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early emergency care. 20103105703
continuous subcutaneous insulin infusion versus injection therapy: a randomized cross-over trial under usual diabetic clinic conditions.twelve c-peptide negative insulin-dependent diabetic patients participated in a randomized cross-over study of 6 months treatment with twice or thrice daily insulin injection therapy and continuous subcutaneous insulin infusion (csii). standard, non-intensified management conditions were maintained throughout. glycosylated haemoglobin levels were similar on both regimens (9.2 +/- 0.5% versus 9.0 +/- 0.4%; csii vs injection therapy; (mean +/- sem). capillary blood glucose concentrations before br ...20102962807
wellcome developments in tropical medicine. 20103105672
wellcome developments in tropical medicine. 20103105672
[laboratory diagnosis of lysosomal storage disease (glycoproteinosis pompe's disease and gaucher disease)]. 20102940012
benign familial haematuria. 20102900928
[prevention of stress ulcer and nosocomial pneumonia]. 20102905096
treatment of silent myocardial ischemia with transdermal nitroglycerin added to beta-blockers and alprazolam.investigations into the mechanisms and characteristics of ischemic heart disease have increasingly documented evidence of myocardial ischemia in the absence of symptoms. recent work using objective criteria of ischemic episodes confirmed that angina pectoris or its equivalents need not accompany myocardial ischemia and noted that these episodes appear to be quite common. the impact on prognosis awaits further study, but preliminary data suggest an adverse prognosis for patients with recurrent sp ...20102877735
hypophosphatemic rickets/osteomalacia in linear sebaceous nevus syndrome: a variant of tumor-induced osteomalacia.a severe form of vitamin d-resistant rickets is associated with the linear sebaceous nevus syndrome. we investigated the pathophysiology underlying defective bone mineralization in two individuals and examined the effects of 1,25-dihydroxyvitamin d (1,25(oh)2d, calcitriol) therapy on the clinical and biochemical abnormalities. both patients had fasting hypophosphatemia, markedly diminished tmp/gfr, and elevated alkaline phosphase activity in the presence of normocalcemia. before treatment with c ...20103023599
interactions of anticancer quinone drugs, aclacinomycin a, adriamycin, carbazilquinone, and mitomycin c, with nadph-cytochrome p-450 reductase, xanthine oxidase and oxygen.the properties of the interactions of anticancer quinone drugs, aclacinomycin a, adriamycin, carbazilquinone, and mitomycin c with nicotinamide adenine dinucleotide phosphate (nadph)-cytochrome p-450 reductase and xanthine oxidase under anaerobic and aerobic conditions were studied. km values of nadph-cytochrome p-450 reductase for these drugs were in the range of 40-227 microm, and that of deflavo xanthine oxidase in the range of 39-over 200 microm. under anaerobic conditions, when xanthine was ...20103023600
abnormalities of mitochondrial enzymes in hereditary ataxias.the activity of 7 mitochondrial enzymes, fumarase, nad-malate dehydrogenase (mdh), citrate synthase (cs), valine dehydrogenase (vdh), succinate dehydrogenase (sdh), glutamate dehydrogenase (gdh), pyruvate dehydrogenase complex (pdhc) has been measured in platelet preparations from patients affected by friedreich's ataxia (fa), dominant and non-dominant olivopontocerebellar atrophy (dopca, ndopca) and normal individuals. significant decreases of gdh (p less than 0.01), pdhc (p less than 0.01), vd ...20102816270
intestinal dysbiosis and reduced immunoglobulin-coated bacteria associated with coeliac disease in children.coeliac disease is a chronic intestinal inflammatory disorder due to an aberrant immune response to dietary gluten proteins in genetically predisposed individuals. mucosal immune response through iga secretion constitutes a first line of defence responsible for neutralizing noxious antigens and pathogens. the aim of this study was the characterization of the relationships between immunoglobulin-coated bacteria and bacterial composition of faeces of coeliac disease (cd) patients, untreated and tr ...201020181275
selected microbial groups and short-chain fatty acids profile in a simulated chicken cecum supplemented with two strains of lactobacillus.among the bacterial fermentation end products in the chicken cecum, butyrate is of particular importance because of its nutritional properties for the epithelial cell and pathogen inhibitory effects in the gut. an in vitro experiment, operated with batch bioreactor, was conducted to quantify butyric-producing bacteria in a simulated broiler cecum supplemented with lactobacillus salivarius ssp. salicinius jcm 1230 and lactobacillus agilis jcm 1048 during 24 h of incubation. selected bacterial spe ...201020181862
prebiotic effect of fruit and vegetable shots containing jerusalem artichoke inulin: a human intervention study.the present study aimed to determine the prebiotic effect of fruit and vegetable shots containing inulin derived from jerusalem artichoke (ja). a three-arm parallel, placebo-controlled, double-blind study was carried out with sixty-six healthy human volunteers (thirty-three men and thirty-three women, age range: 18-50 years). subjects were randomised into three groups (n 22) assigned to consume either the test shots, pear-carrot-sea buckthorn (pcs) or plum-pear-beetroot (ppb), containing ja inul ...201020187995
bacterial flora in inflammatory bowel disease.the pathogenesis of inflammatory bowel disease (ibd) involves an interaction between host susceptibility (which is partly genetically determined), mucosal immunity and the intestinal milieu. micro-organisms have physiological effects on mucosal structure, epithelial turnover, the intestinal immune cells and, thus, on many intestinal functions. toll-like receptors and nucleotide oligomerisation-binding domain proteins in host cells recognise specific bacterial molecules and modify the immune resp ...201020203504
association between faecalibacterium prausnitzii and dietary fibre in colonic fermentation in healthy human subjects.the intestinal microbiota are a complex ecosystem influencing the immunoregulation of the human host, providing protection from colonising pathogens and producing scfa as the main energy source of colonocytes. our objective was to investigate the effect of dietary fibre exclusion and supplementation on the intestinal microbiota and scfa concentrations. faecal samples were obtained from healthy volunteers before and after two 14 d periods of consuming formulated diets devoid or supplemented with ...201020346190
microbiota in pediatric inflammatory bowel disease.to test the hypothesis that compared with controls, children with inflammatory bowel disease (ibd) exhibit differences in the relationships between gut microbiota and disease activity.201020400104
quantification of faecalibacterium prausnitzii- and subdoligranulum variabile-like bacteria in the cecum of chickens by real-time pcr.the intestinal microbial community is playing an important role in health and production performance of chickens. to understand the effect on the intestinal microflora induced by various feeding strategies, feed additives, infections, and intestinal disorders, it is important to have methods for quantifying potentially important bacteria in the intestine. we describe a real-time quantitative assay for detection and quantification of a whole group of faecalibacterium prausnitzii and subdoligranul ...201020460669
the bacteriology of pouchitis: a molecular phylogenetic analysis using 16s rrna gene cloning and sequencing.to identify, compare, and contrast the microbiota in patients with and without pouchitis after restorative proctocolectomy (rpc) for ulcerative colitis (uc) and familial adenomatous polyposis (fap).201020562611
is the abundance of faecalibacterium prausnitzii relevant to crohn's disease?reports that bacteria within the firmicutes phylum, especially the species faecalibacterium prausnitzii, are less abundant in crohn's disease (cd) patients and supernatants from cultures of this bacterium are anti-inflammatory prompted the investigation of the possible correlations between the abundance of f. prausnitzii and the response to treatment in patients with gut diseases and healthy controls. in a randomized, double-blind trial, faeces were collected from healthy volunteers, and from pa ...201020695899
[surgery in the treatment of patients with small cell lung cancer].the data on surgical and combined treatment of 52 cases of small cell lung cancer are presented and the end results are evaluated. the findings on survival time make the case for surgery as a component of combined treatment of tumor. the best results were obtained in cases of stage i and ii tumor, absence of metastases into intrathoracic lymph nodes, tumor invasion limited to bronchopulmonary lymph nodes or the root of the lung, intermediate cell pattern of small cell lung cancer and surgery fol ...20102998088
[home-made simple automatic flow-time counter for viscosity measurement]. 20102956340
[drug damage of the liver]. 20102873488
limited tryptic digestion of acanthamoeba myosin ia abolishes regulation of actin-activated atpase activity by heavy chain phosphorylation.acanthamoeba myosin ia is a globular protein composed of a 140-kda heavy chain and a 17-kda light chain. it expresses high actin-activated mg2+-atpase activity when one serine on the heavy chain is phosphorylated. we previously showed that chymotrypsin cleaves the heavy chain into a cooh-terminal 27-kda peptide that can bind to f-actin but has no atpase activity and a complex containing the nh2-terminal 112-kda peptide and the light chain. the complex also binds f-actin and has full actin-activa ...20102958454
communicating leveled clinical expectations to nursing students.nurse educators agree on the need for objectives in the classroom situation and frequently communicate them in a written form. written expectations for clinical, however, are rarely given to student nurses prior to their clinical experience. clinical expectations are understood by instructors, but communicating them to students result in mixed interpretations. the article describes the development of a written method of communicating clinical expectations to solve this problem. this method corre ...20103035139
diets supplemented with chickpea or its main oligosaccharide component raffinose modify faecal microbial composition in healthy adults.the effects of diets supplemented with either chickpea or its main oligosaccharide raffinose on the composition of the faecal microbial community were examined in 12 healthy adults (18-65 years) in a randomised crossover intervention study. subjects consumed their usual diet supplemented with soups and desserts that were unfortified, or fortified with either 200 g/d of canned chickpeas or 5 g/d of raffinose for 3 week periods. changes in faecal bacterial populations of subjects were examined usi ...201021831757
dysbiosis of fecal microbiota in crohn's disease patients as revealed by a custom phylogenetic microarray.a custom phylogenetic microarray composed of small subunit ribosomal rna probes, representing ≈500 bacterial species from the human and animal gut, was developed and evaluated for analysis of gut microbial diversity using fecal samples from healthy subjects and crohn's disease (cd) patients.201020848492
rifaximin modulates the colonic microbiota of patients with crohn's disease: an in vitro approach using a continuous culture colonic model system.rifaximin, a rifamycin derivative, has been reported to induce clinical remission of active crohn's disease (cd), a chronic inflammatory bowel disorder. in order to understand how rifaximin affects the colonic microbiota and its metabolism, an in vitro human colonic model system was used in this study.201020852272
the role of mucosal immunity and host genetics in defining intestinal commensal bacteria.dramatic advances in molecular characterization of the largely noncultivable enteric microbiota have facilitated better understanding of the composition of this complex ecosystem at broad phylogenetic levels. this review outlines current understanding of mechanisms by which commensal bacteria are controlled and shaped into functional communities by innate and adaptive immune responses, antimicrobial peptides produced by epithelial cells and host genetic factors.201020871399
differential adaptation of human gut microbiota to bariatric surgery-induced weight loss: links with metabolic and low-grade inflammation markers.obesity alters gut microbiota ecology and associates with low-grade inflammation in humans. roux-en-y gastric bypass (rygb) surgery is one of the most efficient procedures for the treatment of morbid obesity resulting in drastic weight loss and improvement of metabolic and inflammatory status. we analyzed the impact of rygb on the modifications of gut microbiota and examined links with adaptations associated with this procedure.201020876719
[the human intestinal microbiota].the human intestinal microbiota constitutes a complex ecosystem which is now well recognized for its impact on human health and well-being. it contributes to maturation of the immune system and provides a direct barrier against colonization by pathogens. its possible implication in diseases of modern societies, currently increasing in prevalence, has been reported. these include allergies, inflammatory bowel diseases and possibly metabolic and degenerative disorders. the analysis of the molecula ...201020889008
[functional biostructure of colonic microbiota (central fermenting area, germinal stock area and separating mucus layer) in healthy subjects and patients with diarrhea treated with saccharomyces boulardii].the colonic content can be compared to a spatially structured high output bioreactor composed of three functionally different regions: a separating mucus layer, a germinal stock area, and a central fermenting area. the stool mirrors this structure and can be used for diagnosis in health and disease. in a first part, we introduce a novel method based on fluorescence in situ hybridization (fish) of sections of punched-out stool cylinders, which allows quantitatively monitor microbiota in the mucus ...201020889010
the role of bacteria in the pathogenesis of inflammatory bowel disease.crohn's disease (cd) and ulcerative colitis (uc) have features that suggest bacterial involvement, and all genetic models of inflammatory bowel disease (ibd) require the presence of commensal bacteria. cd is associated with innate immune response genes such as nod2/card15 and the autophagy genes atg16l1 and irgm. however, ibd responds to immunosuppression, suggesting that any bacteria involved are not acting as conventional pathogens. molecular techniques are rapidly advancing our knowledge of t ...201020981205
numerical ecology validates a biogeographical distribution and gender-based effect on mucosa-associated bacteria along the human colon.we applied constrained ordination numerical ecology methods to data produced with a human intestinal tract-specific phylogenetic microarray (the aus-hit chip) to examine the microbial diversity associated with matched biopsy tissue samples taken from the caecum, transverse colon, sigmoid colon and rectum of 10 healthy patients. consistent with previous studies, the profiles revealed a marked intersubject variability; however, the numerical ecology methods of analysis allowed the subtraction of t ...201021124491
[16s rrna gene-based molecular methods to monitor clostridium cluster iv community in the colon of piglets].to investigate the change of clostridium cluster iv community in the colon of piglets from 7 to 35 days of age, and its correlation with butyrate concentration.201021141473
diversity of human colonic butyrate-producing bacteria revealed by analysis of the butyryl-coa:acetate coa-transferase gene.butyrate-producing bacteria play an important role in the human colon, supplying energy to the gut epithelium and regulating host cell responses. in order to explore the diversity and culturability of this functional group, we designed degenerate primers to amplify butyryl-coa:acetate coa-transferase sequences from faecal samples provided by 10 healthy volunteers. eighty-eight per cent of amplified sequences showed >98% dna sequence identity to coa-transferases from cultured butyrate-producing b ...201019807780
quantitative differences in intestinal faecalibacterium prausnitzii in obese indian children.gut bacteria contribute to energy conservation in man through their ability to ferment unabsorbed carbohydrate. the present study examined the composition of predominant faecal microbiota in obese and non-obese children. the participants (n 28) aged 11-14 years provided fresh faecal samples and completed a dietary survey consisting of 24 h diet recall and a ffq of commonly used foods taken over the previous 3 months. faecal bacteria were quantitated by real-time pcr using primers targeted at 16s ...201019849869
cerebral perfusion pressure: a hemodynamic mechanism of mannitol and the postmannitol hemogram.sixteen patients each received infusions of 1 g of mannitol per kg over 5 to 10 minutes, and serial determinations of intracranial pressure (icp), systemic arterial blood pressure (sabp), central venous pressure, cerebral perfusion pressure (cpp), hematocrit, hemoglobin, serum na+, k+, osmolarity, and fluid balance were carried out for 4 hours. urine output was replaced volume for volume with 5% dextrose in 0.45% nacl solution. we tested the hypothesis that patients with high (greater than or eq ...20103116451
initiation of swimming activity by trigger neurons in the leech subesophageal ganglion. iii. sensory inputs to tr1 and tr2.in papers i and ii of this series, we described two pairs of interneurons, tr1 and tr2, in the leech subesophageal ganglion which can trigger swimming activity in the isolated central nervous system (cns). in this paper, we describe sensory inputs to these trigger neurons from previously identified mechanosensory neurons. we found that: weak mechanical stimulation (stroking) of a body wall flap attached to a segmental ganglion in an otherwise isolated cns excites the contralateral tr1 slightly. ...20103023604
mineral retention in young men consuming soy-fiber-augmented liquid-formula diets.the effect of a nutritionally complete liquid-formula diet without soy polysaccharide or with the fiber source at 20, 30, or 40 g/d on mineral retention in young men was examined. addition of 20 g soy polysaccharide to the liquid formula significantly (p less than 0.05) improved the retentions of copper, iron, zinc, and magnesium over the diet without added fiber. the highest level of soy polysaccharide (40 g/d) resulted in significantly (p less than 0.05) lower cu, zn, fe, and mg retentions tha ...20102841841
allergy to diphenylamine from an industrial grease. 20102940051
free and sulfoconjugated catecholamines in normotensive uremic patients: effects of hemodialysis.in 9 normotensive uremic patients undergoing chronic hemodialysis, the baseline plasma catecholamines varied widely from low-normal to very high; sulfoconjugated plasma catecholamines were constantly high. a dialysis-induced fall of all sulfated catecholamines and epinephrine was observed. norepinephrine decreased in 5 patients and increased in 4, with a strong inverse correlation between predialysis norepinephrine and delta norepinephrine (p less than 0.0001). no correlation was evident between ...20102918946
[encapsulating peritonitis in periodic disease. apropos of a case studied by x-ray computed tomography].a case of encapsulating peritonitis complicating the course of familial mediterranean fever is reported. this encapsulating peritonitis was responsible for abdominal pain and had a "pseudocystic" appearance on ultrasonography and computed tomography. ultrasound guided aspiration produced a yellowish fluid rich in proteins and poor in cells. surgical operation revealed a congested appearance of the peritoneum associated with a richly fibrinous appearance of the external wall of the mass. the diff ...20102817728
liquid chromatographic profiles of major carotenoid esters in commercially processed california navel and valencia orange juice concentrates.a procedure for establishing profiles of major carotenoid esters in commercially processed valencia and navel orange juice concentrates by reversed-phase liquid chromatography (lc) using sudan 1 as internal standard is described. the procedure involved conversion of 5,6-epoxides in heat concentrated citrus juices to more stable 5,8-epoxides by treatment of extracted carotenoids with hydrochloric acid followed by dual wavelength analyses at 400 and 465 nm using lc. the esters of 5,8-furanoids (au ...20103417817
liquid chromatographic profiles of major carotenoid esters in commercially processed california navel and valencia orange juice concentrates.a procedure for establishing profiles of major carotenoid esters in commercially processed valencia and navel orange juice concentrates by reversed-phase liquid chromatography (lc) using sudan 1 as internal standard is described. the procedure involved conversion of 5,6-epoxides in heat concentrated citrus juices to more stable 5,8-epoxides by treatment of extracted carotenoids with hydrochloric acid followed by dual wavelength analyses at 400 and 465 nm using lc. the esters of 5,8-furanoids (au ...20103417817
salvage of angioaccess after late thrombosis of radiocephalic fistulas for hemodialysis.a retrospective study of 44 surgical procedures performed in 39 patients with late occlusions of radiocephalic fistulas for hemodialysis is reported. in 6 cases (13.6%) no apparent anatomical cause was found, and a simple thrombectomy was performed as a corrective procedure. some sort of arterial and/or venous stenosis was present in the other 38 cases. in 11 cases with evidence of mild localized vascular stenosis we performed a thrombectomy and then an endoluminal angioplasty with a balloon cat ...20103733357
morphological and metastatic murine melanoma variants: motility, adhesiveness, cell surface and in vivo properties.the behaviour in vivo of tight and loose variants of murine melanoma cells is further characterized. in vitro clonal morphology is reproduced on a variety of substrates. results suggest that repeated selection of loose cells can co-select for cells with high metastatic and colonization potentials. measurement of cell motility shows that 1g3 (loose) cells are more motile than 1g8 (tight) which are restricted to movements within clonal boundaries. studies of adhesive properties show that loose cel ...20103426920
new data on the acceptance of the hepatitis b vaccine by dental personnel in the united kingdom. 20102964251
p21-ras effector domain mutants constructed by "cassette" mutagenesis.a series of mutations encoding single-amino-acid substitutions within the v-rash effector domain were constructed, and the ability of the mutants to induce focal transformation of nih 3t3 cells was studied. the mutations, which spanned codons 32 to 40, were made by a "cassette" mutagenesis technique that involved replacing this portion of the v-rash effector domain with a linker carrying two bspmi sites in opposite orientations. since bspmi cleaves outside its recognition sequence, bspmi digesti ...20103062384
contact urticaria to chlorocresol. 20102948766
infective endocarditis and antibiotic prophylaxis failure following an endodontic procedure. 20103063597
infective endocarditis and antibiotic prophylaxis failure following an endodontic procedure. 20103063597
[end-to-side gastroduodenal anastomosis (author's transl)]. 20104618314
cancer cachexia.cancer cachexia describes a syndrome of progressive weight loss, anorexia, and persistent erosion of host body cell mass in response to a malignant growth. although often associated with preterminal patients bearing disseminated disease, cachexia may be present in the early stages of tumor growth before any signs or symptoms of malignancy. a decline in food intake relative to energy expenditure (which may be increased, normal, or decreased) is the fundamental physiologic derangement leading to c ...20103292798
[postoperative disorder of bladder emptying in gynecology: pathophysiology and possibilities for treatment].urinary retention after radical hysterectomy is due to partial or complete denervation of the bladder and proximal urethra which is dependent on surgical radicality. in contrast, incomplete bladder emptying after surgery for stress induced urinary incontinence, has its origin in an infravesicular functional obstruction and is dependent on the operation performed. the use of drugs (alpha-sympatholytics, parasympathomimetics) seem to be of limited value, especially after stress-incontinence surger ...20103063586
[postoperative disorder of bladder emptying in gynecology: pathophysiology and possibilities for treatment].urinary retention after radical hysterectomy is due to partial or complete denervation of the bladder and proximal urethra which is dependent on surgical radicality. in contrast, incomplete bladder emptying after surgery for stress induced urinary incontinence, has its origin in an infravesicular functional obstruction and is dependent on the operation performed. the use of drugs (alpha-sympatholytics, parasympathomimetics) seem to be of limited value, especially after stress-incontinence surger ...20103063586
[immunologic support for clinical bone marrow transplants]. 20103063589
[immunologic support for clinical bone marrow transplants]. 20103063589
lipid solubility of a series of drugs and its relevance to fatal poisoning.for 11 commonly used drugs, the n-octanol/water partition coefficient (ph 7.4 temperature 37 degrees c) and solubility in n-octanol were determined. the drugs tested were chlorpromazine, amitriptyline, trazodone, dextropropoxyphene, diltiazem, dibucaine, amethocaine, procaine, quinidine, acetylsalicylic acid and paracetamol. for eight of the drugs, the relative lipid saturation corresponding to a fatal plasma concentration was estimated from the two parameters determined above and the median fat ...20102897446
effect of salt depletion on sodium concentration in serum and urine of bufo chilensis. evidences for increased levels of neurohypophysial principles in their plasma.salt-depleted toads bufo chilensis were compared with animals maintained in nacl solution and a control group with respect to na+ content in serum and urine. plasma hydro-osmotic activity of the animals was measured by increased water transfer across the isolated urinary bladder of the frog (caudiverbera caudiverbera). sodium in serum is not affected by pre-adaptation in distilled water. urine na+ is markedly reduced. plasma from depleted animals increases water transfer across the isolated urin ...20102881665
relationship between hepatitis b surface antigen prevalence, per capita alcohol consumption and primary liver cancer death rate in 30 countries.the relationships between the prevalence of hepatitis b surface antigen (hbsag), mean annual per capita alcohol consumption and primary liver cancer (plc) death rates were explored in 30 countries. hbsag prevalence was associated, significantly, with the logarithm of the primary liver cancer death rate (simple correlation coefficient = 0.44, p less than 0.05). this significant association increased following adjustment for a country's mean annual per capita alcohol consumption (partial correlati ...20102843610
[bibliography of scientific papers of the charles' university medical school at hradec králové]. 20104620040
the physiology and pathophysiology of eicosanoids in the skin.eicosanoids may play a role in the pathophysiology of common inflammatory skin diseases such as psoriasis and atopic eczema. their involvement offers the hope for development of new antiinflammatory drugs for use in dermatopharmacology. on the other hand, new findings also suggest a tissue protective role of prostanoids in skin. in addition, physiological functions of lipoxygenase-derived eicosanoids are emerging, which may serve to maintain cutaneous integrity and restore normal skin structure ...20103152488
duplex doppler evaluation of renal allograft dysfunction. 20092652615
clinicopathologic spectrum of cutaneous manifestations in systemic follicular lymphoma. a study of 11 patients.follicular lymphoma is rarely diagnosed on the basis of only a cutaneous biopsy. eleven patients with primary follicular lymphoma of lymph nodes who developed skin lesions were studied. skin lesions involved the scalp, head and neck, trunk, and buttocks and were small cleaved cell type in four cases, large cell type in three cases, and mixed type in four cases. the follicular pattern with a bottom-heavy distribution was identified in only six of the 11 cases. one case showed a nodular, perivascu ...20092789095
[verapamil in primary hyperparathyroidism].in a female patient with primary hyperparathyroidism and disturbances of cardiovascular function clinical, biochemical and electrocardiographic as well as bone scintigraphic parameters were analyzed before and during therapy with verapamil (falicard) for 7 month. verapamil therapy resulted in decrease of the frequency of the supraventricular tachycardia, and, in higher doses (4 x 120 mg), also reduction of blood pressure, however, with dose limiting bradycardia and prolongation of pq-time. both ...20092763597
prevention of leprosy. 20094108289
the microbiota in inflammatory bowel disease in different age groups.many efforts were made in the past decades to assess the role of gut microbiota in inflammatory bowel diseases (ibd), leading to the hypothesis that an altered microbial composition, other than the presence of a specific pathogen, could be involved in the pathogenesis of the disease. on the other hand, existing differences in gut microbial community between distinct classes of age make sense of an increasing research in microbial shifts in ibd.200919786749
diversity, metabolism and microbial ecology of butyrate-producing bacteria from the human large intestine.butyrate-producing bacteria play a key role in colonic health in humans. this review provides an overview of the current knowledge of the diversity, metabolism and microbial ecology of this functionally important group of bacteria. human colonic butyrate producers are gram-positive firmicutes, but are phylogenetically diverse, with the two most abundant groups related to eubacterium rectale/roseburia spp. and to faecalibacterium prausnitzii. five different arrangements have been identified for t ...200919222573
low counts of faecalibacterium prausnitzii in colitis microbiota.the intestinal microbiota is suspected to play a role in colitis and particularly in inflammatory bowel disease (ibd) pathogenesis. the aim was to compare the fecal microbiota composition of patients with colitis to that of healthy subjects (hs).200919235886
commensal bacteria, traditional and opportunistic pathogens, dysbiosis and bacterial killing in inflammatory bowel diseases.the authors present evidence published during the past 2 years of the roles of commensal and pathogenic bacteria in the pathogenesis of the inflammatory bowel diseases.200919352175
effects of a gluten-free diet on gut microbiota and immune function in healthy adult human subjects.diet influences the composition of the gut microbiota and host's health, particularly in patients suffering from food-related diseases. coeliac disease (cd) is a permanent intolerance to cereal gluten proteins and the only therapy for the patients is to adhere to a life-long gluten-free diet (gfd). in the present preliminary study, the effects of a gfd on the composition and immune function of the gut microbiota were analysed in ten healthy subjects (mean age 30.3 years) over 1 month. faecal mic ...200919445821
bioavailability of hydroxychloroquine tablets in healthy volunteers.1. five healthy volunteers received, in a randomised crossover design study, a 155 mg oral tablet and an intravenous infusion of 155 mg racemic hydroxychloroquine (200 mg hydroxychloroquine sulphate) to assess the bioavailability of the commercially available tablet (plaquenil, winthrop laboratories, australia). 2. the terminal elimination half-life of hydroxychloroquine is more than 40 days, thus blood and urine samples were collected for 5 months following each dose to characterise adequately ...20092757893
clinical significance of the immunological tests in rheumatoid arthritis.of the many theoretical causes of rheumatoid arthritis(ra), the most widely held theory is the autoimmune mechanism. in order to clarify the clinical significance of the immunological tests in ra, we studied immunoglobulin and complement levels in sera and synovial fluids of 118 ra patients and the following results were obtained. 1) the levels of immunoglobulins were elevated in both serum and synovial fluid and this was more prominent in the seropositive cases than the seronegative ones. 2) th ...20092741472
effect of inulin on the human gut microbiota: stimulation of bifidobacterium adolescentis and faecalibacterium prausnitzii.prebiotics are food ingredients that improve health by modulating the colonic microbiota. the bifidogenic effect of the prebiotic inulin is well established; however, it remains unclear which species of bifidobacterium are stimulated in vivo and whether bacterial groups other than lactic acid bacteria are affected by inulin consumption. changes in the faecal microbiota composition were examined by real-time pcr in twelve human volunteers after ingestion of inulin (10 g/d) for a 16-d period in co ...200918590586
twin studies reveal specific imbalances in the mucosa-associated microbiota of patients with ileal crohn's disease.large interindividual variation in the composition of the intestinal microbiota between unrelated individuals has made it challenging to identify specific aspects of dysbiosis that lead to crohn's disease (cd).200919023901
nutrition studies of ugandan refugees in sudan and rwanda. a report on surveys using the mid-upper arm circumference method.nutritional surveys were undertaken in refugee camps in southern sudan and rwanda. nutrition status was assessed using the mid-upper arm circumference method. a high level of malnutrition was found in the main transit camp for refugees from uganda into southern sudan. nutrition status in refugee settlements was adequate in the rwanda camps, where refugees had only been displaced for a short period of time; there was only a limited degree of malnutrition. the findings correlate with the time peri ...20094095778
[use of the kimray-greenfield filter in the prevention of pulmonary embolism. personal experience].personal experience in the use of intra-caval filters in the prevention of pulmonary embolisms is reported. the advantages and the indications for positioning of the k.g. filter are analysed on the basis of a series of 31 patients operated on with this method.20092812457
[use of the kimray-greenfield filter in the prevention of pulmonary embolism. personal experience].personal experience in the use of intra-caval filters in the prevention of pulmonary embolisms is reported. the advantages and the indications for positioning of the k.g. filter are analysed on the basis of a series of 31 patients operated on with this method.20092812457
[the rehabilitation phase of hand surgery]. 20092748164
when to use posts. 20092698331
differing responses in right and left ventricular filling, loading and volumes during positive end-expiratory pressure.using a combined hemodynamic and radionuclide technique, 20 patients with varied ventricular function were evaluated during positive end-expiratory pressure (peep) application. left ventricular (lv) and right ventricular (rv) ejection fractions and cardiac output were measured, and ventricular volumes were derived. seven patients (group 1) who had an increase in lv end-diastolic volume with peep and 13 patients (group 2) who had the more typical response, a decrease in lv end-diastolic volume wi ...20092679024
myofascial pain dysfunction and viola playing.two cases are illustrated to demonstrate the importance of occupation, in the form of viola playing, in the aetiology of myofascial pain dysfunction. an understanding of the contribution of tension and playing style, together with physical limitations, is emphasised and, consequently, it is acknowledged that modification may be necessary to the formulation of a treatment plan.20092713183
[quadrantectomy. choice therapy for 23 clinical cases of t1 carcinoma in situ of the breast]. 20093774180
[quadrantectomy. choice therapy for 23 clinical cases of t1 carcinoma in situ of the breast]. 20093774180
quality assurance: public hospital clinics. 20092738944
[magnetic resonance and computed tomography in brain evaluation]. 20092920680
differential regulation of oncogenic and cellular p185 by serine/threonine kinases.185c-neu is a member of a family of growth factor receptors with tyrosine kinase activity. a point mutation in the transmembrane region leads to activation of the enzymatic domain. we demonstrate that tpa (phorbol-12-myristate-13-acetate) stimulates the phosphorylation of p185c-neu on serine and threonine residues coincident with the inhibition of its intrinsic tyrosine kinase and the proliferation of cells that express it. the tyrosine kinase activity as well as the phosphorylation pattern of s ...20082575488
infective endocarditis in children. a seven-year experience.eighteen children with infective endocarditis were detected over seven years. fever, gastrointestinal and chest symptoms were striking features. anaemia, elevated leukocyte count and sedimentation rate were constant laboratory findings. strep. viridans and staph. aureus were the commonest organisms isolated. we concluded that rheumatic and congenital heart disease are equally important in predisposition to infective endocarditis.20082519331
mechanics of the lung and chest wall. 20082291386
[percutaneous drainage of fistula-associated abscesses and biliary fistulas].33 abdominal abscesses associated with fistulae in 31 patients were treated by percutaneous drainage. 19 of these patients had had surgery immediately preceding the drainage. in 64% the percutaneous drainage led to a diagnosis of an internal fistula. additional therapeutic measures, because of the fistula, were necessary in 45% (operation, biliary drainage, repositioning of catheter). the average duration of drainage was 29 days. 77% of those abscesses which could be drained were treated success ...20082538887
bacterial succession in the colon during childhood and adolescence: molecular studies in a southern indian village.the colonic bacterial flora, largely anaerobic, is believed to establish and stabilize in the first 2 y of life.200819064526
characterization of cecal microbiota and response to an orally administered lactobacillus probiotic strain in the broiler chicken.a probiotic lactobacillus strain was given in drinking water to young broiler chickens from 1 to 19 days of age. cecal contents were collected from 4- and 19-day-old chickens in treated and control groups. enumeration of bacteria by culture on selective media showed a decrease in clostridium perfringens carriage in the 4-day-old treated chickens, whereas coliforms and lactobacillus populations were not significantly affected by the treatment. fluorescent in situ hybridization analysis with 7 phy ...200817957118
real-time polymerase chain reaction quantification of specific butyrate-producing bacteria, desulfovibrio and enterococcus faecalis in the feces of patients with colorectal cancer.bacterial metabolites produced in the bowel are potentially related to the genesis of colorectal cancer. butyrate is protective against cancer, whereas hydrogen sulfide and oxygen free radicals can be toxic to the epithelium. the present study was designed to quantitate eubacterium rectale, faecalibacterium prausnitzii (both butyrate-producing bacteria), desulfovibrio (sulfate-reducing bacteria), and enterococcus faecalis (that produces extracellular superoxide) in the feces of patients with col ...200818624900
cloning and sequencing of the gene for cellobiose 2-epimerase from a ruminal strain of eubacterium cellulosolvens.cellobiose 2-epimerase (ce; ec 5.1.3.11) is known to catalyze the reversible epimerization of cellobiose to 4-o-beta-d-glucopyranosyl-d-mannose in ruminococcus albus cells. here, we report a ce in a ruminal strain of eubacterium cellulosolvens for the first time. the nucleotide sequence of the ce had an orf of 1218 bp (405 amino acids; 46 963.3 da). the ce from e. cellulosolvens showed 44-54% identity to n-acyl-d-glucosamine 2-epimerase-like hypothetical proteins in the genomes of coprococcus eu ...200818710396
faecalibacterium prausnitzii is an anti-inflammatory commensal bacterium identified by gut microbiota analysis of crohn disease patients.a decrease in the abundance and biodiversity of intestinal bacteria within the dominant phylum firmicutes has been observed repeatedly in crohn disease (cd) patients. in this study, we determined the composition of the mucosa-associated microbiota of cd patients at the time of surgical resection and 6 months later using fish analysis. we found that a reduction of a major member of firmicutes, faecalibacterium prausnitzii, is associated with a higher risk of postoperative recurrence of ileal cd. ...200818936492
active crohn's disease and ulcerative colitis can be specifically diagnosed and monitored based on the biostructure of the fecal flora.the intestinal microflora is important in the pathogenesis of inflammatory bowel disease (ibd). the impact of its spatial organization on health and disease is unknown.200818050295
symbiotic gut microbes modulate human metabolic phenotypes.humans have evolved intimate symbiotic relationships with a consortium of gut microbes (microbiome) and individual variations in the microbiome influence host health, may be implicated in disease etiology, and affect drug metabolism, toxicity, and efficacy. however, the molecular basis of these microbe-host interactions and the roles of individual bacterial species are obscure. we now demonstrate a"transgenomic" approach to link gut microbiome and metabolic phenotype (metabotype) variation. we h ...200818252821
detection of blastocystis from stool samples using real-time pcr.we developed a real-time lc pcr assay to detect a 152 bp sequence in an uncharacterized region of the blastocystis genome. the described assay detected 11 of 11 atcc strains of blastocystis from subtypes 1, 3, and 4. three of three stool samples from oregon and california military personnel that were negative for blastocystis by an ova and parasite test as well as a conventional pcr assay were positive for blastocystis using our real-time lc pcr assay. diagnosis of blastocystis infections using ...200818488250
molecular analysis of the digestive microbiota in a gnotobiotic mouse model during antibiotic treatment: influence of saccharomyces boulardii.the probiotic saccharomyces boulardii is a non-pathogenic yeast that has been proven efficient in the prevention of antimicrobial-associated diarrhea and of clostridium difficile associated colitis. we evaluated the influence of the administration of s. boulardii on the composition of the fecal microbiota in a human microbiota-associated mouse model. this evaluation was run before, during and after a 7-day oral treatment with amoxicillin clavulanic acid. predominant groups of bacteria were quant ...200818511310
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