antimicrobial activity of bacteriocin s760 produced by enterococcus faecium strain lwp760].antimicrobial activity of bacteriocin s760 (enterocin) produced by enterococcusfaecium strain lwp760 was studied. bacteriocin s760 is a cationic, hydrophobic, and heat stable peptide with the molecular weight of 5.5 kda and pl of 9.8. enterocin s760 is shown to inhibit in vitro the growth both of sensitive and resistant to antibacterials gramnegative and grampositive bacteria of 25 species. mics of the bacteriocin s760 vary between 0.05-1.6 mg/l for escherichia coli 0157:h117, salmonella typhimu ...201121780664
wheat- and barley-based diets with or without additives influence broiler chicken performance, nutrient digestibility and intestinal microflora.background: to our knowledge, there is scant literature on comparative broiler response to cereal diets high in soluble non-starch polysaccharides without or with enzyme, prebiotic, probiotic or synbiotic supplementation. in the present study, the effects of a wheat- and barley-based diet with or without supplemental xylanase plus ß-glucanase, inulin, enterococcus faecium or inulin plus enterococcus faecium, on bird performance, digesta viscosity, nutrient digestibility and intestinal microflora ...201121780133
probiotic enterococcus lactis iitrhr1 protects against acetaminophen-induced hepatotoxicity.objective: acetaminophen (apap), an antipyretic/analgesic drug, is reported to cause toxicity on overdose. dietary supplements are currently being explored to decrease toxicity. in the present study, the protective effect of probiotic enterococcus lactis iitrhr1 was evaluated at different doses (10(7), 10(8), and 10(9) colony-forming units) against apap-induced liver damage. methods: male wistar rats were administered apap (1 g/kg of body weight orally) for 14 d, and hepatotoxicity was assessed ...201121778036
peritonitis in children receiving continuous ambulatory peritoneal dialysis in northeast thailand.to clarify the peritonitis rate and to characterize the differences between the peritonitis and non-peritonitis group in esrd children using continuous ambulatory peritoneal dialysis (capd) in khon kaen, thailand.201121774284
in vitro antibacterial effect of aqueous and ethanolic moringa leaf extracts.to evaluate the antibacterial effect of aqueous and ethanolic moringa leaf extracts (moringa oleifera) on the growth of gram-positive and negative bacteria.201121771453
maldi-tof mass spectrometry-multivariate data analysis as a tool for classification of reactivation and non-culturable states of bacteria.some bacterial life states are only difficult to describe and to detect because they are on the border of active metabolism. a prominent example is the so-called viable but non-culturable state, which is mainly characterized by the inability of bacteria to grow on synthetic media. matrix-assisted laser desorption ionization time-of-flight mass spectrometry (maldi-tof/ms) in combination with multivariate data analysis represents a powerful tool for mass-spectrometric pattern recognition of biolog ...201121769553
potential role of fosfomycin in the treatment of community-acquired lower urinary tract infections caused by extended-spectrum ß-lactamase-producing escherichia coli.extended-spectrum ß-lactamase-producing escherichia coli (esblec) are emerging pathogens causing urinary tract infections (utis) in community patients worldwide. treatment for community-acquired esblec utis, especially in the outpatient setting, may be problematic because many of the strains are resistant to the traditional oral therapies. fosfomycin is an oral agent that is approved for the treatment of uncomplicated utis caused by enterococcus faecalis and e. coli. data evaluating the clinical ...201121768870
functional analysis of environmental dna-derived type ii polyketide synthases reveals structurally diverse secondary metabolites.a single gram of soil is predicted to contain thousands of unique bacterial species. the majority of these species remain recalcitrant to standard culture methods, prohibiting their use as sources of unique bioactive small molecules. the cloning and analysis of dna extracted directly from environmental samples (environmental dna, edna) provides a means of exploring the biosynthetic capacity of natural bacterial populations. environmental dna libraries contain large reservoirs of bacterial geneti ...201121768346
leukocytic responses and intestinal mucin dynamics of broilers protected with enterococcus faecium ef55 and challenged with salmonella enteritidis.the protective effect of enterococcus faecium ef55 in chickens challenged with salmonella enterica serovar enteritidis phage type 4 (se pt4) was assessed. the antibacterial effect on the bacterial microflora in the small intestine in relation to white blood cell count, phenotyping of peripheral blood and intestinal lymphocytes, functional activity of lymphocytes and phagocytes and mucin quantitation were investigated. day-old chicks (85) were randomly divided into four groups. the probiotic grou ...201121767856
enterococcus cecorum aortic valve endocarditis.we report a case of human aortic valve infective endocarditis caused by enterococcus cecorum. the organism was recovered from blood and identified by 16s ribosomal gene polymerase chain reaction (pcr). to the best of our knowledge, this is the first documented case of e. cecorum endocarditis. we wish to highlight the use of pcr for organism identification and to describe the initial misidentification of the pathogen.201121767709
comparison of direct cultivation on a selective solid medium, polymerase chain reaction from an enrichment broth, and the bd geneohm™ vanr assay for identification of vancomycin-resistant enterococci in screening specimens.fast and reliable diagnostics of vancomycin-resistant enterococci (vre) is an important prerequisite for containing vre transmission rates and controlling vre outbreaks among hospital patients. the bd geneohm™ vanr assay (becton dickinson diagnostics, erembodegem, belgium) is a real-time polymerase chain reaction (pcr) assay for screening perianal/rectal samples for the presence of vana or vanb genes that can be associated with vre. a set of 51 reference strains (vana-g genotypes) were correctly ...201121767707
tracking microbial transport through four onsite wastewater treatment systems to receiving waters in eastern north carolina.aims:  to examine microbial transport through properly functioning and failing onsite wastewater treatment systems (owts) and its implication in surrounding water quality. methods and results:  water samples were collected from monitoring wells near leach lines of owts and nearby ditches and receiving surface waters to analyse for escherichia coli and enterococci. tracer studies with rhodamine wt (rwt) and coliphage ms2 were also carried out to understand the fate and transport of contaminants t ...201121767338
net energy production associated with pathogen inactivation during mesophilic and thermophilic anaerobic digestion of sewage sludge.the potential for anaerobic digester energy production must be balanced with the sustainability of reusing the resultant biosolids for land application. mesophilic, thermophilic, temperature-phased, and high temperature (60 or 70 °c) batch pre-treatment digester configurations have been systematically evaluated for net energy production and pathogen inactivation potential. energy input requirements and net energy production were modeled for each digester scheme. first-order inactivation rate coe ...201121764416
selective immobilization of acinetobacter junii on the natural zeolitized tuff in municipal wastewater.the immobilization of desired bacteria onto material was usually performed in synthetic media. the aim of this study was to test the immobilization of phosphate (p)-accumulating bacteria acinetobacter junii onto natural zeolitized tuff (nz) in the raw or sterilized municipal wastewater containing the common bacteria escherichia coli and enterococcus faecalis and the performance of immobilized a. junii in the same type of wastewater. in the sterilized wastewater which contained the mixture of a. ...201121764269
rectal culture screening for vancomycin-resistant enterococcus in chronic haemodialysis patients: false-negative rates and duration of colonisation.infection or colonisation with vancomycin-resistant enterococci (vre) is common in chronic haemodialysis (hd) patients. however, there is limited information on the duration of vre colonisation or on the reliability of consecutive negative rectal cultures to determine the clearance of vre in chronic hd patients. chronic hd patients from whom vre was isolated were examined retrospectively. rectal cultures were collected more than three times, at least one week apart, between 1 june 2003 and 1 mar ...201121764175
effect of ultrasonics on enterococcus faecalis biofilm in a bovine tooth model.the purpose of this study was to evaluate in vitro the effect of the ultrasonic irrigation of sodium hypochlorite and edta in root canals of bovine teeth infected with enterococcus faecalis.201121763907
residual effectiveness of final irrigation regimens on enteroccus faecalis-infected root canals.the application of a final irrigating solution that remains active over a fairly long period of time stands as one strategy for preventing bacterial recolonization or eliminating the bacteria that persist after root canal treatment. the aim of this study was to evaluate the residual antimicrobial activity of four final irrigation regimens in root canals contaminated with enterococcus faecalis.201121763905
in vitro evaluation of the antibacterial activities of mtad in combination with nisin against enterococcus faecalis.mtad is a common intracanal irrigant. although mtad inhibits enterococcus faecalis in root canals, its bactericidal effect against e. faecalis remains to be improved. nisin, an antibacterial peptide, possesses a strong bactericidal effect. the study evaluated the synergetic action between mtad and nisin against e. faecalis.201121763904
novel probiotic enterococcus faecium is-27526 supplementation increased total salivary siga level and bodyweight of pre-school children: a pilot study.enterococcus faecium is-27526 is a novel probiotic isolated from dadih, an indonesian traditional fermented buffalo milk. a 90 days randomized double-blind placebo-controlled study ofpre-post trial was conducted in pre-school children with two groups, placebo and probiotic group. ultra high temperature low fat milk was used as a carrier in each group. the aims of this study were to investigate the effect of e.faecium is-27526 in milk on humoral immune response and on bodyweight of pre-school chi ...201121763445
characterization of the deoxynucleotide triphosphate triphosphohydrolase (dntpase) activity of the ef1143 protein from enterococcus faecalis and crystal structure of the activator-substrate complex.the ef1143 protein from enterococcus faecalis is a distant homolog of deoxynucleotide triphosphate triphosphohydrolases (dntpases) from escherichia coli and thermus thermophilus. these dntpases are important components in the regulation of the dntp pool in bacteria. biochemical assays of the ef1143 dntpase activity demonstrated nonspecific hydrolysis of all canonical dntps in the presence of mn2+. in contrast, with mg2+ hydrolysis required the presence of dgtp as an effector, activating the degr ...201121757692
evaluation of forty new phenothiazine derivatives for activity against intrinsic efflux pump systems of reference escherichia coli, salmonella enteritidis, enterococcus faecalis and staphylococcus aureus strains.because phenothiazines inhibit efflux pumps of bacteria, forty new phenothiazine derivatives were tested for their inhibition of the efflux pump systems of gram-positive and gram-negative pathogenic bacteria.201121753124
antioxidant, electrochemical, thermal, antimicrobial and alkane oxidation properties of tridentate schiff base ligands and their metal complexes.in this study, two schiff base ligands (hl(1) and hl(2)) and their cu(ii), co(ii), ni(ii), pd(ii) and ru(iii) metal complexes were synthesized and characterized by the analytical and spectroscopic methods. alkane oxidation activities of the metal complexes were studied on cyclohexane as substrate. the ligands and their metal complexes were evaluated for their antimicrobial activity against corynebacterium xerosis, bacillus brevis, bacillus megaterium, bacillus cereus, mycobacterium smegmatis, st ...201121752697
rupture of non-coronary sinus of valsalva with infective endocarditis of naive tricuspid valve presenting with recurrent ill-sustained ventricular tachycardia.infective endocarditis affecting the tricupid valve due to rupture of non-coronary sinus of valsalva is distinctly rare. we are reporting such a rare case, presenting with recurrent ill sustained ventricular tachycardia.201121751635
novel therapies for septic shock over the past 4 decades.infections that result in shock and organ failure are a major public health problem worldwide. severe sepsis and septic shock affect patients of all ages and often complicate chronic diseases. they are the major causes of death in critical care units and contribute substantially to hospital inpatient costs. translating the scientific advances of the last 4 decades into clinical practice has been challenging. despite many attempts to develop new therapies, the basic elements of treatment have not ...201121750297
bedbugs as vectors for drug-resistant bacteria.to the editor: over the past 10 years in north america and western europe, a resurgence of bedbugs (cimex lectularius) has occurred (1). although the basis for this resurgence is unclear, large bedbug infestations have been attributed to increased worldwide travel, altered insecticide management, and increased resistance to pesticides (2). marginalized populations in large urban centers appear to be disproportionately affected; ~30% of shelters in toronto, ontario, canada, report bedbugs (1). we ...201121749792
infection in immunocompromised hosts and the organ transplant recipient -essentials. 201121748845
microbiology at first visit of moderate-to-severe diabetic foot infection with antimicrobial activity and a survey of quinolone monotherapy.samples from 1295 patients with diabetic foot infection were evaluated; 4332 samples were collected with an average of 3.3 samples per patient. fifty-seven percent of patients had a 2b ulcer and 23% had a 3b ulcer according to texas university classification. in 64.2% of samples collected at first visit an etiologic agent was identified. about 40% of the positive samples were polymicrobial. gram positive bacteria were more frequently isolated (52.6%), staphylococcus aureus was the most frequentl ...201121824673
preparation of selenium/zinc-enriched probiotics and their effect on blood selenium and zinc concentrations, antioxidant capacities, and intestinal microflora in canine.the aim of this study was to prepare se/zn-enriched probiotics and investigate their effect on blood se and zn concentrations, blood antioxidant capacities, and intestinal microflora in canine. the preparation was performed in a single-factor experiment. the optimal culture conditions were as follows: the initial concentrations of se(4+) and zn(2+) were 5 and 150 µg/ml, respectively; the inoculation volume was 5%; and the liquid volume of the medium was 50 ml in a 250-ml flask. the medium was th ...201120563665
cadmium chloride exhibits a profound toxic effect on bacterial microflora of the mice gastrointestinal tract.cadmium (cd²(+)), a naturally occurring heavy metal, is an important environmental pollutant and a potent toxicant to bacteria. the gastrointestinal (gi) tract microflora has a marked capacity to cope with the increased load of ingested metals. however, heavy metals may have harmful effects on git microflora. under the conditions of experimental exposure to cadmium, changes in the population of intestinal microflora in healthy mice were examined. five experimental groups received 23 to 50 mg kg⁻ ...201120488844
a cross-sectional survey of snake oral bacterial flora from hong kong, sar, china.to determine the pattern of oral bacterial flora and their sensitivity to antibiotics in freshly captured native snakes in hong kong sar, people's republic of china.201120511640
prophylactic cefdinir for pediatric cases of complicated urinary tract infection.this study evaluated the effect of prophylactic cefdinir (3 mg/kg given once daily) for the prevention of recurrent and complicated urinary tract infections (uti) in pediatric patients.201120573040
formulation and bacterial phototoxicity of curcumin loaded alginate foams for wound treatment applications: studies on curcumin and curcuminoides xlii.curcumin loaded alginate foams are proposed for application in antimicrobial photodynamic therapy of infected wounds. the drug loaded foams were formulated to provide a burst release of the photosensitizer when hydrated. the foams remained intact after hydration and would be possible to remove from the wound prior to irradiation without causing any tissue damage. the characterization of the prepared foams showed that both curcumin loaded and unloaded foams hydrated within 1  min and absorbed fro ...201120575064
linum usitatissimum (linseed/flaxseed) fixed oil: antimicrobial activity and efficacy in bovine mastitis.in vitro antimicrobial activity and in vivo therapeutic efficacy of l. usitatissimum (linseed/flaxseed) fixed oil in bovine mastitis were investigated.201120602175
perioperative synbiotic treatment to prevent infectious complications in patients after elective living donor liver transplantation: a prospective randomized study.although the effect of synbiotic therapy using prebiotics and probiotics has been reported in hepatobiliary surgery, there are no reports of the effect on elective living-donor liver transplantation (ldlt).201120619394
antibacterial activity of extracts from plants of central argentina--isolation of an active principle from achyrocline satureioides.the great increase in bacterial infections is fueling interest in the search for antibacterial products of plant origin. extracts obtained from 51 native and naturalized plants from central argentina were therefore evaluated for their in vitro inhibitory activity on pathogenic bacteria with the aim of selecting the most active ones as new sources of effective antibiotics. the susceptibility of reference and clinical strains of enterococcus faecalis, escherichia coli, proteus mirabilis, pseudomon ...201120645245
evaluation of selenite bioremoval from liquid culture by enterococcus species.the genus enterococcus belong to the genera of bacteria that produce lactic acid and can confer health benefits to living organisms. selenium (se) is an essential micronutrient for humans and animals. thirty-six enterococcus species isolated from dairy products were screened for se(iv) sorption capacity for use as a probiotics in animal nutrition. several isolates grew luxuriantly and significantly removed se(iv) from se(iv) amended medium. two isolates, lab 14 and lab 18, identified by 16s rrna ...201120634050
in vitro antioxidant and antibacterial properties of hydrolysed proteins of delimed tannery fleshings: comparison of acid hydrolysis and fermentation methods.proteins in delimed tannery fleshings were fermentatively hydrolysed using enterococcus faecium ncim5335 and also hydrolysed using mild organic acids (formic acid and propionic acid). the liquor portion containing hydrolysed proteins was spray dried, in both the cases, to obtain a powder. the spray dried powder was evaluated for in vitro antioxidant activities with respect to scavenging different free radicals and antibacterial properties against nine different pathogens. fermentation and acid h ...201120680665
risk factors for fluoroquinolone resistance in enterococcus urinary tract infections in hospitalized patients.summarypast studies exploring risk factors for fluoroquinolone (fq) resistance in urinary tract infections (utis) focused only on utis caused by gram-negative pathogens. the epidemiology of fq resistance in enterococcal utis has not been studied. we conducted a case-control study at two medical centres within the university of pennsylvania health system in order to identify risk factors for fq resistance in enterococcal utis. subjects with positive urine cultures for enterococci and meeting cdc ...201120696087
oral administration of clostridium butyricum for modulating gastrointestinal microflora in mice.this study aimed to evaluate the safety of clostridium butyricum and to investigate the effect of c. butyricum on mice ecosystem in the intestinal tract by way of examining the population of different microorganisms isolated from caecal contents. we firstly evaluated the safety of c. butyricum using acute toxicity test and ames test. then forty male balb/c mice were divided into the following four treatment groups, each consisting of ten mice: normal group, low-dose group, medium-dose group and ...201120711781
clinical characteristics and outcomes of patients with vancomycin-susceptible enterococcus faecalis and enterococcus faecium bacteraemia in cancer patients.the purpose of this investigation was to compare the risk factors, clinical features and outcomes in cancer patients with bacteraemia caused by vancomycin-susceptible enterococcus faecalis and e. faecium. a retrospective, observational 7-year study was carried out in a 450-bed, acute-care university-affiliated hospital. we performed univariate comparisons between the two groups and then multivariate analysis to identify patient risk factors for e. faecium isolation. seventy-three patients were i ...201120711795
highlighting new phylogenetic specificities of crohn's disease microbiota.recent studies suggest that gastrointestinal (gi) microbes play a part in the pathogenesis of crohn's disease (cd).201120722058
microbiological findings of culture-positive preservation fluid in liver transplantation.bacterial and fungal infections are the leading cause of mortality in liver transplant (lt) recipients. few studies have examined the incidence of culture-positive preservation fluid (pf) and the outcome of related recipients. the aim of this study was to determine the incidence and the microbiologic findings of pf positive cultures, and to evaluate the impact on morbidity and mortality of lt recipients. a retrospective analysis of pf cultures performed after 477 lts from cadaveric grafts betwee ...201120738832
isolation and purification of two bacteriocins 3d produced by enterococcus faecium with inhibitory activity against listeria monocytogenes.strain 3d, isolated from fermented traditional moroccan dairy product, and identified as enterococcus faecium, was studied for its capability to produce two bacteriocins acting against listeria monocytogenes. bacteriocins 3 da and 3db were heat stable inactivated by proteinase k, pepsin, and trypsin but not when treated with catalase. the evidenced bacteriocins were stable in a wide ph range from 2 to 11 and bactericidal activity was kept during storage at 4°c. however, the combination of temper ...201120717676
pathogen removal in farm-scale psychrophilic anaerobic digesters processing swine manure.this study assessed the efficiency of commercial-scale psychrophilic anaerobic digestion in sequencing batch reactors (padsbrs) for pathogen removal from pig manure. the impact of treatment cycle length and of hydraulic flow regimes on pathogen removal efficiency was investigated. two conventionally operated sbrs (br1 and br2) and two sbrs simultaneously fed during the draw step (br3 and br4) were monitored over a two-year period. padsbrs significantly decreased the concentration of coliforms, s ...201120801022
bactericidal effect of a nd:yag laser on enterococcus faecalis at pulse durations of 15 and 25 ms in dentine depths of 500 and 1,000 μm.the success of endodontic treatment depends on the effective elimination of microorganisms from the root canal, and lasers provide more effective disinfection than conventional treatment using rinsing solutions. the objective of this in vitro study was to determine the bactericidal effect of laser irradiation in dentine of various depths at a wavelength of 1,064 nm and pulse durations of 15 and 25 ms. a total of 90 dentine slices were cut from bovine incisors and divided into two groups (45 slic ...201120809081
effect of iptg amount on apo- and holo- forms of glycerophosphate oxidase expressed in escherichia coli.escherichia coli has proved to be a successful host for the expression of many heterologous proteins, and much efforts have been made toward improving recombinant protein expression including the usage of strong promoters and co-expression with chaperones. but little attention was paid on the relation between expression level and function of the target protein. glycerophosphate oxidase (gpo) is a protein with fad cofactor (without free cysteine and disulfide bonds).it was observed that the speci ...201120736068
a laboratory evaluation of the antibacterial and cytotoxic effect of liquorice when used as root canal medicament.to evaluate the antibacterial and cytotoxic effects of liquorice as a root canal medicament and to compare its action to the commonly used root canal medicament calcium hydroxide ca(oh)(2).201120812941
molecular analysis of linezolid resistance in clinical enterococcus faecium isolates by polymerase chain reaction and pyrosequencing.resistance to linezolid has been associated with a g2576t mutation in the 23 s rrna gene. clinical isolates of linezolid-sensitive and linezolid-resistant vancomycin-resistant enterococcus faecium of a liver transplant patient have been analysed for the g2576t mutation by polymerase chain reaction restriction fragment length polymorphism (pcr-rflp), conventional sequencing and pyrosequencing. a clear association between the number of mutated 23 s rrna genes and the level of linezolid resistance ...201120814709
opportunistic bacterial infections in breeding colonies of the nsg mouse strain.spontaneous morbidity primarily affecting female breeders in 3 independent breeding colonies of nsg (nod.cg-prkdc(scid) i12rg(tm1wjl) /szj) mice prompted an investigation to uncover the cause of disease. necropsies were performed on 264 (157 female and 107 male) spontaneously sick, experimentally unmanipulated nsg mice. in sum, 42 mice (15.9%) had acute or chronic renal inflammatory lesions, of which 12 had concurrent histologic evidence of an ascending urinary tract infection. from 94 kidneys c ...201120817888
[implementation of vana and vanb genes by pcr technique research interest in system (xpert vana/vanb cepheidr) closed in a laboratory of microbiology in managing an outbreak to enterococcus faecium resistant glycopeptide (efrg)].the closed system pcr for the rapid detection of vana and vanb genes (xpert vana/vanb cepheid(®)) was evaluated in our laboratory, to improve the rapidity of the response and thus the management of patients and isolation measures during two gre outbreaks.201120828941
nitrite pickling salt as an alternative to formaldehyde for embalming in veterinary anatomy--a study based on histo- and microbiological analyses.formaldehyde, the traditional embalming agent has negative health effects. nitrite pickling salt has been reported to be a good and inexpensive alternative when supplemented with antioxidants, but the antioxidants caused yellowish colouration of cadavers, and damaged corrosion-resistant steel tables and stone floors. here, nitrite pickling salt was supplemented with ethanol and pluriol(®) and tested for effectiveness as an embalming agent of twenty dog cadavers: 10 with open, and 10 with closed ...201120829010
bactericidal effects of non-thermal argon plasma in vitro, in biofilms and in the animal model of infected wounds.non-thermal (low-temperature) physical plasma is under intensive study as an alternative approach to control superficial wound and skin infections when the effectiveness of chemical agents is weak due to natural pathogen or biofilm resistance. the purpose of this study was to test the individual susceptibility of pathogenic bacteria to non-thermal argon plasma and to measure the effectiveness of plasma treatments against bacteria in biofilms and on wound surfaces. overall, gram-negative bacteria ...201120829396
risk factors for hospital-acquired urinary tract infection: a case-control study.the objective is to assess risk factors and microbiological aspects of hospital-acquired urinary tract infection (hauti) on six wards of a general regional hospital in serbia. a case-control study was nested within prospective cohort hautis study conducted from january 1 to december 31, 2007. three controls were identified for each patient with hauti, being chronologically the next three patients surveyed who did not develop hauti. the patients and controls were matched by sex and age (±5 years) ...201120848199
addressing uncertainty in fecal indicator bacteria dark inactivation rates.assessing the potential threat of fecal contamination in surface water often depends on model forecasts which assume that fecal indicator bacteria (fib, a proxy for the concentration of pathogens found in fecal contamination from warm-blooded animals) are lost or removed from the water column at a certain rate (often referred to as an "inactivation" rate). in efforts to reduce human health risks in these water bodies, regulators enforce limits on easily-measured fib concentrations, commonly repo ...201120843534
[etiology and sensitivity of uropathogens identified in uncomplicated lower urinary tract infections in women (aresc study): implications on empiric therapy].to determine the etiology and susceptibility of uropathogens identified in women with uncomplicated lower urinary tract infections (uti).201120889171
usefulness of 99mtc-ciprofloxacin scintigraphy in the diagnosis of prosthetic joint infections.to evaluate the usefulness of 99tc-ciprofloxacin scintigraphy (cfs) in patients with hip or knee arthroplasty and suspected infection.201120975609
late-onset deep infectious keratitis after descemet stripping endothelial keratoplasty with vent incisions.to report the clinical and histopathological findings of 3 cases of late-onset deep infectious keratitis after descemet stripping endothelial keratoplasty (dsek) with vent incisions.201121045667
isolation and characterisation of lactic acid bacterium for effective fermentation of cellobiose into optically pure homo l-(+)-lactic acid.effective utilisation of cellulosic biomasses for economical lactic acid production requires a microorganism with potential ability to utilise efficiently its major components, glucose and cellobiose. amongst 631 strains isolated from different environmental samples, strain qu 25 produced high yields of l-(+)-lactic acid of high optical purity from cellobiose. the qu 25 strain was identified as enterococcus mundtii based on its sugar fermentation pattern and 16s rdna sequence. the production of ...201121061005
antimicrobial characterisation of solithromycin (cem-101), a novel fluoroketolide: activity against staphylococci and enterococci.solithromycin (cem-101) is a novel fluoroketolide with high potency against gram-positive and gram-negative bacteria commonly associated with community-acquired respiratory tract infections and skin and skin-structure infections. in this study, solithromycin and comparator antimicrobials were tested against a contemporary collection of staphylococcus aureus, coagulase-negative staphylococci, enterococcus faecalis, enterococcus faecium and other enterococcus spp. collected in the sentry antimicro ...201121075602
incidence and risk factors of vancomycin-resistant enterococcus colonization in burn unit patients.this study was aimed to identify the incidence of vancomycin-resistant enterococcus (vre) colonization in burn patients, to collate risk factors for colonization and to determine the vre resistance profile to different antimicrobial agents. this prospective study was carried out on the burn unit, during the period from september 2008 to january 2010, in 128 patients who were hospitalized at least 3 weeks or more. periodic swabs were taken from burn wound, rectal, axillary, umblicaly and throat r ...201120926196
loss of vancomycin resistance not completely dependent on the tn1546 element in enterococcus faecium isolates.we investigated characteristics of 3 enterococcus faecium strains (shy-1, shy-2, and shy-3) isolated successively from 1 patient. in vitro susceptibility testing was performed using broth microdilution method. change of vancomycin mic was monitored during incubation with vancomycin for shy-3 strain. genetic backgrounds were determined both by multilocus sequence typing (mlst) and pulsed-field gel electrophoresis (pfge). in addition, the genetic variations among tn1546 element were investigated b ...201121146722
infective endocarditis in chronic hemodialysis patients: experience from morocco.since the 1960s, regular hemodialysis (hd) was recognized as a risk factor for the development of infective endocarditis (ie), particularly at vascular access sites. the present report describes our experience at the etat major general agadir, morocco, of taking care of ie in patients on regular dialysis. a retrospective analysis was made of five cases of ie in patients receiving regular hd having arteriovenous fistula as vascular access. they were sent from four private centers and admitted in ...201121196639
residual antibacterial activity of a new modified sodium hypochlorite-based endodontic irrigation solution.in this in vitro study the antibacterial substantivity of a new sodium hypochlorite-based root canal irrigant (hypoclean) in bovine root dentin was investigated.201121196865
the annotated complete dna sequence of enterococcus faecalis bacteriophage φef11 and its comparison with all available phage and predicted prophage genomes.φef11 is a temperate siphoviridae bacteriophage isolated by induction from a lysogenic enterococcus faecalis strain. the φef11 dna was completely sequenced and found to be 42,822 bp in length, with a g+c mol% of 34.4%. genome analysis revealed 65 orfs, accounting for 92.8% of the dna content. all except for seven of the orfs displayed sequence similarities to previously characterized proteins. the genes were arranged in functional modules, organized similar to that of several other phages of low ...201121204936
anti-listeria bacteriocin-producing bacteria from raw ewe's milk in northern greece.the isolation and partial characterization of anti-listeria bacteriocin producing strains present in milk from areas of northern greece in view of their potential use as protective cultures in food fermentations.201121205100
comparative genomic analysis reveals significant enrichment of mobile genetic elements and genes encoding surface structure-proteins in hospital-associated clonal complex 2 enterococcus faecalis.enterococci rank among the leading causes of nosocomial infections. the failure to identify pathogen-specific genes in enterococcus faecalis has led to a hypothesis where the virulence of different strains may be linked to strain-specific genes, and where the combined endeavor of the different gene-sets result in the ability to cause infection. population structure studies by multilocus sequence typing have defined distinct clonal complexes (cc) of e. faecalis enriched in hospitalized patients ( ...201121205308
quantifying environmental reservoirs of fecal indicator bacteria associated with sediment and submerged aquatic vegetation.elevated concentrations of fecal indicator bacteria (fib) in aquatic sediments and vegetation have prompted concern that environmental reservoirs of fib disrupt the correlation between indicator organisms, pathogens and human health risks. fib numbers, however, are typically normalized to volume of water or mass of substrate. because these reservoirs tend to differ greatly in magnitude within and between water bodies, direct comparison between water column and benthic population sizes can be pro ...201121208357
risk factors for infections requiring hospitalization in renal transplant recipients: a cohort study.our aim was to analyze a retrospective cohort of renal transplant recipients to determine the risk factors for infections that require hospitalization.201121208820
endogenous endophthalmitis caused by enterococcus casseliflavus.herein, we report a case of endogenous endophthalmitis caused by enterococcus casseliflavus. the organism was sensitive to gentamicin, vancomycin and chloramphenicol and resistant to cefazolin, ofloxacin, gatifloxacin and ciprofloxacin. the patient was successfully treated with vitrectomy and sensitivity-based intravitreal vancomycin.201121212141
characterization and transfer of antibiotic resistance in lactic acid bacteria from fermented food products.the study provides phenotypic and molecular analyses of the antibiotic resistance in lactic acid bacteria (lab) from fermented foods in xi'an, china. lab strains (n = 84) belonging to 16 species of lactobacillus (n = 73), and streptococcus thermophilus (n = 11) were isolated and identified by sequencing their 16s rrna gene. all strains were susceptible to ampicillin, bacitracin, and cefsulodin, and intrinsically resistant to nalidixic acid, kanamycin, and vancomycin (except l. bulgaricus, l. aci ...201121212956
virulence factors in enterococci from partridges (alectoris rufa) representing a food safety problem.forty-three enterococcus isolates recovered from fecal samples of partridges, during the partridge hunting season, were studied for gelatinase and β-hemolysis activities. the presence of fsr-gele genes and the cyl operon was studied by polymerase chain reaction and correlated with gelatinase and β-hemolysis production, respectively. in addition, genes encoding additional virulence factors (cpd, hyl, agg, esp, and ace) was also analyzed in all enterococci. most of our enterococcus faecalis isolat ...201121214387
a surgical case of excision of infected aneurysm arising from anterior interosseal artery following infectious endocarditis.infected aneurysm (ia) of the anterior interosseal artery (aia), the first branch of the ulnar artery, is an infrequent but serious complication of infectious endocarditis (ie). we report a successful case of excision of ia arising from aia. in this case, the ia expanded and adhered to the ulnar artery, resulting in occlusion of the ulnar artery. reconstruction of the ulnar artery was not needed by the preoperative evaluation and the intraoperative occlusion testing. we discuss surgical treatmen ...201121215573
design and optimization of fermentation medium for enhanced bacteriocin production by probiotic bacterium enterococcus faecium mc13.statistics-based experimental designs were used to develop a cost-effective medium for enhanced production of viable cells and bacteriocin by probiotic enterococcus faecium mc13. carbon, nitrogen, and mineral sources were first screened by one-variable-at-a-time (ovat) methods. in order to increase yield production, the selected variables were further statistically optimized using response-surface methodology (rsm) with central composite design (ccd). the maximum and minimum levels of the select ...201121229463
antimicrobial activity of diterpenes from viguiera arenaria against endodontic bacteria.six pimarane-type diterpenes isolated from viguiera arenaria baker and two semi-synthetic derivatives were evaluated in vitro against a panel of representative microorganisms responsible for dental root canal infections. the microdilution method was used for the determination of the minimum inhibitory concentration (mic) and minimum bactericidal concentration (mbc) against porphyromonas gingivalis, prevotella nigrescens, prevotella intermedia, prevotella buccae, fusobacterium nucleatum, bacteroi ...201121233793
vancomycin-resistance transferability from vana enterococci to staphylococcus aureus.in last decade methicillin-resistant staphylococcus aureus with high level of vancomycin-resistance (vrsa) have been reported and generally the patients with vrsa infection were also infected with a vancomycin-resistant enterococcus (vre). considering that the high level of vancomycin-resistance in vrsa isolates seems to involve the horizontal transfer of tn1546 transposon containing vana gene from coinfecting vre strains, the authors have studied the "in vitro" conjugative transfer of this resi ...201121234755
carijodienone from the octocoral carijoa multiflora. a spiropregnane-based steroid.two new steroids (1 and 2) and the known pregna-1,4,20-trien-3-one (3) have been isolated from the pacific octocoral carijoa multiflora. compound 1 possesses a novel spiropregnane-based steroidal skeleton. the photochemical transformation of 3 into 1 allowed the assignment of the absolute configuration at c-10 of 1. the antibacterial activities of compounds 1 and 3 were evaluated against a panel of bacterial strains.201121235217
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