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multilocus sequence analysis and rpob sequencing of mycobacterium abscessus (sensu lato) strains.mycobacterium abscessus, mycobacterium bolletii, and mycobacterium massiliense (mycobacterium abscessus sensu lato) are closely related species that currently are identified by the sequencing of the rpob gene. however, recent studies show that rpob sequencing alone is insufficient to discriminate between these species, and some authors have questioned their current taxonomic classification. we studied here a large collection of m. abscessus (sensu lato) strains by partial rpob sequencing (752 bp ...201021106786
assessment of clarithromycin susceptibility in strains belonging to the mycobacterium abscessus group by erm(41) and rrl sequencing.clarithromycin was the drug of choice for mycobacterium abscessus infections until inducible resistance due to erm(41) was described. because m. abscessus was split into m. abscessus sensu stricto, mycobacterium massiliense, and mycobacterium bolletii, we looked for erm(41) in the three species and determined their clarithromycin susceptibility levels. ninety strains were included: 87 clinical strains from cystic fibrosis patients (61%) and others (39%), representing 43 m. abscessus, 30 m. massi ...201021135185
chronic mycobacterium abscessus infection and lung function decline in cystic fibrosis.although nontuberculous mycobacteria (ntm) are recognized pathogens in cystic fibrosis (cf), associations with clinical outcomes remain unclear.201020071249
mycobacterium abscessus infection in a cystic fibrosis patient: a difficult to treat infection. 201020074421
laboratory maintenance of mycobacterium abscessus.mycobacterium abscessus, is an emerging, rapidly growing pathogen, with the ability to cause chronic lung disease. this unit covers background information and laboratory maintenance procedures for this bacterium, including growth in liquid and on solid medium. it also contains recommendations concerning long-term strain storage. m. abscessus is a biosafety level 2 organism, and the required safety measures are also discussed.201020812213
genetic manipulation of mycobacterium abscessus.this unit covers genetic manipulation protocols for m. abscessus, including nucleic acid extraction (plasmid dna, genomic dna, and rna), transformation, and a recombineering mutagenesis method. m. abscessus is a biosafety level 2 (bsl-2) bacterium, and working considerations are also discussed.201020812214
efficient differentiation of mycobacterium abscessus complex isolates to the species level by a novel pcr-based variable-number tandem-repeat assay.a novel duplex pcr method based on variable-number tandem-repeat targets to discriminate among mycobacterium abscessus complex isolates was developed and evaluated in 85 clinical isolates. the assay accuracy was confirmed by a multiple-target sequence analysis. the duplex pcr assay is a one-step, reliable, and accurate assay for discriminating m. abscessus species.201021177894
mycobacterium abscessus infection complicating a professional tattoo.a healthy 23-year-old man presented with a tender papular eruption confined to the grey pigment of a recently acquired professional tattoo. atypical mycobacterial infection was suspected and culture of a tissue specimen grew mycobacterium abscessus. he was successfully treated with minocycline and subsequently, clarithromycin. we present a brief review of m. abscessus infection, with a particular focus on its role in nosocomial infections and in the post-tattoo setting.201021198529
a case of mycobacterium abscessus skin infection caused by multiple acupunctures. 201019758379
familial clustering of pulmonary nontuberculous mycobacterial disease.nontuberculous mycobacteria (ntm) are environmental organisms associated with pulmonary disease without person-to-person transmission. although genetic causes of disseminated ntm infection are well characterized, genetic causes for most human susceptibility to pulmonary ntm infection have not been determined.201019858235
onco-reconstructive techniques in the treatment of tuberculosis of the breast.a 54-year-old woman presented with mastitis, which did not respond to conventional oral antibiotic therapy. tissue biopsy led to the diagnosis of tuberculosis of the breast. the underlying causative organism was found to be mycobacterium abscessus, recognised as being a particularly pathogenic strain of tuberculosis.(1) initial treatment involved surgical debridement and antibiotic therapy. following this, onco-reconstructive techniques were used to remove scarred tissue from the affected side a ...201019892613
host immune response to rapidly growing mycobacteria, an emerging cause of chronic lung disease.rapidly growing mycobacteria (rgm) are environmental organisms classified under the broader category of nontuberculous mycobacteria. the most common rgm to cause human diseases are mycobacterium abscessus, mycobacterium chelonae, mycobacterium fortuitum, and mycobacterium massiliense. infections due to the rgm are an emerging health problem in the united states. chronic pulmonary disease and skin/soft-tissue infections are the two most common disorders due to these organisms. clinical outcomes i ...201020081053
nontuberculous mycobacterial lung disease.there is increasing awareness that nontuberculous mycobacteria (ntm) lung diseases are becoming more prevalent. nontuberculous mycobacterial diseases could legitimately be seen as an emerging public-health threat.201020087178
[follow-up on an outbreak in venezuela of soft-tissue infection due to mycobacterium abscessus associated with mesotherapy].skin and soft tissue infections caused by nontuberculous mycobacteria (nmt) are reported to be associated with injections, liposuction, plastic surgery, and acupuncture. herein, we describe an outbreak of soft tissue infection due to nmt following mesotherapy, a cosmetic procedure involving injection of poorly defined mixtures alleged to reduce local adiposity.201020106554
perigastrostomy infection caused by mycobacterium abscessus in an immunocompetent patient. 201020107743
genitourinary infections caused by nontuberculous mycobacteria at a university hospital in taiwan, 1996-2008.genitourinary infections caused by nontuberculous mycobacteria (ntm) are rarely reported. the medical records of all patients with genitourinary ntm infections treated at national taiwan university hospital from 1996-2008 were retrospectively reviewed. fifteen patients were identified, of whom 10 (67%) were male. more than two-thirds of patients had underlying conditions, the most common of which was chronic renal disease. only one patient had aids. acid-fast smears of urine were negative in all ...201020132253
high efficacy of clofazimine and its synergistic effect with amikacin against rapidly growing mycobacteria.the aim of this study was to determine whether clofazimine, dapsone and cycloserine may be suitable antimicrobial agents for the treatment of infections due to rapidly growing mycobacteria (rgm). the antimicrobial activity of the three drugs against 117 mycobacterium abscessus isolates, 48 mycobacterium fortuitum isolates and 20 mycobacterium chelonae isolates was evaluated based on their broth microdilution minimal inhibitory concentrations (mics) against the isolates. clofazimine was highly ef ...201020138481
[clinical analysis of mycobacterium abscessus pulmonary infection in our hospital].we analyzed the clinical characteristics of pulmonary infection due to mycobacterium abscessus.201020143670
lung disease caused by nontuberculous mycobacteria.although the incidence of tuberculosis has reduced in developed countries, there is a growing interest in nontuberculous mycobacteria (ntm) as a cause of lung disease. however, ntm are a heterogeneous group and most of the data come from only three species: mycobacterium avium complex, mycobacterium kansasii and mycobacterium abscessus. still, information about these three species is confusing because it is based mainly on retrospective studies and series of clinical cases performed in developed ...201020154623
[cutaneous and soft skin infections due to non-tuberculous mycobacteria].the frequency of isolation as well as the number of species of non-tuberculous mycobacteria (ntm) has increased in the last years. nearly every pathogenic species of ntm may cause skin and soft tissue infections, but rapidly growing mycobacteria (mycobacterium fortuitum, mycobacterium chelonae and mycobacterium abscessus), mycobacterium marinum and mycobacterium ulcerans are the most commonly involved. many of these cutaneous mycobacteriosis, such as rapidly growing mycobacteria, m. marinum, myc ...201020172423
a case of chronic otitis media caused by mycobacterium abscessus.although it appears very uncommon in adult com, mycobacterium abscessus should be considered as a possible cause of a chronically draining ear. multi-antibiotic chemotherapy including high-dose clarithromycin can effectively treat adult com cased by m. abscessus. the first case report of adult chronic otitis media (com) caused by m. abscessus is described here. a 61-year-old woman presented persistent otorrhea for 2 months, despite treatment with standard antimicrobial drugs. physical examinatio ...201020206453
[three cases of panniculitis due to mycobacterium abscessus after mesotherapy]. 201020223169
mycobacterium abscessus endophthalmitis after descemet's stripping with automated endothelial keratoplasty.to report the first case of endophthalmitis after descemet's stripping with automated endothelial keratoplasty caused by mycobacterium abscessus.201020335806
pulmonary mycobacterium abscessus in an aids patient.mycobacterium abscessus is an unusual pathogen in hiv/aids patients. we report a case of m abscessus with review of the literature, which revealed 1 confirmed case in an hiv/aids patient. preliminary diagnosis was presumed to be pulmonary tuberculosis secondary to positive acid-fast bacilli in sputum stain. after initial treatment failure, sputum stains were repeated, and the species was identified; therefore the antibiotic regimen was tailored for m abscessus. early recognition and treatment of ...201020375687
lung transplantation in patients with cystic fibrosis and mycobacterium abscessus infection.mycobacterium abscessus lung disease is difficult to treat and has been considered a strong relative contraindication to lung transplantation. we performed double lung transplantation in three cystic fibrosis patients with ongoing, and a fourth with recent treatment for mycobacterium abscessus lung infection. despite prolonged antibiotic courses and adjustment of immunosuppressive therapy the first three patients developed skin infection and abscesses. at follow-up after 1, 3, 5 and 7years respe ...201020400381
oral moxifloxacin and topical amikacin for mycobacterium abscessus keratitis after laser in situ keratomileusis.we report a case of mycobacterium abscessus keratitis after elective laser in situ keratomileusis in a 19-year-old woman. the infection started 3 weeks after uneventful surgery, and the patient received multiple antimicrobial treatments without response. a corneal scrape isolate showed an acid-fast bacterium that was subsequently confirmed by culture to be m abscessus. prompt treatment of oral moxifloxacin 400 mg twice daily and hourly topical amikacin 2.5% was started. recovery occurred within ...201020457379
utility of high-performance liquid chromatography analysis of mycolic acids and partial 16s rrna gene sequencing for routine identification of mycobacterium spp. in a national reference laboratory.high-performance liquid chromatography analysis of mycolic acids and partial gene sequencing for the first 500-bp 5' end of the 16s rrna gene were used singularly and in combination to evaluate the final identification of species. examination of 200 cultures revealed 100 strains of slowly growing mycobacteria (sgm), 91 strains of rapidly growing mycobacteria (rgm), and 9 strains of other genera. sgm were discriminated in complexes with both methods for 56 strains, composed primarily of the mycob ...201020466195
computational prediction of properties and analysis of molecular phylogenetics of polyketide synthases in three species of actinomycetes.polyketides are secondary metabolites of microorganisms synthesized by serialized reactions of a set of enzymes called polyketide synthases (pks). as many infectious microorganisms are acquiring tolerance to antibiotics, the need for novel medicines is increasing. recently, various methods are being used for drug discovery, including gene manipulation for biosynthesis of antibiotics such as polyketides. due to their importance as drugs, the volume of data on polyketides is rapidly increasing. in ...201020470251
aerosolized amikacin and oral clarithromycin to eradicate mycobacterium abscessus in a patient with cystic fibrosis: an 8-year follow-up. 201020503290
epidemic of surgical-site infections by a single clone of rapidly growing mycobacteria in brazil.our aim is to investigate if the clusters of postsurgical mycobacterial infections, reported between 2004 and 2008 in seven geographically distant states in brazil, were caused by a single mycobacterial strain.201020521940
mycobacterium massiliense is differentiated from mycobacterium abscessus and mycobacterium bolletii by erythromycin ribosome methyltransferase gene (erm) and clarithromycin susceptibility patterns.erythromycin ribosome methyltransferase gene (erm) sequences of mycobacterium massiliense and mycobacterium bolletii isolates were newly investigated. forty nine strains of m. massiliense that were analyzed in the present study had a deleted erm(41). due to a frame-shift mutation, large deletion, and truncated c-terminal region, the erm(41) of m. massiliense had only 81 amino acids encoded by 246 nucleotides. corresponding to these findings, most of the m. massiliense isolates (89.8%) were marke ...201020536733
an outbreak of skin and soft tissue infection caused by mycobacterium abscessus following acupuncture.although outbreaks of mycobacterium abscessus infection have been reported, none of these reports has identified the potential sources of infection and modes of transmission. in april 2008, we identified and investigated an outbreak of m. abscessus skin and soft tissue infections following acupuncture among the patients who visited an oriental medical clinic. active surveillance of patients who had visited the clinic was conducted to define the extent of the outbreak. environmental cultures and ...201019694761
mycobacterial bacteraemia in patients infected and not infected with human immunodeficiency virus, taiwan.the clinical characteristics and outcomes of 71 patients with mycobacterial bacteraemia, infected with human immunodeficiency virus (hiv) (n = 47) and not infected with hiv (n = 24) are described. mycobacterium avium complex (mac) (54.9%) constituted the most frequently isolated mycobacterium, followed by mycobacterium tuberculosis (mtb) (38.0%), mycobacterium kansasii (4.2%), and mycobacterium abscessus (2.8%). the beijing family genotype was the most common type in mtb, and mycobacterium intra ...201019709128
fatal mycobacterium abscessus endocarditis misidentified as corynebacterium spp.the microbiological similarities of rapidly growing mycobacteria and corynebacteria impose potential for misidentification in the laboratory. this report describes a fatal case of infectious endocarditis caused by mycobacterium abscessus, initially identified and treated as corynebacterium spp. acid-fast staining should be performed routinely when multiple blood cultures grow corynebacterium spp.201019958237
carbapenem susceptibility patterns for clinical isolates of mycobacterium abscessus determined by the etest method.mycobacterium abscessus is resistant to multiple antibiotics, creating treatment challenges. carbapenems are tested to increase therapeutic alternatives. we performed in vitro susceptibility testing by etest of four carbapenems for m. abscessus isolates. imipenem demonstrated the most in vitro activity, and testing of other carbapenems provided no additional value.201020018813
otomastoiditis caused by mycobacterium abscessus, the netherlands. 201020031077
sequential bilateral lung resection in a patient with mycobacterium abscessus lung disease refractory to medical treatment.mycobacterium abscessus (m. abscessus) is the second most common nontuberculous mycobacteria (ntm) in south korea. nevertheless, the diagnosis and treatment of m. abscessus lung disease can be problematic. surgical resection has been tried for patients with localized m. abscessus lung disease refractory to medical treatment. here, we report on a 25-year-old woman with m. abscessus lung disease who had been diagnosed and treated three times for pulmonary tuberculosis. she was initially diagnosed ...201020046529
mycobacterium abscessus: a new player in the mycobacterial field.mycobacterium abscessus, a relative of koch's bacillus (the bacterium that causes tuberculosis), has recently emerged as the cause of an increasing number of both community- and hospital-acquired infections in humans; it also constitutes a serious threat for cystic fibrosis patients. this situation is worsened by its exceptionally high natural and acquired antibiotic resistance that complicates treatment. although a rapid grower, it shares some traits with koch's bacillus, including the ability ...201020060723
mycobacterium abscessus skin infection after tattooing: first case report and review of the literature.mycobacterium abscessus is a species of rapidly growing mycobacteria (rgm). this species can cause skin and soft tissue infections after trauma or surgical procedures, pulmonary infections and disseminated diseases in immunocompromised patients. it has been rarely documented after tattoo procedures. herein we describe the case of a 51-year- old man who presented with erythematous papules over a tattoo on the back 10 days after a tattoo session. culture revealed m. abscessus. tattoo infections, c ...201020558973
antimicrobial activity of mucosal-associated invariant t cells.mucosal-associated invariant t lymphocytes (mait lymphocytes) are characterized by two evolutionarily conserved features: an invariant t cell antigen receptor (tcr) alpha-chain and restriction by the major histocompatibility complex (mhc)-related protein mr1. here we show that mait cells were activated by cells infected with various strains of bacteria and yeast, but not cells infected with virus, in both humans and mice. this activation required cognate interaction between the invariant tcr and ...201020581831
chronic fistulas after breast augmentation secondary to mycobacterium abscessus. 201020595845
mycobacterium abscessus hand infections in immunocompetent fish handlers: case report.mycobacterium abscessus hand infections are rare and usually occur in immunocompromised patients or after injection with contaminated injectables. this article describes 2 cases of m abscessus infection of the hand in otherwise healthy fish handlers. mycobacterium abscessus can cause severe chronic tenosynovitis even in immunocompetent patients and should be suspected alongside the more common m marinum as a cause of nontuberculous mycobacterial hand infections in patients with aquatic and fish ...201020610060
molecular epidemiology of mycobacterium abscessus infections in a subtropical chronic ventilatory setting.the aim of this study was to investigate the high level of pulmonary mycobacterium abscessus infections and implement a surveillance programme among 43 ventilator-dependent patients, 15 with pulmonary m. abscessus infections, in a hospital long-term respiratory care ward (rcw) in central taiwan. m. abscessus isolates were obtained from 35 patients in the rcw of hospital a, 6 patients in the rcws of another three hospitals (b, c and d), and from 4 water sources in two of the hospitals (a and b). ...201020616186
antibiotic susceptibility pattern of rapidly growing mycobacteria.the rapidly growing mycobacteria (rgm) causing human infections primarily consist of the mycobacterium fortuitum group, mycobacterium abscessus and mycobacterium chelonae. the antibiotic susceptibility testing is important to determine the appropriate therapy as the antibiotics used to treat rgm are different from those used for treating infections caused by slow growers of mycobacteria.201020622384
comparison of real-time polymerase chain reaction and conventional biochemical methods for identification of mycobacterium chelonae-mycobacterium abscessus group to the species level.the mycobacterium chelonae-mycobacterium abscessus group (mcag) is the most common cause of infections because of rapidly growing mycobacteria. rapid identification of mcag to the species level is essential for choosing empiric antibiotic treatment and for public health measures. in this study, we compared the performance of a single-tube multiplex, real-time polymerase chain reaction (pcr) assay to 3 biochemical tests for species-level identification of 46 mcag isolates. we show that real-time ...201020638600
diagnosis of endocarditis caused by mycobacterium abscessus.we report a fatal case of native valve endocarditis due to mycobacterium abscessus in a hemodialysis patient. the diagnosis was based on culture isolation of acid-fast bacilli from peripheral blood and a permanent catheter tip, and their identification as m abscessus by a reverse hybridization-based assay and direct dna sequencing of the 16s-23s internal transcribed spacer region. rapid diagnosis and combination therapy are essential to minimize mortality due to this pathogen. although combinati ...201020697162
characterization and identification of distinct mycobacterium massiliense extracellular proteins from those of mycobacterium abscessus.mycobacterium massiliense is an emerging pathogen and very similar to mycobacterium abscessus of rapidly growing mycobacteria in the phenotype and genotype. pathogenic bacteria secrete a diversity of factors into extracellular medium which contribute to the bacterial pathogenicity. in the present study, we performed the comparative proteome analysis of culture filtrate proteins from a clinical isolate of m. massiliense and m. abscessus strains using two-dimensional gel electrophoresis and liquid ...201020799093
an oxidative environment promotes growth of mycobacterium abscessus.mycobacterium abscessus infections, particularly those causing chronic lung diseases, are becoming more prevalent worldwide. m. abscessus infections are difficult to treat because of antibiotic resistance. thus, new treatment options are urgently needed. m. abscessus is an intracellular pathogen that primarily infects macrophages and fibroblasts. because this bacterium has only recently been identified as a separate species, very little is known about m. abscessus-host interactions and how m. ab ...201020807564
rare complication after stripping operation: a case report of mycobacterium abscessus infection.mycobacterium abscessus is an acid-fast nontuberculous mycobacterium that grows rapidly in culture. the organism is found in dust, soil, and water and after trauma, it may infect skin and soft tissue. the organism is rarely found in humans, and infections occurring after cardiovascular surgery are rare clinical events. to our knowledge, only a few cases of hemodialysis arteriovenous graft infection and endocarditis caused by m. abscessus have been described. we reported a first case of patient w ...201023555416
an unusual surgical site infection in a liver transplant recipient.mycobacterium abscessus is a rare cause of human infection and is difficult to diagnose unless it is suspected. a 45-year-old woman underwent deceased donor liver transplantation following which she developed non-healing surgical site infection, which did not resolve with routine antibiotics for 2 months. the scraping of the wound revealed m abscessus infection. definitive identification of this species of mycobacterium was possible by its growth characteristics on culture and reverse line blot ...201022778369
successful treatment of a prosthetic joint infection due to mycobacterium abscessus.prosthetic joint infection due to mycobacterium abscessus is uncommon and optimal therapy remains poorly defined. following a two-stage revision, clinical and microbiological cure was achieved in a patient with a m abscessus-infected total hip arthroplasty. a prolonged course of directed antibacterial therapy comprising clarithromycin and cefoxitin coupled with the application of amikacin-impregnated cement likely contributed to the successful outcome.200920808461
community-acquired mastitis due to mycobacterium abscessus: a case report.mycobacterium abscessus is a rapidly growing mycobacterium usually causing skin and soft tissue infections in immunocompetent patients following contaminated traumatic or surgical wounds or contaminated injected medications. disseminated infections and pulmonary infections are usually reported in immunocompromised hosts.200920062768
[mycobacterium abscessus infection in insulin injection sites in a diabetic patient].insulin injection site abscesses have been attributed to pyogenic streptococci and staphylococcus aureus. in this report, the authors describe a patient in whom mycobacterium abscessus was isolated from one of multiple insulin injection sites. the article reviews six additional cases of rapidly growing mycobacteria identified as the causative agent of skin infections at insulin injection sites. in most cases, precise identification of the source of infection was not successful. possibly, inadequ ...200920027976
clinical relevance of mycobacterium chelonae-abscessus group isolation in 95 patients.to determine the clinical relevance of mycobacterium chelonae-abscessus group isolation from clinical samples.200919732795
a case of skin and soft tissue infection caused by mycobacterium abscessus.mycobacterium abscessus (m. abscessus) is an acid-fast bacillus that's classified as a pathogenic "rapid growing" nontuberculous mycobacteria. it is an uncommon cause of human disease, but it can cause skin and soft tissue infection after skin injury following inoculation, minor trauma and surgery. the single most important factor for determining the course and prognosis of a m. abscessus infection is the underlying immune status of the host. we report here on a 71-year-old female who presented ...200920548866
current treatment of atypical mycobacteriosis.atypical mycobacteria are a heterogeneous group of organisms that are of increasing importance because of the growing number of infections they cause. this rising rate of infection is due mainly to the increase in the number of susceptible (and especially immunosuppressed) patients.200919929702
venous catheter-associated bacteremia caused by rapidly growing mycobacteria at a medical center in central taiwan.infections due to rapidly growing mycobacteria (rgm) are increasing worldwide, especially in immunocompromised hosts, but data on the clinical features of patients with rgm bacteremia are limited in taiwan. this study was performed to determine the features associated with rgm.200919949759
mycobacterium abscessus infection complicating hand rejuvenation with structural fat grafting.summary: we report a case of mycobacterium abscessus infection of the hands in a 55 year old lady following hand rejuvenation with structural fat grafting. a review of the literature on m. abscessus infection to discuss common presentations, diagnosis and treatment is presented. awareness and high suspicion of such as atypical mycobacterial infections complicating hand surgery and other plastic surgery procedures is imperative to successful management.200918472321
acute respiratory failure involving an r variant of mycobacterium abscessus.we report the case of a cystic fibrosis patient colonized with a smooth-morphotype form of mycobacterium abscessus who developed acute respiratory failure with the emergence of an isogenic rough (r) variant while he was recovering from peritonitis-induced shock. this report emphasizes the role of r forms in severe m. abscessus infections.200919020061
[radiographic findings of pulmonary nontuberculous mycobacteriosis other than mycobacterium avium complex].almost all nontuberculous mycobacteria (ntm) cause opportunistic infection. therefore, the radiographic findings of ntm have a tendency of nonspecific patterns modifying the predisposing conditions or diseases and we could not extract species specific radiographic characterizations in that situations. in this review, the ntm cases without predisposing conditions or diseases are submitted essentially. mycobacterium kansasii cases show more or less the same patterns with tb cases. mycobacterium fo ...200919764463
extensive cutaneous mycobacterium abscessus infection due to contaminated insulin delivery system. 200919776153
molecular identification of rapidly growing mycobacteria isolates from pulmonary specimens of patients in the state of pará, amazon region, brazil.we isolated 44 strains of rapidly growing mycobacteria (rgm) from 19 patients with pulmonary infections assisted at the instituto evandro chagas (pará, brazil) from 2004 to 2007. identification at the species level was performed by pcr restriction fragment length polymorphism analysis (pra) of a 441 bp hsp65 fragment and partial 16s rrna, hsp65, and rpob gene sequencing. genotyping by pra yielded 3 digestion patterns: one identical to mycobacterium abscessus type i (group i); another to m. absce ...200919796904
high virulent clinical isolates of mycobacterium abscessus from patients with the upper lobe fibrocavitary form of pulmonary disease.pulmonary disease caused by nontuberculous mycobacteria (ntm), including mycobacterium abscessus, can be classified into two distinct types of clinical disease; the upper lobe fibrocavitary (uc) form and nodular bronchiectatic (nb) form. however, the relationship between mycobacterial strain virulence and disease type in the pulmonary m. abscessus diseases has not been reported. to determine the differential virulence between strains causing two forms of disease, we obtained clinical isolates fr ...200919800962
intrathoracic nontuberculous mycobacterial infections in otherwise healthy children.background: nontuberculous mycobacterial (ntm) infection is typically associated with lymphadenitis in immune competent children, and disseminated disease in children with immune deficiencies. isolated pulmonary ntm disease is seen in cystic fibrosis, and is increasingly recognized in immunocompetent elderly women, where it is associated with an increased incidence of cystic fibrosis transmembrane regulator (cftr) mutations. thoracic ntm infection has been reported rarely in otherwise healthy ch ...200919824053
non-tuberculous mycobacteria in children with cystic fibrosis: isolation, prevalence, and predictors.screening for non-tuberculous mycobacteria (ntm) is recommended for adults with cystic fibrosis (cf). the relevance of this organism in north american pediatric cf patients is unclear as there is limited ntm prevalence data for children. we aimed to determine the prevalence of ntm in children with cf from a single expectorated sputum and identify clinical predictors of ntm isolation. additionally, we compared two different sputum decontamination methods before mycobacterial culture.200919830845
multicenter study of prevalence of nontuberculous mycobacteria in patients with cystic fibrosis in france.we performed a multicenter prevalence study of nontuberculous mycobacteria (ntm) involving 1,582 patients (mean age, 18.9 years; male/female ratio, 1.06) with cystic fibrosis in france. the overall ntm prevalence (percentage of patients with at least one positive culture) was 6.6% (104/1,582 patients), with prevalences ranging from 3.7% (in the east of france) to 9.6% (in the greater paris area). mycobacterium abscessus complex (mabsc; 50 patients) and mycobacterium avium complex (mac; 23 patien ...200919846643
comparative analysis of immune responses to mycobacterium abscessus infection and its antigens in two murine models.mycobacterium abscessus has been identified as an emerging pulmonary pathogen in humans. because little is known regarding immune responses elicited by m. abscessus or its antigens, immunological responses were studied in two murine models subjected to intravenous (high-dose or systemic infection) or pulmonary (low-dose or local infection) inoculation with m. abscessus atcc 19977. an overall comparison between the two models showed similar patterns of bacterial survival and host immune responses ...200919851737
the antioxidant mimetic, mnte-2-pyp, reduces intracellular growth of mycobacterium abscessus.mycobacterium abscessus is a rapidly growing environmental mycobacterium that can cause severe skin, soft tissue, and lung infections. m. abscessus grows inside macrophages, and these cells release a vast number of proinflammatory cytokines in response to infections. the metalloporphyrin, mnte-2-pyp, is a broad antioxidant that reduces inflammatory cell signaling. macrophage-like thp-1 cells were infected with m. abscessus in the presence or absence of mnte-2-pyp. mnte-2-pyp significantly decrea ...200919097985
construction of mycobacterium abscessus defined glycopeptidolipid mutants: comparison of genetic tools.mycobacterium abscessus is a rapidly growing mycobacterial species that can be involved in pulmonary and disseminated infections in immunosuppressed or young cystic fibrosis patients. it is an emerging pathogen and has attracted recent attention due to the numerous cases of infection; furthermore, genomic tools have been developed for this species. nevertheless, the study of this species has until now been limited to spontaneous variants. we report here a comparison of three different mutagenesi ...200919114521
screening for sterilizing activity of antibiotic combinations in an acid model of rapidly growing mycobacteria during the stationary phase of growth.in view of the problems of correlation between the data provided by classical microbiological studies and clinical response, we designed an in vitro method to screen for the sterilizing activity of various antibiotics, individually or in combinations, against clinical isolates of various rapidly growing mycobacteria in the stationary phase.200919155619
a novel gene, erm(41), confers inducible macrolide resistance to clinical isolates of mycobacterium abscessus but is absent from mycobacterium chelonae.mycobacterium abscessus infections tend to respond poorly to macrolide-based chemotherapy, even though the organisms appear to be susceptible to clarithromycin. circumstantial evidence suggested that at least some m. abscessus isolates might be inducibly resistant to macrolides. thus, the purpose of this study was to investigate the macrolide phenotype of m. abscessus clinical isolates. inducible resistance to clarithromycin (mic > 32 microg/ml) was found for 7 of 10 clinical isolates of m. absc ...200919171799
lines of evidence for horizontal gene transfer of a phenazine producing operon into multiple bacterial species.phenazines are secondary metabolites with broad-spectrum antibiotic activity against bacteria, fungi, and eukaryotes. in pseudomonad species, a conserved seven-gene phenazine operon (phzabcdefg) is required for the conversion of chorismic acid to the broad-spectrum antibiotic phenazine-1-carboxylate. previous analyses of genes involved in phenazine production from nonpseudomonad species uncovered a high degree of sequence similarity to pseudomonad homologues. the analyses undertaken in this stud ...200919189039
immunoproteomic identification of secretory and subcellular protein antigens and functional evaluation of the secretome fraction of mycobacterium immunogenum, a newly recognized species of the mycobacterium chelonae-mycobacterium abscessus group.mycobacterium immunogenum has been associated with occupational pulmonary disease hypersensitivity pneumonitis (hp). the aim of this study was to identify immunogenic proteins (antigens) in this pathogen as a first step toward understanding its virulence factors and role in hp etiology. immunoproteomic profiling of secreted and subcellular protein fractions using a combination of two-dimensional electrophoresis (2-de), immunoblotting, and matrix-assisted laser desorption/ionization-time of fligh ...200919209886
hla antigens and nontuberculous mycobacterial lung disease in korean patients.human leukocyte antigen (hla) molecules are known to play an important role in host-defense mechanisms. the aim of this study was to evaluate the association between hla alleles and lung disease caused by nontuberculous mycobacteria (ntm) in korean patients.200919219503
mycobacterium abscessus post-injection abscesses from extrinsic contamination of multiple-dose bottles of normal saline in a rural clinic.we investigated an outbreak of gluteal abscesses following intramuscular (im) injections given at a clinic in rural china to identify the causative agent, source, and method of exposure.200919269204
unresolving furunculosis in a diabetic patient: cutaneous mycobacterium abscessus infection. 200919271048
mycobacterium abscessus from respiratory isolates: activities of drug combinations.mycobacterium abscessus is the most resistant of rapidly growing pathogenic mycobacteria. we performed a screening of the in vitro sterilizing capacity of various antibiotic combinations against two strains isolated from respiratory samples (in vitro activity of each antibiotic: 51 combinations of two drugs and 47 of three drugs). our findings confirm that clarithromycin is the most useful drug. the combination of this drug with linezolid exhibits good activity. in addition, the fluoroquinolones ...200919280301
successful treatment with pneumonectomy for pulmonary mycobacterium abscessus infection.we report a case of pulmonary mycobacterium abscessus (m. abscessus) infection with destructive growth in the entire right lung. the patient was 56-year-old woman who had had pulmonary tuberculosis at the age of 40 and had been diagnosed as having pulmonary mycobacterium abscessus infection 4 years prior to admission at our hospital. although various antibiotics were administered, persistent fever, hemoptysis and weight loss developed. after undergoing a right pneumonectomy, her clinical symptom ...200919293547
mycobacterium abscessus in cystic fibrosis lung transplant recipients: report of 2 cases and risk for recurrence.mycobacterium abscessus is increasingly recognized as an important pathogen in some individuals with advancing lung disease related to cystic fibrosis (cf). because of its resistance to antimicrobial agents and virulence, its presence in the lungs of potential lung transplant recipients can be problematic. we present 2 cases of individuals with cf in whom m. abscessus was present in the preoperative sputum cultures. the organism manifested different degrees of invasiveness in the 2 cases after t ...200919298240
microbiological spectrum of organisms isolated from explanted scleral buckles.scleral buckle removal is an uncommon procedure performed for various reasons. microbiological information on explanted scleral buckles remains limited. the authors identified 37 cases of scleral buckle removal during an 18-year period. bacterial cultures isolated an organism in 4 of 9 patients (44%) with clinical infection. organisms identified included methicillin-resistant staphylococcus aureus, pseudomonas aeruginosa, mycobacterium abscessus, and coagulase-negative staphylococcus species. ba ...200919320315
arteriovenous graft infection caused by mycobacterium abscessus in a hemodialysis patient. 200919356386
cohort study of molecular identification and typing of mycobacterium abscessus, mycobacterium massiliense, and mycobacterium bolletii.mycobacterium abscessus is the most common cause of rapidly growing mycobacterial chronic lung disease. recently, two new m. abscessus-related species, m. massiliense and m. bolletii, have been described. health care-associated outbreaks have recently been investigated by the use of molecular identification and typing tools; however, very little is known about the natural epidemiology and pathogenicity of m. massiliense or m. bolletii outside of outbreak situations. the differentiation of these ...200919420162
pulmonary nontuberculous mycobacterial infections: antibiotic treatment and associated costs.recent studies suggest an increasing prevalence of pulmonary nontuberculous mycobacteria (ntm) disease. in the absence of prevalence and cost data, the public health burden of pulmonary ntm disease is difficult to assess. the goal of this study was to assess costs associated with ntm disease treatment and to identify risk factors associated with increased costs. records from subjects with pulmonary ntm disease enrolled in a natural history protocol were abstracted for presenting symptoms, comorb ...200919467851
identification of non-tuberculous mycobacteria: utility of the genotype mycobacterium cm/as assay compared with hplc and 16s rrna gene sequencing.non-tuberculous mycobacteria (ntm) causing clinical disease have become increasingly common and more diverse. a new reverse line probe assay, genotype mycobacterium cm/as (hain lifescience), was evaluated for identification of a broad range of ntm. it was compared with phenotypic (hplc) and molecular (dna probes, in-house real-time multiplex species-specific pcr, 16s rrna gene pcr and sequencing) identification techniques, which together provided the reference 'gold standard'. a total of 131 cli ...200919502366
inaccuracy of single-target sequencing for discriminating species of the mycobacterium abscessus group.we determined nucleotide sequences of rpob, hsp65, and soda in 59 clinical isolates (from 58 patients) of the mycobacterium abscessus group. identification to the species level, based on three target genes, was concordant for 44 isolates (25 m. abscessus, 13 mycobacterium massiliense, and 6 mycobacterium bolletii isolates) and discordant for 15 isolates which had "interspecific composite patterns." sequence analysis of five housekeeping genes also showed composite patterns in 8 of these 15 isola ...200919515839
comparison of clinical and radiographic characteristics between nodular bronchiectatic form of nontuberculous mycobacterial lung disease and diffuse panbronchiolitis.the nodular bronchiectatic form of nontuberculous mycobacterial (ntm) lung disease and diffuse panbronchiolits (dpb) show similar clinical and radiographic findings. the present study was performed to clarify the clinicoradiographic similarities as well as the differences between ntm lung disease and dpb. the initial clinicoradiographic features of 78 patients with the nodular bronchiectatic form of ntm lung disease (41 patients with mycobacterium avium complex infection and 37 patients with myc ...200919543504
non mycobacterial virulence genes in the genome of the emerging pathogen mycobacterium abscessus.mycobacterium abscessus is an emerging rapidly growing mycobacterium (rgm) causing a pseudotuberculous lung disease to which patients with cystic fibrosis (cf) are particularly susceptible. we report here its complete genome sequence. the genome of m. abscessus (cip 104536t) consists of a 5,067,172-bp circular chromosome including 4920 predicted coding sequences (cds), an 81-kb full-length prophage and 5 is elements, and a 23-kb mercury resistance plasmid almost identical to pmm23 from mycobacte ...200919543527
nontuberculous mycobacteria isolated during the treatment of pulmonary tuberculosis.the significance of nontuberculous mycobacteria (ntm) isolated from a patient during therapy for pulmonary tuberculosis (tb) is uncertain. we investigated the frequency and clinical significance of ntm isolated from patients receiving anti-tb treatment.200919576745
femoropopliteal prosthetic bypass graft infection due to mycobacterium abscessus localized by fdg-pet/ct scan.a patient with postvascular graft placement presented with bacteremia but no localizing symptoms. our standard infected graft workup of computed tomography (ct) scan, ultrasound scan, magnetic resonance imaging (mri) scan, and additional laboratory tests did not localize the infection source. nuclear medicine had three options including white blood cell (wbc) scan, gallium scan, and the fluorine-18-fluorodeoxyglucose positron emission tomography (fdg-pet)/ct scan. fdg-pet/ct imaging alone demons ...200919595547
mycobacterium abscessus glycopeptidolipids mask underlying cell wall phosphatidyl-myo-inositol mannosides blocking induction of human macrophage tnf-alpha by preventing interaction with tlr2.mycobacterium abscessus causes disease in patients with structural abnormalities of the lung, and it is an emerging pathogen in patients with cystic fibrosis. colonization of the airways by nontuberculous mycobacteria is a harbinger of invasive lung disease. colonization is facilitated by biofilm formation, with m. abscessus glycopeptidolipids playing an important role. m. abscessus can transition between a noninvasive, biofilm-forming, smooth colony phenotype that expresses glycopeptidolipid, a ...200919596998
antibiotic treatment of mycobacterium abscessus lung disease: a retrospective analysis of 65 patients.the optimal therapeutic regimen and duration of treatment for mycobacterium abscessus lung disease is not well established.200919661243
detection of lfra and tap efflux pump genes among clinical isolates of non-pigmented rapidly growing mycobacteria.this study was performed to detect lfra and tap efflux pumps among clinical isolates of non-pigmented rapidly growing mycobacteria (nprgm). gene detection was performed using polymerase chain reaction (pcr) with specific primers designed for each gene. susceptibility of the strains to doxycycline, tigecycline and ciprofloxacin was analysed using the broth microdilution reference technique. in total, 166 clinical isolates were included in the study. the lfra gene was detected in four strains (2.4 ...200919665358
mycobacterium abscessus infection in solid organ transplant recipients: report of three cases and review of the literature.mycobacterium abscessus is an ubiquitous organism found in the environment. this rapidly growing mycobacterium infrequently causes disease in humans; however, in immunocompromised hosts, disease can range from localized cutaneous lesions to disseminated infection. the organism is resistant to most antimycobacterial drugs and therapy can be limited by drug interactions. the exact incidence of m. abscessus infection among solid organ transplant (sot) recipients is unknown; data are only available ...200919671119
localised pyogranulomatous dermatitis due to mycobacterium abscessus in a cat: a case report.a case of pyogranulomatous dermatitis, caused by mycobacterium abscessus, an unusual opportunistic mycobacterium spp., is described in a cat. histopathological examination of the affected skin confirmed the diagnosis and ziehl-neelsen staining revealed acid-fast rods. a rapidly growing mycobacterium was found after culture on a löwenstein-jensen medium. real-time polymerase chain reaction for the 16s rdna (434bp) sequence and the sequence of the rpob gene (359bp) revealed 99% and 100% matches, r ...200918083608
mycobacterium abscessus infection after breast augmentation: a case of contaminated implants?infections after breast augmentation are uncommon, occurring in 1-3% of cases. treatment often requires additional surgeries and may yield a sub-optimal cosmetic result. for this reason, post implant infection remains a serious concern among plastic surgeons. a 48-year-old female presented to our clinic with bilateral breast implant infections 3 months after primary augmentation in china. cultures grew mycobacterium abscessus, a previously undescribed infectious aetiology after breast augmentati ...200918485852
rapid speciation of 15 clinically relevant mycobacteria with simultaneous detection of resistance to rifampin, isoniazid, and streptomycin in mycobacterium tuberculosis complex.to design and standardize an in-house reverse line blot hybridization (rlbh) assay for the accurate identification of 15 clinically relevant species of mycobacteria and for the detection of drug resistance to rifampin (rif), isoniazid (inh), and streptomycin (str) in mycobacterium tuberculosis complex (mtb).200918565777
[rapidly growing mycobacteria infection after videosurgical procedures - the glutaraldehyde hypothesis].between august 2006 and february 2007, in the state of rio de janeiro, brazil, a massive outbreak of rgm infections after video laparoscopy was mainly associated to the recently described mycobacterium massiliense species. all confirmed and probable cases reports described the use of high-level disinfection of medical devices by using 2% glutaraldehyde (2% ga) for 30 min before the surgical procedures. we investigated the susceptibility of the m. massiliense isolates recovered during the outbrea ...200920076909
mastoiditis secondary to mycobacterium abscessus imaged with gallium-67 scintigraphy.atypical mycobacterium is rarely seen as a cause of chronic mastoiditis but has been increasingly recognized over the past few years. mycobacterium abscessus is the most pathogenic and chemotherapy-resistant, rapid-growing mycobacterium of all the four groups. this paper presents a case of a 57-year-old woman who had chronic mastoiditis with recurrent exacerbations. the initial computed tomography (ct) findings showed the presence of an inflammatory process and she was treated with the appropria ...200821614326
an autopsy case of mycobacterium abscessus pulmonary infection complicated with rheumatoid arthritis.we present the case of a 70-year-old man diagnosed with mycobacterium abscessus pulmonary infection complicated with rheumatoid arthritis who was treated with corticosteroids. he died despite treatment according to the recommended regimen of imipenem, clarithromycin, and amikacin. autopsy revealed granulomatous lesions throughout the bilateral lungs. we conclude that m. abscessus infection may have a fatal outcome because of the drug resistance of the pathogen. this case suggests that rheumatoid ...200818591855
association between microsatellite polymorphisms in intron ii of the human toll-like receptor 2 gene and nontuberculous mycobacterial lung disease in a korean population.this study evaluated the association between the guanine-thymine (gt) repeat polymorphism in intron ii of the toll-like receptor 2 (tlr2) gene and lung disease caused by nontuberculous mycobacteria (ntm). polymerase chain reaction and gene scans were used to determine the numbers of gt repeats for 193 patients with the nodular bronchiectatic form of ntm lung disease, including 110 patients with mycobacterium avium-intracellulare (mac) infection, 82 patients with mycobacterium abscessus infection ...200818602432
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