Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
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| analysis of genes developmentally regulated during storage root formation of sweet potato. | to identify the genes involved in storage root formation of sweet potato (ipomoea batatas), we performed a simplified differential display analysis on adventitious roots at different developmental stages of the storage root. the expression patterns were confirmed by semiquantitative rt-pcr analyses. as a result, 10 genes were identified as being developmentally regulated and were named srf1-srf10. the expression of srf1, srf2, srf3, srf5, srf6, srf7, and srf9 increased during storage root format ... | 2005 | 15700424 |
| palm tree peroxidase-based biosensor with unique characteristics for hydrogen peroxide monitoring. | three amperometric enzyme electrodes have been constructed by adsorbing anionic royal palm tree peroxidase (rptp), anionic sweet potato peroxidase (spp), or cationic horseradish peroxidase (hrp-c) on spectroscopic graphite electrodes. the resulting h(2)o(2)-sensitive biosensors were characterized both in a flow injection system and in batch mode to evaluate their main bioelectrochemical parameters, such as ph dependency, i(max), k(m)(app), detection limit, linear range, operational and storage s ... | 2005 | 16242613 |
| unravelling the genetic diversity of the three main viruses involved in sweet potato virus disease (spvd), and its practical implications. | summary sweetpotato (ipomoea batatas) is a widely grown food crop, in which the most important diseases are caused by viruses. genetic variability of three widely distributed sweetpotato viruses was analysed using data from 46 isolates of sweet potato feathery mottle virus (spfmv), 16 isolates of sweet potato mild mottle virus (spmmv) and 25 isolates of sweet potato chlorotic stunt virus (spcsv), of which 19, seven and six isolates, respectively, are newly characterized. division of spfmv into f ... | 2005 | 20565651 |
| overview and analysis of the polyprotein cleavage sites in the family potyviridae. | summary the genomes of plant viruses in the family potyviridae encode large polyproteins that are cut by virus-encoded proteases into ten mature proteins. three different types of protease have been identified, each of which cuts at sites with a distinctive sequence pattern. the experimental evidence for this specificity is reviewed and the cleavage site patterns are compiled for all sequenced species within the family. seven of the nine cleavage sites in each species are cut by the viral nia-pr ... | 2005 | 20565672 |
| analysis of a sugar response mutant of arabidopsis identified a novel b3 domain protein that functions as an active transcriptional repressor. | a recessive mutation hsi2 of arabidopsis (arabidopsis thaliana) expressing luciferase (luc) under control of a short promoter derived from a sweet potato (ipomoea batatas) sporamin gene (spo(min)luc) caused enhanced luc expression under both low- and high-sugar conditions, which was not due to increased level of abscisic acid. the hsi2 mutant contained a nonsense mutation in a gene encoding a protein with b3 dna-binding domain. hsi2 and two other arabidopsis proteins appear to constitute a novel ... | 2005 | 15894743 |
| toxicity of novel sequiterpenoids from the stressed sweet potato (ipomoea batatas). | 2004 | 520970 | |
| molecular cloning of a cytosolic ascorbate peroxidase cdna from cell cultures of sweet potato and its expression in response to stress. | a cdna encoding a cytosolic ascorbate peroxidase (apx), swapx1, was isolated from cell cultures of sweet potato ( ipomoea batatas) by cdna library screening, and its expression in the context of various environmental stresses was investigated. swapx1 contains an orf of 250 amino acids (27.5 kda) encoding a protein with a pi value of 5.32. the swapx1 orf does not code for a transit peptide, suggesting that the product is a cytosolic isoform. rna blot analysis showed that swapx1 gene is expressed ... | 2004 | 14986108 |
| molecular cloning and characterization of a cdna encoding asparaginyl endopeptidase from sweet potato (ipomoea batatas (l.) lam) senescent leaves. | asparaginyl endopeptidase is a cysteine endopeptidase that has strict substrate specificity toward the carboxyl side of asparagine residues, and is possibly involved in the post-translational processing of proproteins. in this report one full-length cdna, spae, was isolated from senescent leaves of sweet potato (ipomoea batatas (l.) lam). spae contained 1479 nucleotides (492 amino acids) in the open reading frame, and exhibited high amino acid sequence homologies (c. 61-68%) with asparaginyl end ... | 2004 | 14990624 |
| control strategies for sweet potato virus disease in africa. | sweet potato virus disease (spvd), caused by dual infection with the whitefly-borne sweet potato chlorotic stunt virus (spcsv) and the aphid-borne sweet potato feathery mottle virus (spfmv), is the most serious disease of sweet potato in africa. spvd has been known there since at least the 1940s, although it took several decades to elucidate its aetiology. it occurs throughout africa and is particularly prevalent in the great lakes region. production of sweet potato is largely by resource-poor f ... | 2004 | 15036842 |
| whitefly transmission of sweet potato viruses. | three genera of plant viruses, begomovirus (geminiviridae), crinivirus (closteroviridae) and ipomovirus (potyviridae), contain members that infect sweet potato (ipomoea batatas) and are transmitted by whiteflies. the begomoviruses, sweet potato leaf curl virus (splcv) and ipomoea leaf curl virus (ilcv), and the ipomovirus sweet potato mild mottle virus are transmitted by bemisia tabaci, the sweet potato whitefly. the crinivirus, sweet potato chlorotic stunt virus (spcsv), is transmitted by b. ta ... | 2004 | 15036843 |
| in vitro evaluation of physiological activity of vinegar produced from barley-, sweet potato-, and rice-shochu post-distillation slurry. | vinegar was produced from barley-, sweet potato-, and rice-shochu post-distillation slurry using jar fermentor within 19 hrs. all the vinegars showed radical-scavenging activity, angiotensin i converting enzyme (ace) inhibition and advanced glycation endproducts (age) inhibition in vitro. the radical-scavenging activity of the vinegar produced from sweet potato-shochu post-distillation slurry was higher than that of other two kinds of vinegar on the organic matter basis. the ace inhibitory activ ... | 2004 | 15056886 |
| effect of preozonation on improvement of settleability of solid in highly concentrated organic wastewater of japanese wheat and sweet potato spirit-distillery. | solid-liquid separation of the wastewater is very difficult because of high viscosity and high ss concentration. in this study, the effectiveness of preozonation on improving the settleability of the solids in wheat and sweet potato wastewaters was investigated using a bench-scale system and pilot treatment system respectively. results showed that solid-liquid separation in the wheat wastewater was greatly improved by the decanter in the system (ss reduction from 24100 mg/l to 100 mg/l). however ... | 2004 | 15137644 |
| vasorelaxation induced by common edible tropical plant extracts in isolated rat aorta and mesenteric vascular bed. | in this study, the vasodilatory actions of nine edible tropical plant extracts were investigated. ipomoea batatas (sweet potato leaf), piper betle (betel leaf), anacardium occidentale (cashew leaf), gynandropsis gynandra (maman leaf), carica papaya (papaya leaf), and mentha arvensis (mint leaf) extracts exhibited more than 50% relaxing effect on aortic ring preparations, while piper betle and cymbopogon citratus (lemongrass stalk) showed comparable vasorelaxation on isolated perfused mesenteric ... | 2004 | 15138017 |
| feeding and survival of the malaria vector anopheles gambiae on plants growing in kenya. | the propensity of the malaria vector mosquito anopheles gambiae giles (diptera: culicidae) to ingest sugars from various plants, and subsequent survival rates, were assessed with laboratory-reared males and females offered eight species of plants commonly cultivated and/or growing wild in western kenya. in cages (no-choice bioassay), mosquitoes given the opportunity to feed on castorbean (ricinus communis l.) had the longest survival times (mean and median survival time of 6.99 +/- 0.23 and 5.67 ... | 2004 | 15189235 |
| differential expression of 10 sweetpotato peroxidase genes in response to bacterial pathogen, pectobacterium chrysanthemi. | to understand the function of each peroxidase (pod, ec 1.11.1.7) in terms of biotic stress, changes in pod specific activity and expression of 10 pod genes were investigated in four cultivars of sweetpotato (ipomoea batatas) after infection with pectobacterium chrysanthemi. pod specific activity (units mg(-1) protein) increased from 16 h after inoculation (hai) in three varieties. pod activities of two cultivars, shinwhangmi and white star, reached a maximum level at 24 hai by about three times ... | 2004 | 15191750 |
| [bio-environmental effects and index of remediation of multi-heavy metals polluted red soils]. | a pot experiment was conducted to study the bio-environmental effects of lime and organic manure application on red soil and paddy soil derived from red sandstone and polluted by multi-heavy metals. the results indicated that liming decreased the content of soil bioavailable cu and pb extracted with 0.05 mol/l hcl, while applying hog manure increased that of cd. soil bioavailable heavy metals (hms) showed an apparent relationship with soil dissolved carbon. applying lime and organic manure has a ... | 2004 | 15202245 |
| active recombinant thioredoxin h protein with antioxidant activities from sweet potato (ipomoea batatas [l.] lam tainong 57) storage roots. | recombinant thioredoxin h (trx2) overproduced in escherichia coli (m15) was purified by ni2+-chelated affinity chromatography. the molecular mass of trx2 is approximately 1.4 kda as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. total antioxidant status, 1,1-diphenyl-2-picrylhydrazyl (dpph) staining, reducing power method, fe2+-chelating ability, ferric thiocyanate (ftc) method, and protection of calf thymus dna against hydroxyl radical-induced damage were studied. the ... | 2004 | 15264905 |
| absorption of acylated anthocyanins in rats and humans after ingesting an extract of ipomoea batatas purple sweet potato tuber. | we evaluated the absorbability of anthocyanins in humans and rats administered with a beverage prepared from an extract of the tuber of purple sweet potato (ipomoea batatas cultivar ayamurasaki), or with an anthocyanin concentrate. two major anthocyanin components, cyanidin 3-o-(2-o-(6-o-(e)-caffeoyl-beta-d-glucopyranosyl)-beta-d-glucopyranoside)-5-o-beta-d-glucopyranoside) and peonidin 3-o-(2-o-(6-o-(e)-caffeoyl-beta-d-glucopyranosyl)-beta-d-glucopyranoside)-5-o-beta-d-glucopyranoside), were de ... | 2004 | 15277754 |
| efficient developmental mis-targeting by the sporamin ntpp vacuolar signal to plastids in young leaves of sugarcane and arabidopsis. | plant vacuoles are multi-functional, developmentally varied and can occupy up to 90% of plant cells. the n-terminal propeptide (ntpp) of sweet potato sporamin and the c-terminal propeptide (ctpp) of tobacco chitinase have been developed as models to target some heterologous proteins to vacuoles but so far tested on only a few plant species, vacuole types and "payload" proteins. most studies have focused on lytic and protein-storage vacuoles, which may differ substantially from the sugar-storage ... | 2004 | 15372194 |
| potential chemopreventive properties of extract from baked sweet potato (ipomoea batatas lam. cv. koganesengan). | the extract from baked sweet potato (ipomoea batatas lam. cv. koganesengan) showed potential cancer-preventing effects. the extract was partially fractionated to four fractions (i, ii-a ii-b, and iii) by sephadex g-25 gel chromatography. the cytotoxicity against human myelocytic leukemia hl-60 cells, the suppression of tpa-induced transformation in mouse skin jb6 c141 cells, the apoptosis inducing activity in hl-60 cells, and the scavenging capacity against dpph radical were tested on the four f ... | 2004 | 15537331 |
| caffeoylsophorose, a new natural alpha-glucosidase inhibitor, from red vinegar by fermented purple-fleshed sweet potato. | the suppressive effect on the postprandial blood glucose rise through alpha-glucosidase (agh) inhibition was investigated in this study in order to clarify an antihyperglycemic function of 6-o-caffeoylsophorose (cs) from diacylated anthocyanin. the administration of cs (100 mg/kg) following maltose (2 g/kg) to sprague-dawley rats resulted in the maximal blood glucose level after 30 min being significantly decreased by 11.1% compared to the control. a reduction in the serum insulin secretion was ... | 2004 | 15564660 |
| phenolic composition and radical scavenging activity of sweetpotato-derived shochu distillery by-products treated with koji. | phenolic composition and radical scavenging activity in the shochu distillery by-products of sweetpotato (ipomoea batatas l.) treated with koji (aspergillus awamori mut.) and cellulase (cellulosin t2) were investigated to develop new uses. koji and cellulosin t2 treatment of shochu distillery by-products from sweetpotatoes, rice, and barley increased phenolic content. caffeic acid was identified as a dominant phenolic component in the shochu distillery by-products of the sweetpotato. adding koji ... | 2004 | 15618617 |
| phosphate forms an unusual tripodal complex with the fe-mn center of sweet potato purple acid phosphatase. | purple acid phosphatases (paps) are a family of binuclear metalloenzymes that catalyze the hydrolysis of phosphoric acid esters and anhydrides. a pap in sweet potato has a unique, strongly antiferromagnetically coupled fe(iii)-mn(ii) center and is distinguished from other paps by its increased catalytic efficiency for a range of activated and unactivated phosphate esters, its strict requirement for mn(ii), and the presence of a mu-oxo bridge at ph 4.90. this enzyme displays maximum catalytic eff ... | 2004 | 15625111 |
| effects of combination of caiapo with other plant-derived substance on anti-diabetic efficacy in kk-ay mice. | b. ludvik et al., have recently shown the effect of caiapo (ipomoea batatas l.) on reducing fasting blood glucose and insulin resistance in type-2 diabetic patients. it, however, was required 2-4 weeks after the single administration of caiapo. the present study aimed to determine if the combination therapy of caiapo with a mulberry leaf powder, which inhibits alpha-glucosidase, or with a loquat leaf extract, which shows an insulin-like effect, could make it possible to enhance the antidiabetic ... | 2004 | 15630271 |
| feeding of spinach or sweet-potato leaves and growth performance of growing pigs kept on smallholder farms in central vietnam. | we investigated whether the addition of either spinach or sweet-potato leaves to the diet of growing pigs, kept in small holdings in central vietnam, would improve growth performance. a control diet was formulated and mixed with each of the vegetables to a final concentration of 15% of the total dietary dry matter. the diets were fed to the pigs from 70 to 100 days of age on six different smallholder farms in central vietnam. there were three animals per treatment group per farm and biopsies of ... | 2004 | 15643817 |
| advancing human nutrition without degrading land resources through modeling cropping systems in the ethiopian highlands. | food shortage in sub-saharan africa is generally considered a function of limited access to food, with little thought to nutritional quality. analyzing household production of nutrients across farming systems could be valuable in guiding the improvement of those systems. an optimization model was employed to analyze the scenario of human nutrition and cropland allocation in enset (enset ventricosum)/root crop-based and cereal-based systems of the ethiopian highlands. the type and amount of nutri ... | 2004 | 15646312 |
| effect of fermentation on sweetpotato (ipomoea batatas) toxicity in mice. | unfortunate bovine fatalities occurring after ingestion of mold-damaged sweetpotatoes preclude the use of the culled tubers in livestock feed. in cattle, mold-damaged sweetpotatoes induce an acute respiratory distress syndrome resulting in asphyxiation. because of this potential toxicity and the general abundance of culled sweetpotatoes, the detoxification efficacy of ensiling was explored since it is an easy and economically viable technique often applied to preserve livestock feed. sweetpotato ... | 2004 | 14733525 |
| further characterization of 'sweet potato virus 2': a distinct species of the genus potyvirus. | an incompletely described potyvirus isolate from sweet potato in taiwan, referred to as 'sweet potato virus 2' (spv2), was further characterised. electron microscopy revealed that spv2 has filamentous particles of 850 nm in length and induces cytoplasmic cylindrical inclusions consisting of pinwheels and scrolls. the virus was mechanically transmitted to several species of the genera chenopodium, datura, nicotiana, and ipomoea. two biotypes of myzus persicae transmitted spv2 in a non-persistent ... | 2004 | 14745592 |
| cerebroside elicitor confers resistance to fusarium disease in various plant species. | abstract in the rice blast fungus pathosystem, cerebroside, a compound categorized as a sphingolipid, was found in our previous study to be a non-racespecific elicitor, which elicits defense responses in rice. here we describe that cerebroside c is produced in diverse strains of fusarium oxysporum, a common soilborne agent of wilt disease affecting a wide range of plant species. in addition, some type of cerebroside elicitor involving cerebroside a, b, or c was detected in other soilborne phytop ... | 2004 | 18943100 |
| [alkaloids of the hairy roots of ipomoea batatas lam]. | to study the alkaloid constituents of the hairy roots of ipomoea batatas lam. | 2004 | 15700823 |
| structural characterization of the dihydroflavonol 4-reductase b (dfr-b) gene in the sweet potato. | a genomic fragment containing the dihydroflavonol 4-reductase b (dfr-b) gene was cloned from the sweet potato (ipomoea batatas) and its nucleotide sequence was analyzed. the exons and flanking regions were highly homologous to those of previously reported dfr-b genes of the japanese morning glory, whereas the introns and the intergenic region were less conserved. in addition to the sequences of three miniature inverted-repeat transposable elements (mites) and one direct repeat previously reporte ... | 2004 | 15620215 |
| characterization of acylated anthocyanins in callus induced from storage root of purple-fleshed sweet potato, ipomoea batatas l. | four anthocyanins were isolated from a highly pigmented callus induced from the storage root of purple-fleshed sweet potato (ipomoea batatas l) cultivar ayamurasaki. the anthocyanins were respectively identified as cyanidin 3- $o$ -(2- $o$ -(6- $o$ -( $e$ )-caffeoyl- $\beta$ -d-glucopyranosyl)- $\beta$ -d-glucopyranoside)-5- $o$ - $\beta$ -d-glucopyranoside, cyanidin 3- $o$ -(2- $o$ -(6- $o$ -( $e$ )- $p$ -coumaroyl- $\beta$ -d-glucopyranosyl)-6- $o$ -( $e$ )-caffeoyl- $\beta$ -d-glucopyranoside ... | 2004 | 15577190 |
| alterations in intracellular and extracellular activities of antioxidant enzymes during suspension culture of sweetpotato. | cultured plant cells are a good system for the study of antioxidant mechanisms and for the mass production of antioxidants, because they can be grown under conditions of high oxidative stress. alterations in the intracellular and extracellular activities of three antioxidant enzymes, superoxide dismutase (sod), guaiacol-type peroxidase (pod), and glutathione peroxidase (gpx), were investigated in suspension cultures of sweetpotato (ipomoea batatas) during cell growth. intracellular sod activitie ... | 2004 | 15381411 |
| birthweight and environment at tari. | the weights at birth of 4767 children born in the tari area between 1979 and 1986 were analyzed after the children had been allocated to seven environmental zones. the environments differ in the quality of land from which the staple food, sweet potato (ipomoea batatas), is produced. mean birthweight varies significantly by environment. women do much of the day-to-day agricultural work. it is argued that women living in the poorer quality environments produce less food, suffer chronic malnourishm ... | 2004 | 14658831 |
| phylogenetic relationships of the sweetpotato in ipomoea series batatas (convolvulaceae) based on nuclear beta-amylase gene sequences. | phylogenetic relationships of 13 accessions and a cultivar representing the sweetpotato, ipomoea batatas (l.) lam., and its wild progenitors, were investigated using the nucleotide sequence variation of a nuclear-encoded beta-amylase gene. a 1.1-1.3 kb fragment of the gene spanning two exons separated by a long intron was pcr-amplified, cloned, and sequenced. exon sequences proved highly conservative, while intron sequences yielded large differences. intron analyses grouped species in a phylogen ... | 2004 | 15012943 |
| efficacy of ipomoea batatas (caiapo) on diabetes control in type 2 diabetic subjects treated with diet. | to investigate the tolerability, efficacy, and mode of action of caiapo, an extract of white sweet potatoes, on metabolic control in type 2 diabetic patients. | 2004 | 14747225 |
| daily consumption of indian spinach (basella alba) or sweet potatoes has a positive effect on total-body vitamin a stores in bangladeshi men. | recent evidence suggests that the vitamin a equivalency of beta-carotene from plant sources is lower than previously estimated. | 2004 | 15321812 |
| inactivation of trypsin inhibitors in sweet potato and taro tubers during processing. | in order to understand the extent of elimination of trypsin inhibitors during processing of sweet potato (ipomoea batatas) and taro (colocasia esculenta) tubers, a detailed study was conducted using tubers processed by oven drying, cooking, and microwave baking. between 80 and 90% trypsin inhibitor (ti) activity was retained in sweet potato chips up to 2h at 70 degrees c. among the four cultivars of sweet potatoes, rs-iii-2 trypsin inhibitors were more heat labile. heating at 100 degrees c led t ... | 2003 | 12906353 |
| tuber storage proteins. | a wide range of plants are grown for their edible tubers, but five species together account for almost 90 % of the total world production. these are potato (solanum tuberosum), cassava (manihot esculenta), sweet potato (ipomoea batatus), yams (dioscorea spp.) and taro (colocasia, cyrtosperma and xanthosoma spp.). all of these, except cassava, contain groups of storage proteins, but these differ in the biological properties and evolutionary relationships. thus, patatin from potato exhibits activi ... | 2003 | 12730067 |
| ion chromatography of phytate in roots and tubers. | the ion chromatographic method for the quantification of phytate (insp(6)) in foods was adapted for the analysis of roots and tubers. to maximize sensitivity, ultraviolet (uv) detection following postcolumn derivatization was compared with evaporative light-scattering detection (elsd). detection limits for phytate were 0.5 and 1 microg for uv and elsd, respectively. unidentified peaks eluting close to and after insp(6) were removed by solid-phase extraction. phytate was detected in 11 of 15 root ... | 2003 | 12517094 |
| invertase inhibitors from sweet potato (ipomoea batatas): purification and biochemical characterization. | two proteinaceous invertase inhibitors, designated iti-l and iti-r, were purified to electrophoretic homogeneity. iti-l was purified from acetone powder of sweet potato leaves through sequential steps entailing buffer extraction, acid treatment, deae-sephacel ion-exchange chromatography, and sephacryl s-100 gel filtration. iti-r was purified from sweet potato tuberous roots by sequentially applying buffer extraction, con a-sepharose affinity chromatography, deae-sephacel ion-exchange chromatogra ... | 2003 | 14705916 |
| recycling plant wax constituents for chemical defense: hemi-biosynthesis of triterpene saponins from beta-amyrin in a leaf beetle. | several species of doryphorina leaf beetles from central- and south america produce oleanane triterpene glycosides in their defensive glands. the presence of pentacyclic triterpenes in insects is intriguing since they lack the key enzymes necessary to synthesize these compounds. since beta-amyrin is a common constituent of leaf waxes, we hypothesized that these leaf beetles use this compound as a precursor to their oleanane glycosides. to test this hypothesis we first confirmed the presence of b ... | 2003 | 14610652 |
| mode of action of ipomoea batatas (caiapo) in type 2 diabetic patients. | we have previously reported the beneficial effects of caiapo, the extract of white-skinned sweet potato (ipomoea batatas), on fasting plasma glucose, as well as on total and low-density lipoprotein (ldl) cholesterol in type 2 diabetic patients. the present study aimed to describe the underlying mechanism responsible for the improvement in metabolic control following administration of caiapo in those type 2 subjects. a total of 18 male patients (age=58+/-8 years, body mass index [bmi]=27.7+/-2.7 ... | 2003 | 12870164 |
| simplified cryopreservation of sweet potato [ ipomoea batatas (l.) lam.] by optimizing conditions for osmoprotection. | shoot tips of sweet potato were successfully cryopreserved using an encapsulation vitrification method. encapsulated shoot tips were pre-incubated in liquid murashige-skoog medium containing 30 g/l sucrose for 24 h, then precultured in sucrose-enriched medium (0.3 m sucrose) for 16 h. shoot tips were osmoprotected with a mixture of 2 m glycerol and 1.6 m sucrose for 3 h before being dehydrated with a highly concentrated vitrification solution (pvs2) for 1 h at 25 degrees c. the encapsulated and ... | 2003 | 12835905 |
| gene expression activity and pathway selection for sucrose metabolism in developing storage root of sweet potato. | development of sweet potato (ipomoea batatas) storage root coincides with starch accumulation made using cleaved products of imported photoassimilate sucrose. the genes and pathways are predominantly active for sucrose metabolism in developing storage root were unknown. in this study, we used both an expressed sequence tag (est) approach and a reverse transcription-polymerase chain reaction (rt-pcr) approach to answer this question. sucrose synthase (susy) was found to be significantly more freq ... | 2003 | 12826628 |
| molecular cloning of two metallothionein-like protein genes with differential expression patterns from sweet potato (ipomoea batatas) leaves. | metallothionein (mt) is a group of proteins with low molecular masses and high cysteine contents, and is classified into different types, which in general contains two domains (domain 1 and domain 2) with typical amino acid sequences (rauser 1999). in this report two full-length cdnas (y459 and g14) encoding mt-like proteins were isolated from leaves of sweet potato (ipomoea batatas). their open reading frames contained 249 and 195 nucleotides (82 and 64 amino acids) for y459 and g14, respective ... | 2003 | 12806784 |
| differential expression of six novel peroxidase cdnas from cell cultures of sweetpotato in response to stress. | six peroxidase (pod) cdnas were isolated from suspension cultures of sweetpotato (ipomoea batatas) by cdna library screening, and their expression was investigated with a view to understanding the physiological functions of each pod in relation to environmental stress. the gene products encoded by these cdnas could be divided into two groups, anionic pods (swpa4, swpa5, swpa6) and basic pods (swpb1, swpb2, swpb3), on the basis of the predicted pi values of the mature proteins. rt-pcr analysis re ... | 2003 | 12802681 |
| involvement of hydrogen peroxide and nitric oxide in expression of the ipomoelin gene from sweet potato. | the ipo (ipomoelin) gene was isolated from sweet potato (ipomoea batatas cv tainung 57) and used as a molecular probe to investigate its regulation by hydrogen peroxide (h(2)o(2)) and nitric oxide (no) after sweet potato was wounded. the expression of the ipo gene was stimulated by h(2)o(2) whether or not the plant was wounded, but its expression after wounding was totally suppressed by the presence of diphenylene iodonium, an inhibitor of nadph oxidase, both in the local and systemic leaves of ... | 2003 | 12746543 |
| stoichiometric and kinetic studies of phenolic antioxidants from andean purple corn and red-fleshed sweetpotato. | stoichiometric and kinetic values of phenolics against dpph (2,2-diphenyl-1-picrylhydrazyl) were determined for andean purple corn (zea mays l.) and red sweetpotato (ipomoea batatas). both crops had higher antioxidant capacity and antiradical kinetics than blueberries and higher or similar anthocyanin and phenolic contents. the second-order rate constant (k(2)) was 1.56, 1.12, 0.57, and 0.26 (mg antiradical/ml)(-1) s(-1) for red sweetpotato, trolox, purple corn, and blueberry, respectively. on t ... | 2003 | 12744660 |
| quantity and potential biological activity of caffeic acid in sweet potato [ipomoea batatas (l.) lam.] storage root periderm. | the caffeic acid content of storage root periderm and cortex tissues of genetically diverse sweet potato [ipomoea batatas (l.) lam.] cultivars and breeding clones was quantified by high-performance liquid chromatography. periderm caffeic acid content of the clones ranged from 0.008 to 7.97 mg/g dry weight, whereas the highest cortex content was 0.047 mg/g. clones varied greatly in periderm caffeic acid content in all experiments, but there were also differences between experiments in content ave ... | 2003 | 12720375 |
| caffeoylsophorose in a red vinegar produced through fermentation with purple sweetpotato. | recently, a new red vinegar has been developed via fermentation with the storage root of purple-fleshed sweetpotato, ipomoea batatas l. cv. ayamurasaki. the red vinegar had a higher antioxidative activity than white or black vinegars. the red vinegar contained some new components possibly derived from the original purple sweetpotato. a major component was isolated using preparative hplc, and the chemical structure was determined to be 6-o-(e)-caffeoyl-(2-o-beta-d-glucopyranosyl)-alpha-d-glucopyr ... | 2003 | 12696933 |
| genetic variability and host specialization in the latin american clade of ceratocystis fimbriata. | abstract the ceratocystis fimbriata complex includes many undescribed species that cause wilt and canker diseases of many economically important plants. phylogenetic analyses of dna sequences have delineated three geographic clades within ceratocystis fimbriata. this study examined host specialization in the latin american clade, in which a number of lineages were identified using sequences of the internal transcribed spacer (its) region of the rdna. three host-associated lineages were identifie ... | 2003 | 18944327 |
| sensory characterization of a ready-to-eat sweetpotato breakfast cereal by descriptive analysis. | the sweetpotato [ipomoea batatas (l.) lam], an important industry in the united states, has been selected as a candidate crop to be grown on future long-duration space missions by nasa. raw sweetpotato roots were processed into flour, which was used to formulate ready-to-eat breakfast cereal (rtebc). twelve trained panelists evaluated the sensory attributes of the extruded rtebc using descriptive analysis. the samples were significantly different (p<0.05) for all attributes. twelve perceived sen ... | 2003 | 14974490 |
| applying a nutribusiness approach to increase animal source food consumption in local communities. | animal source foods (asf) in the diets of schoolchildren are beneficial for supporting optimal physical and cognitive development. nevertheless, behavioral change and economic development are needed to increase and sustain adequate meat product consumption by schoolchildren in developing countries. a nutribusiness enterprise may be one way for local communities to promote economic development while increasing the availability of meat for children. this work evaluates the feasibility of a nutribu ... | 2003 | 14672307 |
| expression and characterization of sweet potato invertase in pichia pastoris. | an invertase cdna (ibbetafruct1) was cloned from sweet potato leaves and characterized. the deduced amino acid sequence of the ibbetafruct1-encoded protein was closely related to vacuolar invertases and included the wecvd catalytic domain characteristic of them. an expression plasmid containing the coding region of ibbetafruct1 under the control of the alcohol oxidase promoter was used to transform the methylotrophic yeast pichia pastoris. the biochemical properties for the expressed recombinant ... | 2003 | 12590504 |
| sequence variability within the 3'-proximal part of the sweet potato mild mottle virus genome. | sweet potato mild mottle virus (spmmv) is the type member of the genus ipomovirus (family potyviridae) and is only known to occur in east africa. in uganda, spmmv is the third most prevalent virus infecting sweet potato. the sequence variability of spmmv was studied by cloning and sequencing a 1.8-kb fragment representing the 3'-end of the genome of eight spmmv isolates collected from different districts of uganda. sequence comparisons indicated 85.9-99.9% nucleotide sequence identity and 92.8-1 ... | 2003 | 12607100 |
| potential functional foods in the traditional maori diet. | the maori people were early new zealand settlers of polynesian descent. the incidence of non-infectious diseases appears to have been low in these people, perhaps in part due to the presence of protective chemical constituents within their food plant supply. three of the tropical crops they introduced are still eaten here today: the sweet potato or kumara (ipomoea batatas), the taro (colocasia esculenta) and the cabbage tree or ti (cordyline terminalis). sporamins a and b, the major storage prot ... | 2003 | 12628508 |
| candida kunwiensis sp. nov., a yeast associated with flowers and bumblebees. | a novel asexual ascomycetous yeast, candida kunwiensis (sg99-26t = kctc 17041t = cbs 9067t), was isolated from sweet potato (ipomoea batatas) flowers in korea and from the body surface of pollinating bumblebees in germany. comparative analysis of the d1/d2 domain of 26s rdna of all available sequences for ascomycetous yeasts showed that the novel species was phylogenetically related to the genus metschnikowia, but the sequence similarity was low. morphologically and physiologically, c. kunwiensi ... | 2003 | 12656196 |
| molecular biology and application of plant peroxidase genes. | peroxidases are a family of isozymes found in all plants; they are heme-containing monomeric glycoproteins that utilize either h(2)o(2) or o(2) to oxidize a wide variety of molecules. these important enzymes are used in enzyme immunoassays, diagnostic assays and industrial enzymatic reactions. peroxidase genes and their promoters can be used for molecular breeding of useful plants. transgenic techniques have also been used to investigate the physiological and molecular functions of peroxidase ge ... | 2003 | 12664144 |
| an elisa-based method for measurement of food-specific ige antibody in mouse serum: an alternative to the passive cutaneous anaphylaxis assay. | passive cutaneous anaphylaxis (pca) assay has been a gold standard method to measure allergen-specific ige antibody (asige ab) levels in allergy mouse models. many factors including stringent guidelines for laboratory animal use make pca a difficult choice. therefore, alternative methods are needed that can be readily applied for measurement of specific ige antibody levels in mouse serum. herein we describe a novel elisa-based method that is more sensitive in comparison to pca, ige isotype-speci ... | 2003 | 12667673 |
| food protein-induced enterocolitis syndrome caused by solid food proteins. | infantile food protein-induced enterocolitis syndrome (fpies) is a severe, cell-mediated gastrointestinal food hypersensitivity typically provoked by cow's milk or soy. solid foods are rarely considered a cause. | 2003 | 12671120 |
| interstrain inhibition in the sweet potato pathogen streptomyces ipomoeae: purification and characterization of a highly specific bacteriocin and cloning of its structural gene. | strains of the sweet potato soil rot pathogen streptomyces ipomoeae had previously been divided into three groups based on their ability to inhibit one another during pairwise cocultivation. while group i strains are not antagonistic to members of the other groups, group ii and group iii strains produce separate substances that are inhibitory to strains outside their respective cognate groups. here, we purified the group iii inhibitory substance from the culture supernatant of a representative s ... | 2003 | 12676701 |
| a novel oxidative stress-inducible peroxidase promoter from sweetpotato: molecular cloning and characterization in transgenic tobacco plants and cultured cells. | a strong oxidative stress-inducible peroxidase (pod) promoter was cloned from sweetpotato (ipomoea batatas) and characterized in transgenic tobacco plants and cultured cells in terms of environmental stress. a pod genomic clone (referred to as swpa2) consisted of 1824 bp of sequence upstream of the translation start site, two introns (743 bp and 97 bp), and a 1073 bp coding region. swpa2 had previously been found to encode an anionic pod which was highly expressed in response to oxidative stress ... | 2003 | 12777043 |
| sporamin-mediated resistance to beet cyst nematodes (heterodera schachtii schm.) is dependent on trypsin inhibitory activity in sugar beet (beta vulgaris l.) hairy roots. | sporamin, a sweet potato tuberous storage protein, is a kunitz-type trypsin inhibitor. its capability of conferring insect-resistance on transgenic tobacco and cauliflower has been confirmed. to test its potential as an anti-feedant for the beet cyst nematode (heterodera schachtii schm.), the sporamin gene spti-1 was introduced into sugar beet (beta vulgaris l.) by agrobacterium rhizogenes-mediated transformation. twelve different hairy root clones expressing sporamin were selected for studying ... | 2003 | 12777044 |
| expression and characterization of an anti-(hepatitis b surface antigen) glycosylated mouse antibody in transgenic tobacco (nicotiana tabacum) plants and its use in the immunopurification of its target antigen. | transgenic plants expressing recombinant immunoglobulins have arisen as an alternative technology for the large-scale production of antibodies useful in therapeutics and in industrial processes. in the present paper we report the expression in transgenic tobacco ( nicotiana tabacum ) of an anti-hbsag [anti-(hepatitis b virus surface antigen)] mouse igg1 mab (monoclonal antibody), currently used for the industrial purification of the recombinant vaccine antigen. using the sweet potato sporamin si ... | 2003 | 12797866 |
| identification of genes in the tomato big bud phytoplasma and comparison to those in sweet potato little leaf-v4 phytoplasma. | genetic relatedness of phytoplasmas is commonly defined on the basis of differences in the highly conserved 16s rrna gene, which may not resolve closely related phytoplasmas. an example of this is the closely related tomato big bud (tbb) and sweet potato little leaf strain v4 (spll-v4) phytoplasmas, which cannot easily be differentiated by their 16s rrna gene sequences. this study aimed to identify genes on the tbb phytoplasma chromosome which could be used to examine genetic variation between t ... | 2003 | 12855731 |
| development and transfer of genetically modified virus-resistant sweet potato for subsistence farmers in kenya. | up to 80% of the yield of sweet potato, the major root crop in east africa, can be destroyed by crop diseases such as the sweet potato feathery mottle virus (spfmv). a sweet potato that is resistant to spfmv is being developed through the combined efforts of non-governmental agenices, biotechnology groups, and agricultural entities. even small improvements in crop yield, owing to this new technology, will feed countless people in africa. | 2003 | 12908741 |
| production of galactinol from sucrose by plant enzymes. | galactinol, 1-o-(alpha-d-galactopyranosyl)-myo-inositol, was produced from sucrose as a starting material. udp-glc was prepared with sucrose and udp using sucrose synthase partially purified from sweet potato roots. then, the udp-glc was converted to udp-gal using yeast udp-gal 4-epimerase from a commercial source. finally, galactinol was produced from the udp-gal and myo-inositol using galactinol synthase partially purified from cucumber leaves. the product was identified as galactinol by the r ... | 2003 | 12913288 |
| coat protein sequence analysis reveals occurrence of new strains of sweet potato feathery mottle virus in uganda and tanzania. | the 3'-proximal part (1.8 kb) of the sweet potato feathery mottle virus (spfmv) genome was studied in four spfmv isolates collected from farmers' fields in western uganda (spfmv-bny), eastern uganda (spfmv-sor) and bagamoyo district, tanzania (spfmv-tz1 and spfmv-tz2). unlike the other three spfmv isolates, spfmv-sor was not detected with the polyclonal antisera to spfmv. it showed moderately high coat protein (cp) nucleotide (93.3-96.7%) and amino acid (93.6-96.8%) sequence identity to the isol ... | 2003 | 12913357 |
| further variability within the genus crinivirus, as revealed by determination of the complete rna genome sequence of cucurbit yellow stunting disorder virus. | the complete nucleotide (nt) sequences of genomic rnas 1 and 2 of cucurbit yellow stunting disorder virus (cysdv) were determined for the spanish isolate cysdv-allm. rna1 is 9123 nt long and contains at least five open reading frames (orfs). computer-assisted analyses identified papain-like protease, methyltransferase, rna helicase and rna-dependent rna polymerase domains in the first two orfs of rna1. this is the first study on the sequences of rna1 from cysdv. rna2 is 7976 nt long and contains ... | 2003 | 12917477 |
| potential chemopreventive properties of anthocyanin-rich aqueous extracts from in vitro produced tissue of sweetpotato (ipomoea batatas l.). | anthocyanin-rich aqueous extracts from cell suspension cultures of a high anthocyanin-producing sweetpotato pl (purple line) cell line grown under two different media conditions, mm (multiplication medium) and apm (high anthocyanin-producing medium) and from the cell line's donor tissue, field-grown storage root (sr) of sweetpotato, cv. ayamurasaki, were evaluated for antioxidative (dpph test), antimutagenic (salmonella/reversion assay; mutagen, trp-p-1), and antiproliferative (human promyelocyt ... | 2003 | 13129295 |
| [study of ceratocystis fimbriata toxins determination from sweet potato by high performance thin-layer chromatography]. | the extract solution from sweet potato was washed with 5% na2co3 and followed with distilled water and dried with dehydrated sodium sulfate (na2so4). the ether extract was evaporated and the residue (crude sample) was dissolved in a small volume of chloroform-soluble solution and was spotted on a silica gel g thin-layer plate developed with different solvent systems. pure ipomeamarone and ipomeamaronol were used as standards. the resolution was the best in the solvent system of petroleum ether-e ... | 2003 | 14535108 |
| the ai project: historical and ecological contexts. | this paper aims to review a long-term research project exploring the chimpanzee mind within historical and ecological contexts. the ai project began in 1978 and was directly inspired by preceding ape-language studies conducted in western countries. however, in contrast with the latter, it has focused on the perceptual and cognitive capabilities of chimpanzees rather than communicative skills between humans and chimpanzees. in the original setting, a single chimpanzee faced a computer-controlled ... | 2003 | 14566577 |
| endophytic nifh gene diversity in african sweet potato. | a cultivation-independent approach was used to identify potentially nitrogen-fixing endophytes in seven sweet potato varieties collected in uganda and kenya. nitrogenase reductase genes (nifh) were amplified by pcr, and amplicons were cloned in escherichia coli. clones were grouped by restriction fragment length polymorphism analysis, and representative nifh genes were sequenced. the resulting sequences had high homologies to nitrogenase reductases from alpha-, beta-, and gamma-proteobacteria an ... | 2003 | 14608421 |
| antioxidant and memory enhancing effects of purple sweet potato anthocyanin and cordyceps mushroom extract. | the effects of purple sweet potato anthocyanin (spa) and cordyceps mushroom extract (cme) on lipid peroxidation, 1,1-diphenyl-2-picrylhydrazyl (dpph) radicals and cognitive deficits were examined. both spa and cme exhibited dpph radical scavenging activities with similar potency. in contrast, only spa was shown to effectively inhibit lipid peroxidation initiated by fe2+ and ascorbic acid in rat brain homogenates. furthermore, spa markedly enhanced cognitive performance, assessed by passive avoid ... | 2003 | 14609130 |
| change in the crystal packing of soybean beta-amylase mutants substituted at a few surface amino acid residues. | in spite of the high similarity of amino acid sequence and three-dimensional structure between soybean beta-amylase (sba) and sweet potato beta-amylase (spb), their quaternary structure is quite different, being a monomer in sba and a tetramer in spb. because most of the differences in amino acid sequences are found in the surface region, we tested the tetramerization of sba by examining mutations of residues located at the surface. we designed the sba tetramer using the spb tetramer structure a ... | 2003 | 14631070 |
| development of a pilot system for converting sweet potato starch into glucose syrup. | sweet potato has been chosen as one of nasa's crops to support human beings in future space missions. one of the possible uses is to make syrup that can be used as a general sweetener. in this work a simple engineering system for converting sweet potato starch into glucose syrup was studied on a laboratory scale. the system comprises the following main units: a blender, continuous stirred tank reactor (cstr), centrifugal and vacuum filters, deionization column and vacuum evaporator. the system w ... | 2003 | 14631997 |
| some molecular characteristics of three viruses from spvd-affected sweet potato plants in egypt. | sweet potato feathery mottle virus (spfmv, genus potyvirus, family potyviridae), sweet potato chlorotic stunt virus (spcsv, genus crinivirus, family closteroviridae) and sweet potato virus g (spvg) were detected in naturally infected sweet potato plants grown in the delta region in egypt. before this study, spvg was reported only from china. two isolates of spfmv and one isolate of spvg were characterized for the 3'-proximal genomic sequences. phylogenetic analyses indicated that the spfmv isola ... | 2003 | 14648298 |
| a new method for the preparation of beta-amylase from sweet potato. | a simple method for the preparation of sweet potato beta-amylase by thymol amylose adsorption is described. the method is far more efficient and gives higher recovery of the enzyme. the crystalline enzyme thus obtained is found to be homogeneous by gel chromatography, polyacrylamide gel electrophoresis. | 2003 | 155814 |
| cyclometalated ruthenium(ii) complexes as efficient redox mediators in peroxidase catalysis. | cyclometalated ruthenium(ii) complexes, [ru(ii)(c~n)(n~n)(2)]pf(6) [hc~n=2-phenylpyridine (hphpy) or 2-(4'-tolyl)pyridine; n~n=2,2'-bipyridine, 1,10-phenanthroline, or 4,4'-dimethyl-2,2'-bipyridine], are rapidly oxidized by h(2)o(2) catalyzed by plant peroxidases to the corresponding ru(iii) species. the commercial isoenzyme c of horseradish peroxidase (hrp-c) and two recently purified peroxidases from sweet potato (spp) and royal palm tree (rptp) have been used. the most favorable conditions fo ... | 2003 | 12774217 |
| bienzyme biosensors for glucose, ethanol and putrescine built on oxidase and sweet potato peroxidase. | amperometric biosensors for glucose, ethanol, and biogenic amines (putrescine) were constructed using oxidase/peroxidase bienzyme systems. the h(2)o(2) produced by the oxidase in reaction with its substrate is converted into a measurable signal via a novel peroxidase purified from sweet potato peels. all developed biosensors are based on redox hydrogels formed of oxidases (glucose oxidase, alcohol oxidase, or amine oxidase) and the newly purified sweet potato peroxidase (spp) cross-linked to a r ... | 2003 | 12706582 |
| the ecology of huli subsistence agriculture. | a detailed study of the ecology of subsistence agriculture in the tari basin was conducted to investigate the stability of intensive agroecosystems in the highlands of papua new guinea. the highly intensive agricultural systems that have evolved in the wetland environments in the lowest parts of the basin were found to be extremely stable and capable of further intensification. both soil fertility and sweet potato yields showed no signs of degradation even though some areas had been under contin ... | 2002 | 14658826 |
| wound-response regulation of the sweet potato sporamin gene promoter region. | sporamin, a tuberous storage protein of sweet potato, was systemically expressed in leaves and stems by wound stimulation. in an effort to demonstrate the regulatory mechanism of wound response on the sporamin gene, a 1.25 kb sporamin promoter was isolated for studying the wound-induced signal transduction. two wound response-like elements, a g box-like element and a gcc core-like sequence were found in this promoter. a construct containing the sporamin promoter fused to a beta-glucuronidase (gu ... | 2002 | 11855724 |
| direct absorption of acylated anthocyanin in purple-fleshed sweet potato into rats. | absorption of acylated anthocyanins in purple-fleshed sweet potato (ipomoea batatas cv. ayamurasaki) in rats was studied to obtain evidence that the acylated anthocyanins themselves could exert a physiological function in vivo. peonidin 3-caffeoylsophoroside-5-glucoside (pn 3-caf*sop-5-glc) in purple-fleshed sweet potato was directly absorbed into rat and present as an intact acylated form in plasma. after oral administration of the purple-fleshed sweet potato anthocyanin (psa) concentrate conta ... | 2002 | 11879056 |
| prevention by natural food anthocyanins, purple sweet potato color and red cabbage color, of 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (phip)-associated colorectal carcinogenesis in rats initiated with 1,2-dimethylhydrazine. | the potential of purple sweet potato color (pspc) and red cabbage color (rcc), natural anthocyanin food colors, to modify colorectal carcinogenesis was investigated in male f344/ducrj rats, initially treated with 1,2-dimethylhydrazine (dmh) and receiving 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (phip) in the diet. after dmh initiation, pspc and rcc were given at a dietary level of 5.0% in combination with 0.02% phip until week 36. no pspc or rcc-treatment-related changes in clinical signs ... | 2002 | 11915369 |
| magnetite-alginate beads for purification of some starch degrading enzymes. | starch degrading enzymes, viz., beta-amylase, glucoamylase, and pullulanase, were purified using magnetite-alginate beads. in each case, the enzyme activity was eluted by using 1.0 m maltose. beta-amylase (sweet potato), glucoamylase (aspergillus niger), and pullulanase (bacillus acidopullulyticus) from their crude preparations were purified 37-, 31-, and 49-fold with 86, 87, and 95% activity recovery, respectively. sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis showed single ... | 2002 | 11936253 |
| spectral shift of ultraweak photon emission from sweet potato during a defense response. | consecutive spectral analyses of ultraweak photon emission from sweet potato showing a defense response were conducted to observe the process of physiological transition. the spectrum showed a drastic transition from 2 to 10 h after inoculation with fusarium oxysporum, during which the emission intensity increased slowly. the spectrum was stable from 10 to 36 h after inoculation, whereas the emission intensity peaked approximately 20 h after inoculation. a change in the physiological state conne ... | 2002 | 11950100 |
| in vitro fermentation properties of selected fructooligosaccharide-containing vegetables and in vivo colonic microbial populations are affected by the diets of healthy human infants. | salsify (tragopogon porrifolius) and gobo (arctium lappa) are root vegetables that contain high concentrations of naturally occurring fructooligosaccharides (fos), nondigestible oligosaccharides that have prebiotic effects on the intestinal microflora. the objective of this study was to compare colonic microbial populations and the fermentation characteristics of gobo and salsify in breast-fed vs. formula-fed infants. fecal inoculum from breast-fed and formula-fed infants consuming either no sol ... | 2002 | 12163660 |
| complete genome sequence and analyses of the subgenomic rnas of sweet potato chlorotic stunt virus reveal several new features for the genus crinivirus. | the complete nucleotide sequences of genomic rna1 (9,407 nucleotides [nt]) and rna2 (8,223 nt) of sweet potato chlorotic stunt virus (spcsv; genus crinivirus, family closteroviridae) were determined, revealing that spcsv possesses the second largest identified positive-strand single-stranded rna genome among plant viruses after citrus tristeza virus. rna1 contains two overlapping open reading frames (orfs) that encode the replication module, consisting of the putative papain-like cysteine protei ... | 2002 | 12186910 |
| synthesis, structure, properties, and phosphatase-like activity of the first heterodinuclear fe(iii)mn(ii) complex with the unsymmetric ligand h(2)bpbpmp as a model for the pap in sweet potato. | the new heterodinuclear mixed valence complex [fe(iii)mn(ii)(bpbpmp)(oac)(2)]clo(4) (1) with the unsymmetrical n(5)o(2) donor ligand 2-bis[((2-pyridylmethyl)-aminomethyl)-6-((2-hydroxybenzyl)(2-pyridylmethyl))-aminomethyl]-4-methylphenol (h(2)bpbpmp) has been synthesized and characterized. compound 1 crystallizes in the monoclinic system, space group p2(1)/c, and has an fe(iii)mn(ii)(mu-phenoxo)-bis(mu-carboxylato) core. two quasireversible electron transfers at -870 and +440 mv versus fc/fc(+) ... | 2002 | 12206683 |
| after the 'affluent society': cost of living in the papua new guinea highlands according to time and energy expenditure-income. | what is the cost of living in the papua new guinea highlands? an answer is sought using a time and energy accounting approach. the subsistence regime of wola-speaking highlanders, the subjects of this investigation, comprises three components. the principal one is horticulture: people clearing gardens from forest and grassland, with tuberous crops predominating, notably sweet potato. the second component comprises animal rearing, notably of domestic pigs. the third, and least important, is hunti ... | 2002 | 12395862 |
| bioconversion of acid- and gamma-ray-treated sweet potato residue to microbial protein by mixed cultures. | sweet potato residue, a starchy agricultural waste, was used as a substrate to produce microbial protein by fusarium moniliforme and saccharomyces cerevisiae in submerged fermentation. acid- and gamma-irradiation-pretreated sweet potato residue enhanced the biomass yield and protein production when the residue was fermented with f. moniliforme and s. cerevisiae. a mixed culture of f. moniliforme and s. cerevisiae efficiently and rapidly utilized free sugars; the maximal biomass yield (13.96 g/l) ... | 2002 | 12407461 |
| anti-hyperglycemic effect of diacylated anthocyanin derived from ipomoea batatas cultivar ayamurasaki can be achieved through the alpha-glucosidase inhibitory action. | to clarify a postprandial glucose suppression effect of diacylated anthocyanin with alpha-glucosidase (agh) inhibitory activity, a single oral administration study of it in male 8-week-old sprague-dawley rats was performed. the diacylated anthocyanin used in this study was peonidin 3-o-[2-o-(6-o-e-feruloyl-beta-d-glucopyranosyl)-6-o-e-caffeoyl-beta-d-glucopyranoside]-5-o-beta-d-glucopyranoside isolated from storage roots of the purple sweet potato (ipomoea batatas cv. ayamurasaki), which showed ... | 2002 | 12452639 |
| antimutagenicity of mono-, di-, and tricaffeoylquinic acid derivatives isolated from sweetpotato (ipomoea batatas l.) leaf. | the caffeoylquinic acid derivatives, 3-mono-o-caffeoylquinic acid (chlorogenic acid, cha), 3,4-di-o-caffeoylquinic acid (3,4-dicqa), 3,5-di-o-caffeoylquinic acid (3,5-dicqa), 4,5-di-o-caffeoylquinic acid (4,5-dicqa) and 3,4,5-tri-o-caffeoylquinic acid (3,4,5-tricqa), and caffeic acid (ca) were isolated from the sweetpotato (ipomoea batatas l.) leaf. we examined the antimutagenicity of these caffeoylquinic acid compounds to promote new uses of the sweetpotato leaf. these caffeoylquinic acid deriv ... | 2002 | 12506969 |
| monoxenic in vitro production and colonization potential of am fungus glomus intraradices. | the paper reports the establishment of mycorrhizal infection of a non-mycorrhizal ri-t-dna transformed carrot root when co-cultured with a surface sterilized sweet potato root segment colonized by arbuscular mycorrhizal (am) fungus g. intraradices on minimal m medium. extensive fungal hyphal emergence from each cut end of the mycorrhizal sweet potato root piece was observed in one week old cultures. these hyphae caused infection on contacting the transformed-carrot- root segment and produced man ... | 2002 | 12587745 |
| [effects of nacl stress on seedling growth and iaa metabolism of sweet potato and its relation to salt-tolerance]. | seedlings of three sweet-potato (ipomoea batatas) cultivars with different salt-tolerance were stressed with different concentrations of nacl during the seedling stage. plant growth, leaf area growth, and iaa content decreased with increasing nacl concentration, while the activities of pod and iaa oxidase increased. the extent of plant growth, leaf area growth and iaa content decrease and the extent of pod and iaa oxidase activity increase were larger in sensitive cultivar than those in moderate ... | 2002 | 12557681 |
| anthocyanin compositions in sweetpotato (ipomoea batatas l.) leaves. | the anthocyanin composition of three varieties, simon no. 1, kyushu no. 119, and elegant summer, in sweetpotato (ipomoea batatas l.) leaves was examined for promoting new uses. fifteen anthocyanin compounds were identified and measured. hplc clearly showed quantitative differences, but not qualitative ones. the anthocyanins were acylated cyanidin and peonidin type. the result suggests that the major anthocyanin composition of sweetpotato leaves is cyanidin type. | 2002 | 12506993 |
| identification and characterization of foliar polyphenolic composition in sweetpotato (ipomoea batatas l.) genotypes. | trials over two years were conducted using 1389 sweetpotato (ipomoea batatas l.) genotypes collected from all over the world to characterize the polyphenolic composition in sweetpotato leaves. wide variation was observed in relation to their total and individual leaf polyphenolic constituents. in all genotypes studied, the total polyphenol contents of sweetpotato leaf ranged from 1.42 to 17.1 g/100 g dry weight. the six different polyphenolic compounds were identified and quantified by nmr, fabm ... | 2002 | 12059149 |
| comparative modeling of the latent form of a plant catechol oxidase using a molluskan hemocyanin structure. | the structure of the precursor form of catechol oxidase from sweet potatoes (ipomoea batatas) has been modeled on the basis of the 3d structural data of mature catechol oxidase [nat. struct. biol. 5 (1998) 1084] and of hemocyanin from giant octopus (octopus dofleini) [j. mol. biol. 278 (1998) 855]. a c-terminal extension peptide is found in the cdna sequence but not in the purified, mature form of catechol oxidase. superimposition of the 3d structures of the native hemocyanin and catechol oxidas ... | 2002 | 11931976 |
| isolation of mads-box genes from sweet potato (ipomoea batatas (l.) lam.) expressed specifically in vegetative tissues. | new mads-domain genes, ibmads3 and ibmads4, were isolated from pigmented and tuber-forming root tissue in sweet potato (ipomoea batatas l.). both genes were expressed preferentially in vegetative tissues, especially root tissues; white fibrous roots, pigmented roots, and developing tuberous roots. on sequence alignment, these genes fell into the stmads group composed of svp, stmads11, stmads16 and agl24, which share high sequence similarity, similar expression patterns and similar function. tran ... | 2002 | 11917086 |