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murine pulmonary infection with listeria monocytogenes: differential susceptibility of balb/c, c57bl/6 and dba/2 mice.murine listeriosis is a paradigm to understand host pathogen interactions. airway infections with listeria monocytogenes, although representing a serious problem in early onset neonatal listeriosis, has not been investigated in detail in animal models so far. here, the susceptibility of balb/c, dba/2 and c57bl/6 mice towards an intratracheal (i.t.) infection with virulent l. monocytogenes egde and the attenuated variant l. monocytogenes egd hlyw491a(perl3-cmvgfp) is reported. the course of infec ...200515820148
multistate outbreak of listeria monocytogenes infection linked to delicatessen turkey meat.despite a decreasing incidence of listeriosis in the united states, molecular subtyping has increased the number of recognized outbreaks. in september 2000, the new york city department of health identified a cluster of infections caused by listeria monocytogenes isolates with identical molecular subtypes by pulsed-field gel electrophoresis (pfge) and ribotyping.200515824987
gastroenteritis due to listeria monocytogenes.it has been known for a long time that many patients experience diarrhea antecedent to the development of bacteremia or meningoencephalitis due to listeria monocytogenes, but it was only recently that convincing evidence was obtained that this organism can cause acute, self-limited, febrile gastroenteritis in healthy persons. at least 7 outbreaks of foodborne gastroenteritis due to l. monocytogenes have been reported. illness typically occurs 24 h after ingestion of a large inoculum of bacteria ...200515825036
mode of action of piscicocin cs526 produced by carnobacterium piscicola cs526.piscicocin cs526 is a unique class iia bacteriocin produced by carnobacterium piscicola cs526. the mode of action against the sensitive strain listeria monocytogenes iid581 was evaluated.200515836484
characterization of a bacteriocin produced by enterococcus faecium gm-1 isolated from an infant.to partially characterize the bacteriocin produced by the gm-1 strain of enterococcus faecium, isolated from the faeces of a newborn human infant.200515836487
inactivation of escherichia coli, listeria monocytogenes, pseudomonas aeruginosa and staphylococcus aureus on stainless steel and glass surfaces by neutral electrolysed water.to ascertain the efficacy of neutral electrolysed water (new) in reducing escherichia coli, pseudomonas aeruginosa, staphylococcus aureus and listeria monocytogenes on glass and stainless steel surfaces. its effectiveness for that purpose is compared with that of a sodium hypochlorite (naclo) solution with similar ph, oxidation-reduction potential (orp) and active chlorine content.200515836736
specific molecular detection of carnobacterium piscicola sf668 in cold smoked salmon.to establish a rapid and reliable multiplex pcr (mpcr)-based method allowing specific identification of carnobacterium piscicola sf668 during storage of cold smoked salmon (css).200515836740
the listeria monocytogenes prfap2 promoter is regulated by sigma b in a growth phase dependent manner.listeria monocytogenes prfa, encoding positive regulatory factor a, is transcribed from three promoters (prfap1, prfap2, and pplca). the prfap2 promoter was previously proposed to be sigma b (sigma(b))-dependent. this hypothesis was tested by creating prfa promoter-gus transcriptional fusions in both l. monocytogenes wild-type (wt) and deltasigb backgrounds and then measuring (i) beta-glucuronidase (gus) activities; (ii) gus mrna transcript levels; and (iii) the presence or absence of gus in cel ...200515837390
comparative proteome analysis of secretory proteins from pathogenic and nonpathogenic listeria species.extracellular proteins of bacterial pathogens play a crucial role in the infection of the host. here we present the first comprehensive validation of the secretory subproteome of the gram positive pathogen listeria monocytogenes using predictive bioinformatic and experimental proteomic approaches. the previous original signal peptide (sp) prediction (glaser et al., science 2001, 294, 849-852) has been greatly improved by an in-depth analysis using seven different bioinformatic tools. subsequent ...200515838904
bacterial delivery of functional messenger rna to mammalian cells.the limited access to the nuclear compartment may constitute one of the major barriers after bacteria-mediated expression plasmid dna delivery to eukaryotic cells. alternatively, a self-destructing listeria monocytogenes strain was used to release translation-competent mrna directly into the cytosol of epithelial cells, macrophages and human dendritic cells. enhanced green fluorescent protein (egfp)-encoding mrna, adapted for translation in mammalian cells by linking an ires element to the 5'-en ...200515839900
reducing the stimulation of cd8+ t cells during infection with intracellular bacteria promotes differentiation primarily into a central (cd62lhighcd44high) subset.during infection with lymphocytic choriomeningitis virus, cd8(+) t cells differentiate rapidly into effectors (cd62l(low)cd44(high)) that differentiate further into the central memory phenotype (cd62l(high)cd44(high)) gradually. to evaluate whether this cd8(+) t cell differentiation program operates in all infection models, we evaluated cd8(+) t cell differentiation during infection of mice with recombinant intracellular bacteria, listeria monocytogenes (lm) and mycobacterium bovis (bcg), expres ...200515843531
impact of antimicrobial ingredients and irradiation on the survival of listeria monocytogenes and the quality of ready-to-eat turkey ham.irradiation is an effective technology in eliminating listeria monocytogenes, but it induces quality changes in meat products at or above specific radiation doses. to minimize irradiation-induced quality changes, only low irradiation doses are recommended. however, low-dose irradiation provides a chance for some pathogens to survive and proliferate during prolonged storage. to solve this problem, antimicrobial ingredients [2% sodium lactate (sl), 0.1% sodium diacetate (sda), 0.1% potassium benzo ...200515844819
listeria monocytogenes skin infection with cerebritis and haemophagocytosis syndrome in a bone marrow transplant recipient.in this report, we describe unusual and unreported manifestations of listeria monocytogenes infection in a bone marrow transplant recipient, including cutaneous infection with an hamophagocytosis syndrome and cerebritis. l. monocytogenes occurred despite a broad spectrum antibiotherapy. l. monocytogenes was isolated from a skin biopsy. outcome was favorable with amoxicillin and gentamicin therapy. l. monocytogenes infection should be suspected in patients with cerebritis despite large spectrum a ...200515845437
molecular cloning and characterization of three beta-defensins from canine testes.mammalian beta-defensins are small cationic peptides possessing broad antimicrobial and physiological activities. because dogs are particularly resilient to sexually transmitted diseases, it has been proposed that their antimicrobial peptide repertoire might provide insight into novel antimicrobial therapeutics and treatment regimens. to investigate this proposal, we cloned the full-length cdna of three canine beta-defensin isoforms (cbd-1, -2, and -3) from canine testicular tissues. their predi ...200515845463
synthetic toll-like receptor 4 agonists stimulate innate resistance to infectious challenge.a compound family of synthetic lipid a mimetics (termed the aminoalkyl glucosaminide phosphates [agps]) was evaluated in murine infectious disease models of protection against challenge with listeria monocytogenes and influenza virus. for the listeria model, intravenous administration of agps was followed by intravenous bacterial challenge 24 h later. spleens were harvested 2 days postchallenge for the enumeration of cfu. for the influenza virus model, mice were challenged with virus via the int ...200515845512
construction and characterization of listeria monocytogenes mutants with in-frame deletions in the response regulator genes identified in the genome sequence.two-component systems are widely distributed in prokaryotes where they control gene expression in response to diverse stimuli. to study the role of the sixteen putative two-component systems of listeria monocytogenes systematically, in frame deletions were introduced into 15 out of the 16 response regulator genes and the resulting mutants were characterized. with one exception the deletion of the individual response regulator genes has only minor effects on in vitro and in vivo growth of the bac ...200515845524
brain stem encephalitis in listeriosis.serious infection with the bacterium l. monocytogenes mainly manifests as sepsis and/or meningitis. a particular entity is listeria brain stem encephalitis, which is characterized by progressive brain stem deficits. the condition is fatal unless early treated. the purpose of the present study was to assess the incidence of brain stem encephalitis in a population-based listeriosis material. medical records from 212 of the 240 patients with serious listeriosis reported in norway from 1977 to 2000, ...200515849051
differential requirements for the chemokine receptor ccr7 in t cell activation during listeria monocytogenes infection.effective priming of t cell responses depends on cognate interactions between naive t cells and professional antigen-presenting cells (apcs). this contact is the result of highly coordinated migration processes, in which the chemokine receptor ccr7 and its ligands, ccl19 and ccl21, play a central role. we used the murine listeria monocytogenes infection model to characterize the role of the ccr7/ccr7 ligand system in the generation of t cell responses during bacterial infection. we demonstrate t ...200515851484
thermal resistance of salmonella enterica serotypes, listeria monocytogenes, and staphylococcus aureus in high solids liquid egg mixes.decimal reduction times (d-values) were determined for salmonella enterica serotypes, listeria monocytogenes, and staphylococcus aureus in two high solids egg mixes designated a and b (water activity [a(w)] = 0.76 and 0.82; solids = 53.12 and 52.63%; ph = 5.09 and 5.29; viscosity = 183 and 119 centipoise/s, respectively) using a low-volume (0.06 ml) sealed glass capillary tube procedure. for salmonella, d-values ranged from 0.035 (70 degrees c) to 0.193 min (64 degrees c) in product a and from 0 ...200515830659
development of a surface plasmon resonance-based immunoassay for listeria monocytogenes.a polyclonal antibody was produced against internalin b (inlb)-enriched extract and used to develop an inhibition assay to detect listeria monocytogenes cells in solution using surface plasmon resonance. the gene sequence encoding for the inlb protein was cloned into a qiagen pqe-60 vector, expressed in escherichia coli, and purified by immobilized metal affinity chromatography. protein g-purified anti-inlb-enriched extract polyclonal antibody was incubated with various concentrations of l. mono ...200515830663
predicting heat inactivation of listeria monocytogenes under nonisothermal treatments.the aim of this study was to find a model that accurately predicts the heat inactivation of listeria monocytogenes (atcc 15313) at constantly rising heating rates (0.5 to 9 degrees c/min) in media of different ph values (4.0 to 7.4). survival curves of l. monocytogenes obtained under isothermal treatments at any temperature were nearly linear. estimations of survival curves under nonisothermal treatments obtained from heat resistance parameters of isothermal treatments adequately fit experimenta ...200515830664
inactivation of barotolerant listeria monocytogenes in sausage by combination of high-pressure processing and food-grade additives.food-grade additives were used to enhance the efficacy of high-pressure processing (hpp) against barotolerant listeria monocytogenes. three strains of l. monocytogenes (scott a, osy-8578, and osy-328) were compared for their sensitivity to hpp, nisin, tert-butylhydroquinone (tbhq), and their combination. inactivation of these strains was evaluated in 0.2 m sodium phosphate buffer (ph 7.0) and commercially sterile sausage. a cell suspension of l. monocytogenes in buffer (10(9) cfu/ml) was treated ...200515830665
monochloramine versus sodium hypochlorite as antimicrobial agents for reducing populations of bacteria on broiler chicken carcasses.studies were conducted to compare the effect of sodium hypochlorite (sh) versus monochloramine (mon) on bacterial populations associated with broiler chicken carcasses. in study 1, nominal populations (6.5 to 7.5 log cfu) of escherichia coli, listeria monocytogenes, pseudomonas fluorescens, salmonella serovars, shewanella putrefaciens, and staphylococcus aureus were exposed to sterilized chiller water (controls) or sterilized chiller water containing 50 ppm sh or mon. sh at 50 ppm eliminated all ...200515830667
fate of listeria monocytogenes inoculated onto the surface of model turkey frankfurter pieces treated with zein coatings containing nisin, sodium diacetate, and sodium lactate at 4 degrees c.the antimicrobial effects of zein coatings containing nisin, sodium lactate, and sodium diacetate against listeria monocytogenes on turkey frankfurters at 4 degrees c were determined. our objectives were to determine whether zein, nisin, lactate, and diacetate alone or in combination could control the growth of l. monocytogenes on full-fat turkey frankfurters at 4 degrees c and to determine whether lactate or diacetate had any synergistic effect on the activity of nisin. turkey frankfurter piece ...200515830684
effectiveness of trisodium phosphate treatment against pathogenic and spoilage bacteria on poultry during refrigerated storage.to determine the efficacy of trisodium phosphate (tsp) against pathogenic and spoilage bacteria on poultry and to assess the influence of the bacterial combination of inoculum on tsp effect, chicken legs were coinoculated with similar concentrations of a pathogenic (salmonella enterica ser. enteritidis or listeria monocytogenes) and a spoilage (pseudomonas fluorescens or brochothrix thermosphacta) bacteria. samples were dipped in tsp (12%, 15 min) or were not treated (control). microbiological a ...200515830686
the role of sigmab in the stress response of gram-positive bacteria -- targets for food preservation and safety.the alternative sigma factor sigmab modulates the stress response of several gram-positive bacteria, including bacillus subtilis and the food-borne human pathogens bacillus cereus, listeria monocytogenes and staphylococcus aureus. in all these bacteria, sigmab is responsible for the transcription of genes that can confer stress resistance to the vegetative cell. recent findings indicate that sigmab also plays an important role in antibiotic resistance, pathogenesis and cellular differentiation p ...200515831390
[meningitis caused by listeria monocytogenes in a patient with myasthenia gravis being treated with tacrolimus]. 200515795876
differential cytosolic delivery and presentation of antigen by listeriolysin o-liposomes to macrophages and dendritic cells.delivery of antigenic protein to the cytosol of antigen-presenting cells (apcs), such as macrophages (mphi) and dendritic cells (dcs), is required for an efficient cd8 t-cell-mediated immune response. we have previously shown that co-encapsulation of antigenic protein inside ph-sensitive liposomes with listeriolysin o (llo), a pore-forming protein of listeria monocytogenes, generates efficient major histocompatibility complex class i (mhc i)-restricted immune responses both in vitro and in vivo. ...200515804180
super paramagnetic clustering of protein sequences.detection of sequence homologues represents a challenging task that is important for the discovery of protein families and the reliable application of automatic annotation methods. the presence of domains in protein families of diverse function, inhomogeneity and different sizes of protein families create considerable difficulties for the application of published clustering methods.200515804359
the metalloprotease of listeria monocytogenes controls cell wall translocation of the broad-range phospholipase c.listeria monocytogenes is a gram-positive bacterial pathogen that multiplies in the cytosol of host cells and spreads directly from cell to cell. during cell-to-cell spread, bacteria become temporarily confined to secondary vacuoles. the broad-range phospholipase c (pc-plc) of l. monocytogenes contributes to bacterial escape from secondary vacuoles. pc-plc requires cleavage of an n-terminal propeptide for activation, and mpl, a metalloprotease of listeria, is involved in the proteolytic activati ...200515805506
enhanced synthesis of internalin a in aro mutants of listeria monocytogenes indicates posttranscriptional control of the inlab mrna.listeria monocytogenes mutants with deletions in aroa, arob, or aroe exhibited strong posttranscriptional upregulation of internalin a (inla) and inlb synthesis, which resulted in a more-than-10-fold increase in inla-mediated internalization by epithelial caco-2 cells and a 4-fold increase in inlb-mediated internalization by microvascular endothelial cells (human brain microvascular endothelial cells) compared to the wild-type strain. the increase in inla and inlb production was not due to enhan ...200515805530
[investigation of electrophysical properties of listeria monocytogenes cells during the interaction with monoclonal antibodies].an electrooptical approach was used in studies of listeria monocytogenes-antibody binding. an electrooptical analyzer, which has been developed at the state research center for applied microbiology (obolensk, russia), was used as a basic instrument for electrooptical measurements. the analyzer consists of the following modules: a sample preparation module, a mixer, an ac field generator, an eo-flow cell, a microcontroller for transfer of liquid, a thermal system, an operator interface, and an im ...200515856991
influence of bacteriocin-like substance, generation times, and genetic profiles of listeria innocua on the isolation of listeria monocytogenes.inhibition of isolation of listeria monocytogenes by bacteriocin-like substance (bls)-producing listeria innocua after enrichment culture was investigated. when 26 l. monocytogenes strains were examined in combination with eight l. innocua strains using the spot on lawn method, 52/208 (25.0%) combinations showed the growth inhibition of l. monocytogenes. when two listeria species were cultured simultaneously in selective enrichment broth, inhibition of isolation of l. monocytogenes was observed ...200515857657
evaluation of a chromogenic medium for identification and differentiation of listeria monocytogenes in selected foods.listeria monocytogenes continues to be a threat to food safety in the united states despite a "zero tolerance" policy. when listeria species are identified by standard cultural methods, confirmation of l. monocytogenes takes days to complete. rapid'l.mono agar, developed by bio-rad laboratories, is a chromogenic medium that differentiates l. monocytogenes from other species of listeria by a simple color change reaction. differentiation is based on the specific detection of phosphatidylinositol p ...200515859079
activity of three {beta}-lactams (ertapenem, meropenem and ampicillin) against intraphagocytic listeria monocytogenes and staphylococcus aureus.assessment of the activity of three beta-lactams [ertapenem (a carbapenem with a prolonged half-life), meropenem and ampicillin] against intraphagocytic listeria monocytogenes and staphylococcus aureus.200515860552
fate of listeria monocytogenes in experimentally contaminated french sausages.listeria monocytogenes has been recognized as one of the most important foodborne pathogens dealt with by the food. the bacterium has been found in every part along the pork processing industry from the slaughterhouse to the cutting room and the delicatessen factories. during the fermentation and drying of sausages, l. monocytogenes tends to decrease substantially. however, despite the various hurdles in the dry sausage manufacturing process, l. monocytogenes is able to survive and is detected i ...200515862881
growth of listeria monocytogenes on iceberg lettuce and solid media.the growth of pathogenic bacterium listeria monocytogenes on fresh-cut iceberg lettuce under constant temperatures was modelled in order to investigate microbial safety during distribution of this vegetable. we examined the effects of several incubation temperatures, ranging from 5 to 25 degrees c, on bacterial growth. these data were fitted to the baranyi model and the curves showed a high correlation coefficient at all temperature (r2 > 0.95). in addition, the native bacterial flora of the let ...200515862883
pesticides as a source of microbial contamination of salad vegetables.ten commercially available pesticides (insecticides, herbicides and fungicides), used during the production of vegetable produce, were examined as potential sources of microbial contaminants. as purchased, none of the pesticides showed the presence of viable microorganisms (< 5 cfu/ml). using an agar plate diffusion assay, they did not inhibit a range of bacteria of spoilage and public health significance on vegetable produce. after reconstitution in sterile water to their recommended concentrat ...200515862885
beyond allergen avoidance: update on developing therapies for peanut allergy.food allergy has emerged as a significant health problem. peanut allergy is a major cause of food-induced fatal and near fatal anaphylactic reactions, and the incidence in children is increasing. attempts to manage peanut allergy by strict avoidance are often unsuccessful. the purpose of this review is to highlight the most promising novel approaches for treating peanut allergy beyond allergen avoidance.200515864090
[neurolisteriosis with acute myelitis].listeriosis is a bacterial infection with listeria monocytogenes mostly affecting immunocompromised patients. in every fourth case, the cns is involved, usually as meningoencephalitis. this case report of an immunocompetent woman represents the first one of neurolisteriosis initially presenting as cervical myelitis and progressing to supratentorial areas of the brain. diagnosis was based on universal polymerase chain reaction from a cortical brain biopsy, followed by sequencing of the amplified ...200515864516
dietary (n-3) polyunsaturated fatty acids do not affect the in vivo development and function of listeria-specific cd4+ and cd8+ effector and memory/effector t cells in mice.we previously reported that in a mouse model, a diet high in (n-3) pufa diminishes host survival following an infection from listeria monocytogenes, a gram-positive bacterial pathogen. in this study we investigated the impact of (n-3) pufa on the adaptive immune response to l. monocytogenes. balb/c mice were fed experimental diets either devoid of or rich in (n-3) pufa from fish oil for 4 wk and then infected with 10(6) acta-deficient l. monocytogenes. at 7 and 35 d postchallenge, effector and m ...200515867296
potential role of diploscapter sp. strain lkc25, a bacterivorous nematode from soil, as a vector of food-borne pathogenic bacteria to preharvest fruits and vegetables.diploscapter, a thermotolerant, free-living soil bacterial-feeding nematode commonly found in compost, sewage, and agricultural soil in the united states, was studied to determine its potential role as a vehicle of salmonella enterica serotype poona, enterohemorrhagic escherichia coli o157:h7, and listeria monocytogenes in contaminating preharvest fruits and vegetables. the ability of diploscapter sp. strain lkc25 to survive on agar media, in cow manure, and in composted turkey manure and to be ...200515870330
growth of listeria monocytogenes in the guinea pig placenta and role of cell-to-cell spread in fetal infection.listeria monocytogenes causes foodborne outbreaks that lead to infection in human and other mammalian fetuses. to elucidate the molecular and cellular mechanisms involved in transplacental transmission, we characterized placental-fetal infection in pregnant guinea pigs inoculated with wild-type (wt) or mutant l. monocytogenes strains. the wt strain increased in number in the placenta by >1000-fold during the first 24 h after inoculation--an increase that was unparalleled in other maternal organs ...200515871123
microbiological aspects of the investigation that traced the 1998 outbreak of listeriosis in the united states to contaminated hot dogs and establishment of molecular subtyping-based surveillance for listeria monocytogenes in the pulsenet network.a multistate outbreak of listeriosis occurred in the united states in 1998 with illness onset dates between august and december. the outbreak caused illness in 108 persons residing in 24 states and caused 14 deaths and four miscarriages or stillbirths. this outbreak was detected by public health officials in tennessee and new york who observed significant increases over expected listeriosis cases in their states. subsequently, the centers for disease control and prevention (cdc) began laboratory ...200515872265
listeria-associated arthritis in a patient undergoing etanercept therapy: case report and review of the literature.listeriosis can be a cause of infectious arthritis. here, we present a case of articular listeriosis in a patient with rheumatoid arthritis receiving treatment with etanercept, a tumor necrosis factor antagonist. we review the literature of articular listeriosis and discuss the role of tumor necrosis factor blockade in precipitating listeriosis.200515872306
listeria meningitis in transplant recipients.meningitis is a rare complication following organ and stem-cell transplantation and can be caused by a variety of microorganisms.200515875764
tracing listeria monocytogenes isolates from cold-smoked salmon and its processing environment in iceland using pulsed-field gel electrophoresis.listeria spp. and listeria monocytogenes contamination of cold-smoked salmon (n=125) and its processing environment (n=522) were evaluated during surveys conducted in 1997-1998 and 2001 as well as in samples of final products analysed in 2001. the overall frequencies of listeria spp. and l. monocytogenes in samples from all sources were 15.1% and 11.3%, respectively, but the incidence of l. monocytogenes in cold-smoked salmon final products was only 4%. a total of 201 l. monocytogenes isolates w ...200515878405
effect of prior growth temperature, type of enrichment medium, and temperature and time of storage on recovery of listeria monocytogenes following high pressure processing of milk.a five-isolate cocktail of listeria monocytogenes (10(3) cfu/ml in skim or whole raw milk) was subjected to 450 mpa for 900 s or 600 mpa for 90 s. the effects of prior growth temperature, type of milk (skim vs. whole), type of recovery-enrichment media (optimized penn state university [opsu] broth, listeria enrichment broth [leb], buffered leb [bleb], modified bleb [mbleb], and milk), storage temperature and storage time on the recovery of l. monocytogenes were examined. optimized psu broth sign ...200515878406
rapid clearance of a recombinant salmonella vaccine carrier prevents enhanced antigen-specific cd8 t-cell responses after oral boost immunizations.the type iii secretion system of salmonella enterica serovar typhimurium can be used to target heterologous antigens directly into the cytosol of antigen-presenting cells. our laboratory has previously reported that the single oral immunization of mice with a recombinant salmonella strain expressing the translocated yersinia outer protein e fused to the immunodominant antigen p60 from listeria monocytogenes results in the efficient induction of p60-specific cd8 t cells and confers protection aga ...200515878680
internalin-expressing lactococcus lactis is able to invade small intestine of guinea pigs and deliver dna into mammalian epithelial cells.the use of the food-grade bacterium lactococcus lactis as antigen delivery vehicle at the mucosal level is an attractive vaccination strategy intensively explored during the last decade. in this study, we developed l. lactis strains which could be used as a dna delivery vector to combine both advantages of mucosal delivery and of dna vaccination. to render lactococci capable of invading epithelial cells, the listeria monocytogenes inla gene was cloned and expressed in l. lactis under transcripti ...200515878681
cutting edge: re-evaluating the in vivo cytokine responses of cd8+ t cells during primary and secondary viral infections.virus-specific cd8(+) t cells produce ifn-gamma after ag contact and, in the absence of this cytokine, the host often cannot eradicate infection. however, our ability to identify cells that are actively expressing this critical effector function in vivo is limited, because the protein is rapidly secreted. in this study, we describe a simple approach that circumvents the need for ex vivo ag stimulation and allows the enumeration of cd8(+) t cells that are actively synthesizing ifn-gamma in vivo d ...200515879085
large-scale quantitative analysis of sources of variation in the actin polymerization-based movement of listeria monocytogenes.during the actin polymerization-based movement of listeria monocytogenes, individual bacteria are rapidly propelled through the host cell cytoplasm by the growth of a filamentous actin tail. the rate of propulsion varies significantly among individuals and over time. to study this variation, we used a high-throughput tracking technique to record the movement of a large number (approximately 7900) of bacteria in xenopus frog egg extract. most bacteria (70%) appeared to maintain an individual char ...200515879472
rat hepatocyte invasion by listeria monocytogenes and analysis of tnf-alpha role in apoptosis.listeria monocytogenes, etiological agent of severe human foodborne infection, uses sophisticated mechanisms of entry into host cytoplasm and manipulation of the cellular cytoskeleton, resulting in cell death. the host cells and bacteria interaction may result in cytokine production as tumor necrosis factor (tnf) alpha. hepatocytes have potential to produce pro-inflammatory cytokines as tnf-alpha when invaded by bacteria. in the present work we showed the behavior of hepatocytes invaded by l. mo ...200515880217
[influence of gaseous metabolites of soil bacteria on the multiplication of listeria monocytogenes and yersinia pseudotuberculosis].the influence of gaseous metabolites of saprophytic soil bacteria on the growth and multiplication of l. monocytogenes and y. pseudotuberculosis was studied. the study revealed that all cultures under study exhibited selectively both inhibiting and stimulating action on the multiplication of test cultures. bacteria of the genera pseudomonas and acinetobacter showed the highest inhibiting activitywith respect to the test cultures. volatile metabolites of bacteria of the genus aeromonas showed the ...200515881930
understanding how listeria monocytogenes targets and crosses host barriers.human listeriosis is caused by the gram-positive bacterium listeria monocytogenes. in humans, this pathogen has the ability to cross the intestinal, placental and blood-brain barriers, leading to gastroenteritis, maternofetal infections and meningoencephalitis, respectively. the entry of l. monocytogenes into cultured human epithelial cells is mediated by the interaction of an l. monocytogenes surface protein, internalin, with its human receptor, e-cadherin. the internalin-e-cadherin interaction ...200515882192
the listeria protein internalin b mimics hepatocyte growth factor-induced receptor trafficking.increased hepatocyte growth factor receptor (hgfr) signaling correlates closely with neoplastic invasion and metastatic potential of many human cancers. hepatocyte growth factor receptor signaling is initiated by binding the physiological ligand hgf or the internalin b (inlb) protein of listeria monocytogenes. subsequent degradation of endocytosed hgfr terminates receptor signaling. previously reported discrepancies in inlb and hgf-induced hgfr signaling could reflect differences in receptor int ...200515882443
apoptotic death of listeria monocytogenes-infected human macrophages induced by lactoferricin b, a bovine lactoferrin-derived peptide.listeria monocytogenes, an intracellular facultative food-borne pathogen, was reported to induce apoptosis in vitro and in vivo in a variety of cell types with the exception of murine macrophages. these cells represent the predominant compartment of bacterial multiplication and die as a result of necrosis. in this study we showed that human non-activated and ifn-gamma-activated macrophagic-like (thp-1) cells infected with l. monocytogenes, mainly die by necrosis rather than by an apoptotic proce ...200515888254
diagnosis of partially treated culture-negative bacterial meningitis using 16s rrna universal primers and restriction endonuclease digestion.cerebrospinal fluid (csf) obtained from patients with partially treated and culture-negative meningitis was subjected to pcr using 16s rdna universal primers followed by restriction endonuclease digestion. in all, 43 patients and 7 controls were enrolled in this study. twenty-one meningitic samples were positive by pcr. mycobacterium tuberculosis was the causative agent in seven cases followed by haemophilus influenzae (four), streptococcus pneumoniae (two), listeria monocytogenes (one), escheri ...200515888461
dendritic cells maximize the memory cd8 t cell response to infection.costimulatory signals from dendritic cells (dcs) are required for naive t cells to respond to antigenic stimulation. to what extent dcs reactivate memory t cells during recall responses is not known. here, an in vivo depletion system has been used to analyze the role of dcs in reactivating cd8 memory t cells during recall responses to three different microbial infections. we show a profound decrease in the numbers of responding memory cd8 t cells in both lymphoid and nonlymphoid tissues during t ...200515894274
enhanced toll-like receptor responses in the absence of signaling adaptor dap12.dap12 is a signaling adaptor containing an immunoreceptor tyrosine-based activation motif (itam) that pairs with receptors on myeloid cells and natural killer cells. we examine here the responses of mice lacking dap12 to stimulation through toll-like receptors (tlrs). unexpectedly, dap12-deficient macrophages produced higher concentrations of inflammatory cytokines in response to a variety of pathogenic stimuli. additionally, macrophages deficient in spleen tyrosine kinase (syk), which signals d ...200515895090
comprehensive survey of pasteurized fluid milk produced in the united states reveals a low prevalence of listeria monocytogenes.a comprehensive survey was undertaken to generate contemporary data on the prevalence of listeria monocytogenes in pasteurized fluid milk produced in the united states. samples (5,519) near the sell-by expiration date were purchased at retail outlets over a 5-week period and analyzed for presence of l. monocytogenes. products consisted of whole milk, nonfat milk, and chocolate milk packaged in gallon, half gallon, quart, pint, and half-pint containers. samples were collected from both large and ...200515895729
distribution of listeria monocytogenes subtypes within a poultry further processing plant.samples from environmental sites and raw product in a chicken further processing plant were collected every 6 weeks for 12 months. each sample site was examined before and after a complete production shift. all samples were examined for the presence of listeria monocytogenes, which was detected in floor drains on the raw product side of the plant preoperation and in drains on both raw and cooked sides following 8 h of processing operation. l. monocytogenes also was detected in raw product and on ...200515895730
efficacy of electrolyzed water in inactivating salmonella enteritidis and listeria monocytogenes on shell eggs.the efficacy of acidic electrolyzed (eo) water produced at three levels of total available chlorine (16, 41, and 77 mg/ liter) and chlorinated water with 45 and 200 mg/liter of residual chlorine was investigated for inactivating salmonella enteritidis and listeria monocytogenes on shell eggs. an increasing reduction in listeria population was observed with increasing chlorine concentration from 16 to 77 mg/liter and treatment time from 1 to 5 min, resulting in a maximal reduction of 3.70 log cfu ...200515895731
postprocessing antimicrobial treatments to control listeria monocytogenes in commercial vacuum-packaged bologna and ham stored at 10 degrees c.the antilisterial effect of chemical dipping solutions on commercial bologna and ham slices, inoculated (3 to 4 log cfu/ cm2) after processing, was evaluated during storage in vacuum packages at 10 degrees c. samples were inoculated with a 10-strain composite of listeria monocytogenes and subsequently immersed (25+/-2 degrees c) for 2 min in 2.5% acetic acid (aa), 2.5% lactic acid (la), 5% potassium benzoate (pb), or 0.5% nisaplin (commercial form of nisin, equivalent to 5,000 iu/ml of nisin) so ...200515895732
prewashing with acidified sodium chlorite reduces pathogenic bacteria in lightly fermented chinese cabbage.efficacy of prewashing with acidified sodium chlorite (asc) for the sanitation of lightly fermented chinese cabbage was evaluated. the population of the natural microflora on the cabbage leaves was reduced about 2.0 log cfu/g just after washing with asc, a significant reduction compared with the control distilled water wash (p < or = 0.05). in the control experiment, viable aerobic bacteria increased gradually when incubated at 10 degrees c; however, asc-washed cabbage maintained a lower microbi ...200515895733
antimicrobial activity of nisin adsorbed to surfaces commonly used in the food industry.the adsorption isotherms of nisin to three food contact surfaces, stainless steel, polyethyleneterephthalate (pet), and rubber at 8, 25, 40, and 60 degrees c, were calculated. for all surfaces, the increase in temperature led to a decrease in the affinity between nisin and the surface. the rubber adsorbed a higher amount of nisin (0.697 microg/cm2) in comparison with pet (0.665 microg/cm2) and stainless steel (0.396 microg/cm2). adsorption of nisin to the stainless steel surface described l-2 ty ...200515895735
bacteriophage control of foodborne bacteriat.bacteriophages are measurable components of the natural microflora in the food production continuum from the farm to the retail outlet. phages are remarkably stable in these environments and are readily recovered from soil, sewage, water, farm and processing plant effluents, feces, and retail foods. purified high-titer phage lysates have been used for the species-specific control of bacteria during the pre- and postharvest phases of food production and storage. for example, the inhibition of the ...200515895751
unity in organisation and regulation of catabolic operons in lactobacillus plantarum, lactococcus lactis and listeria monocytogenes.global regulatory circuits together with more specific local regulators play a notable role when cells are adapting to environmental changes. lactococcus lactis is a lactic acid bacterium abundant in nature fermenting most mono- and disaccharides. comparative genomics analysis of the operons encoding the proteins and enzymes crucial for catabolism of lactose, maltose and threhalose revealed an obvious unity in operon organisation . the local regulator of each operon was located in a divergent tr ...200515900965
a multidomain fusion protein in listeria monocytogenes catalyzes the two primary activities for glutathione biosynthesis.glutathione is the predominant low-molecular-weight peptide thiol present in living organisms and plays a key role in protecting cells against oxygen toxicity. until now, glutathione synthesis was thought to occur solely through the consecutive action of two physically separate enzymes, gamma-glutamylcysteine ligase and glutathione synthetase. in this report we demonstrate that listeria monocytogenes contains a novel multidomain protein (termed gshf) that carries out complete synthesis of glutat ...200515901709
activated antigen-presenting cells select and present chemically modified peptides recognized by unique cd4 t cells.cd4 t cells recognized posttranslationally modified peptides of the protein hen egg-white lysozyme (hel), consisting of nitration of tyrosines and modifications of tryptophans in the t cell contact residues of the peptides. t cells were directed against modifications of a chemically dominant hel peptide as well as a minor hel peptide, bound to the class ii histocompatibility molecule i-a(k). the modified peptides were generated in vivo after immunization with native hel molecules or were generat ...200515901898
dynamic regulation of ifn-gamma signaling in antigen-specific cd8+ t cells responding to infection.ifn-gamma plays a critical role in the cd8(+) t cell response to infection, but when and if this cytokine directly signals cd8(+) t cells during an immune response is unknown. we show that naive ag-specific cd8(+) t cells receive ifn-gamma signals within 12 h after in vivo infection with listeria monocytogenes and then become unresponsive to ifn-gamma throughout the ensuing ag-driven expansion phase. ag-specific cd8(+) t cells regain partial ifn-gamma responsiveness throughout the contraction ph ...200515905520
the role of protein kinase a anchoring via the rii alpha regulatory subunit in the murine immune system.intracellular camp may inhibit t cell activation and proliferation via activation of the camp-dependent protein kinase, pka. pka signaling is maintained through interactions of the regulatory subunit with a-kinase anchoring proteins (akaps). we demonstrated that t cells contain akaps and now ask whether pka anchoring to akaps via the riialpha regulatory subunit is necessary for camp-mediated inhibition of t cell activation. we studied the immune systems of mice lacking the riialpha regulatory su ...200515905526
the antiapoptotic protein bcl-xl is dispensable for the development of effector and memory t lymphocytes.the antiapoptotic protein bcl-x(l) is induced in activated t lymphocytes upon costimulation through cd28, 4-1bb, and ox40. bcl-x(l) is also highly enriched in memory t lymphocytes. based on this body of evidence, it was thought that bcl-x(l) plays an essential role in the generation of effector and memory t lymphocytes. we report that mice with a conditional deletion of bcl-x in t lymphocytes develop a normal cd8(+) t cell response to listeria monocytogenes infection. furthermore, bcl-x conditio ...200515905539
risk factors for intramammary infections and relationship with somatic-cell counts in italian dairy goats.routine examination of milk was performed on five herds of lactating goats in northern italy as part of a milk quality-monitoring program in the year 2000. as part of the study, aseptic samples of foremilk were collected monthly from both half udders during the entire lactation for 305 goats, resulting in a total of 4571 samples. the samples were tested with cytological and bacteriological analyses to evaluate the relationship between mammary infections and somatic-cell count (scc; fossomatic (t ...200515907567
cationic microparticles consisting of poly(lactide-co-glycolide) and polyethylenimine as carriers systems for parental dna vaccination.cationic microparticles for dna adsorption were formulated by blending poly(lactide-co-glycolide) (plga) (50:50), with different cationic agents, either pei 25 kda (polyethylenimine) or ctab (cetyl-trimethyl-ammonium-bromide). the aim was to create adjuvant delivery systems increasing the efficiency of dna vaccines. microparticles formulated with 10% pei exhibited a highly positive zeta-potential, small particle sizes, in contrast to particles prepared with ctab, which revealed highly aggregated ...200515907586
contribution of nitric oxide to cpg-mediated protection against listeria monocytogenes.immunostimulatory cpg oligodeoxynucleotides (odn) improve host resistance to listeriae. cpg odn trigger immune cells to produce gamma interferon and "prime" host cells to secrete nitric oxide in response to bacterial exposure. cpg treatment does not protect inducible nitric oxide synthase 2 knockout mice, indicating that no is critical to cpg-mediated protection against listeriae.200515908417
analysis of the prevalence of tetracycline resistance genes in clinical isolates of enterococcus faecalis and enterococcus faecium in a japanese hospital.prevalence of seven tetracycline resistance (tc(r)) genes--tet(l), tet(m), tet(k), tet(o), tet(s), tet(t), and tet(u)--which are known to be distributed to gram-positive cocci was analyzed for 224 enterococcus faecalis and 46 enterococcus faecium clinical isolates obtained in a japanese hospital. any of the tc(r) genes was detected in 75.9% of all the enterococcal strains. the tet(m) was detected at highest rates in both e. faecalis (75.0%) and e. faecium (69.6%), followed by tet(l), which was h ...200515910229
characterization of anti-self cd8 t-cell responses stimulated by recombinant listeria monocytogenes expressing the melanoma antigen trp-2.a potential approach to activate tumor-specific t cells is to use live bacterial vectors to deliver appropriate antigens in a highly immunostimulatory context. we constructed a recombinant strain of listeria monocytogenes (rlm) expressing murine tyrosinase-related protein-2 (trp-2), a nonmutated melanocyte-derived differentiation antigen highly expressed in melanomas. immunization of c57bl/6 mice with this rlm strain efficiently primed cd8 t cells to recognize the mhc class i-restricted trp-2180 ...200515913853
uncaria tomentosa extract increases the number of myeloid progenitor cells in the bone marrow of mice infected with listeria monocytogenes.in this study, we demonstrated that uncaria tomentosa extract (ute) protects mice from a lethal dose of listeria monocytogenes when administered prophylactically at 50, 100, 150 and 200 mg/kg for 7 days, with survival rates up to 35%. these doses also prevented the myelosuppression and the splenomegaly caused by a sublethal infection with l. monocytogenes, due to increased numbers of granulocyte-macrophage progenitors (cfu-gm) in the bone marrow. non-infected mice treated with 100 mg/kg ute also ...200515914328
gastrointestinal phase of listeria monocytogenes infection. 200515916648
proteomic and microscopic analysis of biofilms formed by listeria monocytogenes 568.biofilm formation may be important in the colonization of the food-processing environment by the food-borne pathogen listeria monocytogenes. listeria monocytogenes 568 formed adherent multicellular layers on a variety of test surfaces following growth at 37 degrees c with multiple transfers of the test surface into fresh medium. microscopic examination of these adherent layers suggest that the cells were surrounded by extracellular material. the presence of a carbohydrate containing extracellula ...200515920617
[a new complex approaches for the identification of listeria isolated during production of fermented sausages].distribution l. monocytogenes and other listeria spp. in raw meat and during manufacturing of fermented meat products is investigated. the high contamination of raw materials and semi finished foods--in 36.5% of samples, ready-to-eat sausages--31.8% by listeria spp. is established. detection l. monocytogenes in 9.7% cases from the surfaces of equipment indicates the intensive circulation of listeriosis agents on meat plants. for identification 49 isolated strains the approach providing applicati ...200515921204
thalidomide enhances both primary and secondary host resistances to listeria monocytogenes infection by a neutrophil-related mechanism in female b6c3f1 mice.previously, we have reported that thalidomide can modulate the immune responses in female b6c3f1 mice. furthermore, thalidomide immunomodulation increased primary host resistance to intravenously infected listeria monocytogenes. the present study was intended to evaluate the mechanisms underlying the enhanced host resistance to l. monocytogenes by focusing on the neutrophils. female b6c3f1 mice were treated intraperitoneally with thalidomide (100 mg/kg) for 15 days. exposure to thalidomide incre ...200515921716
analysis of the murein of a listeria monocytogenes egd mutant lacking functional penicillin binding protein 5 (pbp5).cells of a mutant of listeria monocytogenes lacking functional pbp5, an enzyme with dd-carboxypeptidase activity, make thicker cells walls. in this study we show that the muropeptide profile of the mutant, obtained after hplc analysis of a muramidase digest of cell wall murein, differs from that for the wild type strain. the main differences embrace strongly reduced disaccharide-tripeptide content, strongly increased amounts of pentapeptide-containing muropeptides and a shift in profile from les ...200516599308
listeria monocytogenes: silage, sandwiches and science.listeria monocytogenes is amongst the most intriguing and well studied of the pathogenic bacteria. however, the understanding and perspective one has of l. monocytogenes depends to a large extent on the microbiological issues with which one is faced as a part of your professional duties. the focus of the veterinary clinician or investigator is likely to be foremost on the neurologic (circling disease) and reproductive diseases l. monocytogenes causes. to the food microbiologist, the principal co ...200516583783
[endocarditis due to listeria monocytogenes associated with aortic stenosis]. 200516419604
listeria monocytogenes as a probe of immune function.for almost half a century, the mouse model of listeria monocytogenes infection has been used to analyse both innate and adaptive components of immunity and to discover key immune genes. vast accumulated knowledge about the disease in mice provides a unique framework for identifying and characterising immune molecules using a variety of experimental approaches. to illustrate the range of questions that can be addressed using modern genetics and genomics tools, the authors provide an overview of t ...200516420751
listeria monocytogenes protein p60 affects hemolytic activity and uptake of bacteria by macrophages.bacillus subtilis strains expressing listeriolysin o (llo) and simultaneously llo and p60 protein were constructed. the effect of p60 protein on hemolytic activity and on the invasion of professional phagocytes was demonstrated in the absence of other virulence factors of l. monocytogenes. the hemolytic activity of llo in the presence of p60 protein decreased which indicates that p60 promoted adhesion and subsequent invasion of professional phagocytes.200516681150
susceptibility to mycobacterial infections due to interferon-gamma and interleukin-12 pathway defects.a case of interferon-gamma (ifn-gamma)/interleukin-12 (il-12) pathway defect is presented. pathophysiology, clinical characteristics, diagnostic test, and case management are reviewed. clinical pearls and pitfalls include: (1) a high probability of a defect in the ifn-gamma/il-12 cascade exists in patients with disseminated or recurrent infection due to poorly pathogenic mycobacteria or systemic infections caused by non-typhi salmonella species that are persistent and recurrent despite antibioti ...200516450579
respiratory infection of turkeys with listeria monocytogenes scott a.the pathogenesis of l. monocytogenes strain scott a was studied by challenging day-old male turkey poults by air sac inoculation with tryptose phosphate broth containing 10(0) cfu (control), 10(4), 10(5), and 10(6) cfu (low challenge), or 10(7) and 10(8) cfu (high challenge) of the scott a (serotype 4b) strain of l. monocytogenes. mortality at 2 wk postinfection (pi) ranged from 25% for low challenge to 100% for high challenge (p= 0.0001). gross and histopathological lesions were observed in hea ...200516404998
fosfomycin resistance proteins: a nexus of glutathione transferases and epoxide hydrolases in a metalloenzyme superfamily.three similar but mechanistically distinct fosfomycin resistance proteins that catalyze the opening of the oxirane ring of the antibiotic are known. fosa is a mn(ii) and k(+)-dependent glutathione transferase. fosb is a mg(2+)-dependent l-cysteine thiol transferase. fosx is a mn(ii)-dependent fosfomycin-specific epoxide hydrolase. the expression, purification, kinetic, and physical characteristics of six fosfomycin resistance proteins including the fosa proteins from transposon tn2921 and pseudo ...200516399398
[neurolisteriosis in adults: report of six clinical cases].listeria monocytogenes shows a special attraction to infect the central nervous system and its meningeals coats. it affects newborn as well as elderly people, patients with deficiencies in their cellular immune systems, and healthy adults. it presents most commonly as an acute meningitis, although it can present itself as cerebritis, brain stem encephalitis (rhomboencephalitis), and exceptionally as myelitis.200516400430
antimicrobial spectrum and potency of dalbavancin tested against clinical isolates from europe and north america (2003): initial results from an international surveillance protocol.dalbavancin is a bactericidal dimethylaminopropyl amide glycopeptide derivative possessing an extended serum elimination half-life in humans that allows once-weekly dosing for the therapy of gram-positive infections. strains from this baseline surveillance protocol in north america (na; usa and canada) and europe (eu, 14 countries) were sampled in 2003. a total of 7,765 gram-positive isolates (3,695 from na and 4,070 from eu) were tested by reference broth microdilution methods against dalbavanc ...200516433188
detection and identification of intestinal pathogenic bacteria by hybridization to oligonucleotide microarrays.to detect the common intestinal pathogenic bacteria quickly and accurately.200516437687
wasp family proteins act between cytoskeleton and cellular signaling pathways.this review considers the proteins of the wasp (wiskott-aldrich syndrome protein) family and their role in the regulation of actin-based motility. it contains detailed classification of the wasp family proteins and data on their subcellular localization. impairments of expression of the wasp family proteins cause certain cell pathologies. the review also deals with domain organization of these proteins and proteins interacting with various domains of the wasp proteins. special attention is given ...200516417451
how seryl-phosphorylated hpr inhibits prfa, a transcription activator of listeria monocytogenes virulence genes.listeria monocytogenes prfa, a transcription activator for several virulence genes, including the hemolysin-encoding hly, is inhibited by rapidly metabolizable carbon sources (glucose, fructose, etc.). this inhibition is not mediated via the major carbon catabolite repression mechanism of gram-positive bacteria, since inactivation of the catabolite control protein a (ccpa) did not prevent the repression of virulence genes by the above sugars. in order to test whether the catabolite co-repressor ...200516415595
trimethoprim-sulfamethoxazole-induced aseptic meningitis.we present a 46-year-old african-american man with aids who was admitted on two different occasions within three weeks for signs and symptoms of meningitis after using trimethoprim/sulfamethoxazole (tmp/smx). tmp/smx is primarily used for the treatment of pneumocystis carinii pneumonia prophylaxis in aids patients. drug-induced aseptic meningitis (diam) is commonly seen with nonsteroidal anti-inflammatory drugs (nsaids), antibiotics (with tmp/smx being the most frequently implicated), intravenou ...200516396068
evaluation of farm management practices as risk factors for clinical listeriosis and fecal shedding of listeria monocytogenes in ruminants.to assess seasonal variation in prevalence of listeria monocytogenes on ruminant farms and identify management practices associated with ruminant listeriosis and fecal shedding of l. monocytogenes.200516350271
[meningoencephalitis by listeria in the lupus disease].we present a patient with lupus nephropathy of 20 years of evolution in treatment with oral steroids who developed a meningoencephalitis associated to bacteraemia by listeria monocytogenes. the patient was treated successfully with gentamicin and ampicillin for 6 weeks. infection by listeria monocytogenes occurs more frequently in individuals with some form of immunodeficiency like lupus disease, with a mortality around 30%.200516351491
listeria monocytogenes as the possible cause of the spontanous abortion in female of the fertile age.listeria monocytogenes is a gram-positive, weakly pathogenic bacterium able to grow also at the temperature of 40 degrees c. a man most often gets affected by consuming contaminated food and water. animals can carry bacteria although they have not to appear ill, then their meat and milk products are the source of infection of human being. the disease most often attacks with the weak immune system, newborns and pregnant women. that what is significant, listeria penetrates through the placenta and ...200516351605
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