Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
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| development and evaluation of polymerase chain reaction assay to detect burkholderia genus and to differentiate the species in clinical specimens. | molecular-based techniques are becoming desirable as tools for identification of infectious diseases. amongst the burkholderia spp., there is a need to differentiate burkholderia pseudomallei from burkholderia cepacia, as misidentification could lead to false treatment of patients. in this study, conventional pcr assay targeting three genes was developed. primers were designed for the amplification of burkholderia genus-specific groel gene, b. pseudomallei-specific mpra gene and b. cepacia-speci ... | 2010 | 20345378 |
| identification of a regulatory cascade controlling type iii secretion system 3 gene expression in burkholderia pseudomallei. | a major and critical virulence determinant of many gram-negative bacterial pathogens is the type iii secretion systems (t3ss). t3ss3 in burkholderia pseudomallei is critical for bacterial virulence in mammalian infection models but its regulation is unknown. b. pseudomallei is the causative agent of melioidosis, a potentially fatal disease endemic in southeast asia and northern australia. while screening for bacterial transposon mutants with a defective t3ss function, we discovered a tetr family ... | 2010 | 20345664 |
| construction of aminoglycoside-sensitive burkholderia cenocepacia strains for use in studies of intracellular bacteria with the gentamicin protection assay. | burkholderia cenocepacia is a multidrug-resistant opportunistic pathogen that infects the airways of patients with cystic fibrosis (cf) and can survive intracellularly in macrophages and epithelial cells. the gentamicin protection assay, which relies on the poor ability of gentamicin or other aminoglycosides to permeate eukaryotic cell membranes, is traditionally employed to quantify intracellular bacteria. however, the high resistance of these bacteria to aminoglycosides hampers the use of the ... | 2010 | 20348312 |
| complex bacterial infections pre- and posttransplant. | infections complications following lung transplantation are associated with significant morbidity and mortality. management of infections is most challenging in patients with cystic fibrosis (cf), but all lung transplant recipients are at heightened risk for opportunistic infections. particularly in cf, pretransplant infections with pseudomonas aeruginosa, other highly resistant bacteria (e.g., stenotrophomonas, burkholderia), and mycobacteria play a major role in recipient selection and post-lu ... | 2010 | 20354935 |
| susceptibility of burkholderia pseudomallei to tigecycline and other antimicrobials. | 2010 | 20356698 | |
| the man with the boggy head: cranial melioidosis. | melioidosis is known to cause abscesses in various organs, including the cranium, though less commonly. we present a patient with scalp abscess and subdural empyema that was visible on computed tomography of the brain. the neurosurgical drainage grew burkholderia pseudomallei. despite our best effort to treat the patient using parenteral antibiotics and surgical drainage, the patient did not survive. | 2010 | 20358143 |
| an outbreak of burkholderia cenocepacia bacteremia in immunocompromised oncology patients. | burkholderia cepacia is a common environmental bacterium that is resistant to disinfectants, and therefore is often encountered as a hospital-acquired pathogen. we describe an outbreak of b. cenocepacia bacteremia among hospitalized oncology patients. | 2010 | 20358245 |
| p-nitrophenol degradation via 4-nitrocatechol in burkholderia sp. sj98 and cloning of some of the lower pathway genes. | microbial degradation studies have pointed toward the occurrence of two distinct pnp catabolic pathways in gram positive and gram negative bacteria. the former involves 4-nitrocatechol (4-nc), 1,2,4-benzenetriol (bt), and maleylacetate (ma) as major degradation intermediates, whereas the later proceeds via formation of 1,4-benzoquinone (bq) and hydroquinone (hq). in the present study we identified a gram negative organism viz. burkholderia sp. strain sj98 that degrades pnp via 4nc, bt, and ma. a ... | 2010 | 20359211 |
| bovine lactoferrin interacts with cable pili of burkholderia cenocepacia. | in this study we evaluated the ability of lactoferrin, the most abundant antimicrobial protein in airway secretions, to bind the surface structures of a burkholderia strain cystic fibrosis-isolated. burkholderia cenocepacia is a gram-negative bacterium involved as respiratory pathogen in cystic fibrosis patient infections. this bacterium possesses filamentous structures, named cable pili that have been proposed as virulence factors because of their ability to bind to respiratory epithelia and mu ... | 2010 | 20364433 |
| a genomic survey of positive selection in burkholderia pseudomallei provides insights into the evolution of accidental virulence. | certain environmental microorganisms can cause severe human infections, even in the absence of an obvious requirement for transition through an animal host for replication ("accidental virulence"). to understand this process, we compared eleven isolate genomes of burkholderia pseudomallei (bp), a tropical soil microbe and causative agent of the human and animal disease melioidosis. we found evidence for the existence of several new genes in the bp reference genome, identifying 282 novel genes su ... | 2010 | 20368977 |
| why genes evolve faster on secondary chromosomes in bacteria. | in bacterial genomes composed of more than one chromosome, one replicon is typically larger, harbors more essential genes than the others, and is considered primary. the greater variability of secondary chromosomes among related taxa has led to the theory that they serve as an accessory genome for specific niches or conditions. by this rationale, purifying selection should be weaker on genes on secondary chromosomes because of their reduced necessity or usage. to test this hypothesis we selected ... | 2010 | 20369015 |
| effects of pesticides on the bacterial production of pyrrolnitrin. | pyrrolnitrin is a halogenated bacterial metabolite with antifungal and antibacterial activities which served as a lead structure of synthetic fungicides. several pyrrolnitrin-producing bacteria are considered to be promising biopesticides. however, the application of these microorganisms is not straightforward since many synthetic pesticides usually coexist in agricultural fields and inevitably affect the efficacy of biocontrol agents. in this regard, effects of 25 xenobiotics, including 18 pest ... | 2010 | 20373823 |
| co-swarming and local collapse: quorum sensing conveys resilience to bacterial communities by localizing cheater mutants in pseudomonas aeruginosa. | members of swarming bacterial consortia compete for nutrients but also use a co-operation mechanism called quorum sensing (qs) that relies on chemical signals as well as other secreted products ("public goods") necessary for swarming. deleting various genes of this machinery leads to cheater mutants impaired in various aspects of swarming cooperation. | 2010 | 20376321 |
| role of bacteriocins in mediating interactions of bacterial isolates taken from cystic fibrosis patients. | pseudomonas aeruginosa (pa) and burkholderia cepacia complex (bcc) lung infections are responsible for much of the mortality in cystic fibrosis (cf). however, little is known about the ecological interactions between these two, often co-infecting, species. this study provides what is believed to be the first report of the intra- and interspecies bacteriocin-like inhibition potential of pa and bcc strains recovered from cf patients. a total of 66 strains were screened, and shown to possess bacter ... | 2010 | 20378653 |
| huanglongbing, a systemic disease, restructures the bacterial community associated with citrus roots. | to examine the effect of pathogens on the diversity and structure of plant-associated bacterial communities, we carried out a molecular analysis using citrus and huanglongbing as a host-disease model. 16s rrna gene clone library analysis of citrus roots revealed shifts in microbial diversity in response to pathogen infection. the clone library of the uninfected root samples has a majority of phylotypes showing similarity to well-known plant growth-promoting bacteria, including caulobacter, burkh ... | 2010 | 20382817 |
| rational creation of mutant enzyme showing remarkable enhancement of catalytic activity and enantioselectivity toward poor substrates. | catalytic activity and enantioselectivity of lipase toward poor substrates bearing bulky substituents on both sides have been dramatically improved by rational design; the e value for a poor substrate was increased from 5 (wild-type enzyme) to >200 (i287f/i290a double mutant) with an acceleration of the reaction rate. | 2010 | 20383389 |
| pathogenicity, virulence factors, and strategies to fight against burkholderia cepacia complex pathogens and related species. | the burkholderia cepacia complex (bcc) is a group of 17 closely related species of the beta-proteobacteria subdivision that emerged in the 1980s as important human pathogens, especially to patients suffering from cystic fibrosis. since then, a remarkable progress has been achieved on the taxonomy and molecular identification of these bacteria. although some progress have been achieved on the knowledge of the pathogenesis traits and virulence factors used by these bacteria, further work envisagin ... | 2010 | 20390415 |
| application of electro-active biofilms. | the concept of an electro-active biofilm (eab) has recently emerged from a few studies that discovered that certain bacteria which form biofilms on conductive materials can achieve a direct electrochemical connection with the electrode surface using it as electron exchanger, without the aid of mediators. this electro-catalytic property of biofilms has been clearly related to the presence of some specific strains that are able to exchange electrons with solid substrata (eg geobacter sulfurreducen ... | 2010 | 20390557 |
| preliminary characterization of biosurfactants produced by microorganisms isolated from refinery wastewaters. | some bacterial strains isolated from refinery wastewaters were identified as pseudomonas aeruginosa rwi, pseudomonas putida rwii, pseudomonas fluorescens rwiii and burkholderia cepacia rwiv, and the biosurfactants produced by these strains were coded as bs-i, bs-ii, bs-iii and bs-iv, respectively. the bacterial strains were characterized by the following biochemical methods: gram stain, oxidase activity, indol, lactose and growth at 42 degrees c. biosurfactant production was evaluated by: emulsi ... | 2010 | 20391807 |
| efflux pumps may play a role in tigecycline resistance in burkholderia species. | the purpose of this study was to investigate the role of multidrug resistance efflux pumps in relation to decreased susceptibility to tigecycline in clinical isolates of burkholderia cepacia complex (bcc). the role of efflux pumps was analysed using the efflux pump inhibitor (epi) mc-207,110. minimum inhibitory concentrations (mics) were determined for each strain against tigecycline alone and in the presence of 64 mg/l mc-207,110. the effect of efflux pump inhibition on the susceptibility of bc ... | 2010 | 20399621 |
| methods to determine fitness in bacteria. | acquisition of antibiotic resistance may be associated with a physiological cost for the bacterium. determination of growth rate and generation time is often used to measure fitness costs associated with antibiotic resistance. however, fitness costs may be small and difficult to quantify and multiple models are required. available in vitro models that can be used to measure fitness include quantification of biofilm growth, survival in water, resistance to drying, and measurement of planktonic gr ... | 2010 | 20401590 |
| a burkholderia pseudomallei deltapurm mutant is avirulent in immunocompetent and immunodeficient animals: candidate strain for exclusion from select-agent lists. | burkholderia pseudomallei causes the disease melioidosis in humans and is classified as a category b select agent. research utilizing this pathogen is highly regulated in the united states, and even basic studies must be conducted in biosafety level 3 (bsl-3) facilities. there is currently no attenuated b. pseudomallei strain available that is excluded from select-agent regulations and can be safely handled at bsl-2 facilities. to address this need, we created bp82 and bp190, which are deltapurm ... | 2010 | 20404077 |
| evaluation of igm elisa using a sonicate and a lipopolysaccharide antigen for the serodiagnosis of melioidosis. | melioidosis, caused by burkholderia pseudomallei, has variable manifestations. the disease can present as an acute or a chronic form or localized or disseminated or can remain latent for many years. acute septicaemic melioidosis has a high fatality rate when untreated and therefore, an early diagnosis is critical. lack of testing facilities and of an awareness of the manifestations of the disease makes it likely that it is underreported in india. a sonicate and a lipopolysaccharide (lps) antigen ... | 2010 | 20404465 |
| adhesion and biofilm formation on polystyrene by drinking water-isolated bacteria. | this study was performed in order to characterize the relationship between adhesion and biofilm formation abilities of drinking water-isolated bacteria (acinetobacter calcoaceticus, burkholderia cepacia, methylobacterium sp., mycobacterium mucogenicum, sphingomonas capsulata and staphylococcus sp.). adhesion was assessed by two distinct methods: thermodynamic prediction of adhesion potential by quantifying hydrophobicity and the free energy of adhesion; and by microtiter plate assays. biofilms w ... | 2010 | 20405208 |
| proteomic analysis of quorum sensing-dependent proteins in burkholderia glumae. | burkholderia glumae, the causal agent of bacterial rice grain rot, utilizes quorum sensing (qs) systems that rely on n-octanoyl homoserine lactone (synthesized by tofi) and its cognate receptor tofr to activate toxoflavin biosynthesis genes and an iclr-type transcriptional regulator gene, qsmr. since qs is essential for b. glumae pathogenicity, we analyzed the qs-dependent proteome by 2-dimensional gel electrophoresis. a total of 79 proteins, including previously known qs-dependent proteins, wer ... | 2010 | 20408571 |
| isolation and characterisation of the biological repeating unit of cepacian, the exopolysaccharide produced by bacteria of the burkholderia cepacia complex. | the repeating unit of cepacian, the exopolysaccharide produced by the majority of the microorganisms belonging to the burkholderia cepacia complex, was isolated from inner bacterial membranes and investigated by mass spectrometry, with and without prior derivatisation. interpretation of the mass spectra led to the determination of the biological repeating unit primary structure, thus disclosing the nature of the oligosaccharide produced in vivo. moreover, mass spectra recorded on the native samp ... | 2010 | 20409536 |
| identification of burkholderia multivorans atcc 17616 genes induced in soil environment by in vivo expression technology. | burkholderia multivorans atcc 17616 was originally isolated from a soil sample, and it carries three chromosomes. to identify traits of likely adaptive significance for colonization of soil, an in vivo expression technology system for atcc 17616 was constructed using the promoterless and tandemly arranged dapb and lacz genes as the reporters, and this system was applied to identify the genomic loci of atcc 17616 that were induced in sterilized soil. our screening of a library consisting of dapb- ... | 2010 | 20412282 |
| [tropical pneumonia: pulmonary melioidosis]. | melioidosis is a disease that is endemic in north australia and southeast asia, caused by burkholderia pseudomallei. this gram-negative organism can affect all organs, but presents most commonly as severe pneumonia. we report a 58-year-old man who presented with a b. pseudomallei pneumonia on his return from thailand. radiography revealed a complete disappearance of symptoms after a 3-week therapy with ceftazidime and doxycycline followed by 4 months of combined amoxicillin-clavulanic acid and d ... | 2010 | 20413195 |
| recovery and screening for antibiotic susceptibility of potential bacterial pathogens from the oral cavity of shark species involved in attacks on humans in recife, brazil. | the number of incidents involving sharks and humans at beaches in recife, on the north-eastern brazilian coast, is among the highest worldwide. in addition, wound infections in survivors are common; but the nature and risk of the aetiological agents is unknown. in the present study, 81 potential bacterial pathogens were identified in the oral cavity of sharks involved in attacks in recife, and were subjected to antibiotic susceptibility tests using the standardized disc-diffusion method. the maj ... | 2010 | 20413619 |
| mineralization of pcbs by the genetically modified strain cupriavidus necator jms34 and its application for bioremediation of pcbs in soil. | polychlorobiphenyls (pcbs) are classified as "high-priority pollutants." diverse microorganisms are able to degrade pcbs. however, bacterial degradation of pcbs is generally incomplete, leading to the accumulation of chlorobenzoates (cbas) as dead-end metabolites. to obtain a microorganism able to mineralize pcb congeners, the bph locus of burkholderia xenovorans lb400, which encodes one of the most effective pcb degradation pathways, was incorporated into the genome of the cba-degrading bacteri ... | 2010 | 20414654 |
| burkholderia pseudomallei musculoskeletal infections (melioidosis) in india. | melioidosis, an infection due to gram negative burkholderia pseudomallei, is an important cause of sepsis in east asia especially thailand and northern australia. it usually causes abscesses in lung, liver, spleen, skeletal muscle and parotids especially in patients with diabetes, chronic renal failure and thalassemia. musculoskeletal melioidosis is not common in india even though sporadic cases have been reported mostly involving soft tissues. during a two-year-period, we had five patients with ... | 2010 | 20419012 |
| promoting fertilizer use via controlled release of a bacteria-encapsulated film bag. | a phosphate-solubilizing bacterium ( burkholderia cepacia isolate) encapsulated in maleic anhydride (ma) grafted onto poly(butylene succinate adipate) (pbsa) and then combined with starch as film bag material (pbsa-g-ma/starch) incubated in a saline solution required approximately 20 days to deplete the starch in the film bags. thereafter, the cell concentration in the saline solution increased significantly because of the release of cells from the severely destroyed film bags and also their gro ... | 2010 | 20420423 |
| screening of lipases for the synthesis of xylitol monoesters by chemoenzymatic esterification and the potential of microwave and ultrasound irradiations to enhance the reaction rate. | lipases from different sources, pseudomonas fluorescens (ak lipase), burkholderia cepacia (ps lipase), penicillium camembertii (lipase g) and porcine pancreas lipase (ppl), previously immobilized on epoxy sio(2)-pva, were screened for the synthesis of xylitol monoesters by esterification of the protected xylitol using oleic acid as acyl donor group. among all immobilized derivatives, the highest esterification yield was achieved by p. camembertii lipase, showing to be attractive alternative to b ... | 2010 | 20420851 |
| fatal burkholderia pseudomallei septicaemia in a patient with diabetes. | 2010 | 20424312 | |
| immobilization of pseudomonas cepacia lipase onto electrospun polyacrylonitrile fibers through physical adsorption and application to transesterification in nonaqueous solvent. | the lipase of pseudomonas cepacia was immobilized onto electrospun polyacrylonitrile (pan) fibers and used for the conversion of (s)-glycidol with vinyl n-butyrate to glycidyl n-butyrate in isooctane. the rate of reaction with the adsorbed lipase was 23-fold higher than the initial material. after 10 recyclings, the initial reaction rate was 80% of the original rate. this system of enzyme immobilization is therefore suitable for carrying out transesterification reactions in nonaqueous solvents. | 2010 | 20424890 |
| pseudo outbreak of burkholderia cepacia in vaginal cultures and intervention by hospital infection control team. | 2010 | 20434797 | |
| commonalities and differences in regulation of n-acyl homoserine lactone quorum sensing in the beneficial plant-associated burkholderia species cluster. | the genus burkholderia includes over 60 species isolated from a wide range of environmental niches and can be tentatively divided into two major species clusters. the first cluster includes pathogens such as burkholderia glumae, b. pseudomallei, and b. mallei and 17 well-studied species of the burkholderia cepacia complex. the other recently established cluster comprises at least 29 nonpathogenic species, which in most cases have been found to be associated with plants. it was previously establi ... | 2010 | 20435760 |
| beyond antibiotics: new horizons in treating burkholderia species infections. | 2010 | 20438351 | |
| antisense phosphorodiamidate morpholino oligomers targeted to an essential gene inhibit burkholderia cepacia complex. | members of the burkholderia cepacia complex (bcc) cause considerable morbidity and mortality in patients with chronic granulomatous disease and cystic fibrosis. many bcc strains are antibiotic resistant, which requires the exploration of novel antimicrobial approaches, including antisense technologies such as phosphorodiamidate morpholino oligomers (pmos). | 2010 | 20438352 |
| disseminated penicilliosis, recurrent bacteremic nontyphoidal salmonellosis, and burkholderiosis associated with acquired immunodeficiency due to autoantibody against gamma interferon. | acquired immunodeficiency due to autoantibody against gamma interferon has recently been associated with opportunistic nontuberculous mycobacteriosis, especially among southeast asians. we report another 8 cases, all except one apparently immunocompetent hosts who suffered from concomitant or sequential infections by other intracellular pathogens causing penicilliosis, extraintestinal nontyphoidal salmonellosis, and burkholderiosis. the only case with an underlying immunodeficiency syndrome had ... | 2010 | 20445006 |
| the structure of sedoheptulose-7-phosphate isomerase from burkholderia pseudomallei reveals a zinc binding site at the heart of the active site. | heptoses are found in the surface polysaccharides of most bacteria, contributing to structures that are essential for virulence and antibiotic resistance. consequently, the biosynthetic enzymes for these sugars are attractive targets for novel antibiotics. the best characterized biosynthetic enzyme is gmha, which catalyzes the conversion of sedoheptulose-7-phosphate into d-glycero-d-manno-heptopyranose-7-phosphate, the first step in the biosynthesis of heptose. here, the structure of gmha from b ... | 2010 | 20447408 |
| nodulation and nitrogen fixation by mimosa spp. in the cerrado and caatinga biomes of brazil. | *an extensive survey of nodulation in the legume genus mimosa was undertaken in two major biomes in brazil, the cerrado and the caatinga, in both of which there are high degrees of endemicity of the genus. *nodules were collected from 67 of the 70 mimosa spp. found. thirteen of the species were newly reported as nodulating. nodules were examined by light and electron microscopy, and all except for m. gatesiae had a structure typical of effective mimosa nodules. the endosymbiotic bacteria in nodu ... | 2010 | 20456044 |
| [melioidosis: the importance of a detailed medical history, including recent travels]. | a 70-year-old woman was admitted to hospital with fever and signs of bronchopneumonia following a recent visit to southeast asia. she was diagnosed with melioidosis and treated with ceftazidime i.v. for two weeks, followed by oral co-trimoxazol and folinic acid. she recovered and had no recurring disease in the first year following recovery. melioidosis is caused by an infection with burkholderia pseudomallei. clinical presentation can vary, but pneumonia is present in most patients. the diagnos ... | 2010 | 20456806 |
| enrichment and cultivation of pelagic bacteria from a humic lake using phenol and humic matter additions. | individual bacterial populations are known to respond differently toward substrate availability. to test how the availability of either pure phenol or natural humic matter (hm) selects for specific pelagic bacteria phylotypes from a humic lake (lake grosse fuchskuhle, northeastern germany), we used culture-dependent and -independent approaches. using a batch approach, the bacterial community composition (bcc) differed depending on both the quantity and the quality of added substrates. using a di ... | 2010 | 20459514 |
| endophytic bacteria improve phytoremediation of ni and tce co-contamination. | the aim of this work was to investigate if engineered endophytes can improve phytoremediation of co-contaminations by organic pollutants and toxic metals. as a model system, yellow lupine was inoculated with the endophyte burkholderia cepacia vm1468 possessing (a) the ptom-bu61 plasmid, coding for constitutive trichloroethylene (tce) degradation, and (b) the ncc-nre ni resistance/sequestration system. plants were exposed to ni and tce and (a) ni and tce phytotoxicity, (b) tce degradation and eva ... | 2010 | 20462680 |
| evaluation of azithromycin, trovafloxacin and grepafloxacin as prophylaxis for experimental murine melioidosis. | the efficacies of the azalide azithromycin and the fluoroquinolones trovafloxacin and grepafloxacin for pre- and post-exposure prophylaxis of infection with high or low challenge doses of burkholderia pseudomallei strain 576 were assessed in an experimental mouse model. trovafloxacin and grepafloxacin afforded significant levels of protection, whereas azithromycin was ineffective and potentially detrimental. overall, the data suggest that some fluoroquinolones may have potential utility in proph ... | 2010 | 20462743 |
| failure to recover to baseline pulmonary function after cystic fibrosis pulmonary exacerbation. | patients with cystic fibrosis periodically experience pulmonary exacerbations. previous studies have noted that some patients' lung function (fev(1)) does not improve with treatment. | 2010 | 20463179 |
| cluster of pseudoinfections with burkholderia cepacia associated with a contaminated washer-disinfector in a bronchoscopy unit. | in december 2008, bronchoalveolar lavage fluid samples obtained from 3 patients were positive for burkholderia cepacia complex on culture. samples obtained from bronchoscopes and rinse-water samples obtained from the washer-disinfector were found to be positive for b. cepacia complex. the cause of this pseudo-outbreak was that the washer-disinfector was installed without the required antibacterial filter. | 2010 | 20470036 |
| enhancing activity and stability of burkholderia cepacia lipase by immobilization on surface-functionalized mesoporous silicates. | burkholderia cepacia lipase was immobilized on various types of phenyl-functionalized mesoporous silicates (mps). mps, anchored with a phenyl group on the silica wall and with three dimensional (3d) mesoporosity, showed highest lipase adsorption capacity and best activities both in aqueous and organic reagents. | 2010 | 20471602 |
| characterization of the n-atpase, a distinct, laterally transferred na+-translocating form of the bacterial f-type membrane atpase. | an analysis of the distribution of the na(+)-translocating atpases/atp synthases among microbial genomes identified an atypical form of the f(1)f(o)-type atpase that is present in the archaea methanosarcina barkeri and m. acetivorans, in a number of phylogenetically diverse marine and halotolerant bacteria and in pathogens burkholderia spp. in complete genomes, representatives of this form (referred to here as n-atpase) are always present as second copies, in addition to the typical proton-trans ... | 2010 | 20472544 |
| burkholderia phymatum strains capable of nodulating phaseolus vulgaris are present in moroccan soils. | phylogenetic analysis of 16s rrna, nodc, and nifh genes of four bacterial strains isolated from root nodules of phaseolus vulgaris grown in morocco soils were identified as burkholderia phymatum. all four strains formed n(2)-fixing nodules on p. vulgaris and mimosa, acacia, and prosopis species and reduced acetylene to ethylene when cultured ex planta. | 2010 | 20472732 |
| pulmonary melioidosis in febrile neutropenia: the rare and deadly duet. | we present the first two fulminant cases of pulmonary melioidosis in febrile neutropenic patients with acute and varied presentations seen in our institution and their fatal outcome. a high index of suspicion coupled with microbiological confirmation can facilitate the administration of the appropriate therapy in cases of melioidosis that differ from other bacterial infections in terms of presentation and the response to antimicrobials. | 2010 | 20478984 |
| meropenem dosing in critically ill patients with sepsis receiving high-volume continuous venovenous hemofiltration. | use of high ultrafiltrate flow rates with continuous venovenous hemofiltration (cvvhf) in critically ill patients is an emerging setting, for which there are few data to guide drug dosing. the objectives of this study were, firstly, to investigate the pharmacokinetics of meropenem in critically ill patients with severe sepsis who are receiving high-volume cvvhf with high-volume exchanges (> or = 4 liters/h); secondly, to determine whether standard dosing regimens (1,000 mg intravenously [i.v.] e ... | 2010 | 20479205 |
| evaluation of surrogate animal models of melioidosis. | burkholderia pseudomallei is the gram-negative bacterial pathogen responsible for the disease melioidosis. b. pseudomallei establishes disease in susceptible individuals through multiple routes of infection, all of which may proceed to a septicemic disease associated with a high mortality rate. b. pseudomallei opportunistically infects humans and a wide range of animals directly from the environment, and modeling of experimental melioidosis has been conducted in numerous biologically relevant mo ... | 2010 | 21772830 |
| dna fingerprinting of human isolates of burkholderia pseudomallei from different geographical regions of malaysia. | melioidosis is an infectious disease caused by burkholderia pseudomallei and endemic in southeast asia. one hundred and forty six clinical isolates of b. pseudomallei from different states in malaysia were obtained and molecular typing was carried out using pulsed-field gel electrophoresis (pfge). overall, nine clusters were successfully identified. burkholderia pseudomallei isolates used in this study were found to be genetically diverse and there were differences in the clusters of isolates fr ... | 2010 | 21399594 |
| receptor mimicry as novel therapeutic treatment for biothreat agents. | the specter of intentional release of pathogenic microbes and their toxins is a real threat. this article reviews the literature on adhesins of biothreat agents, their interactions with oligosaccharides and the potential for anti-adhesion compounds as an alternative to conventional therapeutics. the minimal binding structure of ricin has been well characterised and offers the best candidate for successful anti-adhesion therapy based on the galβ1-4glcnac structure. the botulinum toxin serotypes a ... | 2010 | 21327124 |
| development of ceftazidime resistance in burkhoderia pseudomallei in a patient experiencing melioidosis with mediastinal lymphadenitis. | 2010 | 21274496 | |
| "cepacia syndrome" associated with burkholderia cepacia (genomovar i) infection in an adolescent with cystic fibrosis. | 2010 | 21194170 | |
| melioidosis presenting as sepsis syndrome: a case report. | melioidosis is an important disease in asia and australia. it is very rare in venezuela. we describe the case of a 50-year-old diabetic patient with several episodes of right tibial osteomyelitis, left shoulder arthritis, sternal osteomyelitis, right parietal osteomyelitis, and subperiosteal abscess, followed by septic arthritis of the right knee. in all cases gram stain smear showed gram-negative bacilli. culture yielded burkholderia pseudomallei, susceptible to third- and fourth-generation cep ... | 2010 | 21195650 |
| genome-wide analysis reveals loci encoding anti-macrophage factors in the human pathogen burkholderia pseudomallei k96243. | burkholderia pseudomallei is an important human pathogen whose infection biology is still poorly understood. the bacterium is endemic to tropical regions, including south east asia and northern australia, where it causes melioidosis, a serious disease associated with both high mortality and antibiotic resistance. b. pseudomallei is a gram-negative facultative intracellular pathogen that is able to replicate in macrophages. however despite the critical nature of its interaction with macrophages, ... | 2010 | 21203527 |
| inactivation of macrophage rab7 by burkholderia cenocepacia. | strains of the burkholderia cepacia complex can survive within macrophages by arresting the maturation of phagocytic vacuoles. the bacteria preclude fusion of the phagosome with lysosomes by a process that is poorly understood. using murine macrophages, we investigated the stage at which maturation is arrested and analyzed the underlying mechanism. vacuoles containing b. cenocepacia strain j2315, an isolate of the transmissible et12 clone, recruited rab5 and synthesized phosphatidylinositol-3-ph ... | 2010 | 20829607 |
| biodegradation of polyfluorinated biphenyl in bacteria. | fluorinated aromatic compounds are significant environmental pollutants, and microorganisms play important roles in their biodegradation. the effect of fluorine substitution on the transformation of fluorobiphenyl in two bacteria was investigated. pseudomonas pseudoalcaligenes kf707 and burkholderia xenovorans lb400 used 2,3,4,5,6-pentafluorobiphenyl and 4,4'-difluorobiphenyl as sole sources of carbon and energy. the catabolism of the fluorinated compounds was examined by gas chromatography-mass ... | 2010 | 20830605 |
| identification of immunoreactive secretory proteins from the stationary phase culture of burkholderia pseudomallei. | bacterial secreted proteins are known to be involved in virulence and may mediate important host-pathogen interactions. in this study, when the stationary phase culture supernatant of burkholderia pseudomallei was subjected to 2-de, 113 protein spots were detected. fifty-four of the secreted proteins, which included metabolic enzymes, transcription/translation regulators, potential virulence factors, chaperones, transport regulators, and hypothetical proteins, were identified using ms and databa ... | 2010 | 21254130 |
| transport and delivery of antimicrobial agents in burkholderia biofilms. | 2010 | 21529610 | |
| functional quorum sensing systems are maintained during chronic burkholderia cepacia complex infections in patients with cystic fibrosis. | quorum sensing (qs) contributes to the virulence of pseudomonas aeruginosa and burkholderia cepacia complex lung infections. p. aeruginosa qs mutants are frequently isolated from patients with cystic fibrosis. the objective of this study was to determine whether similar adaptations occur over time in b. cepacia complex isolates. forty-five burkholderia multivorans and burkholderia cenocepacia sequential isolates from patients with cystic fibrosis were analyzed for n-acyl-homoserine lactone activ ... | 2010 | 21208930 |
| [in vitro antimicrobial activity of piperacillin-sulbactam and other 7 antimicrobial agents against non-fermentative bacilli]. | to evaluate the in vitro antimicrobial activity of piperacillin-sulbactam against clinical isolates of non-fermentative bacilli isolated from common infections. | 2010 | 21211358 |
| anaerobic crystallization and initial x-ray diffraction data of biphenyl 2,3-dioxygenase from burkholderia xenovorans lb400: addition of agarose improved the quality of the crystals. | biphenyl 2,3-dioxygenase (bpdo; ec 1.14.12.18) catalyzes the initial step in the degradation of biphenyl and some polychlorinated biphenyls (pcbs). bpdolb400, the terminal dioxygenase component from burkholderia xenovorans lb400, a proteobacterial species that degrades a broad range of pcbs, has been crystallized under anaerobic conditions by sitting-drop vapour diffusion. initial crystals obtained using various polyethylene glycols as precipitating agents diffracted to very low resolution (∼8 å ... | 2010 | 21206025 |
| induced biosynthesis of cryptic polyketide metabolites in a burkholderia thailandensis quorum sensing mutant. | genetic manipulation of the luxr-type quorum sensing regulator system in burkholderia thailandensis caused a significant change in the metabolic profile: it led to activation of the thailandamide biosynthesis gene cluster, dramatically increased thailandamide production, and induced strong pigmentation. a novel polyketide metabolite, thailandamide lactone (2), which cannot be detected in the wild type, was isolated from the mutant broth, and its structure was elucidated by high-resolution mass s ... | 2010 | 20853892 |
| colonization of morus alba l. by the plant-growth-promoting and antagonistic bacterium burkholderia cepacia strain lu10-1. | anthracnose, caused by colletotrichum dematium, is a serious threat to the production and quality of mulberry leaves in susceptible varieties. control of the disease has been a major problem in mulberry cultivation. some strains of burkholderia cepacia were reported to be useful antagonists of plant pests and could increase the yields of several crop plants. although b. cepacia lu10-1 is an endophytic bacterium obtained from mulberry leaves, it has not been deployed to control c. dematium infect ... | 2010 | 20854669 |
| pseudomonas quinolone signal affects membrane vesicle production in not only gram-negative but also gram-positive bacteria. | many gram-negative bacteria naturally produce membrane vesicles (mvs) to the extracellular milieu. the pseudomonas quinolone signal (pqs), a quorum-sensing signal of pseudomonas aeruginosa, is a positive regulator of mv production. in this study, we investigated its effects on mv production in other gram-negative and -positive bacterial species. the addition of pqs to an escherichia coli k12 culture resulted in increased mv production and enlarged mvs. an excessive amount of mgcl(2) repressed e. ... | 2010 | 21576862 |
| isolation and characterization of a 4-nitrotoluene-oxidizing enzyme from activated sludge by a metagenomic approach. | to isolate a biocatalytic enzyme, metagenomic libraries were constructed in fosmids from samples of activated sludge used to treat coke plant wastewater. six indigo-producing clones were isolated from approximately 40,000 metagenomic clones in the search for the oxygenase responsible. in vitro mutagenesis and whole-sequencing revealed one open reading frame to be responsible for the production of indigo in the fosmid clones. the deduced sequence of the gene product showed 60% identity with 2-nap ... | 2010 | 21576864 |
| first isolation of burkholderia tropica from a neonatal patient successfully treated with imipenem. | we report the first case of a human burkholderia tropica infection. the patient was a premature neonate who had necrotizing enterocolitis with bowel perforation requiring surgical intervention. the stoma care and difficulties in feeding were a chronic problem. at the age of almost 4 months he developed septicemia due to b. tropica. three consecutive blood cultures grew this organism. the organism was cleared from the blood after a course of imipenem and resolution of post-operative ileus. our ca ... | 2010 | 19482535 |
| prevalence of burkholderia pseudomallei in guangxi, china. | melioidosis, an infectious disease caused by the gram-negative bacterium burkholderia pseudomallei, is now recognized as an important public health problem in southeast asia and tropical northern australia. although b. pseudomallei has been detected in various water and soil samples in southeast china, the enviromental distribution of b. pseudomallei in china is unclear. in the winter months of 2007, 154 and 130 soil and water samples, respectively, were collected from several locations in guang ... | 2010 | 19538822 |
| response of an algal consortium to diesel under varying culture conditions. | a diesel-tolerant sessile freshwater algal consortium obtained from the vicinity of powai lake (mumbai, india) was cultured in the laboratory. the presence of diesel in batch cultures enhanced the maximum specific growth rate of the algal consortium. with decrease in light-dark (l:d) cycle from 20:4 to 4:20 h, the chlorophyll-a levels decreased; however, the removal of diesel was found to be maximum at l:d of 18:6 h with 37.6% degradation over and above controls. in addition to growth in the for ... | 2010 | 19253006 |
| co-expression of the lipase and foldase of pseudomonas aeruginosa to a functional lipase in escherichia coli. | the lipa gene, a structural gene encoding for protein of molecular mass 48 kda, and lipb gene, encoding for a lipase-specific chaperone with molecular mass of 35 kda, of pseudomonas aeruginosa b2264 were co-expressed in heterologous host escherichia coli bl21 (de3) to obtain in vivo expression of functional lipase. the recombinant lipase was expressed with histidine tag at its n terminus and was purified to homogeneity using nickel affinity chromatography. the amino acid sequence of lipa and lip ... | 2010 | 19629472 |
| environmentally relevant parameters affecting pcb degradation: carbon source- and growth phase-mitigated effects of the expression of the biphenyl pathway and associated genes in burkholderia xenovorans lb400. | the principal means for microbial degradation of polychlorinated biphenyls (pcbs) is through the biphenyl pathway. although molecular aspects of the regulation of the biphenyl pathway have been studied, information on environmental facets such as the effect of alternative carbon sources on (polychlorinated) biphenyl degradation is limited. here we explore the effect of environmental conditions (e.g., carbon source and growth phase) on the variation in pcb degradation profiles of burkholderia xen ... | 2010 | 19672561 |
| effects of repeated glyphosate applications on soil microbial community composition and the mineralization of glyphosate. | repeated applications may have a greater impact on the soil microbial community than a single application of glyphosate. experiments were conducted to study the effect of one, two, three, four or five applications of glyphosate on soil microbial community composition and glyphosate mineralization and distribution of (14)c residues in soil. | 2010 | 19697445 |
| burkholderia heleia sp. nov., a nitrogen-fixing bacterium isolated from an aquatic plant, eleocharis dulcis, that grows in highly acidic swamps in actual acid sulfate soil areas of vietnam. | nitrogen-fixing bacteria, strains sa41(t), sa42 and sa53, were isolated from an aquatic plant, eleocharis dulcis, that grows in highly acidic swamps (ph 2-4) in actual acid sulfate soil areas of vietnam. the isolates were gram-negative, aerobic, non-spore-forming, rod-shaped bacteria, having a cell width of 0.6-0.7 microm and a length of 1.5-1.7 microm. they showed good growth between ph 3.0 and 7.0, and between 17 and 37 degrees c. the organisms contained ubiquinone q-8 as the predominant isopr ... | 2010 | 19666801 |
| phase variation has a role in burkholderia ambifaria niche adaptation. | members of the burkholderia cepacia complex (bcc), such as b. ambifaria, are effective biocontrol strains, for instance, as plant growth-promoting bacteria; however, bcc isolates can also cause severe respiratory infections in people suffering from cystic fibrosis (cf). no distinction is known between isolates from environmental and human origins, suggesting that the natural environment is a potential source of infectious bcc species. while investigating the presence and role of phase variation ... | 2010 | 19710710 |
| epidemiology and molecular characterization of a clone of burkholderia cenocepacia responsible for nosocomial pulmonary tract infections in a french intensive care unit. | clustered cases of nosocomial pulmonary infections were observed in a french intensive care unit. biochemical tests showed the etiologic agents to be part of the bcc (bcc). reca polymerase chain reaction-restriction fragment length polymorphism analysis and molecular phylogeny positioned the isolates into burkholderia cenocepacia. their reca sequences were found identical to those of et12 strains responsible of necrotic pneumonia in cystic fibrosis patients. analyses of a multi locus sequence ty ... | 2010 | 19716254 |
| effect of two types of biosurfactants on phenanthrene availability to the bacterial bioreporter burkholderia sartisoli strain rp037. | biosurfactants are tensio-active agents that have often been proposed as a means to enhance the aqueous solubility of hydrophobic organic contaminants, such as polycyclic aromatic hydrocarbons (pahs). biosurfactant-producing bacteria such as those belonging to the genus pseudomonas might therefore enhance pah availability to pah-degrading bacteria. we tested the effects of two types of biosurfactants produced by pseudomonas sp., cyclic lipopeptides and rhamnolipids, on phenanthrene bioavailabili ... | 2010 | 19730847 |
| identification of burkholderia cepacia complex bacteria with a lipopolysaccharide-specific monoclonal antibody. | the genus burkholderia includes many bacteria that cause serious human infections. as is the case with other gram-negative bacteria, burkholderia species produce lps, which is an abundant component of the bacterial cell surface. burkholderia cepacia complex (bcc) bacteria (which include at least 17 separate species) produce lps structures that are quite different. in an attempt to determine the degree of lps epitope variation among bcc species, a mab was produced, designated 5d8, specific for th ... | 2010 | 19729457 |
| structural basis of the affinity for oligomannosides and analogs displayed by bc2l-a, a burkholderia cenocepacia soluble lectin. | the opportunistic pathogen burkholderia cenocepacia contains three soluble carbohydrate-binding proteins, related to the fucose-binding lectin pa-iil from pseudomonas aeruginosa. all contain a pa-iil-like domain and two of them have an additional n-terminal domain that displays no sequence similarities with known proteins. printed arrays screening performed on the shortest one, b. cenocepacia lectin a (bc2l-a), demonstrated the strict specificity for oligomannose-type n-glycan structures (lameig ... | 2010 | 19770128 |
| [arthritis caused by burkholderia cepacia]. | 2010 | 19783076 | |
| in vitro pharmacodynamics of levofloxacin and other aerosolized antibiotics under multiple conditions relevant to chronic pulmonary infection in cystic fibrosis. | the inhalational administration of antibiotics can provide high concentrations locally in the lungs of cystic fibrosis patients and, thus, can be useful for the treatment of chronic bacterial infections. the present study evaluated the in vitro activities of levofloxacin, ciprofloxacin, tobramycin, amikacin, and aztreonam against clinical isolates of pseudomonas aeruginosa, burkholderia cepacia complex, stenotrophomonas maltophilia, alcaligenes xylosoxidans, and staphylococcus aureus from cystic ... | 2010 | 19805554 |
| burkholderia acidipaludis sp. nov., aluminum-tolerant bacteria isolated from chinese water chestnut (eleocharis dulcis) growing in highly acidic swamps in south-east asia. | two strains of aluminium-tolerant bacteria, sa33(t) and 7a078, were isolated from chinese water chestnut (eleocharis dulcis) growing in highly acidic swamps (ph 2-4) in actual acid sulfate soil areas of vietnam (sa33(t)) and thailand (7a078). the strains were gram-negative, aerobic, non-spore-forming rods, 0.6-0.7 mum wide and 1.3-1.7 mum long. these strains showed good growth at ph 3.0-8.0 and 17-37 degrees c. the organisms contained ubiquinone q-8 as the predominant isoprenoid quinone and c(16 ... | 2010 | 19819996 |
| identifying translation initiation sites in prokaryotes using support vector machine. | motivation: gene identification in genomes has been a fundamental and long-standing task in bioinformatics and computational biology. many computational methods have been developed to predict genes in prokaryote genomes by identifying translation initiation site (tis) in transcript data. however, the pseudo-tiss at the genome level make these methods suffer from a high number of false positive predictions. in addition, most of the existing tools use an unsupervised learning framework, whose pred ... | 2010 | 19840808 |
| pathema: a clade-specific bioinformatics resource center for pathogen research. | pathema (http://pathema.jcvi.org) is one of the eight bioinformatics resource centers (brcs) funded by the national institute of allergy and infectious disease (niaid) designed to serve as a core resource for the bio-defense and infectious disease research community. pathema strives to support basic research and accelerate scientific progress for understanding, detecting, diagnosing and treating an established set of six target niaid category a-c pathogens: category a priority pathogens; bacillu ... | 2010 | 19843611 |
| the clinical profile and predictors of mortality in patients with melioidosis in south india. | melioidosis is an underdiagnosed and underreported disease in india with protean clinical manifestations. mortality in this study population was 17%. the predominant risk factor for melioidosis was diabetes mellitus. multifocal disease was present in 66% and pulmonary involvement in 61% of patients. in a country like india where the conditions are conducive for endemic melioidosis and due to the clinical similarity of melioidosis to diseases like tuberculosis, it is essential for clinicians to h ... | 2010 | 19850605 |
| differential susceptibility of inbred mouse strains to burkholderia thailandensis aerosol infection. | burkholderia pseudomallei is the causative agent of melioidosis, an emerging bacterial disease that accounts for high rates of septicaemia and death in parts of southeast asia and northern australia. the closely related species burkholderia thailandensis is considered avirulent in humans and has been used as a surrogate for b. pseudomallei in several studies. the pathogenesis of b. pseudomallei and the role of toll-like receptors (tlrs) in host immunity to infection are not well-defined. in this ... | 2010 | 19853031 |
| icmf is a fusion between the radical b12 enzyme isobutyryl-coa mutase and its g-protein chaperone. | coenzyme b(12) is used by two highly similar radical enzymes, which catalyze carbon skeleton rearrangements, methylmalonyl-coa mutase and isobutyryl-coa mutase (icm). icm catalyzes the reversible interconversion of isobutyryl-coa and n-butyryl-coa and exists as a heterotetramer. in this study, we have identified >70 bacterial proteins, which represent fusions between the subunits of icm and a p-loop gtpase and are currently misannotated as methylmalonyl-coa mutases. we designate this fusion prot ... | 2010 | 19864421 |
| the lipid a of burkholderia multivorans c1576 smooth-type lipopolysaccharide and its pro-inflammatory activity in a cystic fibrosis airways model. | cystic fibrosis is an autosomal recessive disorder and it is characterised by chronic bacterial airway infection which leads to progressive lung deterioration, sometimes with fatal outcome. burkholderia multivorans and burkholderia cenocepacia are the species responsible for most of the infections of cystic fibrosis patients. lipopolysaccharide endotoxins (lpss) are among the foremost factors of pathogenesis of gram-negative infection and, in particular, lipid a is the endotoxic portion of lps r ... | 2010 | 19880661 |
| burkholderia mallei cluster 1 type vi secretion mutants exhibit growth and actin polymerization defects in raw 264.7 murine macrophages. | burkholderia mallei is a facultative intracellular pathogen that causes severe disease in animals and humans. recent studies have shown that the cluster 1 type vi secretion system (t6ss-1) expressed by this organism is essential for survival in a hamster model of glanders. to better understand the role of t6ss-1 in the pathogenesis of disease, studies were initiated to examine the interactions of b. mallei tsse mutants with raw 264.7 murine macrophages. results obtained by utilizing modified gen ... | 2010 | 19884331 |
| molecular and biochemical characterization of the natural chromosome-encoded class a beta-lactamase from pseudomonas luteola. | pseudomonas luteola (formerly classified as cdc group ve-1 and named chryseomonas luteola) is an unusual pathogen implicated in rare but serious infections in humans. a novel beta-lactamase gene, bla(lut-1), was cloned from the whole-cell dna of the p. luteola clinical isolate lam, which had a weak narrow-spectrum beta-lactam-resistant phenotype, and expressed in escherichia coli. this gene encoded lut-1, a 296-amino-acid ambler class a beta-lactamase with a pi of 6 and a theoretical molecular m ... | 2010 | 19884377 |
| [a sensitive, specific and predictive isolation medium developed for stenotrophomonas maltophilia study in healthcare settings]. | stenotrophomonas maltophilia (smalto) is a prominent nosocomial pathogen, commonly isolated in the hospital environment. multiple smalto nosocomial outbreaks have been linked to contaminated water sources. this study aimed to develop a medium able to ease healthcare environment smalto isolation. | 2010 | 19892479 |
| mycotic iliac aneurysm associated with burkholderia pseudomallei. | 2010 | 19897393 | |
| colloidal and bacterial deposition: role of gravity. | the role of gravitational force on the deposition of 0.5, 1.1, and 1.8 mum carboxylate-modified polystyrene latex (cml) microspheres and bacterium burkholderia cepacia g4g has been evaluated using a parallel plate flow chamber system. this experimental system utilized an inverted and an upright optical microscope attached with image-capturing devices to directly observe and determine the deposition kinetics onto glass surfaces located at the top and bottom of the flow chamber. deposition kinetic ... | 2010 | 19911823 |
| entry of burkholderia organisms into respiratory epithelium: cftr, microfilament and microtubule dependence. | the pathogenesis of infection with burkholderia cepacia complex (bcc) organisms may be linked to its capacity to invade respiratory epithelium. | 2010 | 19914879 |
| characterization of chlorophenol 4-monooxygenase (tftd) and nadh:fad oxidoreductase (tftc) of burkholderia cepacia ac1100. | burkholderia cepacia ac1100 completely degrades 2,4,5-trichlorophenol, in which an fadh(2)-dependent monooxygenase (tftd) and an nadh:fad oxidoreductase (tftc) catalyze the initial steps. tftd oxidizes 2,4,5-trichlorophenol (2,4,5-tcp) to 2,5-dichloro-p-benzoquinone, which is chemically reduced to 2,5-dichloro-p-hydroquinone (2,5-dichq). then, tftd oxidizes the latter to 5-chloro-2-hydroxy-p-benzoquinone. in those processes, tftc provides all the required fadh(2). we have determined the crystal ... | 2010 | 19915006 |
| diabetes as a determinant of mortality in cystic fibrosis. | diabetes is increasingly common in cystic fibrosis, but little information describing its influence on mortality exists. using national u.k. data, in this study we document diabetes-specific mortality rates, estimate the impact of diabetes on survival, and estimate population-attributable fractions. | 2010 | 19918014 |