Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
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| bacillus cereus from the environment is genetically related to the highly pathogenic b. cereus in zambia. | to follow-up anthrax in zambia since the outbreak in 2011, we have collected samples from the environment and the carcasses of anthrax-suspected animals, and have tried to isolate bacillus anthracis. in the process of identification of b. anthracis, we collected two isolates, of which colonies were similar to b. anthracis; however, from the results of identification using the molecular-based methods, two isolates were genetically related to the highly pathogenic b. cereus, of which clinical mani ... | 2015 | 25797134 |
| the worldwide distribution of genetically and phylogenetically diverse bacillus cereus isolates harbouring bacillus anthracis-like plasmids. | bacillus cereus is a close relative of b. anthracis, the causative agent of anthrax whose pathogenic determinants are located on pxo1 and pxo2 plasmids. bacillus anthracis-like plasmids have been also noted among b. cereus, however, genetic features of b. cereus harbouring these elements remain largely undescribed, especially from the global perspective. herein, we present the genetic polymorphism, population structure and phylogeny of b. cereus with pxo1-/pxo2-like plasmids originating from arg ... | 2015 | 26033739 |
| protein composition of the outermost exosporium-like layer of clostridium difficile 630 spores. | clostridium difficile spores are considered the morphotype of infection, transmission and persistence of c. difficile infections. there is a lack of information on the composition of the outermost exosporium layer of c. difficile spores. using recently developed exosporium removal methods combined with ms/ms, we have established a gel-free approach to analyze the proteome of the exosporium of c. difficile spores of strain 630. a total of 184 proteins were found in the exosporium layer of c. diff ... | 2015 | 25849250 |
| biosynthesis, characterization, and antimicrobial applications of silver nanoparticles. | in the present study, the strain brevibacterium frigoritolerans dc2 was explored for the efficient and extracellular synthesis of silver nanoparticles. these biosynthesized silver nanoparticles were characterized by ultraviolet-visible spectrophotometry, which detected the formation of silver nanoparticles in the reaction mixture and showed a maximum absorbance at 420 nm. in addition, field emission transmission electron microscopy revealed the spherical shape of the nanoparticles. the dynamic l ... | 2015 | 25848272 |
| capsules, toxins and atxa as virulence factors of emerging bacillus cereus biovar anthracis. | emerging b. cereus strains that cause anthrax-like disease have been isolated in cameroon (ca strain) and côte d'ivoire (ci strain). these strains are unusual, because their genomic characterisation shows that they belong to the b. cereus species, although they harbour two plasmids, pbcxo1 and pbcxo2, that are highly similar to the pxo1 and pxo2 plasmids of b. anthracis that encode the toxins and the polyglutamate capsule respectively. the virulence factors implicated in the pathogenicity of the ... | 2015 | 25830379 |
| massive horizontal gene transfer, strictly vertical inheritance and ancient duplications differentially shape the evolution of bacillus cereus enterotoxin operons hbl, cytk and nhe. | bacillus cereus sensu lato comprises eight closely related species including the human pathogens bacillus anthracis and bacillus cereus. within b. cereus sensu lato, chromosomally and plasmid-encoded toxins exist. while plasmid-mediated horizontal gene transfer of the emetic toxin, anthrax and insecticidal toxins is known, evolution of enterotoxin genes within the group has not been studied. | 2015 | 26555390 |
| the bacillus anthracis exosporium: what's the big "hairy" deal? | in some bacillus species, including bacillus subtilis, the coat is the outermost layer of the spore. in others, such as the bacillus cereus family, there is an additional layer that envelops the coat, called the exosporium. in the case of bacillus anthracis, a series of fine hair-like projections, also referred to as a "hairy" nap, extends from the exosporium basal layer. the exact role of the exosporium in b. anthracis, or for any of the bacillus species possessing this structure, remains uncle ... | 2015 | 26542035 |
| evaluation of tools for environmental sampling of bacillus anthracis spores. | this study describes the validation of sampling techniques used to detect biological warfare agents used in terror attacks. for this purpose, we tested the efficiencies of different sampling media and extraction solutions for the recovery of bacterial pathogens. we first used bacillus cereus atcc 4342 spores as a surrogate for highly pathogenic b. anthracis to compare recovery efficiencies of spores from four different surfaces. we used three different types of sampling swabs and four different ... | 2015 | 26528669 |
| the exosporium layer of bacterial spores: a connection to the environment and the infected host. | much of what we know regarding bacterial spore structure and function has been learned from studies of the genetically well-characterized bacterium bacillus subtilis. molecular aspects of spore structure, assembly, and function are well defined. however, certain bacteria produce spores with an outer spore layer, the exosporium, which is not present on b. subtilis spores. our understanding of the composition and biological functions of the exosporium layer is much more limited than that of other ... | 2015 | 26512126 |
| a cluster of cns infections due to b. cereus in the setting of acute myeloid leukemia: neuropathology in 5 patients. | bacillus cereus typically causes a self-limited foodborne gastrointestinal (gi) illness. severe invasive infection occurs rarely, mainly among immunocompromised hosts. we describe a cluster of b. cereus infections among 5 patients with acute myeloid leukemia and chemotherapy-induced neutropenia. the initial case presented with occipital lobe abscess and was found on biopsy to have organisms consistent with bacillus species. within 1 week, a second patient died of fulminant brain swelling and hem ... | 2015 | 26352989 |
| reagent-free and portable detection of bacillus anthracis spores using a microfluidic incubator and smartphone microscope. | bacillus anthracis is the causative agent of anthrax and can be contracted by humans and herbivorous mammals by inhalation, ingestion, or cutaneous exposure to bacterial spores. due to its stability and disease potential, b. anthracis is a recognized biothreat agent and robust detection and viability methods are needed to identify spores from unknown samples. here we report the use of smartphone-based microscopy (spm) in combination with a simple microfluidic incubation device (mid) to detect 50 ... | 2015 | 26266749 |
| identification and characterization of a novel geobacillus thermoglucosidasius bacteriophage, gve3. | the study of extremophilic phages may reveal new phage families as well as different mechanisms of infection, propagation and lysis to those found in phages from temperate environments. we describe a novel siphovirus, gve3, which infects the thermophile geobacillus thermoglucosidasius. the genome size is 141,298 bp (g+c 29.6%), making it the largest geobacillus spp-infecting phage known. gve3 appears to be most closely related to the recently described bacillus anthracis phage vb_bans_tsamsa, ra ... | 2015 | 26123922 |
| inactivation of chemical and heat-resistant spores of bacillus and geobacillus by nitrogen cold atmospheric plasma evokes distinct changes in morphology and integrity of spores. | bacterial spores are resistant to severe conditions and form a challenge to eradicate from food or food packaging material. cold atmospheric plasma (cap) treatment is receiving more attention as potential sterilization method at relatively mild conditions but the exact mechanism of inactivation is still not fully understood. in this study, the biocidal effect by nitrogen cap was determined for chemical (hypochlorite and hydrogen peroxide), physical (uv) and heat-resistant spores. the three diffe ... | 2015 | 25481059 |
| infective endocarditis due to bacillus cereus in a pregnant female: a case report and literature review. | incidence of infective endocarditis during pregnancy is around 0.006% with high maternal and fetal mortality. bacillus cereus is an extremely rare cause for endocarditis in intravenous drug abusers (ivda) or those with valvular disease or devices such as pacemakers. we report a case of b. cereus endocarditis, which, to the best of our knowledge, has never been reported in pregnancy. a 30-year-old, 25-week pregnant female presented with right shoulder pain, swelling and erythema on the lateral as ... | 2015 | 26793477 |
| starch based biodegradable graft copolymer for the preparation of silver nanoparticles. | the synthesis and characterization of a novel biodegradable graft copolymer based on partially hydrolyzed polymethylacrylate (pma) grafted amylopectin (ap) was reported which was developed for the synthesis of silver nanoparticles from silver nitrate solution by facile green technique. the prepared graft copolymer was biodegradable which was shown by fungal growth. characterization of silver nanoparticles was carried out by uv-vis spectroscopy (417nm), hr-tem, saed and fesem analysis. the tem fi ... | 2015 | 26231330 |
| fatty acid profiles and desaturase-encoding genes are different in thermo- and psychrotolerant strains of the bacillus cereus group. | the bacillus cereus group consists of closely-related bacteria, including pathogenic or harmless strains, and whose species can be positioned along the seven phylogenetic groups of guinebretière et al. (i-vii). they exhibit different growth-temperature ranges, through thermotolerant to psychrotolerant thermotypes. among these, b. cytotoxicus is an atypical thermotolerant and food-poisoning agent affiliated to group vii whose thermotolerance contrasts with the mesophilic and psychrotolerant therm ... | 2015 | 26227277 |
| amplicon sequencing for the quantification of spoilage microbiota in complex foods including bacterial spores. | spoilage of food products is frequently caused by bacterial spores and lactic acid bacteria. identification of these organisms by classic cultivation methods is limited by their ability to form colonies on nutrient agar plates. in this study, we adapted and optimized 16s rrna amplicon sequencing for quantification of bacterial spores in a canned food matrix and for monitoring the outgrowth of spoilage microbiota in a ready-to-eat food matrix. | 2015 | 26217487 |
| lithocholic acid and derivatives: antibacterial activity. | in order to develop bioactive lithocholic acid derivatives, we prepared fifteen semi-synthetic compounds through modification at c-3 and/or c-24. the reactions showed yields ranging from 37% to 100%. the structures of all compounds obtained were identified on the basis of their spectral data (ir, ms, 1d- and 2d-nmr). the activity of lithocholic acid and derivatives was evaluated against the growth of escherichia coli, staphylococcus aureus, bacillus cereus and pseudomonas aeruginosa. the derivat ... | 2015 | 26216208 |
| chitinolytic bacteria-assisted conversion of squid pen and its effect on dyes and pigments adsorption. | the aim of this work was to produce chitosanase by fermenting from squid pen, and recover the fermented squid pen for dye removal by adsorption. one chitosanase induced from squid pen powder (spp)-containing medium by bacillus cereus tku034 was purified in high purification fold (441) and high yield of activity recovery (51%) by ammonium sulfate precipitation and combined column chromatography. the sds-page results showed its molecular mass to be around 43 kda. the tku034 chitosanase used for th ... | 2015 | 26213948 |
| sol-gel encapsulation of binary zn(ii) compounds in silica nanoparticles. structure-activity correlations in hybrid materials targeting zn(ii) antibacterial use. | in the emerging issue of enhanced multi-resistant properties in infectious pathogens, new nanomaterials with optimally efficient antibacterial activity and lower toxicity than other species attract considerable research interest. in an effort to develop such efficient antibacterials, we a) synthesized acid-catalyzed silica-gel matrices, b) evaluated the suitability of these matrices as potential carrier materials for controlled release of znso4 and a new zn(ii) binary complex with a suitably des ... | 2015 | 26198972 |
| chemical composition of nardostachys grandiflora rhizome oil from nepal--a contribution to the chemotaxonomy and bioactivity of nardostachys. | the essential oil from the dried rhizome of nardostachys grandiflora, collected from jaljale, nepal, was obtained in 1.4% yield, and a total of 72 compounds were identified constituting 93.8% of the essential oil. the rhizome essential oil of n. grandiflora was mostly composed of calarene (9.4%), valerena-4,7(11)-diene (7.1%), nardol a (6.0%), 1(10)-aristolen-9β-ol (11.6%), jatamansone (7.9%), valeranal (5.6%), and cis-valerinic acid (5.7%). the chemical composition of n. grandiflora rhizome oil ... | 2015 | 26197553 |
| unexpected roles for toll-like receptor 4 and trif in intraocular infection with gram-positive bacteria. | inflammation caused by infection with gram-positive bacteria is typically initiated by interactions with toll-like receptor 2 (tlr2). endophthalmitis, an infection and inflammation of the posterior segment of the eye, can lead to vision loss when initiated by a virulent microbial pathogen. endophthalmitis caused by bacillus cereus develops as acute inflammation with infiltrating neutrophils, and vision loss is potentially catastrophic. residual inflammation observed during b. cereus endophthalmi ... | 2015 | 26195555 |
| is cytotoxin k from bacillus cereus a bona fide enterotoxin? | cytotoxin k (cytk) produced by bacillus cereus s.l. has generally been considered to be associated with the foodborne diarrhoeal syndrome. two distinct variants of cytk have been reported: cytk-1 from bacillus cytotoxicus and cytk-2 from b. cereus. in order to determine whether cytk plays a significant role in the diarrhoeal disease, the occurrence of cytk genes was assessed among 390 b. cereus isolates with different origins including clinical and food poisoning samples and was found to be 46%. ... | 2015 | 26186121 |
| food-bacteria interplay: pathometabolism of emetic bacillus cereus. | bacillus cereus is a gram-positive endospore forming bacterium known for its wide spectrum of phenotypic traits, enabling it to occupy diverse ecological niches. although the population structure of b. cereus is highly dynamic and rather panmictic, production of the emetic b. cereus toxin cereulide is restricted to strains with specific genotypic traits, associated with distinct environmental habitats. cereulide is an ionophoric dodecadepsipeptide that is produced non-ribosomally by an enzyme co ... | 2015 | 26236290 |
| complete genome sequence analysis and identification of putative metallo-beta-lactamase and spoiiie homologs in bacillus cereus group phage bcp8-2, a new member of the proposed bastille-like group. | bacillus cereus group-specific bacteriophage bcp8-2 exhibits a broad lysis spectrum among food and human isolates (330/364) of b. cereus while not infecting b. subtilis (50) or b. licheniformis (12) strains. its genome is 159,071 bp long with 220 open reading frames, including genes for putative methyltransferases, metallo-beta-lactamase, and a sporulation-related spoiiie homolog, as wells as 18 trnas. comparative genome analysis showed that bcp8-2 is related to the recently proposed bastille-li ... | 2015 | 26234184 |
| endophthalmitis caused by bacillus cereus: a devastating ophthalmological emergency. | 2015 | 26493084 | |
| quantitative prevalence and toxin gene profile of bacillus cereus from ready-to-eat vegetables in south korea. | ready-to-eat (rte) foods such as prepared vegetables are becoming an increasingly popular food choice. since rte vegetables are not commonly sterilized by heat treatment, contamination with foodborne pathogens such as bacillus cereus (b. cereus) is a major concern. the objective of this study was to assess the quantitative prevalence and toxin gene profiles of b. cereus strains isolated from rte vegetables. we found that 70 of the 145 (48%) tested retail vegetable salad and sprout samples were p ... | 2015 | 26317539 |
| food sensing: aptamer-based trapping of bacillus cereus spores with specific detection via real time pcr in milk. | aerobic spores pose serious problems for both food product manufacturers and consumers. milk is particularly at risk and thus an important issue of preventive consumer protection and quality assurance. the spore-former bacillus cereus is a food poisoning gram-positive pathogen which mainly produces two different types of toxins, the diarrhea inducing and the emetic toxins. reliable and rapid analytical assays for the detection of b. cereus spores are required, which could be achieved by combinin ... | 2015 | 26306797 |
| analysis of the bacillus cereus spoiis antitoxin-toxin system reveals its three-component nature. | programmed cell death in bacteria is generally associated with two-component toxin-antitoxin systems. the spoiis toxin-antitoxin system, consisting of a membrane-bound spoiisa toxin and a small, cytosolic antitoxin spoiisb, was originally identified in bacillus subtilis. in this work we describe the bacillus cereus spoiis system which is a three-component system, harboring an additional gene spoiisc. its protein product serves as an antitoxin, and similarly as spoiisb, is able to bind spoiisa an ... | 2015 | 26300872 |
| inhibition of quorum sensing-mediated biofilm formation in pseudomonas aeruginosa by a locally isolated bacillus cereus. | quorum sensing has been shown to play a crucial role in pseudomonas aeruginosa pathogenesis where it activates expression of myriad genes that regulate the production of important virulence factors such as biofilm formation. antagonism of quorum sensing is an excellent target for antimicrobial therapy and represents a novel approach to combat drug resistance. in this study, chromobacterium violaceum biosensor strain was employed as a fast, sensitive, reliable, and easy to use tool for rapid scre ... | 2015 | 26288125 |
| toxin profile, biofilm formation, and molecular characterization of emetic toxin-producing bacillus cereus group isolates from human stools. | emetic toxin-producing bacillus cereus group species are an important problem, because the staple food for korean is grains such as rice. in this study, we determined the prevalence (24 of 129 isolates) of emetic b. cereus in 36,745 stool samples from sporadic food-poisoning cases in korea between 2007 and 2008. the toxin gene profile, toxin production, and biofilm-forming ability of the emetic b. cereus isolates were investigated. repetitive element sequence polymorphism polymerase chain reacti ... | 2015 | 26287636 |
| antibacterial effects and action modes of asiatic acid. | in this study, the antibacterial effects and action modes of asiatic acid against the foodborne bacterial pathogens escherichia coli o157:h7, salmonella typhimurium dt104, pseudomonas aeruginosa, listeria monocytogenes, staphylococcus aureus, enterococcus faecalis, and bacillus cereus were examined. minimal inhibitory concentrations (mics) of asiatic acid against these bacteria were in the range of 20-40 μg/ml. minimum bactericidal concentrations of asiatic acid were in the range of 32-52 μg/ml. ... | 2015 | 26280399 |
| comparison of oxytetracycline degradation behavior in pig manure with different antibiotic addition methods. | using manure collected from swine fed with diet containing antibiotics and antibiotic-free swine manure spiked with antibiotics are the two common methods of studying the degradation behavior of veterinary antibiotic in manure in the environment. however, few studies had been conducted to co-compare these two different antibiotic addition methods. this study used oxytetracycline (otc) as a model antibiotic to study antibiotic degradation behavior in manure under the above two otc addition method ... | 2015 | 26278905 |
| bacillus cereus cerebral abscess during induction chemotherapy for childhood acute leukemia. | a 5-year-old boy with standard-risk b-cell acute lymphoblastic anemia developed fever during induction chemotherapy. the patient had no neurological symptoms. blood cultures grew bacillus cereus and neuroimaging studies demonstrated a cerebral abscess. imaging changes resolved after completion of antibiotics. bacillus cereus bacteremia is increasingly implicated as the cause of life-threatening infections, including cerebral abscesses, in compromised patients. positive blood cultures for this or ... | 2015 | 26274034 |
| a type iii protein-rna toxin-antitoxin system from bacillus thuringiensis promotes plasmid retention during spore development. | members of the bacillus cereus sensu lato group of bacteria often contain multiple large plasmids, including those encoding virulence factors in b. anthracis. bacillus species can develop into spores in response to stress. during sporulation the genomic content of the cell is heavily compressed, which could result in counterselection of extrachromosomal genomic elements, unless they have robust stabilization and segregation systems. toxin-antitoxin (ta) systems are near-ubiquitous in prokaryotes ... | 2015 | 26274022 |
| isolation and characterisation of non-anaerobic butanol-producing symbiotic system tsh06. | butanol-producing microorganisms are all obligate anaerobes. in this study, a unique symbiotic system tsh06 was isolated to be capable of producing butanol under non-anaerobic condition. denaturing gradient gel electrophoresis (dgge) analysis of 16s ribosomal rna (rrna) revealed that two strains coexist in tsh06. the two strains were identical to clostridium acetobutylicum and bacillus cereus, respectively. they were isolated individually and named as c. acetobutylicum tsh1 and b. cereus tsh2. c ... | 2015 | 26272091 |
| epidemiologic investigation of a cluster of neuroinvasive bacillus cereus infections in 5 patients with acute myelogenous leukemia. | background. five neuroinvasive bacillus cereus infections (4 fatal) occurred in hospitalized patients with acute myelogenous leukemia (aml) during a 9-month period, prompting an investigation by infection control and public health officials. methods. medical records of case-patients were reviewed and a matched case-control study was performed. infection control practices were observed. multiple environmental, food, and medication samples common to aml patients were cultured. multilocus sequenc ... | 2015 | 26269794 |
| production and characterization of poly-3-hydroxybutyrate from bacillus cereus ps 10. | usage of renewable raw materials for production of fully degradable bioplastics (bacterial poly-3-hydroxybutyrate, phb) has gained immense research impetus considering recalcitrant nature of petroleum based plastics, dwindling fossil fuel feed stocks, and associated green house gas emissions. however, high production cost of phb is the major bottleneck for its wide range industrial applications. in current study, bacillus cereus ps 10, a recent isolate, efficiently utilized molasses, an abundant ... | 2015 | 26257381 |
| a novel enzyme-based antimicrobial system comprising iodide and a multicopper oxidase isolated from alphaproteobacterium strain q-1. | alphaproteobacterium strain q-1 produces an extracellular multicopper oxidase (iox), which catalyzes iodide (i-) oxidation to form molecular iodine (i2). in this study, the antimicrobial activity of the iox/iodide system was determined. both gram-positive and gram-negative bacteria tested were killed completely within 5 min by 50 mu ml(-1) of iox and 10 mm iodide. the sporicidal activity of the system was also tested and compared with a common iodophor, povidone-iodine (pvp-i). iox (300 mu ml(-1 ... | 2015 | 26254787 |
| genome sequence of bacillus endophyticus and analysis of its companion mechanism in the ketogulonigenium vulgare-bacillus strain consortium. | bacillus strains have been widely used as the companion strain of ketogulonigenium vulgare in the process of vitamin c fermentation. different bacillus strains generate different effects on the growth of k. vulgare and ultimately influence the productivity. first, we identified that bacillus endophyticus hbe603 was an appropriate strain to cooperate with k. vulgare and the product conversion rate exceeded 90% in industrial vitamin c fermentation. here, we report the genome sequencing of the b. e ... | 2015 | 26248285 |
| multiparametric quantitation of the bacillus cereus toxins cereulide and isocereulides a-g in foods. | consumption of food products contaminated with cereulide (1), a toxin produced by bacillus cereus, might cause intoxications with symptoms reported to range from indigestion pain and emesis to death. recently, a series of structural variants, coined isocereulides a-g (2-8), were identified for the first time to be produced along with cereulide (1). the observation that isocereulide a (2) shows an ∼ 8-fold increased cytotoxicity when compared to 1 urges the development of analytical tools enablin ... | 2015 | 26352912 |
| chemical composition and in vitro antibacterial activity of mentha spicata essential oil against common food-borne pathogenic bacteria. | the aim of the present study was to investigate chemical composition and antibacterial activity of essential oil from the leaf of mentha spicata plant against common food-borne pathogenic bacteria (staphylococcus aureus, bacillus subtilis, bacillus cereus, listeria monocytogenes, salmonella typhimurium, and escherichia coli o157:h7). chemical composition of the essential oil was identified by gas chromatography coupled with mass spectrometer detector (gc-ms). the antibacterial activity of the es ... | 2015 | 26351584 |
| reduction of listeria monocytogenes and bacillus cereus in milk by zinc oxide nanoparticles. | direct addition of antimicrobial materials to food during food processing is an effective method for controlling microbial contaminants of food and extending the shelf- life of food products. objective of this research was to study the antimicrobial effect of zinc oxide (zno) nanoparticle and potential applications of zno nanoparticles in terms of controling two food-borne pathogens in milk. | 2015 | 26351469 |
| a study on characterization of new bacteriocin produced from a novel strain of lactobacillus spicheri g2 isolated from gundruk- a fermented vegetable product of north east india: a novel bacteriocin production from lactobacillus spicheri g2. | bacteriocin producing lactobacillus spicheri g2, isolated from gundruk - a traditional fermented vegetable product of north east india. l. spicheri g2 identified by morphological, biochemical techniques followed by 16s rrna gene technique. the 16sr rna sequence of bacteriocin producer is registered in ncbi under accession no. jx481912. the bacteriocin producing potential of l. spicheri is being reported for the first time in the present investigation. bacteriocin of l. spicheri g2 showed strong ... | 2015 | 26344995 |
| poly(n-isopropylacrylamide)-gated fe3o4/sio2 core shell nanoparticles with expanded mesoporous structures for the temperature triggered release of lysozyme. | core-shell nanoparticles comprised of fe3o4 cores and a mesoporous silica shell with an average expanded pore size of 6.07 nm and coated with a poly(n-isopropylacrylamide) (pnipam) layer (cs-msns-ep-pnipam) were prepared and characterized. the nanoparticles was loaded with (ru(bipy)3(2+)) dye or an antibacterial enzyme, lysozyme, to obtain cs-msns-ep-pnipam-ru(bipy)3(2+) and cs-msns-ep-pnipam-lys, respectively. the lysozyme loading was determined to be 160 mg/g of nanoparticle. it was seen that ... | 2015 | 26335056 |
| structural and computational analysis of peptide recognition mechanism of class-c type penicillin binding protein, alkaline d-peptidase from bacillus cereus df4-b. | alkaline d-peptidase from bacillus cereus df4-b, called adp, is a d-stereospecific endopeptidase reacting with oligopeptides containing d-phenylalanine (d-phe) at n-terminal penultimate residue. adp has attracted increasing attention because it is useful as a catalyst for synthesis of d-phe oligopeptides or, with the help of substrate mimetics, l-amino acid peptides and proteins. structure and functional analysis of adp is expected to elucidate molecular mechanism of adp. in this study, the crys ... | 2015 | 26370172 |
| clinical characteristics and antimicrobial susceptibility of bacillus cereus blood stream infections. | bacillus cereus is one of the pathogens causing nosocomial bloodstream infections (bsis). however, few reports have documented the antimicrobial susceptibility and clinical characteristics of bacillus cereus bsi and the importance of empirical therapy. the aim of this study was to investigate the clinical characteristics and antimicrobial susceptibility of b. cereus isolates from patients with bsi and to analyze the impact of appropriate empirical therapy on the outcome of patients with b. cereu ... | 2015 | 26370137 |
| high-rate biological denitrification in the cyclic rotating-bed biological reactor: effect of cod/no3(-), nitrate concentration and salinity and the phylogenetic analysis of denitrifiers. | the effects of cod/no3(-) ratio, nitrate concentration and salinity was tested on the performance of the crbr in denitrification with catechol as carbon source. the maximum nitrate reduction attained at cod/no3(-) ratio of 1. the crbr operated at optimum cod/no3(-) ratio could completely denitrify the nitrate at inlet concentration up to 1250mg/l without nitrite accumulation. the maximum denitrification rate in the crbr was 3.56kgno3(-)/m(3)d with a nitrate reduction efficiency of 99% when the b ... | 2015 | 26369277 |
| merosesquiterpenoids and ten-membered macrolides from a soft coral-derived lophiostoma sp. fungus. | one new merosesquiterpenoid, craterellin d (1), along with one known analog, craterellin a (2), and five known ten-membered macrolides, 3-7, were isolated from a soft coral-derived lophiostoma sp. fungus. the absolute configuration of 1 was established based on biogenetic consideration with the co-isolated analog 2, whose configuration was determined by modified mosher's method and single-crystal x-ray diffraction analysis using cukα radiation. the absolute configuration of 3 was determined by x ... | 2015 | 26363884 |
| essential oils and diethyl ether extracts of serbian xeranthemum cylindraceum and x. annum: chemical composition, antimicrobial activity, and chemotaxonomic implications. | detailed gc and gc-ms analyses of the essential oils and et2 o extracts of two xeranthemum species - x. cylindraceum and x. annum - resulted in the identification of 254 components, in total. terpenoids constituted the major part of both x. cylindraceum and x. annum essential oils and extracts (51.8-65.7%, and 50.7%, resp.). among the sesquiterpenoids, the extracts of both investigated taxa contained the guaianolide xerantholide, its 11,13-dihydro derivatives, and two additional sesquiterpene la ... | 2015 | 26363882 |
| identification of novel nitroreductases from bacillus cereus and their interaction with the cb1954 prodrug. | directed enzyme prodrug therapy is a form of cancer chemotherapy in which bacterial prodrug-activating enzymes, or their encoding genes, are directed to the tumour before administration of a prodrug. the prodrug can then be activated into a toxic drug at the tumour site, reducing off-target effects. the bacterial nitroreductases are a class of enzymes used in this therapeutic approach and although very promising, the low turnover rate of prodrug by the most studied nitroreductase enzyme, nfnb fr ... | 2015 | 26415543 |
| identification and characterization of bacillus cereus sw7-1 in bombyx mori (lepidoptera: bombycidae). | the bacterial diseases of silkworms cause significant reductions in sericulture and result in huge economic loss. this study aimed to identify and characterize a pathogen from diseased silkworm. sw7-1, a pathogenic bacterial strain, was isolated from the diseased silkworm. the strain was identified on the basis of its bacteriological properties and 16s rrna gene sequence. the colony was round, slightly convex, opaque, dry, and milky on a nutrient agar medium, the colony also exhibited jagged edg ... | 2015 | 26411789 |
| composition, in vitro cytotoxic, and antimicrobial activities of the flower essential oil of diospyros discolor from taiwan. | this study investigated the chemical composition, in vitro cytotoxic, and antimicrobial activities of the essential oil isolated from the flower of diospyros discolor from taiwan. the essential oil was isolated using hydrodistillation in a clevenger-type apparatus, and characterized by gc-fid and gc-ms. twenty-eight compounds were identified, representing 100% of the oil. the main components identified were (2z,6e)-farnesol (35.0%), α-cadinol (10.9%), (e)-nerolidol (6.9%), α-humulene (6.0%), τ-c ... | 2015 | 26411038 |
| isolation and characterization of bacteriocinogenic lactic bacteria from m-tuba and tepache, two traditional fermented beverages in méxico. | mexican tuba (m-tuba) and tepache are mexican fermented beverages prepared mainly with pineapple pulp and coconut palm, respectively. at present, reports on the microbiota and nutritional effects of both beverages are lacking. the purpose of this study was to determine whether m-tuba and tepache contain cultivable lactic acid bacteria (lab) capable of producing bacteriocins. tepache and m-tuba contain mesophilic aerobic bacteria, lab, and yeast. bacillus subtilis, listeria monocytogenes, listeri ... | 2015 | 26405529 |
| antibacterial effect and mechanism of high-intensity 405 ± 5 nm light emitting diode on bacillus cereus, listeria monocytogenes, and staphylococcus aureus under refrigerated condition. | this study investigated the antibacterial effect of 405 ± 5 nm light emitting diode (led) on bacillus cereus, listeria monocytogenes, and staphylococcus aureus, and examined its antibacterial mechanism by determining the bacterial membrane and dna damages. a 405 ± 5 nm led illuminated the gram-positive pathogens until 486 j/cm(2) at 4 °c. weibull model was used to calculate reliable life (tr) to compare bacterial sensitivities to led illumination. the membrane damage was determined by nacl and l ... | 2015 | 26398810 |
| health-promoting value and food applications of black cumin essential oil: an overview. | black cumin (nigella sativa l.) seeds and its essential oil have been widely used in functional foods, nutraceuticals and pharmaceutical products. analysis of nigella sativa essential oil using gc and gc-ms resulted in the identification of many bioactive compounds representing ca. 85 % of the total content. the main compounds included p-cymene, thymoquinone, α-thujene, longifolene, β-pinene, α-pinene and carvacrol. nigella sativa essential oil exhibited different biological activities including ... | 2015 | 26396361 |
| complete genome sequence of bacillus thuringiensis strain hd521. | bacillus thuringiensis is the most widely used biological pesticide in the world. it belongs to the bacillus cereus sensu lato group, which contains six species. among these six species, b. thuringiensis, b. anthracis, and b. cereus have a low genetic diversity. b. thuringiensis strain hd521 shows maroon colony which is different from most of the b. thuringiensis strains. strain hd521 also displays an ability to inhibit plant sheath blight disease pathogen (rhizoctonia solani ag1 ib) growth and ... | 2015 | 26380647 |
| genomic insights into the taxonomic status of the bacillus cereus group. | the identification and phylogenetic relationships of bacteria within the bacillus cereus group are controversial. this study aimed at determining the taxonomic affiliations of these strains using the whole-genome sequence-based genome blast distance phylogeny (gbdp) approach. the gbdp analysis clearly separated 224 strains into 30 clusters, representing eleven known, partially merged species and accordingly 19-20 putative novel species. additionally, 16s rrna gene analysis, a novel variant of mu ... | 2015 | 26373441 |
| [noma/cancrum oris: a neglected disease]. | noma is an aggressive orofacial gangrenous pathology that damages hard and soft tissues of the mouth and the face. throughout the centuries it has been present around the globe, but nowadays it has practically disappeared from developed countries and mainly affects children from the most disadvantaged places, especially in africa. noma disease is a multifactorial process; malnutrition, debilitating diseases (bacterial or viral systemic diseases, hiv-associated immunosuppression, etc.) and intrao ... | 2015 | 26437752 |
| short communication: elucidation of bacterial community structure on thin-spined porcupine (chaetomys subspinosus) spines by denaturing. | thin-spined porcupines (chaetomys subspinosus) are threatened with extinction and are categorized as vulnerable. this is because of alteration to and loss of their habitat and possible hunting activities in their distribution area. their spines constitute one of their defense mechanisms, which can be fomites for pathogens to humans. however, little is known about such pathogens. the present study aimed to detect bacteria on spines of c. subspinosus, from the una biological reserve, south of bahi ... | 2015 | 26436511 |
| differential oxidative stress responses in castor semilooper, achaea janata. | balance between reactive oxygen species (ros) and the antioxidant (ao) defense mechanisms is vital for organism survival. insects serve as an ideal model to elucidate oxidative stress responses as they are prone to different kinds of stress during their life cycle. the present study demonstrates the modulation of ao enzyme gene expression in the insect pest, achaea janata (castor semilooper), when subjected to different oxidative stress stimuli. antioxidant enzymes' (catalase (cat), superoxide d ... | 2015 | 26455997 |
| novel diesel-oil-degrading bacteria and fungi from the ecuadorian amazon rainforest. | isolating new diesel-oil-degrading microorganisms from crude-oil contaminated sites and evaluating their degradation capacities are vitally important in the remediation of oil-polluted environments and crude-oil exploitation. in this research, new hydrocarbon-degrading bacteria and fungi were isolated from the crude-oil contaminated soil of the oil-fields in the amazon rainforest of north-east ecuador by using a soil enrichment technique. degradation analysis was tracked by gas chromatography an ... | 2015 | 26442498 |
| evaluation of bacterial contamination in raw milk, ultra-high temperature milk and infant formula using single molecule, real-time sequencing technology. | the pacific biosciences (menlo park, ca) single molecule, real-time sequencing technology (smrt) was reported to have some advantages in analyzing the bacterial profile of environmental samples. in this study, the presence of bacterial contaminants in raw milk, uht milk, and infant formula was determined by smrt sequencing of the full length 16s rrna gene. the bacterial profiles obtained at different taxonomic levels revealed clear differences in bacterial community structure across the 16 analy ... | 2015 | 26476945 |
| synthesis, evaluation and molecular docking studies of amino acid derived n-glycoconjugates as antibacterial agents. | six amino acid derived n-glycoconjugates of d-glucose were synthesized, characterized and tested for antibacterial activity against g(+)ve (bacillus cereus) as well as g(-)ve (escherichia coli and klebsiella pneumoniae) bacterial strains. all the tested compounds exhibited moderate to good antibacterial activity against these bacterial strains. the results were compared with the antibacterial activity of standard drug chloramphenicol, where results of a5 (tryptophan derived glycoconjugates) agai ... | 2015 | 26476390 |
| purification and characterization of bacteriocin produced by weissella confusa a3 of dairy origin. | a dramatic increase in bacterial resistance towards currently available antibiotics has raised worldwide concerns for public health. therefore, antimicrobial peptides (amps) have emerged as a promisingly new group of therapeutic agents for managing infectious diseases. the present investigation focusses on the isolation and purification of a novel bacteriocin from an indigenous sample of cow milk and it's mode of action. the bacteriocin was isolated from weissella confusa a3 that was isolated fr ... | 2015 | 26474074 |
| enteric bacteria isolated from diarrheal patients in korea in 2014. | the aim of this study was to characterize the pathogens responsible for causing diarrhea according to season, region of isolation, patient age, and sex as well as to provide useful data for the prevention of diarrheal disease. | 2015 | 26473090 |
| genome sequences of two bacillus cereus group bacteriophages, eyuki and avesobmore. | the genomes of two double-stranded dna (dsdna) bacteriophages isolated on bacillus thuringiensis show similarity to previously sequenced phages and provide evidence of the mosaicism of phage genomes. | 2015 | 26472840 |
| complete genome sequence of bacillus cereus group phage tsarbomba. | the bacillus cereus group bacteriophage tsarbomba, a double-stranded dna myoviridae, was isolated from soil collected in saratov, russia. tsarbomba was found to be similar to bacillus phages bcp78 and bcu4, and to have a wide host range among bacillus cereus group species. | 2015 | 26472830 |
| total phenolic and flavonoid content and antibacterial activity of punica granatum l. var. pleniflora flowers (golnar) against bacterial strains causing foodborne diseases. | flowers of punica granatum l. (punicaceae) var. pleniflora, known as "golnar" in iranian traditional medicine have been used for the prevention and treatment of foodborne diseases. in this study, antibacterial activities of ethanol extract of golnar and its fractions were scientifically evaluated against bacteria causing foodborne diseases including staphylococcus aureus, bacillus cereus, listeria monocytogenes, escherichia coli, shigella dysantriae, and salmonella typhi. the total phenolic and ... | 2015 | 26470879 |
| proteomics identifies bacillus cereus entd as a pivotal protein for the production of numerous virulence factors. | bacillus cereus is a gram-positive pathogen that causes a wide variety of diseases in humans. it secretes into the extracellular milieu proteins that may contribute directly or indirectly to its virulence. entd is a novel exoprotein identified by proteogenomics of b. cereus atcc 14579. we constructed a δentd mutant and analyzed the impact of entd disruption on the cellular proteome and exoproteome isolated from early, late, and stationary-phase cultures. we identified 308 and 79 proteins regulat ... | 2015 | 26500610 |
| antioxidant, antibacterial activity, and phytochemical characterization of melaleuca cajuputi extract. | the threat posed by drug-resistant pathogens has resulted in the increasing momentum in research and development for effective alternative medications. the antioxidant and antibacterial properties of phytochemical extracts makes them attractive alternative complementary medicines. therefore, this study evaluated the phytochemical constituents of melaleuca cajuputi flower and leaf (gf and gl, respectively) extracts and their antioxidant and antibacterial activities. | 2015 | 26497742 |
| bacillus cereus nvh 0500/00 can adhere to mucin but cannot produce enterotoxins during gastrointestinal simulation. | adhesion to the intestinal epithelium could constitute an essential mechanism of bacillus cereus pathogenesis. however, the enterocytes are protected by mucus, a secretion composed mainly of mucin glycoproteins. these may serve as nutrients and sites of adhesion for intestinal bacteria. in this study, the food poisoning bacterium b. cereus nvh 0500/00 was exposed in vitro to gastrointestinal hurdles prior to evaluation of its attachment to mucin microcosms and its ability to produce nonhemolytic ... | 2015 | 26497468 |
| sporulation temperature reveals a requirement for cote in the assembly of both the coat and exosporium layers of bacillus cereus spores. | the bacillus cereus spore surface layers consist of a coat surrounded by an exosporium. we investigated the interplay between the sporulation temperature and the cote morphogenetic protein in the assembly of the surface layers of b. cereus atcc 14579 spores and on the resulting spore properties. the cote deletion affects the coat and exosporium composition of the spores formed both at the suboptimal temperature of 20°c and at the optimal growth temperature of 37°c. transmission electron microsco ... | 2015 | 26497467 |
| the c-terminal zwitterionic sequence of cotb1 is essential for biosilicification of the bacillus cereus spore coat. | silica is deposited in and around the spore coat layer of bacillus cereus, and enhances the spore's acid resistance. several peptides and proteins, including diatom silaffin and silacidin peptides, are involved in eukaryotic silica biomineralization (biosilicification). homologous sequence search revealed a silacidin-like sequence in the c-terminal region of cotb1, a spore coat protein of b. cereus. the negatively charged silacidin-like sequence is followed by a positively charged arginine-rich ... | 2015 | 26503850 |
| microbiological quality of raw dried pasta from the german market, with special emphasis on cronobacter species. | the microbiological quality of 132 dried pasta products available on the german market, originating from 11 different countries, was studied. sample materials included soft or durum wheat products, some of which produced with other ingredients such as eggs, spices, or vegetables. parameters included hygiene indicators (aerobic plate count, mold count, the presence of enterobacteriaceae) and pathogenic/toxinogenic bacterial species (salmonella spp., staphylococcus aureus, presumptive bacillus cer ... | 2015 | 26509868 |
| the dna glycosylase alkd uses a non-base-flipping mechanism to excise bulky lesions. | threats to genomic integrity arising from dna damage are mitigated by dna glycosylases, which initiate the base excision repair pathway by locating and excising aberrant nucleobases. how these enzymes find small modifications within the genome is a current area of intensive research. a hallmark of these and other dna repair enzymes is their use of base flipping to sequester modified nucleotides from the dna helix and into an active site pocket. consequently, base flipping is generally regarded a ... | 2015 | 26524531 |
| high-level production of bacillus cereus phospholipase c in corynebacterium glutamicum. | enzymatic oil degumming (removal of phospholipids) using phospholipase c (plc) is a well-established and environmentally friendly process for vegetable oil refining. in this work, we report the production of recombinant bacillus cereus plc in corynebacterium glutamicum atcc 13869 in a high cell density fermentation process and its performance in soybean oil degumming. a final concentration of 5.5g/l of the recombinant enzyme was achieved when the respective gene was expressed from the tac promot ... | 2015 | 26519562 |
| next-generation whole-genome sequencing of eight strains of bacillus cereus, isolated from food. | bacillus cereus can contaminate food and cause emetic and diarrheal foodborne illness. here, we report whole-genome sequences of eight strains of b. cereus, isolated from different food sources. | 2015 | 26679589 |
| subset selection of high-depth next generation sequencing reads for de novo genome assembly using mapreduce framework. | recent progress in next-generation sequencing technology has afforded several improvements such as ultra-high throughput at low cost, very high read quality, and substantially increased sequencing depth. state-of-the-art high-throughput sequencers, such as the illumina miseq system, can generate ~15 gbp sequencing data per run, with >80% bases above q30 and a sequencing depth of up to several 1000x for small genomes. illumina hiseq 2500 is capable of generating up to 1 tbp per run, with >80% bas ... | 2015 | 26678408 |
| erratum for erill and caruso, complete genome sequence of bacillus cereus group phage tsarbomba. | 2015 | 26586903 | |
| fibrinolytic enzyme production by newly isolated bacillus cereus srm-001 with enhanced in-vitro blood clot lysis potential. | the discovery of plasmin-like microbial fibrinolytic enzymes having high specificity and negligible side effects is crucial for thrombolytic therapy. herein, we report one such extra-cellular fibrinolytic enzyme producing bacillus cereus srm-001 isolated from the blood-laden soil of a chicken dump yard. the potency of the enzyme was established with fibrin plate assay and in-vitro blood clot lysis assay. the shake-flask operating parameters and media composition were optimized for maximizing the ... | 2015 | 26582284 |
| phenalenone derivatives and the unusual tricyclic sesterterpene acid from the marine fungus lophiostoma bipolare bcc25910. | ten compounds including nine phenalenone derivatives (five bipolarides and four bipolarols) and a sesterterpene acid (bipolarenic acid), were isolated from a marine isolated of the fungus lophiostoma bipolare (bcc25910), along with the known compounds, (-)-scleroderolide, (-)-sclerodin, and oxasetin. chemical structures were elucidated based on nmr spectroscopic data and hresims analysis. in addition, the absolute configurations of the phenalenones were resolved using specific rotations and chem ... | 2015 | 26582262 |
| the mutation glu151asp in the b-component of the bacillus cereus non-hemolytic enterotoxin (nhe) leads to a diverging reactivity in antibody-based detection systems. | the ability of bacillus cereus to cause foodborne toxicoinfections leads to increasing concerns regarding consumer protection. for the diarrhea-associated enterotoxins, the assessment of the non-hemolytic enterotoxin b (nheb) titer determined by a sandwich enzyme immunoassay (eia) correlates best with in vitro cytotoxicity. in general, the regulation of enterotoxin expression of b. cereus is a coordinately-regulated process influenced by environmental, and probably also by host factors. as long ... | 2015 | 26569304 |
| design and synthesis of new 2-aryl-4,5-dihydro-thiazole analogues: in vitro antibacterial activities and preliminary mechanism of action. | sixty 2-aryl-4,5-dihydrothiazoles were designed and synthesized in yields ranging from 64% to 89% from cysteine and substituted-benzonitriles via a novel metal- and catalyst-free method. the structures of the title compounds were confirmed mainly by nmr spectral data analysis. antibacterial activity assays showed that the compounds (s)-2-(2'-hydroxyphenyl)-4-hydroxy-methyl- 4,5-dihydrothiazole (7h) and (r)-2-(2'-hydroxyphenyl)-4-hydroxymethyl-4,5-dihydro-thiazole (7h') exhibited significant inhi ... | 2015 | 26569197 |
| complete genome sequence of bacillus cereus forc_005, a food-borne pathogen from the soy sauce braised fish-cake with quail-egg. | due to abundant contamination in various foods, the pathogenesis of bacillus cereus has been widely studied in physiological and molecular level. b. cereus forc_005 was isolated from a korean side dish, soy sauce braised fish-cake with quail-egg in south korea. while 21 complete genome sequences of b. cereus has been announced to date, this strain was completely sequenced, analyzed, and compared with other complete genome sequences of b. cereus to elucidate the distinct pathogenic features of a ... | 2015 | 26566422 |
| antibacterial action against food-borne microorganisms and antioxidant activity of carvacrol-rich oil from lippia origanoides kunth. | lippia origanoides kunth from northeast brazil is a plant of pleasant odor used by local people as a food seasoning in substitution the oregano where its carvacrol-rich oil has showed significant antimicrobial activity against human pathogens. | 2015 | 26552874 |
| bacillus cereus adhesion to simulated intestinal mucus is determined by its growth on mucin, rather than intestinal environmental parameters. | adhesion of pathogenic bacteria to intestinal mucus, the protective layer of the gastrointestinal epithelium, is often considered a virulence factor. the ability of food-poisoning bacillus cereus strains to attach to mucus and the factors affecting this interaction have not yet been investigated. therefore, the role of adhesion in pathogenesis of b. cereus still remains unknown. in the present study, an in vitro assay based on mucin agar was used to simulate adhesion of b. cereus to mucus. bacte ... | 2015 | 26545170 |
| [screening of molluscacidal microorganisms against oncomelania hupensis and their effect]. | fifteen soil samples were collected from oncomelania hupensis culture pond in miluo schistosomiasis control and prevention base, hunan province. four strains of bacteria were identified to have molluscacidal effects, numbered as b8, b27, b36 and b59. compared with the fermentation broth groups and bacteria suspension groups, the fermentation supernatant groups of the four strains showed the strongest molluscacidal effect. the fermentation supernatant of b59 strain showed the best molluscacidal e ... | 2015 | 26541047 |
| comparison of primary models to predict microbial growth by the plate count and absorbance methods. | the selection of a primary model to describe microbial growth in predictive food microbiology often appears to be subjective. the objective of this research was to check the performance of different mathematical models in predicting growth parameters, both by absorbance and plate count methods. for this purpose, growth curves of three different microorganisms (bacillus cereus, listeria monocytogenes, and escherichia coli) grown under the same conditions, but with different initial concentrations ... | 2015 | 26539483 |
| identification of novel tylosin analogues generated by a wbla disruption mutant of streptomyces ansochromogenes. | streptomyces, as the main source of antibiotics, has been intensively exploited for discovering new drug candidates to combat the evolving pathogens. disruption of wbla, an actinobacteria-specific gene controlling major developmental transition, can cause the alteration of phenotype and morphology in many species of streptomyces. one wbla homologue was found in streptomyces ansochromogenes 7100 by using the basic local alignment search tool. it is interesting to identify whether novel secondary ... | 2015 | 26525981 |
| heavy metal detoxification by different bacillus species isolated from solar salterns. | the biosorption mechanism is an alternative for chemical precipitation and ultrafiltration which have been employed to treat heavy metal contamination with a limited success. in the present study, three species of bacillus which were isolated from solar salterns were screened for their detoxification potential of the heavy metals, lead, chromium, and copper, by biosorption. biosorption potential of each isolate was determined by atomic absorption spectroscopy (aas), inductively coupled plasma-op ... | 2015 | 26525498 |
| application of a rapid knowledge synthesis and transfer approach to assess the microbial safety of low-moisture foods. | low-moisture foods (lmf) are increasingly implicated in outbreaks of foodborne illness, resulting in a significant public health burden. to inform the development of a new codex alimentarius code of hygienic practice for lmf, we applied a rapid knowledge synthesis and transfer approach to review global research on the burden of illness, prevalence, and interventions to control nine selected microbial hazards in eight categories of lmf. knowledge synthesis methods included an integrated scoping r ... | 2015 | 26613924 |
| changes in the microbial composition of microbrewed beer during the process in the actual manufacturing line. | this study investigated changes in the microbial composition of microbrewed beer during the manufacturing processes and identified potential microbial hazards, effective critical quality control points, and potential contamination routes. comprehensive quantitative (aerobic plate count, lactic acid bacteria, fungi, acetic acid bacteria, coliforms, and bacillus cereus) and qualitative (escherichia coli and eight foodborne pathogens) microbiological analyses were performed using samples of raw mat ... | 2015 | 26613919 |
| root associated bacillus sp. improves growth, yield and zinc translocation for basmati rice (oryza sativa) varieties. | plant associated rhizobacteria prevailing in different agro-ecosystems exhibit multiple traits which could be utilized in various aspect of sustainable agriculture. two hundred thirty four isolates were obtained from the roots of basmati-385 and basmati super rice varieties growing in clay loam and saline soil at different locations of punjab (pakistan). out of 234 isolates, 27 were able to solubilize zinc (zn) from different zn ores like zinc phosphate [zn3 (po4)2], zinc carbonate (znco3) and z ... | 2015 | 26635754 |
| silver nanoparticles cause complications in pregnant mice. | silver nanoparticles (agnps) have attracted much interest and have been used for antibacterial, antifungal, anticancer, and antiangiogenic applications because of their unique properties. the increased usage of agnps leads to a potential hazard to human health. however, the potential effects of agnps on animal models are not clear. this study was designed to investigate the potential impact of agnps on pregnant mice. | 2015 | 26622177 |
| phytofabrication of bioinduced silver nanoparticles for biomedical applications. | synthesis of nanomaterials holds infinite possibilities as nanotechnology is revolutionizing the field of medicine by its myriad applications. green synthesis of nanoparticles has become the need of the hour because of its eco-friendly, nontoxic, and economic nature. in this study, leaf extract of rosa damascena was used as a bioreductant to reduce silver nitrate, leading to synthesis of silver nanoparticles (agnps) in a single step, without the use of any additional reducing or capping agents. ... | 2015 | 26648715 |
| the endospore-forming pathogen bacillus cereus exploits a small colony variant-based diversification strategy in response to aminoglycoside exposure. | bacillus cereus is among the microorganisms most often isolated from cases of food spoilage and causes gastrointestinal diseases as well as nongastrointestinal infections elicited by the emetic toxin cereulide, enterotoxins, and a panel of tissue-destructive virulence factors. this opportunistic pathogen is increasingly associated with rapidly fatal clinical infections especially linked to neonates and immunocompromised individuals. fatality results from either the misdiagnosis of b. cereus as a ... | 2015 | 26646008 |
| structure elucidation, antimicrobial and cytotoxic activities of a halimane isolated from vellozia kolbekii alves (velloziaceae). | a new halimane diterpene was isolated from vellozia kolbekii alves (velloziaceae) and identified as (5r,8r,9s,13r)-halim-1,10-ene-15,16-diol (1). it showed cytotoxicity against three human cancer cell lines, sf-295 (glioblastoma), mda-mb-435 (melanoma), and hct-8 (colon adenocarcinoma). in the mechanism of cytotoxic action, halimane 1 interferes in two major phases of the cell cycle: in s phase, in which dna synthesis occurs and where it is very sensitive to damage, and g2m phase which is the ph ... | 2015 | 26663842 |
| genome sequence of a chromium-reducing strain, bacillus cereus s612. | we report here the genome sequence of an effective chromium-reducing bacterium, bacillus cereus strain s612. the size of the draft genome sequence is approximately 5.4 mb, with a g+c content of 35%, and it is predicted to contain 5,450 protein-coding genes. | 2015 | 26659672 |
| molecular characterization and biological effects of a c-type lectin-like receptor in large yellow croaker (larimichthys crocea). | the c-type lectin-like receptors (ctlrs) play important roles in innate immunity as one type of pattern recognition receptors. here, we cloned and characterized a c-type lectin-like receptor (lycctlr) from large yellow croaker larimichthys crocea. the full-length cdna of lycctlr is 880 nucleotides long, encoding a protein of 215 amino acids. the deduced lycctlr contains a c-terminal c-type lectin-like domain (ctld), an n-terminal cytoplasmic tail, and a transmembrane region. the ctld of lycctlr ... | 2015 | 26690423 |