Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| experimental trichinosis: parasitological, electrophysiological and biochemical studies. | trichinosis is a parasitic infection affecting the gut and the muscles causing mild gastrointestinal symptoms followed by periorbital oedema, muscle pains, fever and eosinophilia. the infection evokes functional disturbances in physiological effector systems. furthermore, several biochemical changes are associated with the infection. therefore, this work was carried out to study the electrophysiological changes in intestine, striated and cardiac muscles by electromyography (emg) and to assess th ... | 2006 | 16927861 |
| [study on genetic approach in the detection of cryptosporidium parvum and giardia lamblia in acquired immunodeficiency syndrome patients]. | to establish genetic method in detecting cryptosporidium parvum and giardia lamblia which often coinfected with aids patients. | 2006 | 17343184 |
| [serum il-12 level in mice infected with trichinella spiralis]. | kunming mice were infected by feeding 150+/-5 larvae of trichinella spiralis, established was also a normal control group. blood was collected from the ophthalmic venous plexus respectively on 7 d, 21 d, 35 d and 49 d after infection and il-12 in the serum was detected by elisa. the level of il-12 in serum decreased in groups of 7 d, 21 d, and 35 d, with a significant difference to the control (p<0.01). however, there was no significant difference between 49d group and control (p>0.05), suggesti ... | 2006 | 17366986 |
| trichinellosis in human and animals--new challenge. proceedings of a conference, warsaw, poland,2006. | 2006 | 17511047 | |
| [biology, species biodiversity and distribution of trichinella nematodes]. | from the time of the discovery of trichinella larvae in 1835 until the middle of the next century it was commonly assumed that all trichinellosis was caused by a single species trichinella spiralis. this species is an intracellular parasite in both a larva and an adult stage. the l1 larvae live in a modified skeletal muscles. the adult worms occupy a membrane-bound portion of columnar epitelium, living as intramulticellular parasite. more than century later t. spiralis have been reported from mo ... | 2006 | 17432238 |
| [methods and tools for parasite differentiation within the genus trichinella]. | this review summarizes the major biological, biochemical and molecular methods which have been developed during last 20 years to distinguish parasites of the genus trichinella. from the time of the discovery of trichinella in 1835 until the 1970, it was assumed that trichinellosis was caused by a single species of parasite, trichinella spiralis. many biological parameters have been compared to differentiate the parasite, such as host specificity, geographical distribution, reproductive abilities ... | 2006 | 17432239 |
| [the influence of the procedure of excretory-secretory l1 trichinella spiralis antigen preparation on the efficiency of an elisa test in pigs]. | trichinellosis is a parasitic zoonosis transmitted to humans by consumption of raw or undercooked meat from animals infected by worms of the trichinella genus. every year seropositive cases are found among the human population and thus trichinellosis still remains an epidemiologically important disease in poland. the usefulness of elisa for anti-t. spiralis igg detection in pigs is still limited by the nature of antigen. the objective in the present study was to compare the usefulness of excreto ... | 2006 | 17432246 |
| the estimation of different elisa procedures for serodiagnosis of human trichinellosis. | the most important confirmative diagnostic test for trichinellosis is the presence of the muscle larvae in a tissue biopsy but this direct method has a low sensitivity of light and moderate infections. the aim of presented study was to compare the usefulness of the results obtained by three elisa procedures for trichinella spp. diagnosis in human outbreaks. | 2006 | 17432247 |
| [influence of free radicals on trichinella spiralis infection in mice]. | the aim of the study was to examine the influence of free radicals: nitric oxide (no), hydrogen peroxide (h202) and superoxide anion (o2-) on trichinella spiralis infection in mice. the studies were performed on two strains of mice: c57bl/6 and balb/c, which differ in immunological response to t. spiralis infection. also the influence of ag--inhibitor of inducible nitric oxide synthase (inos) administered in the first days after t. spiralis infection (1-5 dpi) on the cytotoxic immune response an ... | 2006 | 17432248 |
| biology and genome of trichinella spiralis. | clade i nematode species in the genus trichinella can cause infections in humans that lead to mortality and serious morbidity. there are currently eight recognized species or genotypes that comprise this genus. the species display diverse biological characteristics, the evolutionary significance of which recently has been extensively clarified. some of that diversity translates into variable importance as zoonotic pathogens, with t. spiralis having the highest significance. trichinellosis has re ... | 2006 | 18050431 |
| a recombinant enolase from anisakis simplex is differentially recognized in natural human and mouse experimental infections. | a 1,963-bp cdna was isolated from an anisakis simplex cdna library by immunoscreening with a hyperimmune rabbit serum raised against a crude extract of a. simplex l3 larvae. the open reading frame encodes a putative protein of 436 amino acid residues, which exhibits high similarity (70-80%) to enolase molecules from various other organisms, including helminth parasites. after subcloning and expression of the a. simplex cdna in pgex-4t-3, the resulting glutathione s-transferase fusion protein, pu ... | 2006 | 16049725 |
| the involvement of the macrophage mannose receptor in the innate immune response to infection with parasite trichinella spiralis. | the macrophage mannose receptor (mr) is a pattern recognition receptor of the innate immune system that binds to microbial structures bearing mannose, fucose and n-acetylglucosamine on their surface. the mr can mediate endocytosis and phagocytosis, as well as activation of macrophages and antigen presentation. since trichinella spiralis antigens are rich in oligomannose residues, we investigated whether a mannose-recognizing receptor, such as the mr, participated in the host-parasite interaction ... | 2006 | 16135385 |
| a comparison of regional and systemic humoral immune responses to a nematode infection. | the kinetics of humoral immune response against trichinella spiralis (ts) was characterized with immunofluorescence assay. the mesenteric lymph nodes (mln) and the spleen of infected rats were examined for concurrent expression of multiple antibody (ab) isotypes from day 1 to day 15 after infection. the tissues were processed and stained with either a pan-b cell marker (ox33) conjugated with rhodamine (xritc) or combinations of dual monoclonal ab probes plus a secondary ab conjugated with xritc ... | 2006 | 16146759 |
| vaccination of mice with dna vaccine induces the immune response and partial protection against t. spiralis infection. | trichinellosis is a serious parasitic zoonosis which is widely distributed in the world. pork still is the predominant source of outbreaks of human trichinellosis in many countries. vaccines are urgently needed to prevent swine from trichinella infection. the gene (tspe1) encoding a 31 kda antigen of t. spiralis was cloned to a eukaryotic expression plasmid pcdna3 as dna vaccine. the ability of the dna vaccine to express antigen in mammalian cho cells was previously confirmed by indirect fluores ... | 2006 | 16202484 |
| trichinella spiralis: macrophage activity and antibody response in chronic murine infection. | the role of macrophages, their products, and the specific antibody response were examined during chronic trichinella spiralis infection in balb/c mice. adult t. spiralis in intestines were detected from 5 to 20 dpi. muscle larvae numbers peaked at 45 dpi and thereafter a reduction was noted. the highest numbers of macrophages in the peritoneal cavity of infected mice were obtained up to 30 dpi. the production of no by macrophages in infected mice was suppressed at 5 dpi, and then no release incr ... | 2006 | 16274689 |
| sample size calculations for disease freedom and prevalence estimation surveys. | we developed a bayesian approach to sample size calculations for studies designed to estimate disease prevalence that uses a hierarchical model for estimating the proportion of infected clusters (cluster-level prevalence) within a country or region. the clusters may, for instance, be villages within a region, cities within a state, or herds within a country. our model allows for clusters with zero prevalence and for variability in prevalences among infected clusters. moreover, uncertainty about ... | 2006 | 16287201 |
| characterisation of a secreted n-acetyl-beta-hexosaminidase from trichinella spiralis. | a thorough investigation was conducted for glycoside hydrolase activities in the secreted proteins of trichinella spiralis. the data demonstrated that the only secreted glycosidase with significant activity was an exo-beta-hexosaminidase with catalysis of the substrates n-acetyl-beta-d-glucosamine, n-acetyl-beta-d-galactosamine and n-acetyl-beta-d-glucosamine-6-sulphate proceeding with an efficiency similar to the human isozyme beta-hexosaminidase a (hex a). the hydrolysis of n-acetyl-beta-d-glu ... | 2006 | 16242793 |
| immune control of food intake: enteroendocrine cells are regulated by cd4+ t lymphocytes during small intestinal inflammation. | gastrointestinal inflammation reduces food intake but the biological mechanisms explaining suppressed feeding during inflammation are unknown. we have used a model of upper gut infection (trichinella spiralis in the mouse) to study the effect of inflammation on food intake, and explored the role of a key enteroendocrine cell (eec) in the regulation of feeding by the immune response. | 2006 | 16299028 |
| fusion and differentiation of murine c2c12 skeletal muscle cells that express trichinella spiralis p43 protein. | the ability of a 43 kda stichocyte protein from trichinella spiralis (tsp43) to interfere with mammalian skeletal muscle gene expression was investigated. a myc-tagged tsp43 construct was expressed as a recombinant protein in c2c12 myoblasts. transfection with low amounts of expression plasmid was required for successful expression of the protein. this construct had apparent toxic effects on transfected myoblasts and ectopic green fluorescent protein expression was suppressed in myoblasts co-tra ... | 2006 | 16330028 |
| potential use of trichinella spiralis antigen for serodiagnosis of human capillariasis philippinensis by immunoblot analysis. | intestinal capillariasis is an emerging helminthic zoonosis caused by capillaria philippinensis and is frequently fatal if not diagnosed correctly. the present study demonstrates cross-reactivity between trichinella spiralis larval antigens and c. philippinensis-infected human sera by immunoblotting. sera from 16 proven intestinal capillariasis patients and 16 proven trichinosis patients were tested. the antigenic patterns recognized by intestinal capillariasis sera varied with the molecular mas ... | 2006 | 16333664 |
| ox40 interactions in gastrointestinal nematode infection. | the immune expulsion of gastrointestinal nematode parasites is usually associated with t helper type 2 (th2) responses, but the effector mechanisms directly responsible for parasite loss have not been elucidated. the intestinal inflammatory response accompanying infection with gastrointestinal helminths is thought to be a contributory factor leading to the expulsion of the parasite. however, we have shown that the intestinal inflammation, which is controlled by interleukin (il)-4, is not require ... | 2006 | 16423046 |
| vaccination of mice against intestinal trichinella spiralis infections by oral administration of antigens microencapsulated in methacrilic acid copolymers. | balb/c and nih mice have been successfully vaccinated against the intestinal nematode trichinella spiralis by oral administration of crude larval extracts (cle) and excretory-secretory (es) products derived from first stage t. spiralis larvae (l1) encapsulated in microcapsules made of copolymers of the metacrylic acid (eudragit l100). oral vaccination stimulated the secretion of ifn-gamma and inhibited the secretion of il-4 in spleen and mesenteric lymph nodes (mln) of balb/c mice. in vaccinated ... | 2006 | 16446017 |
| induction of enhanced immunity to intestinal nematodes using il-9-producing dendritic cells. | dendritic cells can be considered natural adjuvants and are able to act as cellular vaccines to protect against disease. adoptive transfer of ag-pulsed bone marrow-derived dendritic cells (bmdcs) enhanced expulsion of the intestinal nematode, trichinella spiralis, from the small intestine. il 9 is a critical cytokine in protective immunity to intestinal nematode infection and is believed to enhance th2 immune responses. deriving dendritic cells from an il-9 transgenic (il-9t) mouse has enabled a ... | 2006 | 16456011 |
| chromosomal mapping of host resistance loci to trichinella spiralis nematode infection in rats. | the differences in host response among strains of rats to intestinal nematode parasite trichinella spiralis infection could provide a powerful benefit for further elucidation of molecular interactions between the host and the parasite. using several strains of rats, we previously observed that da strain is a strong responder and f344 strain is a weak responder with respect to expulsion of the adult worm. to identify the host resistance loci, quantitative trait loci (qtls) analysis in f2 populati ... | 2006 | 16465509 |
| long-lasting changes in small intestinal transport following the recovery from trichinella spiralis infection. | changes in intestinal motility and visceral sensitivity are found after resolution of acute enteric inflammation. the study investigates whether a transient nematode-induced intestinal inflammation may result in long-lasting remodelling of epithelial transport. ferrets infected with trichinella spiralis or sham-infected animals were euthanized on day 10, 30 or 60 postinfection (pi) and the jejunum was isolated. the net transport of electrolytes was measured electrophysiologically as transmucosal ... | 2006 | 16487415 |
| cell-mediated response at the muscle phase of trichinella pseudospiralis and trichinella spiralis infections. | the cell-mediated response in balb/c mice infected either by trichinella pseudospiralis or trichinella spiralis was compared at days 30-50 post-infection (muscle phase). the former species is non-encapsulated, whereas the latter is encapsulated in host muscles. the pattern of response against the two species was similar. both species elicited t(h)0 or t(h)1/t(h)2 response, with the last one being dominant. productions of interferon gamma (ifn-gamma), interleukin (il)-4 and il-5 were observed aft ... | 2006 | 16489472 |
| amelioration of influenza-induced pathology in mice by coinfection with trichinella spiralis. | illness due to respiratory virus infection is often induced by excessive infiltration of cells into pulmonary tissues, leading to airway occlusion. we show here that infection with trichinella spiralis results in lower levels of tumor necrosis factor in bronchoalveolar lavage fluid and inhibits cellular recruitment into the airways of mice coinfected with influenza a virus. infiltration of neutrophils and cd4+ and cd8+ lymphocytes was reduced, resulting in animals gaining weight more rapidly fol ... | 2006 | 16495568 |
| synthesis of syndecan-1 by skeletal muscle cells is an early response to infection with trichinella spiralis but is not essential for nurse cell development. | trichinella spiralis creates a unique intracellular habitat in striated muscle. we report that a proteoglycan, syndecan-1, is induced early in infection yet is not essential for habitat development and exerts a modest influence on the immune response. this report is the first to address the requirement for a specific muscle protein in trichinellosis by using mice deficient in the relevant gene. | 2006 | 16495570 |
| trichinella spiralis and trichinella pseudospiralis mixed infection in a wild boar (sus scrofa) of germany. | a wild boar (sus scrofa) from the island usedom in mecklenburg-western pomerania (north-east germany) was detected as trichinella-positive during routine meat inspection. encapsulated and non-encapsulated larvae were detected in the muscle tissue by trichinoscopy. in the diaphragm, 922 larvae per g were detected by artificial digestion. muscle larvae displayed two different sizes of about 700 and 1100 microm. by a multiplex pcr analysis, larvae with a large size were identified as trichinella sp ... | 2006 | 16513281 |
| inhibition of nitric oxide production by aminoguanidine influences the number of trichinella spiralis parasites in infected "low responders" (c57bl/6) and "high responders" (balb/c) mice. | the influence of nitric oxide (no) on the development of adults and larvae trichinella spiralis was examined in two strains of mice: c57bl/6 and balb/c. the influence of aminoguanidine (ag)-inhibitor of inducible nitric oxide synthase (inos) administered in the first days after t. spiralis infection (1-5 dpi) on the number of adult parasites, as well as the influence of ag administered at the beginning of muscle phase of the t. spiralis infection (16-29 dpi) on the number of muscle larvae, was s ... | 2006 | 16541259 |
| [epidemiological trends and control measures of trichinellosis in china]. | 2005 | 16562466 | |
| intercellular adhesion molecule-2 (icam-2) in experimental trichinosis. | the estimation of the serum adhesion molecules (icam-2) levels in experimental trichinosis and evaluation of its impact on the course of infection as well as its possible role in the diagnosis of early trichinosis were studied. serum levels of icam-2 correlated significantly with the inflammatory and course sequences of trichinosis in mice and had a similar relation with blood eosinophilia. so, estimation of icam-2 serum levels may prove useful in diagnosis of trichinosis recent infections, and ... | 2005 | 16333907 |
| enterochromaffin cell hyperplasia and decreased serotonin transporter in a mouse model of postinfectious bowel dysfunction. | patients with postinfective irritable bowel syndrome and trichinella spiralis-infected mice share many features including visceral hypersensitivity and disordered motility. we assessed enterochromaffin (ec) numbers and serotonin transporter (sert) using national institute of health (nih) female mice studied for up to 56 days post-t. spiralis infection. the effects of steroid treatment and the t-cell dependence of the observed responses were assessed by infection of hydrocortisone-treated or t-ce ... | 2005 | 16336502 |
| [detection of anti-trichinella spiralis antibody by indirect elisa using recombinant protein as antigen]. | to study the specificity, sensitivity of diagnostic antigen for trichinella spiralis (t.s). | 2005 | 16300001 |
| [effect of the culture fluid of trichinella spiralis on the apoptosis and necrosis of human leukaemia k562 cells]. | 2005 | 16300018 | |
| what is the role of p53 during the cyst formation of trichinella spiralis ? a comparable study between knockout mice and wild type mice. | during the cyst formation of trichinella spiralis, the infected muscle cell undergoes basophilic change and apoptosis, which results in nurse cell formation. this study revealed expression kinetics of some apoptosis genes such as p53 and its closely related genes (tumor suppressor genes p53, p53; mouse double minute 2, mdm2; cyclin-dependent kinase inhibitor p21, p21waf). rt-pcr (reverse transcription polymerase chain reaction) results showed that these genes were temporarily expressed in the in ... | 2005 | 16255829 |
| [preparation and preliminary application of monoclonal antibodies against adult worm of angiostrongylus cantonensis]. | to develop and identify monoclonal antibodies (mcabs) against adult worm of angiostrongylus cantonensis and observe its applicability. | 2005 | 16296607 |
| [construction and expression of the ts87 gene of trichinella spiralis in pichia pastoris]. | to construct the recombinant plasmid p(picz) alphaa-ts87 and obtain secretive protein in pichia pastoris. | 2005 | 16296617 |
| calcification of trichinella spiralis larval capsule. | trichinella spiralis larvae were examined in tem to identify calcareous corpuscles in the outer part of parasite capsule. the microroentgenographic analysis of calcareous corpuscles mainly demonstrated the presence of phosphorus and calcium. the physiological importance of calcareous corpuscles, as well as their significance in decay of t. spiralis larvae were discussed. | 2005 | 16211414 |
| proteomic analysis of the excretory-secretory proteins of the trichinella spiralis l1 larva, a nematode parasite of skeletal muscle. | trichinella spiralis is an intracellular nematode parasite of mammalian skeletal muscle. infection of the muscle cell leads to the formation of a host-parasite complex that results in profound alterations to the host cell and a re-alignment of muscle-specific gene expression. the role of parasite excretory-secretory (es) proteins in mediating these effects is currently unknown, largely due to the difficulty in identifying and assigning function to individual proteins. in this study, a global pro ... | 2005 | 16220533 |
| tumor necrosis factor receptor-mediated apoptosis in trichinella spiralis-infected muscle cells. | in order to reveal the mechanisms underlying nurse cell formation during trichinella spiralis infection, the expression of the factors of tumor necrosis factor-alpha (tnf-alpha)/tnf receptor 1 (tnfr-1) signalling pathway mediating apoptosis was investigated. the analysed factors included tnf-alpha, tnfr-1, tnf receptor-associated death-domain (tradd), caspase 3, caspase 8, tnf receptor associated factor-2 (traf2) and receptor interactive protein (rip), all of which are involved in the tnf-alpha/ ... | 2005 | 16178359 |
| effect of aminoguanidine and albendazole on inducible nitric oxide synthase (inos) activity in t. spiralis-infected mice muscles. | the aim of this study was to provide evidence for the expression of inos in the cells of inflammatory infiltrates around larvae in skeletal muscles of t. spiralis infected mice. the balb/c mice (n = 8) divided into subgroups, received either aminoguanidine (amg)--a specific inos inhibitor or albendazole (alb)--an antiparasitic drug of choice in trichinellosis treatment. control animals (n = 2 in each subgroup) were either uninfected and treated or uninfected and untreated. frozen sections of hin ... | 2005 | 16201316 |
| reduction in the number of infective trichinella spiralis larvae in mice by use of homeopathic drugs. | trichinellosis caused by the gastrointestinal nematode trichinella spiralis occurs in humans, domestic animals and wild animals. it is difficult to control the muscle phase of the parasite. homeopathic drugs such as cina and santoninum have anthelmintic properties. we have observed that in material doses, the homeopathic drug podophyllum also has nematotoxic properties. we have also observed that homeopathic potency can influence the water permeability of cells. | 2005 | 16137982 |
| developmental arrest in caenorhabditis elegans dauer larvae causes high expression of enzymes involved in thymidylate biosynthesis, similar to that found in trichinella muscle larvae. | crude extract specific activities of thymidylate synthase, dutpase, thymidine kinase and dihydrofolate reductase were high during the development of caenorhabditis elegans, the dauer larva activities being similar to those previously determined in trichinella spiralis and t. pseudospiralis muscle larvae (with the exception of thymidine kinase, not detected in trichinella). high thymidylate synthase expression in developmentally arrested larvae, demonstrated also at the mrna and protein levels, i ... | 2005 | 16145941 |
| in vitro and in vivo effects of progesterone on trichinella spiralis newborn larvae. | we have previously demonstrated that during pregnancy there exists an increased parasiticide activity against trichinella spiralis newborn larvae (nbl) in infected rats. in this work we analysed the contribution of peritoneal cells from noninfected pregnant rats to the mortality of the nbl in cytotoxicity assays, and evaluated the role of progesterone in this effector mechanism. our findings suggest that progesterone can induce activation of effector peritoneal cells to destroy the nbl in a rapi ... | 2005 | 16145942 |
| trichinella spiralis: enteric mucin-related response to experimental infection in conventional and spf pigs. | duodenal and jejunal responses to infection with trichinella spiralis were compared in weaned piglets with a "normal dirty" vs. a "clean spf" gut flora. histochemical staining of neutral, acidic, sialylated, and sulphated residues was used to assess biosynthetic responses in mucin-secreting goblet cells. peanut and ulex lectins were also used to assess responses within the intestinal glycocalyx. histomorphometric analysis was undertaken to evaluate the distribution and staining patterns of goble ... | 2005 | 15687012 |
| [current knowledge on human trichinellosis]. | until the beginning of the nineties, the control of trichinellosis was thought to be a solved problem in europe. however, as a consequence of social, economical and ecological changes in central eastern and eastern europe in the past decade, trichinellosis became a re-emerging infectious disease in the region. molecular genetic studies demonstrated that at least two trichinella species can be responsible for trichinellosis outbreaks in the region, and the epidemiology of trichinellosis is more c ... | 2005 | 15693443 |
| inhibition of mcp-1 and mip-2 chemokines in murine trichinellosis: effect of the anti-inflammatory compound l-mimosine. | mimosine is a plant amino-acid which has been reported to block dna replication in mammalian cells and to arrest cell reversibly towards the end of the g1 phase or at the beginning of the s phase. in this study, 42 mice were infected with t. spiralis a nematode parasite, and treated with the anti-inflammatory compound l-mimosine, to determine if any alteration in the chronic inflammatory state occurred, by investigating the hosts immunological response. mcp-1, a c-c chemokine and mip-2, a c-x-c ... | 2005 | 15698514 |
| trichinella spiralis: affinity purified antigen based diagnosis and immunoprophylaxis. | the current research introduces a trial to develop vaccine candidate against trichenosis. a method of affinity chromatography was adopted to purify a trichinella spiralis larval extract. the isolated fraction resolved into six bands of 148 kda, 133 kda, 118.5 kda, 101 kda 98.5 kda and 79.5 kda as observed by sds-page. the diagnostic value of this fraction was checked against antibodies regularly collected from rats experimentally infected with trichinosis compared with that of crude larval extra ... | 2005 | 16083053 |
| congenital trichinellosis in experimentally infected mice. | the present study aimed at allocating the time during pregnancy at which transplacental transmission of trichinella spiralis (t. spiralis) larvae took place. swiss albino mice were infected at different durations of pregnancy; five days before mating, on gestation day zero and five days after mating. furthermore, to study the effect of immunosuppression on transplacental transmission, half of the experimental mice were immunosuppressed using cyclophosphamide drug (endoxan). the percentage of inf ... | 2005 | 16083057 |
| identification and diagnostic evaluation of cross-reactive antigen between hydatid cyst fluid of echinococcus granulosus and trichinella spiralis larval extract. | a cross reactive fraction was isolated from hydatid cyst fluid antigen of e. granulosus using cnbr sepharose 4b affinity chromatography in which anti-t. spiralis antibodies were coupled with the column. biochemical characterization of the isolated fraction included the use of sds-page, isoelectric focusing and amino acid analysis. the fraction showed 5 polypeptides of 165, 95.5, 63.5, 30.6 and 24 kda. the isoelectric points of these polypeptides were 7.8, 7.2, 6.7, 6.2 and 5.7. the fraction exhi ... | 2005 | 16083063 |
| effect of nigella sativa and allium cepa oils on trichinella spiralis in experimentally infected rats. | prophylactic and therapeutic effect of two oils had been carried out either prior to infection or postinfection respectively in rats. each rat in either case was orally administered with n. sativa oil or a. cepa oil in a dose 5 mg/kg body weight/day for 2 weeks. assessment of results was by: (1) adult worm count in the intestine on 7th and 20th day post infection. (2) larval count in the muscles on the 60th day post infection. (3) index of reproductive capacity. (4) detection of antibodies again ... | 2005 | 16083064 |
| synthesis and antitrichinellosis activity of some 2-substituted-[1,3]thiazolo[3,2-a]benzimidazol-3(2h)-ones. | some new thiazolo[3,2-a]benzimidazolone derivatives were synthesized using two methods. the structures of the synthesized compounds were proved by means of ir, (1)h nmr and mass spectral data. ab initio computations were performed in order to determine the electronic structure and geometry of the investigated molecules and to compare it to the geometry of albendazole. biologically, experiments in vitro and in vivo were accomplished in order to identify the efficacy of the obtained thiazolobenzim ... | 2005 | 16084100 |
| characterization of functional and morphological changes in a rat model of colitis induced by trichinella spiralis. | we intended to characterize the effect of inflammation on the spontaneous colonic motility pattern and the role of inos in its disruption in colitis. colitis was induced by an intracolonic enema of t. spiralis larvae. animals were studied 2-30 days postinfection (pi). standard h&e and inos staining was performed on colonic sections. altered stool consistency was found from day 1 to day 21 pi; leukocytosis peaked on days 6-21 pi. edema and cell infiltration were found in mucosa and submucosa (day ... | 2005 | 16110832 |
| intraepithelial nk cell-derived il-13 induces intestinal pathology associated with nematode infection. | il-13 is a th2-derived cytokine associated with pathological changes in asthma and ulcerative colitis. moreover, it plays a major role in the control of gut nematode infection and associated immunopathology. the current paradigm is that these effects are due to t cell-derived il-13. we show in this study that an innate source of il-13, the intraepithelial nk cell, is responsible for the disruption of intestinal tissue architecture and induction of goblet cell hyperplasia that characterizes infec ... | 2005 | 16116211 |
| helminth regulation of host il-4ralpha/stat6 signaling: mechanism underlying nos-2 inhibition by trichinella spiralis. | gastrointestinal nematode infection is known to alter host t cell activation and has been used to study immune and inflammatory reactions in which nitric oxide (no) is a versatile player. we previously demonstrated that trichinella spiralis infection inhibits host inducible no synthase (nos-2) expression. we now demonstrate that (i) an il-4 receptor alpha-subunit (il-4ralpha)/stat6-dependent but t cell-independent pathway is the key for the nematode-induced host nos-2 inhibition; (ii) endogenous ... | 2005 | 15741272 |
| ige: a question of protective immunity in trichinella spiralis infection. | the best-known function of immunoglobulin e (ige) is the initiation of allergic reaction. however, it does not make sense that ige exists only for allergy, which is harmful for the body and does not have a beneficial role. in this article, we focus on ige in trichinella spiralis infection in rodents and humans, and discuss the possible roles of ige in protection and pathogenesis. | 2005 | 15780839 |
| nematocidal and trichomonacidal activities of 2-substituted quinolines. | several quinolines were synthesized and evaluated in vitro and in vivo against the nematodes caenorhabditis elegans, heligmosomoides polygyrus and the protozoa trichomonas vaginalis. if some of them have shown in vitro nematocide activity (at 10 microm), however, their trichomonacidal activity reached 50% reduction at only 100 microm. the in vivo activity on trichinella spiralis model was evaluated for some of the most in vitro active quinolines. | 2005 | 15784240 |
| [trichinosis in the republic of north ossetia-alania]. | the present paper presents the biological, epidemiological, clinical, and immunological characteristics of trichinosis in the republic of north ossetia-alania, which is based on the results of long-term studies and follow-ups and on the 1971-2002 report data from medical and veterinary services and hunt inspectorate agencies. it has established that a total of 675 clinical cases of trichinosis were recorded in ossetia-alania in the period under study. the incidence rate of the disease ranged fro ... | 2005 | 15801214 |
| [investigation of the trichinocidal activity of the agent vig-03]. | 2005 | 15801220 | |
| trichinella spiralis and laryngeal carcinoma: a case report. | an uncommon presentation of trichinella spiralis with squamous cell carcinoma of the larynx is reported. the possible carcinogenic potential of trichinosis and its management are discussed. | 2005 | 15549336 |
| rediscovery of trichinella spiralis in red foxes (vulpes vulpes) in ireland after 30 years of oblivion. | to determine whether or not ireland can be considered as trichinella-free, after more than 30 years of no reported infections in domestic and sylvatic animals and in humans. | 2005 | 15603842 |
| parasite nucleotide-metabolizing enzymes and host purinergic signalling. | tissue damage results in a variety of molecular signals that activate elements of the immune system. recent years have seen a growing awareness that key regulators of these events are extracellular nucleotides that signal through purinergic receptors. haematophagous insects and ticks secrete enzymes in their saliva that degrade nucleotides, thus inhibiting haemostasis and minimizing the ensuing pain and inflammatory reactions provoked by these mediators. the discovery of an enzymatic cascade of ... | 2005 | 15639736 |
| disruption of cd40-cd40 ligand pathway inhibits the development of intestinal muscle hypercontractility and protective immunity in nematode infection. | in our previous studies, we demonstrated that during trichinella spiralis infection, t helper (th) 2 cells contribute to the development of intestinal muscle hypercontractility and worm expulsion from the gut via stat6. in addition, we have linked the altered muscle contractility to the eviction of the parasite and thereby to the host defense. however, the initial events linking infection to the development of muscle hypercontractility are poorly understood. in this study, we examined the contri ... | 2005 | 15308470 |
| detection of paragonimus heterotremus eggs in experimentally infected cats by a polymerase chain reaction-based method. | a polymerase chain reaction (pcr) procedure for the detection of paragonimus heterotremus eggs in stool samples was developed and compared with stoll's egg count method. the primers were designed on the basis of a previously constructed pph-13-specific dna probe, which produced an approximate 0.5-kb amplified product. this pcr method could detect as few as 5 eggs in 0.6 g of artificially inoculated feces of a healthy control cat or as little as 1 x 10(-4) ng of p. heterotremus genomic dna. the a ... | 2005 | 15856902 |
| spatial variation of trichinella prevalence in rats in finnish waste disposal sites. | trichinellosis is 1 of the most widespread parasitic zoonoses in the world and can be lethal to humans. trichinella spp. are also parasites of considerable economic importance. because rats may play a role in the transmission of trichinellosis to swine and farmed wild boar, 767 brown rats (rattus norvegicus berkenhout) from 13 finnish waste disposal sites were examined for trichinella spp. by a hcl-pepsin digestion method. trichinella spp. were found to be a common parasite in trapped rats (over ... | 2005 | 15856908 |
| first record of human trichinosis in chile associated with consumption of wild boar (sus scrofa). | the first south american case of human trichinosis, resulting from the consumption of roast wild boar (sus scrofa) is reported in chile. the patient presented fever, diarrhea, myalgias, facial edema, sub-conjunctival reddening, photophobia, eosinophilia, and elevated glutamic oxalacetic transaminase. the diagnosis was confirmed by two immunoenzymatic tests (elisa) using somatic and excretion-secretion antigens. | 2005 | 15867957 |
| serological diagnosis of trichinella spiralis in experimentally infected mice. | elisa and ifa tests were used to detect igg levels in sera of experimentally infected mice with trichinella spiralis, during a period of 12 weeks post infection. a crude saline extract of muscle larvae was used for application of elisa technique, while the intact t. spiralis muscle larvae were used as an antigen for ifat. in both tests, t. spiralis igg antibodies could be detected one week post infection in sera of all infected mice. elisa test showed a sensitivity of 100% during the whole perio ... | 2005 | 15881000 |
| cross-protection induced by cross-reactive antigen against fasciola gigantica and trichinella spiralis infections. | trichinella spiralis /fasciola gigantica cross-reactive fraction was purified from t. spiralis larval extract by affinity column chromatography in which cnbr-sepharose 4b was coupled with f. gigantica antibodies. the fraction consisted of six polypeptides of 191kda, 178kda, 149kda, 106kda, 101kda and 32 kda as revealed by sds-page. analysis of the free amino acids of the fraction revealed 17 amino acids with high proportions of tyrosine and glutamic. immunization of rabbits subcutaneously with t ... | 2005 | 15881013 |
| binding and repression of translation of the cognate mrna by trichinella spiralis thymidylate synthase differ from the corresponding interactions of the human enzyme. | thymidylate synthase (ts) of trichinella spiralis, a parasitic nematode causing trichinellosis, was found to bind its own mrna and repress translation of the latter, similar to its human counter-part [chu, koeller, casey, drake, chabner, elwood, zinn and allegra (1991) proc. natl. acad. sci. u.s.a. 88, 8977-8981]. however, in striking contrast with human ts, the parasite enzyme's interaction with mrna was not affected by any of the substrate (deoxyuridylate or n(5,10)-methylenetetrahydrofolate) ... | 2005 | 15882146 |
| will corticosteroids and other anti-inflammatory agents be effective for diarrhea-predominant irritable bowel syndrome? | irritable bowel syndrome (ibs) is one of several functional gastrointestinal disorders commonly encountered in both the clinical setting and the general population. the biopsychosocial model is currently believed to be a more complete explanatory mechanism of ibs symptom genesis and propagation. gut inflammation and immune activation is one of the biological mechanisms for which evidence is emerging. experimental parasitic infection of mice bowel resulted in elevated substance p levels and incre ... | 2005 | 15893125 |
| contrasting effects of acute and chronic gastro-intestinal helminth infections on a heterologous immune response in a transgenic adoptive transfer model. | we have previously found that co-immunisation with ovalbumin (ova) and the body fluid of the helminth ascaris suum inhibited an ova-specific delayed type hypersensitivity (dth) response by reducing ova-specific cd4+ t lymphocyte proliferation via an il-4 independent mechanism. in the present study, we determined whether parasite infections themselves could induce similar changes to peripheral immunisation by examining the modulation of ova-specific immune responses during acute and chronic helmi ... | 2005 | 15893319 |
| establishment and characterization of continuous hematopoietic progenitors-derived pig normal mast cell lines. | mast cells (mcs) are tissue resident, hematopoietic stem cells-derived elements, distributed throughout the body. they are the pivotal mediating cells of allergic reactions. in addition, in mice, mcs play a critical role in the defense against several pathogens, such as bacteria, parasites and viruses. whereas the biology of rodent and human mcs has been extensively studied using in vitro derived populations, the role of mcs in pigs has not yet been evaluated, given the very low availability of ... | 2005 | 15896811 |
| endogenous morphine and parasitic helminthes. | the current paper summarizes the findings on endogenous morphine isolated by hplc and characterized by mass spectroscopy in schistosoma mansoni, dracunculus medinensis and ascaris suum. morphine-6-glucuronide (m6g) has also been found by hplc and confirmed by mass spectroscopy in dracunculus medinensis and ascaris suum. in addition, a morphine like substance has been isolated from trichinella spiralis by hplc and mice infected with trichinella spiralis show a naloxone reversible analgesia. we di ... | 2005 | 15917729 |
| [trichinosis infection--increasing again in eastern europe and balkan]. | 2005 | 15921107 | |
| intestinal establishment and reproduction of adult trichinella spp. in single and mixed species infections in foxes (vulpes vulpes). | intestinal establishment and reproduction of adult trichinella spiralis, trichinella nativa, trichinella britovi and trichinella pseudospiralis were examined as single species or mixed species infections in foxes. this is the first study of intestinal dynamics of trichinella spp. in a carnivore model and the results suggest that the intestinal phase is relatively short as only very few worms were recovered 10 days post-inoculation (dpi). in mixed species infection with equal doses of t. nativa a ... | 2005 | 15925724 |
| studies on vertical transmission of trichinella spp. in experimentally infected ferrets (mustela putorius furo), foxes (vulpes vulpes), pigs, guinea pigs and mice. | vertical transmission of trichinella spiralis was evaluated in ferrets (n=21), foxes (n=11), pigs (n=12), guinea pigs (n=16), and mice (n=41). the placental barrier to be crossed by migratory trichinella larvae varies structurally in different animal species. ferrets and foxes have an endotheliochorial placenta structure, guinea pigs and mice a haemochorial, and pigs an epitheliochorial placenta. the non-encapsulating trichinella pseudospiralis larvae have an extended muscle migration prior to e ... | 2005 | 15925725 |
| molecular cloning and functional expression of enolase from trichinella spiralis. | the cdna library was constructed from muscle larvae of trichinella spiralis, and immunoscreened with sera from mice infected with t. spiralis. a cdna clone, designated as tseno, encoded 2-phospho-d-glycerate hydro-lyase (enolase) that catalyzed a reversible conversion of 2-phospho-d-glyceric acid (2pga) to phosphoenolpyruvate (pep) in the glycolytic pathway. the recombinant tseno protein was produced in an escherichia coli expression system. the recombinant full-length tseno protein had no activ ... | 2005 | 15928906 |
| trichinella pseudospiralis infection is characterized by more continuous and diffuse myopathy than t. spiralis infection. | a time course study was performed to reveal the sequence of histopathology after trichinella spiralis or t. pseudospiralis infection in mice. a cyst was formed in the former case by about 18 days post infection and prominent myopathy was restricted within the cyst. in the latter case, however, no typical cyst was formed, and myopathy spread diffusely over the infected muscle tissues occupying half the area of muscle sections. an electron microscope observation revealed that the disintegration of ... | 2005 | 15948011 |
| electrocardiographic changes and myocarditis in trichinellosis: a retrospective study of 154 patients. | the frequencies of electrocardiographic (ecg) abnormalities and myocarditis were determined, retrospectively, among 154 cases of trichinellosis [101 males and 53 females, with a mean (s.d.) age of 35.60 (14.64) years] who were hospitalized at the university hospital for infectious diseases in zagreb, croatia, over a 5-year period. eighty-seven (56%) of the patients, most of them in the invasive phase of infection with trichinella spiralis, were found to have abnormalities when examined by 12-lea ... | 2005 | 15949188 |
| trichinella britovi etiological agent of sylvatic trichinellosis in the republic of guinea (west africa) and a re-evaluation of geographical distribution for encapsulated species in africa. | in west africa, trichinella infection was documented in humans and animals from senegal in the 1960s, and the biological characters of one isolate showed a lower infectivity to domestic pigs and rodents when compared with that of a trichinella spiralis pig isolate from europe. to identify the trichinella species present in west africa, a survey was conducted in a total of 160 wild animals in the republic of guinea. three viverridae, one true civet (viverra civetta) and two african palm civets (n ... | 2005 | 15964575 |
| survey on porcine trichinellosis in ecuador. | a survey on porcine trichinellosis was organised in ecuador between 2000 and 2003. blood samples were taken in slaughterhouses (study 1, n=2000; study 2, n=331) and in a remote village where pigs are free roaming (study 3, n=646) and examined by elisa using excretory/secretory (e/s) antigens. seven samples (0.35%) in study 1 and none of the samples of study 2 were serologically positive. thirty-seven (5.72%) village pigs tested positive by e/s elisa in study 3. sero-positive results by the e/s e ... | 2005 | 15978724 |
| serological evidence of trichinellosis in local pigs of nepal. | in nepal, animal husbandry is a major source of income. pig husbandry is practiced in rural, peri-urban, and urban communities. free ranging "back yard" pigs and the practice of feeding offal is a very common management practice which potentially allows for the transmission of trichinellosis; however, this zoonosis has never been reported from this region. a total of 425 serum samples were collected from local pigs. these were initially screened by elisa after which positive samples were examine ... | 2005 | 15978729 |
| muscle distribution of sylvatic and domestic trichinella larvae in production animals and wildlife. | only a few studies have compared the muscle distribution of the different trichinella genotypes. in this study, data were obtained from a series of experimental infections in pigs, wild boars, foxes and horses, with the aim of evaluating the predilection sites of nine well-defined genotypes of trichinella. necropsy was performed at 5, 10, 20 and 40 weeks post inoculation. from all host species, corresponding muscles/muscle groups were examined by artificial digestion. in foxes where all trichine ... | 2005 | 15979801 |
| comparison of two antigens for demonstration of trichinella spp. antibodies in blood and muscle fluid of foxes, pigs and wild boars. | for the surveillance of trichinellosis, the digestion method is reliable but also labour intensive. the serological methods for the detection of trichinella-specific antibodies using elisa offer a sensitive and relatively specific alternative. for serological studies, sera or plasma from blood samples are the most common source of antibodies, but although the concentration of antibodies is approximately 10-fold lower, muscle fluid can be a good alternative particularly for testing of wildlife sa ... | 2005 | 15982820 |
| [preparation of a dry red blood cell trichinella antigenic diagnosticum for indirect hemagglutination test and evaluation of its efficiency]. | the present paper presents the stages of a process for manufacturing a dry red blood cell trichinella antigenic diagnosticum for indirect hemagglutination test (ihat) and the evaluation of its diagnostic efficiency. the diagnosticum is shown to be a 3% suspension lyophilized from formalinized and tanned sheep red blood cells on which an excretory-secretory antigen of invasion trichinella larvae was absorbed in a dose of 100 mg/ml. the rather high sensitivity (98.7%) and specificity (97.3%) of ih ... | 2005 | 15984615 |
| experimental studies in pigs on trichinella detection in different diagnostic matrices. | a total of 72 specific pathogen-free (spf) and iberian pigs (three animals per group) were inoculated with 200, 1000 or 20,000 muscle larvae of t. spiralis, t. nativa, t. britovi and t. pseudospiralis. for each animal, the muscle larva burden was evaluated in nine muscle samples by digestion. the anti-trichinella igg kinetics in blood samples, taken twice prior and at days 5, 10, 15, 20, 25, 30, 40, 50 and 60 post-inoculation, and in muscle juice, obtained at necropsy, was evaluated by an elisa ... | 2005 | 15985334 |
| molecular cloning of a cdna encoding a putative cuticle collagen of trichinella spiralis. | a 5-day-old adult stage-specific cdna fragment from trichinella spiralis was identified by suppression subtractive hybridization and was used as a probe to screen the cdna library. the cdna sequence coding for a putative t. spiralis cuticle collagen was isolated. the cdna encoded an open reading frame of 343 amino acid residues with molecular weight of 35.1 k da. the deduced protein contained an n-terminal signal peptide, a nematode cuticle collagen n-terminal domain and a collagen triple helix ... | 2005 | 15985335 |
| comparative dynamics and phenotype of the murine immune response to trichinella spiralis and trichinella pseudospiralis. | infection of nih mice with trichinella spiralis and trichinella pseudospiralis results in qualitatively comparable immune responses. antigen-specific proliferation by mesenteric lymph node cells was transient and temporally associated with intestinal infection, but in contrast was sustained throughout infection by splenocytes. early cytokine production by mesenteric lymph node cells was dominated by interleukin 10, but also il-5 and il-4, with rapid resolution following parasite expulsion from t ... | 2005 | 15987341 |
| re-evaluation of patients involved in a trichinellosis outbreak caused by trichinella britovi 15 years after infection. | this study re-evaluates 13 out of 48 subjects involved in a trichinellosis outbreak that occurred in central italy (umbria region) in 1988 resulting from the consumption of raw boar meat harboring trichinella britovi. during the outbreak, 28 of 48 serologically positive subjects were asymptomatic, whereas 20 subjects presented one or more clinical signs including but not limited to fever, myalgia, periorbital oedema and conjunctivitis. several patients were hospitalized with severe clinical sign ... | 2005 | 15990231 |
| influence of adjuvant formulation on the induced protection of mice immunized with total soluble antigen of trichinella spiralis. | vaccination of pigs against the helminth nematode trichinella could be a good alternative to prevent the risk of human infection. in order to develop an efficient and safe vaccine, the choice of the adjuvant is an important issue. in this study, two adjuvants were selected to prepare vaccines based on total soluble trichinella spiralis muscle larvae (ml) antigen: montanide isa 70 water in oil emulsion and montanide ims nanoparticles. aluminium hydroxide was used as a reference adjuvant. the immu ... | 2005 | 15990233 |
| a spectrum of functional genes mobilized after trichinella spiralis infection in skeletal muscle. | trichinella spiralis infection causes the transformation of infected muscle cells, which leads to nurse cell formation. to search for the candidate genes responsible for nurse cell formation, cdna microarray analysis of muscle tissues was performed before and after trichinella infection. the atlas mouse 1.2 cdna expression microarray revealed the expression profiles of 1176 known genes. out of these, 311 gene expressions were detected in normal and/or infected muscles. after the infection, 184 o ... | 2005 | 15991499 |
| a stage-specific open reading frame from three-day old adult worms of trichinella spiralis encodes zinc-finger motifs. | the aim of the study was to isolate genes coding for stage-specific antigens of t. spiralis. such antigens may then be associated with local and systemic immune responses against adult t. spiralis. recombinant clones were obtained with an adult stage specific probe from a cdna library of three-day old adult t. spiralis. several cdna clones encoding the same peptide were identified and their stage specificity was confirmed by northern blot analysis. three independent clones were fully sequenced, ... | 2005 | 15991828 |
| anti-trichinella antibodies detected in chronically infected horses by ifa and western blot, but not by elisa. | in the balkan countries, where trichinellosis is a re-emerging zoonosis, it is of great importance to determine trichinella infection prevalence among the major hosts, including horses. one method for monitoring prevalence is serological surveillance; however, the validity of serological methods in horses is not well understood. the dynamics of anti-trichinella igg production and circulating excretory/secretory (es) antigens were investigated in three horses experimentally-infected with trichine ... | 2005 | 15992997 |
| simple species identification of trichinella isolates by amplification and sequencing of the 5s ribosomal dna intergenic spacer region. | we developed a pcr-based assay using a single primer pair to amplify the 5s ribosomal dna intergenic spacer region to identify trichinella isolates. in our method, amplified products are directly sequenced on both strands and compared to genbank sequences. using this method, we were able to identify trichinella spiralis, t. britovi and t. nativa. this method permits rapid species identification of trichinella isolates; however, further evaluation is required before recommending this approach for ... | 2005 | 15992998 |
| profiling excretory/secretory proteins of trichinella spiralis muscle larvae by two-dimensional gel electrophoresis and mass spectrometry. | infection of mammalian skeletal muscle with the intracellular parasite trichinella spiralis results in profound alterations in the host cell and a realignment of host cell gene expression. the role of parasite excretory/secretory (e/s) products in mediating these effects is unknown, largely due to the difficulty in identifying and assigning function to individual proteins. in this study, we have used two-dimensional electrophoresis to analyse the profile of muscle larva excreted/secreted protein ... | 2005 | 15992999 |
| trichinae certification in the united states pork industry. | control of trichinella infection in u.s. pork has traditionally been accomplished by inspection of individual carcasses at slaughter or by post-slaughter processing to inactivate parasites. we propose that an alternative to individual carcass testing or processing can be used when pigs are raised in production systems where risk of exposure to trichinella spiralis has been mitigated. declines in prevalence of this parasite in u.s. domestic swine during the last 30 years, coupled with improvement ... | 2005 | 15993000 |
| expressed sequence tags from life cycle stages of trichinella spiralis: application to biology and parasite control. | while the approach taken to date to study trichinella spp., involves mainly characterization of individual genes of interest, we initiated a genomics approach as an antecedent to more complete genome sequencing. our approach involves use of expressed sequence tags (ests) obtained from three life cycle stages of trichinella spiralis; adult worms (ad), mature muscle larvae (ml) and immature l1 larvae (imml1, also known as newborn larvae) () to improve the technical capacity for research on trichin ... | 2005 | 15993542 |
| evaluation of elisa for detection of trichinella antibodies in muscle juice samples of naturally infected pigs. | the performance characteristics of an elisa test for trichinellosis in pigs applied to muscle juice was assessed using 314 samples collected from pigs located in endemic areas of croatia. peptic digestion was used as the reference method. the diagnostic accuracy of the two compared dilutions (1:10 and 1:100) was considered to be high because the area under the curve (auc) index was 0.922 and 0.920 for each dilution, respectively. in this study the two graph-receiver operating characteristic (tg- ... | 2005 | 15993544 |
| the prevalence of trichinella infection in domestic dogs in finland. | in this study, we examined the prevalence of trichinella infection in domestic dogs in finland, which is known to have a high prevalence of trichinella infection in sylvatic hosts. altogether 727 dog serum samples were tested serologically by elisa with es-antigen in dilutions 1:50, 1:100, 1:200, and 1:400. additionally, muscle samples from 102 dogs' front leg extensors were tested parasitologically by hcl-pepsin-digestion. the tested dogs originated from the university veterinary hospital (244 ... | 2005 | 15994010 |