Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
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| optimized use of the maldi biotyper system and the filmarray bcid panel for direct identification of microbial pathogens from positive blood cultures. | despite the current reliance on blood cultures (bcs), the diagnosis of bloodstream infections (bsis) can be sped up using new technologies performed directly on positive bc bottles. two methods (the maldi biotyper system and filmarray blood culture identification [bcid] panel) are potentially applicable. in this study, we performed a large-scale clinical evaluation (1,585 microorganisms from 1,394 bsi episodes) on the combined use of the maldi biotyper and filmarray bcid panel compared to a refe ... | 2016 | 26677254 |
| cheese microbial risk assessments - a review. | cheese is generally considered a safe and nutritious food, but foodborne illnesses linked to cheese consumption have occurred in many countries. several microbial risk assessments related to listeria monocytogenes, staphylococcus aureus, and escherichia coli infections, causing cheese-related foodborne illnesses, have been conducted. although the assessments of microbial risk in soft and low moisture cheeses such as semi-hard and hard cheeses have been accomplished, it has been more focused on t ... | 2016 | 26950859 |
| program and abstracts from the canadian digestive diseases week™ 2016. | 2016 | 27563641 | |
| maternal weaning modulates emotional behavior and regulates the gut-brain axis. | evidence shows that nutritional and environmental stress stimuli during postnatal period influence brain development and interactions between gut and brain. in this study we show that in rats, prevention of weaning from maternal milk results in depressive-like behavior, which is accompanied by changes in the gut bacteria and host metabolism. depressive-like behavior was studied using the forced-swim test on postnatal day (pnd) 25 in rats either weaned on pnd 21, or left with their mother until p ... | 2016 | 26903212 |
| reduced soluble rage is associated with disease severity of axonal guillain-barré syndrome. | soluble receptor for advanced glycation end products (srage) is an anti-inflammatory factor that mitigates the proinflammatory effects of high mobility group box 1 (hmgb1). the aim of this study was to investigate whether guillain-barré syndrome (gbs)-related inflammation are mediated by srage and hmgb1. we measured serum srage, hmgb1, il-6, and tnf-α levels in 86 patients with gbs and analysed associations between srage or hmgb1 and clinical variables in these subjects. in addition, we determin ... | 2016 | 26902096 |
| structural modeling of the flagellum ms ring protein flif reveals similarities to the type iii secretion system and sporulation complex. | the flagellum is a large proteinaceous organelle found at the surface of many bacteria, whose primary role is to allow motility through the rotation of a long extracellular filament. it is an essential virulence factor in many pathogenic species, and is also a priming component in the formation of antibiotic-resistant biofilms. the flagellum consists of the export apparatus on the cytosolic side; the basal body and rotor, spanning the bacterial membrane(s) and periplasm; and the hook-filament, t ... | 2016 | 26925337 |
| inflammation in irritable bowel syndrome: myth or new treatment target? | low-grade intestinal inflammation plays a key role in the pathophysiology of irritable bowel syndrome (ibs), and this role is likely to be multifactorial. the aim of this review was to summarize the evidence on the spectrum of mucosal inflammation in ibs, highlighting the relationship of this inflammation to the pathophysiology of ibs and its connection to clinical practice. we carried out a bibliographic search in medline and the cochrane library for the period of january 1966 to december 2014, ... | 2016 | 26900287 |
| cell wall anchoring of the campylobacter antigens to lactococcus lactis. | campylobacter jejuni is the most frequent cause of human food-borne gastroenteritis and chicken meat is the main source of infection. recent studies showed that broiler chicken immunization against campylobacter should be the most efficient way to lower the number of human infections by this pathogen. induction of the mucosal immune system after oral antigen administration should provide protective immunity to chickens. in this work we tested the usefulness of lactococcus lactis, the most extens ... | 2016 | 26925040 |
| distribution and genetic profiles of campylobacter in commercial broiler production from breeder to slaughter in thailand. | poultry and poultry products are commonly considered as the major vehicle of campylobacter infection in humans worldwide. to reduce the number of human cases, the epidemiology of campylobacter in poultry must be better understood. therefore, the objective of the present study was to determine the distribution and genetic relatedness of campylobacter in the thai chicken production industry. during june to october 2012, entire broiler production processes (i.e., breeder flock, hatchery, broiler fa ... | 2016 | 26886590 |
| spotyping: fast and accurate in silico mycobacterium spoligotyping from sequence reads. | spotyping is a fast and accurate program for in silico spoligotyping of mycobacterium tuberculosis isolates from next-generation sequencing reads. this novel method achieves high accuracy for reads of both uniform and varying lengths, and is about 20 to 40 times faster than spolpred. spotyping also integrates the function of producing a report summarizing associated epidemiological data from a global database of all isolates having the same spoligotype. spotyping is freely available at: https:// ... | 2016 | 26883915 |
| bacterial multidrug efflux pumps: much more than antibiotic resistance determinants. | bacterial multidrug efflux pumps are antibiotic resistance determinants present in all microorganisms. with few exceptions, they are chromosomally encoded and present a conserved organization both at the genetic and at the protein levels. in addition, most, if not all, strains of a given bacterial species present the same chromosomally-encoded efflux pumps. altogether this indicates that multidrug efflux pumps are ancient elements encoded in bacterial genomes long before the recent use of antibi ... | 2016 | 27681908 |
| utilization of carrageenan, citric acid and cinnamon oil as an edible coating of chicken fillets to prolong its shelf life under refrigeration conditions. | the present study was conducted to determine efficacy of edible coating of carrageenan and cinnamon oil to enhance the shelf life of chicken meat stored under refrigeration conditions. | 2016 | 27051203 |
| occurrence of campylobacter spp. in poultry meat at retail and processing plants' levels in central italy. | human campylobacteriosis remains the most commonly reported gastrointestinal disease in europe and campylobacter (c.) jejuni and c. coli are the two species most frequently involved in such foodborne disease. based on the sampling plan established in the region of lazio (central italy) the aim of our work was to investigate the occurrence of campylobacter spp. in poultry meat preparations collected by the local veterinary authority at retail shops and processing plants. we also observed whether ... | 2016 | 27800429 |
| development of a specific monoclonal antibody for the quantification of artemisinin in artemisia annua and rat serum. | artemisinin, extracted from artemisia annua, and its derivatives are important frontline antimalarials. to produce specific antibodies for the detection and quantification of artemisinin, artemisinin was transformed to 9-hydroxyartemisinin by microbial fermentation, which was used to prepare a 9-succinate artemisinin hapten for conjugation with ovalbumin. a monoclonal antibody (mab), designated as 3h7a10, was selected from hybridoma cell lines which showed high specificity to artemisinin. no com ... | 2016 | 26822789 |
| the epigenomic landscape of prokaryotes. | dna methylation acts in concert with restriction enzymes to protect the integrity of prokaryotic genomes. studies in a limited number of organisms suggest that methylation also contributes to prokaryotic genome regulation, but the prevalence and properties of such non-restriction-associated methylation systems remain poorly understood. here, we used single molecule, real-time sequencing to map dna modifications including m6a, m4c, and m5c across the genomes of 230 diverse bacterial and archaeal ... | 2016 | 26870957 |
| human campylobacteriosis in luxembourg, 2010-2013: a case-control study combined with multilocus sequence typing for source attribution and risk factor analysis. | campylobacteriosis has increased markedly in luxembourg during recent years. we sought to determine which campylobacter genotypes infect humans, where they may originate from, and how they may infect humans. multilocus sequence typing was performed on 1153 campylobacter jejuni and 136 c. coli human strains to be attributed to three putative animal reservoirs (poultry, ruminants, pigs) and to environmental water using the asymmetric island model. a nationwide case-control study (2010-2013) for do ... | 2016 | 26860258 |
| zika: the origin and spread of a mosquito-borne virus. | to describe the temporal and geographical distribution of zika virus infection and associated neurological disorders, from 1947 to 1 february 2016, when zika became a public health emergency of international concern (pheic). | 2016 | 27708473 |
| molecular mechanisms and potential clinical applications of campylobacter jejuni cytolethal distending toxin. | cytolethal distending toxin (cdt), a genotoxin produced by campylobacter jejuni, is composed of three subunits: cdta, cdtb, and cdtc. cdtb is a dnase that causes dna double-strand breaks (dsb) in the nucleus resulting in cell cycle arrest at the g2/m stage and apoptosis. cdta and cdtc bind to cholesterol-rich microdomains on the cytoplasmic membrane, a process required for the delivery of cdtb to cells. although a unique motif associated with cholesterol-binding activity has been identified in o ... | 2016 | 26904508 |
| tuning of hemes b equilibrium redox potential is not required for cross-membrane electron transfer. | in biological energy conversion, cross-membrane electron transfer often involves an assembly of two hemesb the hemes display a large difference in redox midpoint potentials (δem_b), which in several proteins is assumed to facilitate cross-membrane electron transfer and overcome a barrier of membrane potential. here we challenge this assumption reporting on hemebligand mutants of cytochromebc1in which, for the first time in transmembrane cytochrome, one natural histidine has been replaced by lysi ... | 2016 | 26858251 |
| genomic features of environmental and clinical vibrio parahaemolyticus isolates lacking recognized virulence factors are dissimilar. | vibrio parahaemolyticus is a bacterial pathogen that can cause illness after the consumption or handling of contaminated seafood. the primary virulence factors associated with v. parahaemolyticus illness are thermostable direct hemolysin (tdh) and tdh-related hemolysin (trh). however, clinical strains lacking tdh and trh have recently been isolated, and these clinical isolates are poorly understood. to help understand the emergence of clinical tdh- and trh-negative isolates, a genomic approach w ... | 2016 | 26637607 |
| immunomodulatory and anti-inflammatory activities of chicken cathelicidin-2 derived peptides. | host defence peptides and derived peptides are promising classes of antimicrobial and immunomodulatory lead compounds. for this purpose we examined whether chicken cathelicidin-2 (cath-2)-derived peptides modulate the function and inflammatory response of avian immune cells. using a chicken macrophage cell line (hd11) we found that full-length cath-2 dose-dependently induced transcription of chemokines cxcli2/il-8, mcp-3 and ccli4/rantes, but not of pro-inflammatory cytokine il-1β. in addition, ... | 2016 | 26848845 |
| the two-component system cpxra negatively regulates the locus of enterocyte effacement of enterohemorrhagic escherichia coli involving σ(32) and lon protease. | enterohemorrhagic escherichia coli (ehec) is a significant cause of serious human gastrointestinal disease worldwide. ehec strains contain a pathogenicity island called the locus of enterocyte effacement (lee), which encodes virulence factors responsible for damaging the gut mucosa. the cpx envelope stress response of e. coli is controlled by a two-component system (tcs) consisting of a sensor histidine kinase (cpxa) and a cytoplasmic response regulator (cpxr). in this study, we investigated the ... | 2016 | 26904510 |
| evolutionary genomics suggests that chev is an additional adaptor for accommodating specific chemoreceptors within the chemotaxis signaling complex. | escherichia coli and salmonella enterica are models for many experiments in molecular biology including chemotaxis, and most of the results obtained with one organism have been generalized to another. while most components of the chemotaxis pathway are strongly conserved between the two species, salmonella genomes contain some chemoreceptors and an additional protein, chev, that are not found in e. coli. the role of chev was examined in distantly related species bacillus subtilis and helicobacte ... | 2016 | 26844549 |
| multilocus sequence typing and antimicrobial resistance of campylobacter jejuni isolated from dairy calves in austria. | human campylobacteriosis is primarily associated with poultry but also cattle. in this study, 55 campylobacter jejuni strains isolated from 382 dairy calves' feces were differentiated by multilocus sequence typing and tested for antimicrobial resistance. the most prevalent sequence type (st) was st883 (20.0%), followed by st48 (14.5%), and st50 (9.1%). in contrast to st48 and st50, st883 has rarely been described in cattle previously. furthermore, risk factor analysis was performed for the prese ... | 2016 | 26870027 |
| recent development of mass spectrometry and proteomics applications in identification and typing of bacteria. | identification and typing of bacteria occupy a large fraction of time and work in clinical microbiology laboratories. with the certification of some ms platforms in recent years, more applications and tests of ms-based diagnosis methods for bacteria identification and typing have been created, not only on well-accepted maldi-tof-ms-based fingerprint matches, but also on solving the insufficiencies of maldi-tof-ms-based platforms and advancing the technology to areas such as targeted ms identific ... | 2016 | 26751976 |
| to what extent is faims beneficial in the analysis of proteins? | high field asymmetric waveform ion mobility spectrometry (faims), also known as differential ion mobility spectrometry, is emerging as a tool for biomolecular analysis. in this article, the benefits and limitations of faims for protein analysis are discussed. the principles and mechanisms of faims separation of ions are described, and the differences between faims and conventional ion mobility spectrometry are detailed. protein analysis is considered from both the top-down (intact proteins) and ... | 2016 | 26843211 |
| helicobacter pylori colonization in nepal; assessment of prevalence and potential risk factors in a hospital-based patient cohort. | helicobacter pylori, a gram-negative bacterium, can cause gastritis, peptic and duodenal ulcers. it is considered an important public health problem for both developed and developing world. this bacterium is classified as the class 1 carcinogen because it can cause cancer. | 2016 | 26833400 |
| cysteine mutational studies provide insight into a thiol-based redox switch mechanism of metal and dna binding in fura from anabaena sp. pcc 7120. | the ferric uptake regulator (fur) is the main transcriptional regulator of genes involved in iron homeostasis in most prokaryotes. fura from anabaena sp. pcc 7120 contains five cysteine residues, four of them arranged in two redox-active cxxc motifs. the protein needs not only metal but also reducing conditions to remain fully active in vitro. through a mutational study of the cysteine residues present in fura, we have investigated their involvement in metal and dna binding. | 2016 | 26414804 |
| magnetic resonance imaging differential diagnosis of brainstem lesions in children. | differential diagnosis of brainstem lesions, either isolated or in association with cerebellar and supra-tentorial lesions, can be challenging. knowledge of the structural organization is crucial for the differential diagnosis and establishment of prognosis of pathologies with involvement of the brainstem. familiarity with the location of the lesions in the brainstem is essential, especially in the pediatric population. magnetic resonance imaging (mri) is the most sensitive and specific imaging ... | 2016 | 26834941 |
| individual restriction of fine specificity variability in anti-gm1 igg antibodies associated with guillain-barré syndrome. | elevated titers of serum antibodies against gm1 ganglioside are associated with a variety of autoimmune neuropathies. much evidence indicates these autoantibodies play a primary role in the disease processes, but the mechanism for their appearance is unclear. we studied the fine specificity of anti-gm1 antibodies of the igg isotype present in sera from patients with guillain-barré syndrome (gbs), using thin-layer chromatogram-immunostaining of gm1, asialo-gm1 (ga1), gd1b and gm1-derivatives with ... | 2016 | 26818965 |
| diagnosing gastrointestinal illnesses using fecal headspace volatile organic compounds. | volatile organic compounds (vocs) emitted from stool are the components of the smell of stool representing the end products of microbial activity and metabolism that can be used to diagnose disease. despite the abundance of hydrogen, carbon dioxide, and methane that have already been identified in human flatus, the small portion of trace gases making up the vocs emitted from stool include organic acids, alcohols, esters, heterocyclic compounds, aldehydes, ketones, and alkanes, among others. thes ... | 2016 | 26819529 |
| an examination of co-infection in acute gastroenteritis and histo-blood group antigens leading to viral infection susceptibility. | the aim of the present study was to evaluate co-infection in the gastrointestinal tract in terms of viruses, bacteria and the abo blood group. we hypothesized that a combination of norovirus (nv) and bacteria in the gastrointestinal tract could affect the likelihood of an individual to contracting nv. histo-blood group antigens (hbgas) are considered to act as receptors that can lead to nv susceptibility. in addition to genetics, co-infection in the gastrointestinal tract may be associated with ... | 2016 | 26998270 |
| differential gene expression patterns in chicken cardiomyocytes during hydrogen peroxide-induced apoptosis. | hydrogen peroxide (h2o2) is both an exogenous and endogenous cytotoxic agent that can reliably induce apoptosis in numerous cell types for studies on apoptosis signaling pathways. however, little is known of these apoptotic processes in myocardial cells of chicken, a species prone to progressive heart failure. sequencing of mrna transcripts (rna-seq) allows for the identification of differentially expressed genes under various physiological and pathological conditions to elucidate the molecular ... | 2016 | 26808997 |
| novel α-l-fucosidases from a soil metagenome for production of fucosylated human milk oligosaccharides. | this paper describes the discovery of novel α-l-fucosidases and evaluation of their potential to catalyse the transglycosylation reaction leading to production of fucosylated human milk oligosaccharides. seven novel α-l-fucosidase-encoding genes were identified by functional screening of a soil-derived metagenome library and expressed in e. coli as recombinant 6xhis-tagged proteins. all seven fucosidases belong to glycosyl hydrolase family 29 (gh 29). six of the seven α-l-fucosidases were substr ... | 2016 | 26800369 |
| hib vaccines: past, present, and future perspectives. | haemophilus influenzae type b (hib) causes many severe diseases, including epiglottitis, pneumonia, sepsis, and meningitis. in developed countries, the annual incidence of meningitis caused by bacteria is approximately 5-10 cases per population of 100,000. the hib conjugate vaccine is considered protective and safe. adjuvants, molecules that can enhance and/or regulate the fundamental immunogenicity of an antigen, comprise a wide range of diverse compounds. while earlier developments of adjuvant ... | 2016 | 26904695 |
| evolutionary and functional relationships in the truncated hemoglobin family. | predicting function from sequence is an important goal in current biological research, and although, broad functional assignment is possible when a protein is assigned to a family, predicting functional specificity with accuracy is not straightforward. if function is provided by key structural properties and the relevant properties can be computed using the sequence as the starting point, it should in principle be possible to predict function in detail. the truncated hemoglobin family presents a ... | 2016 | 26788940 |
| within-host evolution of bacterial pathogens. | whole-genome sequencing has opened the way for investigating the dynamics and genomic evolution of bacterial pathogens during the colonization and infection of humans. the application of this technology to the longitudinal study of adaptation in an infected host--in particular, the evolution of drug resistance and host adaptation in patients who are chronically infected with opportunistic pathogens--has revealed remarkable patterns of convergent evolution, suggestive of an inherent repeatability ... | 2016 | 26806595 |
| molecular characterization and subtyping of blastocystis species in irritable bowel syndrome patients from north india. | blastocystis species has been extensively studied in recent few years to establish its pathogenecity. present study was designed to identify and examine the association of blastocystis sp. and its subtypes with irritable bowel syndrome (ibs).blastocystis sp. detected using wet-mount microscopy, trichrome staining, in-vitro culture and polymerase chain reaction (pcr) assay in a cohort of ibs patients (n = 150) and healthy controls (n = 100). isolates of blastocystis sp.were subtyped using sequenc ... | 2016 | 26784888 |
| phase variation of a type iig restriction-modification enzyme alters site-specific methylation patterns and gene expression in campylobacter jejuni strain nctc11168. | phase-variable restriction-modification systems are a feature of a diverse range of bacterial species. stochastic, reversible switches in expression of the methyltransferase produces variation in methylation of specific sequences. phase-variable methylation by both type i and type iii methyltransferases is associated with altered gene expression and phenotypic variation. one phase-variable gene of campylobacter jejuni encodes a homologue of an unusual type iig restriction-modification system in ... | 2016 | 26786317 |
| the influence of efflux pump inhibitors on the activity of non-antibiotic nsaids against gram-negative rods. | most patients with bacterial infections suffer from fever and various pains that require complex treatments with antibiotics, antipyretics, and analgaesics. the most common drugs used to relieve these symptoms are non-steroidal anti-inflammatory drugs (nsaids), which are not typically considered antibiotics. here, we investigate the effects of nsaids on bacterial susceptibility to antibiotics and the modulation of bacterial efflux pumps. | 2016 | 26771525 |
| alterations of the volatile metabolome in mouse models of alzheimer's disease. | in the present study, we tested whether the volatile metabolome was altered by mutations of the alzheimer's disease (ad)-implicated amyloid precursor protein gene (app) and comprehensively examined urinary volatiles that may potentially serve as candidate biomarkers of ad. establishing additional biomarkers in screening populations for ad will provide enhanced diagnostic specificity and will be critical in evaluating disease-modifying therapies. having strong evidence of gross changes in the vol ... | 2016 | 26762470 |
| association of ulcerative colitis with fut2 and fut3 polymorphisms in patients from southeast china. | dysbiosis of intestinal microbiota has been implicated in ulcerative colitis (uc). fucosyltransferase (fut) 2 and fut3 determine expression of histo-blood group antigens in the gut and may affect the intestinal microbiota. we investigated the association between fut2 and fut3 polymorphisms and uc in chinese patients. | 2016 | 26766790 |
| resistant starch alters the microbiota-gut brain axis: implications for dietary modulation of behavior. | the increasing recognition that the gut microbiota plays a central role in behavior and cognition suggests that the manipulation of microbial taxa through diet may provide a means by which behavior may be altered in a reproducible and consistent manner in order to achieve a beneficial outcome for the host. resistant starch continues to receive attention as a dietary intervention that can benefit the host through mechanisms that include altering the intestinal microbiota. given the interest in di ... | 2016 | 26745269 |
| rehabilitation of a patient with overlap of acute transverse myelitis and bickerstaff's brainstem encephalitis: a case report. | we report on one patient with bickerstaff's brainstem encephalitis (bbe) and associated flaccid weakness. counter to previous studies with bbe which indicate weakness due to guillain-barre syndrome, our patient's presentation of paraplegia following bbe is consistent with concomitant acute transverse myelitis. her findings of bbe largely resolved, although she remained with t6 american spinal injury association (asia) a paraplegia. motor functional impairment measure scores improved from 20 at a ... | 2016 | 28053734 |
| human milk components modulate toll-like receptor-mediated inflammation. | toll-like receptor (tlr) signaling is central to innate immunity. aberrant expression of tlrs is found in neonatal inflammatory diseases. several bioactive components of human milk modulate tlr expression and signaling pathways, including soluble toll-like receptors (stlrs), soluble cluster of differentiation (scd) 14, glycoproteins, small peptides, and oligosaccharides. some milk components, such as sialyl (α2,3) lactose and lacto-n-fucopentaose iii, are reported to increase tlr signaling; unde ... | 2016 | 26773018 |
| whole-genome sequencing analysis accurately predicts antimicrobial resistance phenotypes in campylobacter spp. | the objectives of this study were to identify antimicrobial resistance genotypes for campylobacter and to evaluate the correlation between resistance phenotypes and genotypes using in vitro antimicrobial susceptibility testing and whole-genome sequencing (wgs). a total of 114 campylobacter species isolates (82 c. coli and 32 c. jejuni) obtained from 2000 to 2013 from humans, retail meats, and cecal samples from food production animals in the united states as part of the national antimicrobial re ... | 2016 | 26519386 |
| removal of fecal indicators, pathogenic bacteria, adenovirus, cryptosporidium and giardia (oo)cysts in waste stabilization ponds in northern and eastern australia. | maturation ponds are used in rural and regional areas in australia to remove the microbial loads of sewage wastewater, however, they have not been studied intensively until present. using a combination of culture-based methods and quantitative real-time pcr, we assessed microbial removal rates in maturation ponds at four waste stabilization ponds (wsp) with (n = 1) and without (n = 3) baffles in rural and remote communities in australia. concentrations of total coliforms, e. coli, enterococci, c ... | 2016 | 26729150 |
| acute motor axonal neuropathy (aman) with motor conduction blocks in childhood; case report. | objective acute motor axonal neuropathy (aman), characterized with decreased compound muscle action potentials (cmap) and absence of demyelinating findings in electrophysiological studies, is a subtype of guillain-barre syndrome (gbs). a 4 yr-old male patient presented with ascending weakness, dysarthria and dysphagia to istanbul dr. lütfi kırdar kartal training and research hospital neurology outpatient for three days to in 2012. dysphonia, restricted eye movements, flaccid tetraplegia and aref ... | 2016 | 27057191 |
| multidrug resistant shigella flexneri infection simulating intestinal intussusception. | shigella enteritis remains an important cause of mortality and morbidity in all age groups, in developing as well as developed countries. owing to the emerging resistance to multiple antibiotics among shigella spp., it has been recognized as a major global public health concern and warrants constant monitoring of its resistance pattern. we report a case of segmental ileitis caused by non.-esbl producing multidrug resistant shigella flexneri in an infant clinically mimicking intussusception, whic ... | 2016 | 27013815 |
| sialic acids and autoimmune disease. | an important underlying mechanism that contributes to autoimmunity is the loss of inhibitory signaling in the immune system. sialic acid-recognizing ig superfamily lectins or siglecs are a family of cell surface proteins largely expressed in hematopoietic cells. the majority of siglecs are inhibitory receptors expressed in immune cells that bind to sialic acid-containing ligands and recruit sh2-domain-containing tyrosine phosphatases to their cytoplasmic tails. they deliver inhibitory signals th ... | 2016 | 26683151 |
| onset of ulcerative colitis during a low-carbohydrate weight-loss diet and treatment with a plant-based diet: a case report. | overweight and obesity are global health concerns. various effective weight-loss diets have been developed, including the atkins diet. the atkins diet is known as an extreme low-carbohydrate diet. this diet reduces body weight and has gained widespread popularity. however, the metabolite profiles of such a diet have been shown to be detrimental to colonic health. therefore, a concern for the long-term health effects of this diet exists. we encountered a case in which ulcerative colitis developed ... | 2016 | 26824967 |
| a comparison between plasmapheresis and intravenous immunoglobulin in children with guillain-barré syndrome in upper egypt. | the aim of our study is to assess the clinico-electrophysiological profile of children with guillain-barré syndrome (gbs) in upper egypt and to compare the efficacy of plasmapheresis versus other treatment modalities. | 2016 | 26788127 |
| a gastrointestinal anti-infectious biotherapeutic agent: the heat-treated lactobacillus lb. | experimental in vitro and in vivo studies support the hypothesis that heat-treated, lyophilized lactobacillus acidophilus lb cells and concentrated, neutralized spent culture medium conserve the variety of pharmacological, antimicrobial activities of the live probiotic strain against several infectious agents involved in well-established acute and persistent watery diarrhoea and gastritis. heat-treated cells and heat-stable secreted molecules trigger multiple strain-specific activities explainin ... | 2016 | 26770268 |
| prevalence and risk factors for campylobacter spp., salmonella spp., coxiella burnetii, and newcastle disease virus in feral pigeons (columba livia) in public areas of montreal, canada. | feral pigeons (columbia livia) can harbor a range of zoonotic pathogens. a transversal study was undertaken to estimate the prevalence of feral pigeons infected by various pathogens in public areas in montreal, quebec. cloacal swabs from captured birds were cultured for salmonella spp. and campylobacter spp. and tested by real-time polymerase chain reaction (rt-pcr) for the detection of coxiella burnetii. an oropharyngeal swab was also submitted to real-time reverse-transcription polymerase chai ... | 2016 | 26733736 |
| antimicrobial usage and resistance in beef production. | antimicrobials are critical to contemporary high-intensity beef production. many different antimicrobials are approved for beef cattle, and are used judiciously for animal welfare, and controversially, to promote growth and feed efficiency. antimicrobial administration provides a powerful selective pressure that acts on the microbial community, selecting for resistance gene determinants and antimicrobial-resistant bacteria resident in the bovine flora. the bovine microbiota includes many harmles ... | 2016 | 27999667 |
| long-term impact after fulminant guillain-barré syndrome, case report and literature review. | a 47-year-old man was admitted to the intensive care unit a few hours after presenting to emergency department with acute diplopia and dysphonia. swallowing disorders and respiratory muscular weakness quickly required invasive ventilation. on day 3, the patient was in a "brain-death"-like state with deep coma and absent brainstem reflexes. electroencephalogram ruled out brain death diagnosis as a paradoxical sleep trace was recorded. cerebrospinal fluid analysis, electrophysiologic studies, and ... | 2016 | 27853394 |
| an overview of the control of bacterial pathogens in cattle manure. | cattle manure harbors microbial constituents that make it a potential source of pollution in the environment and infections in humans. knowledge of, and microbial assessment of, manure is crucial in a bid to prevent public health and environmental hazards through the development of better management practices and policies that should govern manure handling. physical, chemical and biological methods to reduce pathogen population in manure do exist, but are faced with challenges such as cost, odor ... | 2016 | 27571092 |
| clinical and epidemiological analysis of campylobacter fetus subsp. fetus infections in humans and comparative genetic analysis with strains isolated from cattle. | campylobacter fetus subspecies fetus (cff) is an important pathogen for both cattle and humans. we performed a systematic epidemiological and clinical study of patients and evaluated the genetic relatedness of 17 human and 17 bovine cff isolates by using different genotyping methods. in addition, the serotype, the dissemination of the genomic island containing a type iv secretion system (t4ss) and resistance determinants for tetracycline and streptomycin were also evaluated. | 2016 | 27177684 |
| campylobacter jejuni fatal sepsis in a patient with non-hodgkin's lymphoma: case report and literature review of a difficult diagnosis. | campylobacter jejuni (c. jejuni) bacteremia is difficult to diagnose in individuals with hematological disorders undergoing chemotherapy. the cause can be attributed to the rarity of this infection, to the variable clinical presentation, and to the partial overlapping symptoms underlying the disease. here, we report a case of a fatal sepsis caused by c. jejuni in a 76-year-old caucasian man with non-hodgkin's lymphoma. after chemotherapeutic treatment, the patient experienced fever associated wi ... | 2016 | 27077849 |
| reptile toll-like receptor 5 unveils adaptive evolution of bacterial flagellin recognition. | toll-like receptors (tlr) are ancient innate immune receptors crucial for immune homeostasis and protection against infection. tlrs are present in mammals, birds, amphibians and fish but have not been functionally characterized in reptiles despite the central position of this animal class in vertebrate evolution. here we report the cloning, characterization, and function of tlr5 of the reptile anolis carolinensis (green anole lizard). the receptor (actlr5) displays the typical tlr protein archit ... | 2016 | 26738735 |
| bacteriophage-based tools: recent advances and novel applications. | bacteriophages (phages) are viruses that infect bacterial hosts, and since their discovery over a century ago they have been primarily exploited to control bacterial populations and to serve as tools in molecular biology. in this commentary, we highlight recent diverse advances in the field of phage research, going beyond bacterial control using whole phage, to areas including biocontrol using phage-derived enzybiotics, diagnostics, drug discovery, novel drug delivery systems and bionanotechnolo ... | 2016 | 27990274 |
| bibliometric analysis of publications on campylobacter: (2000-2015). | campylobacter species are widespread zoonotic pathogens. campylobacter jejuni causes a form of gastroenteritis called campylobacteriosis. campylobacter drug resistance is considered a serious threat. in order to better understand national and international research output on campylobacter, we conducted this bibliometric overview of publications on campylobacter. this study can be used to assess extent of interaction and response of researchers, food regulators, and health policy makers to global ... | 2016 | 27899145 |
| exploiting bacterial whole-genome sequencing data for evaluation of diagnostic assays: campylobacter species identification as a case study. | the application of whole-genome sequencing (wgs) to problems in clinical microbiology has had a major impact on the field. clinical laboratories are now using wgs for pathogen identification, antimicrobial susceptibility testing, and epidemiological typing. wgs data also represent a valuable resource for the development and evaluation of molecular diagnostic assays, which continue to play an important role in clinical microbiology. to demonstrate this application of wgs, this study used publicly ... | 2016 | 27733632 |
| glucose metabolism via the entner-doudoroff pathway in campylobacter: a rare trait that enhances survival and promotes biofilm formation in some isolates. | isolates of the zoonotic pathogen campylobacter are generally considered to be unable to metabolize glucose due to lack of key glycolytic enzymes. however, the entner-doudoroff (ed) pathway has been identified in campylobacter jejuni subsp. doylei and a few c. coli isolates. a systematic search for ed pathway genes in a wide range of campylobacter isolates and in the c. jejuni/coli pubmlst database revealed that 1.7% of >6,000 genomes encoded a complete ed pathway, including both c. jejuni and c ... | 2016 | 27920773 |
| advances and challenges in viability detection of foodborne pathogens. | foodborne outbreaks are a serious public health and food safety concern worldwide. there is a great demand for rapid, sensitive, specific, and accurate methods to detect microbial pathogens in foods. conventional methods based on cultivation of pathogens have been the gold standard protocols; however, they take up to a week to complete. molecular assays such as polymerase chain reaction (pcr), sequencing, microarray technologies have been widely used in detection of foodborne pathogens. among mo ... | 2016 | 27920757 |
| epidemiology and rapd-pcr typing of thermophilic campylobacters from children under five years and chickens in morogoro municipality, tanzania. | campylobacter species are gram negative and flagellated bacteria under the genus campylobacter, family campylobacteriaceae. these pathogens cause zoonotic infections among human and animal populations. this study was undertaken between december 2006 and may 2007 to determine prevalence, risk factors and genetic diversity of thermophilic campylobacter isolates from children less than 5 years and chickens in morogoro municipality, tanzania. | 2016 | 27871251 |
| prevalence of enteropathogens in dogs attending 3 regional dog parks in northern california. | the prevalence and risk factors for infection with enteropathogens in dogs frequenting dog parks have been poorly documented, and infected dogs can pose a potential zoonotic risk for owners. | 2016 | 27859745 |
| momp from campylobacter jejuni is a trimer of 18-stranded β-barrel monomers with a ca(2+) ion bound at the constriction zone. | the gram-negative organism campylobacter jejuni is the major cause of food poisoning. unlike escherichia coli, which has two major porins, ompc and ompf, c. jejuni has one, termed major outer membrane protein (momp) through which nutrients and antibiotics transit. we report the 2.1-å crystal structure of c. jejuni momp expressed in e. coli and a lower resolution but otherwise identical structure purified directly from c. jejuni. the 2.1-å resolution structure of recombinant momp showed that alth ... | 2016 | 27693650 |
| evaluation of a microfluidic chip system for preparation of bacterial dna from swabs, air, and surface water samples. | the detection of bacterial pathogens from complex sample matrices by pcr requires efficient dna extraction. in this study, a protocol for extraction and purification of dna from swabs, air, and water samples using a microfluidic chip system was established. the optimized protocol includes a combination of thermal, chemical and enzymatic lysis followed by chip-based dna purification using magnetic particles. the procedure was tested using gram-positive bacillus thuringiensis berliner var. kurstak ... | 2016 | 27520284 |
| development of a droplet digital polymerase chain reaction for rapid and simultaneous identification of common foodborne pathogens in soft cheese. | dairy products can harbor various microorganisms (e.g., campylobacter spp., salmonella spp., listeria monocytogenes, verocytotoxin-producing escherichia coli) arising from animal reservoirs, and which can become important sources of foodborne illness. therefore, early detection of food pathogens is crucial to prevent diseases. we wished to develop an accurate quantitative protocol based on a droplet digital polymerase chain reaction (ddpcr) involving eight individual taqman™ reactions to detect ... | 2016 | 27840628 |
| prevalence of campylobacter jejuni and campylobacter coli in raw milk and some dairy products. | this study was accomplished to test raw milk and certain dairy products sold in local markets of qena, egypt, for the presence of campylobacter coli and campylobacter jejuni. | 2016 | 27847427 |
| molecular epidemiology, virulence determinants and antimicrobial resistance of campylobacter spreading in retail chicken meat in central china. | campylobacter species are the major food-borne pathogens which could cause bacterial gastroenteritis in humans. contaminated chicken products have been recognized as the primary vehicles of campylobacter transmission to human beings. in this study, the prevalence of campylobacter in retail chicken meat in central china was investigated, and the isolates were further characterized using molecular approaches and tested for antibiotic resistance. | 2016 | 27800028 |
| a direct-sensing galactose chemoreceptor recently evolved in invasive strains of campylobacter jejuni. | a rare chemotaxis receptor, tlp11, has been previously identified in invasive strains of campylobacter jejuni, the most prevalent cause of bacterial gastroenteritis worldwide. here we use glycan and small-molecule arrays, as well as surface plasmon resonance, to show that tlp11 specifically interacts with galactose. tlp11 is required for the chemotactic response of c. jejuni to galactose, as shown using wild type, allelic inactivation and addition mutants. the inactivated mutant displays reduced ... | 2016 | 27762269 |
| complete genome sequences of multidrug-resistant campylobacter jejuni strain 14980a (turkey feces) and campylobacter coli strain 14983a (housefly from a turkey farm), harboring a novel gentamicin resistance mobile element. | multidrug resistance (mdr) in foodborne pathogens is a major food safety and public health issue. here we describe whole-genome sequences of two mdr strains of campylobacter jejuni and campylobacter coli from turkey feces and a housefly from a turkey farm. both strains harbor a novel chromosomal gentamicin resistance mobile element. | 2016 | 27795285 |
| the risk of some veterinary antimicrobial agents on public health associated with antimicrobial resistance and their molecular basis. | the risk of antimicrobial agents used in food-producing animals on public health associated with antimicrobial resistance continues to be a current topic of discussion as related to animal and human public health. in the present review, resistance monitoring data, and risk assessment results of some important antimicrobial agents were cited to elucidate the possible association of antimicrobial use in food animals and antimicrobial resistance in humans. from the selected examples, it was apparen ... | 2016 | 27803693 |
| new weapons to fight old enemies: novel strategies for the (bio)control of bacterial biofilms in the food industry. | biofilms are microbial communities characterized by their adhesion to solid surfaces and the production of a matrix of exopolymeric substances, consisting of polysaccharides, proteins, dna and lipids, which surround the microorganisms lending structural integrity and a unique biochemical profile to the biofilm. biofilm formation enhances the ability of the producer/s to persist in a given environment. pathogenic and spoilage bacterial species capable of forming biofilms are a significant problem ... | 2016 | 27803696 |
| use of direct lamp screening of broiler fecal samples for campylobacter jejuni and campylobacter coli in the positive flock identification strategy. | rapid identification of campylobacter-positive flocks before slaughter, following freezing and heat treatment for the campylobacter-positive carcasses at the slaughterhouses is an effective control strategy against foodborne campylobacteriosis. we evaluated a loop-mediated isothermal amplification (lamp) assay for the direct screening of naturally contaminated chicken cloacal swabs for c. jejuni/c. coli to compare this assay with conventional quantitative culture methods. in a comparison study o ... | 2016 | 27746777 |
| extraction of total nucleic acids from bacterial isolates using the biomérieux nuclisens easymag total nucleic acid extractor. | the biomerieux nuclisens easymag total nucleic acid extractor was evaluated for use on bacterial isolates in the clinical microbiology laboratory. forty eight isolates were extracted, yielding quantifiable amounts of dna for all isolates. the easymag is appropriate for dna extraction from bacterial isolates and will be incorporated in the clinical laboratory. | 2016 | 27658494 |
| ethyl pyruvate: an anti-microbial agent that selectively targets pathobionts and biofilms. | the microbiota has a strong influence on health and disease in humans. a causative shift favoring pathobionts is strongly linked to diseases. therefore, anti-microbial agents selectively targeting potential pathogens as well as their biofilms are urgently demanded. here we demonstrate the impact of ethyl pyruvate, so far known as ros scavenger and anti-inflammatory agent, on planktonic microbes and biofilms. ethyl pyruvate combats preferably the growth of pathobionts belonging to bacteria and fu ... | 2016 | 27658257 |
| emergence of a potent multidrug efflux pump variant that enhances campylobacter resistance to multiple antibiotics. | bacterial antibiotic efflux pumps are key players in antibiotic resistance. although their role in conferring multidrug resistance is well documented, the emergence of "super" efflux pump variants that enhance bacterial resistance to multiple drugs has not been reported. here, we describe the emergence of a resistance-enhancing variant (named re-cmeabc) of the predominant efflux pump cmeabc in campylobacter, a major zoonotic pathogen whose resistance to antibiotics is considered a serious antibi ... | 2016 | 27651364 |
| prevalence and antibiotic resistance profile of thermophilic campylobacter spp. of slaughtered cattle and sheep in shiraz, iran | although poultry meat is considered as the main source for human campylobacter infections, there is limited information about non-poultry sources. the present study was aimed to investigate the prevalence and the antibiotic resistance of thermophilic campylobacter spp. in fecal samples of the cattle and sheep in shiraz, iran. a total of 302 fecal samples were obtained from clinically healthy, slaughtered cattle and sheep from shiraz slaughterhouse. the animals were clinically healthy before bein ... | 2016 | 27872721 |
| quantification of growth of campylobacter and extended spectrum β-lactamase producing bacteria sheds light on black box of enrichment procedures. | campylobacter is well recognized as the leading cause of bacterial foodborne diarrheal disease worldwide, and is routinely found in meat originating from poultry, sheep, pigs, and cattle. effective monitoring of campylobacter contamination is dependent on the availability of reliable detection methods. the method of the international organization for standardization for the detection of campylobacter spp. in food (iso 10272-1:2006) recommends the use of bolton broth (bb) as selective enrichment ... | 2016 | 27672384 |
| polyphosphate and associated enzymes as global regulators of stress response and virulence in campylobacter jejuni. | campylobacter jejuni (c. jejuni), a gram-negative microaerophilic bacterium, is a predominant cause of bacterial foodborne gastroenteritis in humans worldwide. despite its importance as a major foodborne pathogen, our understanding of the molecular mechanisms underlying c. jejuni stress survival and pathogenesis is limited. inorganic polyphosphate (poly p) has been shown to play significant roles in bacterial resistance to stress and virulence in many pathogenic bacteria. c. jejuni contains the ... | 2016 | 27672264 |
| point mutations in the major outer membrane protein drive hypervirulence of a rapidly expanding clone of campylobacter jejuni. | infections due to clonal expansion of highly virulent bacterial strains are clear and present threats to human and animal health. association of genetic changes with disease is now a routine, but identification of causative mutations that enable disease remains difficult. campylobacter jejuni is an important zoonotic pathogen transmitted to humans mainly via the foodborne route. c. jejuni typically colonizes the gut, but a hypervirulent and rapidly expanding clone of c. jejuni recently emerged, ... | 2016 | 27601641 |
| a new lab developed real time pcr assay for direct detection of c. difficle from stool sample without dna extraction. | clostridium difficile is a major cause of nosocomial antibiotic-associated infectious diarrhea and pseudomembranous colitis. detection of c. difficile by anaerobic bacterial culture and/or cytotoxicity assays has been largely replaced by rapid enzyme immunoassays (eia). however, due to the lack of sensitivity of stool eia, we developed a multiplex real-time pcr assay targeting the c. difficile toxin genes tcdb. stool samples from hospitalized pediatric patients suspected of having c. difficile-a ... | 2016 | 27829823 |
| antimicrobial resistance of campylobacter isolates from small scale and backyard chicken in kenya. | thermophilic campylobacter species are a major cause of bacterial foodborne diarrhoea in humans worldwide. poultry and their products are the predominant source for human campylobacteriosis. resistance of campylobacter to antibiotics is increasing worldwide, but little is known about the antibiotic resistance in campylobacter isolated from chicken in kenya. in this study, 35 suspected campylobacter strains isolated from faeces and cloacal swabs of chicken were tested for their susceptibility to ... | 2016 | 27570543 |
| whole-genome sequences of agricultural, host-associated campylobacter coli and campylobacter jejuni strains. | we report here the genome sequences of four agricultural, multidrug-resistant campylobacter spp.: c. coli 11601 and c. jejuni 11601md, isolated from turkey cecum and jejunum, respectively, and c. coli 6067 and c. coli 6461, isolated from turkey-house water and swine feces, respectively. the genomes provide insights on campylobacter antimicrobial resistance and host adaptations. | 2016 | 27540063 |
| mosaic tetracycline resistance genes encoding ribosomal protection proteins. | first reported in 2003, mosaic tetracycline resistance genes are a subgroup of the genes encoding ribosomal protection proteins (rpps). they are formed when two or more rpp-encoding genes recombine resulting in a functional chimera. to date, the majority of mosaic genes are derived from sections of three rpp genes, tet(o), tet(w) and tet(32), with others comprising tet(m) and tet(s). in this first review of mosaic genes, we report on their structure, diversity and prevalence, and suggest that th ... | 2016 | 27494928 |
| association of campylobacter jejuni ssp. jejuni chemotaxis receptor genes with multilocus sequence types and source of isolation. | campylobacter jejuni's flagellar locomotion is controlled by eleven chemoreceptors. assessment of the distribution of the relevant chemoreceptor genes in the c. jejuni genomes deposited in the national center for biotechnology information (ncbi) database led to the identification of two previously unknown tlp genes and a tlp5 pseudogene. these two chemoreceptor genes share the same locus in the c. jejuni genome with tlp4 and tlp11, but the gene region encoding the periplasmic ligand binding doma ... | 2016 | 27766165 |
| evaluation of various campylobacter-specific quantitative pcr (qpcr) assays for detection and enumeration of campylobacteraceae in irrigation water and wastewater via a miniaturized most-probable-number-qpcr assay. | campylobacter spp. are the leading cause of bacterial gastroenteritis worldwide, and water is increasingly seen as a risk factor in transmission. here we describe a most-probable-number (mpn)-quantitative pcr (qpcr) assay in which water samples are centrifuged and aliquoted into microtiter plates and the bacteria are enumerated by qpcr. we observed that commonly used campylobacter molecular assays produced vastly different detection rates. in irrigation water samples, detection rates varied depe ... | 2016 | 27235434 |
| subtyping of salmonella enterica subspecies i using single-nucleotide polymorphisms in adenylate cyclase. | methods to rapidly identify serotypes of salmonella enterica subspecies i are of vital importance for protecting the safety of food. to supplement the serotyping method dkgb-linked intergenic sequence ribotyping (isr), single-nucleotide polymorphisms were characterized within adenylate cyclase (cyaa). the national center for biotechnology information (ncbi) database had 378 cyaa sequences from s. enterica subspecies i, which included 42 unique dna sequences and 19 different amino acid sequences. ... | 2016 | 27035032 |
| campylobacter species and neutrophilic inflammatory bowel disease in cats. | inflammatory bowel disease (ibd) is a common cause of signs of gastrointestinal disease in cats. a subset of cats with ibd has neutrophilic inflammation of the intestinal mucosa. | 2016 | 27362482 |
| evidence of avian and possum fecal contamination in rainwater tanks as determined by microbial source tracking approaches. | avian and possum fecal droppings may negatively impact roof-harvested rainwater (rhrw) water quality due to the presence of zoonotic pathogens. this study was aimed at evaluating the performance characteristics of a possum feces-associated (psm) marker by screening 210 fecal and wastewater samples from possums (n = 20) and a range of nonpossum hosts (n = 190) in southeast queensland, australia. the host sensitivity and specificity of the psm marker were 0.90 and 0.95 (maximum value, 1.00), respe ... | 2016 | 27208100 |
| molecular epidemiology of campylobacter coli strains isolated from different sources in new zealand between 2005 and 2014. | campylobacteriosis is one of the most important foodborne diseases worldwide and a significant health burden in new zealand. campylobacter jejuni is the predominant species worldwide, accounting for approximately 90% of human cases, followed by campylobacter coli most studies in new zealand have focused on c. jejuni; hence, the impact of c. coli strains on human health is not well understood. the aim of this study was to genotype c. coli isolates collected in the manawatu region of new zealand f ... | 2016 | 27208097 |
| optimization of quantitative pcr methods for enteropathogen detection. | detection and quantification of enteropathogens in stool specimens is useful for diagnosing the cause of diarrhea but is technically challenging. here we evaluate several important determinants of quantification: specimen collection, nucleic acid extraction, and extraction and amplification efficiency. first, we evaluate the molecular detection and quantification of pathogens in rectal swabs versus stool, using paired flocked rectal swabs and whole stool collected from 129 children hospitalized ... | 2016 | 27336160 |
| potential use of fosfomycin-tromethamine for treatment of recurrent campylobacter species enteritis. | we report 2 cases of recurrent campylobacter coli enteritis caused by macrolide- and fluoroquinolone-resistant strains in 2 patients with hypogammaglobulinemia, successfully treated with a prolonged course of fosfomycin-tromethamine with no side effects. fosfomycin-tromethamine may be a feasible alternative therapy for recurrent enteritis caused by campylobacter species resistant to first-line drugs. | 2016 | 27161640 |
| identification of novel vaccine candidates against campylobacter through reverse vaccinology. | campylobacteriosis is the most prevalent bacterial foodborne gastroenteritis affecting humans in the european union. human cases are mainly due to campylobacter jejuni or campylobacter coli, and contamination is associated with the handling and/or consumption of poultry meat. in fact, poultry constitutes the bacteria's main reservoir. a promising way of decreasing the incidence of campylobacteriosis in humans would be to decrease avian colonization. poultry vaccination is of potential for this p ... | 2016 | 27413761 |
| pfge genotyping and molecular characterization of campylobacter spp. isolated from chicken meat. | a total of 70 samples were collected from chicken meat obtained from 10 markets in tehran, iran from which 39 campylobacter coli were isolated. among 10 antibiotics used, maximum resistance was seen to trimethoprim-sulphamethoxazole (sxt) (97.36%), nalidixic acid (94.8%), ciprofloxacin (87.7%), streptomycin (89.72%), and tetracycline (97.4%). no resistance was to gentamycin was observed. none of the campylobacter strains under study harbored integron, suggesting the involvement of other resistan ... | 2016 | 27822247 |
| optimized cultivation of campylobacter concisus from gut mucosal biopsies in inflammatory bowel disease. | campylobacter concisus is a commensal of the human oral flora that has been linked to prolonged diarrhea and inflammatory bowel disease (ibd). it has been detected more often from intestinal biopsies in patients with ibd compared to healthy controls using pcr-based techniques, whereas the number of c. concisus culture-positive biopsies in previous studies has been very limited. determining the rate of viable isolates present in the gut mucosa is of great importance when evaluating the role in di ... | 2016 | 27252786 |
| whole-genome sequencing of a campylobacter jejuni strain isolated from retail chicken meat reveals the presence of a megaplasmid with mu-like prophage and multidrug resistance genes. | genome sequencing of campylobacter jejuni strain t1-21 isolated from retail chicken meat revealed the presence of a chromosome of 1,565,978 bp and a megaplasmid of 82,732 bp that contains mu-like prophage and multidrug resistance genes. this is the first reported sequence of a campylobacter megaplasmid >55 kb. | 2016 | 27231378 |