Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| changes in translatable mrna populations induced in rice seedlings by exposure to freeze-thaw stress. | in order to examine whether changes in gene expression are induced by injury in seedlings of rice (oryza sativa) after a lethal freeze-thaw stress. mrnas were extracted and their in vitro translation products were compared with those of the control plants by polyacrylamide gel electrophoresis. an mrna encoding an entirely new protein with m(r) of ca. 37 kda was detected as early as 30 min of thawing at 28 degrees c. two-dimensional gel electrophoresis revealed the induction of mrnas for at least ... | 1993 | 8485458 |
| use of bar as a selectable marker gene and for the production of herbicide-resistant rice plants from protoplasts. | we have used the bar gene in combination with the herbicide basta to select transformed rice (oryza sativa l. cv. radon) protoplasts for the production of herbicide-resistant rice plants. protoplasts, obtained from regenerable suspension cultures established from immature embryo callus, were transformed using peg-mediated dna uptake. transformed calli could be selected 2-4 weeks after placing the protoplast-derived calli on medium containing the selective agent, phosphinothricin (ppt), the activ ... | 1993 | 8467080 |
| nucleotide sequence of a rice (oryza sativa) prolamin storage protein gene, rp6. | 1993 | 8310050 | |
| cdna cloning and sequence analysis of a pathogen-induced thaumatin-like protein from rice (oryza sativa). | 1993 | 8310049 | |
| phylogenetic distribution and genetic mapping of a (ggc)n microsatellite from rice (oryza sativa l.). | dna microsatellites are ubiquitously present in eukaryotic genomes and represent a vast source of highly informative markers. we describe in this article a (ggc)n microsatellite which is widely distributed in eukaryotic genomes. using polymerase chain reaction (pcr) techniques and dna sequencing, we demonstrated for the first time in plant species that a (ggc)n microsatellite locus is moderately polymorphic. six alleles are present at this locus in rice and length polymorphisms are caused by var ... | 1993 | 8448360 |
| a transcription map of the chloroplast genome from rice (oryza sativa). | the complete nucleotide sequence (134,525 bp) of the chloroplast genome from rice (oryza sativa l.) contains four rrna genes, 30 trna genes, and over 100 genes that encode proteins. however, expression of only a few of these genes, namely psba, rbcl, and atpb/e, has been detected. we constructed the complete transcription map of rice chloroplast dna by northern hybridization of total rna from rice seedlings, using subclones from a clone bank of rice chloroplast dna as probes. approximately 90% o ... | 1993 | 8381719 |
| cytosolic triosephosphate isomerase is a single gene in rice. | a cdna clone encoding rice (oryza sativa l.) cytosolic triosephosphate isomerase (tpi), an important glycolytic enzyme, was isolated and characterized. the clone (prtpi-6) contains an open reading frame of 759 base pairs, encoding a polypeptide chain of 253 amino acid residues (m(r) 27,060). the identity of this clone was defined by its high homology (85% nucleotide sequence and 89% amino acid sequence identical match) with a maize mrna sequence encoding the cytosolic tpi and with tpis from othe ... | 1993 | 8278510 |
| a major photoperiod-sensitivity gene tagged with rflp and isozyme markers in rice. | photoperiod-sensitive rice (oryza sativa l.) cultivars are widely grown in rainfed lowland areas with unfavorable water regimes. a molecular marker for the trait would be useful in genetic and physiological studies and in developing improved photoperiod-sensitive cultivars. previous genetic studies identified a major gene for photoperiod sensitivity on chromosome 6. we have tested an isozyme marker and several rflp probes mapping to chromosome 6 in an attempt to identify marker(s) tightly linked ... | 1993 | 24195926 |
| state of the foreign gene and of the genome in transgenic rice (oryza sativa l.). | pcr with random primers (rapd analysis) performed on the dna of embryogenic and non-embryogenic suspension cultured rice and of transformed rice plants allows the evaluation of the extent of dna changes in the different biological materials. this is thus suggested as a convenient approach, in combination with restriction analysis and southerns blotting, to evaluate the integrity of the foreign gene, the stability of the insertion site and the stability of the whole genome. | 1993 | 22358675 |
| identification of the entire set of transferred chloroplast dna sequences in the mitochondrial genome of rice. | the entire set of transferred chloroplast dna sequences in the mitochondrial genome of rice (oryza sativa cv. nipponbare) was identified using clone banks that cover the chloroplast and mitochondrial genomes. the mitochondrial fragments that were homologous to chloroplast dna were mapped and sequenced. the nucleotide sequences around the termini of integrated chloroplast sequences in the rice mtdna revealed no common sequences or structures that might enhance the transfer of dna. sixteen chlorop ... | 1993 | 8437578 |
| responses to continuous and discontinuous nacl stress of long-term cultured rice (oryza sativa l.) cells. | rice (oryza sativa l.) cells, grown under a continuous stress of 1.5% nacl, produced a homogeneous mass of dry, compact, nodular callus with high regeneration potential, typical of embryogenic cultures. when transferred and subcultured in conditions without salt, the pre-adapted cells underwent several changes, leading to the formation of heterogeneous populations that comprised different cell types. this newly formed callus, in the 6-month period without salt stress, resembled cell populations ... | 1993 | 8184630 |
| state of the foreign gene and of the genome in transgenic rice (oryza sativa l.). | pcr with random primers (rapd analysis) performed on the dna of embryogenic and non-embryogenic suspension cultured rice and of transformed rice plants allows the evaluation of the extent of dna changes in the different biological materials. this is thus suggested as a convenient approach, in combination with restriction analysis and southerns blotting, to evaluate the integrity of the foreign gene, the stability of the insertion site and the stability of the whole genome. | 1993 | 7763739 |
| fertile indica and japonica rice plants regenerated from protoplasts isolated from embryogenic haploid suspension cultures. | a system to regenerate fertile rice (oryza sativa l.) plants (both indica and japonica varieties) from protoplasts isolated from anther-derived embryogenic haploid suspension cultures has been established. green plants were regenerated from protoplast-derived cell clusters five months after suspension culture initiation. protoplast yields and subsequent growth of the protoplast-derived microcalli were enhanced by transferring suspension cells into aa medium (muller et al. 1978) three to four day ... | 1992 | 24201730 |
| distinctive responses of ribulose-1,5-bisphosphate carboxylase and carbonic anhydrase in wheat leaves to nitrogen nutrition and their possible relationships to co(2)-transfer resistance. | the amounts of ribulose-1,5-bisphosphate carboxylase/oxygenase (rubisco), total chlorophyll (chl), and total leaf nitrogen were measured in fully expanded, young leaves of wheat (triticum aestivum l.), rice (oryza sativa l.), spinach (spinacia oleracea l.), bean (phaseolus vulgaris l.), and pea (pisum sativum l.). in addition, the activities of whole-chain electron transport and carbonic anhydrase were measured. all plants were grown hydroponically at different nitrogen concentrations. although ... | 1992 | 16653191 |
| changes in cytokinin activities and mass spectrometric analysis of cytokinins in root exudates of rice plant (oryza sativa l.) : comparison between cultivars nipponbare and akenohoshi. | changes in exudation rate and cytokinin activities in the exudates were measured in two varieties of rice (oryza sativa l.), cv nipponbare (a japanese normal cultivar) and cv akenohoshi (a high-yielding cultivar). the exudation rates of akenohoshi, the leaves of which remained green for a longer time, were higher than those of nipponbare after the booting stage. cytokinin activities in the exudates of akenohoshi were higher than those of nipponbare during the ripening period. cytokinins in the e ... | 1992 | 16653189 |
| isolation of genes abundantly expressed in rice anthers at the microspore stage. | a cdna library of rice (oryza sativa l.) has been constructed from anthers at an early stage of pollen development. by differential screening of the library, we have isolated cdnas of two genes, designated as osc4 and osc6, that are abundantly expressed in anthers containing tetrads and uninucleate microspores, but are not expressed in leaves or roots. expression of osc4 is absent in mature anthers, while osc6 is present although the expression decays during pollen maturation. a comparison of th ... | 1992 | 1463854 |
| plant regeneration from haploid cell suspension-derived protoplasts of mediterranean rice (oryza sativa l. cv. miara). | more than 750 plants were regenerated from protoplasts isolated from microspore callus-derived cell suspensions of the mediterranean japonica rice miara, using a nurse-feeder technique and n6-based culture medium. the mean plating efficiency and the mean regeneration ability of the protocalluses were 0.5% and 49% respectively. flow cytometric evaluation of the dna contents of 7 month old-cell and protoplast suspensions showed that they were still haploid. contrastingly, the dna contents of leaf ... | 1992 | 24213364 |
| effect of anoxia on starch breakdown in rice and wheat seeds. | the capabilities of rice (oryza sativa l.) and wheat (triticum aestivum l.) seeds (caryopses) to degrade starchy reserves present in the endosperm tissue were compared under anaerobic conditions. the results showed that rice, a species highly tolerant to anoxia, can readily break down starch under anaerobiosis concomitant with germination, while wheat does not germinate and fails to degrade starch present in the endosperm. this clearly distinct behavior is likely the consequence of the successfu ... | 1992 | 24178396 |
| tissue distribution of glutamate synthase and glutamine synthetase in rice leaves : occurrence of nadh-dependent glutamate synthase protein and activity in the unexpanded, nongreen leaf blades. | to further explore the function of nadh-dependent glutamate synthase (gogat), the tissue distribution of nadh-gogat protein and activity was investigated in rice (oryza sativa l.) leaves. the distributions of ferredoxin (fd)-dependent gogat, plastidic glutamine synthetase, and cytosolic glutamine synthetase proteins were also determined in the same tissues. high levels of nadh-gogat protein (33.1 mug protein/g fresh weight) and activity were detected in the 10th leaf blade before emergence. the ... | 1992 | 16653141 |
| seed dormancy in red rice : viii. embryo acidification during dormancy-breaking and subsequent germination. | exposure of dehulled, dormant red rice (oryza sativa) seeds to dormancy-breaking treatments (10 mm sodium nitrite, 20 mm propionic acid, 30 mm methyl propionate, 40 mm propionaldehyde, or 70 mmn-propanol) induced tissue ph acidification during chemical contact at least 12 h before visible germination. during chemical contact, the onset of embryo acidification occurred before or coincident with the chemical contact interval necessary for subsequent germination. upon seed transfer to h(2)o followi ... | 1992 | 16653105 |
| herbicide-resistant indica rice plants from irri breeding line ir72 after peg-mediated transformation of protoplasts. | the commercially important indica rice cultivar oryza sativa cv. ir72 has been transformed using direct gene transfer to protoplasts. peg-mediated transformation was done with two plasmid constructs containing either a camv 35s promoter/hph chimaeric gene conferring resistance to hygromycin (hg) or a camv 35s promoter/bar chimaeric gene conferring resistance to a commercial herbicide (basta) containing phosphinothricin (ppt). we have obtained so far 92 hgr and 170 pptr ir72 plants from protoplas ... | 1992 | 1333295 |
| simple dehydration treatment promotes plantlet regeneration of rice (oryza sativa l.) callus. | to increase plantlet regeneration frequency, rice callus was dehydrated in a petri dish with a single layer of filter paper prior to transfer to the regeneration medium. with a 24 h dehydration treatment, the regeneration frequency was increased to 47 %, while the regeneration frequency of the untreated control was less than 5 %. this relatively simple method provides an alternative method for improving the regeneration frequency of rice callus. | 1992 | 24213284 |
| gibberellin-induced growth and regulation of the cell division cycle in deepwater rice. | excised stem sections of deepwater rice (oryza sativa l., cv. pin gaew 56) containing the highest internode were used to study induction of rapid internodal elongation by gibberellin. it has been shown previously that this growth response is based on an increased cell production rate in the intercalary meristem and on increased cell elongation. our investigations were aimed at establishing the temporal sequence of these ga-regulated processes. cell sizes were determined by scanning electron micr ... | 1992 | 24178326 |
| variation in free-radical damage in rice cell suspensions with different embryogenic potentials. | levels of free-radical-mediated lipid peroxidation were monitored in cell-suspension cultures of oryza sativa l. possessing different embryogenic potentials. oxidative stress was evaluated using assays which sequentially assessed the stages of lipid peroxidation (diene conjugation, peroxidation, and the formation of secondary lipid-peroxidation products). lipid peroxidation was significantly higher in a cell line which had lost embryogenic ability compared with lines which still retained this ca ... | 1992 | 24178318 |
| nucleotide sequence of a cdna encoding a beta subunit of the mitochondrial atpase from rice (oryza sativa). | 1992 | 1387558 | |
| chromosome location of oryza sativa recombination linkage groups. | in situ hybridization, a powerful tool for the molecular cytogeneticist, can be used to physically map repetitive, low-copy, and unique dna sequences in plant chromosomes. with the availability of a recombination map in oryza sativa l. and an improved in situ hybridization technique, this study was designed to establish the relationship between the genetic and physical distances of the rice restriction fragment length polymorphism map. analysis indicated that considerable variation can exist bet ... | 1992 | 1356266 |
| identification of specific proteins from seed embryos by two-dimensional gel electrophoresis for the discrimination between indica and japonica rice. | proteins extracted from seed embryos of 29 different cultivated rice (oryza sativa l.) and one wild rice (o. rufipogon griff.) were compared by two-dimensional gel electrophoresis analysis. among more than 300 protein spots on the gel we found some interesting variations in ten spots which were individually designated as proteins a-j. protein e was observed in all indica cultivars but was not found in those of the subspecies japonica. in contrast, protein f was only detected in japonica cultivar ... | 1992 | 24201498 |
| segregation distortion via male gametes in hybrids between indica and japonica or wide-compatibility varieties of rice (oryza sativa l). | one or two marker genes on each of chromosomes 3, 4, 6, 7, 8, 11 and 12 of the 12 rice chromosomes were tested for segregation distortion in indica-japonica hybrids. marker genes on chromosomes 3, 7, 8, 11 and 12 showed clear segregation distortion. this distortion was not related to the proportion of normal pollen. the germinability of the pollen was less than 10% in the hybrids, although 45-55% of the pollen grains appeared to be morphologically normal. the frequent occurrence of segregation d ... | 1992 | 24201479 |
| ethylene-induced polyamine accumulation in rice (oryza sativa l.) coleoptiles. | effects of ethylene on free polyamine biosynthesis in rice (oryza sativa l. cv taichung native 1) coleoptiles were investigated in sealed and aerobic conditions. in sealed conditions, putrescine increased significantly and coincided with ethylene accumulation. application of ethylene in sealed containers promoted putrescine accumulation over that in sealed controls. this ethylene-enhanced putrescine accumulation was inhibited by the ethylene action inhibitor 2,5-norbornadiene at 4000 mul/l. in a ... | 1992 | 16652953 |
| the amino acid sequence of a 20 kda bifunctional subtilisin/alpha-amylase inhibitor from bran [correction of brain] of rice (oryza sativa l.) seeds. | a 20 kda bifunctional inhibitor of the microbial proteinase, subtilisin, and the alpha-amylase from the larvae of the red flour beetle (tribolium castaneum) was purified from bran of rice seeds by saline extraction, precipitation with ammonium sulphate, ion-exchange chromatography on deae-cellulose and toyopearl cm-650, and preparative hplc on vydac c18. the complete primary structure was determined by automatic degradation of the intact, reduced and s-alkylated protein, and by manual dabitc/pit ... | 1992 | 1511747 |
| rflp analysis of rice (oryza sativa l.) introgression lines. | fifty-two introgression lines (bc2f8) from crosses between two oryza sativa parents and five accessions of o. officinalis were analyzed for the introgression of o. officinalis chromosome segments. dna from the parents and introgression lines was analyzed with 177 rflp markers located at approximately 10-cm intervals over the rice chromosomes. most probe/enzyme combinations detected rflps between the parents. of the 174 informative markers, 28 identified putative o. officinalis introgressed chrom ... | 1992 | 24201348 |
| two oryza sativa genomic dna clones encoding 16.9-kilodalton heat-shock proteins. | 1992 | 16669102 | |
| methyl jasmonate, calcium, and leaf senescence in rice. | the possible involvement of calcium in the regulation of methyl jasmonate-promoted senescence of detached rice (oryza sativa) leaves was investigated. calcium effectively reduced methyl jasmonate-promoted senescence of detached rice leaves. the effect of methyl jasmonate on the senescence was also significantly reduced by calcium ionophore a23187. methyl jasmonate-promoted senescence of detached rice leaves may be mediated through blocking the entrance of calcium ions into the cytosol. | 1992 | 16669094 |
| vascular bundle-specific localization of cytosolic glutamine synthetase in rice leaves. | tissue localizations of cytosolic glutamine synthetase (gs1; ec 6.3.1.2), chloroplastic gs (gs2), and ferredoxin-dependent glutamate synthase (fd-gogat; ec 1.4.7.1) in rice (oryza sativa l.) leaf blades were investigated using a tissue-print immunoblot method with specific antibodies. the cross-sections of mature and senescent leaf blades from middle and basal regions were used for tissue printing. the anti-gs1 antibody, raised against a synthetic 17-residue peptide corresponding to the deduced ... | 1992 | 16669062 |
| characterization of cis-acting elements regulating transcription from the promoter of a constitutively active rice actin gene. | the promoter of the constitutively expressed rice (oryza sativa) actin 1 gene (act1) is highly active in transformed rice plants (w. zhang, d. mcelroy, and r. wu, plant cell 3:1150-1160, 1991). a region 834 bp upstream of the act1 transcription initiation site contains all the regulatory elements necessary for maximal gene expression in transformed rice protoplasts (d. mcelroy, w. zhang, j. cao, and r. wu, plant cell 2:163-171, 1990). we have constructed a series of act1 promoter deletions fused ... | 1992 | 1630454 |
| molecular cloning and characterization of gravity specific cdna in rice (oryza sativa l.) suspension callus. | rice (oryza sativa l. var. nipponbare) suspension callus was exposed to gravity stress at 450,000 g for 2 hours, after which poly(a)+rna was isolated and a cdna library was constructed. three different gravity specific cdnas, namely, gsc 128, gsc 233 and gsc 381 of 0.67, 0.60 and 0.68 kilobase pairs and transcripts of 1.9, 1.6 and 2.0 kb, respectively, were isolated by differential screening and northern hybridization. the maximum level of transcript was achieved after 4 hours of exposure to gra ... | 1992 | 1363521 |
| molecular cloning and characterization of genes related to chilling tolerance in rice. | polyadenylated rna was isolated and a cdna library constructed from seedlings of a chilling-tolerant rice cultivar (oryza sativa l. subsp. japonica cv nipponbare). four clones were isolated by differential screening. northern blot hybridization using rnas from chilling-tolerant (nipponbare) and -sensitive (ir36) cultivars revealed higher steady-state levels of transcripts for the four genes in nipponbare than in ir36 maintained at the same low temperatures. the accumulation of transcripts homolo ... | 1992 | 16668981 |
| efficient fertile plant regeneration from protoplasts of the indica rice breeding line ir72 (oryza sativa l.). | protoplasts were isolated from embryogenic cell suspensions obtained from mature seed derived embryogenic callus of the advanced indica type rice breeding line ir72 available from irri (international rice research institute), manila. culture of protoplasts with the agarose bead type method without nurse culture led to sustained proliferation of protoplast derived clones. a simple culture protocol was developed which stimulated embryogenic development. germination of somatic embryos has so far pr ... | 1992 | 24203129 |
| characterization of a rice (oryza sativa l.) mutant deficient in the heme domain of nitrate reductase. | biochemical and genetical characterization of a rice nitrate reductase (nr)-deficient mutant, m819, which had been isolated as a chlorate-resistant mutant, was carried out. in m819, leaf nadh-nr activity was found to be about 10% of that of the wild-type cv 'norin 8', while nadph-nr activity was higher than that in the wild-type; fmnh2-nr and mv-nr activities were also 10% of those of the wild type; bpb-nr activity was higher than that of the wild type; and xanthine dehydrogenase activity was re ... | 1992 | 24203021 |
| nucleotide sequence of a complementary dna encoding plasma membrane h-atpase from rice (oryza sativa l.). | 1992 | 16668962 | |
| co(2) enhancement of growth and photosynthesis in rice (oryza sativa) : modification by increased ultraviolet-b radiation. | two cultivars of rice (oryza sativa l.) ir-36 and fujiyama-5 were grown at ambient (360 microbars) and elevated co(2) (660 microbars) from germination through reproduction in unshaded greenhouses at the duke university phytotron. growth at elevated co(2) resulted in significant decreases in nighttime respiration and increases in photosynthesis, total biomass, and yield for both cultivars. however, in plants exposed to simultaneous increases in co(2) and ultraviolet-b (uv-b) radiation, co(2) enha ... | 1992 | 16668910 |
| cultivar differences in leaf photosynthesis of rice bred in japan. | the grain yield of rice (oryza sativa l.), as well as of other cereal crops, is limited to a large extent, by the supply of photosynthates produced during grain filling period. in this study, flag leaf photosynthesis (lps) after heading was compared among 32 cultivars bred during the past century in japan, to determine if the improvement of lps has occurred with the breeding advance of high yielding cultivars. measurement of lps was made for 5 consecutive years in the paddy field, on the flag le ... | 1992 | 24408284 |
| nucleotide sequence of the rice (oryza sativa) em protein gene (emp1). | 1992 | 1623185 | |
| nucleotide sequences of two cdna clones encoding different cu/zn-superoxide dismutases expressed in developing rice seed (oryza sativa l.). | 1992 | 1623183 | |
| genomic structure of the gene for copper/zinc-superoxide dismutase in rice. | we have isolated and determined the nucleotide sequence of the structural gene (sodb) coding for one of the copper/zinc-superoxide dismutase (cu/zn-sod) isozymes from rice plants (oryza sativa l.). the sodb gene is split into 8 exons spread about 2 kb of the chromosomal dna, with the coding sequence beginning in the 2nd and ending in the 8th. although none of sodb introns are inserted into similar positions in cu/zn-sod genes from other sources, four of six introns in the protein-coding region a ... | 1992 | 1568478 |
| genome specificity of rdna spacer fragments from oryza sativa l. | the intergenic spacer derived from a cloned rdna unit from a cultivated rice was dissected into several subclones, which were used as probes to analyze sequence homologies between rdna spacers from wild rice belonging to genome types aa, bb, cc, ee, ff, bbcc, and ccdd. this analysis allowed us to detect several regions with different degrees of homology. a series of 250-260 bp repeats is located in the central part of the aa spacer. this sequence cross-hybridizes with the bb, cc, bbcc, and ff ge ... | 1992 | 24202765 |
| regulation of em gene expression in rice : interaction between osmotic stress and abscisic acid. | expression of the em gene was characterized in rice (oryza sativa l.) suspension cultures following exposure of the cultures to various combinations of abscisic acid (aba) and salt. response-saturating concentrations of either aba (50 micromolar) or nacl (0.4 molar) rapidly induced (by 60 minutes) the accumulation of em mrna, with a maximum accumulation occurring 12 to 24 hours after treatment. nacl-induced em expression was accompanied by a doubling of endogenous aba levels as determined by imm ... | 1992 | 16668800 |
| changes in cytosolic glutamine synthetase polypeptide and its mrna in a leaf blade of rice plants during natural senescence. | changes in the levels of cytosolic glutamine synthetase (gs1) and chloroplastic glutamine synthetase (gs2) polypeptides and of their corresponding mrnas have been investigated in segments of the 13th leaf of hydroponically grown rice (oryza sativa l.) plants during natural senescence. the leaf blade on the main stem at early (0 day), middle (15 days), and late (25 days) stages of senescence was harvested and cut into 18 or 19 segments, 2 centimeters in length from the base to the tip. the amount ... | 1992 | 16668795 |
| purification, characterization, and immunological properties of nadh-dependent glutamate synthase from rice cell cultures. | to obtain a monospecific antibody against nadh-dependent glutamate synthase (nadh-gogat; ec 1.4.1.14), the enzyme was purified to homogeneity from cultured rice cells (oryza sativa) with column chromatography using butyl toyopearl 650m, sephacryl s-300, blue sepharose cl-6b, and butyl toyopearl 650s. the specific activity at the final stage of the purification was 9.8 micromoles of glutamate formed per minute per milligram of protein. the yield was 6.1% and purification was 815-fold. analysis by ... | 1992 | 16668794 |
| presence of three rice sucrose synthase genes as revealed by cloning and sequencing of cdna. | by sequencing cdna clones, we have concluded that three distinct sucrose genes are expressed in rice (oryza sativa cv. tainong 67). when the amino acid sequences deduced from these cdnas as well as those of known sucrose synthase are compared, the highest divergence is found in the c-termini. the most suitable dna sequences for use as specific for the mrna derived from these genes have been suggested. | 1992 | 1534703 |
| nondwarf rice seedling bioassay for gibberellins. | the use of nondwarf rice (oryza sativa l.) cultivars treated with uniconazole as test plants for gibberellin (ga) bioassay instead of tan-ginbozu dwarf rice variant was investigated. the sensitivity of six nondwarf rice cultivars to gas was increased substantially by treatment of the seeds with uniconazole. the minimum detectable dose of a ga in the nondwarf cultivars treated with uniconazole was 1- to 1/10-fold of that in the nontreated tanginbozu and 3- to 10-fold of that in uniconazole-treate ... | 1992 | 16668771 |
| two rice (oryza sativa) full-length cdna clones encoding low-molecular-weight heat-shock proteins. | 1992 | 1581572 | |
| genetic diversity and differentiation of indica and japonica rice detected by rflp analysis. | genetic diversity and differentiation in indica and japonica groups of the cultivated rice (oryza sativa l.) were studied by assaying dna restriction fragment length polymorphisms of 12 indica and 14 japonica rice lines digested with three restriction endonucleases. a total of 49 probes were selected to represent the entire rflp map at intervals of 20-30 cm. it was shown that 95 of the 145 possible probe/enzyme combinations, involving 43 probes and all three enzymes, detected restriction fragmen ... | 1992 | 24202597 |
| auxin and growth regulation of rice coleoptile segments : molecular analysis. | auxin-stimulated elongation of apical segments of rice (oryza sativa l. cv arborio) coleoptiles occurring in the first 4 hours of treatment has been studied. cell extension promoted in the first 2 hours by 10 micromolar indole-3-acetic acid (iaa) is specifically auxin-dependent, whereas after 4 hours, elongation also depends on endogenous production of ethylene. similar to other systems, rice coleoptile cell elongation stimulated by auxin requires continuous synthesis of rna and protein. two-dim ... | 1992 | 16668666 |
| effects of anoxia on mitochondrial biogenesis in rice shoots: modification of in organello translation characteristics. | shoots of germinating rice (oryza sativa l.) seedlings are able to grow under anoxia and to withstand long periods of anoxic treatment. mitochondria were purified from aerobically germinated and anaerobically treated rice shoots by differential and isopycnic centrifugation and were found to consist of two subpopulations. the mitochondrial subpopulation of higher density was used for further characterization. ultrastructural studies showed anaerobic mitochondria to be significantly different from ... | 1992 | 16668655 |
| the hydroxyproline-rich glycoprotein gene from oryza sativa. | 1992 | 1536936 | |
| characterization and genetic mapping of a short, highly repeated, interspersed dna sequence from rice (oryza sativa l.). | a short, highly repeated, interspersed dna sequence from rice was characterized using a combination of techniques and genetically mapped to rice chromosomes by restriction fragment length polymorphism (rflp) analysis. a consensus sequence (ggc)n, where n varies from 13-16, for the repeated sequence family was deduced from sequence analysis. southern blot analysis, restriction mapping of repeat element-containing genomic clones, and dna sequence analysis indicated that the repeated sequence is in ... | 1992 | 1347144 |
| characterization and solubilization of β-glucan synthases from cultured rice cells. | two types of β-glucan synthases, gs-i and gs-ii, were found in cultured rice cells (oryza sativa l.). in glycerol density gradient centrifugation, gs-i activity peak co-migrated with a marker enzyme of the golgi membrane, while gs-ii co-migrated with the plasma membrane. analysis of the reaction products of gs-i and gs-ii, suggested that gs-i and gs-ii were mainly β-1,4,- and β-1,3-glucosyltransferases, respectively. gs-i had a higher substrate affinity for udp-glucose than gs-ii, and needed div ... | 1992 | 27320988 |
| mitochondrial genome structure of rice suspension culture from cytoplasmic male-sterile line (a-58cms): reappraisal of the master circle. | the mitochondrial dna (mtdna) from the cultured cells of a cytoplasmic male-sterile line (a-58cms) of rice (oryza sativa) was cloned and its physical map was constructed. there was structural alteration on the mitochondrial genome during the cell culture. detailed restriction analysis of cosmid clones having mtdna fragments suggested either that the master genome has a 100-kb duplication (the genome size becomes 450 kb) or that a master circle is not present in the genome (the net structural com ... | 1992 | 24202508 |
| foliar injury and leaf diffusive resistance of rice and white bean in response to so2 and o3, singly and in combination. | plants of rice (oryza sativa) and white bean (phaseolus vulgaris) were exposed to 524 microg m(-3) so2, 392 microg m(-3) o3 and a mixture of both gases, i.e. 524 microg m(-3) so2 and 392 microg m(-3) o3 to determine the visible foliar injury and leaf diffusive resistance. response of leaf diffusive resistance was measured on upper and lower surfaces of leaves, i.e. the two unifoliate leaves of bean and the first, second and third primary leaves of rice. the difference in the response may be due ... | 1992 | 15092013 |
| green and non-green callus induction from excised rice (oryza sativa) embryos: effects of exogenous plant growth regulators. | calli were induced either from excised rice embryos or from whole seeds in the presence of 1 to 5 mg l-1 naa. after 12 days of culture, calli were induced only from excised rice embryos. we found that excised embryos accumulated naa up to 6 times higher concentration than did whole seeds. in the presence of 1 to 5 mg l-1 naa and 2 to 10 mg l-1 kinetin, chlorophyllous calli were induced from excised rice embryos. chlorophyll contents in the callus tissue increased with increasing kinetin concentr ... | 1992 | 11537753 |
| nucleotide sequence of a high-pi rice (oryza sativa) -amylase gene. | 1992 | 1731997 | |
| molecular characterization of cdna encoding for adenylate kinase of rice (oryza sativa l.). | two types of genes (adk-a, and adk-b) encoding for adenylate kinase (ak, ec 2.7.4.3.) were isolated from the cdna library constructed from poly(a)+ rna of rice (oryza sativa l.). two cdnas were heterogeneous at 5' and 3' ends of non-coding sequences and had possible polyadenylation signals. one of the genes, adk-a, had 1154 bp sequences encoding 241 amino acid residues, while the other type, adk-b, contained 1085 bp sequences encoding for 243 amino acid residues. homology between adk-a and adk-b ... | 1992 | 1302636 |
| characterization of a plant sine, p-sine1, in rice genomes. | we have previously found that a short interspersed element (sine), named p-sine1, is present in the waxy gene of oryza sativa in two copies. here, we cloned five members of p-sine1 located at other loci in o. sativa and determined their nucleotide sequences. these sequences had a t-rich pyrimidine tract at their defined 3' end and were flanked by direct repeats of a sequence of mostly 14-15 bp long like p-sine1s in the waxy gene. the consensus sequence derived from total seven members of p-sine1 ... | 1992 | 1326298 |
| genetic and physical analysis of the rice bacterial blight disease resistance locus, xa21. | nearly isogenic lines (nils) of rice (oryza sativa) differing at a locus conferring resistance to the pathogen xanthomonas oryzae pv. oryzae were surveyed with 123 dna markers and 985 random primers using restriction fragment length plymorphism (rflp) and random amplified polymorphic dna (rapd) analysis. one chromosome 11 marker (rg103) detected polymorphism between the nils that cosegregated with xa21. all other chromosome 11 dna markers tested were monomorphic between the nils, localizing the ... | 1992 | 1362973 |
| isolation and characterization of cdna clones encoding cdc2 homologues from oryza sativa: a functional homologue and cognate variants. | using probes obtained by pcr amplification, we have isolated two cognate rice cdnas (cdc2os-1 and cdc2os-2) encoding structural homologues of the cdc2+/cdc28 (cdc2) protein kinase from a cdna library prepared from cultured rice cells. comparison of the deduced amino acid sequences of cdc2os-1 and cdc2os-2 showed that they are 83% identical. they are 62% identical to cdc28 of saccharomyces cerevisiae and much more similar to the yeast and mammalian p34cdc2 kinases than to rice r2, a cdc2-related ... | 1992 | 1376401 |
| efficiency of the 5'-terminal sequence (omega) of tobacco mosaic virus rna for the initiation of eukaryotic gene translation in escherichia coli. | recent studies have demonstrated that the 5' leader (omega sequence) of tobacco mosaic virus rna has a certain enhancing capacity for translation of mrna in both prokaryotes and eukaryotes. in order to estimate the efficiency of omega to initiate translation of mrna in escherichia coli, in comparison to the shine-dalgarno (s/d) sequence, we have inserted eight different eukaryotic genes into two types of e. coli expression vectors containing one constitutive promoter (p1) but different translati ... | 1992 | 1396694 |
| the wheat mitochondrial genome contains an orf showing sequence homology to the gene encoding the subunit 6 of the nadh-ubiquinone oxidoreductase. | a region of the mitochondrial (mt) dna of wheat was studied because of its homology with other plant mtdnas. sequence analysis revealed an open reading frame encoding a polypeptide of 247 amino acids. comparison of the sequence of the putative polypeptide with the protein sequence data of the swiss-prot library reveals homology with subunit 6 of the nadh-ubiquinone complex of mitochondria from marchantia polymorpha, podospora anserina, chlamydomonas reinhardtii and of chloroplasts from m. polymo ... | 1992 | 1421143 |
| factors influencing agrobacterium-mediated transient expression of gusa in rice. | transient expression of gus in rice (oryza sativa l.) mediated by agrobacterium tumefaciens was characterized using binary vectors containing gusa genes that express minimal (pkiwi105 and pcnl1) or no (p35s-gus-int and pcnl56) gus activity in bacteria. four-day old seedlings obtained from seeds or immature embryos of rice were cut into shoot, root, and seed remnants and inoculated with various strains of a. tumefaciens. transient gus expression events were quantitated histochemically by determin ... | 1992 | 1463839 |
| co-transcription of orf25 and coxiii in rice mitochondria. | southern hybridization analysis using homologous maize probes indicated that orf25 and coxiii are closely linked in the mitochondrial genome of rice (oryza sativa) cultivar ir36. the two coding regions were found on the same 5.1 kb bamhi fragment, and this fragment was cloned, mapped and partially sequenced. using probes for each gene derived from the rice clone, a 2.4 kb dicistronic mrna transcript was found containing both orf25 and coxiii coding regions. multiple 5' ends were identified by pr ... | 1992 | 1617739 |
| identification of an essential tyrosine residue in the catalytic site of a chitinase isolated from zea mays that is selectively modified during inactivation with 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide. | chitinase isolated from zea mays seeds is inactivated by 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (edc) in the absence of exogenous nucleophiles. oligomers of n-acetylglucosamine,n,n',n",n"'-tetra-n-acetylchitotetraose (glcnac4), and to a lesser extent, n,n',n"-tri-n-acetylchitotriose (glcnac3) and n,n'-di-n-acetylchitobiose (glcnac2) provide partial protection against inactivation by the reagent. an examination of the concentration dependence of the protection afforded by glcnac4 revealed ... | 1992 | 1740436 |
| expression of "dehydrin-like" proteins in embryos and seedlings of zizania palustris and oryza sativa during dehydration. | proteins inducible by dehydration and abscisic acid (aba), termed dehydrins or rab (responsive to aba) proteins, have been identified in a number of species and have been suggested to play a role in desiccation tolerance, particularly during seed development. seeds (caryopses) of north american wild rice (zizania palustris var interior [fassett] dore) are tolerant of dehydration to <10% moisture content (fresh weight basis) only under restricted dehydration and rehydration conditions. in compari ... | 1992 | 16668912 |
| increased activity of a cationic peroxidase associated with an incompatible interaction between xanthomonas oryzae pv oryzae and rice (oryza sativa). | rice (oryza sativa l.) cultivar cas 209 carries the gene xa-10 for resistance to race 2 of xanthomonas oryzae pv oryzae, the bacterial blight pathogen. when seedling leaves of cas 209 plants were infiltrated with bacterial cell suspensions of strain pxo86(rif) (race 2, incompatible), total peroxidase activity in extracts from extracellular spaces increased almost threefold between 16 and 24 hours after inoculation. the increase in total peroxidase activity in extracellular extracts was correlate ... | 1992 | 16668969 |
| on the role of abscisic acid and gibberellin in the regulation of growth in rice. | submergence induces rapid elongation of rice coleoptiles (oryza sativa l.) and of deepwater rice internodes. this adaptive feature helps rice to grow out of the water and to survive flooding. earlier, we found that the growth response of submerged deepwater rice plants is mediated by ethylene and gibberellin (ga). ethylene promotes growth, at least in part, by increasing the responsiveness of the internodal tissue to ga. in the present work, we examined the possibility that increased responsiven ... | 1992 | 16668983 |
| elicitation of diterpene biosynthesis in rice (oryza sativa l.) by chitin. | cell-free extracts of uv-irradiated rice (oryza sativa l.) leaves have a much greater capacity for the synthesis from geranylgeranyl pyrophosphate of diterpene hydrocarbons, including the putative precursors of rice phytoalexins, than extracts of unstressed leaves (ka wickham, ca west [1992] arch biochem biophys 293: 320-332). an elicitor bioassay was developed on the basis of these observations in which 6-day-old rice cell suspension cultures were incubated for 40 hours with the substance to be ... | 1992 | 16668985 |
| expression of a maize ubiquitin gene promoter-bar chimeric gene in transgenic rice plants. | we have constructed a chimeric gene consisting of the promoter, first exon, and first intron of a maize ubiquitin gene (ubi-1) and the coding sequence of the bar gene from streptomyces hygroscopicus. this construct was transferred into rice (oryza sativa l.) protoplasts via electroporation, and 10 plants were regenerated from calli that had been selected for resistance to exogenously supplied bialaphos. transgenic plants grown in a greenhouse were resistant to both bialaphos and phosphinothricin ... | 1992 | 16653150 |
| regeneration of herbicide resistant transgenic rice plants following microprojectile-mediated transformation of suspension culture cells. | suspension cells of oryza sativa l. (rice) were transformed, by microprojectile bombardment, with plasmids carrying the coding region of the streptomyces hygroscopicus phosphinothricin acetyl transferase (pat) gene (bar) under the control of either the 5' region of the rice actin 1 gene (act1) or the cauliflower mosaic virus (camv) 35s promoter. subsequently regenerated plants display detectable pat activity and are resistant to basta(tm), a phosphinothricin (ppt)-based herbicide. dna gel blot a ... | 1992 | 24213292 |
| a sulfonylurea herbicide resistance gene from arabidopsis thaliana as a new selectable marker for production of fertile transgenic rice plants. | a mutant acetolactate synthase (als) gene, csr1-1, isolated from sulfonylurea herbicide-resistant arabidopsis thaliana, was placed under control of a cauliflower mosaic virus 35s promoter (35s). rice protoplasts were transformed with the 35s/als chimeric gene and regenerated into fertile transgenic rice (oryza sativa) plants. the 35s/als gene was expressed effectively as demonstrated by northern blot hybridization analysis, and conferred to transformed calli at least 200-fold greater chlorsulfur ... | 1992 | 16653044 |
| transfer of bacterial blight and blast resistance from the tetraploid wild rice oryza minuta to cultivated rice, oryza sativa. | oryza minuta j. s. presl ex c. b. presl is a tetraploid wild rice with resistance to several insects and diseases, including blast (caused by pyricularia grisea) and bacterial blight (caused by xanthomonas oryzae pv. oryzae). to transfer resistance from the wild species into the genome of cultivated rice (oryza sativa l.), backcross progeny (bc1, bc2, and bc3) were produced from interspecific hybrids of o. sativa cv 'ir31917-45-3-2' (2n=24, aa genome) and o. minuta acc. 101141 (2n=48, bbcc genom ... | 1992 | 24203194 |
| effects of 3-methylgibberellin analogs on gibberellin 3β-hydroxylases and plant growth. | six 3-methylgibberellin analogs were synthesized, and their effects on the ga 3β-hydroxylases from immature seeds of phaseolus vulgaris and cucurbita maxima, and/or on the growth of dwarf rice (oryza sativa l. cv. tan-ginbozu) and cucumber (cucumis sativus l. cv. spacemaster) were investigated. 3-methyl-ga5 and 2, 3-didehydro-3-methyl-ga9· inhibited the conversion of [2, 3-(3)h2]ga9 to [2-(3)h]ga4 by ga 3β-hydroxylases from both p. vulgaris and c. maxima at 3 μm and higher. their c/d-ring-rearra ... | 1992 | 27286375 |
| surface structure and properties of plant seed oil bodies. | storage triacylglycerols (tag) in plant seeds are present in small discrete intracellular organelles called oil bodies. an oil body has a matrix of tag, which is surrounded by phospholipids (pl) and alkaline proteins, termed oleosins. oil bodies isolated from mature maize (zea mays) embryos maintained their discreteness, but coalesced after treatment with trypsin but not with phospholipase a2 or c. phospholipase a2 or c exerted its activity on oil bodies only after the exposed portion of oleosin ... | 1992 | 1560029 |
| use of green manure crops in control of hirschmanniella mucronata and h. oryzae in irrigated rice. | four field experiments were conducted to study the effect of sesbania rostrata and aeschynomene afraspera as rotational and green manure crops on the population dynamics of hirschmanniella mucronata and h. oryzae, and subsequent rice yields. the sequential cropping of the legumes with rice controlled both nematode species. in two experiments, yield of rice was related to the nematode population denisites at planting and harvesting of the second rice crop (r(2) = 0.391, p < 0.001, and r(2) = 0.57 ... | 1992 | 19283213 |
| diversification of the rice waxy gene by insertion of mobile dna elements into introns. | the waxy (wx) gene of oryza glaberrima was cloned, and its nucleotide sequence was determined. a waxy mutant of o. glaberrima showing a glutinous phenotype was found to contain a substitution mutation generating a termination codon in the coding region of the wx gene. the wx sequence of o. glaberrima was different from that of oryza sativa by substitutions and insertions/deletions, among which only a few substitutions occurred in several exons not to severely alter the amino acid sequence of the ... | 1991 | 1685658 |
| mitochondrial enzymes in aerobically and anaerobically germinated seedlings of echinochloa and rice. | activities of tricarboxylic acid (tca) cycle enzymes in seedlings of barnyard grass (echinochloa phyllopogon (stapf.) koss) and rice (oryza sativa l.) germinated under aerobic and anaerobic conditions were investigated. in e. phyllopogon, development of tca-cycle enzyme activities during 10 d of anoxia generally paralleled those in air, although at lower rates. after 5 d, e. phyllopogon seedlings germinating under n2 exhibited 50-80% of the activity of seedlings grown in air, except for 2-oxoglu ... | 1991 | 24194241 |
| a lipoxygenase pathway is activated in rice after infection with the rice blast fungus magnaporthe grisea. | lipoxygenase (lox) and lipid hydroperoxide-decomposing activity (lhda) markedly increased in the fifth leaves of rice (oryza sativa cv aichiasahi) after infection with the rice blast fungus, magnaporthe grisea. the increases in the enzyme activities were significantly higher in response to infection with an incompatible strain (race 131) compared with infection with a compatible strain (race 007) of the fungus. using ion-exchange chromatography, we isolated three lox activities (leaf lox-1, -2, ... | 1991 | 16668421 |
| dietary exposure to aflatoxin in benin city, nigeria: a possible public health concern. | a total of 100 samples of various foods comprising of 10 samples each of garri (manihot utilis pohl), beans (phaseolus lunatus), yam flour (dioscorea rotundata), cassava flour (manihot esculentum), melon (citrulus lunatus), onion (allium cepa), rice (oryza sativa), plantain (musa paradisiaca), red pepper (capsicum annuum l., solanaceae) and eggs were screened for the presence of aflatoxins. fifty percent of yam flour; 40% of cassava flour; 30% of garri; 20% of beans and melon and 10% of rice yie ... | 1991 | 1777386 |
| organ-dependent regulation of a plant promoter isolated from rice by 'promoter-trapping' in tobacco. | a vector containing a transcriptionally inactive neomycin phosphotransferase ii gene was used to select promoter sequences from a pool of random genomic dna fragments. this paper describes how one such sequence (p4.7) isolated from oryza sativa acts as a hormonally regulated promoter in nicotiana tabacum. relative expression ratios in leaf, root, midrib, callus, and stem tissue of tobacco plants are 1:5:4:10:17. histochemical assays show that p4.7 activates the uida reporter gene throughout the ... | 1991 | 1844878 |
| a homologue of a nuclear-coded iron-sulfur protein subunit of bovine mitochondrial complex i is encoded in chloroplast genomes. | the chloroplast genomes of marchantia polymorpha, nicotiana tabacum, and oryza sativa contain open reading frames (orfs or potential genes) encoding homologues of some of the subunits of mitochondrial nadh:ubiquinone oxidoreductase (complex i). seven of these subunits (nd1-nd4, nd4l, nd5, and nd6) are products of the mitochondrial genome, and two others (the 49- and 30-kda components of the iron-sulfur protein fraction) are nuclear gene products. these findings have been taken to indicate the pr ... | 1991 | 1901022 |
| cloning and characterization of repetitive dna sequences from genomes of oryza minuta and oryza australiensis. | the value of genome-specific repetitive dna sequences for use as molecular markers in studying genome differentiation was investigated. five repetitive dna sequences from wild species of rice were cloned. four of the clones, pom1, pom4, poma536, and pompb10, were isolated from oryza minuta accession 101141 (bbcc genomes), and one clone, poa237, was isolated from oryza australiensis accession 100882 (ee genome). southern blot hybridization to different rice genomes showed strong hybridization of ... | 1991 | 1955157 |
| magnaporthe grisea genes for pathogenicity and virulence identified through a series of backcrosses. | we have identified genes for pathogenicity toward rice (oryza sativa) and genes for virulence toward specific rice cultivars in the plant pathogenic fungus magnaporthe grisea. a genetic cross was conducted between the weeping lovegrass (eragrostis curvula) pathogen 4091-5-8, a highly fertile, hermaphroditic laboratory strain, and the rice pathogen o-135, a poorly fertile, female-sterile field isolate that infects weeping lovegrass as well as rice. a six-generation backcrossing scheme was then un ... | 1991 | 2016048 |
| isolation and characterization of a rice gene encoding a basic chitinase. | chitinase, which catalyzes the hydrolysis of the beta-1,4-n-acetyl-d-glucosamine linkages of the fungal cell wall polymer chitin, is involved in inducible defenses of plants. a basic chitinase genomic sequence was isolated from a rice (oryza sativa l.) genomic library using a bean chitinase gene fragment as a probe. the complete nucleotide sequence of the rice chitinase rch10 gene was determined, and shown to contain an open reading frame with no introns, encoding a polypeptide of 336 amino acid ... | 1991 | 2034221 |
| the isolation and characterisation of plant sequences homologous to human hypervariable minisatellites. | we have isolated dna probes, homologous to the human hypervariable minisatellite sequence 33.15, from the genome of rice (oryza sativa). these probes are capable of producing a multilocus rice dna fingerprint. the rice sequence has a tandem repeating structure based on a 12 bp gc-rich repeat which shows homology to its human counterpart. this probe detects up to 30 loci which are at a number of unlinked chromosomal sites. the gc-rich sequence is invariably associated with an open reading frame ( ... | 1991 | 1651259 |
| transgenic rice cell lines and plants: expression of transferred chimeric genes. | cell suspension-derived rice (oryza sativa l.) protoplasts were transformed by direct gene uptake. peg-mediated transformation was more efficient than electroporation. plasmid dna containing a hygromycin phosphotransferase (hpt) gene (which confers hygromycin resistance) driven by the camv 35s promoter and a beta-d-glucuronidase (gus) gene under control of the 1', 2' double promoter of the mannopine synthase (mas) locus of agrobacterium tumefaciens was introduced into rice protoplasts. southern ... | 1991 | 1713479 |
| a novel ras-related rgp1 gene encoding a gtp-binding protein has reduced expression in 5-azacytidine-induced dwarf rice. | exposure of normal, tall rice (oryza sativa) seedlings to 5-azacytidine, a powerful inhibitor of dna methylation in vivo, induced both demethylation of genomic dna and dwarf plants. genes that had been affected by treatment were identified by differential screening of a cdna library, and a ras-related gene, rgp1, was subsequently isolated. the cdna of rgp1 was found to encode a deduced protein sequence of 226 amino acids with a relative molecular mass of 24850, which was most closely related to ... | 1991 | 1715972 |
| purification and characterization of rice peroxidases. | four peroxidase components, named rp-2, 4, 6, and 7, were isolated from rice (oryza sativa l.) green leaves. isoelectric focusing indicated that each preparation was homogeneous. the molecular weights of rp-2, 4, 6, and 7 estimated by sds-page were 48,000, 48,000, 40,000, and 39,500, and their isoelectric points were 5.4, 8.1, 9.3, and 9.2, respectively. the activity of every preparation was maximum around ph 5.0. antisera against these purified enzymes were raised in rabbits. ouchterlony double ... | 1991 | 1368754 |
| diallel analysis of tiller number at different growth stages in rice (oryza sativa l.). | the genetics of tiller number at different development stages in rice was studied in a 6 x 6 diallel cross set involving six indica rice varieties in which a large difference of phenotypes existed. estimates of genetic parameters following hayman's method showed significant additive and nonadditive gene action, and the latter appeared to be solely due to dominance. high tillering ability was inherited as a partial dominant character conditioned by two or more blocks of genes, and var 'h1459' had ... | 1991 | 24202365 |
| structural homology between semidwarfism-related proteins and glutelin seed protein in rice (oryza sativa l.). | two semidwarfism-related proteins, srp-1 and srp-2, were detected as major spots in a long-culm rice cultivar, norin 29 and its semidwarf near-isogenic line, sc-tn1, respectively, by two-dimensional gel electrophoresis (2d-page). the testcross showed that srp-1 and srp-2 are controlled by codominant alleles, srp-1 and srp-2, respectively, at a single locus srp. this locus was considered to be closely linked with the semidwarfing gene locus sd-1. srp-1 and srp-2 were separated by 2d-page, electro ... | 1991 | 24202351 |
| genetic analysis of endosperm mutants in rice oryza sativa l. | sugary, shrunken, floury, white core, amylose extender and dull mutants induced in japonica varieties were used in this study. the results of an allelic analysis conducted in japonica background indicated that the two sugary mutants 82gf and em5 are allelic. the two amylose extender mutants 2064 and em16 are also allelic. the opaque mutant esd7-3(0) and floury mutants 2047, em17 and em28 are allelic as well and have the flo-1 gene. the three white core mutants em3, em24 and em66 were found to be ... | 1991 | 24202350 |
| photosynthetic characteristics of rice leaves aged under different irradiances from full expansion through senescence. | effects of irradiance on photosynthetic characteristics were examined in senescent leaves of rice (oryza sativa l.). two irradiance treatments (100 and 20% natural sunlight) were imposed after the full expansion of the 13th leaf through senescence. the photosynthetic rate was measured as a function of intercellular co(2) pressure with a gas-exchange system. the amounts of cytochrome f, coupling factor 1, ribulose-1,5-bisphosphate carboxylase/oxygenase (rubisco), and chlorophyll were determined. ... | 1991 | 16668545 |
| field uniformity of the japonica rice region of taiwan as estimated by relative genetic contribution. | despite the concerns for genetic vulnerability that were raised in the 1970s, the field uniformity of the japonica rice (oryza sativa l.) region in taiwan has increased since 1980 with over 82% of the cultivated areas being covered by as few as three varieties and over half of this hectarage by a single variety. japanese plant introductions are the major ancestral contributors of genetic constituents for varieties released in taiwan. the main constitution of the genetic base present in the field ... | 1991 | 24202264 |