Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| frontiers of neurology: a symposium in honor of fred plum. new york city, new york, usa. october 5, 1996. | 1997 | 9634449 | |
| susceptibility of peach gf 305 seedlings and selected herbaceous plants to plum pox virus isolates from western slovakia. | the susceptibility of peach gf 305 seedlings and herbaceous plants to five plum pox virus (ppv) isolates from orchards of western slovakia was investigated. ppv was isolated from diseased plum, apricot and peach trees, and transmitted by chip-budding to peach gf 305. the herbaceous plants were infected by mechanical inoculation. the transmission was analysed by symptomatology and double sandwich enzyme-linked immunosorbent assay (das-elisa). infected peaches developed leaf distortion, tissue cle ... | 1997 | 9607094 |
| production of strain specific antibodies against a synthetic polypeptide corresponding to the n-terminal region of the plum pox potyvirus coat protein. | comparison of the predicted coat protein amino acid sequence of the 'sweet cherry' strain of plum pox potyvirus (ppv-swc) with the corresponding regions of several other ppv strains indicated that the main differences are in the n-terminal region. polyclonal antibodies were produced against a synthetic peptide corresponding to the 1-14 sequence of the n-terminal region of ppv-swc coat protein. they specifically detected ppv-swc in different immunochemical tests. | 1997 | 9504763 |
| analysis of the sequence diversity of the p1, hc, p3, nib and cp genomic regions of several yam mosaic potyvirus isolates: implications for the intraspecies molecular diversity of potyviruses. | partial sequences from serologically characterized yam mosaic potyvirus (ymv) isolates were determined in conserved (helper-component proteinase, hc; nuclear inclusion b, nib) and variable (first protein, p1; third protein, p3; and coat protein, cp) regions of the potyviral genome in order to investigate the intraspecies molecular diversity of ymv. multiple sequence alignments and pairwise comparisons were used to quantify the sequence polymorphism in these regions. two levels of diversity were ... | 1997 | 9191916 |
| long sequences in the 5' noncoding region of plum pox virus are not necessary for viral infectivity but contribute to viral competitiveness and pathogenesis. | the 5'-terminal 31 nucleotides of the 146-nucleotides-long 5' noncoding region of plum pox potyvirus (ppv) are highly conserved in all the members of the potyvirus genus. to map the sequences of the 5' noncoding region that are necessary in vivo for infectivity, we have constructed a nested set of substitution and deletion mutants. while we were not able to infect nicotiana clevelandii plants with full-length ppv transcripts bearing mutations in the 5'-terminal 35 nucleotides of the viral genome ... | 1997 | 9201225 |
| agenesis of the corpus callosum associated with paroxysmal hypothermia: shapiro's syndrome. | spontaneous recurrent hypothermia and hyperhidrosis associated with agenesis of the corpus callosum was first described by shapiro and plum in 1967. since then, several cases with similar symptoms (now known as shapiro's syndrome or spontaneous periodic hypothermia) have been described. we report another case of this syndrome in a 21-year-old-man, and discuss possible pathogenetic mechanisms and therapeutic approaches. | 1997 | 9038041 |
| serological characterization of hungarian plum pox virus isolates. | three hungarian (no. 2, 4 and 9), and a moldavian (k) plum pox virus isolates were compared with a characterized spanish isolate (5.15) by rt-pcr, elisa, dot-blot and western blot analysis. monoclonal antibodies prepared against the external, intermediate and internal sequences of the coat protein of the spanish isolate were able to differentiate the four isolates. hungarian isolate no. 2 proved to be serologically identical to the spanish isolate, while no. 4 showed appreciable differences and ... | 1997 | 9232895 |
| a case-control study of risk factors of nasopharyngeal carcinoma. | risk factors and nasopharyngeal carcinoma (npc) in residents of guangzhou city were investigated in a case-control study: 104 cases were compared with an equal number of age, sex, and neighborhood-matched controls. the results of multiple conditional regression of logistic model showed that plum vegetable (adjusted or = 1.81, 95% ci 1.01-3.33), preserved prune (adjusted or = 2.95, 95% ci 1.04-8.41), no separate kitchen (if >35 years, adjusted or = 1.98, 95% ci 1.24-3.75), kitchen range without c ... | 1997 | 9233826 |
| design of a solubilization pathway for recombinant polypeptides in vivo through processing of a bi-protein with a viral protease. | an artificial maturation pathway for increasing the solubility in vivo of recombinant proteins overproduced in escherichia coli is reported, which is based on the proteolytic processing of viral polyproteins. the gene product of interest is expressed as a fusion to a heterologous moiety (i.e. the maltose binding protein, male) in order to increase the overall solubility of the hybrid. the hinge region between the two fusion partners contains a cleavage site for the nia protein, a very specific p ... | 1997 | 9278287 |
| specific detection of d- and m-isolates of plum pox virus by immunoenzymatic determination of pcr products. | molecular techniques based on the polymerase chain reaction (pcr) can provide rapid and sensitive diagnosis of plum pox virus (ppv), the causal agent of the devastating 'sharka' disease of stone fruit trees. the present study compared routine polymerase chain reaction (pcr) procedures against a new system, pcr-elisa (boehringer mannheim), which enables immunoenzymatic detection of pcr products. the results show that this hybridisation system ensures fast and more sensitive detection of ppv assoc ... | 1997 | 9300377 |
| cap-independent leaky scanning as the mechanism of translation initiation of a plant viral genomic rna. | the genome of plum pox virus contains a single open reading frame that is translated into a large polyprotein. although the open reading frame starts at nucleotide 36 (36aug), it is translated from the second, 147aug, which is in a more favourable context for translation initiation. we have carried out in vitro translation and transient expression analysis in protoplasts of a nested set of substitution and deletion mutants, and the results show that no internal structure in the 5' noncoding regi ... | 1997 | 9349492 |
| cloning, sequencing, and expression of the mig gene of mycobacterium avium, which codes for a secreted macrophage-induced protein. | mycobacterium avium is an intracellular pathogen that has evolved to be a frequent cause of disseminated infection in immunocompromised patients. although these bacilli are readily phagocytized, they are able to survive and even multiply within human macrophages. the process whereby mycobacteria circumvent the lytic functions of the macrophages is currently not well understood, but this is a key aspect in the pathogenicity of all pathogenic mycobacteria. previously, we identified a gene in m. av ... | 1997 | 9353032 |
| the motif v of plum pox potyvirus ci rna helicase is involved in ntp hydrolysis and is essential for virus rna replication. | the plum pox potyvirus (ppv) protein ci is an rna helicase whose function in the viral life cycle is still unknown. the ci protein contains seven conserved sequence motifs typical of rna helicases of the superfamily sf2. we have introduced several individual point mutations into the region coding for motif v of the ppv ci protein and expressed these proteins in escherichia coli as maltose binding protein fusions. mutations that abolished rna helicase activity also disturbed ntp hydrolysis. no mu ... | 1997 | 9358154 |
| simultaneous detection and typing of plum pox potyvirus (ppv) isolates by heminested-pcr and pcr-elisa. | two techniques for simultaneous detection and typing of plum pox potyvirus (ppv) isolates belonging to the d or m serotypes, heminested pcr (h-pcr) and pcr-elisa, have been developed. ten ppv isolates typed using ppv-d and ppv-m specific monoclonal antibodies by elisa-dasi were used to validate these two methods. the results obtained show a complete coincidence of the nucleic acid-based techniques with the serological data. when serial dilutions of infected plant extracts were assayed, h-pcr and ... | 1997 | 9389402 |
| hop stunt viroid (hsvd) sequence variants from prunus species: evidence for recombination between hsvd isolates. | hop stunt viroid (hsvd) is able to infect a number of herbaceous and woody hosts, such as grapevine, citrus or prunus plants. previous phylogenetic analyses have suggested the existence of three major groups of hsvd isolates (plum-type, hop-type and citrus-type). the fact that these groups often contain isolates from only a limited number of isolation hosts prompted the suggestion that group-discriminating sequence variations could, in fact, represent host-specific sequence determinants which ma ... | 1997 | 9400968 |
| diversity of opines and opine-catabolizing bacteria isolated from naturally occurring crown gall tumors. | the diversity of opines from 43 naturally occurring crown gall tumors on several plant species was analyzed for the presence of agropine, chrysopine, iminodiacid, an unidentified leucinopine-like iminodiacid (ida-b), mannopine, octopine, nopaline, dl- and ll-succinamopine, leucinopine and heliopine. opine utilization patterns of agrobacteria and fluorescent pseudomonads resident in a tumor were then analyzed and compared for agreement with the opine isolated from that tumor. nopaline was the mos ... | 1997 | 16535484 |
| influence of arbuscular mycorrhizae and phosphate fertilization on shoot apical growth of micropropagated apple and plum rootstocks. | we studied the effects of phosphate fertilization and inoculation with the arbuscular mycorrhizal fungi glomus mosseae (nicol. and gerd.) gerdmann and trappe, glomus intraradices schenck and smith or glomus viscosum nicolson on shoot apical growth of plantlets that had been micropropagated from mm 106 apple (malus pumila l.) and mr.s. 2/5 plum (prunus cerasifera ehrh.) rootstocks. unfertilized and non-mycorrhizal plantlets showed no apical growth during the post in vitro acclimation phase, where ... | 1996 | 14871682 |
| the rna helicase ci from plum pox potyvirus has two regions involved in binding to rna. | the plum pox virus (ppv) protein ci is an rna helicase, whose function in the virus replication is still unknown. recently, an rna binding domain was mapped to a region of the ci protein that includes the arginine-rich motif vi typical of rna helicases of the superfamily sf2. in the present study, a second region involved in rna binding activity of the ci protein has been identified. northwestern assays with a series of maltose-binding protein fusions that contain different ci fragments showed t ... | 1996 | 8690088 |
| [polyostotic hyperostosis in a plum-headed parakeet (psittacula cyanocephala l., 1766]. | with emphasis on a case report, the clinical and pathological findings commonly associated with polyostotic hyperostosis as well as its etiology are discussed, and the available literature is reviewed. the diagnostic specifics of the disease are detailed, and a survey of possible differential diagnoses is given. | 1996 | 8720953 |
| cold calcium signaling in arabidopsis involves two cellular pools and a change in calcium signature after acclimation. | cold shock elicits an immediate rise in cytosolic free calcium concentration ([ca2+]cyt) in both chilling-resistant arabidopsis and chilling-sensitive tobacco (nicotiana plumbaginifolia). in arabidopsis, lanthanum or egta caused a partial inhibition of both cold shock [ca2+]cyt elevation and cold-dependent kin1 gene expression. this suggested that calcium influx plays a major role in the cold shock [ca2+]cyt response and that an intracellular calcium source also might be involved. to investigate ... | 1996 | 8721751 |
| frequent occurrence of recombinant potyvirus isolates. | we have performed a systematic search for recombination in the region encoding coat protein and the 3' non-translated region in natural isolates of potyviruses, the largest group of plant rna viruses. the presence of recombination, and the localization of the cross-over points, were confirmed statistically, by three different methods. recombination was detected or suspected in 18 out of 109 potyvirus isolates tested, belonging to four out of eight virus species, and was most prevalent in potato ... | 1996 | 8760448 |
| first findings of plum pox virus in walnut trees (juglans regia l.) | plum pox virus (ppv) was transmitted from infected buds and leaves of walnut tree (juglans regia l.) on the following herbaceous indicators: chenopodium foetidum schrad., nicotiana bigelovii var. quadrivalvis fuchs., n. clevelandii x n. glutinosa. a positive elisa reaction with antisera against ppv was obtained from infected buds and leaves of juglans regia l. and from attacked leaves of indicator plants. | 1996 | 8886101 |
| epidemiologic association between antibody titres against bovine virus diarrhoea virus, rinderpest disease virus and infectious bovine rhinotracheitis virus in a buffalo herd. | the association between antibody titres against bovine virus diarrhoea virus (bvdv), infectious bovine rhinotracheitis virus (ibrv) and rinderpest disease virus (rpdv) in buffaloes was investigated in a cross-sectional study. thirty-six lactating buffaloes from a herd in landhi cattle colony, karachi were bled and serum samples subjected to a micro-neutralisation test at the plum island animal disease center, usa, to categorise each buffalo either as positive or negative against each of 3 viral ... | 1996 | 8888525 |
| a nurse with anaphylaxis to stone fruits and latex sensitivity: potential diagnostic difficulties to consider. | there have been several recent reports describing a cross-reactivity between latex and antigens derived from a number of fruits, including those of the stone fruit family. the relationships between the allergic reactions to the antigens from these plant products are still being defined. | 1996 | 8970444 |
| physico-chemical and sensory properties of formulated syrup from black plum (vitex doniana) fruit. | the fresh ripe fruit pulp and syrup, produced using the open pan evaporator, of vitex doniana were analysed physico-chemically. results show that the edible pulp of the ripe fruit is fairly rich in vitamin c (18.1 mg/100 ml) but acidic (ph 4.38) and poor in protein (0.82%) and oil (0.56%). the syrup yielded a de value of 52, total dissolved solids content of 67 degrees brix and a refractive index of 1.4762 at 20 degrees c. the syrup was moderately viscous (2.1 poiseuille), acid (ph 4.45) but rel ... | 1996 | 8983056 |
| print-capture pcr: a simple and highly sensitive method for the detection of plum pox virus (ppv) in plant tissues. | 1996 | 8668554 | |
| fungi associated with post-harvest rot of black plum (vitex doniana) in nigeria. | the fungi associated with rot of vitex doniana fruits (blackplum) were isolated and identified. aspergillus niger, botryodiplodia theobromae, candida spp. penicillium chrysogenum, rhizopus stolonifer, fusarium pallidoroseum f. oxysporum and mucor mucedo were the primary rot causing fungi in contrast to cladosporium herbarum and mucor circinelloides which were just present as secondary colonizers. the rot fungi penetrated mainly through wounds and bruises on the surface of fruits. mature green fr ... | 1996 | 9208478 |
| prunus rootstock evaluation to root-knot and lesion nematodes in spain. | two screening and one resistance verification trial involving 20 prunus rootstocks were conducted under greenhouse conditions against meloidogyne spp. and pratylenchus vulnus. most of the rootstocks were experimental genotypes or new commercial peach and plums of spanish and french origin. nearly all are interspecific hybrid rootstocks. in the first trial, the rootstocks bruce, cadaman, mirac, g x n no. 15, cachirulo x (g x n no. 9), and p. myra x peach were immune or resistant to a mixture of s ... | 1996 | 19277184 |
| effect of cutting age on the resistance of prunus cerasifera (myrobalan plum) to meloidogyne arenaria. | the response of softwood cuttings of myrobalan plum infested after 50 and 105 days with 3,000 second-stage juveniles (j2) of meloidogyne arenaria was compared to 15-month-old hardwood cuttings in 13 genotypes ranging from highly resistant to susceptible. gall index and number of galls were recorded 30 days after infestation. fifty-day-old cuttings rooted in perlite developed many rootlets, but had only incipient galls after infestation. in sand, rooting of 50-day-old cuttings not treated with in ... | 1995 | 19277333 |
| a modified device for long term sampling of parotid saliva in various experimental conditions. | a parotid cup has been widely used as an intraoral device for the collection of parotid saliva. however, in long duration sampling, the cup tends to disconnect under circumstances such as mastication, sleep or physical exercise. we have designed a modified device of parotid cup for saliva collection to overcome these unfavorable conditions. the parotid cup was modified by fixing it to the bite-plate appliance which was adjusted individually to the occlusal plane in order to avoid disconnection o ... | 1995 | 8689745 |
| processing of the plum pox virus polyprotein at the p3-6k1 junction is not required for virus viability. | proteolytic processing of the potyvirus polyprotein is mainly performed by the virus-encoded nia protease, whose cleavage sites are characterized by conserved heptapeptide sequences. partial processing at the cleavage site present between the p3 and 6k1 cistrons by the plum pox potyvirus (ppv) nia protease has been previously shown to occur in vitro. we have now studied the role of polyprotein processing at the p3-6k1 junction in vivo, using a full-length ppv cdna clone. ppv mutant transcripts c ... | 1995 | 9049341 |
| unusual foreign bodies of upper gastrointestinal tract. | we report management of unusual foreign bodies of upper gastrointestinal tract, namely beer bottle cap, raisins and pistachu, mango peel, betelnut and plum seed at a university hospital in northern india. | 1995 | 8854958 |
| rna helicase activity of the plum pox potyvirus ci protein expressed in escherichia coli. mapping of an rna binding domain. | the plum pox potyvirus (ppv) cylindrical inclusion (ci) protein fused to the maltose binding protein (mbp) has been synthesized in escherichia coli and purified by affinity chromatography in amylose resin. in the absence of any other viral factors, the fusion product had ntpase, rna binding and rna helicase activities. these in vitro activities were not affected by removal of the last 103 amino acids of the ci protein. however, other deletions in the c-terminal part of the protein, although leav ... | 1995 | 7538661 |
| transmission by aphids of a naturally non-transmissible plum pox virus isolate with the aid of potato virus y helper component. | two spanish plum pox virus (ppv) isolates, 5.15 and 3.3, were used in transmission experiments involving the aphid vector myzus persicae, with woody and herbaceous host plants. these isolates differ in the size of their coat protein (cp) and sequence analysis revealed that isolate 3.3 has a 15 amino acid deletion near the n terminus of the cp, affecting the same positions as in a previously reported non-aphid-transmissible ppv isolate from germany. aphid transmission experiments showed that isol ... | 1995 | 7561767 |
| genetic relationships among strains of xylella fastidiosa from rapd-pcr data. | genetic relationships among 11 xylella fastidiosa strains isolated from mulberry, almond, ragweed, grape, plum, elm, and citrus were determined by random amplified polymorphic dna (rapd). twenty-two 10-base primers amplified a total of 77 discrete polymorphic bands. phenetic analysis based on a similarity matrix corresponded well with previous reports on x. fastidiosa rflp-based similarity relationships, indicating that rapd-pcr amplification products can be used as a reliable indicator of genet ... | 1995 | 7606188 |
| randomly amplified polymorphic dna analysis of xylella fastidiosa pierce's disease and oak leaf scorch pathotypes. | randomly amplified polymorphic dna analysis was conducted with 14 primers to 17 strains of xylella fastidiosa. there was a high degree of similarity among the seven pierce's disease (pd) strains (sxy > 0.93) and the seven oak leaf scorch (ols) strains (sxy > 0.96). however, the two groups were different, with a similarity index of 0.67, confirming the presence of a pd dna cluster and suggesting the presence of a new ols cluster. the control plum leaf scald strains (two strains) together with the ... | 1995 | 7646005 |
| expression of the plum pox coat protein gene in transgenic nicotiana tabacum plants. | plant expression vector pbi 121 containing the gene encoding coat protein of plum pox virus of the skierniewice isolate (cp ppv-s) was prepared (clone pcm1). the construct was used for transformation of nicotiana tabacum plants using an agrobacterium tumefaciens based system. about 82% of kanamycin resistant plant lines contained a transgene (the sequence of cp ppv-s) but only 81% of them actively expressed the ppv-s coat protein gene as measured by rt-pcr. | 1995 | 7653168 |
| properties of the active plum pox potyvirus rna polymerase complex in defined glycerol gradient fractions. | as a first step in the study of the replication of plum pox virus (ppv) rna, an in vitro virus-specific rna polymerase activity was characterized in a crude membrane extract (martin and garcia, 1991). in this study, we report the fractionation of the crude membrane extract by centrifugation in glycerol gradients. the sedimentation properties after different treatments of the crude extract and its insensitivity to micrococcal nuclease treatment suggest that the rna polymerase activity was localiz ... | 1995 | 7483826 |
| berenil binding to higher ordered nucleic acid structures: complexation with a dna and rna triple helix. | berenil is an antitrypanosomal agent that binds to nucleic acid duplexes. recently, we reported that this drug can bind to both dna and rna duplexes, while exhibiting properties characteristic of both intercalation and groove binding [pilch, d. s., kirolos, m. a., liu, x., plum, g. e., & breslauer, k. j. (1995) biochemistry 34, 9962-9976]. in this work, we use spectroscopic, calorimetric, and hydrodynamic techniques to demonstrate that berenil also can bind to dna and rna triplexes. our results ... | 1995 | 8519768 |
| localization of fruit tree viruses by immuno-tissue printing in infected shoots of malus sp. and prunus sp. | immuno-tissue printing protocols for the localization of apple chlorotic leaf spot virus (aclsv), stem grooving virus (sgv) and plum pox virus (ppv) in shoots of prunus and malus in vitro have been established for routine diagnosis in a virus elimination program. since these viruses belong to different virus genera, the protocols were adapted according to the properties of the virus under investigation. accumulation of aclsv was highest in the base of the stem and decreased towards the apex of t ... | 1995 | 8537455 |
| effects of land use on the degradability of dissolved organic matter in three watersheds of the plum island sound estuary. | 1995 | 8541418 | |
| production and characterization of monoclonal antibodies to plum pox virus and their use in differentiation of mediterranean isolates. | monoclonal antibodies (mabs) specific to plum pox virus (ppv) were prepared by fusing myeloma cell lines to spleen cells of mice immunized with purified virus, including virus prepared with protease inhibitors to preserve the integrity of the coat protein (cp). the characterized mabs could be used in elisa to differentiate several mediterranean ppv isolates differing in their geographical origin and cp size. at least seven antigenic sites could be established based on the recognition pattern and ... | 1994 | 7526822 |
| [long-term survival following excision of a neck carotid body tumor]. | farmer with a jugular tumor on the right side and suffering from head and shoulder aches, and epileptic seizures during sleep was admitted to a district hospital and underwent surgery. partial anesthesia was applied and neck vascular area exposed. intraoperatively a tumor of plum size was seen which constricted carotid artery bifurcation with its branches. the pulse in the internal carotid artery was impalpable. carotid artery was clamped, and conversation with the patient begun to find out whet ... | 1994 | 7708559 |
| sensitive non-radioactive nucleic acid hybridization assay for plum pox virus detection. | a new non-radioactive sandwich hybridization assay was designed to simplify the analysis of a large number of plant samples. plant material was homogenized in 0.5% sds and added directly to the hybridization reaction, in which a pair of identifying probes were used. one of the probes was biotinylated capture rna specific for plum pox virus (ppv) strain sk-68; the other rna probe was synthesized from a plasmid bearing the adjacent sequence of this strain and was labelled with digoxigenin (dig). b ... | 1994 | 7709075 |
| [occurrence of aluminum in some foodstuffs]. | results of aluminium determinations in breads and biscuits as well as in fish preserves, vegetables, strawberries and teas including fruit-herb teas are presented. also the percent of extraction of aluminium of various kind of tea leaves into the infusions was determined. the commercially available sample of foods was used for investigation. aluminium was determined by using two methods: a spectrophotometric extraction method with-hydroxyquinoline and less sensitive aas method with nitrous oxide ... | 1994 | 7777774 |
| development and preliminary testing of the neurological assessment instrument. | the purpose of this study was to test a neurological assessment instrument (nai), used to assess neurological function in adults with acute brain lesions. instrument items were designed to reflect different aspects of neurological function and assess consciousness along the arousal/awareness continuum as described by plum and posner. content of the nai was validated by 10 neuroscience expert physicians, nurses and speech pathologists. reliability was tested with one pair of raters on 39 neurolog ... | 1994 | 7829918 |
| simple and rapid preparation of infected plant tissue extracts for pcr amplification of virus, viroid, and mlo nucleic acids. | a rapid, simple method for preparing plant tissues infected with viruses, viroids, or mlos using a commercial product known as gene releaser is described. the gene releaser polymeric matrix method produced plant extracts suitable for pcr amplification without the use of organic solvents, ethanol precipitation, or additional nucleic acid purification techniques. modification of maceration methods and/or extraction buffers resulted in the pcr amplification of potato spindle tuber, apple scar skin, ... | 1994 | 7868647 |
| nucleotide sequence of the coat protein gene of the skierniewice isolate of plum pox virus (ppv). | the coat protein (cp) gene of the skierniewice isolate of plum pox virus (ppv-s) has been amplified using the reverse transcription--polymerase chain reaction (rt-pcr), cloned and sequenced. the nucleotide sequence of the gene and the deduced amino-acid sequence of ppv-s cp were compared with those of other ppv strains. the nucleotide sequence showed very high homology to most of the published sequences. the motif: asp-ala-gly (dag), important for the aphid transmissibility, was present in the a ... | 1994 | 7913278 |
| allergenic cross-reactivity among peach, apricot, plum, and cherry in patients with oral allergy syndrome: an in vivo and in vitro study. | oral allergy syndrome in response to fruits and vegetables frequently occurs as clusters of hypersensitivity to members of the same botanical family, for which the immunologic basis lies in a number of common allergens, most of them still unidentified. | 1994 | 7930303 |
| nectar feeding by the early-spring mosquito aedes provocans. | nectar feeding by males and females of the mosquito aedes provocans was studied at a site near belleville, ontario, canada. canada plum, prunus nigra, and especially pin cherry, p. pensylvanica, bloomed contemporaneously with the emergence of ae. provocans and were important nectar sources for adult mosquitoes during their first week of life. blossoms of p. pensylvanica shielded for 24 h from foragers produced an average of 0.14 mg of sugar (approximately 2.3j). this nectar was avidly sought by ... | 1994 | 7949310 |
| fibre constituents of some foods. | some plant foods viz. bottlegourd, carrot, cauliflower, cabbage, green bengalgram, pea, apple, plum, guava, karonda, blackgram husk and lentil husk were analysed for their dietary fibre components. the total dietary fibre contents of these foods varied from 14.68 to 78.21 percent on dry matter basis. as compared to fruits and vegetables, the husks had higher amount of total dietary fibre. cellulose represented as the major fibre constituent in most of the foods whereas, husks were observed to be ... | 1994 | 7971775 |
| parkinson and the plum tree. | 1994 | 7986170 | |
| effect on lipopolysaccharide structure of aeration during growth of a plum isolate of pseudomonas syringae pv. morsprunorum. | the composition of lipopolysaccharide (lps) extracted with aqueous phenol from a virulent english plum isolate of pseudomonas syringae pv. morsprunorum varied according to the partial pressure of oxygen (po2) in the culture medium at the time of harvest. when po2 was low, the organism grew slowly and produced smooth lps bearing rhamnan sidechains. as po2 was raised, the rate of growth increased and smooth lps was replaced by a rough species deficient in rhamnose, which co-extracted with a d-gluc ... | 1994 | 8075800 |
| tissue level compartmentation of (r)-amygdalin and amygdalin hydrolase prevents large-scale cyanogenesis in undamaged prunus seeds. | plum (prunus domestica) seeds, which contain the cyanogenic diglucoside (r)-amygdalin and lesser amounts of the corresponding monoglucoside (r)-prunasin, release the respiratory toxin hcn upon tissue disruption. amygdalin hydrolase (ah) and prunasin hydrolase (ph), two specific [beta]-glucosidases responsible for hydrolysis of these glucosides, were purified to near homogeneity by concanavalin a-sepharose 4b and carboxymethyl-cellulose chromatography. both proteins appear as polypeptides with mo ... | 1994 | 12232058 |
| reproductive fitness and random amplified polymorphic dna variation among isolates of pratylenchus vulnus. | the reproductive fitness of seven isolates of pratylenchus vulnus from different geographical areas and hosts was assessed in monoxenic cultures (carrot), and greenhouse cultures (plum, sour orange, and quince). the genetic makeup of the different isolates was compared by random amplified polymorphic dna (rapd-pcr). the apple (pvap-s) and apricot (pvat-f) isolates reproduced less in monoxenic cultures than the rose (pvro-s) and walnut (pvwa-a and pvwa-u) isolates. on plum, the rose isolate (pvro ... | 1994 | 19279892 |
| transgenic plums (prunus domestica l.) express the plum pox virus coat protein gene. | plum hypocotyl slices were transformed with the coat protein (cp) gene of plum pox virus (ppv-cp) following cocultivation with agrobacterium tumefaciens containing the plasmid pga482gg/ppvcp-33. this binary vector carries the ppv-cp gene construct, as well as the chimeric neomycin phosphotransferase and β-glucuronidase genes. integration and expression of the transferred genes into regenerated plum plants was verified through kan resistance, gus assays, and pcr amplification of the ppv-cp gene. ... | 1994 | 24194220 |
| comparative sequence analysis of four complete primary structures of plum pox virus strains. | the complete nucleotide sequence of plum pox virus (ppv) strain sk 68 was determined from a series of overlapping cdna clones. the exact 5' terminus was determined by direct rna sequencing. the rna sequence was 9786 nucleotides in length, excluding a 3' terminal poly(a) sequence. the large open reading frame starts at nucleotide position 147 and is terminated at position 9568. comparison of cistrons from other plum pox virus strains with those predicted for the sk 68 strain indicated the same ge ... | 1993 | 8122394 |
| survey of mycoflora and mycotoxins of some dried fruits in egypt. | fifty-five species and two varieties appertaing to 23 genera were collected and identified from 4 samples of dried fig and 3 dried samples of each of apricot, plum and raisin. forty-nine species and two varieties belonging to 20 genera were isolated on 1% glucose-czapek's while 31 species and one variety belonging to 16 genera were isolated on 40% sucrose-czapek's agar medium at 28 +/- 2 degrees c. penicillium, aspergillus and cladosporium were the common genera on the two types of media used. a ... | 1993 | 8229671 |
| treatment of facial spasm by plum-blossom needle tapping and cupping therapy. | 1993 | 8246597 | |
| care allegedly provided without proper supervision. case in point: raicevich v. plum creek medical p.c. 918 f. supp. 2d 929--co (1993). | 1993 | 8265797 | |
| monoclonal antibodies suitable for plum pox virus determination. | monoclonal antibodies (mabs) to plum pox virus (ppv) were obtained after immunization of balb/c mice with purified ppv-w isolate. spleen cells from a mouse showing a high serum titer were used for fusion with sp2/0-ag14 myeloma cells. culture supernatants were screened for specific antibody production against ppv-w isolate using indirect elisa. a total of six stable hybridoma lines producing mabs of igg class were obtained. all four ppv isolates tested (w, a, d and m) can be distinguished by the ... | 1993 | 8314598 |
| systemic phaeohyphomycosis caused by exophiala dermatitidis. | we report a case of systemic phaeohyphomycosis due to exophiala dermatitidis in a 24-year-old man. at the age of 17, the patient had noticed cervical swellings. on palpation at his initial examination, more than a dozen firm lymph nodes between 1 and 5 cm in diameter were found on both sides of the neck and in both axillae. examination of biopsy specimens of lymph nodes revealed pale brown hyphae in granulomatous lesions and exophiala dermatitidis was isolated from mycological cultures of the lo ... | 1993 | 8316255 |
| inhibitory effects of human cystatin c on plum pox potyvirus proteases. | the effect of different protease inhibitors on the proteolytic processing of the plum pox potyvirus (ppv) polyprotein has been analyzed. human cystatin c, an inhibitor of cysteine proteases, interfered with the autoprocessing of the viral papain-like cysteine protease hcpro. unexpectedly, it also had an inhibitory effect on the autocatalytic cleavage of the nla protease which, although it has a cys residue in its active center, has been described as structurally related to serine proteases. othe ... | 1993 | 8343605 |
| complete nucleotide sequence of the genome of an apple isolate of apple chlorotic leaf spot virus. | the complete nucleotide sequence of the genome of an apple isolate of apple chlorotic leaf spot virus (aclsva) was determined. the genome is 7552 nucleotides excluding the poly(a) tail and contains three open reading frames (orfs 1, 2 and 3), encoding proteins with m(r) values of 216,503 (216.5k), 50,453 (50.4k) and 21,394 (21.4k), respectively. nucleotide sequence comparisons between aclsv-a and the previously sequenced aclsv from plum (aclsv-p) showed that the sequence identity at the nucleoti ... | 1993 | 8376968 |
| 3'-terminal sequence of the plum pox virus ps and ŏ6 isolates: evidence for rna recombination within the potyvirus group. | the sequence of the 3'-terminal 1768 nucleotides of the ps and ŏ6 isolates of plum pox virus (ppv) has been determined and compared with that of the equivalent regions of other ppv isolates sequenced previously. the sequenced region is part of the ppv open reading frame encoding the last 186 amino acids of the nib protein and the coat protein (cp, 330 amino acids), followed by a non-coding region of 220 nucleotides and a poly(a) tail. ppv-ps and ppv(-)ŏ6, just like ppv-el amar, show rather high ... | 1993 | 8445362 |
| immunodetection of the plum pox virus helper component in infected plants and expression of its gene in transgenic plants. | tobacco plants (nicotiana tabacum cv. xanthi) have been transformed via agrobacterium tumefaciens vectors, with cdnas corresponding to the plum pox virus (ppv) cistron 2 encoding helper component (hc-pro) and with the first two and half cistrons of the ppv genome. presence of the hc-pro in ppv-infected plants and transgenic plants transformed with the gene coding for this protein was investigated using specific polyclonal antibodies produced against the ppv hc-pro. the results suggest that two p ... | 1993 | 8517789 |
| [antimutagenic substances in the armeniacae semen and persicae semen]. | using the ames/salmonella/microsome assay, we examined the antimutagenic effect of the hexane extract of armeniacae semen (apricot (prunus armeniaca l.) seed), persicae semen (peach (p. persica bat.) seed), and seeds of cherry (p. avium l.), plum (p. salicina lindle) and almond (p. dulcis mill). hexane extracts of armeniacae semen and persicae semen inhibited the mutagenicity of benzo[a]pyrene (b[a]p), but those of seeds of cherry, plum and almond did not. the mutagenicities of 3-amino-1,4-dimet ... | 1992 | 1294719 |
| [the type of coma as prognostic indicator in severe cranial trauma in childhood]. | various methods are used by neurologists to evaluate posttraumatic brain damage. the most important and reliable are the length of posttraumatic amnesia and coma. in previous papers we have already described the value of the type of coma in the prognosis of serious head injury in childhood (baracchini-muratorio et al. 1985; pruneti et al. 1985). in this study, 30 children (aged 6-12 years) with serious closed head injury and subsequent coma were evaluated. the children were divided into two grou ... | 1992 | 1294915 |
| construction of a chimeric viral gene expressing plum pox virus coat protein. | the capsid-encoding gene of plum pox virus (ppv) was fused with the leader sequence of the coat protein mrna (cp) of tobacco mosaic virus by a novel mutagenesis technique which involves reverse transcription of minus-strand rna [synthesized by in vitro transcription of a double-stranded (ds) cdna clone], using an ad hoc synthetic oligodeoxynucleotide as primer. the resulting cdna was rendered ds and cloned into the plasmid, pbluescribe m13+. transcription of this chimeric construction produced r ... | 1992 | 1398133 |
| advanced licensure: personal plum or public shield? | 1992 | 1408282 | |
| the complete nucleotide sequence of pepper mottle virus genomic rna: comparison of the encoded polyprotein with those of other sequenced potyviruses. | the complete nucleotide sequence of a pepper mottle virus isolate from california (pepmov c) has been determined from cloned viral cdnas. the pepmov c genomic rna is 9640 nucleotides excluding the poly(a) tail and contains a long open reading frame starting at nucleotide 168 and potentially encoding a polyprotein of 3068 amino acids. comparison of the pepmov c presumptive polyprotein with those of other sequenced members of the potyvirus group, including tobacco etch virus (tev), tobacco vein mo ... | 1992 | 1413501 |
| a highly sensitive immunocapture polymerase chain reaction method for plum pox potyvirus detection. | a highly sensitive assay, based on polymerase chain reaction amplification of cdna synthesized from the viral rna of antibody-captured viral particles, has been developed for plum pox potyvirus (ppv) detection. the reaction, called immunocapture/pcr (ic/pcr), yields a specific 243-bp product. the immunocapture step, by allowing the use of large sample volumes and by the viral particle prepurification it achieves, dramatically increases the sensitivity of the assay. as few as 8000 target viral pa ... | 1992 | 1430063 |
| treatment of neurasthenia with electric plum-blossom needle therapy and observations of body surface reactions. | 1992 | 1495328 | |
| observations on the efficacy in treating scleritis by plum-blossom needle. | 1992 | 1495335 | |
| advanced licensure: personal plum or public shield? | 1992 | 1501849 | |
| infectious in vivo transcripts of a plum pox potyvirus full-length cdna clone containing the cauliflower mosaic virus 35s rna promoter. | a full-length cdna clone of an aphid non-transmissible isolate of plum pox potyvirus (ppv) was rendered biologically active when placed under the control of the cauliflower mosaic virus 35s rna promoter and the nopaline synthase polyadenylation signal. the cdna was constructed so that the exact 5' end of the ppv rna was present at the transcription initiation site. inoculation of plasmid dna onto nicotiana benthamiana led to systemic infection, whereas local lesions were produced in chenopodium ... | 1992 | 1545225 |
| proteolytic processing of the plum pox potyvirus polyprotein by the nia protease at a novel cleavage site. | the expression of potyvirus genomic rna takes place through translation of its unique long and functional open reading frame into a large polyprotein that undergoes extensive proteolytic processing. most of the cleavages are performed by the virus-encoded nia protease, which cuts the polyprotein at defined sites that are characterized by conserved heptapeptide sequences. we have demonstrated in vitro cleavage activity by the plum pox potyvirus (ppv) nia protease at a novel site, previously ident ... | 1992 | 1585641 |
| nucleotide sequence of the 3'-terminal region of potato virus a rna. | the sequence of the 3'-terminal region of the genome of the potato virus a (pva) was obtained from two independent cdna clones. this sequence is 1383 nucleotides long and contains an open reading frame of 1178 nucleotides, ending with the translation termination codon taa and followed by untranslated region of 205 nucleotides. since the n-terminal amino acid of the coat protein of pva was blocked, the position of the putative coat protein cleavage site has been deduced by searching for consensus ... | 1992 | 1604933 |
| [struma carcinoid tumor within a dermoid cyst as an incidental finding in cesarean section]. | a 25-year-old primigravida with breech presentation was admitted near term. during caesarean section, a dermoid cyst of the right ovary was detected. the dermoid cyst was enucleated, preserving a plum-sized ovary. histology revealed a strumal carcinoid within the excised dermoid cyst. from the histological point of view it was classified as a tumour of low malignancy. the clinical study provided no evidence of any metastases. on the 14th postoperative day the patient could be discharged from hos ... | 1992 | 1607120 |
| shoulder dystocia: when to use suprapubic or fundal pressure. | "the delivery of the head with or without forceps may have been quite easy, but more commonly there has been a little difficulty in completing the extension of the head. the hairy scalp slides out with reluctance. when the forehead has appeared it is necessary to press back the perineum to deliver the face ... time passes. the child's head becomes suffused. it endeavors unsuccessfully to breathe. abdominal efforts by the mother or by her attendants produce no advance; gentle head traction is equ ... | 1992 | 1738307 |
| carbon and nitrogen partitioning in peach/plum grafts. | modifications in root-shoot relationships induced by graft incompatibility were studied in peach/plum graft combinations by means of carbohydrate and nitrogen analyses and isotope labeling. mobilization of stored carbon, phloem transport of carbon, and mobilization, assimilation and distribution of nitrogen were studied in one compatible peach/plum graft (prunus persica l. batsch cv. springtime grafted on prunus cerasifera l. ehrh cv. myrobalan p 2032) and one incompatible graft (prunus persica ... | 1992 | 14969877 |
| comparison of drought resistance among prunus species from divergent habitats. | root and shoot characteristics related to drought resistance were compared among cultivated peach (prunus persica (l.) batsch.), p. andersonii (nevada desert almond), p. besseyi (western sand cherry), p. maritima (beach plum), p. subcordata (sierra or pacific plum), and p. tomentosa (nanking cherry). in all species, shoot characteristics were more closely associated with drought adaptation than root characteristics. the most xeric species, p. andersonii, had the lowest specific leaf area, smalle ... | 1992 | 14969942 |
| host range of a population of pratylenchus vulnus in commercial fruit, nut, citrus, and grape rootstocks in spain. | in a host-range study carried out under greenhouse conditions, a total of 37 commercial fruit tree, grape, and citrus rootstocks were tested for their reaction to a population of the lesion nematode, pratylenchus vulnus, in spain. twenty-five rootstocks had a pf/pi > 1.5. these included almond (desmayo rojo, 1143), apple (em-9, em-106), avocado (hass), cherry (santa lucia 64, camil, m x m 14, masto de montafiana), grape (41-b, fercal, ritcher 110), hazelnut (pauetet), loquat (nadal), peach (mont ... | 1992 | 19283047 |
| host suitability of eight prunus spp. and one pyrus communis rootstocks to pratylenchus vulnus, p. neglectus, and p. thornei. | the effects of pratylenchus vulnus on rootstocks of eight commonly used prunus spp. and one pyrus communis were evaluated under greenhouse conditions during a 15-month period. in a first experiment, two almonds (moncayo and garrigues), one peach (gf-305), and two peach-almond hybrids (gf-677 and adafuel) inoculated with 2,000 nematodes per plant proved to be good hosts of p. vulnus. highest (p < 0.05) numbers of nematodes per gram of fresh root weight were recovered from adafuel and gf-677. root ... | 1991 | 19283165 |
| the effect of different atmospheric ozone partial pressures on photosynthesis and growth of nine fruit and nut tree species. | nursery stock of peach (prunus persica l. batsch, cv. o'henry), nectarine (p. persica l. batsch, cv. fantasia), plum (p. salicina lindel., cv. casselman), apricot (p. armeniaca l., cv. tilton), almond (p. dulcis mill., cv. nonpareil), prune (p. domestica l., cv. improved french), cherry (p. avium l., cv. bing), oriental pear (pyrus pyrifolia rehd., cv. 20th century), and apple (malus pumula mill., cv. granny smith) were planted in open-top chambers on april 1, 1988 at the university of californi ... | 1991 | 14972900 |
| marissa and alyssa--new names in transplantation frontiers. | as the national media focused attention on teenager anissa ayala who received a transplant of marrow cells "donated" by her baby sister, marissa, who was conceived for the purpose of providing the marrow, a less publicized unsuccessful transplant procedure performed on alyssa plum a month earlier probably poses a greater challenge to ethical analysis. the following articles describe both stories. | 1991 | 10111505 |
| a polymerase chain reaction assay adapted to plum pox potyvirus detection. | a sensitive, polyvalent assay based on the polymerase chain reaction (pcr) was developed for plum pox potyvirus (ppv) detection. this technique was adapted for a single tube, the chemical denaturation and reverse transcription of the viral rna followed by the pcr reaction yielding a 243-base-pair product. as few as 10 fg of purified viral rna, corresponding to approximately 2000 viral particles, were detected in plant extracts. all ppv isolates tested were amplified, and the amplified fragments ... | 1991 | 1783677 |
| novel catalytic activity associated with positive-strand rna virus infection: nucleic acid-stimulated atpase activity of the plum pox potyvirus helicaselike protein. | the cylindrical inclusion protein of potyviruses contains the so-called nucleoside triphosphate binding motif, an amino acid sequence motif present in proteins encoded by most positive-strand rna viruses, some double-strand rna viruses, apparently all groups of double-strand dna viruses, and also several single-strand dna viruses. further sequence analysis has allowed to include the cylindrical inclusion protein of potyviruses as a member of a superfamily of helicaselike proteins. in this paper ... | 1991 | 1845877 |
| nucleotide sequence of the 3'-terminal region of the rna of the el amar strain of plum pox potyvirus. | the nucleotide sequence of the 3'-terminal 4773 nucleotides of the rna of a widely divergent, aphid-transmissible strain of plum pox potyvirus isolated from egypt (ppv-el amar) was determined. the sequenced region covers the carboxy terminus of the cylindrical inclusion (ci) gene, and the putative 6k protein, the nia protease, the nib rna polymerase and the coat protein genes, linked together as one large open reading frame (orf) in a fashion similar to the canonical genomic organization of othe ... | 1991 | 1856701 |
| a pcr membrane spot assay for the detection of plum pox virus rna in bark of infected trees. | a procedure for sensitive detection of plum pox virus rna in infected bark of trees is described. the method is based on the extraction of bark material with buffer containing proteinase k followed by partial purification of rna using quiagen anion exchange resin. the rna is then reverse transcribed, the single stranded cdna is amplified by the polymerase chain reaction using biotinylated deoxynucleotides as label. the amplified cdna can subsequently be detected by spotting the reaction mixture ... | 1991 | 1864904 |
| the complete nucleotide sequence of pea seed-borne mosaic virus rna. | the complete nucleotide sequence of the rna genome of pea seed-borne mosaic virus (psbmv) was determined from cloned cdna and by direct sequencing of viral rna. the psbmv genomic sequence was determined to be 9924 nucleotides in length excluding the poly(a) tract. the rna contained an open reading frame (orf) of 9618 nucleotides with the potential to encode a polyprotein with a calculated mr of 364000 (364k). the orf was flanked by a 5' untranslated leader sequence of 143 nucleotides and a 3' un ... | 1991 | 1940858 |
| plum pit ileus: a case report. | 1991 | 1944114 | |
| rat preference for food-related odors. | preferences for food-related odors and the effects of fasting on those preferences were investigated during rat bar pressing for brief odor presentation. a rat was housed in an equilateral octagonal cage and had free access to food and water, except during fasting. among 8 food-related odor substances (black pepper, cheese, coffee, milk, nut, peppermint, plum and orange), black pepper, milk and coffee were most preferred, and cheese was least preferred, but even the bar pressing rate for cheese ... | 1991 | 1959035 |
| identification of the initiation codon of plum pox potyvirus genomic rna. | the expression of plum pox potyvirus (ppv) genomic rna takes place through translation of its unique long and functional open reading frame (orf) into a large polyprotein that undergoes extensive proteolytic processing. in this paper we show that the aug recognized as the initiation codon of the ppv orf by in vitro translation systems is the one found at nucleotide position 147, in spite of the presence at position 36 of an in-phase aug that marks the start of the orf. deletion of a substantial ... | 1991 | 1962436 |
| proteolytic activity of plum pox virus-tobacco etch virus chimeric nia proteases. | plasmids encoding chimeric nia-type proteases made of sequences from the potyviruses plum pox virus (ppv) and tobacco etch virus (tev) have been constructed. their proteolytic activity on the large nuclear inclusion protein (nib)-capsid protein (cp) junction of each virus was assayed in escherichia coli cells. the amino half of the protease seemed to be involved neither in the enzymatic catalysis nor in substrate recognition. in spite of the large homology among the ppv and tev nia-type protease ... | 1991 | 2015911 |
| plum pox potyvirus rna replication in a crude membrane fraction from infected nicotiana clevelandii leaves. | in vitro synthesis of plum pox potyvirus (ppv)-specific nucleic acid has been measured in a crude fraction prepared from leaves of ppv-infected nicotiana clevelandii plants. using alkali and dnase treatments, the synthesized nucleic acid was shown to be rna. the electrophoretic mobility and the differing sensitivity to rnase at high and low salt concentrations allowed the identification of in vitro products probably corresponding to replicative form and replicative intermediate rna, as well as t ... | 1991 | 2016593 |
| catalytic antisense rnas produced by incorporating ribozyme cassettes into cdna. | a simple strategy is described for the generation of catalytic hammerhead-type ribozymes (rz) that can be used as highly specific endoribonucleases to cleave a particular target rna. the technique requires that a cloned cdna fragment is available which encodes at least a part of the target rna. about 25 different restriction recognition sequences can be utilized to incorporate specifically designed dna cassettes into the cdna. besides some nucleotides which are specific for a certain restriction ... | 1991 | 1660835 |
| phylogenetic relationships in the stone fruit group of prunus as revealed by restriction fragment analysis of chloroplast dna. | in order to clarify the genetic relationships among stone fruits, a restriction fragment analysis of chloroplast dnas (cpdnas) was applied to cultivated prunus species, whose genetic diagnoses are difficult because of their heterogeneity and long life span. chloroplast dnas (cpdnas) were extracted from leaves of nine stone fruit accessions covering six species of prunus. a restriction fragment analysis was conducted by gel electrophoresis after digestion with these endonucleases. the genome size ... | 1991 | 1676591 |
| use of monoclonal antibodies for the identification of different antigenic domains in apple chlorotic leaf spot virus. | thirteen monoclonal antibodies (mabs) specific for apple chlorotic leaf spot virus (aclsv), produced by the somatic cell hybridization technique, were used to investigate the antigenic structure of the virus. epitope specificity studies showed that these mabs defined in aclsv particles seven independent antigenic domains, representing at least eight distinct epitopes. one of them was present only in virions and not in dissociated subunits. it appeared that the interaction between a mab and the v ... | 1990 | 1700692 |
| [40 cases of dengue (serotype 3) occurring in a military camp during an epidemic in new caledonia (1989). the value of vector control]. | an epidemic of dengue occurred at the beginning of 1989 in new caledonia. about 18 p.c. of the population was stricken (25,000 to 30,000 estimated clinical cases). the military camp of plum was stricken too, but a prompt vector control was established. 8.6 p.c. of the strength was affected by classical dengue without severe hemorrhagic manifestations. such prevalence is lower than the one rated in the civil population, demonstrating the major importance of vector control to limit spreading of su ... | 1990 | 2077321 |