Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| phenotypic and genetic changes in the life cycle of small colony variants of salmonella enterica serotype typhimurium induced by streptomycin. | small colony variants (scvs), constituting a slow-growing subpopulation of bacteria that facilitates persistence in lethal environmental conditions, are able to revert to the phenotype of rapid growth for further proliferation and transmission. salmonella enterica serotype typhimurium is one of the most important foodborne pathogens. this study investigated the genetic mechanisms how scvs induced by streptomycin reverted to the fast-growing phenotype and the phenotypic changes of scvs among thei ... | 2016 | 27245674 |
| poor biofilm-forming ability and long-term survival of invasive salmonella typhimurium st313. | salmonella enterica serovar typhimurium, an enteric pathogen that causes a self-limiting gastroenteritis, forms biofilms on different surfaces. in sub-saharan africa, salmonella typhimurium of a novel sequence type (st) 313 was identified and produces septicemia in the absence of gastroenteritis. no animal reservoir has been identified, and it is hypothesized that transmission occurs via human to human. in this study, we show that invasive salmonella typhimurium st313 strains from mali are poor ... | 2016 | 27222487 |
| comparison of methods for quantitating salmonella enterica typhimurium and heidelberg strain attachment to reusable plastic shipping container coupons and preliminary assessment of sanitizer efficacy. | salmonella serovars, one of the leading contributors to foodborne illness and are especially problematic for foods that are not cooked before consumption, such as fresh produce. the shipping containers that are used to transport and store fresh produce may play a role in cross contamination and subsequent illnesses. however, methods for quantitatively attached cells are somewhat variable. the overall goal of this study was to compare conventional plating with molecular methods for quantitating a ... | 2016 | 27192211 |
| strains, mechanism, and perspective: salmonella-based cancer therapy. | recently, investigation of bacterial-based tumor therapy has regained focus due to progress in molecular, cellular, and microbial biology. many bacteria such as salmonella, listeria, escherichia, and clostridium have proved to have tumor targeting and in some cases even tumor-destroying phenotypes. furthermore, bacterial clinical treatments for cancer have been improved by combination with other therapeutic methods such as chemotherapeutic drugs and radioactive agents. synthetic biology techniqu ... | 2016 | 27190519 |
| pediatric epidemic of salmonella enterica serovar typhimurium in the area of l'aquila, italy, four years after a catastrophic earthquake. | a salmonella enterica epidemic occurred in children of the area of l'aquila (central italy, abruzzo region) between june 2013 and october 2014, four years after the catastrophic earthquake of 6 april 2009. | 2016 | 27164121 |
| rapid dna transformation in salmonella typhimurium by the hydrogel exposure method. | even with advances in molecular cloning and dna transformation, new or alternative methods that permit dna penetration in salmonella enterica subspecies enterica serovar typhimurium are required in order to use this pathogen in biotechnological or medical applications. in this work, an adapted protocol of bacterial transformation with plasmid dna based on the "yoshida effect" was applied and optimized on salmonella enterica serovar typhimurium lt2 reference strain. the plasmid transference based ... | 2016 | 27154729 |
| bacteriophages with potential to inactivate salmonella typhimurium: use of single phage suspensions and phage cocktails. | the aim of this study was to compare the dynamics of three previously isolated bacteriophages (or phages) individually (phse-1, phse-2 and phse-5) or combined in cocktails of two or three phages (phse-1/phse-2, phse-1/phse-5, phse-2/phse-5 and phse-1/phse-2/phse-5) to control salmonella enterica serovar typhimurium (salmonella typhimurium) in order to evaluate their potential application during depuration. phages were assigned to the family siphoviridae and revealed identical restriction digest ... | 2016 | 27126773 |
| outbreak of unusual salmonella enterica serovar typhimurium monophasic variant 1,4 [5],12:i:-, italy, june 2013 to september 2014. | monophasic variant of salmonella enterica subspecies enterica serovar typhimurium (monophasic s. typhimurium), with antigenic structure 1,4,[5],12:i:-, appears to be of increasing importance in europe. in italy, monophasic s. typhimurium represented the third most frequent salmonella serovar isolated from human cases between 2004 and 2008. from june 2013 to october 2014, a total of 206 human cases of salmonellosis were identified in abruzzo region (central italy). obtained clinical isolates char ... | 2016 | 27105170 |
| assessing the effect of sodium dichloroisocyanurate concentration on transfer of salmonella enterica serotype typhimurium in wash water for production of minimally processed iceberg lettuce (lactuca sativa l.). | this study evaluated the impact of sodium dichloroisocyanurate (5, 10, 20, 30, 40, 50 and 250 mg l(-1) ) in wash water on transfer of salmonella typhimurium from contaminated lettuce to wash water and then to other noncontaminated lettuces washed sequentially in the same water. experiments were designed mimicking the conditions commonly seen in minimally processed vegetable (mpv) processing plants in brazil. the scenarios were as follows: (1) washing one inoculated lettuce portion in nonchlorina ... | 2016 | 27105015 |
| complete genome sequence of salmonella enterica serovar typhimurium strain so3 (sequence type 302) isolated from a baby with meningitis in mexico. | the complete genome of italic! salmonella entericaserovar typhimurium strain so3 (sequence type 302), isolated from a fatal meningitis infection in mexico, was determined using pacbio technology. the chromosome hosts six complete prophages and is predicted to harbor 51 genomic islands, including 13 pathogenicity islands (spis). it carries the italic! salmonellavirulence plasmid (pstv). | 2016 | 27103717 |
| complete genome sequence of salmonella enterica serovar typhimurium strain yu15 (sequence type 19) harboring the salmonella genomic island 1 and virulence plasmid pstv. | the complete genome ofsalmonella entericasubsp.entericaserovar typhimurium sequence type 19 (st19) strain yu15, isolated in yucatán, mexico, from a human baby stool culture, was determined using pacbio technology. the chromosome contains five intact prophages and thesalmonellagenomic island 1 (sgi1). this strain carries thesalmonellavirulence plasmid pstv. | 2016 | 27081132 |
| depletion of butyrate-producing clostridia from the gut microbiota drives an aerobic luminal expansion of salmonella. | the mammalian intestine is host to a microbial community that prevents pathogen expansion through unknown mechanisms, while antibiotic treatment can increase susceptibility to enteric pathogens. here we show that streptomycin treatment depleted commensal, butyrate-producing clostridia from the mouse intestinal lumen, leading to decreased butyrate levels, increased epithelial oxygenation, and aerobic expansion of salmonella enterica serovar typhimurium. epithelial hypoxia and salmonella restricti ... | 2016 | 27078066 |
| selection and characterization of aptamers using a modified whole cell bacterium selex for the detection of salmonella enterica serovar typhimurium. | this study describes the selection of single-stranded dna (ssdna) aptamers against salmonella enterica serovar typhimurium using a modified whole cell systematic evolution of ligands by exponential enrichment (whole cell selex). for evolving specific aptamers, ten rounds of selection to live salmonella cells, alternating with negative selection against a cocktail of related pathogens, were performed. the resulting highly enriched oligonucleotide pools were sequenced and clustered into eight grou ... | 2016 | 27070414 |
| ctx-m-27 producing salmonella enterica serotypes typhimurium and indiana are prevalent among food-producing animals in china. | salmonella spp. is one of the most important food-borne pathogens causing digestive tract and invasive infections in both humans and animals. extended-spectrum β-lactamases (esbls) especially the ctx-m-type esbls are increasingly being reported worldwide and in china. these studies seldom focused on salmonella isolates from food-producing animals. the aim of this study was to characterize the antimicrobial resistance profiles, serotypes and esbls and in particular, ctx-m producing salmonella iso ... | 2016 | 27065989 |
| impacts of salmonella enterica serovar typhimurium and its speg gene on the transcriptomes of in vitro m cells and caco-2 cells. | microfold or membranous (m) cells are specialized intestinal epithelial cells responsible for host immunity. the speg mutant of salmonella typhimurium (s. typhimurium) is a nonreplicating strain within human cells to be a candidate vaccine vector for interacting with m cells. we conducted this study to identify the genes are differently expressed between in vitro m cells and caco-2 cells, and to determine whether s. typhimurium and speg affect the transcriptomes of both cell types. in vitro m ce ... | 2016 | 27064787 |
| multiple roles of putrescine and spermidine in stress resistance and virulence of salmonella enterica serovar typhimurium. | polyamines (putrescine and spermidine) are small-cationic amines ubiquitous in nature and present in most living cells. in recent years they have been linked to virulence of several human pathogens including shigella spp and salmonella enterica serovar typhimurium (s. typhimurium). central to s. typhimurium virulence is the ability to survive and replicate inside macrophages and resisting the antimicrobial attacks in the form of oxidative and nitrosative stress elicited from these cells. in the ... | 2016 | 27041598 |
| salmonella typhimurium infection primes a nutriprive mechanism in piglets. | salmonella enterica serovar typhimurium (s. typhimurium) is an important cause of acute food- borne zoonoses worldwide, typically carried by pigs. it is well known that salmonella has evolved a wide array of strategies enabling it to invade the host, but little information is available on the specific host responses to salmonella infections. in the present study, we used an in vivo approach (involving piglets infected with a virulent or an attenuated s. typhimurium strain) coupled to histologica ... | 2016 | 27016766 |
| immunogenicity and cross-protective efficacy induced by outer membrane proteins from salmonella typhimurium mutants with truncated lps in mice. | lipopolysaccharide (lps) is a major virulence factor present in the outer membrane of salmonella enterica serovar typhimurium (s. typhimurium). outer membrane proteins (omps) from salmonella show high immunogenicity and provide protection against salmonella infection, and truncated lps alters the outer membrane composition of the cell wall. in our previous study, we demonstrated that salmonella mutants carrying truncated lps failed to induce strong immune responses and cross-reaction to other en ... | 2016 | 27011167 |
| antioxidant defense by thioredoxin can occur independently of canonical thiol-disulfide oxidoreductase enzymatic activity. | the thiol-disulfide oxidoreductase cxxc catalytic domain of thioredoxin contributes to antioxidant defense in phylogenetically diverse organisms. we find that although the oxidoreductase activity of thioredoxin-1 protects salmonella enterica serovar typhimurium from hydrogen peroxide in vitro, it does not appear to contribute to salmonella's antioxidant defenses in vivo. nonetheless, thioredoxin-1 defends salmonella from oxidative stress resulting from nadph phagocyte oxidase macrophage expressi ... | 2016 | 26997275 |
| increased efficacy of inactivated vaccine candidates prepared with salmonella enterica serovar typhimurium strains of predominant genotypes in ducks. | salmonella enterica serovar typhimurium has been a major causative agent of food-borne human disease, mainly due to consumption of contaminated food animal products. in particular, ducks serve as a reservoir of serovar typhimurium, and are one of the common sources of human infection. to prevent infection of ducks, and therefore minimize human infection, it is critical to control the persistent epidemic strains in ducks. here, we analyzed the genetic diversity and virulence of serovar typhimuriu ... | 2016 | 26994209 |
| specific monoclonal antibody overcomes the salmonella enterica serovar typhimurium's adaptive mechanisms of intramacrophage survival and replication. | salmonella-specific antibodies play an important role in host immunity; however, the mechanisms of salmonella clearance by pathogen-specific antibodies remain to be completely elucidated since previous studies on antibody-mediated protection have yielded inconsistent results. these inconsistencies are at least partially attributable to the use of polyclonal antibodies against salmonella antigens. here, we developed a new monoclonal antibody (mab)-449 and identified its related immunogen that pro ... | 2016 | 26986057 |
| proteomic insight into the effects of the salmonella ubiquitin ligase slrp on host cells. | the virulence of the human and animal pathogen salmonella enterica serovar typhimurium is dependent on two type iii secretion systems. these systems translocate proteins called effectors into eukaryotic host cells. slrp is a salmonella type iii secretion effector with ubiquitin ligase activity. here, we used two complementary proteomic approaches, two-dimensional gel electrophoresis and itraq (isobaric tags for relative and absolute quantification) to study the consequences of the presence of sl ... | 2016 | 26966069 |
| global genomic epidemiology of salmonella enterica serovar typhimurium dt104. | it has been 30 years since the initial emergence and subsequent rapid global spread of multidrug-resistant salmonella entericaserovar typhimurium dt104 (mdr dt104). nonetheless, its origin and transmission route have never been revealed. we used whole-genome sequencing (wgs) and temporally structured sequence analysis within a bayesian framework to reconstruct temporal and spatial phylogenetic trees and estimate the rates of mutation and divergence times of 315s typhimurium dt104 isolates sample ... | 2016 | 26944846 |
| characterization of salmonella enterica serovar typhimurium isolates from pigs and pig environment-related sources and evidence of new circulating monophasic strains in spain. | a total of 117 salmonella enterica serovar typhimurium and 59 monophasic salmonella typhimurium (s. enterica serovar 4,[5],12:i:-) strains isolated between 2008 and 2012 from pig, wild bird, rodent, and farm environment samples from the northeast of spain were characterized by phage typing, antibiotic susceptibility testing, and multiple-locus variable-number tandem repeat analysis in order to evaluate their phenotypic and genetic relatedness. in salmonella 4,[5],12:i:-, the most prevalent phage ... | 2016 | 26939650 |
| optimizing salmonella enterica serovar typhimurium for bacteria-mediated tumor therapy. | bacteria-mediated tumor therapy using salmonella enterica serovar typhimurium is a therapeutic option with great potential. numerous studies explored the potential of salmonella typhimurium for therapeutic applications, however reconciling safety with vectorial efficacy remains a major issue. recently we have described a conditionally attenuated salmonella vector that is based on genetic lipopolysaccharide modification. this vector combines strong attenuation with appropriate anti-tumor properti ... | 2016 | 26939530 |
| detection of a novel gyrb mutation associated with fluoroquinolone-nonsusceptible salmonella enterica serovar typhimurium isolated from a bloodstream infection in ghana. | a multidrug-resistant salmonella enterica serovar typhimurium with reduced susceptibility to ciprofloxacin was isolated from the blood of a hospitalized child in ghana. dna sequencing identified a novel gyrb mutation at codon 466 (glu466asp). an increase in fluoroquinolone susceptibility after the introduction of a wild-type gyrb(+) allele demonstrated that the gyrb466 mutation had a direct effect on fluoroquinolone susceptibility. | 2016 | 26933021 |
| determination of the stoichiometry of the complete bacterial type iii secretion needle complex using a combined quantitative proteomic approach. | precisely knowing the stoichiometry of their components is critical for investigating structure, assembly, and function of macromolecular machines. this has remained a technical challenge in particular for large, hydrophobic membrane-spanning protein complexes. here, we determined the stoichiometry of a type iii secretion system of salmonella enterica serovar typhimurium using two complementary protocols of gentle complex purification combined with peptide concatenated standard and synthetic sta ... | 2016 | 26900162 |
| intestinal long-chain fatty acids act as a direct signal to modulate expression of the salmonella pathogenicity island 1 type iii secretion system. | salmonella enterica serovar typhimurium uses the salmonella pathogenicity island 1 (spi1) type iii secretion system (t3ss) to induce inflammatory diarrhea and bacterial uptake into intestinal epithelial cells. the expression of hila, encoding the transcriptional activator of the t3ss structural genes, is directly controlled by three arac-like regulators, hild, hilc, and rtsa, each of which can activate hild, hilc, rtsa, and hila genes, forming a complex feed-forward regulatory loop. expression o ... | 2016 | 26884427 |
| outer membrane vesicle production facilitates lps remodeling and outer membrane maintenance in salmonella during environmental transitions. | the ability of gram-negative bacteria to carefully modulate outer membrane (om) composition is essential to their survival. however, the asymmetric and heterogeneous structure of the gram-negative om poses unique challenges to the cell's successful adaption to rapid environmental transitions. although mechanisms to recycle and degrade om phospholipid material exist, there is no known mechanism by which to remove unfavorable lipopolysaccharide (lps) glycoforms, except slow dilution through cell g ... | 2016 | 27795394 |
| stabilization of the virulence plasmid pslt of salmonella typhimurium by three maintenance systems and its evaluation by using a new stability test. | certain salmonella enterica serovars belonging to subspecies i carry low-copy-number virulence plasmids of variable size (50-90 kb). all of these plasmids share the spv operon, which is important for systemic infection. virulence plasmids are present at low copy numbers. few copies reduce metabolic burden but suppose a risk of plasmid loss during bacterial division. this drawback is counterbalanced by maintenance modules that ensure plasmid stability, including partition systems and toxin-antito ... | 2016 | 27800482 |
| the function of ile-x-ile motif in the oligomerization and chaperone-like activity of small heat shock protein agsa at room temperature. | small heat shock proteins assemble as large oligomers in vitro and exhibit atp-independent chaperone activities. ile-x-ile motif is essential in both the function and oligomer formation. agsa of salmonella enterica serovar typhimurium has been demonstrated to adopt large oligomeric structure and possess strong chaperone activity. size exclusion chromatography, non-denaturing pore gradient page, and negatively stain electron microscopic analysis of the various c-terminal truncated mutants were pe ... | 2016 | 27812886 |
| biosynthesis of selenate reductase in salmonella enterica: critical roles for the signal peptide and dmsd. | salmonella enterica serovar typhimurium is a gram-negative bacterium with a flexible respiratory capability. under anaerobic conditions, s. enterica can utilize a range of terminal electron acceptors, including selenate, to sustain respiratory electron transport. the s. enterica selenate reductase is a membrane-bound enzyme encoded by the ynfefgh-dmsd operon. the active enzyme is predicted to comprise at least three subunits where ynfe is a molybdenum-containing catalytic subunit. the ynfe prote ... | 2016 | 27902441 |
| protective immunity elicited by oral immunization of mice with salmonella enterica serovar typhimurium braun lipoprotein (lpp) and acetyltransferase (msbb) mutants. | we evaluated the extent of attenuation and immunogenicity of the δlppab and δlppab δmsbb mutants of salmonella enterica serovar typhimurium when delivered to mice by the oral route. these mutants were deleted either for the braun lipoprotein genes (lppa and lppb) or in combination with the msbb gene, which encodes an acetyltransferase required for lipid a modification of lipopolysaccharide. both the mutants were attenuated (100% animal survival) and triggered robust innate and adaptive immune re ... | 2016 | 27891321 |
| studies on the therapeutic effect of propolis along with standard antibacterial drug in salmonella enterica serovar typhimurium infected balb/c mice. | antibiotic resistance is an emerging public health problem. centers for disease control and prevention (cdc) has described antibiotic resistance as one of the world's most pressing health problems in 21(st) century. who rated antibiotic resistance as "one of the three greatest threats to human health". one important strategy employed to overcome this resistance is the use of combination of drugs. many plants, natural extracts have been shown to exhibit synergistic response with standard drugs ag ... | 2016 | 27887651 |
| genome-guided design of a defined mouse microbiota that confers colonization resistance against salmonella enterica serovar typhimurium. | protection against enteric infections, also termed colonization resistance, results from mutualistic interactions of the host and its indigenous microbes. the gut microbiota of humans and mice is highly diverse and it is therefore challenging to assign specific properties to its individual members. here, we have used a collection of murine bacterial strains and a modular design approach to create a minimal bacterial community that, once established in germ-free mice, provided colonization resist ... | 2016 | 27869789 |
| mg2+ regulates transcription of mgta in salmonella typhimurium via translation of proline codons during synthesis of the mgtl peptide. | in salmonella enterica serovar typhimurium, mg(2+) limitation induces transcription of the mgta mg(2+) transport gene, but the mechanism involved is unclear. the 5' leader of the mgta mrna contains a 17-codon, proline-rich orf, mgtl, whose translation regulates the transcription of mgta [park s-y et al. (2010) cell 142:737-748]. rapid translation of mgtl promotes formation of a secondary structure in the mgta mrna that permits termination of transcription by the rho protein upstream of mgta, whe ... | 2016 | 27849575 |
| single-cell rna-seq ties macrophage polarization to growth rate of intracellular salmonella. | intracellular bacterial pathogens can exhibit large heterogeneity in growth rate inside host cells, with major consequences for the infection outcome. if and how the host responds to this heterogeneity remains poorly understood. here, we combined a fluorescent reporter of bacterial cell division with single-cell rna-sequencing analysis to study the macrophage response to different intracellular states of the model pathogen salmonella enterica serovar typhimurium. the transcriptomes of individual ... | 2016 | 27841856 |
| effect of holarrhena antidysentrica (ha) and andrographis paniculata (ap) on the biofilm formation and cell membrane integrity of opportunistic pathogen salmonella typhimurium. | increasing occurrence of gastroenteritis outbreaks caused by food borne opportunistic microorganisms has become a major problem in food industry as well as in immunocompromised host. antimicrobial agents are losing their efficacy due to increase in the microbial resistance. for such reasons, conventional treatment has become limited to manage the infections state. need of the hour is to instigate the search for safer holistic alternatives. the present study was hence conducted to assess the anti ... | 2016 | 27836761 |
| a proteomic approach to the development of diva elisa distinguishing pigs infected with salmonella typhimurium and pigs vaccinated with a salmonella typhimurium-based inactivated vaccine. | salmonella enterica serovar typhimurium is one of the most common enteropathogenic bacteria found in pigs in europe. in our previous work, we demonstrated the protective effects in suckling piglets when their dams had been vaccinated with an s. typhimurium-based inactivated vaccine. this study is focused on a procedure leading to serological discrimination between vaccinated and infected pigs. as we supposed, distinct environment during natural infection and in bacterial cultures used for vaccin ... | 2016 | 27835998 |
| pgadb-builder: a web service tool for creating pan-genome allele database for molecular fine typing. | with the advance of next generation sequencing techniques, whole genome sequencing (wgs) is expected to become the optimal method for molecular subtyping of bacterial isolates. to use wgs as a general subtyping method for disease outbreak investigation and surveillance, the layout of wgs-based typing must be comparable among laboratories. whole genome multilocus sequence typing (wgmlst) is an approach that achieves this requirement. to apply wgmlst as a standard subtyping approach, a pan-genome ... | 2016 | 27824078 |
| the infectious intracellular lifestyle of salmonella enterica relies on the adaptation to nutritional conditions within the salmonella-containing vacuole. | salmonella enterica serovar typhimurium (s. typhimurium) is a gram-negative pathogen that causes various host-specific diseases. during their life cycle, salmonellae survive frequent exposures to a variety of environmental stresses, e.g. carbon-source starvation. the virulence of this pathogen relies on its ability to establish a replicative niche, named salmonella-containing vacuole, inside host cells. however, the microenvironment of the scv and the bacterial metabolic pathways required during ... | 2016 | 27936347 |
| peroxiredoxin-3 is involved in bactericidal activity through the regulation of mitochondrial reactive oxygen species. | peroxiredoxin-3 (prdx3) is a mitochondrial protein of the thioredoxin family of antioxidant peroxidases and is the principal peroxidase responsible for metabolizing mitochondrial hydrogen peroxide. recent reports have shown that mitochondrial reactive oxygen species (mros) contribute to macrophage-mediated bactericidal activity in response to toll-like receptors. herein, we investigated the functional effect of prdx3 in bactericidal activity. the mitochondrial localization of prdx3 in hek293t ce ... | 2016 | 28035213 |
| near-atomic-resolution cryo-em analysis of the salmonella t3s injectisome basal body. | the type iii secretion (t3s) injectisome is a specialized protein nanomachine that is critical for the pathogenicity of many gram-negative bacteria, including purveyors of plague, typhoid fever, whooping cough, sexually transmitted infections and major nosocomial infections. this syringe-shaped 3.5-mda macromolecular assembly spans both bacterial membranes and that of the infected host cell. the internal channel formed by the injectisome allows for the direct delivery of partially unfolded virul ... | 2016 | 27974800 |
| an unusual case of septic arthritis of the hip. | non-typhoidal salmonella, particularly salmonella enterica serovar typhimurium is food borne pathogen causing mild self-limiting diarrhoea in healthy adults. it can occasionally cause extraintestinal focal infection in susceptible patients. salmonella, as the aetiological agent of osteomyelitis and septic arthritis is rare and has been mostly reported in patients with sickle cell disease or thalassaemia. we report a case of septic arthritis by salmonellatyphimurium in an immunocompromised patien ... | 2016 | 28050370 |
| real-time optotracing of curli and cellulose in live salmonella biofilms using luminescent oligothiophenes. | extracellular matrix (ecm) is the protein- and polysaccharide-rich backbone of bacterial biofilms that provides a defensive barrier in clinical, environmental and industrial settings. understanding the dynamics of biofilm formation in native environments has been hindered by a lack of research tools. here we report a method for simultaneous, real-time, in situ detection and differentiation of the salmonella ecm components curli and cellulose, using non-toxic, luminescent conjugated oligothiophen ... | 2016 | 28721253 |
| evolution of variable number tandem repeats and its relationship with genomic diversity in salmonella typhimurium. | salmonella enterica serovar typhimurium is the most common salmonella serovar causing human infections in australia and many other countries. a total of 12,112 s. typhimurium isolates from new south wales were analyzed by multi-locus variable number of tandem repeat (vntr) analysis (mlva) using five vntrs from 2007 to 2014. we found that mid ranges of repeat units of 8-14 in vntr locus sttr5, 6-13 in sttr6, and 9-12 in sttr10 were always predominant in the population (>50%). in vitro passaging e ... | 2016 | 28082952 |
| physiological fluid shear alters the virulence potential of invasive multidrug-resistant non-typhoidal salmonella typhimurium d23580. | salmonella enterica serovar typhimurium strains belonging to sequence type st313 are a major cause of fatal bacteremia among hiv-infected adults and children in sub-saharan africa. unlike "classical" non-typhoidal salmonella (nts), gastroenteritis is often absent during st313 infections and isolates are most commonly recovered from blood, rather than from stool. this is consistent with observations in animals, in which st313 strains displayed lower levels of intestinal colonization and higher re ... | 2016 | 28725732 |
| towards an understanding of salmonella enterica serovar typhimurium persistence in swine. | salmonella enterica is an important food borne pathogen that is frequently carried by swine. carrier animals pose a food safety risk because they can transmit s. enterica to finished food products in the processing plant or by contamination of the environment. environmental contamination has become increasingly important as non-animal foods (plant-based) have been implicated as sources of s. enterica. the prevalence of s. enterica in swine is high and yet carrier animals remain healthy. s. enter ... | 2016 | 28155802 |
| exploiting a host-commensal interaction to promote intestinal barrier function and enteric pathogen tolerance. | commensal intestinal bacteria can prevent pathogenic infection; however, limited knowledge of the mechanisms by which individual bacterial species contribute to pathogen resistance has restricted their potential for therapeutic application. here, we examined how colonization of mice with a human commensal enterococcus faecium protects against enteric infections. we show that e. faecium improves host intestinal epithelial defense programs to limit salmonella enterica serotype typhimurium pathogen ... | 2016 | 28580440 |
| loss of multicellular behavior in epidemic african nontyphoidal salmonella enterica serovar typhimurium st313 strain d23580. | nontyphoidal salmonella enterica serovar typhimurium is a frequent cause of bloodstream infections in children and hiv-infected adults in sub-saharan africa. most isolates from african patients with bacteremia belong to a single sequence type, st313, which is genetically distinct from gastroenteritis-associated st19 strains, such as 14028s and sl1344. some studies suggest that the rapid spread of st313 across sub-saharan africa has been facilitated by anthroponotic (person-to-person) transmissio ... | 2016 | 26933058 |
| live attenuated human salmonella vaccine candidates: tracking the pathogen in natural infection and stimulation of host immunity. | salmonellosis, caused by members of the genus salmonella, is responsible for considerable global morbidity and mortality in both animals and humans. in this review, we will discuss the pathogenesis of salmonella enterica serovar typhi and salmonella enterica serovar typhimurium, focusing on human salmonella infections. we will trace the path of salmonella through the body, including host entry sites, tissues and organs affected, and mechanisms involved in both pathogenesis and stimulation of hos ... | 2016 | 27809955 |
| lose to win: mart pseudogenization in salmonella enterica serovar typhi contributed to the surv-dependent survival to h2o2, and inside human macrophage-like cells. | the difference in host range between salmonella enterica serovar typhimurium (s. typhimurium) and salmonella enterica serovar typhi (s. typhi) can be partially attributed to the gain of functions, to the loss of functions (i.e. pseudogenization), or to a combination of both processes. as previously reported, the loss of functions by pseudogenization may play a role in bacterial evolution, especially in host-restricted pathogens such as s. typhi. the mart-fidl operon, located at the spi-3, encode ... | 2016 | 27567490 |
| salmonella extracellular matrix components influence biofilm formation and gallbladder colonization. | salmonella enterica serovar typhi, the causative agent of typhoid fever in humans, forms biofilms encapsulated by an extracellular matrix (ecm). biofilms facilitate colonization and persistent infection in gallbladders of humans and mouse models of chronic carriage. individual roles of matrix components have not been completely elucidated in vitro or in vivo to examine individual functions, strains of salmonella enterica serovar typhimurium, the murine model of s typhi, in which various ecm gene ... | 2016 | 27600501 |
| higher storage temperature causes greater salmonella enterica serovar typhimurium internal penetration of artificially contaminated, commercially available, washed free range eggs. | foodborne salmonellosis is a major public health concern, with contaminated eggs identified as a significant source of infection. in australia, the most prevalent cause of salmonellosis from eggs is salmonella enterica subsp. enterica serovar typhimurium. this study explored the effect of temperature after 1, 7, 14, 21, and 28 days of storage on commercially available washed free range eggs, artificially contaminated with salmonella typhimurium on the external surface. at each time point, the ex ... | 2016 | 27357046 |
| antimicrobial resistance and class 1 integron-associated gene cassettes in salmonella enterica serovar typhimurium isolated from pigs at slaughter and abattoir environment. | forty-five multi-resistant salmonella enterica subsp. enterica serovar (s.) typhimurium isolates obtained at five pig abattoirs in southern brazil were characterized. their relatedness was determined by xbai-macrorestriction analysis. resistance genes, integrons and plasmid-mediated quinolone resistance genes (pmqr) were investigated by pcr. amplicons for the variable part of class 1 integrons and the quinolone resistance-determining regions (qrdr) were sequenced. plasmids were characterized by ... | 2016 | 27142182 |
| genome sequence of bacteriophage gg32, which can infect both salmonella enterica serovar typhimurium and escherichia coli o157:h7. | we report here a new virulent salmonella enterica serovar typhimurium (s typhimurium) bacteriophage, gg32, which was isolated from the guem river in the republic of korea. the strain can infect both s typhimurium and escherichia coli (e. coli) o157:h7 and may be a good candidate for a bio-control agent. | 2016 | 27932635 |
| molecular profiling: catecholamine modulation of gene expression in escherichia coli o157:h7 and salmonella enterica serovar typhimurium. | investigations of escherichia coli o157:h7 and salmonella enterica serovar typhimurium have demonstrated that these bacterial pathogens can respond to the presence of catecholamines including norepinephrine and/or epinephrine in their environment by modulating gene expression and exhibiting various phenotypes. for example, one of the most intensively investigated phenotypes following exposure of e. coli and s. typhimurium to norepinephrine is enhanced bacterial growth in a serum-based medium. ho ... | 2016 | 26589218 |
| intermediate filaments enable pathogen docking to trigger type 3 effector translocation. | type 3 secretion systems (t3sss) of bacterial pathogens translocate bacterial effector proteins that mediate disease into the eukaryotic cytosol. effectors traverse the plasma membrane through a translocon pore formed by t3ss proteins. in a genome-wide selection, we identified the intermediate filament vimentin as required for infection by the t3ss-dependent pathogen s. flexneri. we found that vimentin is required for efficient t3ss translocation of effectors by s. flexneri and other pathogens t ... | 2016 | 27572444 |
| complete genome sequence of a myoviridae bacteriophage infecting salmonella enterica serovar typhimurium. | the bacteriophage 118970_sal3 was isolated from water buffalo feces in southern italy, exhibiting lytic activity against salmonella enterica serovar typhimurium. this bacteriophage belongs to the myoviridae family and has a 39,464-bp double-stranded dna (ds-dna) genome containing 53 coding sequences (cdss). | 2016 | 27688333 |
| complete genome sequence of salmonella enterica serovar typhimurium strain so2 (sequence type 302) isolated from an asymptomatic child in mexico. | the complete genome sequence ofsalmonella entericaserovar typhimurium strain so2, isolated from an asymptomatic child in mexico, was determined using pacbio single-molecule real-time technology. strain so2 has six complete chromosomal prophages, namely, st104, gifsy-2, st64b, gifsy-1, elphis, and fsl sp-004, and carries asalmonellavirulence plasmid. | 2016 | 27081133 |
| curcumin reduces the motility of salmonella enterica serovar typhimurium by binding to the flagella, thereby leading to flagellar fragility and shedding. | one of the important virulence properties of the pathogen is its ability to travel to a favorable environment, cross the viscous mucus barrier (intestinal barrier for enteric pathogens), and reach the epithelia to initiate pathogenesis with the help of an appendage, like flagella. nonetheless, flagella can act as an "achilles heel," revealing the pathogen's presence to the host through the stimulation of innate and adaptive immune responses. we assessed whether curcumin, a dietary polyphenol, co ... | 2016 | 27091154 |
| cobalamin production by lactobacillus coryniformis: biochemical identification of the synthetized corrinoid and genomic analysis of the biosynthetic cluster. | despite the fact that most vitamins are present in a variety of foods, malnutrition, unbalanced diets or insufficient intake of foods are still the cause of vitamin deficiencies in humans in some countries. vitamin b12 (cobalamin) is a complex compound that is only naturally produced by bacteria and archea. it has been reported that certain strains belonging to lactic acid bacteria group are capable of synthesized water-soluble vitamins such as those included in the b-group, as vitamin b12. in t ... | 2016 | 27737643 |
| prevalence, seasonal occurrence, and antimicrobial resistance of salmonella spp. isolates recovered from chicken carcasses sampled at major poultry processing plants of south korea. | the current study was conducted to assess salmonella spp. contamination in chicken carcasses produced at major poultry processing plants in south korea. in total, 120 chicken carcasses were collected through 12 individual trials (10 chickens per trial) from six poultry processing plants in the summer of 2014 and the winter of 2015. eighteen chicken samples (15%) were contaminated with salmonella, with a higher rate of contamination observed during summer (14 isolates, 11.7%) than during winter ( ... | 2016 | 27442349 |
| application of antimicrobial agents via commercial spray cabinet to inactivate salmonella on skinless chicken meat. | salmonella enterica subsp. enterica serovar typhimurium is a food safety concern for raw poultry products. new and innovative application methods of antimicrobials for the reduction of salmonella in poultry and poultry products are essential. the aim of this study was to determine the efficacy of three antimicrobial compounds against salmonella on raw chicken meat when applied individually and in combination using a commercial spray cabinet. raw chicken thigh meat inoculated with 5 log cfu/g sal ... | 2016 | 27052860 |
| characterization of virulence-associated genes, antimicrobial resistance genes, and class 1 integrons in salmonella enterica serovar typhimurium isolates from chicken meat and humans in egypt. | foodborne pathogens are leading causes of illness especially in developing countries. the current study aimed to characterize virulence-associated genes and antimicrobial resistance in 30 salmonella typhimurium isolates of chicken and human origin at mansoura, egypt. the results showed that inva, avra, mgtc, stn, and bcfc genes were identified in all the examined isolates, while 96.7% and 6.7% were positive for sopb and pef genes, respectively. the highest resistance frequencies of the isolates ... | 2016 | 26977940 |
| novel two-step hierarchical screening of mutant pools reveals mutants under selection in chicks. | contaminated chicken/egg products are major sources of human salmonellosis, yet the strategies used by salmonella to colonize chickens are poorly understood. we applied a novel two-step hierarchical procedure to identify new genes important for colonization and persistence of salmonella enterica serotype typhimurium in chickens. a library of 182 s. typhimurium mutants each containing a targeted deletion of a group of contiguous genes (for a total of 2,069 genes deleted) was used to identify regi ... | 2016 | 26857572 |
| ex vivo perfusion of the isolated rat small intestine as a novel model of salmonella enteritis. | using an ex vivo perfused rat small intestinal model, we examined pathological changes to the tissue, inflammation induction, as well as dynamic changes to smooth muscle activity, metabolic competence, and luminal fluid accumulation during short-term infection with the enteropathogenic bacteria salmonella enterica serovar typhimurium and yersinia enterocolitica. although few effects were seen upon yersinia infection, this system accurately modeled key aspects associated with salmonella enteritis ... | 2016 | 26564721 |
| pleural empyema due to salmonella enterica serovar enteritidis in an immunocompetent elderly patient: a case report. | pleural empyema as a focal infection due to salmonella enterica serovar enteritidis is rare and most commonly described among immunosuppressed patients or patients who suffer from sickle cell anaemia and lung malignancies. | 2016 | 28348773 |
| complete, closed genome sequences of 10 salmonella enterica subsp. enterica serovar typhimurium strains isolated from human and bovine sources. | salmonella enterica is a leading cause of enterocolitis for humans and animals. s. enterica subsp. enterica serovar typhimurium infects a broad range of hosts. to facilitate genomic comparisons among isolates from different sources, we present the complete genome sequences of 10 s typhimurium strains, 5 each isolated from human and bovine sources. | 2016 | 27811097 |
| genomic analysis of salmonella enterica serovar typhimurium from wild passerines in england and wales. | passerine salmonellosis is a well-recognized disease of birds in the order passeriformes, which includes common songbirds such as finches and sparrows, caused by infection with salmonella enterica serovar typhimurium. previous research has suggested that some subtypes of s typhimurium-definitive phage types (dts) 40, 56 variant, and 160-are host adapted to passerines and that these birds may represent a reservoir of infection for humans and other animals. here, we have used the whole-genome sequ ... | 2016 | 27613688 |
| novel approach of a phage-based magnetoelastic biosensor for the detection of salmonella enterica serovar typhimurium in soil. | to date, there has been no employment of a magnetoelastic (me) biosensor method to detect salmonella enterica serovar typhimurium in soil. the me biosensor method needs to be investigated and modified for its successful performance. the filtration method, cation-exchange resin method, and combinations of both methods were employed for the extraction of s. typhimurium from soil. the number of s. typhimurium and the resonant frequency shift of the me sensor were then compared using a brilliant gre ... | 2016 | 27728960 |
| a case study on salmonella enterica serovar typhimurium at a dairy farm associated with massive sparrow death. | salmonella enterica typhimurium (s. typhimurium) is the most common cause of bovine salmonellosis in japan and where it is also cause of salmonellosis in wild birds. in 2008, a postpartum cow at a dairy farm developed diarrhea caused by s. typhimurium. the herd was extensively surveilled for salmonella sp. and we characterized bacterial isolates from this and other cows to determine the source of infection. | 2016 | 27112780 |
| wild-type and mutant avra- salmonella induce broadly similar immune pathways in the chicken ceca with key differences in signaling intermediates and inflammation. | salmonella enterica serovar typhimurium (st) is a serious infectious disease throughout the world, and a major reservoir for salmonella is chicken. chicken infected with salmonella do not develop clinical disease, this may be the result of important host interactions with key virulence proteins. to study this, we inoculated chicken with mutant salmonella typhimurium that lacked the virulence protein avra (avra(-)). avra is referred to as an avirulence factor, as it moderates the host immune resp ... | 2016 | 26574031 |
| the effect of recombinant human lactoferrin from the milk of transgenic cows on salmonella enterica serovar typhimurium infection in mice. | lactoferrin (lf) is a multifunctional protein with antibacterial and immunomodulatory activities. given this beneficial effect, transgenic approaches have been used to produce lactoferrin. the aim of the current study was to investigate the in vivo effect of recombinant human lactoferrin (rhlf) from the milk of transgenic cows on salmonella enterica serovar typhimurium (st) infection in mice. two hours before the infection with 0.3 ml at 2 × 10(5) cfu ml(-1) of st, each animal in the st + rhlf g ... | 2016 | 26469086 |
| breast abscess due to salmonella typhimurium in a patient with rheumatoid arthritis: a case report. | this is the first report of breast abscess due to salmonella enterica serotype typhimurium. staphylococcus aureus is known as the most common cause of breast abscess. salmonella spp. may occasionally form localized abscesses after dissemination to various organ systems following a bacteraemia. but breast abscess related to salmonella spp is a very rare complication. | 2016 | 27448659 |
| the architecture of the cytoplasmic region of type iii secretion systems. | type iii secretion systems (t3sss) are essential devices in the virulence of many gram-negative bacterial pathogens. they mediate injection of protein effectors of virulence from bacteria into eukaryotic host cells to manipulate them during infection. t3sss involved in virulence (vt3sss) are evolutionarily related to bacterial flagellar protein export apparatuses (ft3sss), which are essential for flagellar assembly and cell motility. the structure of the external and transmembrane parts of both ... | 2016 | 27686865 |
| the antibacterial activity of coriolus versicolor methanol extract and its effect on ultrastructural changes of staphylococcus aureus and salmonella enteritidis. | the antibacterial activity of methanol extract obtained from fruiting body of industrially grown basidiomycete coriolus versicolor was examined. the minimum inhibitory concentration (mic) values against various bacteria ranged from 0.625 to 20 mg ml(-1). c. versicolor expressed bactericidal activity against both gram-positive and gram-negative bacteria. the growth curves of staphylococcus aureus and salmonella enterica serovar enteritidis, measured at 630 nm, and confirmed with macrodilution met ... | 2016 | 27540376 |
| whole-genome sequencing analysis of salmonella enterica serovar enteritidis isolates in chile provides insights into possible transmission between gulls, poultry, and humans. | salmonella enterica subsp. enterica serotype enteritidis is a major cause of human salmonellosis worldwide; however, little is known about the genetic relationships between s enteritidis clinical strains and s enteritidis strains from other sources in chile. we compared the whole genomes of 30 s enteritidis strains isolated from gulls, domestic chicken eggs, and humans in chile, to investigate their phylogenetic relationships and to establish their relatedness to international strains. core geno ... | 2016 | 27520817 |
| effects of salmonella infection on hepatic damage following acute liver injury in rats. | acute liver injury is a common clinical disorder associated with intestinal barrier injury and disturbance of intestinal microbiota. probiotic supplementation has been reported to reduce liver injury; however, it is unclear whether enteropathogen infection exacerbates liver injury. the purpose of this study was to address this unanswered question using a rat model. | 2016 | 27498580 |
| chicken-specific kinome array reveals that salmonella enterica serovar enteritidis modulates host immune signaling pathways in the cecum to establish a persistence infection. | non-typhoidal salmonella enterica induces an early, short-lived pro-inflammatory response in chickens that is asymptomatic of clinical disease and results in a persistent colonization of the gastrointestinal (gi) tract that transmits infections to naïve hosts via fecal shedding of bacteria. the underlying mechanisms that control this persistent colonization of the ceca of chickens by salmonella are only beginning to be elucidated. we hypothesize that alteration of host signaling pathways mediate ... | 2016 | 27472318 |
| draft genome sequences of salmonella enterica subsp. enterica serovars typhimurium and nottingham isolated from food products. | a quantitative real-time pcr (qpcr) designed to detect salmonella enterica subsp. enterica serovar enteritidis, targeting the sdf gene, generated positive results for s. enterica subsp. enterica serovar typhimurium (cfsan033950) and s. enterica subsp. enterica serovar nottingham (cfsan006803) isolated from food samples. both strains show pulsed-field gel electrophoresis (pfge) patterns distinct from those of s enteritidis. here, we report the genome sequences of these two strains. | 2016 | 27445384 |
| salmonella enterica serovar enteritidis brain abscess mimicking meningitis after surgery for glioblastoma multiforme: a case report and review of the literature. | salmonella brain abscess associated with brain tumor is rare. only 11 cases have been reported to date. here we report a case of brain abscess caused by salmonella enterica serovar enteritidis mimicking post-surgical meningitis in a patient with glioblastoma multiforme. | 2016 | 27387824 |
| salmosan, a β-galactomannan-rich product, protects epithelial barrier function in caco-2 cells infected by salmonella enterica serovar enteritidis. | one promising strategy for reducing human salmonellosis induced by salmonella enteritidis is to supplement animal diets with natural feed additives such as mannan oligosaccharides (moss). | 2016 | 27358412 |
| o-antigen chain-length distribution in salmonella enterica serovar enteritidis is regulated by oxygen availability. | lipopolysaccharide (lps) consists of three covalently linked domains: the lipid a, the core region and the o antigen (oag), consisting of repeats of an oligosaccharide. salmonella enterica serovar enteritidis (s. enteritidis) produces a lps with two oag preferred chain lengths: a long (l)-oag controlled by wzzse and a very long (vl)-oag controlled by wzzfepe. in this work, we show that oag produced by s. enteritidis grown in e minimal medium also presented two preferred chain-lengths. however, a ... | 2016 | 27343553 |
| spi-9 of salmonella enterica serovar typhi is constituted by an operon positively regulated by rpos and contributes to adherence to epithelial cells in culture. | the genomic island 9 (spi-9) from salmonella enterica serovar typhi (s. typhi) carries three orfs (sty2876, sty2877, sty2878) presenting 98 % identity with a type 1 secretory apparatus (t1ss), and a single orf (sty2875) similar to a large rtx-like protein exhibiting repeated ig domains. bapa, the salmonella enterica serovar enteritidis orthologous to s. typhi sty2875, has been associated with biofilm formation, and is described as a virulence factor in mice. preliminary in silico analyses reveal ... | 2016 | 27260307 |
| whole-genome sequence of salmonella enterica serovar enteritidis phage type 4, isolated from a brazilian poultry farm. | the draft genome of salmonella enterica serovar enteritidis phage type 4 (pt4) strain ioc4647/2004, isolated from a poultry farm in são paulo state, was obtained with high-throughput illumina sequencing platform, generating 4,173,826 paired-end reads with 251 bp. the assembly of 4,804,382 bp in 27 scaffolds shows strong similarity to other s enteritidis strains. | 2016 | 27174265 |
| the salmonella pathogenicity island 13 contributes to pathogenesis in streptomycin pre-treated mice but not in day-old chickens. | salmonella enterica serovar enteritidis (s. enteritidis) is a human and animal pathogen that causes gastroenteritis characterized by inflammatory diarrhea and occasionally an invasive systemic infection. salmonella pathogenicity islands (spis) are horizontally acquired genomic segments known to contribute to salmonella pathogenesis. the objective of the current study was to determine the contribution of spi-13 to s. enteritidis pathogenesis. | 2016 | 27141235 |
| salmonella typhimurium and outbreaks of egg-associated disease in australia, 2001 to 2011. | salmonellosis is a significant public health problem, with eggs frequently identified as a food vehicle during outbreak investigations. salmonella enterica serovar typhimurium and salmonella enterica serovar enteritidis are the two most frequently identified causes of egg-associated disease in industrialized countries. in australia, a comprehensive review of egg-associated outbreaks has not been previously undertaken. | 2016 | 27028267 |
| functional characterization of glucosamine-6-phosphate synthase (glms) in salmonella enterica serovar enteritidis. | salmonella is a threat to public health due to consumption of contaminated food. screening of a transposon library identified a unique mutant that was growth and host cell binding deficient. the objective of this study was to determine the functional role of glucosamine-6-phosphate synthase (glms) in the biology and pathogenesis of salmonella. to examine this, we created a glms mutant (δglms) of salmonella and examined the effect on cell envelope integrity, growth, metabolism, and pathogenesis. ... | 2016 | 27017337 |
| the impact of salmonella enteritidis on lipid accumulation in chicken hepatocytes. | salmonella enterica serovar enteritidis (se) is a public health concern and infected chickens serve as a reservoir that potentially transmits to humans through food. although se seldom causes systemic disease in chickens, virulent se strains can colonize in intestines and lead a persistent infection of the liver. the liver is the primary organ for lipid metabolism in chickens and the site for production and assembly of main components in yolk. we performed a time-course experiment using lmh-2a c ... | 2016 | 26957042 |
| highly expressed amino acid biosynthesis genes revealed by global gene expression analysis of salmonella enterica serovar enteritidis during growth in whole egg are not essential for this growth. | salmonella enterica serovar enteritidis (s. enteritidis) is the most common cause of egg borne salmonellosis in many parts of the world. this study analyzed gene expression of this bacterium during growth in whole egg, and whether highly expressed genes were essential for the growth. high quality rna was extracted from s. enteritidis using a modified rna-extraction protocol. global gene expression during growth in whole egg was compared to growth in lb-medium using dna array method. twenty-six g ... | 2016 | 26945769 |
| validation and identification of invasive salmonella serotypes in sub-saharan africa by multiplex polymerase chain reaction. | salmonella enterica serovar typhi and nontyphoidal salmonella (nts) cause the majority of bloodstream infections in sub-saharan africa; however, serotyping is rarely performed. we validated a multiplex polymerase chain reaction (pcr) assay with the white-kauffmann-le minor (wklm) scheme of serotyping using 110 salmonella isolates from blood cultures of febrile children in ghana and applied the method in other typhoid fever surveillance in africa program study sites. in ghana, 47 (43%) s. typhi, ... | 2016 | 26933026 |
| the emergence of reduced ciprofloxacin susceptibility in salmonella enterica causing bloodstream infections in rural ghana. | salmonella ranks among the leading causes of bloodstream infections in sub-saharan africa. multidrug resistant typhoidal and nontyphoidal salmonella (nts) isolates have been previously identified in this region. however, resistance to ciprofloxacin has rarely been reported in west africa. this study aims to assess susceptibility against ciprofloxacin in salmonella causing invasive bloodstream infections among children in rural ghana. | 2016 | 26933017 |
| comparative study of all salmonella enterica serovar enteritidis strains isolated from food and food animals in greece from 2008 to 2010 with clinical isolates. | the aim of the present work was to study the epidemiology of salmonella enterica serovar enteritidis (s. enteritidis) in greece, comparing all the food and food animal isolates during a 3-year period with clinical isolates. submission of the generated data to the pulsenet europe database was carried out in order to study the population structure of this particular serovar and indicate possible connections with european strains. one hundred and sixty-eight (168) s. enteritidis strains of human, a ... | 2016 | 26864044 |
| genetic diversity and antimicrobial resistance pattern of salmonella enterica serovar enteritidis clinical isolates in thailand. | to trace the history of antimicrobial resistance in salmonella enterica serovar enteritidis (s. enteritidis, se) circulating in thailand, we characterised clinical isolates obtained during 2004-2007. | 2016 | 26860961 |
| loop-mediated isothermal amplification of the sefa gene for rapid detection of salmonella enteritidis and salmonella gallinarum in chickens. | salmonella spp. pose a threat to both human and animal health, with more than 2600 serovars having been reported to date. salmonella serovars are usually identified by slide agglutination tests, which are labor intensive and time consuming. in an attempt to develop a more rapid screening method for the major poultry salmonella serovars, we developed a loop-mediated isothermal amplification (lamp) assay, which directly detected the sefa gene, a fimbrial operon gene existing in several specific se ... | 2016 | 26840841 |
| the evaluation and application of multilocus variable number tandem repeat analysis (mlva) for the molecular epidemiological study of salmonella enterica subsp. enterica serovar enteritidis infection. | salmonella enterica subsp. enterica serovar enteritidis (s. enteritidis) is one of the most prevalent salmonella serotypes that cause gastroenteritis worldwide and the most prevalent serotype causing salmonella infections in china. a rapid molecular typing method with high throughput and good epidemiological discrimination is urgently needed for detecting the outbreaks and finding the source for effective control of s. enteritidis infections. | 2016 | 26823185 |
| ethanol adaptation induces direct protection and cross-protection against freezing stress in salmonella enterica serovar enteritidis. | salmonella enterica serovar enteritidis (salm. enteritidis) encounters mild ethanol stress during its life cycle. however, adaptation to a stressful condition may affect bacterial resistance to subsequent stresses. hence, this work was undertaken to investigate the influences of ethanol adaptation on stress tolerance of salm. enteritidis. | 2016 | 26743544 |
| an unusual case of salmonella enteritidis causing pneumonia, septic shock and multiple organ failure in an immunocompetent patient. | salmonella species are facultative intracellular pathogens that most frequently cause self-limiting gastrointestinal disease, often acquired through the ingestion of contaminated food. we report the case of a 33-year-old otherwise healthy, not overtly immunosuppressed, man who was transferred to our facility with the chief complaint of respiratory failure and septic shock. computed tomography of the chest revealed multifocal pneumonia in both lungs. a bronchial alveolar lavage was performed in t ... | 2016 | 27818944 |
| longitudinal monitoring of successive commercial layer flocks for salmonella enterica serovar enteritidis. | the pennsylvania egg quality assurance program (eqap) provided the framework for salmonella enteritidis (se) control programs, including the food and drug administration (fda) mandated final egg rule, for commercial layer facilities throughout the united states. although flocks with ≥3000 birds must comply with the fda final egg rule, smaller flocks are exempted from the rule. as a result, eggs produced by small layer flocks may pose a greater public health risk than those from larger flocks. it ... | 2016 | 27792449 |