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site of interaction of serotonin and desglycinamide-arginine-vasopressin in maintenance of ethanol tolerance.rats trained to walk in a moving belt apparatus were subjected to a partial (fornix-fimbria (ff] or total (fornix-fimbria + cingulum bundles (ff + cb] chemical denervation of the dorsal serotonergic afferent pathways to the hippocampus. after chronic alcohol treatment that resulted in tolerance development to the motor-impairing effects of ethanol, desglycinamide-arginine8-vasopressin (dgavp) or saline treatment was started and the residual tolerance measured at several intervals after ethanol w ...19854038620
spatial distribution of retinol-binding protein and retinyl palmitate hydrolase activity in normal and vitamin a-deficient rat liver.a study was conducted to explore the spatial distribution within rat liver of two proteins importantly involved in retinoid metabolism in liver, namely, retinol-binding protein (rbp) and the enzyme retinyl palmitate hydrolase (rph). the study was conducted with both vitamin a-sufficient (control) and vitamin a-deficient rats. livers were carefully and reproducibly dissected into 11 sections each, and rbp levels and rph activities were measured for each section homogenate. both rbp and rph activi ...19854040159
sow (sus scrofa) follicular fluid: prostaglandin content and effect on the motility of isolated oviducts.the present study provides information regarding the effects of the sow follicular fluid (ff) on the motility of isolated segments of swine and rabbit oviducts. in addition, the concentration of prostaglandins (pgs) f2 alpha, e2 and e1 in the follicular fluid of sow ovaries isolated at different stages of the sex cycle as well as the generation of the same pgs by walls of ovarian follicles in early and late proestrus, in estrus, in metestrus and in diestrus, were explored. the stimulatory contra ...19854040260
local cerebral glucose utilization in normal female rats: variations during the estrous cycle and comparison with males.the quantitative 2-[14c]deoxyglucose autoradiographic method was used to study the fluctuations of energy metabolism in discrete brain regions of female rats during the estrous cycle. a consistent though statistically nonsignificant cyclic variation in average glucose utilization of the brain as a whole was observed. highest levels of glucose utilization occurred during proestrus and metestrus, whereas lower rates were found during estrus and diestrus. statistically significant fluctuations were ...19854040921
periosteal stress-induced reactions resembling stress fractures. a radiologic and histologic study in dogs.an external callus is always associated with so-called stress fractures, but a fracture line cannot always be demonstrated by radiologic means. in such instances it is assumed that an undisplaced fracture or microfracture must nevertheless exist for an external callus to form. in this experimental study, 18 beagles were immobilized in a shoulder spica for periods of time ranging from 6 to 32 weeks and then remobilized. at the time of sacrifice, which varied from four to 28 weeks after remobiliza ...19854042491
cryptococcal meningitis: presentation as sudden deafness.a patient presented with a bilateral profound hearing loss of sudden onset following a two-month neurologic illness. microscopy and culture of cerebrospinal fluid revealed cryptococcus neoformans. treatment with amphotericin b and 5-fluorocytosine failed to restore hearing. auditory brain stem response and electrical promontory stimulation suggest a profound deafness with poor neuronal survival. this is consistent with previous temporal bone histopathology reports in individuals dying of cryptoc ...19854050983
laminar origin of direct projection from cortex area v1 to v4 in the rhesus monkey.direct projection from cortex area v1 to v4 in the rhesus monkey was demonstrated by means of retrograde axonal transport of wheat germ agglutinin-conjugated horseradish peroxidase (wga-hrp). this projection originated at the layers 2 and 3 of the only representation area of central visual field (0-6 degrees) in v1 and terminated at the central representation of v4, whereas there was no projection between the extracentral representations of v1 and v4. correlation of the present finding with the ...19854052788
drastic improvement in the rectal absorption profile of morphine in man.rectal absorption of morphine hcl from aqueous vehicles at different phs in man has been compared with an orally administered solution. plasma concentrations of morphine were measured by electrochemical hplc analysis after a single dose of 10 mg morphine hcl, in a cross-over study in 7 volunteers. rectal absorption of morphine was dependent on ph, which could be explained as being due to ph partitioning. the absorption rate and bioavailability could be greatly improved, as compared to orally adm ...19854054201
[reactions of hypothalamic neurons to stimulation of the pelvic and sciatic nerves].unit activity of posterior, tuberal and anterior parts of the hypothalamus was studied during stimulation of the pelvic nerve in anesthetized and immobilized cats. comparative analysis showed that neurons of the posterior hypothalamus were more responsive (89.6%) and had a shorter mean latency (33.3 +/- 2.4 msec) than neurons of more rostral parts of the hypothalamus. the focus of maximal pelvic-induced unit activity seems to be located in the posterior hypothalamus. the unit responses of the po ...19854054386
[dna synthesis in cultures of atrial and ventricular cardiomyocytes in the rat].by means of 3h-thymidine autoradiography dna replicative activity has been studied in cultured atrial and ventricular myocytes, and non-muscle cells from hearts of 2-week-old rats (age when cell proliferation in the myocardium is already significantly depressed). pas-reaction was used as a cytochemical marker of cardiomyocytes: atrial myocytes are richer in glycogen than ventricular cells. labeling indices of atrial myocytes after a 24 hour exposure to 3h-thymidine were higher than ventricular o ...19854060232
age-associated alterations in hepatic glutathione-s-transferase activities.age-associated alterations of hepatic cytosolic glutathione-s-transferase (gst) activities towards sulfobromophthalein sodium tetrahydrate (bsp), styrene oxide (stox), trans-4-phenyl-3-butene-2-one (pbo), 1,2-dichloro-4-nitrobenzene (dcnb), and 1-chloro-2,4-dinitrobenzene (cdnb) were investigated in fischer-344 rats of both sexes with ages ranging from 1.5 to 28 months. the gst activities towards pbo and dcnb in male rats increased with age till 6-12 months when maximum values were attained, and ...19854062964
effect of detergent on protein structure. action of detergents on secondary and oligomeric structures of band 3 from bovine erythrocyte membranes.with special interest in the mode of action of zwitterionic detergents on proteins, a variety of detergents were examined for their ability to disrupt the secondary and quaternary structures of an anion transport protein, band 3, and its cytoplasmic 38 kda fragment from bovine erythrocyte membranes and for their effect on the binding of an anion transport inhibitor to band 3. nonionic detergents and chaps also acted as a nondenaturant in these instances, as well accepted for other proteins. thou ...19854063373
convergence of influences from hypothalamus and locus coeruleus upon the olfactory tubercle.the interaction of locus coeruleus (lc) and lateral hypothalamus (lh) stimulation upon the evoked potentials and unitary activity in the olfactory tubercle (ot) were studied. the difference in latency of the evoked potentials in the ot by lc or by lh stimulation seems to indicate that thin fibers compose the lc-ot pathway and that thick fibers compose the lh-ot pathway. the most common pattern of ot neuronal discharge was in bursts. the train stimulation of the lc decreased the ot neuronal disch ...19854063550
an epidemic of acute diarrhoea in rural southern india associated with echovirus type 11 infection.an epidemic of diarrhoea with two distinct waves affected a village of 1375 people in southern india in 1983. the first wave of the epidemic, from the last week of december 1982, had a sharp peak in january 1983 and was over by march. echovirus type 11 was isolated from patients, who also had a serum antibody response to the virus. during the second wave of the epidemic, from may to september 1983, the clinical features were different and shigella flexneri was isolated without significant viral ...19854067300
fluorometric elisa method for rapid screening of anti-estrogen receptor antibody production in hybridoma cultures.a micro-fluorometric elisa which required only 50 microliter of reagent in each step is reported here for rapid detection of anti-estrogen receptor (er) antibody production by hybridoma cultures. in this study, several factors which influence the sensitivity of elisa, e.g., incubation time, substrates, microtiter plates, concentration of coating antigens, choice and concentration of specific antibody and enzyme were evaluated. we observed that micro-fluorometric elisa using 4-methylumbelliferyl ...19854067317
ledantec virus: identification as a rhabdovirus associated with human infection and formation of a new serogroup.ledantec virus, originally recovered in 1965 from a patient with a febrile illness in senegal, was observed by thin section electron microscopy to be bullet-shaped, representative of members of the rhabdovirus group, with mean dimensions of 164 x 78 nm. particles were observed budding from the plasma membrane and moderate numbers accumulated in the intercellular spaces. in three mammalian cell lines, ledantec virus was rapidly cytopathic and replicated to moderately high titres. in complement fi ...19854067583
classically conditioned hyperglycemia in the obese mouse.the obese (c57bl/6j ob/ob) mouse is a commonly used animal model of non-insulin-dependent diabetes mellitus. it has recently been demonstrated that this mouse is not consistently hyperglycemic, however, unless it is subjected to environmental stress. in the present study, hyperglycemia in obese mice was induced by classical conditioning. obese diabetic mice and lean control animals were exposed to shaking stress. all animals developed hyperglycemia in response to shaking. to demonstrate classica ...19854070525
[experience in the treatment of syphilis with penicillin combined with splenin]. 19854072396
[immune response in splenectomized children to anti-pneumococcal vaccination].the antibody response following anti-pneumococcal immunization with a 14-valent vaccine was studied in 54 children, aged 2 to 18 years after splenectomy. splenectomy had been performed for traumatic, hematologic or immunologic reasons. antibody titration with respect to 12 serotypes was performed by radioimmunoassay immediately before and one month after immunization. changes in responses were studied according to the indication for splenectomy and to the vaccine serotype. the main conclusions o ...19854074086
interdependence of regional expiratory flow.flows from different lung regions interact at the junctions of the bronchial tree, and flow from each region depends on the driving pressures for other regions. at each junction, flow from the region with the higher driving pressure is favored. as a result there is a limit on the difference in alveolar pressures that can develop during expiratory flow from a lung with regional differences in lung compliance and airway resistance. the limiting pressure difference is smaller for lower flow. a nonu ...19854077799
[determination of liver circulation with radionuclides. i. modification of 99mtc-mms accumulation in the liver using drugs].the rate constant of liver uptake of 99mtc-labelled millimicrospheres is taken to be a measure of nutritive res blood supply. animal experiments were used to determine whether this constant--similar to that of other colloids--is affected by drugs such as heparin or corticosteroids which alter res function. in rabbits, the scatter of k between individuals is quite large, with a standard deviation of 27%, whereas the scatter within an individual appears to be acceptable (8%). heparin does not chan ...19854080560
cardiac and systemic hemodynamic actions of the slow channel calcium blocking agent, kb-944.the effects of kb-944 (10-400 micrograms/kg/min, i.v.), a new slow channel calcium blocking agent, on myocardial oxygen utilization, regional myocardial perfusion and hemodynamics were measured in anesthetized dogs. kb-944 produced significant dose-related increases in coronary blood flow and decreases in heart rate, left ventricular systolic pressure, aortic blood pressure and peripheral vascular resistance. at high doses, peak positive and negative dp/dt were both reduced and left ventricular ...19854085780
fourteen years of shigellosis in dhaka: an epidemiological analysis.we examined whether the proportion of shigellae patients among diarrhoeal cases, the distribution, species, case-fatality rates and hospital visits changed over time in dhaka. we isolated 19639 shigella strains from 822812 diarrhoea cases treated at the international centre for diarrhoeal disease research, bangladesh (icddr,b), between 1969 and 1982. the number of cases increased from 209 (2.5%) in 1969 to 4833 (7.7%) in 1976. extrapolating from a 4% vigorous systematic sample of icddr,b hospita ...19854086147
histochemical observations of lipid droplets and glycogen in mouse eggs with abnormal development. 19854087639
alkaline quenching of bacteriorhodopsin tryptophanyl fluorescence: evidence for aqueous accessibility or a hydrogen-bonded chain. 19854089036
alkaline quenching of bacteriorhodopsin tryptophanyl fluorescence: evidence for aqueous accessibility or a hydrogen-bonded chain. 19854089036
[ultrastructural aspects of the wound healing process of the skin of albino rats]. 19854089424
muscle cross-bridge kinetics in rigor and in the presence of atp analogues.recently we reported preliminary mechanical experiments on freshly skinned rabbit psoas fibers that suggested that while almost all of the cross-bridges are attached to actin in the presence of 4 mm adenyl-5'-yl-imidodiphosphate (amp-pnp) (ionic strength, 0.13 m), there is an equilibrium between the attached and detached states, so that, in the presence of 4 mm amp-pnp, fibers should not be able to maintain tension (schoenberg, et al., 1984, in contractile mechanisms in muscle, pollack and sugi, ...19854092069
[immunochemical analysis of oligosaccharide fragments of o-specific shigella flexneri polysaccharides].comparison of inhibitory properties of several synthetic oligosaccharides related to sh. flexneri o-specific polysaccharides, namely glc alpha 1-3rha alpha 1-ome, glc alpha 1-3rha alpha 1-2rha alpha 1-ome, rha alpha 1-2(glc alpha 1-3)rha alpha 1-ome, glcnac beta 1-2(glc alpha 1-3)rha alpha 1-ome, and glcnac beta 1-2(glc alpha 1-3) rha alpha 1-2rha alpha 1-ome, using passive haemagglutination reaction demonstrated the tetrasaccharide to possess the highest activity in v; 7,8-anti-7,8 immune syste ...19846083795
glycocalyx visualization in shigella flexneri. 19846085609
[in vitro activity of a new cephalosporin (ceftriaxone) on recently isolated strains of shigella]. 19846088342
virulence of entamoeba histolytica trophozoites. effects of bacteria, microaerobic conditions, and metronidazole.the association of axenically grown trophozoites of entamoeba histolytica strains hk-9 or hm-1:imss with various types of gram-negative bacteria for relatively short periods markedly increased their virulence, as evidenced by their ability to destroy monolayers of tissue-cultured cells. interaction of trophozoites with bacteria that were heat inactivated, glutaraldehyde fixed, or disrupted by sonication, or bacteria treated with inhibitors of protein synthesis, did not augment amebic virulence. ...19846088660
cloning and characterization of reca genes from proteus vulgaris, erwinia carotovora, shigella flexneri, and escherichia coli b/r.the reca genes of proteus vulgaris, erwinia carotovora, shigella flexneri and escherichia coli b/r have been isolated and introduced into escherichia coli k-12. all the heterologous genes restore resistance to killing by uv irradiation and the mutagen 4-nitroquinoline-1-oxide in reca- e. coli k-12 hosts. recombination proficiency is also restored as measured by formation of lac+ recombinants from duplicated mutant lacz genes and the ability to propagate phage lambda derivatives requiring host re ...19846090408
aerobactin genes in shigella spp.aerobactin, a hydroxamate iron transport compound, is synthesized by some, but not all, shigella species. conjugation and hybridization studies indicated that the genes for the synthesis and transport of aerobactin are linked and are found on the chromosome of shigella flexneri, s. boydii, and s. sonnei. the genes were not found in s. dysenteriae. a number of aerobactin synthesis mutants and transport mutants have been isolated. the most common mutations are deletions of the biosynthesis or bios ...19846090419
mercuric ion-resistance operons of plasmid r100 and transposon tn501: the beginning of the operon including the regulatory region and the first two structural genes.the mercuric ion-resistance operons of plasmid r100 (originally from shigella) and transposon tn501 (originally from a plasmid isolated in pseudomonas) have been compared by dna sequence analysis. the sequences for the first 1340 base pairs of tn501 are given with the best alignment with the comparable 1319 base pairs of r100. the homology between the two sequences starts at base 58 after the end of the insertion sequence is-1 of r100. the sequences include the transcriptional regulatory region, ...19846091128
evidence for clustering of rna polymerase and ribosomal protein genes in six species of enterobacteria.32p-labelled dna fragments from the e. coli beta operon were used as hybridisation probes for homologous dna sequences in chromosomal digests of six species of enterobacteria. in all species the hybridisation pattern suggested clustering of the genes comprising the beta operon.19846092848
peyer's patch lymphoid follicle epithelial adherence of a rabbit enteropathogenic escherichia coli (strain rdec-1). role of plasmid-mediated pili in initial adherence.escherichia coli (strain rdec-1) adheres to m cells of rabbit peyer's patch lymphoid follicle epithelium. the rdec-1 strain contains an 85 x 10(6) d plasmid that codes for pili, which, when purified, adhere to gut absorptive epithelium. this study compared the in vivo lymphoid follicle adherence of the rdec-1 strain with that of a shigella flexneri (shd15) that contained the 85 x 10(6) d plasmid and expressed the rdec-1 pili, a control e. coli, and a control s. flexneri (shd12). the bacteria wer ...19846145724
[the conjugation system determined by plasmid r477 and other plasmids belonging to the h2 incompatibility group]. 19846148780
the shigella flexneri o-antigenic polysaccharide chain. nature of the biological repeating unit.the sequence of monosaccharides in the biological repeating tetrasaccharide unit of shigella flexneri variant y o-antigenic polysaccharide chain was determined by subjecting three oligosaccharides of the polysaccharide, obtained by phage-sf6-mediated enzymatic hydrolysis, to methylation analysis and proton nuclear magnetic resonance spectroscopy. the smallest saccharide was shown to be a tetrasaccharide with the structure alpha-l-rhap-(1-2)-l-rha. the next saccharide, an octasaccharide, was show ...19846199198
[clinico-diagnostic significance of specific antigenemia in acute dysentery]. 19846205453
[preventive effectiveness of dried polyvalent shigella bacteriophage in organized collective farms]. 19846235671
bacteriophage mu d1(apr lac) generates vir-lac operon fusions in shigella flexneri 2a.previous studies have demonstrated that expression of virulence in shigella spp. is controlled by growth temperature. to study the regulation of virulence (vir) genes, we set out to develop a rapid, easily-assayed phenotype with which to measure expression of virulence. this report described a procedure for isolating vir-lac operon fusions in s. flexneri 2a by using the specialized transducing bacteriophage mu d1(apr lac) of casadaban and cohen (m. casadaban and s. n. cohen, proc. natl. acad. sc ...19846236150
entamoeba histolytica: virulence enhancement of isoenzyme-stable parasites.pathogenic entamoeba histolytica isolated from patients with clinical amoebiasis can be differentiated from nonpathogenic e. histolytica obtained from asymptomatic carriers on the basis of the electrophoretic pattern of their isoenzymes. virulence of different strains of axenically grown trophozoites of entamoeba histolytica, as determined by various laboratory tests, such as damage to tissue culture monolayers, or their ability to cause an hepatic abscess in a hamster, are known to vary conside ...19846325226
comparative efficacies of pivmecillinam and ampicillin in acute shigellosis.the clinical efficacies of pivmecillinam and ampicillin were compared in a randomized double-blind trial in the treatment of acute shigellosis. of 44 adult male patients, all culture positive for shigella strains, 22 patients received 400 mg of pivmecillinam and 22 patients received 500 mg of ampicillin every 6 h. both drugs were administered orally for 5 days. four patients receiving ampicillin were infected with shigella strains that were resistant to ampicillin but susceptible to pivmecillina ...19846329092
changes in the trend of shigellosis in dhaka: family study on secondary infection, clinical manifestation and sensitivity pattern: 1980.the incidence of shigellosis and the death rate have increased and the resistance of shigellae to antibiotics has changed in dhaka during our experiences. in 1980, we investigated the secondary infection and case rates, infection to case ratio, duration of illness, excretion of shigellae and antibiotic sensitivity pattern in 100 families with cases of shigellosis, culturing rectal swabs obtained by home visits for a 10-day period. standard methods were used for culture and sensitivity tests. the ...19846380011
[structure of a shigella flexneri population in the process of forming microcolonies].the study of the adaptation period of the s- and r-forms of s. flexneri in a nutritionally sufficient medium has revealed that the population structure characteristic of the s-form and ra-chemotype comprises two classes of cells: actively growing and nonviable. besides these two classes, the class of spontaneously lysing bacteria is present in the population of mutants with various degrees of lipopolysaccharide (lps) lesions. the number of bacteria subject to autolysis increases with the progres ...19846380164
purification and properties of carboxylesterase b of escherichia coli.carboxylesterase b produced by escherichia coli was purified 1,350-fold with a recovery of 12% by successive gel filtrations, deae-trisacryl, phenyl-sepharose chromatography and preparative electrophoresis. the purified enzyme was found to be homogeneous, as judged by a single precipitation line in ouchterlony double diffusion in an experiment with homologous antiserum. the apparent molecular weight determined by gel filtration and the isoelectric point determined by electrofocusing were 57,000 ...19846380369
cytotoxicity of human peripheral blood and colostral leukocytes against shigella species.we examined the ability of human peripheral blood leukocytes to kill strains of shigella sonnei and shigella flexneri by using a modified bactericidal assay. antibody-dependent cellular cytotoxicity (adcc) was demonstrated in the presence of specific rabbit immune serum directed against s. sonnei. with peripheral blood leukocytes from adults, adcc was found only in the mononuclear cell and purified lymphocyte populations. monocyte-macrophages and polymorphonuclear leukocytes were unable to demon ...19846384045
heterogeneity of lipid a: comparison of lipid a types from different gram-negative bacteria.chloroform-soluble purified lipid a preparations from 10 sources, including five escherichia coli strains (eh100, k-12, o127, o111, rcdc), two salmonella strains (salmonella typhimurium, salmonella minnesota r595), shigella sonnei ii, and a hybrid of shigella flexneri and e. coli k-12, were compared with lipid a from s. flexneri. purified lipid a from s. flexneri was earlier found to be composed of eight fractions. the various lipid a preparations were assayed by thin-layer chromatography. chrom ...19846384185
effect of cytochalasin b and dihydrocytochalasin b on invasiveness of entero-invasive bacteria in hep-2 cell cultures.the effect of cytochalasin b (cb) and di-hydrocytochalasin b (h2cb) on the invasiveness of salmonella typhimurium, shigella flexneri and yersinia enterocolotica serotype 0:3 in hep-2 cell cultures was examined. the intra-cellular and extra-cellular bacteria were identified by a combination of nomarski interference contrast microscopy and uv incident light microscopy applied on the same light microscope. pre-treatment of cells with cb and h2cb inhibited the uptake of s. typhimurium and s. flexner ...19846385614
usefulness of plasmid profiles for differentiation of shigella isolates in bangladesh.we studied the plasmid profiles of 136 shigella isolates in bangladesh to determine whether plasmid profiles could be used for differentiation of strains for epidemiological studies. many different plasmid patterns were observed within each species, indicating that many genetically different strains of shigella are responsible for illness in bangladesh.19846386850
oral vaccination of monkeys with an invasive escherichia coli k-12 hybrid expressing shigella flexneri 2a somatic antigen.a living oral vaccine, designed to protect against shigella flexneri 2a infections, was constructed by using escherichia coli k-12 as a carrier strain. the hybrid strain, designated ec104, contained both chromosomal and plasmid genes from s. flexneri donor strains. in addition to expressing the s. flexneri 2a somatic antigen, it had inherited the property of epithelial-cell invasion. after the oral administration to rhesus monkeys, ec104 was isolated from the feces for up to 3 days, but by day 4 ...19846389344
the prevalence of gastro-intestinal parasites and bacteria in the game scouts at the kainji lake national park of nigeria.the faeces of twenty scouts working at borgu games were examined for the presence of parasites and bacteria. cysts of entamoeba histolytica, giardia lamblia and trichomonas hominis were found. helminth ova encountered included those of ancylostoma duodenale, ascaris lumbricoides, stronglyloides stercoralis and trichuris trichiura. escherichia coli and shigella flexneri were isolated from one faecal sample each. in all, parasites were found in the faeces of only 9 scouts. the epidemiological sign ...19846389403
[bacillary dysentery 1982]. 19846390523
[clinical and treatment characteristics of acute dysentery caused by shigella boydii and newcastle]. 19846390958
[further decrease in intestinal infection morbidity in the ukrainian ssr]. 19846390962
[conditions and factors determining the etiological structure of dysentery and its alterations. ii. concepts, hypotheses and points of view yesterday and today]. 19846391040
[conditions and factors determining the etiological structure of dysentery and its alterations. iii. the epidemiological assessment of the biological properties of shigella and their importance in the development of the epidemic process in different nosological forms of dysentery]. 19846391041
dna hybridization technique to detect shigella species and enteroinvasive escherichia coli.a dna hybridization method for the detection of shigella species and enteroinvasive escherichia coli is described. it is based upon the high degree of homology shared by the virulence plasmids present in all pathogenic strains. after 32p labeling, a 17-kilobase ecori restriction fragment of a virulence plasmid belonging to shigella flexneri serotype 5 was used as a probe to detect shigella species and enteroinvasive e. coli in isolated colonies. this method proved highly specific as well as sens ...19846392327
shigella vulvovaginitis in a three-year-old child: a case report. 19846392838
[identification of specific antibodies in dysentery]. 19846393359
shigellemia. 19846394497
association of pneumonia with undernutrition and shigellosis. 19846394498
the effect of postnatal age on the adherence of shigella flexneri, escherichia coli 0124, and e. coli 0128 to guinea pig intestinal cells.since postnatal development of the gastrointestinal tract has an important effect on its microbial flora and may influence the types of intestinal infections, we examined the effect of age on bacterial adherence to intestinal epithelial cells. radiolabeled bacteria were incubated with guinea pig enterocytes released by treating loops of the intestine with solutions containing edta, dithiothreitol, and citrate. nonbound bacteria were separated from intestinal cells by sedimentation on a percoll g ...19846395082
[species of shigella isolated in a 4-year period and antimicrobial sensitivity]. 19846395307
[structure of the causative agents of shigellosis in areas of the ussr 1979-1981].summarized data on the specific structure of shigella strains isolated in different territories of the ussr in 1979-1981 are presented. these data indicate that in the intra-specific structure of s. flexneri strains the proportion of serological subvariant 2a increased and that of serological variant 6 and subvariants 16 and 4a decreased during this period. using one of the base territories of the all-union shigellosis center as an example, the spread of an unusual serological variant of s. flex ...19846395590
[cathepsin d activity in splenocytes in relation to shigella virulence in an experiment].the overtime study of changes in the activity of cathepsin d, a lysosomal enzyme, in the splenocytes of cba mice after their infection with virulent and avirulent shigella strains of the same origin and with the same antigenic structure has been made. as the result of two months of observations, changes in the activity of this enzyme in the cytoplasmic and lysosomal cell fractions have been found to occur in phases. the activity of cathepsin d has been shown to depend on the virulence of shigell ...19846395592
shigellosis: an old disease with new faces. 19846397507
can erythema nodosum and reactive arthritis be a sequel to shigella flexneri gastroenteritis?erythema nodosum and seronegative arthritis associated with diarrhoea was previously regarded as implying the presence of inflammatory bowel disease. recently, however, erythema nodosum has been described as a sequel to diarrhoea caused by infection with salmonella typhimurium, yersinia enterocolitica and campylobacter jejuni. we report two patients in whom erythema nodosum and reactive arthritis followed acute shigella flexneri gastroenteritis, a rare association.19846398514
protective properties of peptidoglycan associated protein of shigella flexneri.groups of ch3/hej mice were immunized with peptidoglycan associated protein (pgp) from shigella flexneri 3a administered in a single intraperitoneal injection (5 micrograms per mouse). after various periods post immunization the animals were challenged intraperitoneally with homologous bacteria. pgp proved to be strongly immunogenic. as early as one week after immunization all the animals survived challenge with 100 x ld100, and one third of the animals survived challenge with a dose of 500 x ld ...19846398687
changing patterns of drug resistance in shigella flexneri serotypes (1978-82). 19846399026
vaccine strain sh. flexneri t32-istrati. studies in animals and in volunteers. antidysentery immunoprophylaxis and immunotherapy by live vaccine vadizen (sh. flexneri t32-istrati). 19846400299
determination of humoral antibodies against shigella flexneri 2a t32 (istrati) by elisa. 19846400300
study on the effect of oral immunization of t32-istrati strain against bacillary dysentery in field trials. 19846400301
immunoprophylaxis against bacillary dysentery by dysentery live vaccine vadizen (shigella flexneri t32-istrati) in children collectivities in sv county. 19846400302
results of dysentery vaccinations by live vaccine vadizen (sh. flexneri 2a t32-istrati) in children collectivities in gl. 19846400303
immunoprophylactic effect of dysentery vaccination by live dysentery vaccine vadizen (sh. flexneri t32-istrati) in children and adult collectivities in bt county. 19846400304
antidysentery vaccination by live vaccine vadizen (sh. flexneri t32-istrati) in children collectivities in ph county. 19846400305
investigations concerning the immunoprophylactic effect of the antidysentery vaccination by live vaccine vadizen (sh. flexneri t32-istrati) in children. 19846400306
prophylaxis of bacillary dysentery by liver vaccine vadizen (sh. flexneri t32-istrati) in children collectivities in is county. 19846400307
economical evaluation of the dysentery vaccination in b municipality. 19846400308
aspects of the dysentery evolution in the territory surveilled by the sanitary-antiepidemic center of b municipality--laboratory no 2 (administrative district 2 and 3) after the introduction of the dysentery vaccination. 19846400309
reduction of morbidity due to bacillary dysentery by live vaccine vadizen (sh. flexneri t32-istrati) in children collectivities in bh county. 19846400310
improvement of production and control methods of live dysentery vaccine vadizen (shigella flexneri 2a t32-istrati). 19846400311
acute bacterial diarrhoeas due to salmonella, shigella and enteropathogenic escherichia coli in zambia. 19846400676
[a possible way of improving colibacterin]. 19846401093
[a comparative analysis of beta-lactamase activity in enterobacteria]. 19846401094
temperature-dependent expression of virulence genes in shigella species.the pathogenicity of shigella spp. involves the ability of the bacteria to penetrate and replicate within the epithelial cells of the large intestine. model systems for examining the virulence of shigellae employ henle intestinal epithelial cells in tissue culture and an in vivo assay for virulence in guinea pig eyes (sereny test). using these systems, we studied the genetic and physiological bases for the ability of shigellae to invade epithelial cells. we found that expression of virulence in ...19846360895
loss of pigmentation in shigella flexneri 2a is correlated with loss of virulence and virulence-associated plasmid.in this study, we examined the relationship between the virulence of shigella flexneri 2a and the ability of strains of s. flexneri 2a to absorb congo red. spontaneous nonpigmented (i.e., unable to bind congo red [pcr-]) derivatives of a virulent, pigmented (pcr+) strain of s. flexneri 2a were isolated and assayed for virulence as determined by their ability to invade epithelial cells. all pcr- mutants examined lost the ability to invade epithelial cells and were thus avirulent. agarose gel elec ...19846360906
splenic abscess caused by shigella flexneri and bacteroides fragilis.we report a case of splenic abscess from which only shigella flexneri (serotype 1a) and bacteroides fragilis were isolated. the patient was a 59-year-old diabetic female who displayed minimal gastrointestinal symptoms. the s. flexneri isolate was resistant to chloramphenicol and tetracycline. the possible synergistic relationship between the two organisms is discussed.19846361064
lipid a fractions analyzed by a technique involving thin-layer chromatography and enzyme-linked immunosorbent assay.a modified enzyme-linked immunosorbent assay (elisa), with alkaline phosphatase as enzyme, was used for the study of antigenicity of lipid a fractions directly on thin-layer chromatographic (tlc) plates. for visualization a gel slab containing the enzyme substrate was placed on the plate containing enzyme-conjugated antibodies. the plate was read by a thin-layer chromatogram spectrophotometer. the immunoassay was both highly specific and quite sensitive. sensitivity was superior to levels obtain ...19846365543
[food infection caused by shrimp]. 19846366590
[chromosome markers of drug resistance as the selectors of potential vaccinal strains].mutations resulting in acquisition of the resistance to rifampicin, streptomycin, neamine, polymyxin m, mecillinam and high concentrations of nalidixic acid were studied with respect to their effect on virulence of shigella flexneri by transduction of the markers from the spontaneous avirulent mutants to the initial strain. it was shown that the acquisition of the determinants of the resistance to rifampicin, streptomycin, neamine (nea a) and high concentration of nalidixic acid resulted in 100 ...19846367629
[transfer of resistance in shigellae to azlocillin and other antibiotics]. 19846367893
r plasmids conferring multiple drug resistance from shigella isolated in korea.the majority (85%) of shigella isolated in 1980 and 1981 in korea were shigella flexneri, the others were sh. sonnei (14%) with only a small number of sh. dysenteriae. only 14 of the 459 strains of shigella isolated were susceptible to all 12 drugs tested, and 445 were resistant to three or more drugs. strains multiply resistant to the six drugs, chloramphenicol (cm), tetracycline (tc), streptomycin (sm), sulfisomidine (su), ampicillin (ap) and trimethoprim (tp) were most frequently encountered, ...19846368684
[further research on the use of media with na-rna for isolating shigella].the isolation of shigellae from the feces of patients with the use of ploskirev's bactoagar with na-rna added at a concentration of 4 mg/ml has facilitated the laboratory diagnosis of dysentery due to the increased resolution of the medium. the isolation of shigellae on the medium with the stimulating agent added occurs 9% more frequently than on the control medium.19846369838
shigella dysentery in tripoli, libya. 19846371260
leads from the mmwr. shigellosis among tourists--union of soviet socialist republics, 1983. 19846371268
[bacillary dysentery (shigella flexneri type 2) caused by shrimps]. 19846371557
[basic approach to the production of potential vaccine strains of salmonella, shigella and pasteurella, capable of reproduction, using suitable genetic markers]. 19846375171
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