Publications

isolation of yac clones from the pericentromeric region of chromosome 10 and development of new genetic markers linked to the multiple endocrine neoplasia type 2a gene.	genetic linkage mapping and contig assembly using yeast artificial chromosome (yac) technology form the basis of our strategy to clone and define the genomic structure of the pericentromeric region of chromosome 10 containing the multiple endocrine neoplasia type 2a gene. thus far yac walks have been initiated from five chromosome 10 pericentromeric loci including rbp3, d10s94, ret, d10z1, and fnrb. long range pulsed-field gel electrophoresis maps are constructed from the yacs isolated to define ...	1992	1362407
mapping psychiatric disease genes: impact of new molecular strategies.	genetic mapping of genes which predispose to psychiatric illness is discussed in relation to recent developments in molecular genetic technology. among the psychiatric disorders, the mechanism by which genetic factors contribute to illness is poorly understood, and the classification of phenotype (ill-status) is extremely complicated. these uncertainties, together with other complicating factors, tend to undermine the effectiveness of genetic linkage analysis. two very powerful new molecular str ...	1992	1362776
molecular dissection of the prader-willi/angelman syndrome region (15q11-13) by yac cloning and fish analysis.	prader-willi syndrome (pws) and angelman syndrome (as) are distinct mental retardation disorders associated with deletions of proximal 15q (q11-q13) of different parental origin. yeast artificial chromosome (yac) clones were isolated for 9 previously mapped dna probes from this region, and for one newly derived marker, ls6-1 (d15s113). a yac contig of 1-1.5 mb encompassing four markers (ml34, ir4-3r, pw71, and td189-1) was constructed. multi-color fluorescence in situ hybridization (fish) analys ...	1992	1363801
eukaryotic dna replication.	the past decade has witnessed an exciting evolution in our understanding of eukaryotic dna replication at the molecular level. progress has been particularly rapid within the last few years due to the convergence of research on a variety of cell types, from yeast to human, encompassing disciplines ranging from clinical immunology to the molecular biology of viruses. new eukaryotic dna replicases and accessory proteins have been purified and characterized, and some have been cloned and sequenced. ...	1992	1727692
a protective monoclonal antibody specifically recognizes and alters the catalytic activity of schistosome triose-phosphate isomerase.	mab m.1 was previously shown to recognize a 28-kda ag in all stages of the human helminth parasite, schistosoma mansoni, and to bind to the surface membranes of newly transformed schistosomula in a transient manner. here we demonstrate that m.1 passively transfers partial resistance (41-49%) to cercarial challenge in naive mice. thus, the 28-kda ag recognized by m.1 is a putative vaccine candidate. after immunoaffinity purification, tryptic digests of the 28-kda ag were prepared and individual p ...	1992	1729373
yeast artificial chromosomes spanning 8 megabases and 10-15 centimorgans of human cytogenetic band xq26.	a successful test is reported to generate long-range contiguous coverage of dna from a human cytogenetic band in overlapping yeast artificial chromosomes (yacs). seed yacs in band xq26 were recovered from a targeted library of clones from xq24-q28 with 14 probes, including probes for the hypoxanthine guanine phosphoribosyltransferase- and coagulation factor ix-encoding genes and nine probes used in linkage mapping. neighboring yacs were then identified by 25 "walking" steps with end-clones, and ...	1992	1729687
class ii (b) general transcription factor (tfiib) that binds to the template-committed preinitiation complex is different from general transcription factor btf3.	a class ii (b) general transcription factor of 34 kda has been purified from hela cells to apparent homogeneity. this factor appears to be transcription factor iib (tfiib), since it binds in vitro to template-committed preinitiation complexes formed between a template containing the tata box/cap-site elements of the adenovirus type 2 major late promoter (ad2mlp) and recombinant human or yeast tfiid (previously called btf1) expressed in escherichia coli. dnase i footprint studies show an extended ...	1992	1729710
saccharomyces cerevisiae elongation factor 2. genetic cloning, characterization of expression, and g-domain modeling.	the elongation factor 2 (ef-2) genes of the yeast saccharomyces cerevisiae have been cloned and characterized with the ultimate goal of gaining a better understanding of the mechanism and control of protein synthesis. two genes (eft1 and eft2) were isolated by screening a bacteriophage lambda yeast genomic dna library with an oligonucleotide probe complementary to the domain of ef-2 that contains diphthamide, the unique posttranslationally modified histidine that is specifically adp-ribosylated ...	1992	1730643
s-phase feedback control in budding yeast independent of tyrosine phosphorylation of p34cdc28.	in somatic cells, entry into mitosis depends on the completion of dna synthesis. this dependency is established by s-phase feedback controls that arrest cell division when damaged or unreplicated dna is present. in the fission yeast schizosaccharomyces pombe, mutations that interfere with the phosphorylation of tyrosine 15 (y15) of p34cdc2, the protein kinase subunit of maturation promoting factor, accelerate the entry into mitosis and abolish the ability of unreplicated dna to arrest cells in g ...	1992	1731250
marker profile, enzyme activity, and function of a human myelomonocytic leukemia cell line.	morphological and functional characteristics of a permanent human leukemia cell line (dd) that possesses myelomonocytic features were investigated. the cells bear a second type fc gamma receptor and form rosettes with sheep erythrocytes sensitized with rabbit igg (ea). however, the surface-bound ea is not internalized. the cell line lacks the surface markers cd2, cd19, cd14, hla-dr, fc gamma receptor i, fc gamma receptor iii, and cr3. alpha 1-antitrypsin, lysozyme, factor xiii a subunit of blood ...	1992	1733517
transcription factor tfiid induces dna bending upon binding to the tata element.	the tata box-binding factor tfiid plays a primary role in the process of transcription initiation by rna polymerase ii and its regulation by various gene-specific factors. here we employ a permuted binding site/gel retardation assay with recombinant yeast and human tfiid to show that this factor induces dna bending around the tata element. these results are consistent with the presence of g + c-rich sequence elements flanking the consensus tata element and led to the recently confirmed suggestio ...	1992	1736286
contributions of human liver cytochrome p450 enzymes to the n-oxidation of 4,4'-methylene-bis(2-chloroaniline).	4,4'-methylene-bis(2-chloroaniline) (moca) can produce tumors in rodents and dogs and an increased incidence of bladder tumors has been reported in exposed workers. it is therefore of interest to identify the human cytochrome p450 (p450) enzymes involved in moca n-oxidation, the primary reaction involved in the formation of an electrophilic product. human liver microsomes were fractionated and moca n-oxidation activity was monitored through the procedure. the most active enzyme fraction correspo ...	1992	1740010
yeast rad14 and human xeroderma pigmentosum group a dna-repair genes encode homologous proteins.	xeroderma pigmentosum (xp), a human autosomal recessive disorder, is characterized by extreme sensitivity to sunlight and high incidence of skin cancers. xp cells are defective in the incision step of excision repair of dna damaged by ultraviolet light. cell fusion studies have defined seven xp complementation groups, xp-a to xp-g. similar genetic complexity of excision repair is observed in the yeast saccharomyces cerevisiae. mutations in any one of five yeast genes, rad1, rad2, rad3, rad4, and ...	1992	1741034
recognition nucleotides for human phenylalanyl-trna synthetase.	the specificity of the interaction between trnaphe and phenylalanyl-trna synthetase isolated from human placenta was investigated. using yeast trnaphe transcripts with different point mutations it was shown that all the five recognition points for the yeast phenylalanyl-trna synthetase (g20, g34, a35, a36 and a73) are also important for the reaction catalyzed by the human enzyme. a set of mutations in nucleotides involved in tertiary interactions of trnaphe revealed that mutations which maintain ...	1992	1741281
yeast artificial chromosomes (yacs) and the analysis of complex genomes.	the development of yeast artificial chromosome (yac) cloning vectors capable of carrying several hundred kilobase-pairs of dna insert has greatly facilitated the study of complex genomes, and the cloning of large genes as single fragments. in addition, the ability to manipulate yac sequences by homologous recombination makes this system extremely useful for the generation of disease models.	1992	1367930
expression of high levels of human tissue plasminogen activator in yeast under the control of an inducible gal promoter.	the human tissue plasminogen activator (h-tpa) cdna was fused either with the leader sequence of the killer toxin of kluyveromyces lactis or with the saccharomyces diastaticus glucoamylase leader peptide and cloned in the yeast expression vector under the control of the inducible usagal/cyc1 promoter. the recombinant tpa is produced in yeast as a single-chain glycosylated polypeptide of 66-72 kda, which accumulates intracellularly associated with a membrane fraction. using two-step fed-batch fer ...	1992	1368914
protein processing within the secretory pathway.	endoproteolytic cleavage of hormone and neuropeptide precursors, as well as many complex proteins, such as coagulation factors and viral glycoproteins, is a key process in the generation of bioactive polypeptides. these cleavages typically occur at the dibasic amino acid residues lys-arg or arg-arg. the enzymes responsible for the processing belong to a newly discovered family of serine proteases related to the bacterial subtilisins. these include pace (furin), pc1/pc3, pc2 and pace4, which have ...	1992	1368940
characterization of two differently glycosylated molecular species of yeast-derived hepatitis b vaccine carrying the pre-s2 region.	modified hepatitis b virus surface antigen m protein particles (hbsag m-p31c) produced in yeast is mainly composed of two differently glycosylated proteins, gp37 and gp34. gp37 has an n-linked sugar chain and o-linked sugar chains; and gp34 has an n-linked sugar chain bound to the peptide backbone p31. although m-p31c vaccine elicits both anti-s and anti-pre-s2 antibodies, whether there are any differences between gp37 and gp34 in the ability to elicit these antibodies is still unknown. to clari ...	1992	1369148
molecular cloning and evidence for osmoregulation of the delta 1-pyrroline-5-carboxylate reductase (proc) gene in pea (pisum sativum l.).	several cdna clones encoding delta 1-pyrroline-5-carboxylate reductase (p5cr, l-proline:nad[p]+ 5-oxidoreductase, ec 1.5.1.2), which catalyzes the terminal step in proline biosynthesis, were isolated from a pea leaf library screened with a 32p-labeled aval fragment of a soybean nodule p5cr cdna (a.j. delauney, d.p.s. verma [1990] mol gen genet 221: 299-305). dna sequence analysis of one full-length 1.3-kb clone (ppps3) indicated that the pea p5cr gene contains a single major open reading frame ...	1992	11537868
production of a site specifically cleavable p-glycoprotein-beta-galactosidase fusion protein.	we have fused full length and the carboxyl-half of human mdr1 cdna with the e. coli lacz gene via a collagen linker and allowed their expression in yeast saccharomyces cerevisiae. using antibodies against beta-galactosidase we partially purified the fusion proteins by immunoprecipitation and show here that the full length fusion protein has atpase activity. by contrast, the fusion protein containing the carboxyl-half of p-glycoprotein did not show atpase activity, indicating that both domains of ...	1991	1369454
expression of the human blood coagulation protein factor xiiia in saccharomyces cerevisiae: dependence of the expression levels from host-vector systems and medium conditions.	the human blood coagulation protein factor xiiia (fxiiia) was expressed in saccharomyces cerevisiae employing escherichia coli-yeast shuttle vectors based on a 2-mu plasmid. several factors affecting high production yield of recombinant fxiiia were analysed. the use of the regulatable gal-cyc1 hybrid promoter resulted in higher fxiiia expression when compared with the constitutive adci promoter. screening for suitable yeast strains for expression of fxiiia under the transcriptional control of th ...	1991	1369455
expression of human p-glycoprotein in yeast cells--effects of membrane component sterols on the activity of p-glycoprotein.	a human mdr1 cdna was introduced into yeast cells. immunoblot analysis and indirect immunostaining showed that some of the p-glycoprotein produced was situated in its native orientation in the yeast plasma membrane. drug-binding activities of the recombinant p-glycoproteins were markedly decreased compared to that of the authentic p-glycoprotein. to identify the bases of decreased binding we studied the effects of membrane component sterols on the azidopine binding and found that ergosterol, whi ...	1991	1369463
cloning and characterisation of the saccharomyces cerevisiae glycerol-3-phosphate dehydrogenase (gut2) promoter.	the saccharomyces cerevisiae glycerol-3-phosphate dehydrogenase (gut2) promoter and part of the protein-coding region have been isolated on a 6.3-kb genomic dna fragment. nucleotide sequence analysis shows that the promoter has many structural features in common with yeast glycolytic enzyme promoters. chromosomal mapping indicates that this genomic fragment is located on chromosome xii. the gut2 promoter has been used to construct a recombinant human albumin (reha) secretion vector; yeast transf ...	1991	1676389
human vntr sequences in porcine htf-islands.	mapping of complex genomes has been influenced substantially by the isolation of locus-specific, but repetitive dna elements known as vntrs. since a high gc-content is characteristic of most of these elements one might expect them to be clustered at least partially in cpg-islands. to address this question we have constructed a porcine liver dna noti-linkage library in puc18 using isolated htf-islands. hpaii tiny fragments ranging from less than 100 bp to 1 kb in length were randomly selected and ...	1991	1678358
signal transduction and transcriptional regulation of interferon-alpha-stimulated genes.	interferon-alpha (ifn-alpha) stimulates the expression of a number of genes in a pathway that begins with binding to specific high-affinity plasma membrane receptors. all ifn-alpha-stimulated genes cloned thus far are characterized by the presence of a dna element, termed interferon-stimulated response element (isre), usually in the 5' upstream region of the genes. the isre binds a nuclear factor(s) following ifn-receptor triggered signal transduction and provides a convenient assay for the rapi ...	1991	1680934
synthesis of dna by dna polymerase epsilon in vitro.	the isolation of dna polymerase (pol) epsilon from extracts of hela cells is described. the final fractions contained two major subunits of 210 and 50 kda which cosedimented with pol epsilon activity, similar to those described previously (syvaoja, j., and linn, s. (1989) j. biol. chem. 264, 2489-2497). the properties of the human pol epsilon and the yeast pol epsilon were compared. both enzymes elongated singly primed single-stranded circular dna templates. yeast pol epsilon required the presen ...	1991	1682323
failure to detect integrins of the beta-2 subclass on the human sperm surface.	several cell-to-cell recognition systems utilize the tripeptide asp-gly-arg (rgd) as a cell surface signaling sequence. mac-1, a member of the integrin subfamily defined by the beta-2 subunit, recognizes c3bi by means of an rgd sequence. because c3 complement components have been detected on the gamete surfaces and rgd has been demonstrated to be involved in sperm-egg interaction, we explored the possibility that a member of the b2 integrin subfamily was present on the sperm surface and involved ...	1991	1686173
characterization and large production of human monoclonal antibodies against the hiv-1 envelope.	peripheral blood lymphocytes from a volunteer immunized with a recombinant vaccinia virus vsc-25 expressing the gp160 env protein of htlv-iiib strain and from an asymptomatic hiv-infected individual were immortalized by epstein-barr (ebv). clones which secrete human monoclonal antibodies from the two individuals (dz, igg1, lambda and c31, igg1, kappa) were obtained and were stable for more than 2 years. the two monoclonals were directed against the gp160 env protein of hiv, dz directed against t ...	1991	1706239
regulation of leukocyte adhesion molecule-1 (tq1, leu-8) expression and shedding by normal and malignant cells.	the human leukocyte adhesion molecule-1 (lam-1, tq1, leu-8) is involved in the binding of human leukocytes to high endothelial venules (hev) of peripheral lymph nodes (ln). the regulation of lam-1 expression is unique in that leukocyte stimulation induces a rapid down-modulation of lam-1 from the cell surface. in this study, the regulation and function of lam-1 was studied in detail in normal lymphocytes and compared with the lam-1 of malignant leukocytes. modulation of lam-1 from the cell surfa ...	1991	1709244
cloning, sequence and chromosomal location of a mel gene from saccharomyces carlsbergensis ncyc396.	yeast strains producing alpha-galactosidase (alpha gal) are able to use melibiose as a carbon source during growth or fermentation. we cloned a mel gene from saccharomyces carlsbergensis ncyc396 through hybridization to the mel1 gene cloned earlier from saccharomyces cerevisiae var. uvarum. the alpha gal encoded by the newly cloned gene was galactose-inducible as is the alpha gal encoded by mel1. a probable gal4-protein recognition sequence was found in the upstream region of the ncyc396 mel gen ...	1991	1711992
physicochemical and immunological properties of the hepatitis b surface antigen containing the pres2 9 amino acid sequence produced by a recombinant yeast.	the hepatitis b virus surface antigen containing the pres2 nine amino acid sequence produced by a recombinant saccharomyces cerevisiae (yhbsag) was purified and its physicochemical properties were determined. ultrastructurally, the yhbsag was found to be a homogeneous spherical particle with a diameter of 24 +/- 4 nm. the homogeneity of the yhbsag particles was also demonstrated by analyses of their buoyant density and isoelectric point. they consisted of protein (53%), lipid (36%) and carbohydr ...	1991	1712147
[the activation of the transposition cycle ty element by the reverse transcriptase of the human immunodeficiency virus in saccharomyces cerevisiae yeasts].		1991	1716549
cloning of a human cdna encoding a cdc2-related kinase by complementation of a budding yeast cdc28 mutation.	we have cloned two different human cdnas that can complement cdc28 mutations of budding yeast saccharomyces cerevisiae. one corresponds to a gene encoding human p34cdc2 kinase, and the other to a gene (cdk2; cell division kinase) that has not been characterized previously. the cdk2 protein is highly homologous to p34cdc2 kinase (65% identical) and more significantly is homologous to xenopus eg1 kinase (89% identical), suggesting that cdk2 is the human homolog of eg1. the human cdc2 and cdk2 gene ...	1991	1717994
dna.rna helicase activity of rad3 protein of saccharomyces cerevisiae.	the rad3 gene of saccharomyces cerevisiae is required for excision repair of uv-damaged dna and is essential for cell viability. the rad3 protein exhibits a remarkable degree of sequence homology to the human excision repair protein ercc2. the rad3 protein is a single-stranded dna-dependent atpase and a dna helicase capable of denaturing long regions of duplex dna. here, we demonstrate that rad3 also possesses a potent dna.rna helicase activity similar in efficiency to its dna helicase activity. ...	1991	1719538
fungi in megakaryocytes. an unusual manifestation of fungal infection of the bone marrow.	when fungi infect the bone marrow, typically they are associated with granuloma formation and/or necrosis, and the fungi are found within histiocytes or admixed with necrotic debris. recently two bone marrow biopsy specimens were encountered in which fungi were confined to the cytoplasm of megakaryocytes, a finding not previously reported in the literature. the first case was that of a 46-year-old man with pulmonary histoplasmosis and no known immunodeficiency. the second was that of a 38-year-o ...	1991	1719795
primary structure of the catalytic subunit of human dna polymerase delta and chromosomal location of the gene.	the catalytic subunit (mr approximately 124,000) of human dna polymerase delta has been cloned by pcr using poly(a)+ rna from hepg2 cells and primers designed from the amino acid sequence of regions highly conserved between bovine and yeast dna polymerase delta. the human cdna was 3443 nucleotides in length and coded for a polypeptide of 1107 amino acids. the enzyme was 94% identical to bovine dna polymerase delta and contained the numerous highly conserved regions previously observed in the bov ...	1991	1722322
nucleotide sequence of murine pcna: interspecies comparison of the cdna and the 5' flanking region of the gene.	proliferating cell nuclear antigen (pcna) rna levels are regulated by transcription as well as changes in stability, in growing cells. we have cloned the murine pcna cdna and a fragment of the murine pcna gene flanking the transcription initiation site. comparison of the murine deduced amino acid sequence with the pcna sequence from rat, human, drosophila, saccharomyces cerevisiae, and higher plants, reveals extensive homology between species. the homology is likely to be related to the fundamen ...	1991	1726365
importance of conformation on the neutralizing antibody response to hiv-1 gp120.	we have investigated the role of conformation of hiv-1 gp120 on its potential efficacy as a subunit vaccine. the questions that we set out to answer were: 1) are there neutralizing antibodies directed to conformational epitopes in gp120? 2) if so, what is the spectrum of virus isolates neutralized by these antibodies? 3) is a conformationally correct gp120 subunit more effective in the induction of neutralizing antibodies than a denatured subunit? 4) does native gp120 subunit vaccination induce ...	1991	1726963
molecular cloning and characterization of interferon alpha/beta response element binding factors of the murine (2'-5')oligoadenylate synthetase me-12 gene.	seven clones encoding interferon response element binding factors have been isolated from a mouse fibroblast lambda gt11 cdna library by using a 32p end-labeled tandem trimer of the mouse (2'-5')oligoadenylate synthetase gene interferon response element as a probe. clone 16 shares strong similarity (95%) at both dna and amino acid level with yb-1, a human major histocompatibility complex class ii y-box dna-binding protein, and with dbpb, a human epidermal growth factor receptor gene enhancer reg ...	1991	1986360
covalent structure, disulfide bonding, and identification of reactive surface and active site residues of human prostatic acid phosphatase.	the pairing of the half-cysteine residues of human prostatic acid phosphatase was established by proteolytic digestion and analysis of the resulting peptide mixtures by fast atom bombardment mass spectrometry (fab-ms). an independently derived, full length cdna clone was used as the basis for the interpretation of the fab-ms data. the sequence of the native protein is that predicted from the present cdna sequence, except for the carboxyl-terminal end and some possible post-translational deamidat ...	1991	1989985
in situ hybridization to cytogenetic bands of yeast artificial chromosomes covering 50% of human xq24-xq28 dna.	from the collection described by abidi et al., 102 yeast artificial chromosomes (yacs) with human dna inserts more than 300 kb in length were assigned to chromosomal band positions on early metaphase chromosomes by in situ hybridization using the biotin-avidin method. all the yacs hybridized within the xq24-xqter region, supporting the origin of the vast majority of the yacs from single human x-chromosomal sites. with assignments precise to +/- 0.5 bands, yacs were distributed among cytogenetic ...	1991	1990831
relative efficiencies of the bacterial, yeast, and human dna methyltransferases for the repair of o6-methylguanine and o4-methylthymine. suggestive evidence for o4-methylthymine repair by eukaryotic methyltransferases.	the suicidal inactivation mechanism of dna repair methyltransferases (mtases) was exploited to measure the relative efficiencies with which the escherichia coli, human, and saccharomyces cerevisiae dna mtases repair o6-methylguanine (o6meg) and o4-methylthymine (o4met), two of the dna lesions produced by mutagenic and carcinogenic alkylating agents. using chemically synthesized double-stranded 25-base pair oligodeoxynucleotides containing a single o6meg or a single o4met, the concentration of o6 ...	1991	1993655
further observations on the phagocytosis of candida albicans by hamster and human oocytes.	pathogenic yeast, candida albicans, were incubated with hamster and human oocytes for up to 21 hours in order to determine the nature and time course of phagocytosis of these organisms. aliquotes of the interacting cells were taken at various time intervals for electron microscopic examination. some specimens had their zona pellucidae enzymatically removed prior to incubation with yeast, and these specimens showed the most extensive interaction and phagocytosis of candida. the zona pullucida app ...	1991	1994983
p68 rna helicase: identification of a nucleolar form and cloning of related genes containing a conserved intron in yeasts.	the human p68 protein is an rna-dependent atpase and rna helicase which was first identified because of its immunological cross-reaction with a viral rna helicase, simian virus 40 large t antigen. it belongs to a recently discovered family of proteins (dead box proteins) that share extensive regions of amino acid sequence homology, are ubiquitous in living organisms, and are involved in many aspects of rna metabolism, including splicing, translation, and ribosome assembly. we have shown by immun ...	1991	1996094
conversion of pig pancreas phospholipase a2 by protein engineering into enzyme active against escherichia coli treated with the bactericidal/permeability-increasing protein.	phospholipases a2 (pla-2) are conserved enzymes that can vary widely in their activity toward certain biological targets. activity of pla-2 toward escherichia coli treated with the bactericidal/permeability-increasing protein (bpi) of granulocytes has been detected only in "group ii" pla-2 (lacking cys11-cys77) and correlates with overall basicity and the presence of a cluster of basic amino acids within a variable surface region near the nh2 terminus (including residues 6, 7, 10, 11, and 15). w ...	1991	1999411
effect of soluble aminated beta-1,3-d-polyglucose on human monocytes: stimulation of cytokine and prostaglandin e2 production but not antigen-presenting function.	glucans are insoluble polymers of beta-1,3-linked glucose derived from yeast cell walls that effectively activate macrophages. recently, aminated derivatives of beta-1,3-d-polyglucose have been developed that are soluble but also activate murine macrophages. the current studies were undertaken to determine whether soluble aminated beta-1,3-d-polyglucose (ag) would also stimulate human monocytes. the ag employed contained less than 2 ng endotoxin/mg. ag induced the production of intracellular, me ...	1991	2002282
properties and applications of human dna repair genes.	the importance of understanding dna repair processes is discussed in terms of the origins of human cancer. several human repair genes have been mapped to specific human chromosomes using somatic cell hybrids. it is noteworthy that 3 of these genes lie in the same region of chromosome 19: genes ercc1 and ercc2, which are involved in nucleotide excision repair, and xrcc1, which is involved in the repair of strand breaks. the genes xrcc1 and ercc2 were cloned from cosmid libraries prepared from dna ...	1991	2011139
structural and transcriptional analysis of a human subtelomeric repeat.	a human subtelomeric repeat (designated as the hst repeat) has been isolated and characterized from a yeast artificial chromosome containing one human telomere. this repeat is located immediately adjacent to the telomeric t2ag3 repeats at the extreme termini of the human chromosomes. the dna sequence of 3.6 kb of the hst repeat has been determined. the hst repeat spans over 3.6 kb in length, and contains one evolutionarily conserved cpg-rich region. the copy number of the hst repeat varies among ...	1991	2011494
genetic destabilization of candida albicans by hydroxyurea.	candida albicans is a commensal component of the normal human microflora, but frequently causes infections in persons undergoing treatment for malignancies. the cytotoxicity and genotoxicity of the antineoplastic agent hydroxyurea (hu) for the yeast is demonstrated. cultivation of the organism on defined complete medium in the presence of hu induced growth inhibition or cell death, gene mutations, segregations of heterozygous loci through reciprocal and nonreciprocal mitotic recombinations, and ...	1991	2014007
a highly conserved domain of tfiid displays species specificity in vivo.	recombinant tfiid proteins from yeast, drosophila, and human function interchangeably in vitro to restore basal level transcription to a human hela extract depleted for tfiid. here we report that the recently cloned human and drosophila tfiid genes fail to substitute in vivo for the s. cerevisiae tfiid gene, spt15, which is essential for viability. analysis of yeast-human hybrid tfiid proteins reveals that the failure of human tfiid to functionally replace yeast tfiid maps to the highly conserve ...	1991	2015627
mammalian camp-dependent protein kinase functionally replaces its homolog in yeast.	the cdna encoding the catalytic subunit (c alpha) from mouse camp-dependent protein kinase (pk) was expressed in saccharomyces cerevisiae. by a plasmid swap procedure, we demonstrated that the mammalian c alpha subunit can functionally replace its yeast homolog to maintain the viability of a yeast strain containing genetic disruptions of the three tpk genes encoding the yeast c subunits. c alpha subunit produced in yeast was purified and its biochemical properties were determined. the protein is ...	1991	2022331
construction and characterization of a partial library of yeast artificial chromosomes from human chromosome 21.	we report a protocol for cloning large dna fragments in yeast artificial chromosomes (yac). a partial library has been constructed from a somatic hybrid containing chromosome 21 as the single source of human dna. about 4.0 mb of human dna was recovered in 17 yac clones. three clones were analyzed by in situ hybridization and mapped on chromosome 21. one clone hybridized with the chromosome 21 centromeric region and may provide new insight both on the molecular structure of centromere and on the ...	1991	2029338
escherichia coli dna polymerase ii is homologous to alpha-like dna polymerases.	the escherichia coli polb gene encodes dna polymerase ii and is regulated by the sos system. we sequenced a 4081 nucleotide segment of the e. coli chromosome that contains the polb gene and its flanking regions. dna polymerase ii, as deduced from the dna sequence, consists of 782 amino acids, has a molecular weight of 89,917, and is structurally homologous to alpha-like dna polymerases, which include eukaryotic replicative dna polymerases. comparison of the sequences of the alpha-like dna polyme ...	1991	2034216
disseminated histoplasmosis with bilateral adrenal enlargement: diagnosis by computed tomography-directed needle biopsy.	disseminated histoplasmosis is a rare and potentially fatal disease caused by the dimorphic soil fungus histoplasma capsulatum. we describe a 67-year-old man with diabetes who presented with a 6-month history of fever, weight loss, and mental deterioration; using computed tomography (ct), we found hepatosplenomegaly and bilateral adrenal masses. ct-directed adrenal biopsy showed yeast forms consistent with h capsulatum. therapy with ketoconazole was accompanied by adrenal insufficiency (treated ...	1991	2035163
the human hprt gene on a yeast artificial chromosome is functional when transferred to mouse cells by cell fusion.	a 680-kb yeast artificial chromosome (yac) that contains a functional copy of the human hypoxanthine phosphoribosyltransferase (hprt) gene has been isolated. this yac, yhprt, and another yac, yxy837, which contains the 3' end of the hprt gene, have been mapped with restriction enzymes that cleave human dna infrequently. the hprt gene lies near the center of yhprt. fusion of yhprt-containing yeast spheroplasts with mouse l a-9 cells, which are hprt-negative, gives rise to hprt-positive colonies. ...	1991	2037299
the cytosolic and glycosomal glyceraldehyde-3-phosphate dehydrogenase from trypanosoma brucei. kinetic properties and comparison with homologous enzymes.	the protozoan haemoflagellate trypanosoma brucei has two nad-dependent glyceraldehyde-3-phosphate dehydrogenase isoenzymes, each with a different localization within the cell. one isoenzyme is found in the cytosol, as in other eukaryotes, while the other is found in the glycosome, a microbody-like organelle that fulfils an essential role in glycolysis. the kinetic properties of the purified glycosomal and cytosolic isoenzymes were compared with homologous enzymes from other organisms. both trypa ...	1991	2040304
behavior of cysteine mutants of human lysozyme in de novo synthesis and in vivo secretion.	to investigate the mechanism of disulfide-bond-coupled de novo folding of human lysozyme, we have constructed 23 mutant enzymes in which cysteine residue(s) were replaced by alanine(s). the mutant genes were translated in vitro in a system composed of rabbit reticulocyte lysate, canine pancreatic microsomal vesicles and oxidized glutathione. this system allows the formation of intramolecular disulfide bonds in translation products translocated into the microsomal lumen. the mobilities of the tra ...	1991	2040307
molecular mimicry in candida albicans. role of an integrin analogue in adhesion of the yeast to human endothelium.	hematogenous infection with the yeast candida albicans now occurs with increasing frequency in the neonate, the immunocompromised patient, and the hyperglycemic or hyperalimented host. yeast-phase c. albicans expresses a protein that is antigenically and structurally related to cd11b/cd18, a member of the beta 2 integrins and a well-characterized adhesin for mammalian neutrophils. both the neutrophil protein and its analogue in c. albicans have an identical affinity for the c3 ligand ic3b, and b ...	1991	2040685
identification of lanosterol 14 alpha-methyl demethylase in human tissues.	lanosterol 14 alpha-methyl demethylase was investigated in human tissues using a radio-hplc assay to detect the 4,4-dimethyl-5 alpha-cholesta-8, 14-dien-3 beta-ol (diene) metabolite. the sequence of events leading to the demethylated product in human liver microsomes involves the conversion of the diol to the aldehyde followed by diene formation. enzyme activity displayed a greater than 10 fold variation among the 9 liver samples studied. kinetic parameters were determined and shown to differ be ...	1991	2043135
one-sided action of amphotericin b on cholesterol-containing membranes is determined by its self-association in the medium.	the inducement of k+ permeability through membranes by the polyene antibiotic amphotericin b (amb) has been analyzed as a measure of the antibiotic activity. dose-response curves have been obtained with cholesterol- and ergosterol-containing egg yolk phosphatidylcholine large unilamellar vesicles (luvs), human erythrocytes, and saccharomyces cerevisiae cells. conductance changes induced by amb in sterol-containing planar bilayer membranes have also been studied. amb self-association in aqueous b ...	1991	2043613
cloning and in vivo expression of the human gart gene using yeast artificial chromosomes.	two yeast artificial chromosomes (yacs) were isolated each with a full-length copy of the human gene that encodes the trifunctional protein containing phosphoribosylglycinamide synthetase (gars), phosphoribosylglycinamide formyltransferase (gart) and phosphoribosylaminoimidazole synthetase (airs). the yacs were characterized by restriction mapping and by in situ hybridization of cosmid subclones containing the yac ends to human metaphase chromosomes. one of the yacs contains co-cloned non-contig ...	1991	2050105
characterization of a cdna encoding the 70-kda single-stranded dna-binding subunit of human replication protein a and the role of the protein in dna replication.	replication protein a (rp-a) is a three-subunit single-stranded dna-binding protein that has been isolated from human cells. rp-a is essential for sv40 dna replication and may also be important in genetic recombination. the sequence of a cdna encoding the 70-kda subunit of human rp-a is reported. the 616-amino acid predicted open reading frame of the human protein is 31% identical with the 621-amino acid open reading frame of the 70-kda subunit of rp-a from the yeast saccharomyces cerevisiae. bo ...	1991	2050703
isolation of a yeast artificial chromosome spanning the 8;21 translocation breakpoint t(8;21)(q22;q22.3) in acute myelogenous leukemia.	the 8;21 translocation is one of the most common specific rearrangements in acute myelogenous leukemia. we have identified markers (d21s65 and a not i boundary clone, not-42, referred to as probe b) flanking the chromosome 21 translocation breakpoint (21q22.3) that demonstrate physical linkage in normal genomic dna, by using at least three restriction endonucleases (not i, sac ii, and bsshii), and that are located not more than 250-280 kilobases apart. pulsed-field gel analysis of dna from somat ...	1991	2052570
source and role of diacylglycerol formed during phagocytosis of opsonized yeast particles and associated respiratory burst in human neutrophils.	the results presented in this paper demonstrate that in human neutrophils phagocytosis of c3b/bi and igg-opsonized yeast particles is associated with activation of phospholipase d and that this reaction is the main source of diglycerides. the demonstration is based upon the following findings: 1) the challenge of neutrophils with these opsonized particles was followed by a rapid formation of [3h]alkyl-phosphatidic acid [( 3h]alkyl-pa) and [3h]alkyl-diglyceride [( 3h]alkyl-dg) in cells labeled wi ...	1991	2059222
a region of proto-dbl essential for its transforming activity shows sequence similarity to a yeast cell cycle gene, cdc24, and the human breakpoint cluster gene, bcr.	proto-dbl is a human proto-oncogene, whose oncogenic activation was initially detected by dna transfection. we report significant sequence similarity between the predicted proto-dbl product and the products of cdc24, a saccharomyces cerevisiae cell division cycle gene required for correct budding and establishment of cell polarity, and bcr, a gene implicated in the pathogenesis of chronic myelogenous leukemia (cml). of 925 residues of the predicted proto-dbl protein, a stretch of 238 residues sh ...	1991	2065022
affinity purification of transcription factor iia from hela cell nuclear extracts.	one of the general transcription factors, tfiia, was purified to homogeneity from hela cell nuclear extracts by yeast tfiid affinity chromatography. human tfiia had a molecular weight of approximately 38 kd. it was able to associate with the complex formed by yeast tfiid and the tata elements of the adenovirus e4 and ml promoters, and the hsp70 promoter. the association extended the protected region on each tata element by yeast tfiid from dnase i digestion. affinity-purified tfiia was also able ...	1991	2065666
alu-primed polymerase chain reaction for regional assignment of 110 yeast artificial chromosome clones from the human x chromosome: identification of clones associated with a disease locus.	over 400 yeast artificial chromosome (yac) clones were isolated from the human x chromosome, and 110 of these were assigned to regions defined by chromosome translocation and deletion breakpoints. polymerase chain reaction using alu primers was applied to yac clones in order to generate probes, to identify overlapping clones, and to derive "fingerprints" and sequence data directly from total yeast dna. several clones were identified in regions of medical interest. one set of three overlapping cl ...	1991	2068096
striking structural and functional similarities suggest that intestinal sucrase-isomaltase, human lysosomal alpha-glucosidase and schwanniomyces occidentalis glucoamylase are derived from a common ancestral gene.	sequence comparison of the primary structure of the yeast schwanniomyces occidentalis glucoamylase (gam) with gams in different microorganisms did not reveal significant similarities. by contrast, striking similarities were, surprisingly, found with 3 mammalian secretory and integral membrane proteins: the 2 subunits of intestinal brush border sucrase-isomaltase and human lysosomal alpha-glucosidase. the similarities among these proteins are found as clusters of up to 8 amino acids and distribut ...	1991	1743281
coactivators for a proline-rich activator purified from the multisubunit human tfiid complex.	the mechanisms of transcriptional activation directed by sequence-specific regulators is central to understanding gene regulation. here, we report the isolation of coactivators responsible for mediating transcriptional activation by gal4-pro, a hybrid regulator containing the proline-rich activation domain of human ctf/nfi. chromatographic studies indicate that endogenous human tfiid consists of a multisubunit complex containing the tata-binding protein (tbp), coactivators, and other associated ...	1991	1748279
purification, characterization, cloning, and amino acid sequence of the bifunctional enzyme 5,10-methylenetetrahydrofolate dehydrogenase/5,10-methenyltetrahydrofolate cyclohydrolase from escherichia coli.	we have purified the enzyme 5,10-methylenetetrahydrofolate dehydrogenase (ec 1.5.1.5) from escherichia coli to homogeneity by a newly devised procedure. the enzyme has been purified at least 2,000-fold in a 31% yield. the specific activity of the enzyme obtained is 7.4 times greater than any previous preparation from this source. the purified enzyme is specific for nadp. the protein also contains 5,10-methenyltetrahydrofolate cyclohydrolase (ec 3.5.4.9) activity. sodium dodecyl sulfate-polyacryl ...	1991	1748668
transfer of yeast artificial chromosomes from yeast to mammalian cells.	human dna can be cloned as yeast artificial chromosomes (yacs), each of which contains several hundred kilobases of human dna. this dna can be manipulated in the yeast host using homologous recombination and yeast selectable markers. in relatively few steps it is possible to make virtually any change in the cloned human dna from single base pair changes to deletions and insertions. in order to study the function of the cloned dna and the effects of the changes made in the yeast, the human dna mu ...	1991	1755830
dna sequences of chromosome 21-specific yac detect the t(8;21) breakpoint of acute myelogenous leukemia.	the t(8;21)(q22;q22) is a nonrandom translocation specifically marking blasts of acute myelogenous leukemia (aml) with undifferentiated phenotype. the breakpoint on chromosome 21 involved by this rearrangement has been precisely localized relative to cloned dna markers by physical and genetic linkage analysis enabling the use of positional cloning for its isolation. yeast artificial chromosome (yac) clones for loci proximal (d21s65) and distal (erg) to the (21q22) breakpoint have been developed ...	1991	1756477
[cryptococcal meningitis in aids: successful long-term prophylaxis with fluconazole].	a 30-year-old, hiv-positive, man who had been repeatedly treated with amphotericin b for oral thrush, developed headaches, fever up to 38.5 degrees c, dizzy spells with falling tendency, as well as disorder of speech and word finding. cerebrospinal fluid (csf) contained 5700/3 cells, of which 90% were encapsulated yeast-fungus. cryptococcal antigen titres were elevated both in serum (1:256) and csf (1:1024), providing the diagnosis of cryptococcal meningitis. intravenous treatment was started wi ...	1991	1756695
variability in commercial histoplasma complement fixation antigens.	using immuno-mycologics (immy; norman, okla.) histoplasmal yeast (hy) and mycelial (hm) antibody complement fixation test antigens, we retested 1,386 samples that were initially tested with meridian diagnostics, inc. (cincinnati, ohio), antigens. histoplasma antibody was identified (greater than or equal to 1:16) in 20% of hy and 5% of hm samples reported to have titers of less than 1:8 with meridian reagents. immy titers were at least fourfold higher than meridian titers in 39% of hy and 54% of ...	1991	1761695
dna polymerase delta: gene sequences from plasmodium falciparum indicate that this enzyme is more highly conserved than dna polymerase alpha.	genes encoding proteins homologous to the catalytic subunits of dna polymerase alpha and delta have been cloned from the human malaria parasite plasmodium falciparum. these are among the first cellular replicative dna polymerase genes to be cloned and their sequences allow us to make new statements about the relative degrees of conservation of these two enzymes. the most important finding was that p. falciparum pol delta showed considerable homology to the only other pol delta enzyme for which p ...	1991	1762904
molecular linkage of the hla-dr, hla-dq, and hla-do genes in yeast artificial chromosomes.	eight major histocompatibility complex (mhc) class ii loci and the newly defined y3/ring 4 locus were isolated in overlapping yeast artificial chromosome (yac) clones defining a 420-kb segment of human chromosome 6p21.3. yac b1d12 spanning 320 kb contained seven of these loci from hla-dra to hla-dqb2. a 330-kb yac, a148a7, spanned from the hla-dqa1 locus through the y3/ring 4 locus and extended at least 130 kb centromeric of yac b1d12. southern blotting demonstrated that yac b1d12 derived from t ...	1991	1774062
application of an indirect immunofluorescence test for detection of mycoplasma pneumoniae in respiratory exudates.	we prepared polyclonal antibody specific to mycoplasma pneumoniae and examined the conditions influencing the ability of an indirect immunofluorescence test to detect the specific antigen in respiratory exudates. the antibody did not cross-react with normal human serum or with respiratory exudates from 10 healthy persons. cross-reactivity of the antibody with species of mycoplasmas other than m. genitalium was fully diminished when absorbed with horse serum and yeast extract, components of the c ...	1991	1774328
nucleotide sequence of a human heart cdna encoding the mitochondrial phosphate carrier.	we have isolated and characterized a full length cdna clone encoding the precursor of the human heart mitochondrial phosphate carrier protein. the entire clone is 1330 bp in length with 5'- and 3'-untranslated regions of 48 and 184 bp, respectively. the open reading frame encodes the mature protein consisting of 312 amino acids, preceded by a presequence of 49 amino acids. the amino acid sequence of the mature human phosphate carrier is 93.6, 94.2 and 33.6% identical to that of the phosphate car ...	1991	1777677
structure comparison and evolutionary relations between elongation factors ef-tu (ef-1 alpha) and sup 2 proteins.	on the basis of high homology and structural similarity, three genes, sup2 saccharomyces cerevisiae, sup2 pichia pinus and gst1 homo sapiens, might be considered as members of one family named sup2. comparison of the primary structure of sup2 proteins and elongation factors ef-tu(ef-1 alpha) from 19 different species was performed. it was found that sup2 proteins bear more homology to eukaryotic elongation factor than to procaryotic ef-tu, though the degree of sequence conservation in sup2 prote ...	1991	1778473
genetic and physical mapping around the properdin p gene.	a ca repeat has been found on the human x chromosome within 16 kb of the gene encoding properdin p factor (pfc) and has been shown to be a highly informative marker. two more polymorphic ca repeats were found in a cosmid containing dxs228. the ca repeats, and other markers from proximal xp, were mapped genetically in ceph families and the likely order of markers was established as xpter-(dxs7, mao-a, dxs228)-(pfc, dxs426)-(timp, oatl1)-dxs255-xcen. this places pfc in the region xp11.3-xp11.23, t ...	1991	1783405
[the occurrence of cryptococcus neoformans in fecal samples from birds kept in human living areas].	with help of guizotia creatinine agar (syn. bird seed agar) cryptococcus (cr.) neoformans var. neoformans was isolated from 71 (7.7%) of 925 investigated droppings of birds kept within human living area. cr. neoformans was detected in droppings of 1.2% from 164 psittacines, in droppings of 1.7% from 118 budgerigars and in droppings of 18.4% from (carrier) pigeons. this yeast was not isolated from droppings of 13 small parrots, 10 eclectus parrots, 13 rosellas, 21 cockatiels, 6 polytelidus sp., 3 ...	1991	1786044
assessment of lymphocyte and phagocytic functions in goats treated with glucan.	the effect of glucan, a biological response modifier of yeast origin, on different immune functions was studied after the intramuscular application in goats. the simultaneous administration of glucan with human serum albumin or tetanus toxoid significantly stimulated the antibody production to both antigens differing in their thymus dependency. similarly, the phagocytizing activity of the blood polymorphonuclear leukocytes measured by the reductase colorimetric assay significantly increased one ...	1991	1789024
prohormone-converting enzymes: regulation and evaluation of function using antisense rna.	several putative peptide-processing endoproteases have been identified by homology to the yeast kex2 endoprotease, including furin, pc2, and pc1. however, the question is still open as to which might be involved in peptide posttranslational processing. to enable detailed comparisons of physiological changes in peptide processing with biochemical and molecular biological studies, we cloned rat pituitary cdnas for pc1 and pc2. the amino acid sequence homologies among rat, human, and mouse pc1, pc2 ...	1991	1791845
1h and 15n nmr study of human lysozyme.	the 15n signal assignment of human lysozyme was carried out by using 1h-1h and 1h-15n two dimensional experiments. to solve the severe overlap problem of the nh signals, uniform labeling of the protein with 15n was introduced. the uniformly 15n labeled protein was prepared using a high-expression system of saccharomyces cerevisiae. from the analyses of 1h and 15n nmr spectra, all of the backbone 15n signals of the molecule were assigned to each specific residue in the amino acid sequence. recent ...	1991	1794972
stimulation of human neutrophil phagocytosis of candida albicans by a beta-glucan synthase inhibitor, cilofungin.	the effect of a lipopeptide antifungal agent, cilofungin, on serum opsonization and phagocytosis of candida albicans yeast phase cells in human neutrophil monolayer assays was investigated. simultaneous addition of fungicidal concentrations of cilofungin did not enhance or inhibit phagocytosis of c. albicans. pretreatment of candida blastospores with cilofungin in the absence of serum complement for 1 h did not affect phagocytosis. however, pretreatment of blastospores with cilofungin and comple ...	1991	1797051
generation of 5-fluorouracil from 5-fluorocytosine by monoclonal antibody-cytosine deaminase conjugates.	cytosine deaminase (cdase) catalyzes the conversion of cytosine to uracil and is also able to convert the clinically used antifungal agent 5-fluorocytosine (5fc) into the anticancer drug 5-fluorouracil (5fu). the enzyme was purified from bakers' yeast in a six-step procedure. studies indicated that bakers' yeast cdase had a molecular weight of approximately 32 kda and was composed of two subunits of equal molecular weights. monoclonal antibodies were covalently attached to cdase, forming conjuga ...	1991	1805942
[regulation of ca(2+)-dsrna for proliferation and terminal differentiation processes of human fibroblasts and hela cells].	ca2+ complexes of dsrna, poly(da) and poly(dt) of yeast low molecular weight rna produce a pronounced mitogenic effect on human fibroblasts at early stages of fibroblast proliferation in culture. at later stages of cell cultivation ca(2+)-dsrna stimulates terminal differentiation by inducing the synthesis of proteins characteristic of the postmitotic population of human fibroblasts undergoing terminal differentiation. ca(2+)-dsrna produces a stimulating effect on c-fos and c-jun gene transcripti ...	1991	1805979
ultrastructural changes induced by alpha-sarcin in a human pulmonary tumor grown in naked mice.	alpha-sarcin is a cytotoxic polypeptide produced by aspergillus giganteus. it suppresses protein synthesis in yeast and wheat germ extracts and has a purine-specific rnase activity. the substance has been tested for its antitumor properties in a series of induced tumor systems in mice such as sarcoma and carcinoma among others. although some of the in vitro effects of alpha-sarcin on certain cellular components have been elucidated, the biological effects leading to cellular damage are still obs ...	1991	1806047
yeast artificial chromosome (yac) clones and sequence tagged site (sts)markers anchored at human chromosome 21.		1991	1811099
partial purification of a mannosyltransferase involved in the o-mannosylation of glycoproteins from saccharomyces cerevisiae.	the mannosyltransferase that catalyses the transfer of mannose from dolichyl-phosphate-mannose (dol-p-man) to the hydroxyl group of serine/threonine residues in the acceptor peptide (tyr-asn-pro-thr-ser-val) was partially purified approximately 150-fold from the microsomal membrane fraction of saccharomyces cerevisiae. the membrane-bound enzyme was solubilized with 0.5% triton x-100 at a protein:detergent ratio of 2:1, and was then purified by ion-exchange chromatography on deae-cellulose, follo ...	1991	1820198
pas1, a yeast gene required for peroxisome biogenesis, encodes a member of a novel family of putative atpases.	pas genes are required for peroxisome biogenesis in the yeast s. cerevisiae. here we describe the cloning, sequencing, and characterization of the pas1 gene. its gene product, pas1p, has been identified as a rather hydrophilic 117 kd polypeptide. the predicted pas1p sequence contains two putative atp-binding sites and reveals a structural relationship to three other groups of proteins associated with different biological processes such as vesicle-mediated protein transport (nsf and sec18p), cont ...	1991	1825027
a novel cyclin encoded by a bcl1-linked candidate oncogene.	we have previously identified a candidate oncogene (prad1 or d11s287e) on chromosome 11q13 which is clonally rearranged with the parathyroid hormone locus in a subset of benign parathyroid tumours. we now report that a cloned human placental prad1 complementary dna encodes a protein of 295 amino acids with sequence similarities to the cyclins. cyclins can form a complex with and activate p34cdc2 protein kinase, thereby regulating progress through the cell cycle. prad 1 messenger rna levels vary ...	1991	1826542
annexin iii translocates to the periphagosomal region when neutrophils ingest opsonized yeast.	after phagocytosis, killing and digestion of ingested microorganisms depend on fusion of phagocytic vesicle membranes with membranes of intracellular vesicles (azurophil and specific granules). there is considerable evidence that phagosome-granule membrane fusion is regulated by transient increases in intracellular ionized ca2+. in previous studies, we found that a cytosolic ca2(+)-dependent membrane-binding protein, annexin iii, represents over 1% of the total protein of human neutrophils and p ...	1991	1826705
dephosphorylation and activation of a p34cdc2/cyclin b complex in vitro by human cdc25 protein.	oocytes arrested in the g2 phase of the cell cycle contain a p34cdc2/cyclin b complex which is kept in an inactive form by phosphorylation of its p34cdc2 subunit on tyrosine, threonine and perhaps serine residues. the phosphatase(s) involved in p34cdc2 dephosphorylation is unknown, but the product of the fission yeast cdc25+ gene, and its homologues in budding yeast and drosophila are probably positive regulators of the transition from g2 to m phase. we have purified the inactive p34cdc2/cyclin ...	1991	1828290
direct interaction between adenovirus e1a protein and the tata box binding transcription factor iid.	adenovirus e1a has long been known to activate/repress cellular and viral transcription. the transcriptional activity of nuclear extracts was depleted after chromatography on immobilized e1a protein columns that specifically retained the transcription factor (tf) iid. stronger direct interactions between e1a and human tfiid than between e1a and yeast tfiid suggest that the unique sequences of the human protein may be involved. we have demonstrated that this interaction occurs directly between ba ...	1991	1828892
both oct-1 and oct-2a contain domains which can activate the ubiquitously expressed u2 snrna genes.	the u2 snrna genes, which are transcribed by rna polymerase ii at high levels in all tissues examined, require both a distal and a proximal sequence element for efficient expression. the distal sequence element which has many properties in common with transcriptional enhancers contains, in addition to sp1 binding sites, an octamer binding site which mediates activation through interactions with the ubiquitous transcription factor oct-1. in the present study we have attempted to answer the questi ...	1991	1829677
enhanced phagocytosis and intracellular killing of pityrosporum ovale by human neutrophils after exposure to ketoconazole is correlated to changes of the yeast cell surface.	in seborrhoeic dermatitis an inflammatory response occurs secondary to large numbers of pityrosporum yeasts appearing within and beneath the epidermis. to study the interaction between human neutrophils and p. ovale and any immunomodulating effect of antifungal agents, the yeast was exposed to ketoconazole and then incorporated into neutrophil monolayer assays. phagocytosis was complement dependent and reached a maximum after 40 min. ketoconazole at 25, 50 and 100 mg l-1 had no significant effec ...	1991	1833641
cloning of all-1, the locus involved in leukemias with the t(4;11)(q21;q23), t(9;11)(p22;q23), and t(11;19)(q23;p13) chromosome translocations.	chromosomal region 11q23 participates in a number of reciprocal translocations with specific regions of chromosomes 4, 9, 19, and others. these translocations are associated with acute lymphocytic leukemia and acute myelomonocytic, monocytic, and myelogenous leukemia. from a yeast artificial chromosome containing human dna derived from 11q23 we cloned a dna fragment which can be used as a probe to detect rearrangements in leukemic cells from the majority of patients with the t(4;11), t(9;11), an ...	1991	1835902
mitogenic effect of lysosomal hydrolases on bovine tracheal myocytes in culture.	studies were conducted to assess the mitogenic effect of lysosomal hydrolases, enzymes known to have an association with allergen- or ozone-induced airway hyperreactivity, on bovine tracheal myocytes in culture. addition of purified human placental beta-hexosaminidase and partially purified bovine liver beta-glucuronidase resulted in the doubling of cell count after 4 d of incubation in medium m199 with 0.4% fbs. unstimulated cells remained quiescent without a significant increase of cell count. ...	1991	1836469
phosphorylation of replication protein a: a role for cdc2 kinase in g1/s regulation.		1991	1840253