Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| [bacteria isolated from perforation peritonitis and their antimicrobial susceptibilities]. | bacteria isolated from 25 cases of perforation peritonitis and its antimicrobial susceptibilities were studied. one hundred and forty-six strains including 36 strains of aerobic bacteria, 107 strains of anaerobic bacteria and 3 strains of yeast were isolated from intra-peritoneal fluid obtained at operation. among aerobic bacteria, enterobacteriaceae was isolated from 13 cases (52%) out of all, and followed by those of enterococcus spp. (24%), pseudomonas aeruginosa (20%), in this order. among n ... | 2007 | 18018418 |
| multiple pyogenic liver abscesses following hemorrhoid banding. | 2007 | 17987768 | |
| a 21-year-old man with fever and sore throat rapidly progressive to hemoptysis and respiratory failure. diagnosis: lemierre syndrome with fusobacterium necrophorum sepsis. | 2007 | 17998376 | |
| the trimeric periplasmic chaperone skp of escherichia coli forms 1:1 complexes with outer membrane proteins via hydrophobic and electrostatic interactions. | the interactions of outer membrane proteins (omps) with the periplasmic chaperone skp from escherichia coli are not well understood. we have examined the binding of skp to various omps of different origin, size, and function. these were ompa, ompg, and yaet (omp85) from escherichia coli, the translocator domain of the autotransporter nalp from neisseria meningitides, foma from fusobacterium nucleatum, and the voltage-dependent anion-selective channel, human isoform 1 (hvdac1) from mitochondria. ... | 2007 | 17928002 |
| targeted profiling of oral bacteria in human saliva and in vitro biofilms with quantitative real-time pcr. | an in vitro plaque model based on the use of human salivary bacteria and tooth-like surfaces was previously developed for studying the formation of oral biofilm and its use for pre-clinical testing of candidate antimicrobial or antiplaque agents. in this study, a quantitative taqman pcr assay (qpcr) was developed to compare the bacterial compositions of in vitro biofilms to parent saliva samples, and to determine the relative contributions of different species in the formation of the oral biofil ... | 2007 | 17653931 |
| fusobacterium: elusive cause of life-threatening septic thromboembolism. | 2007 | 17872873 | |
| clinical, bacteriological, and histopathological study of toxic puerperal metritis in iraqi buffalo. | data were collected from 42 buffalo with toxic puerperal metritis in 2 large herds, with a history of dystocia, prolapse, and retained placenta. all buffalo were subjected to detailed clinical examination including external inspection, vaginoscopy, and transrectal palpation of the cervix, uterus, and ovaries. swabs for bacteriology and biopsies for histopathology were collected from the uterine lumen from each cow. character, odor, and estimation of polymorphonuclear cells of the vaginal mucus w ... | 2007 | 17881686 |
| antimicrobial effect of triclosan and triclosan with gantrez on five common endodontic pathogens. | microbial control of the root canal system is one of the key objectives of root canal therapy. triclosan is a widely accepted broad spectrum antimicrobial agent proven to be effective against many gram-positive and gram-negative bacteria. triclosan acts by blocking bacterial fatty acid biosynthesis. the addition of gantrez copolymer has been shown to enhance the antimicrobial activity of triclosan. the purpose of this study was to determine the minimum inhibitory and bactericidal concentrations ... | 2007 | 17889698 |
| implant surface analysis and microbiologic evaluation of failed implants retrieved from smokers. | the aim of this study was to evaluate the microbiota and surface of failed titanium dental implants from 4 manufacturers. twelve mobile dental implants were retrieved from 10 smokers after 3 to 10 years of functional loading. before implant removal, microbial samples were taken and evaluated using polymerase chain reaction. after implant removal, analyses of the failed implant surfaces were performed using scanning electron microscopy and energy-dispersive spectrometer x-ray. periodontal pathoge ... | 2007 | 17912965 |
| [effects of garlic on oral bacteriostasis and root detoxification]. | to evaluate the pharmacologic effect of garlic on oral bacteriostasis and root detoxification. | 2007 | 18278414 |
| the inhibition of dentifrice containing the lotus leaf-derived inhibitor on periodontitis-related bacteria in vitro. | to search for a substance in lotus leaf that could efficiently be used in toothpaste for preventing periodontal disease. | 2007 | 17992914 |
| [alterations of salivary flow rate and oral main pathogens in miniature pig with bilateral parotid gland atrophy]. | to observe the effect of bilateral parotid gland atrophy on the whole saliva flow rate and the growth of main oral pathogens in different sites of oral cavity. | 2007 | 18476559 |
| laboratory maintenance of fusobacteria. | this unit describes routine laboratory handling of fusobacteria. different media that can be used to grow or enrich fusobacterium nucleatum and other species of this genus are described. the growth and stock conditions as well as the susceptibility of f. nucleatum to oxygen in a pure culture are also discussed. | 2006 | 18770577 |
| [antibacterial therapy of patients with complicated diabetic foot]. | the main reason for hospitalization and lethality of patients with diabetes mellitus are the infectious complications of the lower limbs. in the period 2000-2005, 147 were treated for complications with the diabetic foot in the surgical clinic at the ministry of interior medical institute. 105 of them were men and 42 - women. the average age of the patients was 67 +/- 8.2. different number of microorganisms, from 2 to 8, was identified in testing the material from the wound secret. mixed (anaero ... | 2006 | 18843904 |
| a 62-month retrospective epidemiological survey of anaerobic bacteraemia in a university hospital. | the incidence of anaerobic bacteraemia was studied retrospectively over 62 months at mont-godinne university hospital, yvoir, belgium. the distribution of organisms, clinical presentations, choice of antimicrobial therapy and clinical outcome were analysed. the proportion of positive blood cultures yielding obligate anaerobes was 3.3%. the overall incidence of clinically significant anaerobic bacteraemia was 0.51 cases/1,000 patient admissions (0.61 cases/10,000 hospital-days), but was significa ... | 2006 | 16700700 |
| inflammation, heat shock proteins and periodontal pathogens in atherosclerosis: an immunohistologic study. | inflammation is a significant component of atherosclerosis lesions. bacteria, including periodontopathogens, have been demonstrated in atherosclerotic plaques and cross-reactivity of the immune response to bacterial groel with human heat shock protein 60 has been suggested as a link between infections and atherosclerosis. | 2006 | 16842503 |
| supragingival plaque may modify the effects of subgingival bacteria on attachment loss. | bacterial interactions in response to local and systemic factors may explain important features of the pathogenesis of periodontal disease. this study evaluates the role of supragingival plaque level on the relationship between subgingival microorganisms and the risk of attachment loss. | 2006 | 16671872 |
| a model of spontaneous periodontitis in the miniature goat. | the pathogenesis and progression of periodontal disease have been extensively studied through the use of animal models. however, no animal model has yet been established that is precisely similar to periodontitis in humans. in the present study, we examined the use of shiba goats as a model for spontaneous periodontitis. | 2006 | 16671878 |
| genetic and biochemical characterization of fus-1 (oxa-85), a narrow-spectrum class d beta-lactamase from fusobacterium nucleatum subsp. polymorphum. | previous studies have reported beta-lactamase-mediated penicillin resistance in fusobacterium nucleatum, but no beta-lactamase gene has yet been identified in this species. an f. nucleatum subsp. polymorphum strain resistant to penicillin and amoxicillin was isolated from a human periodontitis sample. dna cloning and sequencing revealed a 765-bp open reading frame encoding a new class d beta-lactamase named fus-1 (oxa-85). a recombinant escherichia coli strain carrying the bla(fus-1) gene exhibi ... | 2006 | 16870757 |
| inhibitory effects of macrocarpals on the biological activity of porphyromonas gingivalis and other periodontopathic bacteria. | macrocarpals, which are phloroglucinol derivatives contained in eucalyptus leaves, exhibit antimicrobial activity against a variety of bacteria including oral bacteria. this study examined effects of macrocarpals a, b, and c on periodontopathic bacteria, especially porphyromonas gingivalis. | 2006 | 16626372 |
| response of human macrophage-like cells to stimulation by fusobacterium nucleatum ssp. nucleatum lipopolysaccharide. | monocytes/macrophages are key members of the innate immune system and are present in higher numbers in active periodontal lesions than in inactive sites. the aim of this study was to characterize the response of human monocyte u937 cells, differentiated into adherent macrophages by treatment with phorbol-12-myristate 13-acetate, to stimulation by fusobacterium nucleatum ssp. nucleatum lipopolysaccharide. attachment of (3)h-lipopolysaccharide to macrophage-like cells was partially inhibited by an ... | 2006 | 16626377 |
| hemiplegia in lemierre syndrome: a neuroimaging strategy. | 2006 | 16643325 | |
| the additional value of real-time pcr in the quantitative detection of periodontal pathogens. | for the analysis of subgingival plaque, anaerobic bacterial culture has been the gold standard for many years. currently, molecular microbial techniques have become available to identify and quantify target organisms with high specificity and sensitivity. the technique of real-time polymerase chain reaction(rt-pcr) provides a new tool to detect oral pathogens both in oral and non-oral human infections. the aim of this study was to compare the rt-pcr and anaerobic culture for detection and quanti ... | 2006 | 16677332 |
| production of indolic compounds by rumen bacteria isolated from grazing ruminants. | to screen rumen bacterial cultures and fresh ruminal isolates for indole and skatole production. | 2006 | 16696673 |
| spinal abscess and mitral valve endocarditis secondary to asymptomatic fusobacterium-induced dental abscess. | 2006 | 16816268 | |
| genetic diversity of oral fusobacterium nucleatum isolated from patients with different clinical conditions. | the genetic diversity of 23 oral fusobacterium nucleatum isolated from 15 periodontal patients, eight from seven healthy subjects, nine from nine aids patients and two from two cebus apella monkeys were analyzed. ecori restricted the bacterial dna and 28 ribotypes grouped from a to j groups were obtained. isolates formed 24 ribotypes which were contained into a, b, c, d, e and f groups, and three reference strains and two clinical isolates of a. actinomycetemcomitans, and e. coli cdc formed four ... | 2006 | 16699623 |
| evaluation of 4 swab transport systems for the recovery of atcc and clinical strains with characterized resistance mechanisms. | despite limitations of swab transport systems (sts), many clinical samples and bacterial isolates are sent on these devices. we evaluated the performance of 4 commercial sts: m40 transystem (s1, copan italia, bovezzo, italy), uni-ter (s2, meus, piove di sacco, italy), euromed (s3, lab service, surbo, italy), and eurotubo (s4, deltalab, rubí, spain). survival of atcc and clinical strains with characterized resistance mechanisms, stored at room temperature and using the roll-plate method (nccls m4 ... | 2006 | 16822635 |
| antimicrobial efficacy of medicated root canal filling materials. | bacteria remaining within the root canal system are a significant factor in endodontic failures. the aim of this in vitro study was to evaluate the antimicrobial efficacy of commercially available gutta-percha formulations against common endodontic pathogens. standard, tetracycline, or iodoform (also called medicated gutta-percha; mgp) containing gutta-percha were tested against actinomyces israelii, a. naeslundii, enterococcus faecalis, and fusobacterium nucleatum using the agar diffusion metho ... | 2006 | 16427466 |
| lemierre's syndrome: an unusual manifestation of spinal infection. | we report a case of lemierre's syndrome complicated by an epidural abscess. this manifestation of lemierre's syndrome has not been described previously. | 2006 | 16434535 |
| the changing pattern of fusobacterium infections in humans: recent experience with fusobacterium bacteraemia. | a retrospective study was conducted of 26 adult cases of fusobacterium bacteraemia that occurred between 1998 and 2003 at center hospitalier universitaire de grenoble, france. most patients presented with pre-existing adverse medical conditions, including evolving malignant diseases (eight patients), recent surgery (four patients), and chronic organ failure (six patients). only one patient presented with a classic lemierre's syndrome. these results suggest an opportunistic pattern of modern fuso ... | 2006 | 16441457 |
| index of suspicion. | 2006 | 16452277 | |
| anti-inflammatory activity of a high-molecular-weight cranberry fraction on macrophages stimulated by lipopolysaccharides from periodontopathogens. | periodontitis is a chronic inflammatory disease affecting oral tissues. the continuous, high production of cytokines by host cells triggered by periodontopathogens is thought to be responsible for the destruction of tooth-supporting tissues. macrophages play a critical role in this host inflammatory response to periodontopathogens. the aim of this study was to investigate the effect of non-dialyzable material prepared from cranberry juice concentrate on the pro-inflammatory cytokine response of ... | 2006 | 16498070 |
| coaggregation of porphyromonas gingivalis and fusobacterium nucleatum pk 1594 is mediated by capsular polysaccharide and lipopolysaccharide. | previous reports have shown that coaggregation between porphyromonas gingivalis and fusobacterium nucleatum, two important periodontopathogens, is mediated by a galactoside on the surface of p. gingivalis and a lectin on f. nucleatum. in the present study, purified capsular polysaccharide (cps) and lipopolysaccharide (lps) of p. gingivalis pk 1924 (serotype k5) were found to be able to bind to f. nucleatum cells and to inhibit binding of f. nucleatum to p. gingivalis serotype k5. sugar binding s ... | 2006 | 16499621 |
| linezolid in the treatment of brain abscess due to peptostreptococcus. | brain abscesses can be caused by bacteria, fungi, and parasites. among bacteria, anaerobic organisms include the bacteroides species group, fusobacterium, peptostreptococcus, and propionibacterium. in these cases, a 4-week course of parenteral penicillin/cefalosporin and metronidazole is the standard of treatment. we describe a case of brain abscess secondary to anaerobic infection with peptostreptococcus, which was successfully treated with parenteral and oral linezolid after failure of standar ... | 2006 | 16500781 |
| pyogenic infections of the central nervous system secondary to dental affections--a report of six cases. | pyogenic infections of the central nervous system of dental origin are quite uncommon in industrialized countries. we report six cases with intracerebral (n = 4) and intraspinal (n = 2) infections treated in our hospital. the microbial pathogen was successfully isolated in all patients. fusobacterium nucleatum as well as streptococcus species were found in three cases. bacillus species were identified in two patients. actinomyces was the etiologic agent in one case. all patients suffered from de ... | 2006 | 16501929 |
| antimicrobial susceptibility of anaerobic bacteria in new zealand: 1999-2003. | routine susceptibility testing of all anaerobic organisms is not advocated, but it is useful for laboratories to test periodically for anaerobic organisms and provide local susceptibility data to guide therapy. this study reports the national trend of antibiotic susceptibility of clinically significant anaerobes in new zealand. | 2006 | 16507560 |
| the microbiological origin of fluorescence observed in plaque on dentures during qlf analysis. | the aim of this study was to determine the microbiological origin of plaque fluorescence observed during quantitative light-induced fluorescence (qlf) analysis. plaque was sampled from dentures, because of easy accessibility and the homogeneous background provided by the denture tooth during imaging, and the acknowledged comparability to occlusal plaque. forty removable poly(methyl methacrylate) dentures were screened for the presence of fluorescent plaque deposits during qlf analysis. dentures ... | 2006 | 16508267 |
| effects of streptococcus mutans gtfc deficiency on mixed oral biofilms in vitro. | the aim of this study was to examine the influence of glucosyltransferase-gene-negative (gtf-) streptococcus mutans strains unable to synthesize water-insoluble or soluble glucan on the structure and macromolecular diffusion properties of in vitro grown mixed oral biofilms. biofilms modeling supragingival plaque consisted of actinomyces naeslundii omz 745, candida albicans omz 110, fusobacterium nucleatum kp-f2, streptococcus oralis sk 248, veillonella dispar atcc 17748t and one of the s. mutans ... | 2006 | 16508276 |
| the role of anaerobic bacteria in mediastinitis. | the management of mediastinitis involves directing appropriate antibacterial therapy against the potential bacterial pathogens. the increased recovery of anaerobic bacteria from mediastinal infections has led to a greater appreciation of their role in this condition and to re-evaluation of the proper treatment of this condition. mediastinitis caused by anaerobic bacteria generally emerges following perforation of the oesophagus, extension of retropharyngeal abscess, suppurative parotitis, cervic ... | 2006 | 16526820 |
| factors affecting lysine degradation by ruminal fusobacteria. | fusobacterium necrophorum can readily be enriched from the rumen with lysine, and its deamination rate is very rapid. the addition of f. necrophorum jb2 to mixed ruminal bacteria significantly increased lysine degradation, but only if the ratio of ruminal fluid to basal medium was less than 25%. if more ruminal fluid (ph 6.1) was added, ammonia production decreased by as much as 80%. clarified, autoclaved ruminal fluid was also inhibitory. when f. necrophorum jb2 was grown in a lysine-limited co ... | 2006 | 16542401 |
| necrobacillosis without lemierre's syndrome. | 2006 | 16714193 | |
| autoaggregation and coaggregation of bacteria associated with acute endodontic infections. | biofilms and microbial aggregates are a common mechanism for the survival of bacteria in nature. microbial aggregates have been associated with intraradicular and extraradicular endodontic disease. one objective of this study was to assess bacteria isolated from acute endodontic infections for autoaggregation and coaggregation. another objective was to use both a conventional visual assay and a novel fluorescent dye-staining technique to study bacterial aggregation. sixty-two strains of bacteria ... | 2006 | 16554201 |
| folding kinetics of the outer membrane proteins ompa and foma into phospholipid bilayers. | the folding mechanism of outer membrane proteins (omps) of gram-negative bacteria into lipid bilayers has been studied using ompa of e. coli and foma of f. nucleatum as examples. both, ompa and foma are soluble in unfolded form in urea and insert and fold into phospholipid bilayers upon strong dilution of the denaturant urea. ompa is a structural protein and forms a small ion channel, composed of an 8-stranded transmembrane beta-barrel domain. foma is a voltage-dependent porin, predicted to form ... | 2006 | 16581049 |
| protective effects of grape seed proanthocyanidins against oxidative stress induced by lipopolysaccharides of periodontopathogens. | during phagocytosis or stimulation with bacterial components, macrophages activate various cell processes, including the production of reactive oxygen species (ros) and reactive nitrogen species (rns), which are critical for successful defense against invading organisms. increased levels of ros/rns create oxidative stress that results in tissue and bone destruction. grape seed proanthocyanidins have been reported to possess a wide range of biologic properties against oxidative stress. in the pre ... | 2006 | 16881806 |
| the role of anaerobic bacteria in sinusitis. | the normal oropharyngeal flora contained aerobic and anaerobic bacteria that can cause respiratory infections including sinusitis. some of these bacteria can interfere with the growth of potential pathogens and may play a role in preventing infections. anaerobic bacteria emerge as pathogens as the infection becomes chronic. this may be the result of the selective pressure of antimicrobial agents that enable resistant anaerobic organisms to survive, and from the development over time of condition ... | 2006 | 16701606 |
| lemierre's syndrome: still an important clinical entity. | we report a case of lemierre's syndrome caused by fusobacterium necrophorum and discuss characteristics of this potentially fatal condition which, though rare, may have a rising frequency. familiarity with the signs and symptoms of lemierre's syndrome is therefore of great importance. | 2006 | 16709530 |
| [in vitro antimicrobial susceptibility of anaerobic bacteria isolated from pleuropulmonary infections]. | aspirative pleuropulmonary infections are usually caused by anaerobic flora of the mouth, mainly prevotella, fusobacterium and peptostreptococcus spp. penicillin in high doses is the traditional treatment for this type of infections but the rising resistance developed in recent years has induced the empiric use of clindamycin, increasing treatment costs. | 2006 | 16758082 |
| from a sore throat to the intensive care unit: the lemierre syndrome. | lemierre syndrome is characterized by an acute oropharyngeal infection, suppurative thrombophlebitis of the internal jugular vein and metastatic infections. the infection is usually caused by fusobacterium necrophorum. we report on a 19-year-old male patient who was admitted with a five-day history of fever, sore throat and progressive dyspnea. computed tomography of the neck and chest revealed a parapharyngeal abscess, jugular vein thrombosis, descending necrotizing mediastinitis and multiple a ... | 2006 | 16794763 |
| in vitro activity of linezolid against clinical isolates of fusobacterium spp. | although most susceptibility studies for linezolid have investigated aerobic bacteria, only a few have investigated anaerobe isolates. the aim of the present study was to determine the antibacterial activity of linezolid against a larger sample of clinical isolates of fusobacterium spp. and to report on the detailed susceptibility, stratified by species. | 2006 | 16905529 |
| state-dependent conformations of the translocation pathway in the tyrosine transporter tyt1, a novel neurotransmitter:sodium symporter from fusobacterium nucleatum. | the gene of a novel prokaryotic member (tyt1) of the neurotransmitter:sodium symporter (nss) family has been cloned from fusobacterium nucleatum. in contrast to eukaryotic and some prokaryotic nsss, which contain 12 transmembrane domains (tms), tyt1 contains only 11 tms, a characteristic shared by approximately 70% of prokaryotic nss homologues. nonetheless upon heterologous expression in an engineered escherichia coli host, tyt1 catalyzes robust na+-dependent, highly selective l-tyrosine transp ... | 2006 | 16798738 |
| influence of residual bacteria on periapical tissue healing after chemomechanical treatment and root filling of experimentally infected monkey teeth. | the purpose of this study was twofold: first, to determine the influence on the healing of the periapical tissues when selected bacterial strains and combinations thereof remain after root canal treatment; and, second, the relationship to healing of the quality of the root filling. in eight monkeys, 175 root canals, previously infected with combinations of four or five bacterial strains and with radiographically verified apical periodontitis, were endodontically treated, bacteriologically contro ... | 2006 | 16911098 |
| antimicrobial actions of benzimidazoles against the oral anaerobes fusobacterium nucleatum and prevotella intermedia. | benzimidazoles are widely used as proton-pump inhibitors to control stomach hyperacidity and have been found also to have antimicrobial actions against helicobacter pylori and oral streptococci. our primary aim was to determine if they are active also against oral anaerobes associated with gingivitis. our major focus was on catabolism because it leads to production of inflammatory metabolites such as butyrate and ammonia. the benzimidazoles are effective in the protonated form at acid ph values ... | 2006 | 16806047 |
| potentiation of the bactericidal activity of harungana madagascariensis lam. ex poir. (hypericaceae) leaf extract against oral bacteria using poly (d, l-lactide-co-glycolide) nanoparticles: in vitro study. | harungana madagascariensis lam. ex poir. (hypericaceae) is known to have biological properties with mainly antibacterial, antifungal, and antiviral effects. the objective of this study was to investigate the in vitro bactericidal activity of the ethyl acetate h. madagascariensis leaf extract (hle) on the main oral bacterial strains largely implicated in dental caries and gingivitis infections, and the possibility of potentialization of hle antibacterial effects using the poly (d,l-lactide-co-gly ... | 2006 | 16809192 |
| antimicrobial barrier of an in vitro oral epithelial model. | oral epithelia function as a microbial barrier and are actively involved in recognizing and responding to bacteria. our goal was to examine a tissue engineered model of buccal epithelium for its response to oral bacteria and proinflammatory cytokines and compare the tissue responses with those of a submerged monolayer cell culture. | 2006 | 16815238 |
| effectiveness of ultrasonic instruments in the therapy of severe periodontitis: a comparative clinical-microbiological assessment with curettes. | patients with deep periodontal pockets were treated with either vector system (tg) or manual instruments (cg). clinical assessments by supragingival plaque (pl+), gingival index (gi), bleeding on probing (bop), probing depth (pd), clinical attachment level (cal) and subgingival plaque collection for microbiological analysis were made prior to and after treatment. multiplex polymerase chain reaction was used to determine the presence of actinobacillus actinomycetemcomitans, campylobacter rectus, ... | 2006 | 16841550 |
| antibacterial effect of electrolyzed water on oral bacteria. | this study investigated the antibacterial effect of electrolyzed water on oral bacteria both in vitro and in vivo. tap water was electrolyzed in a water vessel using platinum cell technology. the electrolyzed tap water (called puri-water) was put in contact with five major periodontopathogens or toothbrushes contaminated with these bacteria for 30 sec. in addition, puri-water was used as a mouthwash for 30 sec in 16 subjects and the antibacterial effect on salivary bacteria was evaluated. puri-w ... | 2006 | 16953177 |
| [inhibition of extracts from 17 chinese herbs on periodontal pathogenic microbes]. | to evaluate the efficiency of 17 chinese herbs on periodontal pathogenic microbes. | 2006 | 16955176 |
| lemierre's syndrome and genetic polymorphisms: a case report. | lemierre's syndrome presents a classic clinical picture, the pathophysiology of which remains obscure. attempts have been made to trace genetic predispositions that modify the host detection of pathogen or the resultant systemic reaction. | 2006 | 16846490 |
| composition of the bacterial flora in tonsilloliths. | tonsilloliths are a potential cause of oral malodor. in this study, microbial profiles and composition of tonsilloliths were determined using culture-independent molecular methods and scanning electron microscopy. 16s ribosomal rna bacterial genes (16s rdnas) isolated from tonsilloliths of 6 individuals were amplified by pcr and cloned into escherichia coli. partial 16s rdna sequences of approximately 600 bases of cloned inserts were used to determine species identity by comparison with sequence ... | 2006 | 16859950 |
| changes in the subgingival microbiota and periodontal parameters before and 3 months after bracket placement. | dental plaque is organized in a biofilm complex that provides protection and nutrients for periodontopathic bacteria. several factors can affect microbial colonization, including restorations and orthodontic brackets. the aim of this study was to investigate changes in subgingival microbiota and clinical parameters before and after bracket placement. | 2006 | 16979483 |
| effects of feeding polyclonal antibody preparations on ruminal bacterial populations and ruminal ph of steers fed high-grain diets. | three experiments with factorial arrangements of treatments were designed to test the efficacy of avian-derived polyclonal antibody preparations (pap) against streptococcus bovis (pap-sb) or fusobacterium necrophorum (pap-fn) in reducing ruminal counts of target bacteria in beef steers supplemented or not with feed additives (300 mg of monensin/d and 90 mg of tylosin/d; mt). feeding increasing doses of pap-sb in exp. 1 or a single dose in exp. 2 reduced s. bovis counts in a cubic fashion (p = 0. ... | 2006 | 16864880 |
| an in vitro study of antimicrobial activity and efficacy of iodine-generating hydrogel dressings. | to determine the antimicrobial activity and efficacy of different formulations of novel bioxygenating hydrogel dressings (which deliver both iodine and oxygen into the wound) against various target organisms by means of an in vitro test system that more effectively mimics the conditions encountered when dressings are in contact with wounds. | 2006 | 16869198 |
| [plaut-vincent's acute necrotizing ulcerative fusospirillum tonsillitis associated to infectious mononucleosis]. | we report the case of a 14 years old girl patient with recurrent tonsillitis who had been diagnosed and admitted previously with plaut-vincent's angina. six weeks after leaving the hospital she was assited in emergencies by an infectious mononucleosis associated to unilateral tonsillar necrotizing ulceration from which a sample was taken to microbiological exam finding the fusospirochaetal complex borrelia vincenti and fusobacterium necrophorum. a literature review at the respect of such associa ... | 2006 | 16881548 |
| root canal microbiota of dogs' teeth with periapical lesions induced by two different methods. | the microbial composition was investigated in root canals of dogs' teeth with periapical lesions induced by 2 different methods: open versus sealed canals. | 2006 | 16997126 |
| characterization of proteolytic activities of fusobacterium nucleatum. | this investigation attempted to detect the proteolytic activity of fusobacterium nucleatum in living cells, lysate cells, and supernatant of cultures. the reactions were optimized in their ph, temperature, reaction time, enzyme source, and substrate volume. synthetic substrates beta-naphthylamides (cys-na, ser-na, leu-na, glu-na, lys-na and bana), carbobenzoxy l-tirosine p-nitrophenylester (ctn), and natural substrate azoalbumin were used. reaction occurred with cys-na, ser-na, and glu-na in liv ... | 2006 | 16728241 |
| fusobacterium necrophorum determined as abortifacient in sheep by laser capture microdissection and fluorescence in situ hybridization. | fluorescent in situ hybridization (fish) has been extensively used for identification of individual microbial cells within their natural environment. the present work describes the identification of fusobacterium necrophorum in formalin-fixed tissue samples from three sets of ovine twins aborted at late pregnancy by a technique that combines laser capture microdissection (lcm) and fluorescent in situ hybridization (lcm-fish). cultural bacteriological examination had failed to identify an infecti ... | 2006 | 16737796 |
| streptobacillus moniliformis endocarditis. | 2006 | 16752478 | |
| bacteriology of chronic sinusitis and acute exacerbation of chronic sinusitis. | to establish the microbiological characteristics of acute exacerbation of chronic sinusitis (aecs). | 2006 | 17043258 |
| efficient gene transfer and targeted mutagenesis in fusobacterium nucleatum. | fusobacterium nucleatum is a gram-negative anaerobe important in dental biofilm ecology and infectious diseases with significant societal impact. the lack of efficient genetic systems has hampered molecular analyses in this microorganism. we previously reported construction of a shuttle plasmid, phs17, using the native fusobacterial plasmid pfn1 and an erythromycin resistance cassette. however, the host range of phs17 was restricted to f. nucleatum, atcc 10953, and the transformation efficiency ... | 2006 | 16115683 |
| immediate effect of instrumentation on the subgingival microflora in deep inflamed pockets under strict plaque control. | to investigate (1) reduction in the number of microorganisms obtained directly after subgingival instrumentation, (2) rate of bacterial re-colonization during 2 weeks, under supragingival plaque-free conditions. | 2006 | 16367855 |
| fusobacterium nucleatum transports noninvasive streptococcus cristatus into human epithelial cells. | analysis of human buccal epithelial cells frequently reveals an intracellular polymicrobial consortium of bacteria. although several oral bacteria have been demonstrated to invade cultured epithelial cells, several others appear unable to internalize. we hypothesized that normally noninvasive bacteria may gain entry into epithelial cells via adhesion to invasive bacteria. fusobacterium nucleatum is capable of binding to and invading oral epithelial cells. by contrast, streptococcus cristatus bin ... | 2006 | 16369022 |
| antibacterial action of photoactivated disinfection {pad} used on endodontic bacteria in planktonic suspension and in artificial and human root canals. | to measure antibacterial action of photoactivated disinfection (pad) on endodontic bacteria in planktonic suspension and root canals. methods: four bacteria, fusobacterium nucleatum,peptostreptococcus micros, prevotella intermedia and streptococcus intermedius, were tested in suspension. after mixing equal volumes of tolonium chloride and bacterial suspension for 60s, each 200 microl of concentration (>10(6)cfu ml(-1)) was irradiated with light at 633+/-2 nm. each energy dose/tolonium chloride c ... | 2006 | 16239058 |
| the two major subspecies of fusobacterium necrophorum have distinct leukotoxin operon promoter regions. | fusobacterium necrophorum, a gram-negative, non-spore-forming anaerobe, is a normal inhabitant of the alimentary tract of animals and humans. two types of f. necrophorum, subspecies necrophorum (biotype a) and funduliforme (biotype b), have been recognized, which differ morphologically, biochemically and biologically. the organism is an opportunistic pathogen that causes numerous necrotic conditions (necrobacillosis) such as bovine hepatic abscesses and ruminant foot abscesses. subspecies necrop ... | 2006 | 16303263 |
| the major outer membrane protein of fusobacterium nucleatum (foma) folds and inserts into lipid bilayers via parallel folding pathways. | membrane protein insertion and folding was studied for the major outer membrane protein of fusobacterium nucleatum (foma), which is a voltage-dependent general diffusion porin. the transmembrane domain of foma forms a beta-barrel that is predicted to consist of 14 beta-strands. here, unfolded foma is shown to insert and fold spontaneously and quantitatively into phospholipid bilayers upon dilution of the denaturant urea, which was shown previously only for outer membrane protein a (ompa) of esch ... | 2006 | 16310217 |
| prevotella bivia necrobacillosis following infectious mononucleosis. | a case of lemierre's syndrome is reported. although fusobacterium species are commonly associated with this presentation, prevotella bivia was the causative micro-organism identified in this case. the finding that disseminated anaerobic sepsis followed primary ebv infection led to the construction of a hypothetical model of infection. | 2006 | 16316686 |
| inhibitory effect of weissella cibaria isolates on the production of volatile sulphur compounds. | the objective of this study was to characterize the inhibitory effects of weissella cibaria isolates on volatile sulphur compounds (vsc) production both in vitro and in vivo. | 2006 | 16489950 |
| comparison of bacterial plaque samples from titanium implant and tooth surfaces by different methods. | studies have shown similarities in the microflora between titanium implants or tooth sites when samples are taken by gingival crevicular fluid (gcf) sampling methods. the purpose of the present study was to study the microflora from curette and gcf samples using the checkerboard dna-dna hybridization method to assess the microflora of patients who had at least one oral osseo-integrated implant and who were otherwise dentate. plaque samples were taken from tooth/implant surfaces and from sulcular ... | 2006 | 16441779 |
| the ability of selected oral microorganisms to emit red fluorescence. | some novel caries detection and excavation devices rely on the ability of bacteria to produce red fluorescing compounds. the aim of this study was to examine the ability of selected oral microorganisms to emit red fluorescence. streptococcus mutans, s. oralis, s. salivarius, s. sobrinus, lactobacillus fermentans, l. casei, l. rhamnosus, actinomyces naeslundi, a. israelii, prevotella intermedia, and fusobacterium nucleatum were inoculated onto columbia agar with haemin and vitamin k and incubated ... | 2006 | 16352873 |
| endoscopically guided aerobic cultures in postsurgical patients with chronic rhinosinusitis. | there is considerable amount of debate in the literature regarding the microbial flora of normal, acutely infected, and chronically infected paranasal sinuses. few studies have specifically looked at the microbial flora of healthy and infected sinus cavities after functional endoscopic sinus surgery. | 2006 | 16539298 |
| a new checkerboard panel for testing bacterial markers in periodontal disease. | various microbiological methods have been used for testing bacterial markers for periodontitis and periodontal disease progression. most studies have used only a limited number of well recognized bacterial species. the purpose of the present study was to evaluate the association of 13 more recently identified bacterial species in a new panel in comparison with 12 previously more recognized periodontotopathogens ('old panel') using the 'checkerboard' dna-dna hybridization method. | 2006 | 16390335 |
| porphyromonas gingivalis heat shock protein vaccine reduces the alveolar bone loss induced by multiple periodontopathogenic bacteria. | heat shock protein (hsp) can be utilized as a vaccine to cross-protect against multiple pathogenic species. the present study was performed to evaluate porphyromonas gingivalis heat shock protein 60 (hsp60) as a vaccine candidate to inhibit multiple bacteria-induced alveolar bone loss. | 2006 | 16409250 |
| bilateral intraocular involvement in lemierre's syndrome. | 2006 | 16424550 | |
| [lemierre syndrome variant: hepatic abscesses and hepatic venous thrombosis due to fusobacterium nucleatum septicemia]. | like fusobacterium necrophorum, fusobacterium nucleatum is capable causing lemierre's syndrome. various locations of venous thrombosis have been described associated with fusobacterium sp. septicemia. | 2006 | 16516355 |
| unilateral empyema as a complication of infectious mononucleosis: a pathogenic variant of lemierre's syndrome. | we present a case of a healthy 19-year-old female who developed infectious mononucleosis complicated by unilateral empyema. | 2006 | 16455941 |
| genotypic and phenotypic characterization of fusobacteria from chinese and european patients with inflammatory periodontal diseases. | phylogenetic and antigenic studies were performed on 48 human oral fusobacterium strains from chinese patients with either necrotizing ulcerative gingivitis (nug) or gingivitis and on 23 fusobacterium nucleatum or fusobacterium periodonticum strains from european periodontitis patients. alignment of partial 16s rrna gene sequences resulted in a phylogenetic tree that corresponded well with the current classification of oral fusobacteria into f. periodonticum and several subspecies of f. nucleatu ... | 2006 | 16464693 |
| vertical distribution and diversity of bacteria and archaea in sulfide and methane-rich cold seep sediments located at the base of the florida escarpment. | the bacterial and archaeal communities of the sediments at the base of the florida escarpment (gulf of mexico, usa) were investigated using molecular phylogenetic analysis. the total microbial community dna of each of three vertical zones (top, middle and bottom) of a sediment core was extracted and the 16s rrna genes were amplified by pcr, cloned and sequenced. shannon-weaver diversity measures of bacteria were high in all three zones. for the archaea, diversity was generally low, but increased ... | 2006 | 16465452 |
| molecular analysis of bacteria in asymptomatic and symptomatic endodontic infections. | the purpose of the present study was to use terminal restriction fragment length polymorphism analysis and the 16s rrna gene clone library to investigate the diversity of the microbiota associated with asymptomatic and symptomatic endodontic infections and to compare the bacterial community structure in these two clinical conditions. samples were taken from asymptomatic endodontic infections associated with chronic periradicular lesions and from symptomatic infections clinically diagnosed as acu ... | 2006 | 16476021 |
| prevalence of periodontopathic bacteria on the tongue dorsum of elderly people. | the aim of this study was to analyse the prevalence of oral bacteria on the dorsum of the tongue. in addition, the relationship between the number of teeth and the microflora present on the coating of the tongue in a population of 85-year-old people was assessed. | 2006 | 16677187 |
| selective inhibition of porphyromonas gingivalis growth by a factor xa inhibitor, dx-9065a. | porphyromonas gingivalis is a causative bacterium of adult periodontitis. however, there is no drug specific for p. gingivalis and for its virulence factor. | 2006 | 16677284 |
| a sonographic short cervix as the only clinical manifestation of intra-amniotic infection. | a sonographically short cervix is a powerful predictor of spontaneous preterm delivery. however, the etiology and optimal management of a patient with a short cervix in the mid-trimester of pregnancy remain uncertain. microbial invasion of the amniotic cavity (miac) and intra-amniotic inflammation are frequently present in patients with spontaneous preterm labor or acute cervical insufficiency. this study was conducted to determine the rate of miac and intra-amniotic inflammation in patients wit ... | 2006 | 16489881 |
| effects of a collagenolytic cell wall component from fusobacterium necrophorum subsp. necrophorum on rabbit tissue-culture cells. | the effects on rabbit tissue-cultured cells of collagenolytic cell wall component (ccwc) from fusobacterium necrophorum subsp. necrophorum were investigated. scanning electron microscopy demonstrated that ccwc damaged the cell surfaces of the rabbit granulocytes and hepatocytes but the effects of the cells differed from each other. granulocytes appeared smooth and morphologically irregular whereas hepatocytes looked rough and had tiny holes in the cell membranes. differences in cell viability we ... | 2006 | 16490725 |
| the efficacy of povidone-iodine products against periodontopathic bacteria. | a total of 8 strains of 6 bacterial species, porphyromonas gingivalis atcc33277 and tdc286, actinobacillus actinomycetemcomitans atcc29523 and jp2, fusobacterium nucleatum no. 2, tannerella forsythensis atcc43937, prevotella intermedia atcc25611 and streptococcus anginosus atcc33397, were treated with povidone-iodine (pvp-i) gargle (pvp-i: 0.47 and 0.23% w/v) or chlorhexidine gluconate (chg) gargle (chg: 0.002% w/v) for 15, 30 or 60 s, after which they were inoculated into various media, culture ... | 2006 | 16490986 |
| [sore throat and a swollen neck: lemierre's syndrome until proven otherwise]. | physicians seldom prescribe antibiotics to patients presenting with a combination of sore throat and feelings of malaise. however, this restrictive regimen may have a downside. two patients, men aged 23 and 19 years, respectively, with pharyngitis developed a life-threatening syndrome following a fusobacterium throat infection. they suffered from lemierre's syndrome, referred to in recent literature as the 'forgotten disease'. the second patient recovered uneventfully after prompt intravenous an ... | 2006 | 17137089 |
| is this a variant of lemierre's syndrome? | lemierre's syndrome is an oropharyngeal infection leading to secondary septic thrombophlebitis of the internal jugular vein (ijv). it is classically anaerobic in origin and fusobacterium necrophorum is the commonest pathogen. we report an unusual variant of this condition in a 68-year-man with uncontrolled diabetes mellitus who presented with extensive cellulitis of the left neck. no primary oropharyngeal sepsis was detected. neck radiographs revealed soft tissue gas and computed tomography conf ... | 2006 | 17139410 |
| bilateral sequential petrous apicitis secondary to an anaerobic bacterium. | 2006 | 17141098 | |
| in vitro antianaerobic activity of dx-619, a new des-fluoro(6) quinolone. | the in vitro activity of dx-619, a new des-f(6) quinolone, against anaerobic bacteria was evaluated. dx-619 showed potent activity against bacteroides, prevotella, fusobacterium, micromonas, actinomyces, and clostridium spp., with mic(50)s/mic(90)s of </=0.03 to 0.25/</=0.03 to 1 microg/ml, respectively. dx-619 was also active against imipenem-resistant bacteroides spp., with mic(50)s/mic(90)s of 0.25/1 microg/ml, respectively. | 2006 | 17065629 |
| amphipathic polymers: tools to fold integral membrane proteins to their active form. | among the major obstacles to pharmacological and structural studies of integral membrane proteins (mps) are their natural scarcity and the difficulty in overproducing them in their native form. mps can be overexpressed in the non-native state as inclusion bodies, but inducing them to achieve their functional three-dimensional structure has proven to be a major challenge. we describe here the use of an amphipathic polymer, amphipol a8-35, as a novel environment that allows both beta-barrel and al ... | 2006 | 17115690 |
| effects of sonicated prevotella intermedia, fusobacterium nucleatum and lactobacillus casei extracts on interleukin-8 production by human dental pulp cells. | the objective of this study was to determine the effects of prevotella intermedia, fusobacterium nucleatum and lactobacillus casei on the production of il-8 by human dental pulp cells. human dental pulp cells from teeth of young patients (aged 18-25 years) were cultured and tested with sonicated p. intermedia atcc 25611, f. nucleatum atcc 25586 and l. casei atcc 4646 extracts. il-8 secreted into the culture supernatants were measured at 6, 12 and 24 hours using a quantitative sandwich enzyme imm ... | 2006 | 17120973 |
| common antacids could help keep gingivitis at bay. | 2006 | 17128225 | |
| [streptococcus constellatusas a causative agent of empyema. report of one case]. | streptococcus constellatus is a commensal microorganism in man but may cause infections in different locations. we report a 59 years old male with severe sequelae of a previous cerebrovascular accident that consulted in the emergency room for fever of 15 days of evolution. a right empyema was diagnosed. the bacteriological culture of the effusion disclosed the presence of streptococcus constellatus and two anaerobic strains (prevotella intermedia and fusobacterium urealyticus). the patient was t ... | 2006 | 17130992 |