Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| identification of residues controlling restriction versus enhancing activities of ifitm proteins on the entry of human coronaviruses. | interferon-induced transmembrane proteins (ifitm) are restriction factors that inhibit the infectious entry of many enveloped rna viruses. however, we demonstrated previously that human ifitm2 and ifitm3 are essential host factors facilitating the entry of human coronavirus (hcov)-oc43. in a continuing effort to decipher the molecular mechanism underlying ifitm differential modulation of hcov entry, we investigated the role of structural motifs important for ifitm protein post-translational modi ... | 2017 | 29263263 |
| coronaviruses and arteriviruses display striking differences in their cyclophilin a-dependence during replication in cell culture. | cyclophilin a (cypa) is an important host factor in the replication of a variety of rna viruses. also the replication of several nidoviruses was reported to depend on cypa, although possibly not to the same extent. these prior studies are difficult to compare, since different nidoviruses, cell lines and experimental set-ups were used. here, we investigated the cypa dependence of three distantly related nidoviruses that can all replicate in huh7 cells: the arterivirus equine arteritis virus (eav) ... | 2017 | 29249267 |
| cross-sectional study of mers-cov-specific rna and antibodies in animals that have had contact with mers patients in saudi arabia. | middle east respiratory syndrome coronavirus (mers-cov) is a newly emerged coronavirus that is associated with a severe respiratory disease in humans in the middle east. the epidemiological profiles of the mers-cov infections suggest zoonotic transmission from an animal reservoir to humans. | 2017 | 28993171 |
| identification of sialic acid-binding function for the middle east respiratory syndrome coronavirus spike glycoprotein. | middle east respiratory syndrome coronavirus (mers-cov) targets the epithelial cells of the respiratory tract both in humans and in its natural host, the dromedary camel. virion attachment to host cells is mediated by 20-nm-long homotrimers of spike envelope protein s. the n-terminal subunit of each s protomer, called s1, folds into four distinct domains designated s1athrough s1dbinding of mers-cov to the cell surface entry receptor dipeptidyl peptidase 4 (dpp4) occurs via s1bwe now demonstrate ... | 2017 | 28923942 |
| identification of diverse viruses in upper respiratory samples in dromedary camels from united arab emirates. | camels are known carriers for many viral pathogens, including middle east respiratory syndrome coronavirus (mers-cov). it is likely that there are additional, as yet unidentified viruses in camels with the potential to cause disease in humans. in this study, we performed metagenomic sequencing analysis on nasopharyngeal swab samples from 108 mers-cov-positive dromedary camels from a live animal market in abu dhabi, united arab emirates. we obtained a total of 846.72 million high-quality reads fr ... | 2017 | 28902913 |
| a novel dynamic model describing the spread of the mers-cov and the expression of dipeptidyl peptidase 4. | the middle east respiratory syndrome (mers) coronavirus, a newly identified pathogen, causes severe pneumonia in humans. mers is caused by a coronavirus known as mers-cov, which attacks the respiratory system. the recently defined receptor for mers-cov, dipeptidyl peptidase 4 (dpp4), is generally expressed in endothelial and epithelial cells and has been shown to be present on cultured human nonciliated bronchiolar epithelium cells. in this paper, a class of novel four-dimensional dynamic model ... | 2017 | 28894474 |
| [construction and identification of nanobody phage display library targeting middle east respiratory syndrome coronavirus]. | objective to construct a phage display library of specific nano-antibodies against the middle east respiratory syndrome coronavirus (mers-cov) and apply it to the screening of neutralizing nano-antibodies. methods mers-cov receptor-binding domain (rbd) recombinant protein was used to immunize alpaca. after the last immunization, peripheral blood mononuclear cells (pbmcs) were isolated from the whole blood and total rna was extracted. the vhh gene was amplified by pcr and used to construct recomb ... | 2017 | 29382428 |
| transgene expression in the genome of middle east respiratory syndrome coronavirus based on a novel reverse genetics system utilizing red-mediated recombination cloning. | middle east respiratory syndrome coronavirus (mers-cov) is a high-priority pathogen in pandemic preparedness research. reverse genetics systems are a valuable tool to study viral replication and pathogenesis, design attenuated vaccines and create defined viral assay systems for applications such as antiviral screening. here we present a novel reverse genetics system for mers-cov that involves maintenance of the full-length viral genome as a cdna copy inserted in a bacterial artificial chromosome ... | 2017 | 28984231 |
| identification of alpha and beta coronavirus in wildlife species in france: bats, rodents, rabbits, and hedgehogs. | coronaviruses are closely monitored in the context of emerging diseases and, as illustrated with severe acute respiratory syndrome coronavirus (sars-cov) and middle east respiratory syndrome-coronavirus (mers-cov), are known to cross the species barrier and eventually to move from wildlife to humans. knowledge of the diversity of coronaviruses in wildlife is therefore essential to better understand and prevent emergence events. this study explored the presence of coronaviruses in four wild mamma ... | 2017 | 29186061 |
| expression and cleavage of middle east respiratory syndrome coronavirus nsp3-4 polyprotein induce the formation of double-membrane vesicles that mimic those associated with coronaviral rna replication. | betacoronaviruses, such as middle east respiratory syndrome coronavirus (mers-cov), are important pathogens causing potentially lethal infections in humans and animals. coronavirus rna synthesis is thought to be associated with replication organelles (ros) consisting of modified endoplasmic reticulum (er) membranes. these are transformed into double-membrane vesicles (dmvs) containing viral double-stranded rna and into other membranous elements such as convoluted membranes, together forming a re ... | 2017 | 29162711 |
| spectrum of pathogen- and model-specific histopathologies in mouse models of acute pneumonia. | pneumonia may be caused by a wide range of pathogens and is considered the most common infectious cause of death in humans. murine acute lung infection models mirror human pathologies in many aspects and contribute to our understanding of the disease and the development of novel treatment strategies. despite progress in other fields of tissue imaging, histopathology remains the most conclusive and practical read out tool for the descriptive and semiquantitative evaluation of mouse pneumonia and ... | 2017 | 29155867 |
| middle east respiratory syndrome coronavirus nonstructural protein 16 is necessary for interferon resistance and viral pathogenesis. | coronaviruses (covs) encode a mixture of highly conserved and novel genes, as well as genetic elements necessary for infection and pathogenesis, raising the possibility of common targets for attenuation and therapeutic design. in this study, we focused on highly conserved nonstructural protein 16 (nsp16), a viral 2'o-methyltransferase (2'o-mtase) that encodes critical functions in immune modulation and infection. using reverse genetics, we disrupted a key motif in the conserved kdke motif of mid ... | 2017 | 29152578 |
| diversity of middle east respiratory syndrome coronaviruses in 109 dromedary camels based on full-genome sequencing, abu dhabi, united arab emirates. | middle east respiratory syndrome coronavirus (mers-cov) was identified on the arabian peninsula in 2012 and is still causing cases and outbreaks in the middle east. when mers-cov was first identified, the closest related virus was in bats; however, it has since been recognized that dromedary camels serve as a virus reservoir and potential source for human infections. a total of 376 camels were screened for mers-cov at a live animal market in the eastern region of the emirate of abu dhabi, uae. i ... | 2017 | 29116217 |
| knowledge and awareness of middle east respiratory syndrome coronavirus among saudi and non-saudi arabian pilgrims. | the current study was intended to evaluate the knowledge and awareness toward middle east respiratory syndrome coronavirus (mers-cov) of pilgrims from saudi arabia and from different arabian countries. | 2017 | 29114190 |
| pathogen genomic surveillance elucidates the origins, transmission and evolution of emerging viral agents in china. | in the past twenty years, numerous novel zoonotic viral agents with pandemic potential have emerged in china, such as the severe acute respiratory syndrome (sars) coronavirus and, more recently, the avian-origin influenza a/h7n9 virus, which have caused outbreaks among humans with high morbidity and mortality. in addition, several emerging and re-emerging viral pathogens have also been imported into china from travelers, e.g. the middle east respiratory syndrome (mers) coronavirus and zika virus ... | 2017 | 29270793 |
| protective efficacy of a novel simian adenovirus vaccine against lethal mers-cov challenge in a transgenic human dpp4 mouse model. | middle east respiratory syndrome coronavirus (mers-cov) is a novel zoonotic virus that causes severe respiratory disease in humans with a case fatality rate close to 40%, but for which no vaccines are available. here, we evaluated the utility of chadox1, a promising replication-deficient simian adenovirus vaccine vector platform with an established safety profile in humans and dromedary camels, for mers-cov vaccine development. using a transgenic lethal balb/c mers-cov mouse model we showed that ... | 2017 | 29263883 |
| detection and full genome characterization of two beta cov viruses related to middle east respiratory syndrome from bats in italy. | middle east respiratory syndrome coronavirus (mers-cov), which belongs to beta group of coronavirus, can infect multiple host species and causes severe diseases in humans. multiple surveillance and phylogenetic studies suggest a bat origin. in this study, we describe the detection and full genome characterization of two covs closely related to mers-cov from two italian bats, pipistrellus kuhlii and hypsugo savii. | 2017 | 29258555 |
| international health threats and global early warning and response mechanisms. | the global community continues to incur the high costs of crisis mitigation and emergency response to outbreaks of emerging infectious diseases, such as those caused by the h5n1 highly pathogenic avian influenza virus, ebola virus, nipah virus, zika virus or the middle east respiratory syndrome coronavirus. these viruses are particularly dangerous in regions associated with poor development indicators and high vulnerability. the drivers of these disease crises include failures in the way that an ... | 2017 | 30152454 |
| research on the mechanism of cross-species infection and transmission for animal-origin emerging and re-emerging zoonosis has won support by the national key research and development program of china. | emerging and re-emerging zoonotic diseases caused by pathogens such as middle east respiratory syndrome coronavirus (mers-cov), west nile virus (wnv) or chikungunya virus (chikv) pose considerable threats to public health worldwide. research on the mechanism of cross-species infection and transmission for animal-origin emerging and re-emerging zoonosis (2016yfd0500300) has won support by the national key research and development program of china. professor wenjie tan (national institute for vira ... | 2017 | 30702832 |
| mers-cov infection in a pregnant woman in korea. | middle east respiratory syndrome (mers) is a lethal respiratory disease - caused by mers-coronavirus (mers-cov) which was first identified in 2012. especially, pregnant women can be expected as highly vulnerable candidates for this viral infection. in may 2015, this virus was spread in korea and a pregnant woman was confirmed with positive result of mers-cov polymerase chain reaction (pcr). her condition was improved only with conservative treatment. after a full recovery of mers, the patient ma ... | 2017 | 28875620 |
| assessing the detection of middle east respiratory syndrome coronavirus igg in suspected and proven cases of middle east respiratory syndrome coronavirus infection. | middle east respiratory syndrome coronavirus (mers-cov) causes severe respiratory disease with significant mortality. two testing methods are currently used for mers-cov diagnostics: nucleic acid detection (the gold standard) and serological analysis. in this study, we evaluated the detection of mers-cov-igg in suspected and proven cases of mers-cov infection. we enrolled 174 patients: 113 had respiratory symptoms/suspected mers-cov infection, 31 had confirmed influenza a or b infection, 23 had ... | 2017 | 28873020 |
| a multi-faceted approach of a nursing led education in response to mers-cov infection. | the emergence of the middle east respiratory syndrome-coronavirus (mers-cov) resulted in multiple healthcare associated outbreaks. in response, we developed a nurse-led program to screen and triage patients with mers-cov infection. | 2017 | 28869153 |
| newcastle disease virus-based mers-cov candidate vaccine elicits high-level and lasting neutralizing antibodies in bactrian camels. | middle east respiratory syndrome coronavirus (mers-cov), a member of the coronaviridae family, is the causative pathogen for mers that is characterized by high fever, pneumonia, acute respiratory distress syndrome (ards), as well as extrapulmonary manifestations. currently, there are no approved treatment regimens or vaccines for mers. here, we generated recombinant nonvirulent newcastle disease virus (ndv) lasota strain expressing mers-cov s protein (designated as rla-mers-s), and evaluated its ... | 2017 | 32288953 |
| emerging developments on pathogenicity, molecular virulence, epidemiology and clinical symptoms of current middle east respiratory syndrome coronavirus (mers-cov). | middle east respiratory syndrome coronavirus (mers-cov) is a recently reported virus that is associated with severe, life threatening and rapidly spreading primarily respiratory illness called the middle east respiratory syndrome. mers-cov possesses a unique positive-sense single-stranded rna and can undergo rapid mutation in the viral genome. this results in antigenic switching and genetic variation, finally leading to the emergence of novel and new mers-cov subtypes which are uncontrollable by ... | 2017 | 32288937 |
| biosafety and biosecurity in veterinary laboratories. | with recent outbreaks of middle east respiratory syndrome coronavirus (mers-cov), anthrax, nipah and the highly pathogenic avian influenza virus, much emphasis has been placed on the rapid identification of infectious agents globally. as a result, laboratories are building capacity, conducting more advanced and sophisticated research, increasing their staff, and establishing reference collections of dangerous pathogens in an attempt to reduce the impact of infectious disease outbreaks and to cha ... | 2017 | 30152450 |
| application of a quantitative entry assessment model to compare the relative risk of incursion of zoonotic bat-borne viruses into european union member states. | this paper presents a quantitative assessment model for the risk of entry of zoonotic bat-borne viruses into the european union (eu). the model considers four routes of introduction: human travel, legal trade of products, live animal imports and illegal import of bushmeat and was applied to five virus outbreak scenarios. two scenarios were considered for zaire ebolavirus (webov, cebov) and other scenarios for hendra virus, marburg virus (marv) and middle east respiratory syndrome coronavirus (me ... | 2017 | 32289058 |
| influenza and other emerging respiratory viruses. | acute respiratory infections are one of the top five causes of mortality worldwide and contribute to >4 million deaths per year. consequently, emerging respiratory viruses are a continuing threat to global health security and have the potential to affect our economies. since the millennium, there have been around a dozen different outbreaks, several capturing international interest. the outbreak of severe acute respiratory syndrome coronavirus saw the beginning of an extensive global collaborati ... | 2017 | 32288582 |
| performance of curb-65 in predicting mortality of patients with community-acquired pneumonia in saudi arabia. | various objective scoring systems were developed to standardize the approach to the designation of severity of community-acquired pneumonia (cap). there is limited data on the use of curb-65 among admitted cap patients in saudi arabia. methodology: the retrospective study included cap patients, admitted to a general hospital in eastern saudi arabia. the curb-65 was extracted from the available medical records. | 2017 | 31600155 |
| management of patient flow in vascular and interventional radiology during mers-cov outbreak. | in 2015, an outbreak of middle east respiratory syndrome coronavirus occurred in saudi arabia and necessitated special measures to be implemented to control the spread of the virus. in this article, we will discuss how the outbreak was managed in the vascular and interventional radiology (vir) suite in a large tertiary care hospital in saudi arabia. various measures were taken to reduce the risk of transmission of infection. unit-level education played an important role in the care of patients. ... | 2017 | 32288661 |
| a novel antiviral strategy against mers-cov and hcov-229e using binase to target viral genome replication. | rna viruses cause most of the dangerous communicable diseases. due to their high mutation rates, rna viruses quickly evade selective pressures and can adapt to a new host. therefore, new antiviral approaches are urgently needed, which target more than one specific virus variant and which would optimally prevent development of viral resistance. among the family of coronaviruses (cov), several human pathogenic strains (hcov) are known to cause respiratory diseases and are implied in enteric diseas ... | 2017 | 32219056 |
| middle east respiratory syndrome coronavirus - mers, mers cov. | 2017 | 29737284 | |
| drivers of airborne human-to-human pathogen transmission. | airborne pathogens - either transmitted via aerosol or droplets - include a wide variety of highly infectious and dangerous microbes such as variola virus, measles virus, influenza a viruses, mycobacterium tuberculosis, streptococcus pneumoniae, and bordetella pertussis. emerging zoonotic pathogens, for example, mers coronavirus, avian influenza viruses, coxiella, and francisella, would have pandemic potential were they to acquire efficient human-to-human transmissibility. here, we synthesize in ... | 2017 | 27918958 |
| mplex: a method for simultaneous pathogen inactivation and extraction of samples for multi-omics profiling. | the continued emergence and spread of infectious agents is of great concern, and systems biology approaches to infectious disease research can advance our understanding of host-pathogen relationships and facilitate the development of new therapies and vaccines. molecular characterization of infectious samples outside of appropriate biosafety containment can take place only subsequent to pathogen inactivation. herein, we describe a modified folch extraction using chloroform/methanol that facilita ... | 2017 | 28091625 |
| novel chimeric virus-like particles vaccine displaying mers-cov receptor-binding domain induce specific humoral and cellular immune response in mice. | middle east respiratory syndrome coronavirus (mers-cov) has continued spreading since its emergence in 2012 with a mortality rate of 35.6%, and is a potential pandemic threat. prophylactics and therapies are urgently needed to address this public health problem. we report here the efficacy of a vaccine consisting of chimeric virus-like particles (vlp) expressing the receptor binding domain (rbd) of mers-cov. in this study, a fusion of the canine parvovirus (cpv) vp2 structural protein gene with ... | 2017 | 28040513 |
| chikungunya infection presenting as mild encephalitis with a reversible lesion in the splenium: a case report. | chikungunya fever is an aedes mosquito-transmitted infection caused by chikungunya virus, an rna virus in the family togaviridae. the disease is characteristically manifested as fever, arthralgia, and/or rash. various neurological manifestations like meningoencephalitis, myelitis, and myeloneuropathy have been mentioned in various reports. we present a rare case of chikungunya fever presenting with mild encephalitis with a reversible lesion of the splenium (mers), which showed complete clinical ... | 2017 | 28105556 |
| mild encephalitis/encephalopathy with reversible splenial lesion (mers) due to dengue virus. | a 14-year-old girl presented with encephalopathy, delirium and ophthalmoplegia following a 3day history of high-grade fever. brain mri on day 6 of illness showed diffusion restricted ovoid lesion in the splenium of corpus callosum. dengue virus encephalitis was diagnosed with positive pcr for dengue virus type-2 in both serum and cerebrospinal fluid. she made a complete recovery from day 10 of illness. repeat brain mri on day 12 of illness showed resolution of the splenial lesion. serial diffusi ... | 2017 | 27887978 |
| the 17th international congress on infectious diseases workshop on developing infection prevention and control resources for low- and middle-income countries. | hospital-acquired infections (hais) are a major concern to healthcare systems around the world. they are associated with significant morbidity and mortality, in addition to increased hospitalization costs. recent outbreaks, including those caused by the middle east respiratory syndrome coronavirus and ebola virus, have highlighted the importance of infection control. moreover, hais, especially those caused by multidrug-resistant gram-negative rods, have become a top global priority. although ade ... | 2017 | 28216179 |
| one-health: a safe, efficient, dual-use vaccine for humans and animals against middle east respiratory syndrome coronavirus and rabies virus. | middle east respiratory syndrome coronavirus (mers-cov) emerged in 2012 and is a highly pathogenic respiratory virus. there are no treatment options against mers-cov for humans or animals, and there are no large-scale clinical trials for therapies against mers-cov. to address this need, we developed an inactivated rabies virus (rabv) that contains the mers-cov spike (s) protein expressed on its surface. our initial recombinant vaccine, bnsp333-s, expresses a full-length wild-type mers-cov s prot ... | 2017 | 27807241 |
| bcx4430 - a broad-spectrum antiviral adenosine nucleoside analog under development for the treatment of ebola virus disease. | the adenosine nucleoside analog bcx4430 is a direct-acting antiviral drug under investigation for the treatment of serious and life-threatening infections from highly pathogenic viruses, such as the ebola virus. cellular kinases phosphorylate bcx4430 to a triphosphate that mimics atp; viral rna polymerases incorporate the drug's monophosphate nucleotide into the growing rna chain, causing premature chain termination. bcx4430 is active in vitro against many rna viral pathogens, including the filo ... | 2017 | 27095300 |
| structural biology of the arterivirus nsp11 endoribonucleases. | endoribonuclease (nendou) is unique and conserved as a major genetic marker in nidoviruses that infect vertebrate hosts. arterivirus nonstructural protein 11 (nsp11) was shown to have nendou activity and play essential roles in the viral life cycle. here, we report three crystal structures of porcine reproductive and respiratory syndrome virus (prrsv) and equine arteritis virus (eav) nsp11 mutants. the structures of arterivirus nsp11 contain two conserved compact domains: the n-terminal domain ( ... | 2017 | 27795409 |
| coronavirus infections in horses in saudi arabia and oman. | equine coronaviruses (ecov) are the only coronavirus known to infect horses. so far, data on ecov infection in horses remain limited to the usa, france and japan and its geographic distribution is not well understood. we carried out rt-pcr on 306 nasal and 315 rectal swabs and tested 243 sera for antibodies to detect coronavirus infections in apparently healthy horses in saudi arabia and oman. we document evidence of infection with ecov and hku23 coronavirus by rt-pcr. there was no conclusive ev ... | 2017 | 28296228 |
| report from the world health organization's third product development for vaccines advisory committee (pdvac) meeting, geneva, 8-10th june 2016. | the third meeting of who's product development for vaccines advisory committee (pdvac) was held in june 2016, with a remit to revisit the pathogen areas for which significant progress has occurred since recommendations from the 2015 meeting, as well as to consider new advances in the development of vaccines against other pathogens. since the previous meeting, significant progress has been made with regulatory approvals of the first malaria and dengue vaccines, and the first phase iii trials of a ... | 2017 | 28262332 |
| lineage a betacoronavirus ns2 proteins and the homologous torovirus berne pp1a carboxy-terminal domain are phosphodiesterases that antagonize activation of rnase l. | viruses in the family coronaviridae, within the order nidovirales, are etiologic agents of a range of human and animal diseases, including both mild and severe respiratory diseases in humans. these viruses encode conserved replicase and structural proteins as well as more diverse accessory proteins, encoded in the 3' ends of their genomes, that often act as host cell antagonists. we previously showed that 2',5'-phosphodiesterases (2',5'-pdes) encoded by the prototypical betacoronavirus, mouse he ... | 2017 | 28003490 |
| influenza not mers cov among returning hajj and umrah pilgrims with respiratory illness, kashmir, north india, 2014-15. | the increasing reports of middle east respiratory syndrome (mers) caused by mers coronavirus (mers-cov) from many countries emphasize its importance for international travel. muslim pilgrimages of hajj and umrah involve mass gatherings of international travellers. we set out to assess the presence of influenza and mers-cov in hajj/umrah returnees with acute respiratory infection. . | 2017 | 27932291 |
| surveillance of bat coronaviruses in kenya identifies relatives of human coronaviruses nl63 and 229e and their recombination history. | bats harbor a large diversity of coronaviruses (covs), several of which are related to zoonotic pathogens that cause severe disease in humans. our screening of bat samples collected in kenya from 2007 to 2010 not only detected rna from several novel covs but, more significantly, identified sequences that were closely related to human covs nl63 and 229e, suggesting that these two human viruses originate from bats. we also demonstrated that human cov nl63 is a recombinant between nl63-like viruses ... | 2017 | 28077633 |
| clinical isolates of human coronavirus 229e bypass the endosome for cell entry. | human coronavirus 229e (hcov-229e), a causative agent of the common cold, enters host cells via two distinct pathways: one is mediated by cell surface proteases, particularly transmembrane protease serine 2 (tmprss2), and the other by endosomal cathepsin l. thus, specific inhibitors of these proteases block virus infection. however, it is unclear which of these pathways is actually utilized by hcov-229e in the human respiratory tract. here, we examined the mechanism of cell entry used by a pseud ... | 2017 | 27733646 |
| acute viral respiratory infections among children in mers-endemic riyadh, saudi arabia, 2012-2013. | the emergence of the middle east respiratory syndrome (mers) in saudi arabia has intensified focus on acute respiratory infections [aris]. this study sought to identify respiratory viruses (rvs) associated with aris in children presenting at a tertiary hospital. children (aged ≤13) presenting with ari between january 2012 and december 2013 tested for 15 rvs using the seeplex(r) rv15 kit were retrospectively included. epidemiological data was retrieved from patient records. of the 2235 children t ... | 2017 | 27430485 |
| hajj-associated viral respiratory infections: a systematic review. | respiratory tract infections (rti) are the most common infections transmitted between hajj pilgrims. the aim of this systematic review was to determine the prevalence of virus carriage potentially responsible for rti among pilgrims before and after participating in the hajj. a systematic search for relevant literature was conducted according to preferred reporting items for systematic reviews and meta-analyses guidelines. 31 studies were identified. severe acute respiratory syndrome coronavirus ... | 2017 | 26781223 |
| patterns of human respiratory viruses and lack of mers-coronavirus in patients with acute upper respiratory tract infections in southwestern province of saudi arabia. | we undertook enhanced surveillance of those presenting with respiratory symptoms at five healthcare centers by testing all symptomatic outpatients between november 2013 and january 2014 (winter time). nasal swabs were collected from 182 patients and screened for mers-cov as well as other respiratory viruses using rt-pcr and multiplex microarray. a total of 75 (41.2%) of these patients had positive viral infection. mers-cov was not detected in any of the samples. human rhinovirus (hrv) was the mo ... | 2017 | 28348590 |
| creating an artificial tail anchor as a novel strategy to enhance the potency of peptide-based hiv fusion inhibitors. | 20 (enfuvirtide) and other peptides derived from the human immunodeficiency virus type 1 (hiv-1) gp41 c-terminal heptad repeat (chr) region inhibit hiv fusion by binding to the hydrophobic grooves on the n-terminal heptad repeat (nhr) trimer and blocking six-helix-bundle (6-hb) formation. several strategies focusing on the binding grooves of the nhr trimer have been adopted to increase the antiviral activity of the chr peptides. here, we developed a novel and simple strategy to greatly enhance t ... | 2017 | 27795416 |
| using reference-free compressed data structures to analyze sequencing reads from thousands of human genomes. | we are rapidly approaching the point where we have sequenced millions of human genomes. there is a pressing need for new data structures to store raw sequencing data and efficient algorithms for population scale analysis. current reference-based data formats do not fully exploit the redundancy in population sequencing nor take advantage of shared genetic variation. in recent years, the burrows-wheeler transform (bwt) and fm-index have been widely employed as a full-text searchable index for read ... | 2017 | 27986821 |
| the impact of co-infection of influenza a virus on the severity of middle east respiratory syndrome coronavirus. | 2017 | 28189714 | |
| human lung ex vivo infection models. | pneumonia is counted among the leading causes of death worldwide. viruses, bacteria and pathogen-related molecules interact with cells present in the human alveolus by numerous, yet poorly understood ways. traditional cell culture models little reflect the cellular composition, matrix complexity and three-dimensional architecture of the human lung. integrative animal models suffer from species differences, which are of particular importance for the investigation of zoonotic lung diseases. the us ... | 2017 | 27999962 |
| neurovirulent murine coronavirus jhm.sd uses cellular zinc metalloproteases for virus entry and cell-cell fusion. | the coronavirus (cov) s protein requires cleavage by host cell proteases to mediate virus-cell and cell-cell fusion. many strains of the murine coronavirus mouse hepatitis virus (mhv) have distinct, s-dependent organ and tissue tropisms despite using a common receptor, suggesting that they employ different cellular proteases for fusion. in support of this hypothesis, we found that inhibition of endosomal acidification only modestly decreased entry, and overexpression of the cell surface protease ... | 2017 | 28148786 |
| evolutionary mechanism and biological functions of 8-mers containing cg dinucleotide in yeast. | the rules of k-mer non-random usage and the biological functions are worthy of special attention. firstly, the article studied human 8-mer spectra and found that only the spectra of cytosine-guanine (cg) dinucleotide classification formed independent unimodal distributions when the 8-mers were classified into three subsets under 16 dinucleotide classifications. secondly, the distribution rules were reproduced by other seven species including yeast, which showed that the evolution phenomenon had ... | 2017 | 28181048 |
| the severe acute respiratory syndrome coronavirus nucleocapsid inhibits type i interferon production by interfering with trim25-mediated rig-i ubiquitination. | severe acute respiratory syndrome (sars) is a respiratory disease, caused by a coronavirus (sars-cov), that is characterized by atypical pneumonia. the nucleocapsid protein (n protein) of sars-cov plays an important role in inhibition of type i interferon (ifn) production via an unknown mechanism. in this study, the sars-cov n protein was found to bind to the spry domain of the tripartite motif protein 25 (trim25) e3 ubiquitin ligase, thereby interfering with the association between trim25 and r ... | 2017 | 28148787 |
| do medical students receive training in correct use of personal protective equipment? | healthcare personnel often use incorrect technique for donning and doffing of personal protective equipment (ppe). | 2017 | 28178912 |
| vaccine development for emerging virulent infectious diseases. | the recent outbreak of zaire ebola virus in west africa altered the classical paradigm of vaccine development and that for emerging infectious diseases (eids) in general. in this paper, the precepts of vaccine discovery and advancement through pre-clinical and clinical assessment are discussed in the context of the recent ebola virus, middle east respiratory syndrome coronavirus (mers-cov), and zika virus outbreaks. clinical trial design for diseases with high mortality rates and/or high morbidi ... | 2017 | 28216184 |
| preface - emerging viruses: from early detection to intervention. | in the last decades, several viruses emerged, as the consequence of cross-species passage from animal reservoirs to human being. emerging infections always represent a significant challenge for public health system as knowledge about specific pathogens are generally little while and no/few effective interventions are available. in this volume we explored special aspects of emerging infectious diseases including: the application of the theory of focality of diseases to infective syndrome; the hum ... | 2017 | 28364363 |
| an isothermal, label-free, and rapid one-step rna amplification/detection assay for diagnosis of respiratory viral infections. | recently, rna viral infections caused by respiratory viruses, such as influenza, parainfluenza, respiratory syncytial virus, coronavirus, and middle east respiratory syndrome-coronavirus (mers-cov), and zika virus, are a major public health threats in the world. although myriads of diagnostic methods based on rna amplification have been developed in the last decades, they continue to lack speed, sensitivity, and specificity for clinical use. a rapid and accurate diagnostic method is needed for a ... | 2017 | 27894035 |
| rapid generation of a human monoclonal antibody to combat middle east respiratory syndrome. | the last century has witnessed the emergence of several previously unknown viruses as life-threatening human pathogens. several examples include hiv, ebola, lujo, and, most recently, the middle east respiratory syndrome (mers) and ebola. in this study, we describe a method for the swift generation of a human-derived monoclonal antibody, known as lca60, as a treatment for mers infections. lca60 antibody was generated using the cellclone technology from the immortalized b cells of a human donor re ... | 2017 | 27102927 |
| high prevalence of middle east respiratory coronavirus in young dromedary camels in jordan. | prevalence of middle east respiratory syndrome coronavirus (mers-cov) was determined in 45 dromedary camels from two geographically separated herds in jordan. virus shedding was only detected in swabs obtained from the respiratory tract and primarily observed in camels younger than 3 years. mers-cov seroprevalence increased with age of camels. bovine and sheep sera were seronegative. phylogenetic analysis of partial s2 clustered the jordanian mers-cov strains with contemporary mers-cov strains a ... | 2017 | 28009529 |
| vaccines against middle east respiratory syndrome coronavirus for humans and camels. | middle east respiratory syndrome coronavirus (mers-cov) is caused by a novel betacoronavirus that was isolated in late 2012 in saudi arabia. the viral infections have been reported in more than 1700 humans, ranging from asymptomatic or mild cases to severe pneumonia with a mortality rate of 40%. it is well documented now that dromedary camels contract the infection and shed the virus without notable symptoms, and such animals had been infected by at least the early 1980s. the mechanism of camel ... | 2017 | 27786402 |
| efficacy of antibody-based therapies against middle east respiratory syndrome coronavirus (mers-cov) in common marmosets. | cases of middle east respiratory syndrome coronavirus (mers-cov) continue to be identified and with a lack of effective clinical treatment and no preventative strategies, treatment using convalescent plasma or monoclonal antibodies (mabs) is a potential quick route to an intervention. passive immunotherapy via either convalescent plasma or mabs has proven to be effective for other infectious agents. following infection with mers-cov, common marmosets were treated with high titer hyperimmune plas ... | 2017 | 28389142 |
| comparative pathology of rhesus macaque and common marmoset animal models with middle east respiratory syndrome coronavirus. | middle east respiratory syndrome (mers), which is caused by a newly discovered coronavirus (cov), has recently emerged. it causes severe viral pneumonia and is associated with a high fatality rate. however, the pathogenesis, comparative pathology and inflammatory cell response of rhesus macaques and common marmosets experimentally infected with mers-cov are unknown. we describe the histopathological, immunohistochemical, and ultrastructural findings from rhesus macaque and common marmoset animal ... | 2017 | 28234937 |
| identification of risk factors for primary mers-cov infection in camel workers in qatar, 2013 - 2014; a case-control study. | the transmission routes and risk factors for zoonotic mers-cov infections are still unknown. we used the who questionnaire for mers-cov case-control studies to assess risk factors for human mers-cov seropositivity at a farm complex in qatar. nine camel workers with mers-cov antibodies and 43 workers without antibodies were included. some camel-related activities may pose a higher risk of mers-cov infection, as well as cross-border movements of camels, poor hand hygiene and overnight hospital sta ... | 2017 | 28387845 |
| risk factors for mers coronavirus infection in dromedary camels in burkina faso, ethiopia, and morocco, 2015. | understanding middle east respiratory syndrome coronavirus (mers-cov) transmission in dromedary camels is important, as they consitute a source of zoonotic infection to humans. to identify risk factors for mers-cov infection in camels bred in diverse conditions in burkina faso, ethiopia and morocco, blood samples and nasal swabs were sampled in february-march 2015. a relatively high mers-cov rna rate was detected in ethiopia (up to 15.7%; 95% confidence interval (ci): 8.2-28.0), followed by burk ... | 2017 | 28382915 |
| cross-sectional surveillance of middle east respiratory syndrome coronavirus (mers-cov) in dromedary camels and other mammals in egypt, august 2015 to january 2016. | a cross-sectional study was conducted in egypt to determine the prevalence of middle east respiratory syndrome coronavirus (mers-cov) in imported and resident camels and bats, as well as to assess possible transmission of the virus to domestic ruminants and equines. a total of 1,031 sera, 1,078 nasal swabs, 13 rectal swabs, and 38 milk samples were collected from 1,078 camels in different types of sites. in addition, 145 domestic animals and 109 bats were sampled. overall, of 1,031 serologically ... | 2017 | 28333616 |
| immunogenicity of candidate mers-cov dna vaccines based on the spike protein. | mers-coronavirus is a novel zoonotic pathogen which spread rapidly to >25 countries since 2012. its apparent endemicity and the wide spread of its reservoir host (dromedary camels) in the arabian peninsula highlight the ongoing public health threat of this virus. therefore, development of effective prophylactic vaccine needs to be urgently explored given that there are no approved prophylactics or therapeutics for humans or animals to date. different vaccine candidates have been investigated but ... | 2017 | 28332568 |
| serologic evidence for mers-cov infection in dromedary camels, punjab, pakistan, 2012-2015. | dromedary camels from africa and arabia are an established source for zoonotic middle east respiratory syndrome coronavirus (mers-cov) infection among humans. in pakistan, we found specific neutralizing antibodies in samples from 39.5% of 565 dromedaries, documenting significant expansion of the enzootic range of mers-cov to asia. | 2017 | 28221127 |
| middle east respiratory syndrome (mers) coronavirus and dromedaries. | middle east respiratory syndrome (mers) is a zoonotic viral disease that can be transmitted from dromedaries to human beings. more than 1500 cases of mers have been reported in human beings to date. although mers has been associated with 30% case fatality in human beings, mers coronavirus (mers-cov) infection in dromedaries is usually asymptomatic. in rare cases, dromedaries may develop mild respiratory signs. no mers-cov or antibodies against the virus have been detected in camelids other than ... | 2017 | 28190501 |
| outbreak of middle east respiratory syndrome coronavirus in saudi arabia: a retrospective study. | the middle east respiratory syndrome (mers) is proposed to be a zoonotic disease. dromedary camels have been implicated due to reports that some confirmed cases were exposed to camels. risk factors for mers coronavirus (mers-cov) infections in humans are incompletely understood. this study aimed to describe the demographic characteristics, mortality rate, clinical manifestations and comorbidities with confirmed cases of mers-cov. | 2017 | 28056850 |
| systematic, active surveillance for middle east respiratory syndrome coronavirus in camels in egypt. | middle east respiratory syndrome coronavirus (mers-cov) causes severe human infections and dromedary camels are considered an intermediary host. the dynamics of natural infection in camels are not well understood. through systematic surveillance in egypt, nasal, rectal, milk, urine and serum samples were collected from camels between june 2014 and february 2016. locations included quarantines, markets, abattoirs, free-roaming herds and farmed breeding herds. the overall seroprevalence was 71% an ... | 2017 | 28050021 |
| dromedary camels and the transmission of middle east respiratory syndrome coronavirus (mers-cov). | middle east respiratory syndrome coronavirus (mers-cov) is an existential threat to global public health. the virus has been repeatedly detected in dromedary camels (camelus dromedarius). adult animals in many countries in the middle east as well as in north and east africa showed high (>90%) seroprevalence to the virus. middle east respiratory syndrome coronavirus isolated from dromedaries is genetically and phenotypically similar to viruses from humans. we summarize current understanding of th ... | 2017 | 26256102 |
| livestock susceptibility to infection with middle east respiratory syndrome coronavirus. | middle east respiratory syndrome (mers) cases continue to be reported, predominantly in saudi arabia and occasionally other countries. although dromedaries are the main reservoir, other animal species might be susceptible to mers coronavirus (mers-cov) infection and potentially serve as reservoirs. to determine whether other animals are potential reservoirs, we inoculated mers-cov into llamas, pigs, sheep, and horses and collected nasal and rectal swab samples at various times. the presence of m ... | 2017 | 27901465 |
| genetic diversity of coronaviruses in bats in lao pdr and cambodia. | south-east asia is a hot spot for emerging zoonotic diseases, and bats have been recognized as hosts for a large number of zoonotic viruses such as severe acute respiratory syndrome (sars), responsible for acute respiratory syndrome outbreaks. thus, it is important to expand our knowledge of the presence of viruses in bats which could represent a risk to humans. coronaviruses (covs) have been reported in bat species from thailand, china, indonesia, taiwan and the philippines. however no such wor ... | 2017 | 27932284 |
| highly sensitive escherichia coli shear horizontal surface acoustic wave biosensor with silicon dioxide nanostructures. | surface acoustic wave mediated transductions have been widely used in the sensors and actuators applications. in this study, a shear horizontal surface acoustic wave (shsaw) was used for the detection of food pathogenic escherichia coli o157:h7 (e.coli o157:h7), a dangerous strain among 225 e. coli unique serotypes. a few cells of this bacterium are able to cause young children to be most vulnerable to serious complications. presence of higher than 1cfu e.coli o157:h7 in 25g of food has been con ... | 2017 | 27660016 |
| broad-spectrum inhibition of common respiratory rna viruses by a pyrimidine synthesis inhibitor with involvement of the host antiviral response. | our previous screening of 50 240 structurally diverse compounds led to the identification of 39 influenza a virus infection inhibitors (kao r.y., yang d., lau l.s., tsui w.h., hu l. et al. nat biotechnol 2010;28:600-605). further screening of these compounds against common respiratory viruses led to the discovery of compound fa-613. this inhibitor exhibited low micromolar antiviral activity against various influenza a and b virus strains, including the highly pathogenic influenza a strains h5n1 ... | 2017 | 28555543 |
| offering patients more: how the west africa ebola outbreak can shape innovation in therapeutic research for emerging and epidemic infections. | although, after an epidemic of over 28 000 cases, there are still no licensed treatments for ebola virus disease (evd), significant progress was made during the west africa outbreak. the pace of pre-clinical development was exceptional and a number of therapeutic clinical trials were conducted in the face of considerable challenges. given the on-going risk of emerging infectious disease outbreaks in an era of unprecedented population density, international travel and human impact on the environm ... | 2017 | 28396467 |
| the tetraspanin cd9 facilitates mers-coronavirus entry by scaffolding host cell receptors and proteases. | infection by enveloped coronaviruses (covs) initiates with viral spike (s) proteins binding to cellular receptors, and is followed by proteolytic cleavage of receptor-bound s proteins, which prompts s protein-mediated virus-cell membrane fusion. infection therefore requires close proximity of receptors and proteases. we considered whether tetraspanins, scaffolding proteins known to facilitate cov infections, hold receptors and proteases together on cell membranes. using knockout cell lines, we f ... | 2017 | 28759649 |
| spatial modelling of contribution of individual level risk factors for mortality from middle east respiratory syndrome coronavirus in the arabian peninsula. | middle east respiratory syndrome coronavirus is a contagious respiratory pathogen that is contracted via close contact with an infected subject. transmission of the pathogen has occurred through animal-to-human contact at first followed by human-to-human contact within families and health care facilities. | 2017 | 28759623 |
| neurological complications during treatment of middle east respiratory syndrome. | middle east respiratory syndrome (mers) has a high mortality rate and pandemic potential. however, the neurological manifestations of mers have rarely been reported since it first emerged in 2012. | 2017 | 28748673 |
| permissivity of dpp4 orthologs to mers-coronavirus is governed by glycosylation and other complex determinants. | middle east respiratory syndrome coronavirus (mers-cov) utilizes dipeptidyl peptidase 4 (dpp4) as an entry receptor. while bat, camel, and human dpp4 support mers-cov infection, several dpp4 orthologs, including mouse, ferret, hamster, and guinea pig, do not. previous work revealed that glycosylation of mouse dpp4 plays a role in blocking mers-cov infection. here, we test whether glycosylation also acts as a determinant of permissivity for ferret, hamster, and guinea pig dpp4. we find that while ... | 2017 | 28747502 |
| outbreak of middle east respiratory syndrome-coronavirus causes high fatality after cardiac operations. | middle east respiratory syndrome-coronavirus (mers-cov) resembles a severe form of community-acquired pneumonia initially reported in saudi arabia in 2012. the mers-cov epidemic poses a big challenge because of its high mortality. in january 2015, a patient who was potentially incubating mers-cov arrived from the emergency department of another hospital and was admitted with acute coronary syndrome. this resulted in an outbreak in the cardiac surgery ward that caused the deaths of 5 of 6 patient ... | 2017 | 28734432 |
| awareness among a saudi arabian university community of middle east respiratory syndrome coronavirus following an outbreak. | this study aimed to measure the level of awareness of faculty members, staff and students in a female saudi arabian university community towards middle east respiratory syndrome coronvirus (mers-cov) following an outbreak. a self-administered questionnaire containing knowledge questions was distributed and completed by the 1541 participants. the overall knowledge score was 43.2%. it was only significantly higher in participants from the health colleges (50.6%). the majority (78.9%) of the respon ... | 2017 | 28730588 |
| diagnostic delays in 537 symptomatic cases of middle east respiratory syndrome coronavirus infection in saudi arabia. | although the literature indicates that patient delays in seeking medical support for middle east respiratory syndrome coronavirus (mers-cov) infections are associated with poor clinical outcomes, delays in the diagnosis itself remain poorly understood in these patients. this study aimed to determine the median time interval from symptom onset to a confirmed diagnosis and to identify the potential predictors of this interval in saudi arabian mers patients. | 2017 | 28728926 |
| the use of xenosurveillance to detect human bacteria, parasites, and viruses in mosquito bloodmeals. | infectious disease surveillance is hindered by several factors, including limited infrastructure and geographic isolation of many resource-poor regions. in addition, the complexities of sample acquisition, processing, and analysis, even in developed regions, can be rate limiting. therefore, new strategies to survey human populations for emerging pathogens are necessary. xenosurveillance is a method that utilizes mosquitoes as sampling devices to search for genetic signatures of pathogens in vert ... | 2017 | 28722623 |
| no serologic evidence of middle east respiratory syndrome coronavirus infection among camel farmers exposed to highly seropositive camel herds: a household linked study, kenya, 2013. | abstracthigh seroprevalence of middle east respiratory syndrome coronavirus (mers-cov) among camels has been reported in kenya and other countries in africa. to date, the only report of mers-cov seropositivity among humans in kenya is of two livestock keepers with no known contact with camels. we assessed whether persons exposed to seropositive camels at household level had serological evidence of infection. in 2013, 760 human and 879 camel sera were collected from 275 and 85 households respecti ... | 2017 | 28719257 |
| impact of middle east respiratory syndrome outbreak on the use of emergency medical resources in febrile patients. | outbreaks of transmissible respiratory infection are suspected to have significant effects on the health of pediatric and geriatric patients. the objective was to assess the impact of the middle east respiratory syndrome (mers) outbreak on the use of emergency resources. | 2017 | 28717779 |
| the clinical and virological features of the first imported case causing mers-cov outbreak in south korea, 2015. | in 2015, the largest outbreak of middle east respiratory syndrome coronavirus (mers-cov) infection outside the middle east occurred in south korea. we summarized the epidemiological, clinical, and laboratory findings of the first korean case of mers-cov and analyzed whole-genome sequences of mers-cov derived from the patient. | 2017 | 28709419 |
| suggested new breakpoints of anti-mers-cov antibody elisa titers: performance analysis of serologic tests. | to provide optimal cut-off values of anti-middle east respiratory syndrome coronavirus (mers-cov) serologic tests, we evaluated performance of elisa igg, elisa iga, ifa igm, and ifa igg using 138 serum samples of 49 mers-cov-infected patients and 219 serum samples of 219 rrt-pcr-negative mers-cov-exposed healthcare personnel and patients. the performance analysis was conducted for two different purposes: (1) prediction of neutralization activity in mers-cov-infected patients, and (2) epidemiolog ... | 2017 | 28695355 |
| middle east respiratory syndrome coronavirus and pulmonary tuberculosis coinfection: implications for infection control. | coinfection of middle east respiratory syndrome coronavirus (mers-cov) with tuberculosis (tb) has not been previously reported. here, we present 2 cases with both mers-cov and pulmonary tb. the first case was a 13-year-old patient who was admitted with a 2-month history of fever, weight loss, night sweats, and cough. the second patient was a 30-year-old female who had a 4-week history of cough associated with shortness of breath and weight loss of 2 kg. the 2 patients were diagnosed with pulmona ... | 2017 | 28683463 |
| middle east respiratory syndrome clinical practice guideline for hemodialysis facilities. | the korean society of nephrology participated in the task force team consisting of government authorities and civilian experts to prevent and control the spread of middle east respiratory syndrome (mers) in 2015. the korean society of nephrology mers task force team took an immediate action and drafted 'the clinical recommendation for hemodialysis facilities' to follow when the first and the only confirmed case was reported in the hemodialysis unit. owing to the dedicated support from medical do ... | 2017 | 28680819 |
| middle east respiratory syndrome coronavirus experimental transmission using a pig model. | dromedary camels are the main reservoir of middle east respiratory syndrome coronavirus (mers-cov), but other livestock species (i.e., alpacas, llamas, and pigs) are also susceptible to infection with mers-cov. animal-to-animal transmission in alpacas was reported, but evidence for transmission in other species has not been proved. this study explored pig-to-pig mers-cov transmission experimentally. virus was present in nasal swabs of infected animals, and limited amounts of viral rna, but no in ... | 2017 | 28653496 |
| crystal structure of middle east respiratory syndrome coronavirus helicase. | middle east respiratory syndrome coronavirus (mers-cov) remains a threat to public health worldwide; however, effective vaccine or drug against covs remains unavailable. cov helicase is one of the three evolutionary most conserved proteins in nidoviruses, thus making it an important target for drug development. we report here the first structure of full-length coronavirus helicase, mers-cov nsp13. mers-cov helicase has multiple domains, including an n-terminal cys/his rich domain (ch) with three ... | 2017 | 28651017 |
| mers-cov infection: mind the public knowledge gap. | in august 2015, the corona outbreak caused by middle east respiratory syndrome coronavirus (mers-cov) was the 9th episode since june 2012 in saudi arabia. little is known about the public awareness toward the nature or prevention of the disease. the aim of this work was to assess the knowledge of the adult population in riyadh toward the mers-cov. in this cross-sectional survey, a self-administrated questionnaire was distributed to randomly selected participants visiting malls in riyadh. the que ... | 2017 | 28647126 |
| discovery of a highly divergent coronavirus in the asian house shrew from china illuminates the origin of the alphacoronaviruses. | although shrews are one of the largest groups of mammals little is known about their role in the evolution and transmission of viral pathogens including coronaviruses. we captured 266 asian house shrews (suncus murinus) in jiangxi and zhejiang provinces, china, during 2013-2015. coronavirus (cov) rna was detected in 24 asian house shrews, with an overall prevalence of 9.02%. complete viral genome sequences were successfully recovered from the rna positive samples. the newly discovered shrew cov ... | 2017 | 28637760 |
| longitudinal study of middle east respiratory syndrome coronavirus infection in dromedary camel herds in saudi arabia, 2014-2015. | two herds of dromedary camels were longitudinally sampled with nasal and rectal swabs and serum, between september 2014 and may 2015, and the samples were tested for middle east respiratory syndrome (mers) coronavirus rna and antibodies. evidence of mers-cov infection was confirmed in one herd on the basis of detection of virus rna in nasal swabs from three camels and significant increases in the antibody titers from three others. the three viruses were genetically identical, thus indicating int ... | 2017 | 28634355 |
| dynamics of scientific publications on the mers-cov outbreaks in saudi arabia. | middle east respiratory syndrome coronavirus (mers-cov) is an emerging disease with a relatively high case fatality rate. most cases have been reported from saudi arabia, and the disease epidemic potential is considered to be limited. however, human-human transmission has occurred, usually in the context of healthcare facility-associated outbreaks. the scientific and medical community depends on timely publication of epidemiological information on emerging diseases during outbreaks to appropriat ... | 2017 | 28625842 |
| dromedary camels in northern mali have high seropositivity to mers-cov. | a high percentage (up to 90%) of dromedary camels in the middle east as well as eastern and central africa have antibodies to middle east respiratory syndrome coronavirus (mers-cov). here we report comparably high positivity of mers-cov antibodies in dromedary camels from northern mali. this extends the range of mers-cov further west in africa than reported to date and cautions that mers-cov should be considered in cases of severe respiratory disease in the region. | 2017 | 28616502 |