Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| mercuric reductase structural genes from plasmid r100 and transposon tn501: functional domains of the enzyme. | the nucleotide sequence for the 2240 bp of plasmid r100 following the merc gene of the mercuric resistance operon has been determined and compared with the homologous sequence of transposon tn501. the sequences following merc and preceding the next structural gene mera are unrelated between r100 and tn501 and differ in length, with 72 bp in tn501 and 509 bp in r100. the r100 sequence has a potential open reading frame (orf) for a 140 amino acid polypeptide with a reasonable translational start s ... | 1985 | 2989109 |
| cloning of plasmid dna sequences involved in invasion of hela cells by shigella flexneri. | a large plasmid is found in virulent isolates of shigella sp. and encodes functions essential for invasion of mammalian cells. to identify plasmid sequences necessary for invasion, we isolated a series of tn5 insertions in pwr100, the virulence plasmid of shigella flexneri serotype 5. these insertions demonstrated that three separate ecori fragments of pwr100 were required for invasion of hela cells. however, the corresponding native ecori fragments, when cloned into pbr325, did not restore viru ... | 1985 | 2989179 |
| inhibition and activation of bovine heart nad-specific isocitrate dehydrogenase by atp. | in the absence of added calcium, inhibition of nad-specific isocitrate dehydrogenase by atp occurred without adp (i0.5 = 1.8 mm) and with 0.2 mm adp3- (i0.5 = 1.0 mm) at subsaturating substrate concentrations at ph 7.4. inhibition by atp was competitive with nad+ in the presence and absence of adp and was not reversed by magnesium citrate. no reversal of atp inhibition by free ca2+ was observed in the presence of adp (0.2 mm). however, when adp was absent, increasing ca2+ first caused progressiv ... | 1985 | 2990340 |
| bowenoid papulosis. presence of human papillomavirus (hpv) structural antigens and of hpv 16-related dna sequences. | this study reviews 39 cases of anogenital bowenoid papulosis lesions in 22 individuals of both sexes that were analyzed clinically, histologically, immunocytochemically, and virologically. macroscopically, three different types of lesions were demonstrated: erythematous macules; papules (lichenoid and/or pigmented papules); and leukoplakialike lesions. microscopically, bowenoid papulosis fulfills the criteria of a squamous cell carcinoma in situ. much like oral precancers, three distinct growth ... | 1985 | 2990353 |
| [survival of aujeszky's disease virus in infected pig slurry]. | it has been demonstrated that after experimental infection of pig slurry from the space under the slatted floor (infection dose of 10(6)pfu per ml), the aujeszky's disease virus (adv) survived for 72 hours at the temperature of 15 degrees c and at ph 6.5, but was inactivated after 96 hours. when technologically treated pig slurry from the storage tanks was saturated with water and infected with adv at the dose of 10(5)pfu per ml, the virus survived for 23 days when kept at 15 degrees c and 4 deg ... | 1985 | 2992149 |
| rates of cholesterol synthesis and low-density lipoprotein uptake in the adrenal glands of the rat, hamster and rabbit in vivo. | the absolute rate of cholesterol acquisition from de novo synthesis and from receptor-dependent and receptor-independent low-density lipoprotein (ldl) uptake was determined in the adrenal glands of the rat, hamster and rabbit under in vivo conditions. the rate of incorporation of [3h]water into cholesterol in the adrenal gland was much higher in the hamster (1727 nmol/h per g) and rabbit (853 nmol/h per g) than in the rat (71 nmol/h per g). assuming that 23 atoms of 3h are incorporated into the ... | 1985 | 2992599 |
| suicide vector for transposon mutagenesis in pseudomonas solanacearum. | a suicide vector was constructed by cloning the transfer genes of the wide-host-range (incw group) plasmid r388 into the bamhi site of pbr325. this plasmid can deliver tn5 into pseudomonas solanacearum at frequencies ranging from 10(-6) to 10(-9) per recipient. | 1985 | 2993241 |
| amino acid sequence of acylphosphatase from porcine skeletal muscle. | the amino acid sequence of acylphosphatase from porcine skeletal muscle was determined. it consists of 98 amino acid residues with n-acetylserine at the amino (n)-terminus: ac-ser-thr-ala-arg-pro-leu-lys-ser-val-asp-tyr-glu-val-phe-gly -arg-val-gln-gly-val-cys-phe-arg-met-tyr-thr-glu-asp-glu-ala-arg-lys-ile -gly-val-val-gly-trp-val-lys-asn-thr-ser-lys-gly-thr-val-thr-gly-gln -val-gln-gly-pro-glu-glu-lys-val-asn-ser-met-lys-ser-trp-leu-ser-lys -ile-gly-ser-pro-ser-ser-arg-ile-asp-arg-thr-asn-phe- ... | 1985 | 2993259 |
| the biologic significance of the mixed lymphocyte kidney culture in humans. | the mixed lymphocyte kidney culture (mlkc) in humans has been studied in normal and abnormal clinical conditions. human renal cortical cells were extracted by collagenase treatment from the kidneys of "normal" heart-beating cadaver organ donors (n = 13), patients with end-stage renal disease (esrd) at pretransplant bilateral nephrectomy and splenectomy (n = 13), and from irreversibly rejected renal allografts at the time of graft nephrectomy (n = 5). proliferation of peripheral blood t lymphocyt ... | 1985 | 2996186 |
| [the virus causing human t-cell lymphomas-leukemias]. | 1985 | 2996236 | |
| the e7-associated cell-surface antigen: a marker for the 11p13 chromosomal deletion associated with aniridia-wilms tumor. | unbalanced interstitial deletions of the p13 region of human chromosome 11 have been associated with congenital hypoplasia or aplasia of the iris, mental retardation, ambiguous genitalia, and predisposition to wilms tumor of the kidney. utilizing somatic cell hybrids containing either the normal or abnormal chromosome 11 from a child with wilms tumor and aniridia, we previously mapped the e7 cell-surface antigen to the 11p1300-to-11p15.1 region. to localize even further the site of this antigen ... | 1985 | 2996335 |
| acquired immune deficiency syndrome. a perspective for the medical practitioner. | aids is a new disease process with complications and management problems unlike anything ever seen before. an attempt has been made to summarize the available information about its various clinical presentations and how to manage them. subjects covered will include associated malignancies, infections, transmissibility, prodromal states, and infection control issues. | 1985 | 2997554 |
| pyrimidinones. 1. 2-amino-5-halo-6-aryl-4(3h)-pyrimidinones. interferon-inducing antiviral agents. | interferon induction and antiviral activity was discovered with 2-amino-5-bromo-6-phenyl-4(3h)-pyrimidinone. an analogue study incorporating a series of 2-amino-5-substituted-6-arylpyrimidinones revealed that the most potent interferon inducers were mono- and difluorophenyl analogues. these same analogues were also potent antiviral agents against semliki forest virus and herpes simplex type 1. in addition the monomethoxyphenyl analogues were potent antiviral agents but weak interferon inducers. ... | 1985 | 2999405 |
| elicitation of conspecific attack or defense in the male rat by intraventricular injection of a gaba agonist or antagonist. | the involvement of central gabaergic mechanisms in the control over offensive and defensive behaviours in the rat was studied using intracerebroventricular injections (5 microliter) of a gaba agonist (thip) or a gaba antagonist (bicuculline methiodide). intracerebroventricular injections of thip (1.25 and 2.5 micrograms) induced attacks and offensive sideways towards an untreated partner, in animals placed in a neutral area where no aggressive reactions occur in controls. social approach behavio ... | 1985 | 2999844 |
| elicitation of conspecific attack or defense in the male rat by intraventricular injection of a gaba agonist or antagonist. | the involvement of central gabaergic mechanisms in the control over offensive and defensive behaviours in the rat was studied using intracerebroventricular injections (5 microliter) of a gaba agonist (thip) or a gaba antagonist (bicuculline methiodide). intracerebroventricular injections of thip (1.25 and 2.5 micrograms) induced attacks and offensive sideways towards an untreated partner, in animals placed in a neutral area where no aggressive reactions occur in controls. social approach behavio ... | 1985 | 2999844 |
| [effects of cycleanine dimethobromide on heart conduction system]. | 1985 | 2945367 | |
| human t-cell leukemia/lymphoma viruses: atl and aids. | 1985 | 2897327 | |
| measurement of igg in equine blood by immunoturbidimetry and latex agglutination. | this paper describes the quantitative measurement of igg concentrations in equine blood/serum by turbidimetric analysis and the qualitative assessment using latex agglutination. the concentration of igg in foal serum measured by turbidimetry correlated well with the results obtained by radial immunodiffusion (r = 0.91) and the gamma-globulins estimated from the electrophoresis of serum on cellulose acetate (r = 0.92). the method, using antibody-coated latex beads, to detect foals with serum igg ... | 1985 | 2580703 |
| structural analysis of the o-glycosidically linked core-region oligosaccharides of human meconium glycoproteins which express oncofoetal antigens. | glycoproteins were extracted from meconium samples of group o neonates of secretor type by pronase digestion followed by precipitation in 67% aqueous ethanol and separated into ii antigen enriched and depleted fractions by affinity chromatography. the latter fraction strongly expressed the oncofoetal antigens recognised by natural antibodies in mouse sera and the hybridoma antibody fc 10.2, and this activity was enhanced after mild acid hydrolysis to remove sialic acid and fucose residues. oligo ... | 1985 | 2580706 |
| the effects of subdiabetogenic streptozotocin doses on rat beta cell volume and functions. | the influence of a single i.v. injection of 30 mg/kg streptozotocin (sz) into female wistar rats on plasma glucose, pancreatic insulin content, volume and function of the remaining beta-cells has been investigated. 14 days after sz treatment most animals remained normoglycemic; only about 15% showed permanent hyperglycemia. the pancreatic insulin content in normoglycemic rats was reduced to 30%, whereas in hyperglycemic rats only 6% of control values could be detected. the remaining beta-cell vo ... | 1985 | 2581562 |
| [synthesis of oligosaccharide fragments of o-specific shigella flexneri polysaccharides. ii. synthesis of trisaccharide glc alpha1----3rha alpha1----2rha alpha1----ome and tetrasaccharide glcnac beta1----2(llc alpha1----3)rha alpha1----2rh alpha1----ome]. | methyl glycosides of the title linear trisaccharide and branched tetrasaccharide were synthesized by stepwise glycosylation. these oligosaccharides represent the fragments of o-antigenic polysaccharides of shigella flexneri serotypes 2b, 3a, 5b, and x. | 1985 | 2581587 |
| inflammatory bowel disease. | the place of inflammatory bowel disorders in the spondarthritis matrix has been discussed and aetiopathological and clinicoradiological features of their individual arthropathies described. particular emphasis has been placed on the arthropathies of ulcerative colitis and crohn's disease, but a comment is also included on the much rarer condition, whipple's disease. the position of reactive arthritis resulting from enteric infection in relation to the spondarthritis concept is examined in the li ... | 1985 | 2581732 |
| [theoretical conformation analysis of tetrasaccharide-repeated links of the shigella flexneri o-antigenic polysaccharide]. | theoretical conformational analysis of four tetrasaccharide repeating units of the shigella flexneri serogroup y polysaccharide has been carried out. interdependency of conformational states of neighbouring disaccharide units in the oligosaccharides has been investigated and conformational distribution of tetrasaccharides in solution calculated. taking into account the entropy of oligosaccharide chains is shown to lead to significant correction of the results. | 1985 | 2408628 |
| a heat-modifiable outer membrane protein carries an antigen specific for the species yersinia enterocolitica and yersinia pseudotuberculosis. | the outer membranes of gram-negative bacteria are considered to be of importance in host-bacteria interaction, in protective immunity, and occasionally in subclassification within a species. in this study, the outer membranes of several strains of yersinia enterocolitica and y. pseudotuberculosis were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page). it was found that the appearance of the major proteins depended on the temperature at which they were solubilized i ... | 1985 | 2409152 |
| [synthesis of oligosaccharide fragments of o-specific polysaccharides of shigella flexneri. iii. synthesis of the tetrasaccharide glc alpha 1-3rha alpha 1-2(glc alpha 1-3)rha alpha 1-ome and the pentasaccharide glcnac beta 1-2(glc alpha 1-3)rha alpha 1-2(glc alpha 1-3)rha alpha 1-ome]. | two synthetic schemes to prepare the title branched tetrasaccharide and pentasaccharide are described. these oligosaccharides represent fragments of the o-antigenic polysaccharides of shigella flexneri serotype 5b. | 1985 | 2412567 |
| autoaggressive t lymphocyte lines recognizing the encephalitogenic region of myelin basic protein: in vitro selection from unprimed rat t lymphocyte populations. | autoimmune t lymphocyte lines have been established from unprimed normal rat lymph node cell populations. in a first, negative-selection round, spontaneously proliferating (smlr) t cells were eliminated by a pulse of budr followed by short wave light irradiation. in a second, positive-selection round, the smlr-depleted populations were confronted with mbp presented by syngeneic spleen adherent cells. reactive t cells were propagated until stable, permanent t lines were established. all lines wer ... | 1985 | 2413113 |
| [membrane activity of a cell-free filtrate of shigella flexneri 2a]. | in the course of metabolism in shigella flexneri 2a strain there was noted secretion of a membrane-tropic factor into media. this factor increased the conductivity of bilayer lipid membranes causing formation of ionic channels in them. minimal conductivity of the channels was about 25 ps in 0.5 m nacl; at ph 7-8 the channels exhibited cation-anion selectivity. the calculated number of cation transfer (t+) was elevated with an increase in the channel amplitude, approaching 0.86. since the conduct ... | 1985 | 2413620 |
| [use of immunoenzyme analysis for evaluating the specificity of the antibody recognition of the antigenic determinants of streptococcus group a]. | investigations carried out with the use of a celia system have revealed that antibodies in the sera of patients with primary erysipelas and antibodies in rabbit antiserum to the ribosomes of group a streptococcus specifically bind with adsorbed streptococcal ribosomes, recognizing the antigenic determinants of streptococcal ribosomes, which differ from those of individual gram-negative prokaryotes (escherichia coli, shigella sonnei, shigella flexneri, salmonella minnesota). the modified celia sy ... | 1985 | 2413662 |
| [immune response and immune tolerance to the o-antigen of shigella flexneri vi]. | the method for the determination of the number of cells synthetizing antibodies to s. flexneri vi o-antigen in the spleen of mice has been developed. primary immune response to this antigen has been studied with the use of the new method. immune response to the optimum immunogenic dose of o-antigen has a manifest variable character. the intensity of primary immune response has been shown to rise with the increase of the dose of o-antigen from 0.004 to 50 micrograms. the preliminary injection of ... | 1985 | 2413664 |
| genetic determinants of virulence in shigella and dysenteric strains of escherichia coli: their involvement in the pathogenesis of dysentery. | 1985 | 2414072 | |
| [specific antigens of the causative agents and their antibodies in the circulating immune complexes in acute dysentery]. | the level of circulating immune complexes has been determined in 53 patients in the dynamics of the disease. for the first time circulating immune complexes have been found to contain shigella sonnei k-antigen and shigella flexneri o-antigen, as well as iga, igg and igm to shigella. shigella antigens can be detected from the first week of the disease, and their occurrence does not depend on the level of circulating complexes in patients blood serum. | 1985 | 2414952 |
| [effect of vitamins of the b group on the accumulation of complex antigens of dysentery bacteria in the process of deep cultivation]. | 1985 | 2416151 | |
| [comparative characteristics of antigenic conversion induced by shigella flexneri and escherichia coli 0129 phages]. | the interaction of s. flexneri converting phages pe5, p90 and fv with e. coli antigenic variant o129, e. coli o129 converting phage vb with the above antigenic variant and with s. flexneri y-variant was studied. phage pe5 and phage vb were found to induce the conversion of o-antigen in e. coli antigenic variant 0129 and in s. flexneri y-variant with the detection of antigens v and 7,8. phages p90 and fv induced no conversion of o-antigen. changes in the antigenic properties of convertants were c ... | 1985 | 2416154 |
| striatal synaptosomal dopamine synthesis: evidence against direct regulation by an autoreceptor mechanism. | regulation of the rate-limiting step in dopamine (da) synthesis was estimated in striatal synaptosomes by measuring the rate of hydroxylation of l-4-[3h]phenylalanine, a substrate of tyrosine hydroxylase (th). da inhibited hydroxylation with an ic50 of 0.2 microm. the concentration-response curve of da-induced inhibition was not affected by the presence of 1 microm chlorpromazine, a phenothiazine da antagonist. sulpiride and haloperidol, da antagonists of the benzamide and butyrophenone classes ... | 1985 | 2859213 |
| prolactin and its involvement in fluid regulation in the bovine. | plasma prolactin (prl) concentrations, osmolality, water consumption, feed intake, urine excretion, and fecal water output were determined in twelve steers of 3 breeds exposed to 5 feed and water regimes. breed differences were found in water intake and plasma prl concentrations when feed and water were ad lib., however, during any of the other 4 treatments, responses were similar between breeds. during dehydration and feed restriction, water intake, urine, fecal water, and plasma prolactin decr ... | 1985 | 2859961 |
| effects of excitatory amino acids on calcium transport by brain membranes. | the effect of excitatory amino acids on the uptake of 45ca was studied in crude mitochondrial (p2) fractions prepared from mouse brain. l-glutamate stimulated calcium uptake, but this action was not shared by other amino acids including d-glutamate, l-aspartate, n-methyl-aspartate or kainate. the glutamate-stimulated calcium uptake was, however, blocked by inhibitors of glutamate dehydrogenase, such as d-glutamate, glutarate and triiodothyronine. subcellular fractionation demonstrated that the u ... | 1985 | 2860953 |
| chemotaxis by entamoeba histolytica. | a micropore membrane procedure to assay taxis by entamoeba histolytica is described and the results of studies of responses to a variety of soluble substances, bacteria, an rat colon washings using this procedure are reported. trophozoites migrated in blind well chambers through 8-micron pore size polycarbonate membranes but not nitrocellulose membranes up to 12 micron pore size. amoebae were attracted toward fresh axenic culture medium (tyi-s), an enzymatic hydrolysate of casein (trypticase), a ... | 1985 | 2861283 |
| antibodies to htlv-i p24 in african and portuguese populations. | using a radioimmunoassay to detect htlv-i protein antibodies of molecular weight 24,000, we screened populations from algeria (140 subjects), tunisia (442), mali (69), senegal (415), uganda (135), the central african republic (77), the congo (360), and madagascar (193). only four subjects were positive (1 from senegal, 1 from uganda, 2 from the congo). this is a much lower figure than that found by others in africa by the enzyme-linked immunosorbent assay technique. in addition, 319 portuguese b ... | 1985 | 2861895 |
| transformation of primary cultured and co-cultured adult rat liver cells by 3'-methyl-4-dimethylaminoazobenzene. | under various conditions of culture and carcinogen treatment, the transformation of liver cells by 3'-methyl-4-dimethylaminoazobenzene (3'-me-dab) was studied. primary liver cell (plc) cultures from adult male rats and co-cultures with plcs of arl-d8 cells of a liver epithelial-like clear cell line from adult female rats were treated with 0.24 mm 3'-me-dab for 6 days. four of 8 carcinogen-treated plc cultures contained cells with marker chromosomes, and 3 of the 8 cultures contained gamma-glutam ... | 1985 | 2862759 |
| [relationship between hosts and hemorrhagic fever renal syndrome in chang-an county, shan-xi province]. | 1985 | 2862998 | |
| characterization of enhanced intestinal permeability; electrophysiological study on the effects of diclofenac and ethylenediaminetetraacetic acid. | the mechanism of the enhancing effects of diclofenac sodium and disodium ethylenediaminetetraacetate (edta) on intestinal permeability to sulphanilic acid were investigated in the rat isolated jejunum by an electrophysiological technique. the results suggested that diclofenac sodium increased the mucosal-to-serosal flux rate of sulphanilic acid by enhancing both transcellular and paracellular permeabilities while the effect of edta was located only in the paracellular pathway. | 1985 | 2863363 |
| iga response during accidental infection with shigella flexneri. | 1985 | 2863666 | |
| combination of mefloquine with sulfadoxine-pyrimethamine compared with two sulfadoxine-pyrimethamine combinations in malaria chemoprophylaxis. | 1985 | 2863679 | |
| immunocytochemical studies of somatostatin neurons in brain. | immunohistochemical studies with antisera to somatostatin have, in many instances, led the way to our present understanding of the peptidergic nervous system. somatostatin was among the first of the hypophysiotropic hormones shown to be contained in diverse neuronal circuits outside of the hypothalamus. for example, somatostatin is found within neurons ranging in location from the cerebral cortex to primary sensory neurons to enteric neurons within the gut wall. somatostatin was also the first n ... | 1985 | 2863929 |
| trials with portable screen rooms modified for use as animal-baited net traps for mosquito collection. | trials in larimer county, colorado during july and august 1984, with recreational screen rooms modified as large animal-baited mosquito traps are described. the two units tested are free-standing, portable and require no external support. in all-night trials, 462.5 mosquitoes/trap night were captured with horse bait compared with 367/trap night with cdc light traps. in 2-hour evening comparisons, mosquitoes collected per trapping period totalled 416 for horse-bait traps, 132 for light traps, and ... | 1985 | 2906667 |
| release of atrial natriuretic peptide by atrial distension. | a heterologous radioimmunoassay was used to measure the concentration of immunoreactive atrial natriuretic peptide (ianp) in plasma from the femoral artery of eight chloralose anaesthetized dogs. mitral obstruction which increased left atrial pressure by 11 cmh2o increased plasma ianp from 97 +/- 10.3 (mean +/- se) to 135 +/- 14.3 pg/ml. pulmonary vein distension increased heart rate but did not increase plasma ianp. bilateral cervical vagotomy and administration of atenolol (2 mg/kg) did not pr ... | 1985 | 2931169 |
| thyroxine induced transformation in sarcoplasmic reticulum of rabbit soleus and psoas muscles. | the properties of the sarcoplasmic reticulum membranes isolated from slow-twitch type i soleus and fast-twitch type ii psoas muscles of control and thyroxine treated rabbits were comparatively studied. membrane yield, maximal calcium storing capacity, atp-supported calcium uptake, calcium-dependent atpase activity and calcium-dependent phosphoprotein formation were found to be 3-10 fold higher in psoas than in soleus preparations. membrane yield, calcium-dependent atpase activity, atp-supported ... | 1985 | 2934902 |
| cellular and vessel wall morphology of cerebral cortical arterioles after short-term diabetes in adult rats. | microvascular pathology is one of the major problems associated with prolonged diabetes mellitus. the purpose of the present study was to determine if anatomical cellular pathology of the cerebral cortical microvessels could be detected very early after the onset of a streptozotocin-induced model of diabetes. male rats at age 14-16 weeks were hyperglycemic (greater than 250 mg/dl) for 4 weeks prior to scanning and transmission electron microscopic analysis of the external and internal cell and v ... | 1985 | 2935212 |
| phase shift of daily profiles of n-acetyltransferase in the rat pineal gland. | pineal n-acetyltransferase activity (nat) has a circadian rhythm with peak values in the dark time and low values in the light time. nat time profiles were measured in rats exposed to ld 14:10, to constant dark, and to acute (less than 48 hr) light-dark treatments. in all experiments, imposition of light suppressed nat. the phase of the dark time nat cycle was altered 2 hr or less by the following treatments: 3 hr light in the early subjective night, 3 hr light in the late subjective night, 2 hr ... | 1985 | 3831309 |
| [effect of titanium phosphate, zirconium oxide and a mixture of ammonium phosphotungstenate with zirconium phosphate on the elimination of radioactive cesium from the bodies of rats]. | 1985 | 3835674 | |
| a new partition thin-layer chromatographic method for steroid separations. | tlc plates with a 25 mu thick polyamide stationary phase were modified for the separation of neutral steroids by impregnation with propylene glycol. a mixture of tritiated 5 alpha-androstan-3 alpha,17 beta-diol, testosterone, 17 beta-hydroxy-5 alpha-androstan-3-one and 4-androstene-3,17-dione was applied to the plate and developed in a toluene mobile phase to a height of 13.6 cm. this resulted in complete resolution of the 4 compounds as detected by a gas flow scanner or imaging analyzer. cuttin ... | 1985 | 3835732 |
| isolation and characterization of neuropeptide y from porcine intestine. | the isolation and primary structure of intestinal neuropeptide y (npy) is described. the peptide was purified from porcine intestinal extracts using a chemical assay and radioimmunoassay for npy. the amino acid sequence of this peptide is: tyr-pro-ser-lys-pro-asp-asn-pro-gly-glu-asp-ala-pro-ala-glu-asp-leu-ala- arg-tyr-tyr- ser-ala-leu-arg-his-tyr-ile-asn-leu-ile-thr-arg-gln-arg-tyr-nh2. this the structure of intestinal npy is identical to the npy of brain origin. | 1985 | 3838090 |
| nucleotide sequences of the cdna and an intronless pseudogene for human lactate dehydrogenase-a isozyme. | eight cdna clones for lactate dehydrogenase-a isozyme (ldh-a) were isolated from a human fibroblast cdna library, characterized, and no sequence heterogeneity was found. four cdna clones appear to contain nearly full-length cdna inserts and the complete nucleotide sequence of 1710 base pairs consists of the protein-coding sequence (999 base pairs), the 5' (97 base pairs) and 3' (565 base pairs) untranslated regions and poly(da) tail (49 base pairs). the predicted amino acid sequence of the human ... | 1985 | 3838278 |
| molsidomine on cardiovascular leukotriene d4 actions. | anaesthetized open-chest dogs were used to study the effects of intracoronary leukotriene d4 (ltd4; 0.5 micrograms/kg) on haemodynamics, the electrocardiogram (ecg), coronary blood flow in the left circumflex artery and coronary resistance in the absence or presence of the antianginal drug molsidomine (500 micrograms/kg i.v.). ltd4 increased left ventricular (lv) filling pressure from 6.5 +/- 3.8 to 14.7 +/- 3.2 mm hg (p less than 0.01), decreased lv dp/dtmax from 2,500 +/- 200 to 1,240 +/- 205 ... | 1985 | 3839292 |
| myosin light chain phosphorylation and isometric twitch potentiation in intact human muscle. | the effects of a single voluntary contraction of the quadriceps muscle group on phosphate incorporation into the phosphorylatable light chains (p-light chains) of fast and slow myosin isolated from the vastus lateralis muscle and potentiation of the electrically stimulated twitch tension was studied in intact human muscle. twitch potentiation was maximal 20 s after the voluntary contraction. thereafter, twitch potentiation declined, but was still significantly higher than pre-contraction values ... | 1985 | 3839303 |
| heterogeneity of membrane phospholipid mobility in endothelial cells depends on cell substrate. | cellular growth control and differentiation have been shown to be dependent on both cell-cell and cell-substrate contacts. interactions of cells with extracellular material are critical events during embryonic development and maintenance of tissue function. plasma membrane receptors have been described for components of the extracellular matrix such as fibronectin, laminin and various collagen types. transmembrane signalling has been shown to be influenced by the lateral mobilities of the plasma ... | 1985 | 3839906 |
| 7-hydroxyguanine, a novel antimetabolite from a strain of streptomyces purpurascens. i. taxonomy of producing organism, fermentation, isolation and biological activity. | strain a-347, an actinomycete isolated from a soil sample, was found to produce a new antimetabolite, 7-hydroxyguanine. the aerial mycelium formed spiral spore chains with spiny spore surface. the chemical composition of strain a-347 indicated that it was an actinomycete of cell wall type i. from its morphological, cultural, physiological characteristics and direct comparison with the type culture, this strain was classified as streptomyces purpurascens. 7-hydroxyguanine was purified from the br ... | 1985 | 3840157 |
| isolation and hypoglycemic activity of ganoderans a and b, glycans of ganoderma lucidum fruit bodies. | 1985 | 3840903 | |
| tactile cutaneous representation in cerebellar granule cell layer of the opossum, didelphis virginiana. | recent studies of the albino rat revealed extensive cutaneous somatosensory projections to the granule cell (gc) layer of the cerebellar hemispheres and the caudal vermis. these projections are organized asomatotopically in patchy mosaics. to determine whether similar projections exist in a marsupial, we explored the gc layer of the cerebellar cortex of anesthetized virginia opossums using in-depth microelectrode micromapping and juxtathreshold cutaneous natural stimulation techniques. we found: ... | 1985 | 3843070 |
| powerline frequency electric and magnetic fields: a pilot study of risk perception. | the perception of the potential risk arising from human exposure to 50/60 hz electric and magnetic fields was studied with a quasi-random sample of 116 well-educated, opinion leaders using the risk perception framework previously developed by slovic, fischhoff, and lichtenstein. these individuals rated exposure to fields from transmission lines and electric blankets on a variety of scales that have been found useful in characterizing people's risk attitudes and perceptions. these judgments allow ... | 1985 | 3843677 |
| single-step purification of "kallikrein-resistant" kininogen from rat plasma using monoclonal-antibody immunoaffinity chromatography. | monoclonal antibody to rat plasma kininogen, obtained after immunization of mice with the kininogen prepared by conventional methods, was purified from ascites fluid and coupled to cnbr-activated sepharose-4b. monoclonal-antibody affinity adsorbant thus prepared provided a rapid single-step method of purifying to homogeneity plasma kininogen. purified rat plasma kininogen showed identical molecular weight and immunological cross-reactivity to rat plasma low molecular weight (lmw) kininogen purif ... | 1985 | 3851460 |
| recognition of a leukemia-related antigen by an antiidiotypic antiserum to an anti-gp70 monoclonal antibody. | the possibility that receptors for retroviral gp70 share structural elements with the antigen-binding sites of anti-retroviral gp70 antibodies was investigated. a monoclonal antibody (1416) was produced that reacted with the gp70 of a cloned recombinant leukemogenic retrovirus, termed p1. an antiidiotypic antiserum raised to 1416 was tested for its ability to bind to the thymic leukemia induced by p1 (p1 thy). a membrane structure was identified on the surface of p1 thy that reacted with the ant ... | 1985 | 3856626 |
| immigrants and a public oral health care service for children in denmark. | the present study was conducted to compare level of dental caries, treatment pattern and contact patterns with the child oral health care system of children of immigrants. the study comprised 239 turkish, 117 pakistani and 91 other immigrants and a control group of 359 danish children. data were extracted from the dental records in the clinic where they were treated. all groups of immigrants showed more caries in the primary dentition and less caries in the permanent dentition than danish childr ... | 1985 | 3860330 |
| cholecystokinin potentiation of dopamine-mediated behaviors in the nucleus accumbens. | 1985 | 3861122 | |
| [morphological changes in young rat mandible after release of muscle function during growth]. | 1985 | 3864900 | |
| protein loss of bovine dental enamel during in-vitro subsurface demineralization. | a chemical system based on the dialysis principle was used to study protein loss of dental enamel during demineralization with an acetic-acid buffer solution containing calcium and phosphate, in which the fluoride-ion activity was kept constant. this resulted in a subsurface lesion, with a depth of about 130 microns. after demineralization, protein material was isolated from the demineralization solution. u.v. spectra of the protein showed strong absorbance between 240 and 300 nm. amino-acid com ... | 1985 | 3865639 |
| [adaptation of rhodopseudomonas palustris to changes in atmospheric conditions]. | the high level of delta-aminolaevulinate synthetase (ala-s) in rhodopseudomonas palustris cells grown under photosynthetic conditions decreased when they were vigorously aerated; moreover, the bacteriochlorophyll synthesis suddenly stopped. when aeration was interrupted, enzymatic level increased notably, but bacteriochlorophyll level did not. chloramphenicol added to cultures when aeration was interrupted did not affect the increase of enzymic level. when it was added as the aeration started, t ... | 1985 | 3870744 |
| reactivities of neoplastic t cells to human autoantibody and monoclonal antibodies. | 1985 | 3873778 | |
| antifibrillatory actions of bepridil and butyl-mdi, two intracellular calcium antagonists. | the antifibrillatory and electrophysiologic actions of bepridil and butyl-methylenedioxyindene (bu-mdi), two intracellular calcium antagonists, were examined in anesthetized dogs. the administration of bepridil (1.0-10.0 mg/kg i.v.) significantly increased the electrical threshold for ventricular fibrillation determined during unobstructed coronary flow, and was associated with a significant decrease in ventricular excitability and a progressive depression in ventricular myocardial conduction. b ... | 1985 | 3874780 |
| isolation and characterization of epidermal growth factor from human milk. | epidermal growth factor (egf) has been purified from human milk. the purification was monitored with a human placental membrane radioreceptor assay using murine salivary epidermal growth factor i (megf i) as a competitive ligand and was achieved exclusively by the use of reverse-phase liquid chromatography (rplc). the sequential use of preparative, semipreparative and analytical rplc on an octylsilica support with solvent systems of different solute selectivity such as pyridine formate, triethyl ... | 1985 | 3874788 |
| role of the posterior capsule in the prevention of postoperative bacterial endophthalmitis: experimental primate studies and clinical implications. | the posterior capsule has an important effect on the risk of postoperative bacterial endophthalmitis. in order to investigate whether the posterior capsule inhibited the spread of infection into the vitreous we performed extracapsular cataract extraction in both eyes of 10 primates. in one eye of each primate the posterior capsule was left intact and in the other eye a large posterior capsulectomy was performed. when the anterior chambers were challenged with equivalent inocula of staphylococcus ... | 1985 | 3877524 |
| pathophysiology of the kidney in rats with heymann nephritis. | alterations in kidney function were assessed early in the course of heymann nephritis that was induced in rats by immunization with fx1a, an extract prepared from rat kidney cortex. whole kidney and single nephron function were evaluated by clearance and micropuncture techniques. kidney function was studied in stage 1 of heymann nephritis, before the onset of proteinuria, and in stage 2, when antibodies are deposited along the brush border of proximal tubules. although overall kidney function wa ... | 1985 | 3877841 |
| clinical studies with corticotropin releasing factor: implications for the diagnosis and pathophysiology of depression, cushing's disease, and adrenal insufficiency. | corticotropin releasing factor (crf) is a 41 amino acid peptide first isolated from sheep hypothalami and thought to be a principal modulator of the hypothalamic-pituitary-adrenal cortical (hpa) axis. we report herein a series of clinical studies with crf in healthy volunteers and in patients with abnormalities in hpa function, including depression, cushing's disease, cushing's syndrome, and addison's disease. our data indicate that crf can be a diagnostic aid in distinguishing various disorders ... | 1985 | 3878972 |
| aspartate: 2-oxoglutarate aminotransferase from trichomonas vaginalis. identity of aspartate aminotransferase and aromatic amino acid aminotransferase. | aspartate: 2-oxoglutarate aminotransferase from the anaerobic protozoon trichomonas vaginalis was purified to homogeneity and characterized. it is a dimeric protein of overall mr approx. 100000. only a single isoenzyme was found in t. vaginalis. the overall molecular and catalytic properties have features in common with both the vertebrate cytoplasmic and mitochondrial isoenzymes. the purified aspartate aminotransferase from t. vaginalis showed very high rates of activity with aromatic amino aci ... | 1985 | 3879173 |
| effect of octanoate on blood-brain barrier permeability using l-dopa as a marker. | short-chain fatty acid injection into animals produces coma, seizures, and hyperventilation. one mechanism of coma production may be through alterations in membrane permeability characteristics. the spg histofluorescence technique was used to evaluate changes in blood-brain barrier permeability to injected l-dopa in rats after intraperitoneal injections of the short-chain fatty acid, sodium octanoate. diffusion of intravascular fluorescence was observed around brain capillaries in the octanoate- ... | 1985 | 3880408 |
| effect of octanoate on blood-brain barrier permeability using l-dopa as a marker. | short-chain fatty acid injection into animals produces coma, seizures, and hyperventilation. one mechanism of coma production may be through alterations in membrane permeability characteristics. the spg histofluorescence technique was used to evaluate changes in blood-brain barrier permeability to injected l-dopa in rats after intraperitoneal injections of the short-chain fatty acid, sodium octanoate. diffusion of intravascular fluorescence was observed around brain capillaries in the octanoate- ... | 1985 | 3880408 |
| effect of antigen form on local immunoglobulin a memory response of intestinal secretions to shigella flexneri. | an enhanced memory response, as shown by increased titers of specific immunoglobulin a (iga), was seen in intestinal secretions from isolated thiry-vella loops in rabbits primed orally with live, locally invasive shigella sp. x16 and challenged 60 days later with a single oral dose of the same antigen. heat-killed shigella preparations, when used as either the priming or challenge antigen, did not elicit such a memory response in this system. in the present study, the role of antigen form and do ... | 1985 | 3880720 |
| an animal model of haemolytic--uraemic syndrome in shigellosis: lipopolysaccharides of shigella dysenteriae i and s. flexneri produce leucocyte-mediated renal cortical necrosis in rabbits. | to develop an animal model of the haemolytic-uraemic syndrome during shigellosis, rabbits were injected with lipopolysaccharides (lps) extracted by the hot phenol-water method from shigella dysenteriae i and from s. flexneri. two intravenous injections of lps spaced by 24 h elicited renal cortical necrosis in a generalized shwartzman reaction characterized by fibrin deposition in glomerular capillaries and by elevated plasma creatinine concentration. rabbits rendered leucopenic by busulphan trea ... | 1985 | 3882120 |
| vesicles and cisternae in the trans golgi apparatus of human fibroblasts are acidic compartments. | recently we demonstrated that low-ph compartments can be visualized with the electron microscope using a basic congener of dinitrophenol, 3-(2,4-dinitroanilino)-3'-amino-n-methyldipropylamine (damp), which concentrates in acidic compartments and can be detected by immunocytochemistry with a monoclonal anti-dinitrophenol antibody. we now report that damp also accumulates in cisternae and vesicles associated with the trans face of the golgi apparatus. damp rapidly leaves this compartment when cell ... | 1985 | 3882239 |
| evidence for proctolin-like substances in the central nervous system of the leech hirudo medicinalis. | the distribution of proctolin-like immunoreactive (pli) cells was mapped in the central nervous system of the leech hirudo medicinalis. in segmental ganglia, pli cells can be divided into two groups: cells that stain repeatedly in every successive ganglion, and cells that stain only in specific segmental ganglia. the number of pli cells, therefore, ranged from eight to 20 cells per ganglion. one bilateral pair of pli cells (cells pli-1) was further characterized morphologically by lucifer yellow ... | 1985 | 3882776 |
| fatal fungemia with arthritic involvement caused by trichosporon beigelii in a bone marrow transplant recipient. | 1985 | 3882853 | |
| rabbit antisera against three different bacteria which can induce reactive arthritis: analysis by elisa, immunoprecipitation and western blot. | three strains of bacteria which induce reactive arthritis were collected: a shigella flexneri, designated 7060; another sh. flexneri, designated 316; and a yersinia enterocolitica of serotype 03. rabbit antisera were generated against each of them to test for the extent and nature of cross-reactivity among these strains. when analysed by the elisa technique, antisera against 7060 and 316 showed strong cross-reactivity with y. enterocolitica. in contrast, the reaction of antisera prepared against ... | 1985 | 3884493 |
| plaque formation by virulent shigella flexneri. | an in vitro tissue culture plaque assay was developed to investigate the intracellular replication and intercellular spread of virulent shigellae. shigella plaques were formed in hela cell monolayers in the presence of an agarose overlay containing tissue culture medium and gentamicin, which eliminated extracellular bacterial growth. microscopically, the plaques were characterized by a central area of dead host cells surrounded by cells infected with shigellae. cells further away from the plaque ... | 1985 | 3884506 |
| cloning the gene for congo red binding in shigella flexneri. | the ability to bind the dye congo red from agar medium is associated with virulence of shigella species. dna sequences conferring this property have been cloned from a large, 140-kilobase plasmid of shigella flexneri into a plasmid vector. this recombinant plasmid does not fully restore virulence to s. flexneri isolates which have lost the large plasmid. this indicates that other genes present on the 140-kilobase plasmid must also be required for virulence of s. flexneri. the cloned fragment con ... | 1985 | 3884508 |
| shigella flexneri bacteraemia. | shigellosis is usually a non-invasive enteric disease, rarely accompanied by extra-intestinal manifestations. shigella septicaemia is therefore reported in a child aged 10 months. in the laboratory the organism was resistant to ampicillin and only moderately susceptible to chloramphenicol. the patient responded well to cotrimoxazole to which the organism isolated was susceptible in vitro. we recommend that blood as well as faeces should be cultured in exceptionally ill patients with suspected or ... | 1985 | 3884717 |
| effect of temperature on the circular dichroism spectra of beta 2-microglobulins. | when the temperature was lowered from 25 to 5 degrees c dramatic changes were observed in the near-ultraviolet circular dichroism spectra of bovine and caprine but not human beta 2-microglobulin. comparison of the protein sequences suggests that the conformational change occurs in the amino-terminal 24 residues and that a tyrosine residue located on a potential beta-turn acts as a reporter group. because delta h degrees is small (-22 kcal x mol-1), such conformational changes, possibly not readi ... | 1985 | 3886425 |
| studies of metabolites in diarrheal stool specimens containing shigella species by frequency-pulsed electron capture gas-liquid chromatography. | eleven diarrheal stool specimens and 10 control stool specimens from cairo, egypt, were studied by frequency-pulsed electron capture gas-liquid chromatography (fpec-glc). four cases involving shigella sonnei, three cases involving shigella boydii, and four cases involving shigella flexneri were studied. the aqueous stools were centrifuged, extracted with organic solvents, and derivatized to form specific electron-capturing derivatives of carboxylic acids, alcohols, hydroxy acids, and amines. ana ... | 1985 | 3886695 |
| perturbation of insulin oscillations by nerve blockade in the in vitro canine pancreas. | the in vitro canine pancreas produces an oscillatory pattern of insulin secretion during a constant glucose concentration despite the lack of external nervous modulation or recirculating hormone feedback. the normal period of insulin fluctuations (7.4 +/- 0.34 min) is unaffected by combined adrenergic and cholinergic blockade by 5 microm atropine, 4 microm propranolol, and 4 microm phentolamine (8.0 +/- 0.31 min, p less than 0.20). to test the theory that the coordination of islet secretion may ... | 1985 | 3887939 |
| erythema nodosum associated with shigella colitis. | 1985 | 3888122 | |
| survival of bacterial enteropathogens in the ice of popular drinks. | we examined the survival of four bacterial enteropathogens frozen in ice and subsequently allowed to melt in various popular drinks. the counts of all the organisms were markedly lowered by freezing alone, and the numbers were further decreased by exposure to some of the drinks. nevertheless, none of the organisms were completely eliminated as a result of freezing for 24 hours followed by melting in any of the test drinks, even when the drink was 86-proof tequila. | 1985 | 3889393 |
| [shigella serotypes isolated july-october 1983 and their antibiotic sensitivity]. | twenty four strains of shigella were isolated from the stool of patients who had been admitted to the hospital with the complaint of diarrhea. of these, 17 were found to be sh. flexneri, and 7 sh. boydii. antibiotic susceptibility tests indicated that all of the 24 isolates probably had multiple resistance factors. twenty three of 24 strains were found to be resistant to ampicillin. this may be important from a therapeutic point of view. | 1985 | 3892249 |
| [the shigella flexneri epidemic of 1983/84: the lesson to be learned from this]. | 1985 | 3892308 | |
| [antibiotic resistance in the isolates of shigella flexneri type 2 associated with the "shrimp epidemic"]. | 1985 | 3892309 | |
| [5 cases of reactive arthritis following a shigella flexneri epidemic in the netherlands]. | 1985 | 3892310 | |
| [bacillary dysentery in utrecht: 2 epidemics]. | 1985 | 3892311 | |
| [clinical observations during an epidemic caused by shigella flexneri]. | 1985 | 3892312 | |
| [detection of shigella antigens in the feces of patients with acute intestinal diseases using a coagglutination reaction]. | a total of 113 patients with acute intestinal diseases have been examined with the use of the coagglutination test. 84.95% of the patients showed the presence of different shigella antigens. in patients with bacteriologically confirmed dysentery the corresponding shigella antigens were detected in 96.97% of cases in s. sonnei dysentery, in 90% of cases in s. flexneri dysentery, in 75% of cases in s. newcastle dysentery and in 100% of bases in s. boydii dysentery. in 81.6% of patients with acute ... | 1985 | 3892986 |
| [possible production of 2 cytotoxins by a strain of escherichia coli]. | 1985 | 3893462 | |
| humoral response of the mouse to treponema pallidum. | to investigate the development of the humoral immune response of mice to infection with treponema pallidum, balb/c and c57bl mice were injected intradermally with 10 x 10(6) virulent organisms. serum samples were taken from the mice at weekly intervals after infection and used in the electrophoretic transblotting technique to detect t pallidum and t phagedenis biotype reiter antigens. the serum samples taken from infected mice at 21, 42, 84, and 126 days after infection recognised two to 15 t pa ... | 1985 | 3894212 |
| current diagnosis of infective endocarditis. | an analysis was made of 91 cases of infective endocarditis (ie) with regard to causative organisms and their sensitivities to various antibiotics, the clinical features of the disease, the laboratory test results and other items were important in establishing a diagnosis of ie. the number of cases of ie has shown a tendency to increase in recent years, particularly in the number of elderly patients, and the ratio of total cases consisting of prosthetic valve endocarditis (pve) has shown a sharp ... | 1985 | 3894710 |