Publications

in vivo measurements of tissue ph of the heart, brain, liver, spleen, and skeletal muscle following therapy of severe metabolic uraemic acidosis with infusion of bases: an experimental study on the limitation of the mellemgaard-astrup equation.	the intracellular tissue ph of the heart, brain, liver, spleen, and skeletal muscle of nephrectomized rats was determined in vivo from the distribution of 14c-labelled dmo (5,5-dimethyl-2-4-oxazolidinedione) following acute variation of arterial plasma ph (phe) by co2 inhalation or sodium bicarbonate administration in the range of phe 6.9-7.7, or following correction of severe metabolic uraemic acidosis with sodium bicarbonate or tris (hydroxymethyl) aminomethane (tham) infusion according to the ...	1987	2820724
atp synthesis by the (na+ + k+)-atpase in the absence of an ionic gradient. effects of organic solvent.	a 200-fold decrease in the pi concentration required for half-maximal phosphorylation of the (na+ + k+)-atpase is observed when 40% (v/v) dimethyl sulfoxide is added to the assay medium. the phosphoenzyme formed in the presence of dimethyl sulfoxide is able to transfer its phosphate to to form atp when concentrations of organic solvent and of na+, and is inhibited by ouabain.	1987	2820794
atp synthesis by the (na+ + k+)-atpase in the absence of an ionic gradient. effects of organic solvent.	a 200-fold decrease in the pi concentration required for half-maximal phosphorylation of the (na+ + k+)-atpase is observed when 40% (v/v) dimethyl sulfoxide is added to the assay medium. the phosphoenzyme formed in the presence of dimethyl sulfoxide is able to transfer its phosphate to to form atp when concentrations of organic solvent and of na+, and is inhibited by ouabain.	1987	2820794
frequency of is1-mediated molecular events in different members of the family enterobacteriaceae.	the numbers of chromosomal copies of the insertion sequence is1 in strains of salmonella typhimurium (0 to 8 copies), shigella sonnei (56 copies), and shigella flexneri (41 copies) isolated in mexico city, mexico, were similar to those reported for these genera isolated in other countries. of the 11 shigella strains studied, all carried several small plasmids; however, in only one of these strains did a small plasmid contain is1, is1 recombination, cointegrate formation mediated by is1 or by the ...	1987	2822657
induction of phr gene expression by irradiation of ultraviolet light in escherichia coli.	to measure the degree of phr gene induction by dna-damaging agents, the promoter region was fused to the coding region of the lacz gene in plasmid pmc1403. the new plasmids were introduced into escherichia coli cells having different repair capabilities. more efficient induction of phr gene expression was detected in a uvra- strain as compared with the wild-type strain. in addition, obvious induction was detected in uvra- cells treated by 4-nitroquinoline 1-oxide and mitomycin c. nalidixic acid, ...	1987	2823069
induction of phr gene expression by irradiation of ultraviolet light in escherichia coli.	to measure the degree of phr gene induction by dna-damaging agents, the promoter region was fused to the coding region of the lacz gene in plasmid pmc1403. the new plasmids were introduced into escherichia coli cells having different repair capabilities. more efficient induction of phr gene expression was detected in a uvra- strain as compared with the wild-type strain. in addition, obvious induction was detected in uvra- cells treated by 4-nitroquinoline 1-oxide and mitomycin c. nalidixic acid, ...	1987	2823069
morphological differentiation of cultured astrocytes is blocked by cadmium or cobalt.	the morphological transformation of astrocytes in primary culture from flat to process bearing is induced by cyclic adenosine monophosphate (camp). calcium currents in cultured astrocytes are also enhanced by camp. this correlation suggests the hypothesis that camp-induced calcium entry causes the morphological differentiation. the experiments reported here show that cadmium or cobalt, which block astrocyte calcium channels, also block the camp-induced morphological differentiation. these result ...	1987	2823972
isolation and characterization of is elements repeated in the bacterial chromosome.	shigella sonnei contains repetitive sequences, including an insertion element is1, which can be isolated as double-stranded dna fragments by dna denaturation and renaturation and by treatment with s1 nuclease. in this paper, we describe a method of cloning the is1 fragments prepared by the s1 nuclease digestion technique into phage m13mp8 rfi dna. several clones contained is1, usually with a few additional bases. we isolated and characterized five other repetitive sequences using this method. on ...	1987	2824781
effects of palindrome size and sequence on genetic stability in the bacteriophage phi x174 genome.	by inserting palindromes of varying length and sequence into a non-essential region of the bacteriophage phi x174 genome we have investigated the effect of palindrome size and sequence on their genetic stability. multimers of increasing size of the ecori linker ccgaattcgg (e), the bamhi linker ccggatccgg (b) or mixtures of both (e, b) were inserted into the pvuii site of a previously constructed bacteriophage strain phi x174 j-f ins6. the largest inserts that could be maintained in the genome wi ...	1987	2824789
dopamine denervation of the nucleus accumbens induces a selective increase in the number of delta-opioid binding sites.	selective lesions of dopamine (da) neurons of the nucleus accumbens were made in rats by local injections of 6-hydroxydopamine (6-ohda). seven days after 6-ohda, the binding affinities and capacities at mu-, delta- and kappa-opioid binding sites were determined in the nucleus accumbens by selective labelling techniques. 6-ohda pretreatment caused a significant increase in the number of delta-opioid binding sites (+30%) while the number of mu-, kappa- and total sites was not modified. the affinit ...	1987	2825926
dopamine denervation of the nucleus accumbens induces a selective increase in the number of delta-opioid binding sites.	selective lesions of dopamine (da) neurons of the nucleus accumbens were made in rats by local injections of 6-hydroxydopamine (6-ohda). seven days after 6-ohda, the binding affinities and capacities at mu-, delta- and kappa-opioid binding sites were determined in the nucleus accumbens by selective labelling techniques. 6-ohda pretreatment caused a significant increase in the number of delta-opioid binding sites (+30%) while the number of mu-, kappa- and total sites was not modified. the affinit ...	1987	2825926
genetic and physical characterization of trimethoprim resistance plasmids from shigella sonnei and shigella flexneri.	analysis of six shigella flexneri and four s. sonnei isolates with trimethoprim (tp) resistance from clinical cases in ontario has shown that, in all isolates, the tp resistance is mediated by gene(s) on conjugative, multiple antibiotic-resistance plasmids. the physical and genetic characterization of these plasmids revealed that there are three different tp resistance plasmids. one group, composed of all six s. flexneri plasmids, consists of plasmids which are about 70 megadaltons (mda) and inh ...	1987	2825949
murine cytomegalovirus-pseudomonas synergistic infections: comparison of virulent and attenuated virus.	the synergistic interactions between pseudomonas aeruginosa and virulent or attenuated murine cytomegalovirus (mcmv) were compared in vivo. virulent mcmv challenge at a dose of 5 x 10(5) pfu/mouse intraperitoneally, followed by intranasal superinfection with 5 x 10(6) cfu/mouse of pseudomonas aeruginosa after 48 h resulted in greater than 80% mortality, apparently owing to a failure of pulmonary clearance mechanisms. single infections, or the use of attenuated mcmv in synergistic infections, did ...	1987	2825950
limb specific connections of the cat magnocellular red nucleus.	afferent and efferent connections of the limb specific divisions of the cat magnocellular red nucleus (rnm) were traced using the bidirectional transport of wheatgerm agglutinin-horseradish peroxidase complex (wga-hrp). injection sites within forelimb or hindlimb rnm regions were identified by microelectrode recording and confirmed by the position of labeled rubrospinal terminals. additional injections into structures that project to, or receive input from, rnm confirmed the somatotopic organiza ...	1987	2826551
metabolic studies of a sustained galactose overload in rat.	in order to obtain an experimental model for studying inherited disorders of galactose metabolism in man, we administered to adult rats a diet supplemented with 50% of galactose during 38 days. the results showed clinical symptoms such as polyuria, polydipsia, growth retardation and bilateral cataract. the main biochemical features were an increase in the galactokinase and uridyltransferase activities in liver, an ubiquitous accumulation in tissues and a considerable urinary elimination of galac ...	1987	2827561
synthesis and biological activity of n-methylated analogues of neurokinin a.	limited conformational constraints have been introduced in the sequence of the c-terminal heptapeptide nka(4-10) by n-methylation of individual peptide bonds and the biological activities of the peptides thus obtained were evaluated on three in vitro preparations sensitive and quite selective for each of the naturally-occurring neurokinins, in order to assess the effect of n-methylation on receptor selectivity. the analogue methylated in position 7, namely [meval7] nka(4-10) shows some selectivi ...	1987	2829053
identification of tonin in brain and exocrine tissues and in the cell-free translation products encoded by the mrna of these tissues.	tissue-specific expression of the esteropeptidase tonin [ec 3.4.99.-] was investigated in rat brain, submandibular gland, pancreas and kidney. specific polyclonal and monoclonal antibodies to purified rat tonin from the submandibular gland have been developed and characterized and have been purified via a tonin-agarose affinity column. immunoreactive tonin was measured by a recently developed tonin direct radioimmunoassay using a rabbit tonin antiserum. resulting tonin levels were found to be 10 ...	1987	2829829
cytomegalovirus infection in burn patients.	we studied the seroepidemiology of cytomegalovirus (cmv) infection in 59 patients with thermal injuries. among the 31 patients who were initially cmv seronegative, seven (22.5%) seroconverted. patients who seroconverted had longer hospital stays (mean, 27 +/- 23.9 days v 14.9 +/- 8.8 days in seronegative patients [p = 0.03]) and they received more blood products (mean, 10.7 +/- 17.9 units v 1.8 +/- 2.8 units [p = 0.02]) than patients who remained seronegative. among 18 patients who were initiall ...	1987	2830289
cerebrovascular effects of isoflurane in man. intracranial pressure and middle cerebral artery flow velocity.	twelve hydrocephalic patients undergoing hydrodynamic studies and shunt procedures during general anaesthesia were subjected to short term observations of the cerebrovascular effects of isoflurane. intraventricular pressure (ivp), arterial pressure (ap); cerebral perfusion pressure (cpp = ap-ivp), end-tidal pco2, cerebral function monitor (cfm) activity and middle cerebral artery (mca) flow velocity were measured. introduction of 1% isoflurane for a 10-min period did not cause significant change ...	1987	3676050
human monoclonal cryoimmunoglobulins. ii. comparative studies on the carbohydrate contents and compositions of cryoglobulins and myeloma proteins.	the carbohydrate contents and compositions of cryoglobulins and myeloma proteins were comparatively investigated by gas-liquid chromatographic and colorimetric analyses. human monoclonal cryoglobulins, jir (igg3 kappa), wat (igg3 lambda), fji (igm kappa), and human myeloma proteins, har (iggl kappa) and kob (iga kappa) were used for the present experiments. the results obtained were that the carbohydrate contents of jir and wat were 2.7 and 4.4% in weight basis; the n-acetyl glucosamine of wat w ...	1987	3685711
distribution of immunoreactive inhibin like material in different regions of human prostate.	distribution of immunoreactive inhibin material (ir-ilm) in different regions of human prostate was studied by using specific radio-immunoassay. mean ir-ilm concentrations in the central, peripheral and periurethral zones were 27.3 +/- 9.3, 2.4 +/- 0.7 and 3.0 +/- 1.5 micrograms/mg protein respectively (p central vs periurethral and peripheral less than 0.05).	1987	3688474
effects of changed lipid composition on responses of liposomes to various odorants: possible mechanism of odor discrimination.	in a previous paper [nomura, t., & kurihara, k. (1987) biochemistry (preceding paper in this issue)], we showed that azolectin liposomes are depolarized by various odorants and there is a good correlation between the responses in the liposomes and the frog or porcine olfactory responses. in this study, we examined effects of changed lipid composition on responses of liposomes to various odorants. the membrane potential changes in response to odorants were monitored with the fluorescent dye 3,3'- ...	1987	3689768
effects of cold stress on brain regional calcium content in rats and mice.	the calcium levels of striatum, cerebellum, cortex, medulla and diencephalon from rat brain were significantly reduced after 30 minutes of cold exposure at 8 degrees c even though body temperatures were no different from pre-cold exposure values. calcium content remained significantly depressed in striatum, cerebellum and diencephalon after 2 hours of cold exposure, at which time body temperatures were significantly depressed. a similar effect of short-term cold stress on calcium content in thes ...	1987	3690357
[clinico-anatomical and immunomicrobiological aspects of infection caused by gram-negative bacteria with lung lesions in children].		1987	3696845
anticholinesterase properties of methamidophos and acephate in insects and mammals.		1987	3814841
use of nude mouse xenografts as preclinical drug screens: in vivo activity of established chemotherapeutic agents against melanoma and ovarian carcinoma xenografts.	to evaluate the utility of nude mouse xenografts as preclinical drug screens, the activity of ten established chemotherapeutic agents was evaluated against seven melanoma and three ovarian carcinoma xenografts. xenografts were established using primary explants from patients who had not received chemotherapy and serially passaged as sc tumors in nude mice. in vivo drug activities for dactinomycin, carmustine, vinblastine, melphalan, amsacrine, cisplatin, bleomycin, mitomycin, doxorubicin, and et ...	1987	3815395
influence of low-dose beta-interferon on natural killer cell activity in breast cancer patients subjected to chemotherapy.	the present study was designed to test whether immunomodulating doses of human beta-interferon would affect the natural cell-mediated cytotoxic function in untreated breast cancer patients or in those subjected to antitumor therapy. analyses were performed on 11 breast cancer patients, 3 at stage 1 and 8 at stage 2, the latter being subjected to cyclophosphamide, methotrexate, 5-fluorouracil (cmf) adjuvant chemotherapy. five patients treated with cmf and 3 patients not subjected to adjuvant chem ...	1987	3815421
influence of low-dose beta-interferon on natural killer cell activity in breast cancer patients subjected to chemotherapy.	the present study was designed to test whether immunomodulating doses of human beta-interferon would affect the natural cell-mediated cytotoxic function in untreated breast cancer patients or in those subjected to antitumor therapy. analyses were performed on 11 breast cancer patients, 3 at stage 1 and 8 at stage 2, the latter being subjected to cyclophosphamide, methotrexate, 5-fluorouracil (cmf) adjuvant chemotherapy. five patients treated with cmf and 3 patients not subjected to adjuvant chem ...	1987	3815421
incidence of shigellosis and multi-drug resistant shigellae: a 10 year study.	between 1976 and 1985 a total of 12,709 faecal samples were cultured. from these samples 760 strains (6.0%) of shigellae were isolated. shigella flexneri was the commonest isolate (55%), followed by s. dysenteriae (29.7%). a total of 15.7% of children under 5 years of age were positive for shigellae. the rate of isolation was lower during the winter months than in the summer months. only 25 strains (3.3%) were sensitive to all five antibiotics tested. a total of 617 (81.2%) were resistant to thr ...	1987	3820357
reaction to injectable collagen: results in animal models and clinical use.	since its commercial release, zyderm collagen implant has been used to treat more than 200,000 subjects in the united states for soft-tissue contour defects and more than 250,000 patients internationally (including the united states). approximately 3 percent of subjects' skin tested with zyderm collagen experience localized hypersensitivity reactions to collagen, whereas approximately 1 percent of treated patients demonstrate symptoms of hypersensitivity at treatment sites. of the latter treatme ...	1987	3823250
cerebrovascular response to intracranial hypertension.	pial arterial and venous calibre changes during intracranial hypertension were studied in 11 cats under barbiturate- and nitrous oxide-anaesthesia by using a closed cranial window technique and multichannel videoangiometry. intracranial pressure was elevated from a normal mean level of 6.4 mm hg by cisternal infusion of mock csf in steps to 20, 30, 40, 50 mm hg and finally to the level of systolic pressure. pial arteries dilated significantly, small ones more than large ones, by 42 +/- 5.6% and ...	1987	3577856
definition of subclasses of adenosine receptors associated with adenylate cyclase: interaction of adenosine analogs with inhibitory a1 receptors and stimulatory a2 receptors.	the structure-activity relationships of 63 adenosine analogs as agonists for the a1 adenosine receptors that mediate inhibition of adenylate cyclase activity in rat fat cells and for the a2 adenosine receptors that mediate stimulation of adenylate cyclase in rat pheochromocytoma pc12 cells and human platelets were determined. the lack of correspondence between the structure-activity relationships of these analogs at the a1 and a2 receptors appear definitive in terms of establishing the existence ...	1987	3580959
physiologic and biochemical effects of immobilization on muscle.	muscle strength rapidly declines during limb immobilization because of a decrease in muscle size and a decrease in tension per unit of muscle cross-sectional area. muscle fatigability also increases rapidly after limb immobilization. muscles within limbs fixed by plaster casts have lower levels of resting glycogen and adenosine triphosphate (atp), a more rapid depletion of muscle glycogen and atp during work, a greater increase in lactate during work, and a decreased capacity to oxidize fatty ac ...	1987	3581565
visual and oculomotor function in optic chiasma-sectioned rabbits.	anatomical and physiological findings indicate that the crossed optic fibres of the rabbit have a crucial role in binocular vision. in order to directly examine the visual functions of the uncrossed fibre system, a technique of sectioning the optic chiasma midsagitally was developed. both normal and chiasma-sectioned rabbits were tested on a variety of visual discrimination tasks as well as such oculomotor control functions as the optokinetic and vestibulo-ocular reflexes. following transection ...	1987	3582536
the connections of the peripeduncular area studied by retrograde and anterograde transport in the rat.	lying just ventral to the medial geniculate nucleus and dorsal to the cerebral peduncle, the peripeduncular area (ppa) seems to be implicated in the expression of several sexual reflexes in rodents. the aim of the present investigation was to establish the organization of the afferent and efferent projections of the rat's ppa in an attempt to provide a neural substrate corresponding to the behavioral observations. based on both retrograde and anterograde transport methods, our results suggest th ...	1987	3584548
development of neural receptors for serotonin in the murine bowel.	during the ontogeny of the enteric nervous system, the varicosities of mature neurons contact dividing neural precursors, which persist in the developing murine gut for several weeks postnatally. this phenomenon has led to the hypothesis that the release of transmitter from mature neurons may influence the subsequent development of uncommitted neuroblasts. in order to test this hypothesis, it is necessary to know the timing of the expression of postsynaptic receptors for enteric neurotransmitter ...	1987	3584550
abnormal daily periodicity of serum thyrotropin (tsh) and evidence for defective tsh suppression in a case of non-neoplastic syndrome of inappropriate tsh secretion.	a non-neoplastic syndrome of inappropriate secretion of tsh (itshs) was diagnosed in a hemithyroidectomized and clinically euthyroid 44-yr-old man, who also exhibited limping (perthes' disease), genu valgum, pes supinatus and lateral nystagmus. computed tomography demonstrated an enlarged sella turcica due to empty sella. baseline serum t3, t4, free t3, free t4 and tsh fluctuated between 179 and 274 ng/dl, 6.0 and 13.2 micrograms/dl, 4.2 and 6.0 pg/ml, 7.6 and 15.3 pg/ml, and 4.3 and 33.0 microu ...	1987	3584859
lyme disease in texas.		1987	3591105
lyme disease in texas.		1987	3591105
estrogen stimulates growth of mammary tumor cells zr-75 without activation of s6 kinase and s6 phosphorylation. difference from epidermal growth factor and alpha-transforming growth-factor-induced proliferation.	growth of the human mammary tumor cell line zr-75-1 is stimulated by epidermal growth factor (egf) and alpha-type transforming growth factor (alpha tgf), as well as by estradiol (e2). the role of activation of s6 kinase and s6 phosphorylation in the egf(alpha tgf)-induced and e2-induced growth was investigated. maximal effects on growth are observed at 10 nm egf or alpha tgf. egf as well as alpha tgf treatment of serum-starved cells leads to rapid activation of s6 kinase; the activity is increas ...	1987	3494604
dexamethasone pre-treatment is antipyretic toward polyinosinic: polycytidylic acid, lipopolysaccharide and interleukin 1/endogenous pyrogen.	the effect of intravenously injected dexamethasone on the febrile response of rabbits to polyinosinic: polycytidylic acid (poly i:c), lipopolysaccharide (lps) and interleukin 1/endogenous pyrogen (il1/e.p.) was studied. dexamethasone (1 mg/kg) attenuated the febrile response to poly i:c (5 micrograms/kg) but only if administered between 0.5 to 2 h before poly i:c. if it was given after poly i:c this resulted in a potentiation of the fever. antagonism of the febrile response to poly i:c by dexame ...	1987	3495738
further improvement of high-speed nmr flow-velocity measurement using a differential phase-encoding technique.	an nmr imaging technique applicable to high-velocity flow imaging is described. by subtraction of two phase images obtained by varying the flow-encoding gradient, it is possible to extract much larger ranges of velocity components. this differential phase-encoding technique is tested by experiments in a phantom as well as a human volunteer.	1987	3574062
treatment of cerebrospinal fluid with formalin from patients infected with human immunodeficiency virus before diagnostic microscopy.		1987	3643930
nonproductive, cell-associated virus exists before the appearance of antiviral antibodies in experimental measles encephalitis.	the ability of measles virus to induce a cell-associated state of infection in the central nervous system (cns) plays an important role in the pathogenesis of subacute sclerosing panencephalitis (sspe), a fatal disease of children and young adults. the mechanism by which such an infection state is established is unknown, but several hypotheses have been proposed. one of these suggests that antiviral antibodies induce the nonproductive infection state by a process termed antigenic modulation. res ...	1987	3811233
circulating forms of human foetal somatomedin.	two forms of somatomedin, which crossreact in a radioreceptor-assay using foetal brain membranes as matrix, have been partially purified from the serum of human foetuses aged 16-28 weeks of gestation. the purification scheme consisted of acid precipitation, sephadex g-50 chromatography, affinity chromatography and cation exchange fast protein liquid chromatography. the two peaks of activity had apparent molecular weights of approximately 7000 and different isoelectric points. the elution positio ...	1987	3811771
dose-titration of fenbendazole in the treatment of poultry nematodiasis.	broiler chickens were fed ad libitum diets containing fenbendazole at various concentrations and durations for the removal of adult ascaridia galli, heterakis gallinarum, and capillaria obsignata. all regimens were 100% effective against a. galli. fenbendazole at doses of 80 ppm for 3 days and 48 or 30 ppm for 5 days was 100% effective against h. gallinarum. c. obsignata was not removed completely by any regimen, but greater than or equal to 95% effectiveness was seen in birds given fenbendazole ...	1986	3814008
furosemide lowers intracranial pressure by inhibiting csf production.	furosemide administration effectively lowers intracranial pressure in newborn preterm and term rabbit pups. this effect may be due to the diuretic action of the drug, its ability to inhibit cerebrospinal fluid production or to a combination of both. to test these possibilities newborn rabbits were either injected with furosemide and left unmolested for 6 hours, or anaesthetized and subjected to ventriculocisternal perfusions. during the 6 hour postnatal period the decrease in body weight was 8 t ...	1986	3811613
determination of mevinolin and mevinolinic acid in plasma and bile by reversed-phase high-performance liquid chromatography.		1986	3644824
[evaluation of the usefulness of routine serologic tests in the diagnosis of human brucellosis in the current epidemiologic situation].		1986	3575762
angiostrongylus cantonensis infection in ampullarius canaliculatus (lamarck) in kyushu, japan.	ampullarius canaliculatus is a large fresh-water snail of which the original habitat is said to be argentina. recently, the snail which grew wild has propagated in various parts of japan, and is causing damage to aquatic plants by feeding on them. the present study was performed to find out whether the snail can be a suitable intermediate host for angiostrongylus cantonensis. natural infection with a. cantonensis was confirmed in the snails collected from 5 different places in 4 hamlets in okina ...	1986	3576290
local treatment with epurox (superoxide dismutase and catalase of human origin) in the chronic inflammatory and degenerative rheumatism.		1986	3496868
[structural studies of panax ginseng c. a. mey pectin].		1986	3591331
ostertagia ostertagi: excretory secretory chemotactic substance from infective larvae as cause of eosinophil locomotion.	the infective (third stage) larva of ostertagia ostertagi produced an excretory secretory substance that is chemotactic for bovine eosinophils. the eosinophil chemotactic substance was present in supernatants of larval cultures within 6 hr of incubation in eagle's minimum essential medium containing 25 mm n-2-hydroxyethylpiperazine-n'-2-ethanesulfonic acid. the substance responsible for eosinophil chemotaxis has activity in small amounts, a molecular weight (mw) greater than 2000, and is heat la ...	1986	3943588
ostertagia ostertagi: excretory secretory chemotactic substance from infective larvae as cause of eosinophil locomotion.	the infective (third stage) larva of ostertagia ostertagi produced an excretory secretory substance that is chemotactic for bovine eosinophils. the eosinophil chemotactic substance was present in supernatants of larval cultures within 6 hr of incubation in eagle's minimum essential medium containing 25 mm n-2-hydroxyethylpiperazine-n'-2-ethanesulfonic acid. the substance responsible for eosinophil chemotaxis has activity in small amounts, a molecular weight (mw) greater than 2000, and is heat la ...	1986	3943588
trichinella spiralis: newborn larval migration route in rats reexamined.	the route by which trichinella spiralis newborn larvae migrate from the small intestine to striated muscle was studied in inbred ao and random-bred sprague-dawley rats. newborn larvae were quantitatively recovered from the thoracic duct lymph, peritoneal cavity, and hepatic portal vein blood during the course of a primary infection with 4000 muscle larvae. the total recovery of newborn larvae assessed in this manner was compared with the number of muscle larvae in control rats receiving the same ...	1986	3943594
on dying more than one death.	death (with a capital d) can best be understood as a series of distinct but related deaths. for example, a pregnant woman was found to be brain-dead but her vital functions were artificially sustained for nine weeks until her fetus could be delivered, after which the machines were removed and she died a second, conceptually distinct death. this procedure is probably justifiable, but any legislation or policy regarding such cases should be flexible and should require consent.	1986	3957650
[secondary cutaneous mycoses caused by alternaria species].	analysis of 2 of our own cases of cutaneous alternariosis (ca) and 31 reported cases showed this mycosis to be nosologically heterogeneous. ca may present as an endogenous, multilocular form or as an exogenous, usually localized form. in the endogenous form of ca (11 cases), infection most probably occurs via the lung by inhalation. the highly dermatotropic organisms spread hematogenically to the skin where they cause the typical verruciform or granulomatous lesions. in the exogenous form (8 cas ...	1986	3957668
retinal constraints on orientation specificity in cat visual cortex.	most retinal ganglion cells (levick and thibos, 1982) and cortical cells (leventhal, 1983; leventhal et al., 1984) subserving peripheral vision respond best to stimuli that are oriented radially, i.e., like the spokes of a wheel with the area centralis at the hub. we have extended this work by comparing directly the distributions of orientations represented in topographically corresponding regions of retina and visual cortex. both central and peripheral regions were studied. the relations betwee ...	1986	3958796
[the systematic evaluation of medical procedures. apropos of the effectiveness of parallel medicine].		1986	3961360
response of dog tracheal stretch receptors to high frequency oscillations.	in eight dogs, anaesthetized and spontaneously breathing, we recorded from thin vagal filaments action potentials from eight slowly adapting receptors of the extrathoracic trachea. the extrathoracic trachea was cut longitudinally and the cut-ends of the cartilage corresponding to the site of the receptor in the trachealis muscle were stretched sinusoidally at frequencies from 0.5 to 39.5 hz. the number of spikes per cycle decreased with increasing frequency. at the highest frequency (40 hz), str ...	1986	3697539
spinal cord serotonin release and raised blood pressure after brainstem kainic acid injection.	the recently developed technique of in vivo dialysis has permitted us to make direct measurements of serotonin release in a specific region of the spinal cord and to relate this to changes in blood pressure elicited by chemical stimulation of the brainstem. in the present experiments we have shown that chemical stimulation of bulbospinal neurons in the region of the b3 cell group in the ventromedial medulla, causes an increase in the release of serotonin in the thoracic spinal cord and that this ...	1986	3697690
evidence for the presence of a novel biosynthetic pathway for norspermidine in vibrio.	enzymatic studies of the cell extracts of vibrio alginolyticus and v. parahaemolyticus provided evidence that there exists a novel biosynthetic pathway for norspermidine (nh2(ch2)3nh(ch2)3nh2), a major polyamine species. in this pathway, the schiff base formed between aspartic beta-semialdehyde and 1,3-diaminopropane is first reduced by a nadph-dependent enzyme to yield "carboxynorspermidine" (nh2(ch2)3nh(ch2)2ch(nh2)cooh), which is in turn decarboxylated by a pyridoxal phosphate dependent enzym ...	1986	3697846
rate of incorporation of [3h]thymidine in various tissues of the mouse. a basis for the evaluation of genotoxic effects of chemicals.	[3h]thymidine has been extensively used as a selective precursor to dna in studies on the kinetics of cell proliferation. we have become interested in measuring early inhibition of the dna synthesis in various organs of intact animals for detecting genotoxic properties of chemicals. such experiments should, for convenience and to achieve a large capacity, be performed in the simplest way possible. the present paper deals with some practical aspects on the use of [3h]thymidine in vivo. [6-3h]thym ...	1986	3698076
[cytogenetic activity of metabolites of pesticides representing different classes of chemical compounds].	the cytogenetic activity of some substances formed in agricultural plants during metabolism of pesticides of four classes of chemical compounds was studied in the culture of human peripheric blood lymphocytes. metabolites were shown either to have mutagenic properties similar to those of the initial compounds (ziramtetramethylthiourea, both being mutagens; captan-phthalimide, both possessing no cytogenetic activity) or to be considerably transformed in comparison with them as a result of deactiv ...	1986	3705167
somatotopic organization of motoneurons innervating the pronators, carpal and digital flexors and forepaw muscles in the dog: a retrograde horseradish peroxidase study.	central distribution of motor neurons innervating the individual forearm and forepaw muscle through the median and ulnar nerves was studied in the dog using the retrograde horseradish peroxidase (hrp) method. all hrp-labeled cells were seen ipsilaterally in restricted parts within the dorsolateral (dl) and retrodorsolateral nucleus (rdl) of the ventral horn at levels from the cranial tip of c7 to the cranial third of t2. the results showed that motoneurons situated more cranially supply the more ...	1986	3708348
increased collateralization of the cerebellothalamic pathway following neonatal hemicerebellectomy.	the postlesional reorganization of the cerebellothalamic cells was here studied using a multiple retrograde tracing technique. to this purpose, the cerebellothalamic cell population was investigated in 3 groups of adult rats: (a) cases hemicerebellectomized at birth; (b) cases hemicerebellectomized in adulthood; and (c) control unlesioned ones. in all of the groups the fluorescent tracers diamidino yellow and fast blue were injected bilaterally in the thalamus and the retrograde labeling obtaine ...	1986	3708349
the effect of amiodarone on thyroxine kinetics.	amiodarone was given by daily intraperitoneal injection (10 mg/kg body weight) to a group of 11 new zealand white rabbits. over 6 weeks there were significant increases (p less than 0.01) in plasma total t4 concentration (43 +/- 8 to 60 +/- 13 nmol/l; mean +/- 1 sd) and rt3 concentration (0.12 +/- 0.06 to 0.31 +/- 0.16 nmol/l) and a significant fall in total plasma t3 (2.3 +/- 0.3 to 1.7 +/- 0.2 nmol/l). the plasma clearance of t4 in rabbits treated with amiodarone for 6 weeks was significantly ...	1986	3708866
monoclonal antibodies to human eosinophil plasma membrane antigens enhance the secretion of eosinophil cationic protein.	this study was done to examine the nature of the membrane constituents involved in the secretion of eosinophil cationic protein (ecp) from human blood eosinophils. three mouse monoclonal antibodies were used, which showed greater binding to membrane antigens on activated, and light density eosinophils from patients with an eosinophilia, than on nonactivated or normal density eosinophils. all three antibodies (eon4, eon5 & eon6) stimulated normal density human eosinophils to secrete ecp, either a ...	1986	3708911
[effect of aevite and riboflavin on the electrolyte balance in the wall of the hepatic blood vessels in experimental toxic hepatitis].	the effects of aevite and riboflavin on electrolyte balance in the vessel wall were studied in toxic hepatitis in the cat. significant changes in the contents of na+, k+, ca2+ and mg2+ ions in the hepatic blood vessels were found in all periods of observation. the treatment of toxic hepatitis with aevite and riboflavin results in normalization of electrolyte balance of the vessel wall.	1986	3709781
[hemodynamic correlates of immobilization stress in the rat].	changes of the systemic and regional hemodynamics were studied in alert wistar rats during their immobilization. cardiac output and regional blood flows were measured with microsphere technique. the stress evoked 12% blood pressure increase, 30% cardiac output increase and 10% total peripheral vascular resistance decrease. blood flow was increased in skeletal muscles, in the heart and adrenal glands, whereas in the kidney and small intestine blood flow decreased.	1986	3709865
low-level lead exposure affects latent learning in the rat.	neonatal rats were administered intragastrically either lead acetate (50 mg pb/kg-bw) or an equal molar solution of sodium acetate at days 6, 9, 12, 15, and 18 postpartum. at 33 days of age, each animal began a training sequence to develop maze running skills. animals within each treatment group, i.e., lead exposed (pb), control vehicle (cv), and control nonhandled (cnh) were assigned randomly to either latent learning or open-field testing groups. the former individually explored a symmetrical ...	1986	3713959
reversible electroencephalogram changes associated with administration of baclofen in a quadriplegic patient: case report.	we present the case of a spastic quadriplegic who developed mental symptoms which resolved when his baclofen was discontinued. of interest was the presence of eeg abnormalities similar to those described in cats receiving this drug. these abnormalities, previously unreported in humans, resolved upon discontinuing baclofen therapy.	1986	3714292
leptospira hardjo agalactia in sheep.		1986	3716127
multifocal eosinophilic granuloma. response of a patient to etoposide.	a 60-year-old man with a history of diabetes insipidus presented with a left groin mass, leg edema, and retroperitoneal adenopathy. biopsy results of the involved lymph nodes were typical of eosinophilic granuloma. the patient was treated with etoposide and prednisone and had a complete regression of his lymphadenopathy and edema. this response suggests that etoposide may be a useful agent in the management of histiocytosis x.	1986	3719552
tumor metastasis-associated heparanase (heparan sulfate endoglycosidase) activity in human melanoma cells.	we discovered previously a tumor metastasis-associated heparan sulfate (hs)-degrading endoglycosidase in mouse melanoma cells that is a unique endo-beta-glucuronidase (heparanase) capable of specifically cleaving hs at intrachain sites (nakajima et al., j. biol. chem., 259 (1984) 2283. using unmodified- and chemically modified-hs and heparin substrates we demonstrate that human hs939 and mouse b16 melanoma cells possess a hs-degrading endoglycosidase of similar specificity. melanoma heparanase s ...	1986	3719568
detection of extravasation of antineoplastic drugs by microwave radiometry.	microwave radiometry may be used as a non-invasive technique for subcutaneous thermal sensing. this technique was capable of demonstrating extravasation of small volumes of fluids, including the antineoplastic agent adriamycin, when these fluids were administered at room temperature in dogs. the rate of temperature drop upon extravasation was proportional to the flow rate. it is feasible that microwave radiometry may be useful as an alarm system to reduce serious complications which often accomp ...	1986	3719569
serum immune response to shigella protein antigens in rhesus monkeys and humans infected with shigella spp.	the serum antibody response to proteins encoded by the virulence-associated plasmid of shigella flexneri was determined in monkeys challenged with virulent s. flexneri serotype 2a. with water-extractable antigen in an enzyme-linked immunosorbent assay, a significant increase in antibody titer against proteins from a plasmid-carrying, virulent strain of s. flexneri serotype 5 could be demonstrated in convalescent sera. there were minimal antibody titers against proteins from an avirulent (plasmid ...	1986	3721580
effect of thyroid hormones on plasma protein concentrations in man.	plasma concentrations of proteins secreted by the liver (prealbumin, haptoglobin, transferrin, ceruloplasmin, alpha 1-antitrypsin, antithrombin iii, and t4-binding globulin) and proteins mainly derived from endothelium [fibronectin, angiotensin-converting enzyme (ace), and factor viii-related antigen (f viii r:ag)] were measured in 27 hyperthyroid and 30 normal women. significantly increased plasma concentrations (p less than 0.01) of endothelium-associated proteins, including fibronectin, ace, ...	1986	3722331
salmonella hepatitis: detection of salmonella antigens in the liver of patients with typhoid fever.		1986	3722872
renal function in the fetal lamb: a chronic model to study physiological effects of ureteral ligation and deligation.	between 53 and 98 days of gestation 11 fetal lambs were subjected to unilateral ureteral ligation. in 8 fetuses re-exploration was performed 35 to 41 days after ligation. chronic catheterization of the obstructed kidney, bladder and fetal vascular system was used for analysis of physiological data. average creatinine clearance was 1.35 +/- 0.067 ml. per minute in the control kidney and 0.226 +/- 0.057 ml. per minute in the obstructed kidney after diversion. free water clearance, osmolar clearanc ...	1986	3723669
[clostridium difficile and its significance as an intestinal pathogenic bacterium].		1986	3725396
[associated vaccination of pigs against swine fever, erysipelas and aujeszky's disease].	tested was the simultaneous vaccination of pigs against swine fever, swine erysipelas, and aujeszky's disease. parallel to this experiment a second group of test pigs were treated with each of the three vaccines at twenty-day intervals. the effect of vaccination was assessed through clinical observations with the test groups and by challenging part of the animals with virulent strains of the swine fever and erysipelas agents. it was found that the successive application of the three vaccines at ...	1986	3727391
oxidative injury to the vascular endothelium.	in recent years, increasing clinical and experimental data have provided compelling evidence that neutrophil-derived oxygen radicals and their metabolites are important mediators of vascular endothelial injury. in this review, attention is directed toward in vitro, ex vivo, and in vivo experimental studies contributing to current understanding of mechanisms of oxyradical mediated endothelial damage. although these studies may have broad significance, they appear to have particular relevance to t ...	1986	3728559
low-molecular-weight proteinase inhibitor of human epidermis inhibiting papain and trypsin activity.	a low-molecular-weight proteinase inhibitor was isolated from human epidermal extracts by means of ultrafiltration, gel filtration and ion-exchange chromatography. on polyacrylamide gel electrophoresis (page), the isolated fraction exhibited a single band of activity at ph 9.4, and inhibitory activity against papain and trypsin was detected in this band. using sodium-dodecyl-sulphate page, the molecular mass of the inhibitor was estimated to be 2,200 daltons.	1986	3729540
[induction of cytochrome p-450 and b5 in hepatocyte subpopulations with phenobarbital].	hepatocytes isolated from intact and phenobarbital-treated rats were separated by ficoll discontinuous density gradient into five subpopulations. about 85% of the total cell number sedimented within the range of 1.044 to 1.126 g x cm-3. in intact rats, heavy hepatocytes contained about twice as much cytochrome p-450 and about 3 times as much cytochrome b5 as light cell population. phenobarbital induced cytochrome p-450 in all subpopulations, with a more pronounced increase observed in light hepa ...	1986	3730555
[induction of cytochrome p-450 and b5 in hepatocyte subpopulations with phenobarbital].	hepatocytes isolated from intact and phenobarbital-treated rats were separated by ficoll discontinuous density gradient into five subpopulations. about 85% of the total cell number sedimented within the range of 1.044 to 1.126 g x cm-3. in intact rats, heavy hepatocytes contained about twice as much cytochrome p-450 and about 3 times as much cytochrome b5 as light cell population. phenobarbital induced cytochrome p-450 in all subpopulations, with a more pronounced increase observed in light hepa ...	1986	3730555
vacant postsynaptic densities on supraoptic dendrites of adult rats diminish in number with chronic stimuli.	in earlier ultrastructural studies of the supraoptic nucleus in adult rats we noted "free" and incompletely covered postsynaptic densities (collectively referred to here as vacant postsynaptic densities) on dendritic shafts. free postsynaptic densities have been reported in other parts of the central nervous system of normal rodents. we investigated the possibility that physiological activation of the supraoptic cells, which produces changes in many aspects of their morphology, would alter the i ...	1986	3731249
structure and x-ray amino acid sequence of a bacteriochlorophyll a protein from prosthecochloris aestuarii refined at 1.9 a resolution.	the structure of the water-soluble bacteriochlorophyll a protein (bchl protein) from the green photosynthetic bacterium prosthecochloris aestuarii has been refined at 1.9 a resolution to a crystallographic residual of 18.9%. the refinement was carried out without knowledge of the amino acid sequence and has led to an "x-ray sequence". the structure consists of seven bchl molecules enclosed within a protein "bag" and the refinement supports the general conformation of the molecule described previ ...	1986	3735428
age related heterogeneity of lipopigments in human sympathetic ganglia.	the histochemical, autofluorescence and morphological characteristics of lipopigments accumulating with age in the human sympathetic neurons were studied. the spectral characteristics of the lipopigment autofluorescence change with age suggesting the accumulation of additional components in the residual bodies. this component is electron microscopically highly osmiophilic and stains also with silver staining. these changes may indicate the gradual melanization of the lipopigments due to auto-oxi ...	1986	3736128
[a method for registration of stomach movement of rabbits in chronic experiments].		1986	3738532
fragile papilloma (papillary adenoma) of the gallbladder. a cause of recurrent biliary colic.	this case study of a young man with a papillary adenoma of the gallbladder documents the potential for benign tumors of the gallbladder to present with symptoms of biliary colic. the case illustrates the mechanism by which free-floating, detached, tumor particles cause intermittent obstruction of the cystic duct, and it presents the attendant symptoms.	1986	3743976
comparison of the surface morphology of adult schistosoma japonicum (chinese, philippine and indonesian strains) by scanning electron microscopy.		1986	3744663
[euphorbia and high chapparall--hazardous pot plants in fashion].		1986	3747661
[campylobacter pyloridis--a cause of gastritis/ulcer?].		1986	3747663
pressure evaluation of the antireflux ability of the rat ureterovesical junction.	the bladder pressure necessary to cause vesicoureteral reflux (vur) was measured in 16 female rats. under general anesthesia, the ureters were exposed via an abdominal incision and a pressure catheter was placed near the ureterovesical junction. values of bladder distension and bladder pressure increase to cause vur were obtained by injecting isotonic saline in one ureter until vur in the opposite ureter was detected as a sudden pressure increase. after 5 min the same procedure was done on the c ...	1986	3750580
vitamin e deficiency in rabbits receiving a high pufa diet with and without a non-absorbable antioxidant. 1. incorporation of [1-14c]glycine into skeletal muscle proteins.	previous studies of metabolic changes in vitamin e-deficient animals were often difficult to interpret because the vitamin e-free, high pufa diets used in such studies may have contained high levels of peroxides. presumed effects of vitamin e deficiency could have been effects of peroxide consumption. addition of the non-absorbable polymeric antioxidant anoxomer to a semisynthetic vitamin e-free diet containing cod liver oil was found to suppress peroxide formation. nevertheless, this diet produ ...	1986	3751156
[reactogenic properties and immunogenicity of measles and mumps vaccines when used by different procedures].	in the controlled epidemiological trial the reactogenic and immunogenic properties of live measles vaccine (lmv) and live parotitis vaccine (lpv) were evaluated in identical groups of children immunized in accordance with different schedules: only lmv or lpv, the injection of these preparations mixed in one syringe or with the use of separate syringes, or at an interval of 3 months. the highest number of febrile reactions, rash and catarrhal symptoms was registered among children immunized with ...	1986	3751401
[reactogenic properties and immunogenicity of measles and mumps vaccines when used by different procedures].	in the controlled epidemiological trial the reactogenic and immunogenic properties of live measles vaccine (lmv) and live parotitis vaccine (lpv) were evaluated in identical groups of children immunized in accordance with different schedules: only lmv or lpv, the injection of these preparations mixed in one syringe or with the use of separate syringes, or at an interval of 3 months. the highest number of febrile reactions, rash and catarrhal symptoms was registered among children immunized with ...	1986	3751401
1,25-dihydroxyvitamin d3 treatment results in increased choline acetyltransferase activity in specific brain nuclei.	to better understand the role of 1,25-dihydroxyvitamin d3 [1,25-(oh)2d3] in brain function, the level of calcium-binding protein (cabp) and the activities of choline acetyltransferase (cat) and monoamine oxidase were measured in discrete brain nuclei of vitamin d-deficient and -replete male rats. the nuclei sampled were those in which receptors for 1,25-(oh)2d3 and/or vitamin d-dependent cabp have been localized. significant elevations in cat activity were found in the arcuatemedian eminence and ...	1986	3753932
the effect of arylazido aminopropionyl atp (anapp3) on inhibition of pelvic nerve evoked contractions of the cat urinary bladder.	previously reported work has provided evidence that arylazido aminopropionyl atp (anapp3), a p2-receptor antagonist, blocks the effects of atp and other purine analogs, in the urinary bladder of the cat. this antagonism appeared limited to those effects mediated by p2-receptors, such as contraction of the urinary bladder. however, it was noted that anapp3 did alter the effects of adenosine, mediated through p1-receptors, in some preliminary experiments. a series of experiments was undertaken to ...	1986	3753940