Publications

midbrain lesions, dopamine and male sexual behavior.	destruction of the medial preoptic area (mpoa) eliminates mating in male rats and this region is believed to play a major role in the control of male sexual behavior. efferents from the mpoa pass through and/or terminate in 4 midbrain regions: the dorsolateral tegmentum (dlt), the central gray, and the a9 and a10 areas. the present study reports the effects of bilateral destruction of each of these midbrain regions on brain catecholamines and sexual behavior in male rats. dlt lesions eliminated ...	1986	3524604
a genetic determinant required for continuous reinfection of adjacent cells on large plasmid in s. flexneri 2a.	we have identified a region (virg) on the 230 kb virulence plasmid of s. flexneri that is required for cell-to-cell spread of the bacterium. tn5 insertions into this region result in avirulent mutants that can initially invade and multiply in epithelial cells, but tend to lose active movement and tend to localize within the cytoplasm, where they are gradually extinguished without infecting adjacent cells. the virg region was localized to within 4 kb and may contain a single cistron. sequences hy ...	1986	3524856
crp genes of shigella flexneri, salmonella typhimurium, and escherichia coli.	the complete nucleotide sequences of the salmonella typhimurium lt2 and shigella flexneri 2b crp genes were determined and compared with those of the escherichia coli k-12 crp gene. the shigella flexneri gene was almost like the e. coli crp gene, with only four silent base pair changes. the s. typhimurium and e. coli crp genes presented a higher degree of divergence in their nucleotide sequence with 77 changes, but the corresponding amino acid sequences presented only one amino acid difference. ...	1986	3525518
hemodynamics of the legs and clinical symptoms following regional blocks for transurethral surgery.	in a prospective clinical study we compared the hemodynamics and clinical symptoms following regional blocks and general anesthesia. 115 patients undergoing transurethral resection of the prostate were randomized to spinal (n = 62) and epidural (n = 53) blocks. an additional 10 patients received general anesthesia. calf arterial flow, determined by strain gauge plethysmography (sgp), was similar pre- and postoperatively in the regional block groups but decreased in the general anesthesia group ( ...	1986	3527712
[effect of dichroa febrifuga l. on chloroquinsensible and chloroquinresistant malaria parasites].		1986	3528514
comparative histochemical distribution of nerve fibres storing noradrenaline and neuropeptide y (npy) in human ovary, fallopian tube, and uterus.	nerves containing noradrenaline were studied by formaldehyde-induced fluorescence and neuropeptide y (npy) was visualised by immunohistochemistry in the human ovary, fallopian tube and uterus. all structures were richly supplied with noradrenergic fibres closely associated with the vascular and non-vascular smooth musculature. npy-containing nerve terminals were consistently fewer, particularly in the ovary. the best developed nerve supply was found in the tubal isthmus and uterine cervix. vesse ...	1986	3528701
age-related changes in tissue metabolic rates and sensitivity to insulin in the chicken.	in vitro metabolic studies were conducted with tissues from broiler pullets at 4, 8, and 12 weeks of age. in order to accurately assess plasma insulin at each of these ages, blood samples were withdrawn via indwelling venous catheters at 30-min intervals for 6 hr, and plasma was assayed by a homologous chicken insulin (cins) radioimmunoassay. birds (n = 6) were sacrificed 24 hr following blood sampling and livers, hearts, and abdominal fat pads removed for metabolic studies. tissue slices of rem ...	1986	3529070
[effect of immunization with an enterobacterial vaccine on the rate of plating out of shigella from the intestinal mucosa of mice orally infected].	the oral and subcutaneous immunization of mice with the vaccine prepared from s. minnesota strain r595, chemotype re, known as enterobacterial vaccine, was found to significantly decrease the number of shigellae in cultures obtained by the inoculation of homogenized mucosal samples taken from the large intestine of mice, the vaccine prepared from s. minnesota isogeneous strain sf 1111 with the intact structure of lipopolysaccharide had no such activity. antisera, obtained by the immunization of ...	1986	3529760
[intrahepatic cholestasis due to shigella in an infant].		1986	3530915
comparison of dna probes and the sereny test for identification of invasive shigella and escherichia coli strains.	forty-two shigella and 29 escherichia coli strains were screened for invasiveness in the sereny test and for hybridization with two recently described dna probes for the invasiveness plasmid. both probes produced identical results. all sereny-positive strains hybridized with both dna probes. three sereny-negative strains also hybridized with the probes, suggesting that there are strains containing the invasiveness plasmid that are not pathogenic in animal models.	1986	3531233
characterization of shigella flexneri-specific murine monoclonal antibodies by chemically defined glycoconjugates.	chemically defined glycoconjugates are demonstrated to have considerable potential for selecting hybridoma antibodies directed toward o-antigenic determinants, especially when used in combination with a panel of well-characterized lps molecules. monoclonal antibodies specific for the shigella flexneri o-antigens of serogroup 5b, variants x and y, were generated after immunization of balb/c mice with killed bacterial cells, and active hybrids were selected on the basis of elisa performed with the ...	1986	3531329
[phagocytizing blood cells in dysentery].		1986	3532361
[detection of immunosuppression factors in shigella sonnei].	a factor, making noninvasive shigellae and other bacteria capable of suppressing immunological memory and secondary immune response manifested as delayed hypersensitivity, has been detected in the germ-free filtrates of the broth cultures of invasive s. sonnei.	1986	3532643
[general and local reactions in the proteolytic system during the development of experimental pneumonia].	proteolytic enzymes and their inhibitors were studied in the serum and bronchoalveolar lavage of rats with acute and chronic experimental pulmonary inflammation. an increase in the inhibitory potential of the blood serum and bronchoalveolar lavage at the acute stage of inflammation was shown. at the chronic stage of pulmonary inflammation the level of the main inhibitors decreased to normal or zero values in the blood and lavage fluid, respectively. it was observed that alpha 1-proteinase inhibi ...	1986	3533173
epidemic reiter's syndrome following an outbreak of shigellosis.	we prospectively studied the occurrence of reiter's syndrome (rs) or reactive arthritis (rea) in 205 of 349 cruise-ship passengers who attended a buffet ashore and developed shigella flexneri 2a enteritis. five passengers probably had rs/rea and 16 were possible or doubtful cases of rs/rea. hla-b27 was identified in 4 of 5 probable rs cases, but was not present in any of the 16 possible or doubtful cases nor in any of 20 passengers (controls) without any symptoms of rs/rea. there was no statisti ...	1986	3533609
lack of serologically defined arthritogenic shigella flexneri cell envelope antigens in post-dysenteric arthritis.	post-dysenteric or reactive arthritis (rea) is closely associated with hla-b27. this histocompatibility antigen is heterogeneous and consists of 2 serologically defined variants: b27m1+m2+ and b27m1+m2-. this paper gives a qualitative evaluation of the antibodies present in the sera of 62 patients with dysentery due to shigella flexneri 2a, a known arthritogenic bacterium. the patients were classified in 4 groups: b27m1+m2+rea+ (n = 5), b27m1+m2+rea- (n = 7); b27m1+m2-rea- (n = 1); b27-rea- (n = ...	1986	3533766
enzyme immunoassay for detection of salmonella in foods: collaborative study.	a collaborative study was performed in 25 laboratories to validate an enzyme immunoassay (eia) procedure utilizing 2 specific monoclonal antibodies for rapid detection of salmonella in foods. the eia was compared with the standard culture procedure for detection of salmonella in 6 food types: ground black pepper, soy isolate, dried whole eggs, milk chocolate, nonfat dry milk, and raw deboned turkey. uninoculated and inoculated samples were included in each food group analyzed, with the exception ...	1986	3533896
[evaluation of the antibiotic sensitivity of pseudomonas sp. using the api-atb-pse system. comparison with diffusion and the reference agar dilution method].	in vitro activity of twelve antibiotics (ticarcillin, mezlocillin, azlocillin, piperacillin, cefoperazone, cefsulodin, ceftazidime, gentamicin, netilmicin, pefloxacin and ciprofloxacin) was determined by measuring minimum inhibitory concentrations (mics) using agar dilution according to who recommendations, agar diffusion and the api-atb-pse system. one-hundred and forty-two pseudomonas strains were studied. five species of pseudomonas were represented, i.e. aeruginosa, maltophilia, cepacia, stu ...	1986	3534715
serogroup frequency and drug sensitivity of shigella strains encountered at the university hospital of the west indies.		1986	3535257
shigella dysenteriae type 1 enterocolitis.	shigella dysenteriae type 1 is much more virulent than shigella flexneri and sonnei which are endemic in australia. this report describes a 22 year old woman who acquired shigella dysenteriae type 1 whilst travelling in india. during the course of her illness, she developed severe enterocolitis for which a subtotal colectomy was performed. the illness resembled fulminant ulcerative colitis and its infectious nature was difficult to establish because several fecal cultures failed to grow the path ...	1986	3535768
interleukin-1 (22-k factor) induces release of granulocyte-macrophage colony-stimulating activity from human mononuclear phagocytes.	an electrophoretically pure preparation of natural human interleukin-1 (il-1) was shown to stimulate in vitro colony formation in human bone marrow cultures. day 4 myeloid cluster-forming cells (cfc), as well as early (day 7) and late (day 10) granulocyte-macrophage colony-forming units (cfu-gm) were stimulated in a dose-dependent fashion. at optimal concentrations of il-1, the number of day 4 cfc reached 72%, the number of day 7 cfu-gm reached 32%, and the number of day 10 cfu-gm reached 80% of ...	1986	3535928
studies on the mechanics of the normal human middle ear.	the middle ear was studied in temporal bone preparations using a laser-doppler interferometer. for measurements at a sound level of 80 db spl this method proved to be very reliable, as was shown by good reproducibility of results in experiments over more than 6 hours. the vibrations of the tympanic membrane and stapes footplate were studied from 200 hz to 10 khz and the results demonstrate a piston-like movement of the stapes footplate up to 120 db spl. the damping effect of the normal ear is lo ...	1986	3536194
widal-type serology using live antigen for diagnosis of shigella flexneri dysentery.		1986	3536499
salmonella/microsome mutagenicity tests of heat-processed milk samples.	samples of whole milk were heat treated by commercial heat-sterilization, by commercial heat-pasteurization or by a laboratory heat-pasteurization (65 degrees c for 30 min). each sample was tested for mutagenicity in salmonella typhimurium strains ta98 and ta100 with and without s-9 mix. dichloromethane extracts of milk heated at 100, 135 and 150 degrees c for 5 hr were also tested for mutagenicity using the same assay. none of these samples exhibited mutagenicity in the ames assay used.	1986	3536696
secretory immunoglobulin a response following peroral priming and challenge with shigella flexneri lacking the 140-megadalton virulence plasmid.	this study evaluates the ability of noninvasive shigella spp., lacking the 140-megadalton virulence plasmid, to elicit a mucosal immunoglobulin a immune response in the intestine. for these studies, we used shigella flexneri m4243a1 (which lacks the plasmid and is sereny test negative) to prime and challenge three groups of rabbits perorally. both primary and immunoglobulin a memory responses were detectable in these secretions. these findings indicate that a mucosal memory response can be prime ...	1986	3536753
a solid-phase enzyme immunoassay for the determination of igm and igg antibodies against translation products of pre-s1 and pre-s2 regions of hepatitis b virus.	the envelope of hepatitis b virus is coded for by pre-s1, pre-s2 regions and the s gene. a method was developed to determine antibody to the product of pre-s1 region (anti-pre-s1) and antibody to the product of pre-s2 region (anti-pre-s2), either of igm or igg class, by a solid-phase enzyme immunoassay. for the determination of anti-pre-s1, tubular particles containing translation products of pre-s1, pre-s2 regions and the s gene were broken into constituent envelope polypeptides and immobilized ...	1986	3537138
a solid-phase enzyme immunoassay for the determination of igm and igg antibodies against translation products of pre-s1 and pre-s2 regions of hepatitis b virus.	the envelope of hepatitis b virus is coded for by pre-s1, pre-s2 regions and the s gene. a method was developed to determine antibody to the product of pre-s1 region (anti-pre-s1) and antibody to the product of pre-s2 region (anti-pre-s2), either of igm or igg class, by a solid-phase enzyme immunoassay. for the determination of anti-pre-s1, tubular particles containing translation products of pre-s1, pre-s2 regions and the s gene were broken into constituent envelope polypeptides and immobilized ...	1986	3537138
production of shiga toxin and other cytotoxins by serogroups of shigella.	we measured the cytotoxic activity of 119 strains of shigella by using a quantitative [3h]thymidine-labeled hela cell assay. we assayed 13 strains of shigella dysenteriae 1; 18 strains of s. dysenteriae types 2 and 3; and 88 strains of shigella sonnei, shigella flexneri, and shigella boydii. strains of s. dysenteriae 1 demonstrated high levels of cytotoxicity (geometric mean, 10(5.04) cd50/mg of protein; range, 10(3.95)-10(6.10). cytotoxic activities of the non-type 1 strains of s. dysenteriae a ...	1986	3537154
surface hydrophobicity of plasmid-carrying virulent shigella flexneri and their avirulent variants.	five pairs of strains of s. flexneri each differing in the colour of their colonies after growth on congo red agar have been tested for their ability to cause keratoconjunctivitis in the guinea pig eye, for the presence of the 140 md virulence plasmid, for the presence of the virulence marker antigen, and for their ability to adsorb to hydrophobic surfaces (cellulose nitrate filters and phenyl sepharose). the results suggest that the presence of the 140 md virulence plasmid provides the bacteria ...	1986	3537274
modelling of dna sedimentation profiles from irradiated eukaryotic cells.	a simple mathematical model of dsb distributions in dna from irradiated interphase eukaryotic cells is considered and an approximate description of resulting dna sedimentation profiles is derived. the model takes into account the differences found between the radiosensitivities of heterochromatin and euchromatin and is based, contrary to the usual approach, on a non-homogeneous distribution of dsb in eukaryotic dna. comparison with some experimental dna sedimentation profiles prefers the propose ...	1986	3537531
phenotypic characterization of the thymus microenvironment study of the human thymus architecture.	phenotypic characterization of the human thymic microenvironment shows the heterogeneity of the epithelial component. using monoclonal antibody l191, we define here a new antigen common to the flat epithelial cells lining up the thymic capsule and septum, stellate epithelial cells from the subcapsular area and the medulla (m), but lacking on stellate epithelial cells from the inner cortex. thus, this cell population can be characterized by a series of antigens (te-4 and p19--shared with htlv--pr ...	1986	3541301
[ultrastructural study of intestinal bacteria grown on endo- and levine-type nutrient media based on nonfood raw materials].	the comparative electron-microscopic study of several test strains (salmonella typhi h-901, shigella flexneri la 8516, and escherichia coli 055) grown in experimental endo and levine media prepared on the basis of raw materials unsuitable for human consumption and in commonly used similar media prepared on the basis of sprat hydrolysate has shown the test strains grown in media containing aminopeptide and fodder yeast hydrolysate to retain their typical ultrastructure, which confirms the possibi ...	1986	3541464
[characteristics of erythrocyte diagnostic agents studied using optical methods].	the quantitative characterization of erythrocyte diagnosticums (ed) has been made by optical methods (light microscopy with the use of an image analyzer, model magiscan 2, and the opacity spectrum technique). the following parameters of ed have been determined: the average of the major axis (5.25 +/- 0.57 micron for ed from shigella sonnei and 5.53 +/- 0.50 micron for ed from shigella flexneri), the ratio of semiaxes (p approximately equal to 3), the major axis length distribution, the refractiv ...	1986	3541465
[chromosomal changes in patients with acute dysentery and their relation to immunologic shifts].	the correlation between the dynamics of the occurrence of the class of lymphocytes without chromosomal associations and with two associating acrocentric chromosomes (cl0 + 2) and the course of the disease, as well as the development of immunity in patients with acute dysentery, has been detected. the positive correlation between the occurrence of cl0 + 2 and cell-mediated immunity reactions presupposes the possibility of using this cytogenetic characteristic for evaluating the cell-mediated immu ...	1986	3541467
[bacteriological study of shigellosis in the lake kivu area (central africa). developments in the last 15 years (1968-1983)].	1913 shigella strains were isolated from 1968 to 1983 in lake kivu area (central africa). distribution and evolution of serotypes, biotypes and antibiotypes were studied. the majority of serotypes and biotypes were stable. however, few of these strains showed variations, among them shigella dysenteriae serotype 1, causing epidemic dysenteriae since 1981. epidemiologic survey of shigellosis in kivu seems to be rather underdeveloped (4 bacteriology laboratories for an area of 10 million inhabitant ...	1986	3542256
effects of bacterial lipopolysaccharide on the binding of lymphocytes to endothelial cell monolayers.	preincubation of human umbilical vein endothelial cell (ec) monolayers with 1 ng to 10 micrograms/ml lipopolysaccharide (lps) increased the binding of t lymphocytes to ec. the effect was maximal at lps concentrations of 0.1 to 10 micrograms/ml, and occurred with lps derived from escherichia coli (serotypes 0111:b4 and 0127:b8), shigella flexneri (serotype 2a), serratia marcescens (serotype 0:3), and yersinia entercolitica (serotype 0:3). the increased binding appeared to be mediated primarily th ...	1986	3484495
improved survival after allogeneic small intestinal transplantation in the rat using cyclosporine immunosuppression.	we investigated the effect of cyclosporine on survival after intestinal transplantation between histoincompatible brown norway (agb3/3) and lewis (agb1/1) rats. intestinal grafts were primarily vascularized (by microsurgical techniques) and interposed isoperistaltically in the recipient's jejunum. all animals were weighed and observed daily. survival of recipients given cyclosporine 20 mg/kg/d (112 +/- 92 days, n = 10) was significantly longer (p less than .02) than that of recipients given no d ...	1986	3485711
characterisation of haemophilus spp. isolated from infant conjunctivitis.	haemophilus strains isolated from children under the age of 11 months with conjunctivitis were characterised by biotype, sugar fermentation, plasmid pattern and outer-membrane-protein profiles. h. influenzae was the most common species identified and was separated into 14 groups based on sugar fermentation and biotype patterns and into more than 20 groups when plasmid and outer-membrane-protein profiles were included. small (mol. wt less than 10 x 10(6)) plasmids were identified in 11 of 34 (32% ...	1986	3486294
possible role of protein phosphorylation in the mitogenic effect of high density lipoproteins on cultured vascular endothelial cells.	the implication of protein phosphorylation in the mitogenic action of high density lipoproteins (hdl) on bovine vascular endothelial cells was investigated by incubating endothelial cell cultures in the presence of 32p-labeled phosphoric acid. the incorporation of 32p into proteins was measured after fractionation by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate and autoradiography of the gel. in endothelial cells seeded at low density and made quiescent by serum s ...	1986	3486870
acrocentric/18p translocation in two mentally retarded males. delineation of the adult phenotype.	in this report we describe two adult male patients with a chromosomal rearrangement involving the short arm of chromosome 18 and an acrocentric chromosome. in addition to moderate mental retardation and verbal disability they presented dysmorphic stigmata similar to those found in the noonan syndrome.	1986	3490204
live dysentery vaccine "vadizen" (t32-istrati) immunogenicity against shigella-like challenge in animals.		1986	3300598
ultrastructural study on the bacterial symbiont in giardia lamblia.		1986	3300600
osteogenic sarcoma and soft tissue myxoma in a patient with fibrous dysplasia and hemoglobins jbaltimore and s.	a 41-year-old man with recognized polyostotic fibrous dysplasia since late childhood developed fibroblastic osteogenic sarcoma in the left tibia. four months after the initial diagnosis, an intramuscular myxoma was discovered in the left thigh. twenty years previously he had been found to be heterozygous for hemoglobins jbaltimore and s. malignant transformation in fibrous dysplasia is unusual and may be associated in some individuals with prior irradiation. soft tissue myxomas associated with f ...	1986	3456858
alpha-transforming growth factor secreted by untransformed bovine anterior pituitary cells in culture. ii. identification using a sequence-specific monoclonal antibody.	untransformed bovine anterior pituitary cells cultured in serum-free defined medium secrete an epidermal growth factor (egf)-like peptide with an amino acid composition similar to rat or human alpha-transforming growth factor (alpha tgf). to further characterize the bovine pituitary alpha tgf, it was compared to a human alpha tgf partially purified from the conditioned medium of a human melanoma cell line. an anti-alpha tgf monoclonal antibody, mf9, was produced from hybridomas derived from mice ...	1986	3464598
[possibility of overcoming of acnu resistance in an acnu-resistant subline of c6 rat glioma].	membrane-modifying agents such as reserpine, calcium antagonists (nicardipine, verapamil) and calmodulin inhibitor (trifluoperazine) were found to enhance the cytotoxicity of acnu in vitro and in vivo in acnu-resistant c 6 (c 6/acnu) glioma. in in vitro experiments, uptake and retention studies with [14c] acnu revealed that intracellular uptake and retention of acnu in c 6 cells were larger than those in c 6/acnu cells, and that these membrane-modifying agents increased the cellular uptake and r ...	1986	3468959
canine lymphoma: clinical considerations.		1986	3333887
canine lymphoma: clinical considerations.		1986	3333887
plasmid-mediated contact haemolytic activity in shigella species: correlation with penetration into hela cells.	the main feature of virulent strains of shigella is their ability to invade eucaryotic cells. this phenotype is both plasmid-mediated and temperature-regulated. in the present report, we demonstrate a plasmid-mediated and temperature-regulated haemolytic activity in s. flexneri, s. dysenteriae and s. sonnei. detection of this haemolytic activity requires centrifugation of suspensions containing bacteria and erythrocytes, followed by incubation of the pellets at 37 degrees c. no soluble intra- or ...	1986	3322171
invasion of epithelial cells by shigellae.		1986	3322174
plasmid-mediated intracellular multiplication of shigella flexneri.		1986	3322175
study of ileostomy in dogs, functional aspects, optic and scanning electronic microscopy.	containing ileostomy has been a permanent aim of researchers. different technics for containing in the ileal derivations has been proposed, very little filled the requisites, but no one reached the desired aim, and the problem continues unsolved. the authors, based in experimental study in two groups of dogs submitted to ileostomy, with and without preservation of ileal pylorus, observed the following parameters: weight dog clinical evaluation of animals (diarrhea, infection of surgical wound an ...	1986	3435265
resuscitation: a matter of life and death.		1986	260180
sarcoidosis: it strikes at many organs.		1986	260174
is this the face?		1986	260112
[benign vascular tumor of the stomach].		1986	176499
phase ii trial of 7-day continuous 5-fluorouracil infusion in the treatment of advanced colorectal carcinoma.	thirty-eight patients with advanced colorectal adenocarcinoma were entered on a phase ii trial of 5-fluorouracil (5-fu) in continuous infusion, using a portable pump. half of the patients had been pretreated (n = 19) and 16 of them had received intravenous bolus 5-fu alone or in combination. at the first cycle patients received continuous intravenous 5-fu at the dose of 650 mg/m2 per day for 7 consecutive days. doses were escalated during the following cycles and adjusted according to the toxici ...	1986	1542491
the aberdeen formula: revision of nursing workload per patient as a basis for staffing.		1986	260083
potassium pooling in non-rigid parenteral fluid containers.		1986	260935
evaluation of the effectiveness of ammonium bifluoride treatment in the cleaning of parenteral glassware by an automatic particle counting technique.		1986	260934
immunity: autoimmunity, isoimmunity, and immunodeficiency in the foal.		1986	260999
[immunologic studies in polymyositis syndromes].	sixteen cases of polymyositis were submitted to study. out of these sixteen, nine showed no clinical or paraclinical evidences of cancer, and seven presented an associated cancer. in the first group, a normal immune response was observed, whereas in the second group this response was frankly depressed. emphasis is laid on the significance of the immune response, not only in the diagnosis but as a fundamental factor in prognosis and treatment as well. an early treatment of polymyositis without ca ...	1986	262355
selecting staff for hospice work.		1986	260082
nursing care study: industrial burns.		1986	260075
[clinicopathological analysis of fatal cerebellar hematomas].	an analysis is made of the clinical observations and pathological findings in 16 fatal cerebellar hematomas admitted at the instituto de neurología in montevideo, over a period of 23 years. the absence of definitive lesions of the brainstem in the majority of cases, in spite of the rich symptomatology of brainstem disfunction (horizontal gaze palsies, vegetative syndrome decerebrate movements) is stressed, as well as the rapid evolution of those cases with intraventricular haemorrhage. missed cl ...	1986	262357
[neonatal heart rate variability].		1985	261135
[the kansai area midwives enthusiastically supporting breast feeding. encounter with the okeya technic for breast feeding and reinforced professional standing of midwifery. a discussion].		1985	261123
a serological assay for the detection of cell surface receptors of nerve growth factor.	when single-cell suspensions prepared from embryonic day 8 (e8) chick sensory ganglia are incubated with nerve growth factor (ngf), anti-ngf antiserum, and complement, an ngf-dependent cytotoxic kill of 20 (+/- 3)% of the ganglia cells is observed. this percentage is increased by a factor of two when only the neuronal cells are tested. no kill is observed on the nonneuronal cell population representing 50% of the ganglia dissociate. when e8 sensory ganglia cells are cultured in the presence of n ...	1985	219297
[radiofrequency thermocoagulation in occipital spondylotic neuralgia].		1985	262059
the anatomy of a smile.	the anatomy of the smile is an integral part of dentistry. its understanding involves close scrutiny of all elements of the oral region. it is not enough to establish the size of teeth based on the high and low lip lines, size of the mouth, and a shade to blend with the age and complexion. to create a harmonious smile the dentist must maintain or create the normal curvature of the lips, proper exposure of the red zone of the lips, an undistorted philtrum, and undisturbed nasolabial grooves. thes ...	1985	271728
aorta-coronary bypass grafting for takayasu's aortitis.		1985	1346542
properties and regulation of c-1-fructose-1,6-diphosphatase from spinach chloroplasts.	chloroplast fructose diphosphatase (ec 3.1.3.11) was purified according to the procedures of racker and schroeder [1] and buchanan et al. [2] and the properties compared. neither preparation contained fructose diphosphatase from the cytoplasm. the preparations had similar molecular weights, ph optima, affinites for fructose diphosphate and mg-2+ and were similarly activated by edta, dithiothreitol and cystamine. mg-2+, fructose diphosphate and dithiothreitol all activate chloroplast fructose dip ...	1985	238625
program-level evaluation using management information.	this paper has summarized the development of a drug abuse treatment program mis, and has shown that one can initially start with a manual system, use it to advantage, and then move into computer programming. in larger programs, it will most likely be necessary to use the computerized system. however, the author has found that implementation costs have not been great, and the system has certainly been worthwhile in light of the dimensions that have been added to the program. the computer mis that ...	1985	262074
[subcutaneous aspergillosis simulating erythema nodosum].		1985	261930
[what is spri? (sjukvårdens planerings- och rationaliseringsinstitut) (nursing care planning and rationalization institute)].		1985	261486
[effects of different postures on left ventricular function during various trimesters of pregnancy].		1985	261924
shigella flexneri type 2 infection in captive nonhuman primates.		1985	17422604
[prognostic indices of acute lymphoblastic leukemia in the child. proposal for a prognostic classification and case contribution].		1985	271763
[a new serotype of shigella flexneri in czechoslovakia?].		1985	3155665
synthesis of new polyoxin derivatives and their activity against chitin synthase from candida albicans.	two analogues of l-alanylpolyoxin c with a modified peptide bond were synthesized and tested for inhibition of chitin synthase in candida albicans. n-methylation of the peptide bond (compound 13) or the replacement of it by nh2ch2 (compound 9) led to loss of activity in the enzyme assay. a novel analogue (compound 5) of nikkomycin was synthesized from uracil polyoxin c and (2s,3r)-3-hydroxyhomotyrosine, a component of echinocandin c. despite high activity in the chitin synthase assay, 5 had no i ...	1985	3156247
distribution of sensory receptors in the flexor carpi radialis muscle of the cat.	the structures and distribution of encapsulated muscle receptors were examined in serial transverse sections of flexor carpi radialis in the adult cat. four types of receptors (muscle spindles, golgi tendon organs, paciniform, and pacinian corpuscles) were identified. their structures resembled those encountered in other limb muscles. pacinian corpuscles were rare and occurred only in the external fascial coat of the muscle near its origin. the other three receptor types were distributed in an u ...	1985	3156250
expression of laminin receptor in normal and carcinomatous human tissues as defined by a monoclonal antibody.	it has been hypothesized that epithelial and endothelial cells interact with the laminin component of basement membranes via a cell surface laminin receptor molecule. it has also been proposed that the expression of this molecule may be involved in the invasion of carcinoma cells from their tissue of origin and their subsequent penetration through blood vessel basement membranes. we report here the use of a monoclonal antibody, lr-3, to define the expression of laminin receptor in normal, dyspla ...	1985	3157447
characterization of protein kinase c and its role in catecholamine secretion from bovine adrenal-medullary cells.	protein kinase c activity towards exogenous histone was detected in a cytosolic fraction of bovine adrenal medulla. the enzyme was dependent on ca2+ and phosphatidylserine for its activity, with half-maximal activation being achieved at approx. 18 microm free ca2+ and 8 micrograms of phosphatidylserine/ml. both diolein and 4 beta-phorbol 12-myristate 13-acetate (tpa) decreased the ca2+ requirement of the enzyme, half-maximal activation being obtained at approx. 12 microm and 9 microm free ca2+ r ...	1985	3159383
synthesis of aerobactin and a 76,000-dalton iron-regulated outer membrane protein by escherichia coli k-12-shigella flexneri hybrids and by enteroinvasive strains of escherichia coli.	one of the chromosomal segments associated with the virulence of shigella flexneri and transferred to escherichia coli k-12 by conjugation has been shown to code for the production of aerobactin and for the synthesis of an iron-regulated 76,000-dalton (76k) outer membrane protein. analysis of various e. coli k-12-s. flexneri transconjugants showed that the genes involved with the synthesis of aerobactin and with the production of the 76k protein were linked to the mtl region of the s. flexneri c ...	1985	3159680
metabolism of thymoxamine. i. studies with 14c-thymoxamine in rats.	thymoxamine is rapidly and completely absorbed in rats. it is a prodrug which does not enter the systemic circulation in its unchanged form. after either oral or intravenous administration it undergoes rapid and intense metabolism involving four biotransformation reactions: enzymatic hydrolysis to the corresponding phenol (metabolite i), monodemethylation to metabolite ii, sulfate conjugation of i and ii (metabolites iii and iv) and conjugation of i and ii with glucuronic acid (metabolites v and ...	1985	3161738
bronchiolar and alveolar lesions in the pathogenesis of crocidolite-induced pulmonary fibrosis in mice.	asbestosis is generally considered to result in restrictive pulmonary disease associated with interstitial fibrosis. recently, however, attention has focused upon bronchiolar lesions and concomitant obstruction to air flow. the responses of conducting airways and alveoli were studied over a 20 week period following instillation of crocidolite to mice. the location of the lesions and the sequential inflammatory changes were studied by bronchoalveolar lavage, light and electron microscopy; regener ...	1985	3003309
[the kallikrein-kinin system in acute peritonitis and methods of correcting its disorders].	in experimental fecal peritonitis in 140 albino rats the kallikrein-kinin system was shown to undergo the following changes: its inhibition during the first day was followed by an activation on the second day and inanition on the third day. under conditions of hyperbaric oxygenation the normalizing effect on the kinin system was observed during two days. the application of salicylates against the background of hbo was shown to enhance the normalizing effect which lasted for three days.	1985	3004000
fluctuation of specific iga antibodies in human milk.	the concentration of secretory iga and the levels of iga specific antibodies against escherichia coli labile-toxin, shigella flexneri 6, and rotaviruses were determined in milk samples obtained serially from women during the first 16 weeks postpartum. the mean concentration of secretory iga followed the expected pattern; the levels of specific antibodies fluctuated in an unpredictable manner and independently of milk secretory iga content, becoming undetectable in many instances. under some circ ...	1985	3004100
relationship of acth1-39-immunostained fibers and magnocellular neurons in the paraventricular nucleus of rat hypothalamus.	dual antigen immunocytochemical staining procedures were used in the same tissue section to determine the distribution of acth immunostained fibers and varicosities within the magnocellular and parvocellular divisions in the paraventricular nucleus (pvn) of rat hypothalamus and elucidate its anatomical relationship to vasopressin (vp) and oxytocin (oxy)-containing neurons. double immunostained preparations using glucose oxidase-antiglucose oxidase complex combined with pap complex to visualize t ...	1985	3001668
characterization of electron transfer and proton translocation activities in trypsin-treated bovine heart mitochondrial cytochrome c oxidase.	bovine heart mitochondrial cytochrome c oxidase has been treated with trypsin in order to investigate the role of components a, b, and c (nomenclature of capaldi) in cytochrome c binding, electron transfer, and proton-pumping activities. cytochrome c oxidase was dispersed in nondenaturing detergent solution (b. ludwig, n. w. downer, and r. a. capaldi (1979) biochemistry 18, 1401) and treated with trypsin. this treatment inhibited electron transfer activity by 9% when compared to a similarly trea ...	1985	3002279
flaviviridae.	the family flaviviridae comprises the genus flavivirus, which contains 65 related species and two possible members. they are small, enveloped rna viruses (diameter 45 nm) with peplomers comprising a single glycoprotein e. other structural proteins are designated c (core) and m (membrane-like). the single strand of rna is infectious and has a molecular weight of about 4 x 10(6) and an m7g 'cap' at the 5' end but no poly(a) tract at the 3' end; it functions as the sole messenger. the gene sequence ...	1985	3000978
characterization of a major protein with a molecular weight of 160,000 associated with the viral capsid of epstein-barr virus.	a monoclonal antibody designated v3 was produced against a late protein associated with the epstein-barr virus-induced viral capsid antigen complex. the antibody reacted with discrete patches in the nuclei of infected cells as well as with virus particles, as shown by immunofluorescence and ultrastructural immunoperoxidase staining. the molecular weight of the protein precipitated by this monoclonal antibody was ca. 160,000. all anti-viral capsid antigen antibody-positive sera tested in an enzym ...	1985	2981328
middle tumor antigen of polyomavirus transformation-defective mutant ng59 is associated with pp60c-src.	we have found that lysis of mouse embryo cells infected with the polyomavirus host range transformation-defective (hr-t) mutant ng59 under gentle conditions that avoid ionic detergents results in detectable ng59-encoded middle tumor antigen (mtag) associated with pp60c-src. this mtag-pp60c-src complex could be immunoprecipitated from ng59-infected cell lysates by either sera from animals bearing polyomavirus-induced tumors or by monoclonal antibodies directed against mtag. immune complex kinase ...	1985	2981329
genome organization of herpesvirus aotus type 2.	herpesvirus aotus type 2, a virus commonly found in owl monkeys without overt disease, has a similar genome structure to the oncogenic herpesviruses of nonhuman primates (herpesvirus saimiri, herpesvirus ateles). virion dna of herpesvirus aotus type 2 (m-dna) has an unique 110-kilobase-pair region of low g + c content (40.2%, l-dna), inserted between stretches of repetitive h-dna (68.7% g + c, about 41 kilobase pairs per molecule) that are variable in length. a minority of virions contain defect ...	1985	2981331
autoregulation of the bacteriophage p22 scaffolding protein gene.	during the formation of each bacteriophage p22 head, about 250 molecules of the product of gene 8, scaffolding protein, coassemble with and dictate correct assembly of the coat protein into a proper shell structure. at approximately the time that dna is inserted inside the coat protein shell, all of the scaffolding protein molecules leave the structure. they remain active and participate in several subsequent rounds of shell assembly. previous work has shown that scaffolding protein gene express ...	1985	2981337
analysis of mobilization elements in plasmids from shigella flexneri.	the mobilization properties of three plasmids were examined after cotransfer from shigella flexneri to escherichia coli. the largest plasmid, pcn1, was shown to be a conjugative r factor that could promote its own transfer and allow cotransfer of a 4.1-kilobase plasmid, pcn3; mobilization of the third plasmid, pcn2 (6.3 kilobases), required the presence of both pcn1 and pcn3. sequences from pcn2 and pcn3 homologous to the bom (basis of mobilization) sites of cole1 and pbr322 were localized by an ...	1985	2982788
adenine nucleotides directly stimulate pertussis toxin.	both cholera toxin and pertussis toxin catalyzed adp-ribosylation of purified bovine brain tubulin. the effect of cholera toxin was evident in the absence or presence of nucleotides. in contrast, pertussis toxin required adenine nucleotides for its adp-ribosylating activity. atp, atp gamma s, app(nh)p, deoxy-atp, and adp all supported pertussis toxin-catalyzed adp-ribosylations in the absence or presence of edta, suggesting that nucleotide hydrolysis was not involved. adenine nucleotides also pr ...	1985	2982826
a new beta-lactamase plasmid in neisseria gonorrhoeae.		1985	2984155
escherichia coli 23s ribosomal rna truncated at its 5' terminus.	in a strain of e. coli deficient in rnase iii (abl1), 23s rrna has been shown to be present in incompletely processed form with extra nucleotides at both the 5' and 3' ends (king et al., 1984, proc. natl. acad. sci. u.s. 81, 185-188). rna molecules with four different termini at the 5' end are observed in vivo, and are all found in polysomes. the shortest of these ("c3") is four nucleotides shorter than the accepted mature terminus. in growing cells of both wild-type and mutant strains up to 10% ...	1985	2987818
opioid modulation of ingestive behaviors in woodchucks and racoons.	we have examined the effect on feeding of opioid blockade with naloxone in two species which demonstrate a marked seasonality in their feeding patterns, the racoon (procyon lotor) and the woodchuck (marmota monax). naloxone suppressed food intake in the woodchuck which is a true hibernator. naloxone failed to suppress food intake in the racoon and, in fact, enhanced intake of a preferred sucrose solution. in the racoon, ir-dynorphine concentrations were extremely high in the hypothalamus compare ...	1985	2987986
isolation of retinoic acid-resistant clones from human breast cancer cell line mcf-7 with altered activity of cellular retinoic acid-binding protein.	after ethyl methane sulfonate mutagenesis of the mammary carcinoma cell line, mcf-7, we have isolated three clones, u-2, u-3, and u-9, resistant to retinoic acid. these three clones showed more than a 1000-fold higher level of resistance to retinoic acid than the parental mcf-7 cells when assayed by colony formation in monolayer culture system or by growth curves. the three resistant clones showed a 200-fold higher resistance to 13-cis-retinoic acid, about 10-fold higher resistance to retinol, a ...	1985	2988768