Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| annual variation of complement, lysozyme and haemagglutinin levels in serum of the gilthead sea bream sparus aurata. | 2003 | 14550674 | |
| life cycle of ceratothoa oestroides, a cymothoid isopod parasite from sea bass dicentrarchus labrax and sea bream sparus aurata. | ceratothoa oestroides (risso, 1826) (isopoda: cymothoida) is a protandric hermaphrodite parasite on a wide range of wild fish species. in recent years it has become a threat to cage-reared fish facilities, where high fish density provides optimal conditions for transmission. its impact on fish health and economical gain is significant, varying from growth retardation and decreased immunocompetency to direct loss due to mass mortalities of juvenile fishes. because of the sheltered location of the ... | 2003 | 14735926 |
| growth hormone expression in ontogenic development in gilthead sea bream. | the pattern of expression of the growth hormone (gh) gene was studied during the early development of gilthead sea bream ( sparus aurata). the gh transcript was detected from the 2nd day of the larval stage onwards. in the next stages the expression level fluctuated, possibly due to different regulatory factors. the distribution of gh mrna studied by in situ hybridization (ish) was found to be pituitary specific. hybridization signals for gh mrna were detected for the first time in 4-day-old lar ... | 2003 | 12827495 |
| acclimation of s aurata to various salinities alters energy metabolism of osmoregulatory and nonosmoregulatory organs. | the impact of different environmental salinities on the energy metabolism of gills, kidney, liver, and brain was assessed in gilthead sea bream (sparus aurata) acclimated to brackish water [bw, 12 parts/thousand (ppt)], seawater (sw, 38 ppt) and hyper saline water (hsw, 55 ppt) for 14 days. plasma osmolality and levels of sodium and chloride presented a clear direct relationship with environmental salinities. a general activation of energy metabolism was observed under different osmotic conditio ... | 2003 | 12816745 |
| developmental ontogeny of prolactin and prolactin receptor in the sea bream (sparus aurata). | the expression of prl and its receptor (prlr) were characterised during sea bream embryonic and larval development, by semi-quantitative and quantitative rt-pcr, respectively, until 46 days post-hatch (dph). immunocytochemistry with antisera specific for sea bream prlr was carried out with larval sections from hatching up to 46 dph. a single transcript of prl (1.35 kb) and prlr (2.8 kb) identical to the transcripts previously characterised in adult tissue, are present in sea bream embryos and la ... | 2003 | 12812779 |
| dna damage induced by copper on erythrocytes of gilthead sea bream sparus aurata and mollusk scapharca inaequivalvis. | heavy metal pollution in rivers and its impact on aquatic ecosystems is a dynamic process. fish are ideal indicators of heavy metal contamination in aquatic systems because they occupy different trophic levels and are of different sizes and ages. in particular, copper is an essential trace metal for living organisms and it is present in all natural waters and sediments. in this paper, we report data on the effect of copper on dna erythrocytes from the teleost gilthead sea bream sparus aurata and ... | 2003 | 14674588 |
| isolation of sparus auratus prolactin gene and activity of the cis-acting regulatory elements. | a sea bream prolactin (sbprl) gene was isolated using a prolactin cdna fragment, generated by pcr as a probe. the gene analyzed comprises 3.5 kb of dna containing five exons as described previously for other fish prl genes. analysis of 1.0 kb of the proximal promoter sequence reveals a consensus tataa box, up to seven (a/t)3ncat consensus motifs for binding of the pituitary-specific factor pit-1 and putative creb and gata binding sites. cho culture cells co-transfected with a sbprl promoter sequ ... | 2003 | 13129503 |
| thynnascaris rhacodes sp. n. (nematoda: ascaridoidea) in fishes from the israeli mediterranean coast. | thynnascaris rhacodes sp. n. infects pelates quadrilineatus, solea vulgaris, boops boops, lithognathus mormyrus, obleida melanura, diplodus vulgaris, d. sargus, and sparus auratus from the eastern mediterranean sea. unique in that the cuticle on the female tail is wrinkled, the ascaridoid is further characterized by possessing 27 to 35 precloacal papillae, a body length of 11 to 85 mm, similar spicules 3 to 6% of body length, a length-ratio of intestinal cecum to ventricular appendage of 1 : 1 t ... | 2003 | 570370 |
| molecular cloning and characterization of gilthead sea bream (sparus aurata) growth hormone receptor (ghr). assessment of alternative splicing. | the full-length growth hormone receptor (ghr) of gilthead sea bream (sparus aurata) was cloned and sequenced by rt-pcr and rapid amplification of 5'and 3'ends. the open reading frame codes for a mature 609 amino acid protein with a hydrophobic transmembrane region and all the characteristic motifs of ghrs. sequence analysis revealed a 96 and 76% of amino acid identity with black sea bream (acanthopagrus schlegeli) and turbot (scophthalmus maximus) ghrs, respectively, but this amino acid identity ... | 2003 | 12941635 |
| temperature and the expression of seven muscle-specific protein genes during embryogenesis in the atlantic cod gadus morhua l. | seven cdna clones coding for different muscle-specific proteins (msps) were isolated from the fast muscle tissue of atlantic cod gadus morhua l. in situ hybridization using crna probes was used to characterize the temporal and spatial patterns of gene expression with respect to somite stage in embryos incubated at 4 degrees c, 7 degrees c and 10 degrees c. myod transcripts were first observed in the presomitic mesoderm prior to somite formation, and in the lateral compartment of the forming somi ... | 2003 | 12909700 |
| protein and arginine requirements for maintenance and nitrogen gain in four teleosts. | besides being an indispensable amino acid for protein synthesis, arginine (arg) is also involved in a number of other physiological functions. available data on the quantitative requirement for arg in different teleosts appear to show much variability. so far, there are very limited data on the maintenance requirements of indispensable amino acids (iaa) in fish. in the present study, we compared n and arg requirements for maintenance and growth of four finfish species: rainbow trout (oncorhynchu ... | 2002 | 12010584 |
| gluconeogenic enzyme gene expression is decreased by dietary carbohydrates in common carp (cyprinus carpio) and gilthead seabream (sparus aurata). | our objective is to understand the low metabolic utilization of dietary carbohydrates in fish. we compared the regulation of gluconeogenic enzymes at a molecular level in two fish species, the common carp (cyprinus carpio) and gilthead seabream (sparus aurata), known to be relatively tolerant to dietary carbohydrates. after cloning of partial cdna sequences for three key gluconeogenic enzymes (glucose-6-phosphatase (g6pase), fructose biphosphatase (fbpase) and phosphoenolpyruvate carboxykinase ( ... | 2002 | 12401217 |
| nutritional regulation of glucose-6-phosphatase gene expression in liver of the gilthead sea bream (sparus aurata). | to examine the role of glucose-6-phosphatase (g6pase) in glucose homeostasis in the diabetes-like experimental model of carnivorous fish, we analysed postprandial variations and the effect of starvation, ration size and diet composition on the regulation of g6pase expression at the enzyme activity and mrna level in the liver of gilthead sea bream (sparus aurata). g6pase expression increased in long-term starved or energy-restricted fish. in contrast to data reported for other fish species, short ... | 2002 | 12493082 |
| endocrine mediators of seasonal growth in gilthead sea bream (sparus aurata): the growth hormone and somatolactin paradigm. | regulation of somatolactin (sl) and the somatotropic axis was examined year-around at three different stocking times (spring, summer, and autumn) in a mediterranean fish, the gilthead sea bream (sparus aurata). the overall timing of plasma growth hormone (gh) increase was similar among trials (late spring-early summer), but the range of variation year-around was different and followed changes in food intake. total plasma insulin-like growth factor-i primarily followed changes on growth rates, an ... | 2002 | 12392683 |
| co-expression of somatolactin mrnas in the pituitary gland of gilthead sea bream. | the expression of mrnas of the two types of somatolactin which have been found, up to the present, in the pituitary of gilthead sea bream (sparus aurata) have been analyzed by in situ hybridization (ish). the method of non-radioactive ish, which we optimize in this study, uses oligonucleotides labelled in the 5' end with biotin or dioxygenin as probes. this allows simple and double ish of high specificity and sensitivity to be performed. the distinct somatolactin oligoprobes used present a stron ... | 2002 | 12382144 |
| calcium balance in sea bream (sparus aurata): the effect of oestradiol-17beta. | in all teleost fishes vitellogenesis is triggered and maintained by oestradiol-17beta (e2) and is accompanied by an increase of blood plasma calcium and phosphate. the action of this hormone on calcium metabolism was investigated by treating fast-growing immature juvenile sea bream (sparus aurata) with coconut butter implants alone (control) or implants containing 10 microg/g e2. treatment with e2 induced the production of circulating vitellogenin, a 2.5-fold increase in plasma ionic ca2+ and a ... | 2002 | 12010645 |
| comparative quality assessment of cultured and wild sea bream (sparus aurata) stored in ice. | comparative quality assessment of cultured and wild sea bream stored in ice for up to 23 days was achieved by the monitoring of sensory quality, levels of nucleotide, nucleotide breakdown products, and texture by a texturometer. the changes in sensory quality of both raw and cooked fish were assessed using the modified tasmanian and torry schemes, respectively. k and related values (freshness indicators), namely, k, k(i), g, p, h, and f(r), were calculated. linear increases (r(2) > or = 0.99) in ... | 2002 | 11902954 |
| hormonal mechanisms regulating hepatic vitellogenin synthesis in the gilthead sea bream, sparus aurata. | experiments were carried out to study in vitro the effects of 17beta-estradiol (e(2)), homologous pituitary homogenate (hph), and recombinant red sea bream growth hormone (sbgh) on vitellogenin (vtg) secretion from cultured sea bream liver fragments. basal secretion of vtg was found to be significantly higher in the prespawning period, compared with sea bream liver in the spawning and postspawning periods. similarly, the sea bream liver obtained during the prespawning period responded more signi ... | 2002 | 12176724 |
| cyclic adpr and calcium signaling in sea bream (sparus aurata) egg fertilization. | the cell egg is in a state of quiescence and only after its fusion with the sperm, a series of pre-programmed metabolic processes will be activated, culminating with embryonic development. the egg/sperm fusion induces a transitory increase of ca(2+) in the cytoplasm, which is responsible for the activation of both precocious and late reactions. the release of ca(2+) occurs by stimulation of the ionic specific channels. in addition to ip(3), a new ca-release inducer was recently evidenced, cyclic ... | 2002 | 11803557 |
| formation of short-lived multinucleated giant cells (mgcs) from cultured gilthead seabream macrophages. | monocytes/macrophages obtained from the head kidney and peritoneal cavity of gilthead seabream (sparus aurata) were cultured using plates from three different manufacturers, and were maintained under different conditions. the effects on the morphology and fusion of monocytes/macrophages of initial cell loading, removal of non-adherent cells at different times after plating, and addition of serum and antibiotics were evaluated by light microscopy, and transmission (tem) and scanning (sem) electro ... | 2002 | 12115269 |
| pcr detection and pfge dna macrorestriction analyses of clinical isolates of pseudomonas anguilliseptica from winter disease outbreaks in sea bream sparus aurata. | a pcr-based detection system for pseudomonas anguilliseptica was evaluated. the primer combination paf-par (forward primer paf = 5'-gacctcgccatta-3', reverse primer par = 5'-ctcagcagttttgaaag-3') gave a unique and specific amplification product of 439 bp at an annealing temperature of 46 degrees c with all the p. anguilliseptica isolates and strains (n = 56) but no amplification products were observed with any other pseudomonas species or phylogenetically related bacteria tested. the pcr assay h ... | 2002 | 12152901 |
| cryptosporidium molnari n. sp. (apicomplexa: cryptosporidiidae) infecting two marine fish species, sparus aurata l. and dicentrarchus labrax l. | cryptosporidium molnari n. sp. is described from two teleost fish, the gilthead sea bream (sparus aurata l.) and the european sea bass (dicentrarchus labrax l.). the parasite was found mainly in the stomach epithelium and seldom in the intestine. oocysts were almost spherical, with four naked sporozoites and a prominent residuum, and measured 3.23-5.45 x 3.02-5.04 (mean 4.72 x 4.47) microm in the type host, gilthead sea bream (shape index 1-1.17, mean 1.05). sporulation was endogenous, as fully ... | 2002 | 12076630 |
| physiological changes in the spawning gilthead seabream, sparus aurata, succeeding the removal of males. | the physiological effects triggered in females by the removal of males from a group of spawning fish were examined in the multiple batch spawner, the gilthead seabream, sparus aurata. one week after the removal of males, a large portion of the oocytes underwent atresia, and sporadic release of low quality eggs continued at low frequency over a period of seven weeks. the transcript levels of the three native gonadotropin releasing hormone (gnrh) forms, salmon (s)gnrh, seabream (sb)gnrh, and chick ... | 2002 | 12115938 |
| ontogeny, tissue distribution, and hormonal regulation of insulin-like growth factor binding protein-2 (igfbp-2) in a marine fish, sparus aurata. | in this study, we have cloned insulin-like growth factor binding protein (igfbp)-2 from a marine hermaphroditic fish species, the gilthead sea bream (sparus aurata), and determined its structure, ontogeny, tissue distribution, and hormonal regulation. the sea bream igfbp-2 precursor consists of 286 amino acids, including a putative signal peptide of 22 residues and a mature protein of 264 residues. the overall sequence of sea bream preigfbp-2 is 52-39% identical to that of zebrafish, chick, mous ... | 2002 | 12392684 |
| osmoregulatory action of prl, gh, and cortisol in the gilthead seabream (sparus aurata l). | the osmoregulatory actions of ovine prolactin (oprl), ovine growth hormone (ogh), and cortisol were tested in the euryhaline gilthead seabream sparus aurata. acclimated to sea water (sw, 40 ppt salinity, 1000 mosm/kg h(2)o) or brackish water (bw, 5 ppt, salinity, 130 mosm/kg h(2)o), injected every other day for one week (number of injections, 4) with saline (0.9% nacl), oprl (4 microg/g body weight), ogh (4 microg/g body weight) or cortisol (5 microg/g body weight), and transferred from sw to bw ... | 2002 | 12441119 |
| immunocytochemical demonstration of melanotropic and adrenocorticotropic cells from the gilthead sea bream (sparus aurata l., teleostei) by light and electron microscopy: an ontogenic study. | in the pituitary of gilthead sea bream, sparus aurata, melanotropic (msh) and adrenocorticotropic (acth) cells were identified at the light and electron microscopic levels using rabbit anti-synthetic alphamsh (msh) and anti-human acth (1-24) (acth) sera. the distribution of these cell types was followed from hatching to 48 months. the techniques used included the peroxidase anti-peroxidase (pap) method, conventional electron microscopy, and an immunogold technique. using pap, msh (immunoreactive ... | 2002 | 11884085 |
| olfactory sensitivity to changes in environmental [ca(2+)] in the freshwater teleost carassius auratus: an olfactory role for the ca(2+)-sensing receptor? | olfactory sensitivity to changes in environmental ca(2+) has been demonstrated in two teleost species; a salmonid (oncorhynchus nerka) and a marine/estuarine perciform (sparus aurata). to assess whether this phenomenon is restricted to species that normally experience large fluctuations in external ion concentrations (e.g. moving from sea water to fresh water) or is present in a much wider range of species, we investigated olfactory ca(2+) sensitivity in the goldfish (carassius auratus), which i ... | 2002 | 12177141 |
| amplified fragment length polymorphism (aflp) and biochemical typing of photobacterium damselae subsp. damselae. | the aim of the present study was to characterize subspecifically photobacterium damselae subsp. damselae strains isolated from cultured sparus aurata and dicentrarchus labrax by means of phenotypic and molecular typing techniques (amplified fragment length polymorphism, aflp). | 2002 | 12234352 |
| amoeba-like infections in cultured marine fishes: systemic infection in pompano trachinotus falcatus l. from singapore and gill disease associated with paramoeba sp. in sea bream sparus aurata l. from greece. | two cases of amoeba-like infections in cultured warmwater marine fish are described, an unusual systemic infection in pompano trachinotus falcatus l. from singapore and a gill infection in mediterranean sea bream sparus aurata l. all pompano showed marked systemic infection of amoeba-like parasites in gills, kidney, intestine, pancreas and spleen. the most severe lesions were in the gills and renal tissue with minimal tissue reaction in other organs. | 2002 | 12449253 |
| identification of cadmium-sensitive genes in the antarctic fish chionodraco hamatus by messenger rna differential display. | to investigate the ability of cadmium to affect gene transcription in fish, the messenger rna (mrna) differential display technique was used to analyze gene expression in the antarctic icefish chionodraco hamatus exposed to sublethal doses of cadmium salt. seven dna complementary to rna (cdna) bands whose steady-state levels of expression significantly changed in response to cadmium exposure were identified. the results obtained show that two groups of genes are affected by cadmium in icefish li ... | 2002 | 12459259 |
| characterization of pseudomonas spp. associated with spoilage of gilt-head sea bream stored under various conditions. | the population dynamics of pseudomonads in gilt-head sea bream mediterranean fish (sparus aurata) stored under different conditions were studied. phenotypic analysis and sodium dodecyl sulfate-polyacrylamide gel electrophoresis of whole-cell proteins were performed to identify a total of 106 pseudomonas strains isolated from s. aurata stored under different temperatures (at 0, 10, and 20 degrees c) and packaging conditions (air and a modified atmosphere of 40% co(2)-30% n(2)-30% o(2)). pseudomon ... | 2002 | 11772610 |
| the gnrh system in the european sea bass (dicentrarchus labrax). | the cdna sequences encoding three gnrh forms, sea bream gnrh (sbgnrh), salmon gnrh (sgnrh) and chicken gnrh ii (cgnrh ii), were cloned from the brain of european sea bass, dicentrarchus labrax. comparison of their deduced amino acid sequences to the same forms in the gilthead sea bream, sparus aurata, and striped bass, morone saxatilis, revealed high homology of the prepro-cgnrh ii (94% and 98% respectively), and prepro-sgnrh (92% to both species). the sbgnrh exhibited dissimilar identities, wit ... | 2002 | 11786378 |
| cloning of the cdna for the putative calcium-sensing receptor and its tissue distribution in sea bream (sparus aurata). | the cdna for the calcium-sensing receptor (casr) gene has been cloned from the marine teleost sparus aurata, the sea bream. the isolated clones were 3.3 kb long with an open reading frame of 2820 bp, a 5' utr of 240 bp, and 3' utr of 248 bp. the gene codes for a mature peptide of 940 amino acids which has three principal domains; the extracellular region is more than half the total protein, there is a seven-transmembrane domain, and there is a short intracellular domain. there is considerable se ... | 2002 | 12383439 |
| photobacterium damselae ssp. piscicida: detection by direct amplification of 16s rrna gene sequences and genotypic variation as determined by amplified fragment length polymorphism (aflp). | a pcr protocol for the rapid diagnosis of fish 'pasteurellosis' based on 16s rrna gene sequences was developed. the procedure combines low annealing temperature that detects low titers of photobacterium damselae but also related species, and high annealing temperature for the specific identification of p. damselae directly from infected fish. the pcr protocol was validated on 19 piscine isolates of p. damselae ssp. piscicida from different geographic regions (japan, italy, spain, greece and isra ... | 2002 | 12033705 |
| oral administration of yeast, saccharomyces cerevisiae, enhances the cellular innate immune response of gilthead seabream (sparus aurata l.). | the effects of including lyophilised whole yeast, saccharomyces cerevisiae, in the diet on the seabream innate immune response were investigated. gilthead seabream (sparus aurata l.) specimens were fed four different diets for 4 weeks: a commercial diet as control and the same diet supplemented with 1, 5 or 10 g/kg yeast. after 1, 2 and 4 weeks, serum complement titres, as a humoral parameter, and phagocytic, respiratory burst, myeloperoxidase and natural cytotoxic activities of head-kidney leuc ... | 2002 | 11867166 |
| parathyroid hormone-related protein in lower vertebrates. | the genes for parathyroid hormone-related protein (pthrp) have been cloned in two teleost fishes, cdna of sea bream (sparus aurata) and genomic dna of puffer fish (fugu rubripes). the gene sequences show that there is significant conservation of amino acid identity, with specific domains most highly conserved. the n-terminus, responsible for bone matrix lysis in mammals and chickens, is present in the fish genes with 52% sequence identity to higher vertebrate pthrp peptides; the nuclear transpor ... | 2002 | 11997212 |
| molecular cloning and expression analysis of tumor necrosis factor alpha from a marine fish reveal its constitutive expression and ubiquitous nature. | the tumor necrosis factor alpha ( tnf alpha) gene from the marine fish, gilthead seabream (sparus aurata l.), has been isolated by rt-pcr using degenerate primers designed against vertebrate tnf alpha conserved motifs and subsequent rapid amplification of cdna ends (race). the tnf alpha cdna consists of a 142 bp 5' untranslated region (5'utr), a single open reading frame of 762 bp, which could code for a 253 amino acid protein, and a 476-bp 3'utr. the protein sequence deduced from seabream tnf a ... | 2002 | 12073149 |
| sea bream sparus aurata, an asymptomatic contagious fish host for nodavirus. | during an epidemiological survey of viral encephalopathy and retinopathy (ver) in diseased sea bass dicentrarchus labrax, a nodavirus isolate was recovered from net pen-reared sea bream sparus aurata harboured in the same farming premises. after the virus was isolated and identified by immunofluorescence on ssn-1 cells, sequence analysis with a pcr product from the t4 region of the capsid protein gene indicated that the virus shared 100% identity with a pathogenic virus strain isolated from sea ... | 2001 | 11797913 |
| developmental expression of alcohol dehydrogenase (adh3) in zebrafish (danio rerio). | alcohol dehydrogenase (adh) is the primary enzyme responsible for metabolism of ethanol to acetaldehyde. one class of adh has been described in fish, and has been found to be structurally similar to mammalian class iii adh (glutathione-dependent formaldehyde dehydrogenase) but functionally similar to class i adh (primarily responsible for ethanol metabolism). we have cloned a cdna by rt-pcr from zebrafish (danio rerio) liver representing the zebrafish adh3 gene product, with a coding region of 1 ... | 2001 | 11527411 |
| predictive modeling of the shelf life of fish under nonisothermal conditions. | the behavior of the natural microflora of mediterannean gilt-head seabream (sparus aurata) was monitored during aerobic storage at different isothermal conditions from 0 to 15 degrees c. the growth data of pseudomonads, established as the specific spoilage organisms of aerobically stored gilt-head seabream, combined with data from previously published experiments, were used to model the effect of temperature on pseudomonad growth using a belehradek type model. the nominal minimum temperature par ... | 2001 | 11282639 |
| developmental expression, tissue distribution and hormonal regulation of fish (sparus aurata) serum retinol-binding protein. | retinol-binding protein (rbp) is the specific carrier of retinol in vertebrates and forms a 1:1 complex with transthyretin (ttr). a cdna encoding serum rbp was cloned from liver and 7-day larvae of the marine fish sparus aurata. the mature protein is 176 amino acids long and shows sequence identity of 77-78%, 56%, 63% and 62% with rainbow trout, xenopus, chicken and human rbp, respectively. northern blot analysis of hepatic rbp revealed two transcripts: a major one of approximately 1.4-1.5 kb an ... | 2001 | 11399497 |
| cloning of the bone gla protein gene from the teleost fish sparus aurata. evidence for overall conservation in gene organization and bone-specific expression from fish to man. | bone gla protein (bgp, osteocalcin) is a bone-specific vitamin k-dependent protein which has been intensively studied in mammals. although bgp is the most abundant non-collagenous protein of bone, its mode of action at the molecular level remains unclear. from an evolutionary point of view, the appearance of bgp seems to parallel the appearance of hydroxyapatite-containing bone structures since it has never been found in elasmobranchs, whose skeleton is composed of calcified cartilage. according ... | 2001 | 11404005 |
| characterization of a sea bream (sparus aurata) thyroid hormone receptor-beta clone expressed during embryonic and larval development. | a clone encoding thyroid hormone receptor-beta (tr-beta) was isolated from a sea bream (sparus aurata) ovary cdna library. sea bream (sb)tr-beta is closely related to its counterparts from other vertebrates and, like them, preferentially binds t3 rather than t4. however, the putative sbtr-beta protein contains a nine-amino-acid insert that is also present in the corresponding proteins from flounder and salmon but absent in tr-betas from zebrafish and terrestrial vertebrates. semiquantitative rt- ... | 2001 | 11551120 |
| molecular cloning and sequence of sparus aurata skeletal myosin light chains expressed in white muscle: developmental expression and thyroid regulation. | two full-length cdna clones encoding the skeletal myosin light chain 2 (mlc2; 1452bp) and myosin light chain 3 (mlc3; 972bp) were isolated from a cdna library prepared from gilthead sea bream sparus aurata larvae. the mlc2 cdna encoded a predicted protein of 170 residues that was 79% identical to rabbit mlc2 over the entire length and 87% identical within the ca(2+)-binding region. the deduced amino acid sequence of mlc3 was 153 residues in length and was 91% and 69% identical to the zebrafish a ... | 2001 | 11551989 |
| cymothoid parasite ceratothoa parallela inflicts great losses on cultured gilthead sea bream sparus aurata in greece. | for the first time ceratothoa parallela (otto, 1828), a cymothoid isopod, is reported parasitizing cage-cultured gilthead sea bream sparus aurata, in greece. the specimens observed are larvae (pullus secundus). they were found in the branchial and buccal cavity of young gilthead sea bream of 2 g mean body weight, upon introduction in the cages in an intensive cage farm facility. the species was previously known from several species of wild fish, particularly sparidae (chiefly boops boops) in the ... | 2001 | 11558733 |
| glyconjugates in epidermal, branchial and digestive mucous cells and gastric glands of gilthead sea bream, sparus aurata, senegal sole, solea senegalensis and siberian sturgeon, acipenser baeri development. | epidermal, branchial and digestive mucous cells, and the gastric glands of larvae/postlarvae (from hatching until 45 days posthatching) of three fish species (two teleostean and a chondrostean) were investigated using conventional histochemical methods (periodic acid schiff -pas-, diastase-pas; alcian blue ph 0.5, 1 and 2.5) in order to distinguish neutral and acidic (carboxylated and sulphated) glycoconjugates, as well as bromophenol blue reaction for identification of proteins. additionally, t ... | 2001 | 11759813 |
| cymothoid isopod parasites in aquaculture: a review and case study of a turkish sea bass (dicentrarchus labrax) and sea bream (sparus auratus) farm. | this paper reviews the occurrence of cymothoid isopod parasitism in aquaculture, reports the first case of infection by a cymothoid isopod (ceratothoa oestroides) in turkish aquaculture, and analyses its effects on sea bass dicentrarchus labrax. analyses revealed that c. oestroides negatively affects the weights and lengths of sea bass hosts. these effects have been previously underestimated because host age has not been accounted for. the analysis of condition factors as a means of assessing pa ... | 2001 | 11710552 |
| hydrophobicity and adhesion to fish cells and mucus of vibrio strains isolated from infected fish. | the hydrophobicity of 44 vibrio strains isolated from cultured, diseased gilt-head sea bream (sparus aurata) was determined. three different methods were used: (1) microbial adhesion to hydrocarbons (math), either with phosphate buffer or with phosphate urea magnesium sulfate (pum) buffer, (2) aggregation in the presence of salt solutions (sat), and (3) adhesion to nitrocellulose filters (ncf). the results show that experimental conditions exerted a significant influence on hydrophobicity. thus, ... | 2001 | 11770816 |
| gonadal steroidogenesis in response to estradiol-17beta administration in the sea bream (sparus aurata l.). | the sea bream (sparus aurata) is a protandrous hermaphrodite teleost fish in which estrogen administration induces testicular regression without influencing ovarian development. to analyze the changes in steroidogenesis of fish treated with two levels of estrogen (2 and 10 mg. kg(-1)) and untreated control fish, fragments of gonads were incubated with tritiated 17-hydroxyprogesterone and the metabolites identified. the ability to extract radioactivity decreased with incubation time and was lower ... | 2001 | 11703074 |
| molecular components related to egg viability in the gilthead sea bream, sparus aurata. | in pelagic egg spawners, the production of large numbers of sinking eggs, unable to develop into embryos, represents one of the major limiting factors in controlled reproduction. the aim of this study is to elucidate the molecular differences between floating and nonfloating eggs at cytoplasmic and nuclear level. comparison of analyses between floating and nonfloating sea bream sparus aurata eggs evidenced differences in vitelline envelope protein components, such differences being probably rela ... | 2001 | 11170274 |
| cloning, characterization, and tissue distribution of prolactin receptor in the sea bream (sparus aurata). | the prolactin receptor (prlr) was cloned and its tissue distribution characterized in adults of the protandrous hermaphrodite marine teleost, the sea bream (sparus aurata). an homologous cdna probe for sea bream prlr (sbprlr) was obtained by rt-pcr using gill mrna. this probe was used to screen intestine and kidney cdna libraries from which two overlapping clones (1100 and 2425 bp, respectively) were obtained. these clones had 100% sequence identity in the overlapping region (893 bp) and were us ... | 2001 | 11161768 |
| pituitary and interrenal function in gilthead sea bream (sparus aurata l., teleostei) after handling and confinement stress. | dynamics of adrenocorticotropin (acth), alpha melanocyte-stimulating hormone (alpha-msh), n-acetylated-beta-endorphin (n-ac-beta-end), cortisol, and growth hormone (gh) were investigated in gilthead sea bream (sparus aurata) stressed by handling plus confinement. as indices of the secondary stress response, plasma levels of glucose, lactate, and plasma ions were monitored. within 1 h, plasma cortisol and acth levels increased above the control values but gh levels decreased. subsequently, at 24 ... | 2001 | 11254375 |
| cultured fish cells metabolize octadecapentaenoic acid (all-cis delta3,6,9,12,15-18:5) to octadecatetraenoic acid (all-cis delta6,9,12,15-18:4) via its 2-trans intermediate (trans delta2, all-cis delta6,9,12,15-18:5). | octadecapentaenoic acid (all-cis delta3,6,9,12,15-18:5; 18:5n-3) is an unusual fatty acid found in marine dinophytes, haptophytes, and prasinophytes. it is not present at higher trophic levels in the marine food web, but its metabolism by animals ingesting algae is unknown. here we studied the metabolism of 18:5n-3 in cell lines derived from turbot (scophthalmus maximus), gilthead sea bream (sparus aurata), and atlantic salmon (salmo salar). cells were incubated in the presence of approximately ... | 2001 | 11269695 |
| immunocytochemical and ultrastructural characterization of somatolactin cells from the gilthead sea bream (sparus aurata l., teleostei): an ontogenic study (from newly hatched to adults). | for the first time, somatolactin (sl) cells have been ultrastructurally identified and characterized during the ontogeny of gilthead sea bream, sparus aurata, using specimens ranging in age from hatching to 15 months. the sl cells were identified by an immunogold method using anti-cod sl serum. the sl-immunoreactivity was mostly located on the secretory granules of the cells, although some vesicles of variable size and shape with a medium electron-dense content, and some irregular secretory gran ... | 2001 | 11453162 |
| histopathology of cultured sea bream sparus aurata infected with sanguinicolid trematodes. | the present study is the first report of a sanguinicolid infection affecting sea bream sparus aurata cultured in net cages in the ne of spain. the disease was associated with trickling mortalities during the cold season (1999 and 2000). examination of gill wet mounts of the affected population revealed that sanguinicolid infection was present in 82.6 and 100% of the fish sampled in 1999 and 2000, respectively. adult flukes, which were located in the kidney, were tentatively identified as members ... | 2001 | 11253874 |
| thyroid hormones in growth and development of fish. | the thyroid hormones (ths), thyroxine (t(4)) and triiodothyronine (t(3)) are products of the thyroid gland in all vertebrates. their role in early development and metamorphosis is well established in mammals and amphibians, respectively, and recently several studies in fish have highlighted the importance of ths during flatfish metamorphosis. ths are present in high quantities in fish eggs and are presumably of maternal origin. during embryogenesis the concentration of t(4) and t(3) in the eggs ... | 2001 | 11738632 |
| parathyroid hormone-related protein: a calcium regulatory factor in sea bream (sparus aurata l.) larvae. | the effects of an n-terminal peptide (amino acids 1-38) of fugu parathyroid hormone-related protein (pthrp 1-38) on calcium regulation of larval sea bream were investigated in seawater (36 per thousand) and after transfer to dilute seawater (12 per thousand). exposure to pthrp 1-38 evoked a 1.5-fold increase in calcium influx in both full-strength and dilute seawater. calcium influx in dilute seawater-adapted larvae was roughly one-half that observed in full-strength seawater controls. pthrp 1-3 ... | 2001 | 11507001 |
| molecular cloning and sequence of gilthead sea bream (sparus aurata) alpha-skeletal actin: tissue and developmental expression. | in the present study, the sparus aurata alpha-skeletal actin was cloned from a mixed larvae complementary dna library. the clone isolated was 1523 bp long with an open reading frame of 1134 bp coding for a 377-amino acid protein. the deduced amino acid sequence of sea bream alpha-actin is identical to fugu alpha-actin-1. the expression of alpha-actin was initiated at the onset of segmentation. in adult fish, alpha-actin is expressed predominantly in white and red muscle. | 2001 | 11470440 |
| effects of beta-naphthoflavone on the cytochrome p450 system, and phase ii enzymes in gilthead seabream (sparus aurata). | the effect of beta-naphthoflavone (beta-nf) on several catalytic activities of cytochrome p450 (cyp) and phase ii enzymes putatively controlled by [ah]-receptor activation in the liver, heart and kidney of gilthead seabream, was investigated. in the liver, beta-nf treatment [intraperitoneal injection (i.p.) 50 mg/kg] resulted in an increase of cyp content, immunoreactive cyp 1a and methoxyresorufin-o-demethylase (merod), pentoxyresorufin o-depentylase (prod) and ethoxyresorufin-o-deethylase (ero ... | 2001 | 11544149 |
| proteins from fish eggs that protect dna from acid precipitation and inhibit dna synthesis. | we partially characterized proteins that inhibit dna acid precipitation from various fish eggs (sparus aurata, dicentrarchus labrax, mugil cephalus and zeus faber). the active proteins were purified by acetone fractionation. the activity was found to be heat resistant. of bivalent cations tested only co(2+) and cu(2+) exerted a profound promoting effect in the activity from all fish. the protein fraction from sparus aurata inhibited dna synthesis in pcr performed by different dna polymerases. th ... | 2001 | 11489434 |
| cytoarchitectonic study of the brain of a perciform species, the sea bass (dicentrarchus labrax). ii. the diencephalon. | the cytoarchitecture of nuclei in the preoptic area, ventral thalamus, dorsal thalamus, epithalamus, hypothalamus, posterior tuberculum, synencephalon, and pretectum and the accessory optic nuclei was analyzed in a perciform teleost, the sea bass dicentrarchus labrax, by using serial sections stained with cresyl-violet. in general, the cytoarchitecture of the preoptic area, ventral and dorsal thalamus, epithalamus, and synencephalon resembles the histological pattern of other teleosts. however, ... | 2001 | 11223930 |
| 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase gene expression is regulated by diet composition and ration size in liver of gilthead sea bream, sparus aurata. | modulation of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (6pf-2-k/fru-2,6-p(2)ase) gene expression by diet composition and ration size was studied in the liver of gilthead sea bream, sparus aurata. from five different types of diet supplied to fish, those with either high carbohydrate/low protein or high carbohydrate/low lipid content stimulated 6pf-2-k/fru-2,6-p(2)ase expression at the levels of mrna, immunodetectable protein and kinase activity as well as promoting higher fructose-2, ... | 2000 | 10760583 |
| molecular cloning, tissue distribution and sequence analysis of complete glucokinase cdnas from gilthead seabream (sparus aurata), rainbow trout (oncorhynchus mykiss) and common carp (cyprinus carpio). | the enzyme glucokinase (gk) (ec 2.7.1.1) plays an important role in the control of glucose homeostasis. qualitative and/or quantitative variations in gk enzyme have been postulated by previous studies to explain why dietary carbohydrate utilisation is lower in gilthead seabream (sparus aurata) and rainbow trout (oncorhynchus mykiss) than in common carp (cyprinus carpio). in this study, we report the isolation and characterisation of a full-length cdna coding for gk in these teleosts. amino acid ... | 2000 | 10699491 |
| proliferation zones in the adult brain of a sequential hermaphrodite teleost species (sparus aurata). | teleost sex change is an important model to understand general principles of sexual differentiation and plasticity in the adult brain. the present study is the first to examine the proliferation zones in the adult brain of males, females and sex-changing individuals of a protandrous teleost species (sparus aurata), by means of 5-bromo-2-deoxyuridine immunocytochemistry. postnatal neurogenesis in the marine teleost brain was found in ventricular and subventricular areas of the brain that in most ... | 2000 | 11326136 |
| identification of mammosomatotropes, growth hormone cells and prolactin cells in the pituitary gland of the gilthead sea bream (sparus aurata l., teleostei) using light immunocytochemical methods: an ontogenetic study. | growth hormone (gh) and prolactin (prl) immunoreactivities in the adenohypophysis of sparus aurata specimens from newly hatched until 48-months-old were detected using the peroxidase-antiperoxidase method. gh cells and prl cells, and cells that were immunoreactive to both gh and prl antisera, called mammosomatotropes (ms cells), were found. this is the first report on the identification of ms cells in fish, which were found in newly hatched and older larvae and juvenile specimens. gh and prl cel ... | 2000 | 11089933 |
| pituitary proopiomelanocortin-derived peptides and hypothalamus-pituitary-interrenal axis activity in gilthead sea bream (sparus aurata) during prolonged crowding stress: differential regulation of adrenocorticotropin hormone and alpha-melanocyte-stimulating hormone release by corticotropin-releasing hormone and thyrotropin-releasing hormone. | plasma levels of cortisol, growth hormone (gh), adrenocorticotropin hormone (acth), alpha-melanocyte-stimulating hormone (alpha-msh), n-acetyl-beta-endorphin, in vitro acth-stimulated cortisol secretion, and in vitro corticotropin-releasing hormone (crh)- and thyrotropin-releasing hormone (trh)-stimulated acth and alpha-msh secretion were investigated in gilthead sea bream exposed to high stocking density (30 kg m(-3)) for 23 days. within 3 days after the onset of crowding, plasma levels of cort ... | 2000 | 10936035 |
| alpha-msh acetylation in the pituitary gland of the sea bream (sparus aurata l.) in response to different backgrounds, confinement and air exposure. | msh is a pituitary hormone derived by post-translational processing from pomc and involved in stress and background adaptation. n-terminal acetylation of msh to monoacetyl alpha-msh or diacetyl alpha-msh increases the bioactivity of the peptide. the aim of this study was to characterize alpha-msh acetylation in the sea bream (sparus aurata l.) pituitary gland in response to the stressors air exposure and confinement, as well as in fish adapted for 15 days to a white, gray or black background. pi ... | 2000 | 10927632 |
| cloning and expression of an elongation factor-1alpha in sea bream (sparus aurata) larvae and adult tissue. | a clone encoding the polypeptide elongation factor ef-1alpha was isolated from a complementary dna library prepared from sea bream (spartus aurata) larvae 1 to 10 days after hatching. the deduced amino acid sequence is between 82% and 95% similar to ef-1alpha in other animal species. ef-1alpha messenger rna is present at low abundance in sea bream embryos prior to gastrulation, but at around 15 hours postfertilization, there is a 10-fold increase in transcript levels. this increase presumably re ... | 2000 | 10811957 |
| liver insulin-like growth factor-i mrna is not affected by diet composition or ration size but shows diurnal variations in regularly-fed gilthead sea bream (sparus aurata). | nutritional regulation of insulin-like growth factor-i (igf-i) mrna was assessed in liver of gilthead sea bream (sparus aurata). as in mammals, starvation lowered the igf-i mrna content, which was recovered by refeeding. however, in contrast to previous observations in rats, neither diet composition nor ration size significantly affected hepatic igf-i mrna. although fish growth depended on the quantity of diet supplied, no relationship was found between growth and liver igf-i mrna levels, a fact ... | 2000 | 10736326 |
| differential splicing of three gonadotropin-releasing hormone transcripts in the ovary of seabream (sparus aurata). | previous studies demonstrated the presence of high-affinity gnrh binding sites and compounds with gnrh-like activity in the ovary of seabream, sparus aurata, providing evidence for the role of gnrh as a paracrine/autocrine regulator of ovarian function in this species. in the present study, the expression of three forms of gnrh (salmon, chicken-ii, and seabream) genes in this marine teleost species was demonstrated for the first time. moreover, there is evidence for differential splicing and int ... | 2000 | 10775184 |
| new methods to use fish cytochrome p4501a to assess marine organic pollutants. | a new methodology has been developed to assess cytochrome p4501a expression in two south atlantic spanish fish, guilthead seabream (sparus aurata) and grey mullet (liza aurata), used as pollution bioindicators. degenerate oligos were used to amplify by reverse transcription and pcr (rt-pcr) specific cyp1a cdna sequences, used subsequently to design specific primers to get the full cdna by rapid amplification of cdna ends. a new assay has been developed to quantitate cyp1a expression by rt-pcr in ... | 2000 | 10803549 |
| evolution of the thyroid hormone-binding protein, transthyretin. | transthyretin (ttr) belongs to a group of proteins, which includes thyroxine-binding globulin and albumin, that bind to and transport thyroid hormones in the blood. ttr is also indirectly implicated in the carriage of vitamin a through the mediation of retinol-binding protein (rbp). it was first identified in 1942 in human serum and cerebrospinal fluid and was formerly called prealbumin for its ability to migrate faster than serum albumin on electrophoresis of whole plasma. it is a single polype ... | 2000 | 11017772 |
| cloning of the cdna for sea bream (sparus aurata) parathyroid hormone-related protein. | this paper reports cloning of the cdna for sea bream (sparus aurata) parathyroid hormone-related protein (pthrp). the gene codes for a 125-amino acid mature protein with a 35-residue prepeptide. the total gene sequence is 1.8 kb with approximately 75% noncoding. the n-terminus of the protein resembles mammalian and chicken pthrp peptides with 12 of the first 21 amino acids identical and for which there is homology with mammalian parathyroid hormone. toward the c-terminus, the nuclear transporter ... | 2000 | 10843788 |
| glucokinase gene expression is nutritionally regulated in liver of gilthead sea bream (sparus aurata). | glucose intolerance in carnivorous fish has been attributed to the lack of hepatic glucokinase (gk) activity. transcription/translation assay and transient transfection of cos-7 cells with a cdna encoding sparus aurata liver gk showed the functionality of the enzyme in vitro. the endogenous fish hepatic gk had lower affinity for glucose than the rat enzyme. the gk activity values in fed fish were similar to those reported for starved and diabetic rats. in this study, we also addressed the nutrit ... | 2000 | 10978515 |
| effects of injecting chitin particles on the innate immune response of gilthead seabream (sparus aurata l.). | to determine the effects of chitin (poly [1-->4]-beta-n-acetyl-d--glucosamine) particles on the innate immune response of gilthead seabream (sparus aurata l.), specimens were injected intravenously or intraperitoneally with the substance. natural haemolytic complement activity, head-kidney leucocyte respiratory burst activity and phagocytic and cytotoxic activities were analysed in vitro 3, 5 or 10 days post-injection. all the immune parameters assayed remained unaffected when chitin was intrave ... | 2000 | 11016588 |
| application of a systematic experimental procedure to develop a microbial model for rapid fish shelf life predictions. | a systematic experimental procedure for fish shelf-life modelling was used to develop a model for predicting the quality of fish in the chill chain. for this, the growth of the naturally occurring bacteria pseudomonads, shewanella putrefaciens, enterobacteriaceae, lactic acid bacteria and yeasts, on gilt-head seabream (sparus aurata), was studied at temperatures from 0 to 15 degrees c. the results from the microbiological, organoleptical and chemical analysis conducted on naturally contaminated ... | 2000 | 11016607 |
| epitheliocystis agents in sea bream sparus aurata: morphological evidence for two distinct chlamydia-like developmental cycles. | the morphology of membrane-bound intracellular inclusions, or 'cysts', of epitheliocystis from sea bream sparus aurata is described. inclusions under the light microscope appear either granular or amorphous. granular inclusions do not elicit a proliferative host reaction and contain the 3 distinctive developmental stages of chlamydial organisms: the highly pleomorphic reproductive form or reticulate body, the condensing form or intermediate body and the infective non-dividing rather uniform elem ... | 1999 | 10439904 |
| molecular cloning and expression of ovarian cathepsin d in seabream, sparus aurata. | in marine fish producing pelagic eggs, the acquirement of buoyancy by the eggs through the hydration process is a key event of reproduction; moreover, the yolk proteolysis, which leads to buoyancy, seems to affect the fertility and survival of the spawned eggs. recently we demonstrated that cathepsin d is the aspartic protease responsible for this intraoocytic processing of vitellogenin into yolk proteins. in the present study, we isolated, cloned, and sequenced the cdna encoding cathepsin d and ... | 1999 | 10456857 |
| cloning of putative piscine (sparus aurata) transthyretin: developmental expression and tissue distribution. | cdna encoding putative transthyretin (prealbumin, ttr) was cloned from liver of the marine fish sparus aurata. the cdna contains an open reading frame of 453 nt, encoding for a ttr precursor of 151 amino acids. the deduced amino acid sequence of s. aurata ttr shows identity of 54, 57.3 and 54.1% with lizard, chicken and rat ttr, respectively. northern blot analysis revealed a ttr transcript of about 700 nt, highly expressed in liver, but also in skin. low expression was detected in 12 other tiss ... | 1999 | 10619398 |
| identification of transthyretin in fish (sparus aurata): cdna cloning and characterisation. | transthyretin (ttr) has been proposed to have first evolved in reptiles and is one of the three plasma proteins important in the transport of thyroid hormones in higher vertebrates. a full-length cdna encoding ttr was isolated from a sea bream (sparus aurata) liver cdna library using a homologous ttr cdna probe generated by rt-pcr. comparison of the deduced amino acid sequence of sea bream ttr with other published sequences, revealed an overall identity of 47-54%, although the amino acids in the ... | 1999 | 10218999 |
| molecular cloning of a cdna encoding 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase from liver of sparus aurata: nutritional regulation of enzyme expression. | a cdna clone encoding full-length 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (6pf-2-k/fru-2, 6-p2ase) was isolated and sequenced from a sparus aurata liver cdna library. the 2527 bp nucleotide sequence of the cdna contains a 73 bp 5'-untranslated region (5'-utr), an open reading frame that encodes a 469 amino acid protein and 1041 bp at the 3'-utr. the deduced amino acid sequence is the first inferred 6pf-2-k/fru-2, 6-p2ase in fish. the kinase and bisphosphatase domains, where the resi ... | 1999 | 10023046 |
| preparation of recombinant gilthead seabream (sparus aurata) growth hormone and its use for stimulation of larvae growth by oral administration. | gilthead seabream (sparus aurata) growth hormone (gsgh) cdna coding for the mature protein was cloned in a pgem-t vector and then transferred into prokaryotic expression vector pet-8 and expressed in e. coli bl21 (de3) cells upon induction with iptg. the expressed protein, contained within the inclusion-body pellet, was solubilized in 4.5 m urea, refolded at ph 11.3 in the presence of catalytic amounts of cysteine, and purified to over 98% purity, as evidenced by sds-page. gel-filtration on a su ... | 1999 | 9882554 |
| seasonal changes of zinc, copper, and iron in gilthead sea bream (sparus aurata) fed fortified diets. | four groups of gilthead sea bream (sparus aurata) were fed diets with additional metal contents: a basal diet (diet a) contained zn at 60.9 +/- 1.9 mg/kg diet, cu at 3.9 +/- 0.9 mg/kg diet, and fe at 138.3 +/- 6.8 mg/kg diet; the other diets were supplemented with copper (20 mg/kg, diet b), iron (100 mg/kg, diet c), or zinc (300 mg/kg, diet d). two consecutive year-classes (0+ and 1+ age fish) from the same parent stock were examined. several fish tissues were analyzed for metal contents in five ... | 1999 | 10433345 |
| cloning and sequencing of the gilthead sea bream estrogen receptor cdna. | we report here the complete nucleotide sequence of a cdna clone containing the full-coding sequence of the sparus aurata estrogen receptor (er) isolated from an expression library prepared from gilthead sea bream liver poly a+ rna. the library was screened using a single strand rainbow trout er cdna probe, corresponding to the c-d domain. the cdna sequence containing an insert of 2369 nucleotides was found to encode a protein of 579 amino acids. the 5'- and 3'-untranslated regions of the message ... | 1999 | 10376207 |
| red muscle development of gilthead sea bream sparus aurata (l.): structural and ultrastructural morphometry. | the transverse red and white muscle area, the superficial red muscle fibres area and their percentages of mitochondria (%mit), myofibrils (%myof) and sarcoplasm (%sarc) were determined in the mediterranean teleost gilthead sea bream, sparus aurata (l.). fish aged from hatching to 78 days were studied. the proportional growth of the red and white muscles was higher for the red muscle in the first half of the larval stage (1-35 days). then the opposite relationship was observed. the hypertrophic g ... | 1999 | 10208029 |
| glomerular disease associated with polysporoplasma sparis (myxozoa) infections in cultured gilthead sea bream, sparus aurata l. (pisces: teleostei). | polysporoplasma sparis infection was studied in gilthead sea bream from different mariculture systems of the spanish coasts. culture conditions influenced the infection dynamics, as the parasite appeared only in semi-intensive cultures and was not found in intensive closed systems nor in open ones. no clear seasonal pattern was observed. no fish weighing less than 51 g was found parasitized in any group. a statistically significant dependence between infection prevalence and host weight was obse ... | 1999 | 10205800 |
| cloning, expression, and tissue localisation of prolactin in adult sea bream (sparus aurata). | a major action of prolactin (prl) in teleost fish is the maintenance of hydromineral balance in euryhaline species in fresh water. the function of prl in marine teleosts is less certain and unlike euryhaline teleosts, such as tilapia and salmon, there is relatively little information about protein or gene structure. associated with studies to determine potential functions of prl, pituitary prolactin cdna has been cloned and sequenced from sea bream (sparus aurata), a marine teleost. the sequence ... | 1999 | 10094859 |
| effect of diet composition and ration size on key enzyme activities of glycolysis-gluconeogenesis, the pentose phosphate pathway and amino acid metabolism in liver of gilthead sea bream (sparus aurata). | the effects of diet composition and ration size on the activities of key enzymes involved in intermediary metabolism were studied in the liver of gilthead sea bream (sparus aurata). high-carbohydrate, low-protein diets stimulated 6-phosphofructo 1-kinase (ec 2.7.1.11), pyruvate kinase (ec 2.7.1.40), glucose-6-phosphate dehydrogenase (ec 1.1.1.49) and 6-phosphogluconate dehydrogenase (ec 1.1.1.44) enzyme activities, while they decreased alanine aminotransferase (ec 2.6.1.2) activity. a high degre ... | 1999 | 10655969 |
| unilateral eye abnormalities in reared mediterranean gilthead sea bream. | eye abnormalities in reared gilthead sea bream (sparus aurata) were investigated clinically and by histological techniques. a significant number of fish had a cataract in one eye and ocular inflammation including gross exophthalmos. in a small number of fish gas bubbles were observed in the anterior chamber of the eye. histological examination provided no evidence of an infectious process and a possible behavioural aetiology is discussed. | 1999 | 10596872 |
| the effect of estrogen on the gonads and on in vitro conversion of androstenedione to testosterone, 11-ketotestosterone, and estradiol-17beta in sparus aurata (teleostei, sparidae). | the effects of estrogen on gonad morphology and steroidogenesis of sea bream, sparus aurata, a protandrous hermaphrodite teleost, were investigated. fish were treated in winter/spring for different periods with 17alpha-ethynylestradiol (ethe(2); experiment 1) and in summer with two doses of estradiol-17beta (e(2); experiment 2). estrogen was more effective in summer. its main effect on the gonad was inhibition of testicular growth and of male germ cell development beyond the spermatogonia stage, ... | 1999 | 10525362 |
| the stress response of the gilthead sea bream (sparus aurata l.) to air exposure and confinement. | we investigated short-term effects (up to 24 h) of air exposure and confinement, and long-term effects (up to 11 days) of confinement, to elucidate signalling pathways in the stress response of gilthead sea bream sparus aurata l. plasma glucose and lactate were taken as indicators of sympathetic activation, and alpha-melanocyte stimulating hormone (alpha-msh), adrenocorticotrophic hormone (acth) and cortisol as indicators of activation of the brain-pituitary-interrenal (bpi) axis. air exposure f ... | 1999 | 10495417 |
| biogenic amines and sensory changes associated with the microbial flora of mediterranean gilt-head sea bream (sparus aurata) stored aerobically at 0, 8, and 15 degrees c. | changes in the concentrations of tyramine, agmatine, putrescine, cadaverine, spermidine, tryptamine, spermine, histamine, and trimethylamine were studied in parallel with the development of the microbial population during the storage of mediterranean gilt-head sea bream (sparus aurata) at three temperatures (0, 8, 15 degrees c). changes in sensory scores were also recorded. pseudomonads and h2s-producing bacteria were the dominant microorganisms. enterobacteriaceae and lactic acid bacteria were ... | 1999 | 10419215 |
| fatty acid metabolism in marine fish: low activity of fatty acyl delta5 desaturation in gilthead sea bream (sparus aurata) cells. | marine fish have an absolute dietary requirement for c20 and c22 highly unsaturated fatty acids. previous studies using cultured cell lines indicated that underlying this requirement in marine fish was either a deficiency in fatty acyl delta5 desaturase or c18-20 elongase activity. recent research in turbot cells found low c18-20 elongase but high delta5 desaturase activity. in the present study, the fatty acid desaturase/elongase pathway was investigated in a cell line (saf-1) from another carn ... | 1999 | 10380114 |
| dietary chromic oxide does not affect the utilization of organic compounds but can alter the utilization of mineral salts in gilthead sea bream sparus aurata. | this study was conducted to determine whether the level of chromic oxide supplemented to diets containing gelatinized starch as the carbohydrate source affects digestibility, body composition, growth performances, and liver enzyme activities in gilthead sea bream, sparus aurata. gilthead sea bream fingerlings were fed diets containing gelatinized corn starch as the carbohydrate source and several levels of chromic oxide (0, 5, 10 and 20 g/kg) for 6 wk. no effect of dietary chromium level was det ... | 1999 | 10222399 |
| melatonin release from the pineals of two sparids. sparus aurata and acanthopagrus bifasciatus. | 1999 | 10810502 | |
| negative covariance suggests mutation bias in a two-locus microsatellite system in the fish sparus aurata. | constraints on microsatellite length appear to vary in a species-specific manner. we know very little about the nature of these constraints and why they should vary among species. while surveying microsatellite variation in the mediterranean gilthead sea bream, sparus aurata, we discovered an unusual pattern of covariation between two closely linked microsatellite loci. one- and two-locus haplotypes were scored from pcr amplification products of each locus separately and both loci together. in a ... | 1998 | 9832532 |
| hepatic steatosis in artificially fed marine teleosts. | in a comparison of livers in fish (sparus auratus and dicentrarchus labrax) feeding on natural sources of food with livers of artificially fed animals, a much higher c18:1/c22:6 ratio was observed in the latter. staining livers with oil red o showed extensive steatosis in artificially fed fish, but not in those naturally fed. juvenile artificially fed fish showed a more extensive steatosis and a higher mortality rate. in steatotic fish fed a natural diet for 2 months, the liver exhibited extensi ... | 1998 | 21361972 |
| histochemical study of lymphocystis disease in skin of gilthead seabream, sparus aurata l. | a battery of horseradish peroxidase-conjugated lectins (con a, wga and dba), as well as conventional histochemical techniques (pas, saponification, alcian blue ph 0.1, 1, 2.5, chlorhydric hydrolisis, sialidase, bromophenol blue, tioglycollate reduction and ferric-ferricyanide-feiii) were used to study the content and distribution of carbohydrates, proteins and glycoconjugate sugar residues on the skin and on the lymphocystis-infected cells of gilthead seabream, sparus aurata. variable amounts of ... | 1998 | 9476632 |
| muscle development in gilthead sea bream (sparus aurata, l.) and sea bass (dicentrarchus labrax, l.): further histochemical and ultrastructural aspects. | the histochemical profiles--matpase and nadh-tr reactions--of the red and white muscle fibres of gilthead sea bream and sea bass were determined from the first week after hatching. modifications of the matpase technique by combinations of ph/time/molarity were carried out in order to compare the sensitivity of the myosin atpase of each muscle fibre type of the lateral muscle. results showed that the staining of muscle fibres was independent of small modifications in the technique. the intermedia ... | 1998 | 9741144 |
| insulin during the early postembryonic development of the gilt-head sea bream, sparus aurata: ultrastructural, immunohistochemical, and biochemical studies. | insulin was sought during the early postembryonic development of gilt-head sea bream, sparus aurata, using ultrastructural, immunohistochemical and biochemical techniques. the endocrine pancreas appeared at hatching (day 0) as a single cluster of morphologically similar cells. secretory granules formed from day 1 onwards but the cells could only be identified as insulin-producing b cells at the end of the endotrophic period (day 3-day 4). insulin-immunoreactive cells were detected in the pancrea ... | 1998 | 9570935 |