Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| [analysis on vp1 gene variation of coxsackie virus b5 for aseptic meningitis isolated from zhejiang province in 2008]. | study meningoencephalitis virus isolated from the coxsackie b5 virus(cvb5 virus) vp1 gene characteristics of zhejiang province in 2008 and compare with other countries cvb5 prototype isolates, and to explore the relationship of variation of the virus vp1 areas and epidemic viral meningoencephalitis | 2010 | 20450071 |
| the 3c protein of enterovirus 71 inhibits retinoid acid-inducible gene i-mediated interferon regulatory factor 3 activation and type i interferon responses. | enterovirus 71 (ev71) is a human pathogen that induces hand, foot, and mouth disease and fatal neurological diseases. immature or impaired immunity is thought to associate with increased morbidity and mortality. in a murine model, ev71 does not facilitate the production of type i interferon (ifn) that plays a critical role in the first-line defense against viral infection. administration of a neutralizing antibody to ifn-alpha/beta exacerbates the virus-induced disease. however, the molecular ev ... | 2010 | 20519382 |
| producing infectious enterovirus type 71 in a rapid strategy. | enterovirus 71 (ev71) is an etiologic agent of hand-foot-and-mouth disease (hfmd), and recent hfmd epidemics worldwide have been associated with a severe form of brainstem encephalitis associated with pulmonary edema and high case-fatality rates. ev71 contains a positive-sense single-stranded genome rna of approximately 7400 bp in length which encodes a polyprotein with a single open reading frame (orf), which is flanked by untranslated regions at both the 5' and 3' ends. | 2010 | 20525351 |
| regulating viral myocarditis: allografted regulatory t cells decrease immune infiltration and viral load. | 2010 | 20529996 | |
| regulatory t cells protect mice against coxsackievirus-induced myocarditis through the transforming growth factor beta-coxsackie-adenovirus receptor pathway. | coxsackievirus b3 infection is an excellent model of human myocarditis and dilated cardiomyopathy. cardiac injury is caused either by a direct cytopathic effect of the virus or through immune-mediated mechanisms. regulatory t cells (tregs) play an important role in the negative modulation of host immune responses and set the threshold of autoimmune activation. this study was designed to test the protective effects of tregs and to determine the underlying mechanisms. | 2010 | 20530002 |
| genomic characterization of an enterovirus 97 strain isolated in shandong, china. | the genomic characterization of human enterovirus 97 (ev97) strain isolated from an acute flaccid paralysis case in shandong province, china in 1999, is described. the strain, designated as 99188/sd/chn/1999/ev97 (abbreviated as 99188), had a genome of 7394 nucleotides. compared with other ev97 strains, it had 81.3-83.3% nucleotide similarity and 94.0-95.4% amino acid similarity in vp1 coding region, and it had 81.4% complete genomic similarity with prototype strain ban99-10355. the most strikin ... | 2010 | 20532813 |
| [the heart in viral infections]. | between 10 and 20% of patients with histologically proven inflammatory disease of the heart muscle develop a chronic disorder after acute myocarditis which results in dilated cardiomyopathy with increasing cardiac insufficiency. viral infections are a frequent cause of inflammatory heart muscle diseases and thus also responsible for myocardial damage in the initial phase. in the past, evidence for enterovirus, adenovirus, and cytomegalovirus was in the focus of attention. in the meantime, "new" ... | 2010 | 20532860 |
| [epidemiological and clinical features of enteroviral meningitis: a case series of 59 adult patients]. | to describe the epidemiological and clinical features of enteroviral meningitis as well as the biological profile of the cerebrospinal fluid (csf). | 2010 | 20537440 |
| type i interferon signals control theiler's virus infection site, cellular infiltration and t cell stimulation in the cns. | theiler's murine encephalomyelitis virus (tmev) establishes a persistent infection in the central nervous system (cns). to examine the role of type i interferon (ifn-i)-mediated signals in tmev infection, mice lacking a subunit of the type i ifn receptor (ifn-ir ko mice) were utilized. in contrast to wild type mice, ifn-ir ko mice developed rapid fatal encephalitis accompanied with greater viral load and infiltration of immune cells to the cns. the proportion of virus-specific cd4(+) and cd8(+) ... | 2010 | 20538350 |
| respiratory echovirus 30 and coxsackievirus b5 can induce production of rantes, mcp-1 and il-8 by human bronchial epithelial cells. | human enteroviruses (hev) (picornaviridae) are considered as one the major viral causes of childhood acute respiratory wheezing illnesses including bronchiolitis and asthma exacerbation. to identify the mechanisms that can regulate the development of airway mucosa inflammation during hev respiratory lower tract infection, we investigated the profile and the levels of mrna and protein secretion for cc and cxc human chemokines by hev-infected primary human bronchial epithelial cells (sae cells) us ... | 2010 | 20540976 |
| an investigation of epidemic enterovirus 71 infection in taiwan, 2008: clinical, virologic, and serologic features. | enterovirus 71 (ev71) is causing life-threatening hand-foot-mouth disease in asia. in taiwan, ev71 epidemics with different predominant genotypes occurred in 1998 (c2), 2000-2001 (b4), and 2004-2005 (c4). this genotype replacement may have important implications for vaccine development and prediction of epidemics. a nationwide ev71 outbreak occurred again in 2008, which provided a unique opportunity to characterize clinical, virologic, and serologic features of this epidemic. | 2010 | 20543760 |
| transient supranuclear paresis of the abduction in viral encephalitis of the brainstem. | the supranuclear paresis of the abducens system, also known as posterior internuclear ophthalmoplegia of abduction, is a very rare disorder clinically characterized by unilateral or bilateral abduction paresis sometimes associated with nystagmus of the contralateral adducting eye, slowing of abduction saccades, and intact horizontal vestibulo-ocular reflex. here, we report a 35-year-old woman who presented transient left side abduction deficit in conjunction, as the only symptom of self-limited ... | 2010 | 20544248 |
| use of preconditioned human phagocytes for extracorporeal adsorption of viruses. | conventional treatment of severe viral disease is limited by the narrow choice as well as the often-significant side effects or lack of clear efficacy of antiviral chemotherapy. at the same time, however, it is known that a reduction in viral load leads to significant clinical improvement in a number of important viral diseases. in this paper we discuss the possibility of using preconditioned human phagocytes in an extracorporeal biohybrid system for adsorption of viral pathogens. we present dat ... | 2010 | 20546790 |
| emergence of enterovirus 71 "double-recombinant" strains belonging to a novel genotype d originating from southern china: first evidence for combination of intratypic and intertypic recombination events in ev71. | hand-foot-mouth disease due to enterovirus 71 (ev71) and coxsackievirus a16 (ca16) has recently caused large outbreaks in mainland china in 2008. we performed complete genome sequencing on two ev71 (sz/hk08-5 and sz/hk08-6) and two ca16 (sz/hk08-3 and sz/hk08-7) strains from patients in shenzhen, china. phylogenetic, similarity plot and bootscan analyses revealed recombination between ev71 genotypes b and c at the 2a-2b junction, and between ev71 genotype b and ca16 strain g-10 in the 3c region ... | 2010 | 20549263 |
| [etiology of hand, foot and mouth disease in guangzhou in 2008]. | to understand the etiology of hand, foot and mouth disease (hfmd) in guangzhou area in 2008. | 2010 | 20426938 |
| [autopsy report of two cases with enterovirus type 71 infection brainstem encephalitis and literature review]. | to understand clinicopathologic characteristics of hand-foot-and-mouth disease. | 2010 | 20426961 |
| hiv-related opsoclonus-myoclonus-ataxia syndrome: report on two cases. | opsoclonus-myoclonus-ataxia (oma) syndrome is a rare neurological disorder, characterized by a rapid onset of generalized myoclonus in association with chaotic multi-directional eye movements and, less frequently, cerebellar ataxia. oma is commonly related to a paraneoplastic process, specifically neuroblastoma in children and lung or breast cancer in adults. nevertheless, oma may occur in association with various infectious agents, such as coxsackie virus b3, epstein-barr virus, mumps, enterovi ... | 2010 | 20427123 |
| enterovirus infection of human beta-cells activates dendritic cells and triggers innate antiviral responses: are enteroviruses convicted now? | 2010 | 20427698 | |
| [detection of enteric viruses in wastewater of monastir region by rt-pcr method]. | wastewater frequently has been contaminated by enteric viruses, which can cause gastroenteritis, hepatitis, and other viral diseases. since current water treatments do not ensure their complete removal and they become contaminants of the water environment. | 2010 | 20415161 |
| 99th dahlem conference on infection, inflammation and chronic inflammatory disorders: viruses, autoimmunity and immunoregulation. | based on studies in animal models, viral infections, in particular by enteroviruses, can accelerate or halt type 1 diabetes (t1d) development. among factors that determine the outcome are the degree of viral replication in the target organ (viral titres), the tropism of the virus for beta cells, and the precise time-point of infection in relation to the diabetogenic process. mechanisms underlying these phenomena have been assessed in mouse studies and should now be verified for human t1d. for en ... | 2010 | 20415860 |
| increased risk of adverse pregnancy outcomes among women affected by herpangina. | this population-based study aimed to assess the relation between herpangina and adverse pregnancy outcomes: low birthweight (lbw), small for gestational age (sga), and preterm delivery. | 2010 | 20417474 |
| molecular beacon-quantum dot-au nanoparticle hybrid nanoprobes for visualizing virus replication in living cells. | here we describe a new hybrid fluorescent nanoprobe composed of a nuclease-resistant molecular beacon (mb) backbone, cdse-zns core-shell quantum dots (qds) as donors, and gold nanoparticles (au nps) as quenchers, for the real-time visualization of virus replication in living cells. by using a au np-mb to qd ratio of 6 : 1, a 7.3-fold increase in fluorescent signal was achieved upon target binding. for living cell experiments, a hexahistidine-appended tat peptide was self-assembled onto the qd su ... | 2010 | 20419176 |
| genetic characterization of norovirus gii.4 2006b variants from jeju island, south korea. | ninety-seven fecal specimens collected from children with acute gastroenteritis between 2007 and 2008 that were found to be negative for group a rotavirus in prescreening by elisa with vp6-specific antibody were re-screened for viruses by reverse transcription (rt)-pcr. forty (41.2%) samples were found to be positive for virus by rt-pcr; of these, norovirus (32.5%, n = 13) and rotavirus (32.5%, n = 13) were the most common, followed by astrovirus (5.0%, n = 2) and enterovirus (2.5%, n = 1). co-i ... | 2010 | 20419823 |
| antiviral effect of bosentan and valsartan during coxsackievirus b3 infection of human endothelial cells. | in viral myocarditis, adeno- and enteroviruses have most commonly been implicated as causes of infection. both viruses require the human coxsackie-adenovirus receptor (car) to infect the myocardium. due to its crucial role for viral entry, car-downregulation may lead to novel approaches for treatment for viral myocarditis. in this study, we report on pharmaceutical drug influences on car levels in human umbilical vein endothelial cells (huvec) and cervical carcinoma cells (hela) detected by immu ... | 2010 | 20392896 |
| [detection of coxsackie b3 virus infection in newborn care unit using reverse transcriptase polymerase chain reaction method]. | to explore the sensitivity of reverse transcriptase polymerase chain reaction (rt-pcr) method for detection of coxsackie virus b3 (coxb3) infection. | 2010 | 20396370 |
| picornaviruses. | the picornavirus family consists of a large number of small rna viruses, many of which are significant pathogens of humans and livestock. they are amongst the simplest of vertebrate viruses comprising a single stranded positive sense rna genome within a t = 1 (quasi t = 3) icosahedral protein capsid of approximately 30 nm diameter. the structures of a number of picornaviruses have been determined at close to atomic resolution by x-ray crystallography. the structures of cell entry intermediate pa ... | 2010 | 20397067 |
| detection and quantification of enteroviruses in coastal seawaters from bohai bay, tianjin, china. | an 8-month survey was conducted to detect and quantify enteroviruses in tianjin coastal seawaters of bohai bay to assess coastal water quality. ten water samples were collected from bohai bay for the detection and quantification of enteroviruses by conventional reverse transcription polymerase chain reaction (rt-pcr) and sybr green real-time quantitative rt-pcr (qrt-pcr). total viral nucleic acid was extracted from 500 ml of seawater samples concentrated by centricon plus-70 centrifugal filter d ... | 2010 | 20397399 |
| sequence analysis of six enterovirus 71 strains with different virulences in humans. | enterovirus 71 (ev71) infection is the main cause of hand, foot and mouth disease (hfmd) and has been associated with severe neurological diseases resulting in high mortalities. in this study, six ev71 strains isolated from patients with different clinical symptoms were sequenced and analyzed in a mouse model of ev71 infection. in a phylogenetic tree, based on the complete vp1 gene sequence, all six strains grouped into the c4 genotype. the sequence analysis revealed that there are nucleotide ch ... | 2010 | 20398708 |
| molecular epidemiology of coxsackievirus b3. | molecular epidemiological characteristics are needed to understand the impact of coxsackievirus b3 (cv-b3) infection, since no cv-b3 genotyping literature is available. twenty-nine cv-b3 taiwan strains obtained from 1992 to 2005 were analyzed. a phylogenetic tree was constructed based on the 290 nucleotide sequence of the vp1 gene of taiwan isolates and in 91 other cv-b3 genbank sequences. five genotypes (gi-gv) were depicted. the gi, gii, and giii were dominant in america and europe, whereas gi ... | 2010 | 20398802 |
| enterovirus 71 vaccine: close but still far. | enterovirus 71 (ev71), a member of the enterovirus genus of the picornaviridae family, is one of the causative pathogens of hand-foot-and-mouth disease (hfmd) and the most common etiological agent isolated from hfmd patients complicated with neurological disorders. ev71 has become an increasingly important neurotropic enterovirus in the post-poliomyelitis eradication era. effective antiviral agents and vaccines against this virus are currently still under development. we reviewed publications on ... | 2010 | 20400350 |
| potential chemical and microbiological risks on human health from urban wastewater reuse in agriculture. case study of wastewater effluents in spain. | potential health risks derived from wastewater reuse in agriculture have been evaluated with risk assessment modelling techniques, in a case study involving the effluents of two spanish wastewater treatment plants. one of the plants applies primary and secondary treatment, and the other one applies an additional tertiary treatment. health risks were assessed on the basis of ingesting contaminated food, due to exposure to: (i) 22 chemical pollutants, namely pharmaceuticals and personal care produ ... | 2010 | 20401781 |
| enteroviral meningoencephalitis as complication of rituximab therapy in a patient treated for diffuse large b-cell lymphoma. | 2010 | 20408837 | |
| viral epidemiologic shift in inflammatory heart disease: the increasing involvement of parvovirus b19 in the myocardium of pediatric cardiac transplant patients. | detection of viral genome in rejecting cardiac transplant patients has been reported, with coxsackievirus and adenovirus causing premature graft failure. recently, parvovirus b19 (pvb19) genome in myocardial samples has been increasingly reported, but its role in cardiac pathology and effect on transplant graft survival are unknown. the objectives of this study were to determine if changes in the viruses identified in the myocardium represent an epidemiologic shift in viral myocardial disease an ... | 2010 | 20456978 |
| carvedilol treatment ameliorates acute coxsackievirus b3-induced myocarditis associated with oxidative stress reduction. | oxidative stress has been implicated in the pathogenesis of acute myocarditis. the imbalance between the occurrence of reactive oxygen species and the cellular antioxidant defense mechanism plays a key role in myocardial injury of viral myocarditis. carvedilol, a nonselective beta-adrenoceptor antagonist with additional alpha1-adrenergic blocking and antioxidant properties, has been shown to be cardioprotective in experimental myocarditis. however, the expression of 4-hydroxy-2-nonenal (4-hne), ... | 2010 | 20457150 |
| comparison of clinical features between coxsackievirus a2 and enterovirus 71 during the enterovirus outbreak in taiwan, 2008: a children's hospital experience. | coxsackievirus a2 (cox a2) was the predominant serotype in the enterovirus outbreak in taiwan, 2008. however, detailed clinical features of cox a2 infection have not been reported. in this study, we compared cox a2 with enterovirus 71 (ev71) in terms of clinical manifestation and epidemiology during the 2008 enterovirus outbreak in taiwan. | 2010 | 20457425 |
| an emerging recombinant human enterovirus 71 responsible for the 2008 outbreak of hand foot and mouth disease in fuyang city of china. | hand, foot and mouth disease (hfmd), a common contagious disease that usually affects children, is normally mild but can have life-threatening manifestations. it can be caused by enteroviruses, particularly coxsackieviruses and human enterovirus 71 (hev71) with highly variable clinical manifestations. in the spring of 2008, a large, unprecedented hfmd outbreak in fuyang city of anhui province in the central part of southeastern china resulted in a high aggregation of fatal cases. in this study, ... | 2010 | 20459851 |
| influence of cinnamaldehyde on viral myocarditis in mice. | this study was designed to examine the effects of cinnamaldehyde on coxsackievirus b3 (cvb3)-induced viral myocarditis (vmc) and underlying signaling, with a focus on the toll-like receptor (tlr)4-nuclear factor (nf)-kappab pathway. | 2010 | 20460981 |
| clinical and etiological characteristics of enterovirus 71-related diseases during a recent 2-year period in korea. | human enterovirus 71 (ev 71) has caused large-scale outbreaks of hand-foot-and-mouth disease (hfmd), particularly in the asian-pacific region. in this study, we report a major outbreak of ev 71 infection in korea and describe the clinical differences between ev 71 and non-ev 71 enterovirus infections. we prospectively enrolled patients with suspected viral infections during a recent 2-year period through a nationwide surveillance system. we identified 719 patients with suspected hfmd or herpangi ... | 2010 | 20463159 |
| [experimental study of antiviral activity of spore-forming bacterium bacillus pumilus "pashkov"]. | to study antiviral activity of metabolites of spore-forming strain "pashkov" of b. pumilus on the model of enterovirus infection in vitro. | 2010 | 20465005 |
| studies of echovirus 5 interactions with the cell surface: heparan sulfate mediates attachment to the host cell. | infections caused by echovirus 5 (e5), an enterovirus of the picornaviridae family, have been associated with fever, rashes and sporadic cases of aseptic meningitis. to elucidate the receptor usage of this virus, the significance of a previously proposed integrin binding arginine-glycine-aspartic acid (rgd) motif found in the vp3 capsid protein was investigated, as well as the capacity of e5 to interact with heparan sulfate on the cell surface. using the prototype strain e5 noyce (e5n), an e5n m ... | 2010 | 20466025 |
| a role for toll-like receptor 3 variants in host susceptibility to enteroviral myocarditis and dilated cardiomyopathy. | the innate antiviral response is mediated, at least in part, by toll-like receptors (tlrs). tlr3 signaling is activated in response to viral infection, and the absence of tlr3 in mice significantly increases mortality after infection with enteroviruses that cause myocarditis and/or dilated cardiomyopathy. we screened tlr3 in patients diagnosed with enteroviral myocarditis/cardiomyopathy and identified a rare variant in one patient as well as a significantly increased occurrence of a common polym ... | 2010 | 20472559 |
| use of propidium monoazide in reverse transcriptase pcr to distinguish between infectious and noninfectious enteric viruses in water samples. | human enteric viruses can be present in untreated and inadequately treated drinking water. molecular methods, such as the reverse transcriptase pcr (rt-pcr), can detect viral genomes in a few hours, but they cannot distinguish between infectious and noninfectious viruses. since only infectious viruses are a public health concern, methods that not only are rapid but also provide information on the infectivity of viruses are of interest. the intercalating dye propidium monoazide (pma) has been use ... | 2010 | 20472736 |
| florid facial rash due to insect bite in thailand or enterovirus in hong kong. or is it? | 2010 | 20500165 | |
| monoclonal antibody induced with inactived ev71-hn2 virus protects mice against lethal ev71-hn2 virus infection. | enterovirus 71 (ev71) is a viral pathogen that belongs to the picornaviridae family, ev71-infected children can develop severe neurological complications leading to rapid clinical deterioration and death. | 2010 | 20500892 |
| differential effects of the putative gbf1 inhibitors golgicide a and ag1478 on enterovirus replication. | the genus enterovirus, belonging to the family picornaviridae, includes well-known pathogens, such as poliovirus, coxsackievirus, and rhinovirus. brefeldin a (bfa) impedes replication of several enteroviruses through inhibition of golgi-specific bfa resistance factor 1 (gbf1), a regulator of secretory pathway integrity and transport. gbf1 mediates the gtp exchange of arf1, which in activated form recruits coatomer protein complex i (cop-i) to golgi vesicles, a process important in transport betw ... | 2010 | 20504936 |
| phylogenetic evidence for a recent spread of two populations of human enterovirus 71 in european countries. | human enterovirus 71 (ev-71) is a cause of seasonal epidemics of hand, foot and mouth disease, and of less common but severe neurological manifestations. uncertainty persists regarding the circulation of virus populations in several geographical areas and the timescale of their dissemination. we determined ev-71 sequences at loci 1d (vp1 capsid protein) and 3cd (non-structural proteins) in 86 strains recovered in austria, france and germany and performed an evolutionary genetic study of extant v ... | 2010 | 20505012 |
| enterovirus genotype ev-104 in humans, italy, 2008-2009. | in an epidemiologic investigation of respiratory infections in italy, october 2008-september 2009, we tested samples from patients for respiratory viruses. human enterovirus genotype ev-104 (identified in switzerland) was found in 3 immunocompromised and 2 immunocompetent patients. ev-104 is closely related to human rhinoviruses; thus, both types of viruses should be sought in respiratory syndromes. | 2010 | 20507762 |
| enteroviral encephalitis in a patient with a marginal zone lymphomatreated with rituximab. | a 64-year-old woman with a progressive marginal zone lymphoma for which she had received induction therapy with six courses of rituximab and fludarabine presented with fever while receiving maintenance therapy with rituximab. in addition to the fever she complained of nausea, vomiting, weight loss and fatigue. after an extensive diagnostic procedure no cause was found for the fever. finally, additional testing showed a positive polymerase chain reaction (pcr) for enterovirus in the cerebrospinal ... | 2010 | 20508271 |
| viral reorganization of the secretory pathway generates distinct organelles for rna replication. | many rna viruses remodel intracellular membranes to generate specialized sites for rna replication. how membranes are remodeled and what properties make them conducive for replication are unknown. here we show how rna viruses can manipulate multiple components of the cellular secretory pathway to generate organelles specialized for replication that are distinct in protein and lipid composition from the host cell. specific viral proteins modulate effector recruitment by arf1 gtpase and its guanin ... | 2010 | 20510927 |
| microrna antagonism of the picornaviral life cycle: alternative mechanisms of interference. | in addition to modulating the function and stability of cellular mrnas, micrornas can profoundly affect the life cycles of viruses bearing sequence complementary targets, a finding recently exploited to ameliorate toxicities of vaccines and oncolytic viruses. to elucidate the mechanisms underlying microrna-mediated antiviral activity, we modified the 3' untranslated region (3'utr) of coxsackievirus a21 to incorporate targets with varying degrees of homology to endogenous micrornas. we show that ... | 2010 | 20333250 |
| ev71 induces cox-2 expression via c-src/pdgfr/pi3k/akt/p42/p44 mapk/ap-1 and nf-kappab in rat brain astrocytes. | enterovirus 71 (ev71) induces the expression of cyclooxgenase (cox)-2 served as a major neurotoxic factor in cns injury. however, the mechanisms underlying ev71-initiated intracellular signaling pathways leading to cox-2 expression remain unknown. therefore, we investigated the mechanisms underlying ev71-induced cox-2 expression and prostaglandin e(2) (pge(2)) production in rat brain astrocytes (rba)-1, determined by western blotting, rt-pcr, and promoter assay. here, we reported that ev71-induc ... | 2010 | 20333648 |
| [role of toscana virus in meningo-encephalitis in tunisia.] | objectives: to detect the presence of toscana virus (tosv) circulation in tunisia and to study its role in viral meningo-encephalitis. patients and methods: a total of 315 (167 sera and 178 cerobrospinal fluid [csf]) samples was investigated. these samples are colleted from tunisian patients with neurological diseases during the period between january 2003 and december 2009. all samples were tested negative for enterovirus, herpes simplex virus and west nile virus. detection for igm and igg spec ... | 2010 | 20378279 |
| phylogenetic analysis of echovirus 30 isolated during the 2005 outbreak in france reveals existence of multiple lineages and suggests frequent recombination events. | echovirus 30 (e-30) was responsible in france for a major aseptic meningitis outbreak during 2005 summer season. however, the virological mechanisms responsible for the periodic emergence of the epidemic strains remain to be investigated. | 2010 | 20381415 |
| widespread circulation of echovirus type 13 demonstrated by increased seroprevalence in toyama, japan, between 2000 and 2003. | to confirm the magnitude of an echovirus type 13 (e13) outbreak in 2002 and to evaluate whether genetic and antigenic changes in e13 influenced the occurrence of the outbreak, we measured titers of neutralizing (nt) antibody against the toyama, 2002-240-sf, and prototype del carmen e13 strains among inhabitants of toyama before and after 2002. the rate of positivity for nt antibodies against both 2002-240-sf and del carmen in 2003 made a remarkable upturn in children 0 to 14 years old, compared ... | 2010 | 20335430 |
| the detection of enteric viruses in selected urban and rural river water and sewage in kenya, with special reference to rotaviruses. | to determine the occurrence of eight human enteric viruses in surface water and sewage samples from different geographical areas in kenya. | 2010 | 20337763 |
| neural pathogenesis of enterovirus 71 infection. | enterovirus 71 (ev71) is a neurotropic pathogen that can cause severe neural diseases and complications on infected patients. clinical observations showed that ev71-induced immune responses may be associated with virus induced neurogenic pulmonary edema. here reviewed studies that discovered several host molecules as potential factors for ev71 virulence. | 2010 | 20348010 |
| rituximab-associated infections. | after more than 10 years of use, rituximab has proven to be remarkably safe. however, accumulated evidence now suggests that under some circumstances it may significantly increase the risk of infections. this risk is difficult to quantify because of confounding factors (namely, concomitant use of immunosuppressive or chemotherapeutic agents and underlying conditions), as well as under-reporting. increased number of infections has been documented in patients treated with maintenance rituximab for ... | 2010 | 20350666 |
| circulating viruses associated with severe complicated enterovirus infection in taiwan: a multi-year analysis. | few studies have investigated the viral isolation characteristics for severe complicated enterovirus infection (scei). this study evaluated the seasonality and contribution of circulated viruses to the chronologic trend and weekly reported scei epidemic. | 2010 | 20351529 |
| pathogenesis of type 1 diabetes mellitus: interplay between enterovirus and host. | enteroviruses are believed to contribute to the pathogenesis of type 1 diabetes mellitus (t1dm). in this review, the interplay between infection with enteroviruses, the immune system and host genes is discussed. data from retrospective and prospective epidemiological studies strongly suggest the involvement of enteroviruses, such as coxsackievirus b, in the development of t1dm. enteroviral rna and/or proteins can be detected in tissues of patients with t1dm. isolation of coxsackievirus b4 from t ... | 2010 | 20351698 |
| etiology of bronchiolitis in a hospitalized pediatric population: prospective multicenter study. | in 2006, bronchiolitis due to adenovirus nosocomial infections resulted in the closure of a pediatric department in northern portugal. | 2010 | 20362492 |
| postinfectious and chronic fatigue syndromes: clinical experience from a tertiary-referral centre in norway. | we aimed to compare patients reporting acute infection with those reporting no infection at onset of chronic fatigue syndrome (cfs). | 2010 | 20363992 |
| an outbreak of aseptic meningitis caused by coxsackievirus a9 in gansu, the people's republic of china. | background: an outbreak of aseptic meningitis occurred in tianshui city of gansu province, the people's republic of china, from march to june 2005. a total of 85 patients were clinical confirmed as aseptic meningitis in this outbreak. results: cva9 was mainly responsible for this outbreak supported by the clinical manifestations of the patients, epidemiological data of the outbreak, the results of rt-pcr and complete vp1 sequence determination, conventional neutralization assays, igm serological ... | 2010 | 20367886 |
| detection of poliovirus-infected macrophages in thymus of patients with myasthenia gravis. | genetic and environmental factors are thought to contribute to the etiology of the autoimmune disease myasthenia gravis (mg). viral involvement has long been suspected, but direct evidence of involvement has not been found. we recently reported that toll-like receptor 4 (tlr4)-a key activator of innate immunity-was overexpressed in the thymus of some patients with mg, suggesting that thymic infection by pathogens might be involved in mg pathogenesis. we searched for evidence of intrathymic infec ... | 2010 | 20368632 |
| molecular characterization of echovirus 30-associated outbreak of aseptic meningitis in korea in 2008. | evaluation of the primary etiologic agents that cause aseptic meningitis outbreaks may provide valuable information regarding the prevention and management of aseptic meningitis. in korea, an outbreak of aseptic meningitis caused by echovirus type 30 (e30) occurred from may to october in 2008. in order to determine the etiologic agent, csf and/or stool specimens from 140 children hospitalized for aseptic meningitis at soonchunhyang university cheonan hospital between june and october of 2008 wer ... | 2010 | 20372040 |
| a single coxsackievirus b2 capsid residue controls cytolysis and apoptosis in rhabdomyosarcoma cells. | coxsackievirus b2 (cvb2), one of six human pathogens of the group b coxsackieviruses within the enterovirus genus of picornaviridae, causes a wide spectrum of human diseases ranging from mild upper respiratory illnesses to myocarditis and meningitis. the cvb2 prototype strain ohio-1 (cvb2o) was originally isolated from a patient with summer grippe in the 1950s. later on, cvb2o was adapted to cytolytic replication in rhabdomyosarcoma (rd) cells. here, we present analyses of the correlation betwee ... | 2010 | 20375176 |
| evolutionary trajectory of the vp1 gene of human enterovirus 71 genogroup b and c viruses. | from 1963 to 1986, human enterovirus 71 (hev71) infections in the netherlands were successively caused by viruses of subgenogroups b0, b1 and b2. a genogroup shift occurred in 1987, after which viruses of subgenogroups c1 and c2 were detected exclusively. this is in line with hev71 typing in australia, europe and the usa, but is distinct from that in the asian pacific region, where hev71 subgenogroups b3-b5 and c4-c5 have caused large outbreaks since 1997. to understand these observations in hev ... | 2010 | 20375223 |
| knowledge about and attitude toward enterovirus 71 infections: a survey of parents and teachers at kindergartens in taiwan. | in 2008, we surveyed 690 parents and 104 teachers at kindergartens in taiwan about their knowledge of and attitude towards enterovirus 71 infections. the accurate response rate for enterovirus infection characteristics was greater than 80% for specific symptoms, prevalent age group, and predominant infection season. parents and teachers felt great anxiety and even panic about infection. both groups perceived the impact of enterovirus infection to be worse than that of influenza. | 2010 | 20227791 |
| myeloid differentiation factor-88 contributes to tlr9-mediated modulation of acute coxsackievirus b3-induced myocarditis in vivo. | toll-like receptor 9 (tlr9) is a member of the innate immune system and has been shown to influence myocardial function, but its role in myocarditis is hitherto unknown. we therefore investigated whether or not tlr9 plays a role in this disease in coxsackievirus b3 (cvb3)-induced myocarditis in mice. left ventricular (lv) function, cardiac immune cell infiltration, virus mrna, and components of the tlr9 downstream pathway were investigated in tlr9-deficient [knockout (ko)] and wild-type (wt) mic ... | 2010 | 20228254 |
| [immune effects of four coxsackievirus b3 vp1 dna fusion vaccines in mice]. | to compare the immunogenicity and protective effects on cvb3 infected mice of four dna fusion vaccines coupling coxsackievirus b3 (cvb3) vp1 with macrophage-derived chemokine (mdc), c3d3, shiga toxin b subuit (stxb) and mouse beta-defensin-2 (mbd2), respectively. | 2010 | 20230664 |
| environmental surveillance and molecular characterization of human enteric viruses in tropical urban wastewaters. | to study the prevalence and genotypes of waterborne pathogenic viruses in urban wastewaters in the tropical region. | 2010 | 20233263 |
| early days of food and environmental virology. | in july 1962, the author joined the food research institute (fri), then at the university of chicago, to become its food virologist. there was a limited record of waterborne viral disease outbreaks at the time; recorded data on foodborne outbreaks were fewer still. laboratory environmental (water and wastewater) virology was in its infancy, and food virology was in gestation. detection of viruses was most often attempted by inoculation of primary primate cell cultures, with observation for plaqu ... | 2010 | 20234839 |
| viral meningoencephalitis: a review of diagnostic methods and guidelines for management. | viral encephalitis is a medical emergency. the prognosis depends mainly on the pathogen and host immunologic state. correct immediate diagnosis and introduction of symptomatic and specific therapy has a dramatic influence upon survival and reduces the extent of permanent brain injury. | 2010 | 20236175 |
| removal of human enteric viruses and indicator microorganisms from domestic wastewater by aerated lagoons. | aerated lagoons offer a low-cost and simple approach to treating domestic wastewater in small municipalities. the objective of the current study was to evaluate, for each cell in the lagoons, the removal of indicator microorganisms and human enteric viruses under warm (summer) and cold (early spring) conditions. the two sites are located in southwest quebec, canada. samples were assayed for thermotolerant coliforms, enterococci, clostridium perfringens, somatic and male-specific coliphages, and ... | 2010 | 20237581 |
| transcriptome profiling of host-microbe interactions by differential display rt-pcr. | in recent years, dna microarray has become increasingly popular as a tool to investigate global expression patterns compared to differential display rt-pcr. although differential display rt-pcr can be labour-intensive, it has its own merits over those of dna microarray. while the latter usually consists of a well-defined set of species-specific genes, differential display rt-pcr allows the investigation of host-microbe interactions without bias towards any mrna transcripts. this means that the r ... | 2010 | 20300989 |
| ev71: an emerging infectious disease vaccine target in the far east? | hand, foot, and mouth disease (hfmd) is a common viral illness in infants and children caused by viruses that belong to the enterovirus genus of the picornavirus family. although most hfmd do not result in serious complications, outbreaks of hfmd caused by enterovirus 71 (ev71) can present with a high rate of neurological complications, including meningoencephalitis, pulmonary complications, and possibly death. hfmd caused by ev71 has become a major emerging infectious disease in asia and the hi ... | 2010 | 20304038 |
| genetic analysis of the vp1 region of enterovirus 71 reveals the emergence of genotype a in central china in 2008. | enterovirus 71 (ev71) strains from children were characterized by full-length vp1 nucleotide sequencing. out of 22 clinical specimens, five isolates identified as ev71 were recovered by virus isolation. the vp1 sequences of the five isolates had more than 97.4% sequence identity with prototype virus brcr, clustering in the genotype a lineage. this represents the first record of genotype a ev71 in china since the brcr prototype strain was discovered in the usa in 1969. | 2010 | 20306124 |
| fulminant myocarditis with dramatic response to corticoids. | a 47-year-old healthy man developed acute fulminant coxsackie bi myocarditis with cardiogenic shock and extreme elevation of nt-pro-bnp (82585 pg/ml). he had a dramatic and rapid improvement within the following days after administration of corticoids on top of dobutamine and classic treatment. | 2010 | 20306899 |
| islet inflammation and cxcl10 in recent-onset type 1 diabetes. | type 1 diabetes results from a t cell-mediated destruction of insulin-producing pancreatic beta cells. little is known on local factors contributing to migration of t cells to pancreatic tissue. we recently demonstrated evidence of viral infection in beta cells in several recent-onset type 1 diabetes patients. islet inflammation was analysed in a series of new- or recent-onset type 1 diabetic patients and non-diabetic control subjects. autoimmune t cell reactivity was studied in lymphocytes deri ... | 2010 | 20059481 |
| analysis of recombination and natural selection in human enterovirus 71. | the development of effective vaccines and antiviral prophylaxis against human enterovirus 71 (ev71) has been hampered by the extensive antigenic diversity of the virus. to gain new insights into the evolutionary processes that create this genetic diversity, the treeorder scan method and rdp program were employed to detect recombination events in the genome, and then parsimony-based and maximum-likelihood-based methods were used to detect natural selection effects on viral proteins. recombination ... | 2010 | 20060995 |
| critical issues related to porcine xenograft exposure to human viruses: lessons from allotransplantation. | xenotransplantation of tissues from swine into humans poses the threat of bidirectional transfer of porcine or human microorganisms to the recipient or to the xenograft, respectively. this review focuses on recipient-derived infection. recent data are reviewed that assess the susceptibility of porcine cells to human viruses. on the basis of the experience in allotransplantation, potential consequences for the xenograft are discussed. | 2010 | 20061950 |
| enteroviruses, type 1 diabetes and hygiene: a complex relationship. | type 1 diabetes (t1d) is an autoimmune disease in which the immune system mounts an attack on the host's insulin-producing beta cells. because most cases of t1d cannot be attributed only to individual genetics, it is strongly inferred that there is a significant environmental contribution, such as infection, impacting disease development. the human enteroviruses (hev) are common picornaviruses often implicated as triggers of human t1d, although precisely which of the numerous hev may be involved ... | 2010 | 20049905 |
| human enterovirus 71 and hand, foot and mouth disease. | hand, foot and mouth disease (hfmd) is generally a benign febrile exanthematous childhood disease caused by human enteroviruses. the route of transmission is postulated to be faeco-oral in developing areas but attributed more to respiratory droplet in developed areas. transmission is facilitated by the prolonged environmental survival of these viruses and their greater resistance to biocides. serious outbreaks with neurological and cardiopulmonary complications caused by human enterovirus 71 (he ... | 2010 | 20056019 |
| in vitro anti-enterovirus 71 activity of gallic acid from woodfordia fruticosa flowers. | the anti-enterovirus 71 (ev71) activity of six nepalese plants' extracts and gallic acid (ga) isolated from woodfordia fruticosa kurz (family; lythaceae) flowers were evaluated in vero cells. | 2010 | 20149083 |
| can human viruses infect porcine xenografts? | xenotransplantation exposes the recipient to known and unknown pathogens of the donor pig (donor-derived xenosis). a major effort has been undertaken to minimize the risk of transmission from the donor using specialized breeding techniques. with the exception of endogenous retroviruses and porcine lymphotropic herpesvirus, exclusion of known pathogens was successful and has eliminated a majority of donor pathogens. in the recipient, enhanced replication of many pathogens will be stimulated by th ... | 2010 | 20149184 |
| [consensus conference on acute bronchiolitis (ii): epidemiology of acute bronchiolitis. review of the scientific evidence]. | a review of the evidence on epidemiology, risk factors, etiology and clinical-etiological profile of acute bronchiolitis is presented. the frequency estimates are very heterogeneous; in the population under two years the frequency of admission for bronchiolitis is between 1 and 3.5%, primary care consultations between 4 and 20% and emergency visits between 1 and 2%. the frequency of admissions for respiratory infection by respiratory syncytial virus in the risk population is: in premature infant ... | 2010 | 20153707 |
| detection and characterization of human parechoviruses in archived cell cultures, in hungary. | human parechoviruses (hpev) belonging to the family picornaviridae are widespread enteric pathogens and associated with various clinical symptoms in humans. | 2010 | 20153974 |
| molecular identification and analysis of nonserotypeable human enteroviruses. | conventional approaches to characterizing human enteroviruses (hevs) are based on viral isolation and neutralization. molecular typing methods depend largely on reverse transcription-pcr (rt-pcr) and nucleotide sequencing of the entire or partial vp1 gene. a modified rt-pcr-based reverse line blot (rlb) hybridization assay was developed as a rapid and efficient way to characterize common and nonserotypeable (by neutralization) hevs. twenty hev serotypes accounted for 87.1% of all hevs isolated a ... | 2010 | 20164278 |
| a comparison of the vp1, vp2, and vp4 regions for molecular typing of human enteroviruses. | the vp4, vp2, and vp1 gene regions were evaluated for their usefulness in typing human enteroviruses. three published rt-pcr primers sets targeting separately these three gene regions were used. initially, from a total of 86 field isolates (36 hev-a, 40 hev-b, and 10 hev-c) tested, 100% concordance in hev-a was identified from all three gene regions (vp4, vp2, and vp1). however, for hev-b and hev-c viruses, only the vp2 and vp1 regions, and not vp4, showed 100% concordance in typing these viruse ... | 2010 | 20166171 |
| the cross-reactivity of the enterovirus 71 to human brain tissue and identification of the cross-reactivity related fragments. | ev71 occasionally cause a series of severe neurological symptoms, including aseptic meningitis, encephalitis, and poliomyelitis-like paralysis. however, the neurological destruction mechanism was remained to be clarified. this study described the cross reaction between ev71 induced igg and human brain tissue. | 2010 | 20170551 |
| european homograft bank: twenty years of cardiovascular tissue banking and collaboration with transplant coordination in europe. | established in 1989 in brussels as an international nonprofit association, the european homograft bank (ehb) has been collaborating closely with the transplant coordination of the different centers in belgium and other european countries. donor selection is made after discussion of exclusion criteria with the transplant coordinator of the procurement center. ehb collaborates with 15 belgian, 11 german, 10 french, 10 swiss, 3 italian, 3 dutch, and some other procurement and/or implantation center ... | 2010 | 20172310 |
| [laboratory diagnosis of lymphocytic meningitis]. | lymphocytic meningitis, mainly those with an acute and benign course, are caused by viruses. in our area, the most commonly involved agents are enteroviruses, herpes simplex, varicella zoster and toscana viruses. nucleic acids amplification techniques (naat) are the methods of choice to diagnose viral meningitis from cerebrospinal fluid (csf) samples. they are more rapid and sensitive, and indeed, they are not influenced by the viability of the virus in the clinical specimen as traditional metho ... | 2010 | 20172425 |
| pyrazole compound bpr1p0034 with potent and selective anti-influenza virus activity. | influenza viruses are a major cause of morbidity and mortality around the world. more recently, a swine-origin influenza a (h1n1) virus that is spreading via human-to-human transmission has become a serious public concern. although vaccination is the primary strategy for preventing infections, influenza antiviral drugs play an important role in a comprehensive approach to controlling illness and transmission. in addition, a search for influenza-inhibiting drugs is particularly important in the f ... | 2010 | 20178582 |
| selenium level surveillance for the year 2007 of keshan disease in endemic areas and analysis on surveillance results between 2003 and 2007. | selenium deficiency is widely accepted as a fundamental cause of keshan disease (kd). in the present study, the selenium levels of hair and food samples of kd in some endemic areas were measured; the prevalence and incidence of kd for the year 2007 in these areas were surveyed. the results for kd surveillance from 2003 to 2007 were analyzed. the selenium level was measured fluorometrically. in 2007, a total of 19,280 residents were surveyed in 15 provinces. the prevalence and incidence of kd wer ... | 2010 | 20180046 |
| the role of infection in carotid plaque pathogenesis and stability: the clinical evidence. | chlamydia pneumoniae was the first pathogen linked with carotid atherosclerotic changes and plaque rupture. currently, other common pathogens are also under investigation as potential contributors. | 2010 | 20180770 |
| evaluation of adenovirus and e. coli as indicators for human enteric viruses presence in mussels produced in la spezia gulf (italy). | the purpose of this work was to verify whether e. coli is a good indicator of viral contamination in mussels and adenovirus could represent a better alternative as indicator organism of viral presence to guarantee consumer health protection. | 2010 | 20184672 |
| co-circulation of coxsackieviruses a6 and a10 in hand, foot and mouth disease outbreak in finland. | a nationwide outbreak of hand, foot and mouth disease (hfmd) occurred in finland in autumn 2008. the outbreak was untypical since a considerable number of clinically diagnosed patients were adults. furthermore, many of the patients suffered from onychomadesis several weeks after the acute phase of hfmd. | 2010 | 20189452 |
| characterization of anti-coxsackie virus b3 constituents of radix astragali by high-performance liquid chromatography coupled with electrospray ionization tandem mass spectrometry. | to profile the anti-coxsackie virus b3 constituents of radix astragali, an hplc-dad-ms(n) analytical method, combined with an in vivo test, has been developed to identify the constituents of the active part, which has been demonstrated to have potency to inhibit the proliferation of virus in cardiac muscle, alleviate infraction in heart and elevate the survival rate of the animal. by comparing their retention time and ms data with those obtained from the authentic compounds and the published dat ... | 2010 | 20120039 |
| tumor necrosis factor-alpha promotes myocarditis in female mice infected with coxsackievirus b3 through upregulation of cd1d on hematopoietic cells. | coxsackievirus b3 (cvb3) induces cardiac inflammation (myocarditis) in male but not female c57bl/6 mice. protection of females correlates with reduced expression of tnf-alpha and il-1beta at both the mrna and protein levels in the heart. treatment of females with 300 ng/mouse of recombinant tnf-alpha on days +1 and +3 relative to infection restores myocarditis susceptibility to levels approximating those of infected male mice, showing that tnf-alpha deficiency is central to disease resistance. f ... | 2010 | 20121405 |
| detection of enterovirus rna in peripheral blood mononuclear cells of type 1 diabetic patients beyond the stage of acute infection. | previous studies have shown that enteroviral rna can be detected in blood at the onset of type 1 diabetes (t1d). the infection may play a role in triggering t1d and genetic host factors may contribute to this process. we investigated (1) whether enterovirus is present at the onset of t1d in peripheral blood mononuclear cells (pbmc), plasma, throat, or stool, and (2) whether enteroviral presence is linked with hla-dr type and/or polymorphisms in melanoma differentiation-associated gene 5 (mda5) a ... | 2010 | 20121407 |
| newly identified human rhinoviruses: molecular methods heat up the cold viruses. | human rhinovirus (hrv) infections cause at least 70% of virus-related wheezing exacerbations and cold and flu-like illnesses. they are associated with otitis media, sinusitis and pneumonia. annually, the economic impact of hrv infections costs billions in healthcare and lost productivity. since 1987, 100 officially recognised hrv serotypes reside in two genetically distinct species; hrv a and hrv b, within the genus enterovirus, family picornaviridae. sequencing of their approximately 7kb genome ... | 2010 | 20127751 |