Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| comparison of five antimicrobial susceptibility tests in detecting high level aminoglycoside and vancomycin resistances in hospital acquired enterococcus isolates. | multiple antibiotic resistance is increasing in the genus enterococcus. the aim of this research is to compare five different antibiotic susceptibility test methods used in enterococcal isolates. | 2011 | 21500722 |
| conservation of ebp-type pilus genes among enterococci and demonstration of their role in adherence of enterococcus faecalis to human platelets. | ebp are endocarditis and biofilm associated pili of enterococcus faecalis that are also important in experimental utis. our analyses, using available genomes, found that the ebp locus is unique to enterococci. in e. faecalis, the ebp locus is very highly conserved and only 1/473 e. faecalis isolates tested lacked ebpabc while only 1.2% had the bee pilus locus. no other pilus-encoding operon was identified in 55 available genomes indicating that the vast majority of e. faecalis (unlike enterococc ... | 2011 | 21502588 |
| genetic basis for daptomycin resistance in enterococci. | the emergence of multidrug resistant enterococci as leading causes of hospital acquired infection is an important public health concern. little is known about the genetic mechanisms by which enterococci adapt to strong selective pressures, including the use of antibiotics. the lipopeptide antibiotic daptomycin is approved to treat gram-positive bacterial infections including those caused by enterococci. since its introduction, resistance to daptomycin by strains of enterococcus faecalis and e. f ... | 2011 | 21502617 |
| genotypic analysis of enterococci isolated from fecal-polluted water from different sources by pulsed-field gel electrophoresis (pfge) for application to microbial source tracking. | pfge has potential applications in the source tracking of fecal pollution in aquatic environments. we tried to distinguish the genotypes of enterococcus faecium collected from fecal-contaminated water using pfge. well identified 115 strains of e. faecium were classified into 25 pfge patterns, and characteristics distinctive to each genotype were recognized. analysis of the characteristics of genotypes using pfge can be used to track source of fecal pollution. | 2011 | 21502739 |
| sitafloxacin: in bacterial infections. | sitafloxacin is a fluoroquinolone antibacterial with in vitro activity against a broad range of gram-positive and -negative bacteria, including anaerobic bacteria, as well as against atypical pathogens. it is approved in japan for use in a number of bacterial infections caused by sitafloxacin-susceptible strains of staphylococcus spp., streptococcus pneumoniae, other streptococcus spp., enterococcus spp., moraxella catarrhalis, escherichia coli, citrobacter spp., klebsiella spp., enterobacter sp ... | 2011 | 21504249 |
| relative contributions of ebp pili and the collagen adhesin ace to host extracellular matrix protein adherence and experimental urinary tract infection by enterococcus faecalis og1rf. | previous studies have demonstrated that the ebp operon and the ace gene of enterococcus faecalis, encoding endocarditis- and biofilm-associated pili and an adhesin to collagen of e. faecalis, respectively, are both important in experimental urinary tract infections (uti) and endocarditis. we have also shown that growth of e. faecalis in bhi-serum enhances ebp pilus and ace production and increases adherence to several host extracellular matrix proteins. here, we report that deletion of ebpabc al ... | 2011 | 21505082 |
| comparative analysis of the bacterial flora of vegetables collected directly from farms and from supermarkets in germany. | a total of 1,001 vegetables were collected from 13 farms and 11 supermarkets in bavaria, germany; 722 samples were positive for coliforms (mostly enterobacter cloacae; n = 176). escherichia coli were detected in 34, pseudomonas spp. in 439, salmonella spp. in 1, enterococcus spp. in 682, and listeria spp. in 11 samples. prevalence of all investigated genera tended to be lower in samples collected at the supermarket. however, prevalence of pseudomonas fluorescens was higher in supermarket samples ... | 2011 | 21506036 |
| a comparative evaluation of the sealing ability of 2 root-end filling materials: an in vitro leakage study using enterococcus faecalis. | objective: the purpose of this study was to evaluate the sealing ability of endosequence bioceramic root-end repair (bcrr) material when compared with white mineral trioxide aggregate (wmta). study design: forty single-rooted teeth were instrumented, obturated with gutta-percha, root-end resected, and retrofilled with 2 different materials: white proroot mta (wmta) (n = 15) and bcrr (n = 15). unfilled specimens (n = 10) received no retrofill and were used as controls. all groups received e. faec ... | 2011 | 21507688 |
| gilthead seabream (sparus aurata) carrying antibiotic resistant enterococci. a potential bioindicator of marine contamination? | antibiotic resistance in bacteria is a growing problem that is not only restricted to the clinical setting but also to other environments such as marine species that harbor antibiotic resistant bacteria and therefore may serve as reservoirs for antibiotic-resistance genetic determinants. the aim of this study was to evaluate antibiotic resistance phenotypes in enterococci isolated from fecal samples of gilthead seabream and the associated mechanisms of resistance. a collection of 118 samples wer ... | 2011 | 21511306 |
| changes in faecal bacteria and metabolic parameters in foals during the first six weeks of life. | many foals develop diarrhoea within the first two weeks of life which has been suggested to coincide with postpartum oestrus in their dams. to analyse the pathogenesis of this diarrhoea we have determined faecal bacteria in foals and their dams (n=30 each), and serum igf-1 and γ-globulins for 6 weeks after birth. in addition, effects of β-carotene supplementation to mares (group 1: 1000mg/day, n=15, group 2: control, n=15) on diarrhoea in foals were studied. diarrhoea occurred in 92 and 79% of f ... | 2011 | 21511405 |
| in vitro activity and in vivo efficacy of the saponin hsm1 alone and in combination with daptomycin and vancomycin against gram-positive cocci. | surgical site infections are the second most common cause of hospital- and community-acquired gram-positive infections, the us centers for diseases control and prevention (cdc) estimates that about 500,000 surgical site infections occur annually in the united states. the aim of the work was to determine the in vitro activity of the saponin named diosgenyl 2-amino-2-deoxy-beta-d-glucopyranoside hydrochloride (hsm1) and its bactericidal effect for a large number of gram-positive cocci, as well as ... | 2011 | 21511888 |
| isolated cholangiolitis revealed by (18)f-fdg-pet/ct in a patient with fever of unknown origin. | cholangiolitis, inflammation of the cholangioles, is difficult to diagnose by conventional imaging modalities. we report a case of cholangiolitis revealed by fluorine-18 fluoro desoxyglucose positron emission tomography-computerized tomography ((18)f-fdg-pet/ct) after about 9 months of recurrent fevers. a 20 years old girl with a history of recurrent fevers and repeated workups at different hospitals, which didn't diagnosed the source of fever, was admitted with a recent episode of fever. an (18 ... | 2011 | 21512668 |
| rare case of large double perforated anterior mitral leaflet pseudoaneurysm in a patient with aortic valve endocarditis. | 2011 | 21512979 | |
| systematic review of measurement and adjustment for colonization pressure in studies of methicillin-resistant staphylococcus aureus, vancomycin-resistant enterococci, and clostridium difficile acquisition. | colonization pressure is an important infection control metric. the aim of this study was to describe the definition and measurement of and adjustment for colonization pressure in nosocomial-acquisition risk factor studies of methicillin-resistant staphylococcus aureus (mrsa), vancomycin-resistant enterococci (vre), and clostridium difficile. | 2011 | 21515979 |
| molecular epidemiology and risk factors for colonization by vancomycin-resistant enterococcus in patients with hematologic malignancies. | to study the molecular epidemiology of vancomycin-resistant enterococcus (vre) colonization and to identify modifiable risk factors among patients with hematologic malignancies. | 2011 | 21515980 |
| chicken intestine microbiota following the administration of lupulone, a hop-based antimicrobial. | the use of antibiotic growth promotants in poultry rearing is a public health concern due to antibiotic resistance in bacteria and the harborage of resistance genes. lupulone, a hop β-acid from humulus lupulus, has been considered as a potential feed additive growth promotant. here, the effect of lupulone was evaluated for its effect on the microbiota of the chicken intestine. the intestinal microbiota of broilers was quantified after the addition of 125 mg l(-1) lupulone to water and challenge ... | 2011 | 21517917 |
| inhibitory effects of enterococci on the production of hydrogen sulfide by hydrogen sulfide-producing bacteria in raw meat. | aims: applying competitive exclusion micro-organisms to control hydrogen sulfide (h(2) s) gas produced by hydrogen sulfide-producing bacteria (spb) in chicken meat. methods and results: five spb strains, isolated from animal by-products, were used for screening lactic acid bacteria (lab) that can inhibit the production of h(2) s by spb in trypticase soy broth supplemented with l-cysteine (tsb-l-cys). a sensitive and accurate test strip method was developed for h(2) s determination in real time ... | 2011 | 21518155 |
| bacterial monocultures, propionate, butyrate and h(2) o(2) modulate the expression, secretion and structure of the fasting-induced adipose factor in gut epithelial cell lines. | previous research showed that an intestinal microbial community represses the fasting-induced adipose factor (fiaf) in the gut epithelium, thereby increasing fat storage in the host. this study was designed to investigate the overall effect of different bacterial species and metabolites on fiaf in intestinal (caco-2, ht-29 and hct-116) and hepatic (hepg2) cancer cell lines. first, we showed that fiaf was present in different isoforms, and secreted as n-glycosylated proteins, exclusively at the b ... | 2011 | 21518214 |
| identification of malic and soluble oxaloacetate decarboxylase enzymes in enterococcus faecalis. | two paralogous genes, maee and citm, that encode putative malic enzyme family members were identified in the enterococcus faecalis genome. maee (41 kda) and citm (42 kda) share a high degree of homology between them (47% identities and 68% conservative substitutions). however, the genetic context of each gene suggested that maee is associated with malate utilization whereas citm is linked to the citrate fermentation pathway. in the present work, we focus on the biochemical characterization and p ... | 2011 | 21518252 |
| effects of genetically modified t2a-1 rice on faecal microflora of rats during 90 day supplementation. | background: many animal studies have been performed on products with the bacillus thuringiensis insecticidal toxin-encoding gene (bt products), but less have focused on its effects on intestinal microflora owing to difficulties in culturing. this 90 day study was designed to assess unintended effects of genetically modified t2a-1 rice (gmr) on selected intestinal bacteria (lactobacillus group, bifidobacterium genus, escherichia coli subgroup, enterococcus genus and clostridium perfringens) of ra ... | 2011 | 21520451 |
| rapid identification of bacteria with a disposable colorimetric sensing array. | rapid identification of both species and even specific strains of human pathogenic bacteria grown on standard agar has been achieved from the volatiles they produce using a disposable colorimetric sensor array in a petri dish imaged with an inexpensive scanner. all 10 strains of bacteria tested, including enterococcus faecalis and staphylococcus aureus and their antibiotic-resistant forms, were identified with 98.8% accuracy within 10 h, a clinically important time frame. furthermore, the colori ... | 2011 | 21524080 |
| antimicrobial effectiveness of different preparations of calcium hydroxide. | background: intracanal medicaments have an antibacterial effect on the root canal flora. the effectiveness of such vehicles has to be tested and substantiated against the normal bacterial flora. the various vehicles that were tested for their effectiveness were commercially prepared calcium hydroxide (metapex), saline, glycerine, cmcp, and rexidine-m gel. the bacterial cultures tested for sensitivity were aerobes (staphylococcus aureus, streptococcus viridans, streptococcus pyogens, enterococcus ... | 2011 | 21525680 |
| a family of multicyclic indolosesquiterpenes from a bacterial endophyte. | three novel indolosesquiterpenes, xiamycin b (1b), indosespene (2), and sespenine (3), along with the known xiamycin a (1a) were isolated from the culture broth of streptomyces sp. hki0595, a bacterial endophyte of the widespread mangrove tree kandelia candel. agar diffusion assays revealed moderate to strong antimicrobial activities against several bacteria, including methicillin-resistant staphylococcus aureus and vancomycin-resistant enterococcus faecalis, while no cytotoxicity against human ... | 2011 | 21528153 |
| epidemiology of clostridium difficile and vancomycin-resistant enterococcus colonization in patients on a spinal cord injury unit. | patients with spinal cord injury (sci) are at risk of acquiring colonization with clostridium difficile and vancomycin-resistant enterococcus (vre) due to prolonged hospitalization and frequent antimicrobial use. we examined the frequency of stool, skin, and environmental contamination with c. difficile and vre in hospitalized patients with scl. | 2011 | 21528623 |
| risk factors for elevated enterococcus concentrations in a rural tropical island watershed. | associations were examined between riparian canopy cover, presence of cattle near streams, and month of year with the concentration of enterococcus (most probable number (mpn)/100 ml) in surface water at waipā watershed on the north side of the hawaiian island kaua'i. each one percent decrease in riparian canopy cover was associated with a 3.6 mpn/100 ml increase of waterborne enterococcus. presence of cattle near monitoring sites was associated with an increase of 99.3 mpn/100 ml of enterococcu ... | 2011 | 21530065 |
| the influence of cone-beam computed tomography and periapical radiographic evaluation on the assessment of periapical bone destruction in dog's teeth. | objective: the aim of this study was to determine the influence of periapical radiographs, cone beam computed tomography (cbct) sections, and cone beam volumetric data on the determination of periapical bone destruction in endodontically treated distal root canals of premolar canine teeth. nontreated mesial roots were used as controls. study design: enterococcus faecalis strain (atcc 29212) was inoculated into 30 root canals of 2 mongrel dogs to induce apical periodontitis. after 60 days, the ro ... | 2011 | 21530334 |
| antibacterial efficacy of endodontic irrigating solutions and their combinations in root canals contaminated with enterococcus faecalis. | objective: the objective of this study was to evaluate the antibacterial efficacy of irrigating solutions and their combinations against enterococcus faecalis. study design: one hundred ten single-rooted human teeth were inoculated with e. faecalis and incubated for 21 days. teeth were divided according to the irrigant: group i (gi), 2.5% sodium hypochlorite solution (naocl); gii, 2.5% naocl + 10% citric acid; giii, 2.5% naocl + apple cider vinegar; giv, apple cider vinegar; gv, 2% chlorhexidine ... | 2011 | 21531598 |
| clinical course and outcomes of single-organism enterococcus peritonitis in peritoneal dialysis patients. | background and objectives: enterococci are part of the normal flora of the gastrointestinal tract. they can cause enteric peritonitis, which is a serious complication of peritoneal dialysis (pd). however, the clinical course and outcome of pd-related enterococcus peritonitis remainsunclear. ♢ methods: we reviewed all enterococcus peritonitis episodes occurring in our dialysis unit from 1995 to 2009. ♢ results: during the study period, 1421 episodes of peritonitis were recorded. of 29 episodes (2 ... | 2011 | 21532006 |
| properties and applications of calcium hydroxide in endodontics and dental traumatology. | mohammadi z, dummer pmh. properties and applications of calcium hydroxide in endodontics and dental traumatology. international endodontic journal. abstract: calcium hydroxide has been included within several materials and antimicrobial formulations that are used in a number of treatment modalities in endodontics. these include, inter-appointment intracanal medicaments, pulp-capping agents and root canal sealers. calcium hydroxide formulations are also used during treatment of root perforations, ... | 2011 | 21535021 |
| potential systematic error in laboratory experiments on microbial leakage through filled root canals: an experimental study. | rechenberg d-k, thurnheer t, zehnder m. potential systematic error in laboratory experiments on microbial leakage through filled root canals: an experimental study. international endodontic journal. abstract: aim to assess the routes of bacterial leakage in a commonly used two-chamber model designed to evaluate root fillings. methodology fifty-one intact human mandibular premolars with fully developed roots were used. they were left completely intact (n = 23), or were accessed, instrumented an ... | 2011 | 21535022 |
| slya is a transcriptional regulator involved in the virulence of enterococcus faecalis. | phylogenetic analysis of the crystal structure of the enterococcus faecalis slya (ef_3002) transcriptional factor places it between the slya and marr regulator subfamilies. proteins of these families are often involved in the regulation of genes important for bacterial virulence and stress response. to gather evidence for the role of this putative regulator in e. faecalis biology, we dissected the genetic organisation of the slya-ef_3001 locus, and constructed slya deletion mutant as well as com ... | 2011 | 21536798 |
| in vitro antimicrobial efficacy of a silver alginate dressing on burn wound isolates. | to test the antimicrobial effectiveness of a silver alginate dressing on opportunistic pathogens, namely meticillin-sensitive staphylococcus aureus (mssa) and meticillin-resistant staphylococcus aureus (mrsa), klebsiella spp., enterococcus faecalis, enterococcus faecium, pseudomonas aeruginosa, escherichia coli, enterobacter sakazakii, enterobacter cloacae, serratia marcescens, chryseobacterium indologenes, proteus vulgaris and acinetobacter baumannii. | 2011 | 21537295 |
| acquisition of vanb-type vancomycin resistance by bacillus subtilis: the impact on gene expression, cell wall composition and morphology. | the vancomycin resistance operons from enterococci, staphylococci and actinomycetes encode a vanrs two-component signal transduction system (tcs) and a suite of enzymes to modify the peptidoglycan biosynthetic precursor lipid ii and to eliminate the d-ala-d-ala from the cell. commingling of these regulatory and enzymatic activities with host functions has the potential to significantly impact host gene expression and cell wall metabolism. here we report the effects of individually expressing the ... | 2011 | 21542863 |
| inactivation kinetics of a new target of {beta}-lactam antibiotics. | peptidoglycan was previously thought to be predominantly cross-linked by serine d,d-transpeptidases that are the target of β-lactam antibiotics. however, unrelated cysteine l,d-transpeptidases have been recently recognized as a predominant mode of peptidoglycan cross-linking in mycobacterium tuberculosis and as a by-pass mechanism conferring resistance to all β-lactams, except carbapenems such as imipenem, in enterococcus faecium. investigation of the mechanism of inhibition of this new β-lactam ... | 2011 | 21543331 |
| reevaluation of the taxonomic status of recently described species of enterococcus: evidence that enterococcus thailandicus is a senior subjective synonym of "enterococcus sanguinicola", and confirmation of enterococcus caccae as a species distinct from enterococcus silesiacus. | several of the more recently proposed new species of enterococcus are nearly identical based on 16s rdna sequence analysis and phenotypic traits. in the present study, dna-dna reassociation experiments, in conjunction with sequencing of the 16s rrna and rpob genes, provided evidence that "enterococcus sanguinicola" and enterococcus thailandicus actually represent the same species. in contrast, enterococcus caccae and enterococcus silesiacus, two other species with nearly identical 16s rrna gene ... | 2011 | 21543565 |
| pathogenic gram-positive cocci in south african rainbow trout, oncorhynchus mykiss (walbaum). | 2011 | 21545442 | |
| biofilm formation and adherence characteristics of an elizabethkingia meningoseptica isolate from oreochromis mossambicus. | abstract: background: elizabethkingia spp. are opportunistic pathogens often found associated with intravascular device-related bacteraemias and ventilator-associated pneumonia. their ability to exist as biofilm structures has been alluded to but not extensively investigated. methods: the ability of elizabethkingia meningoseptica isolate ch2b from freshwater tilapia (oreochromis mossambicus) and e. meningoseptica strain nctc 10016t to adhere to abiotic surfaces was investigated using microtiter ... | 2011 | 21545730 |
| effectiveness of a novel ozone-based system for the rapid high-level disinfection of health care spaces and surfaces. | background: vapor-based fumigant systems for disinfection of health care surfaces and spaces is an evolving technology. a new system (asepticsure) uses an ozone-based process to create a highly reactive oxidative vapor with broad and high-level antimicrobial properties. methods: ozone gas at 50-500 ppm was combined with 3% hydrogen peroxide vapor in a test chamber and upscaled in rooms measuring 82 m(3) and 90 m(3) in area. test organisms included methicillin-resistant staphylococcus aureus, van ... | 2011 | 21546123 |
| antimicrobial susceptibility of daptomycin and comparator agents tested against methicillin-resistant staphylococcus aureus and vancomycin-resistant enterococci: trend analysis of a 6-year period in us medical centers (2005-2010). | we evaluated daptomycin activity trends against methicillin-resistant staphylococcus aureus (mrsa) and vancomycin-resistant enterococci (vre; mic, ≥8 μg/ml) in a 6-year period (2005-2010) following us regulatory release for clinical use. consecutive, unique patient strains of clinical significance were collected in 32 us medical centers and susceptibility tested in a central laboratory against daptomycin and various comparator agents by reference broth microdilution methods. a total of 22 858 s. ... | 2011 | 21546202 |
| fluoroquinolone-resistant acute prostatitis requiring hospitalization after transrectal prostate biopsy: effect of previous fluoroquinolone use as prophylaxis or long-term treatment. | objectives: this study aims to scrutinize the hospitalized patients with the diagnosis of acute prostatitis after transrectal ultrasound-guided biopsy of the prostate (trusbp) focusing their history of previous antibiotic use, clinical pictures, microbiologic features, and resistance patterns of the isolates. patients and methods: a retrospective evaluation of the records between 2005 and 2010 revealed 13 patients. all patients received ciprofloxacin 500 mg twice a day starting from the day befo ... | 2011 | 21547465 |
| azaphilones from the endophyte chaetomium globosum. | six new azaphilones, 5'-epichaetoviridin a (7), 4'-epichaetoviridin f (9), 12β-hydroxychaetoviridin c (10), and chaetoviridins g-i (11-13), and six known azaphilones, chaetoviridins a-e (1-5) and 4'-epichaetoviridin a (8), were isolated from the endophytic fungus chaetomium globosum cultivated in pdb medium for 21 days. the structure elucidation and the assignment of the relative configurations of the new natural products were based on detailed nmr and ms spectroscopic analyses. the structure of ... | 2011 | 21548578 |
| pathogenic fungi: an unacknowledged risk at coastal resorts? new insights on microbiological sand quality in portugal. | whilst the potential impact on beach users from microorganisms in water has received considerable attention, there has been relatively little investigation into microbial contaminants in sand. thirty three beaches across portugal were analyzed during a five year period (2006-2010) to determine the presence of yeasts, pathogenic fungi, dermatophytes, total coliforms, escherichia coli and intestinal enterococci in sand. our results showed that 60.4% of the samples were positive for fungi and that ... | 2011 | 21550072 |
| in vitro activity of new antimicrobial agents against glycopeptide-resistant enterococcus faecium clinical isolates from france between 2006 and 2008. | objective: the aim of this study was to determine the in vitro activity of six new antimicrobial agents against glycopeptide-resistant enterococci (gre) strains from france. methods: sixty epidemiologically unrelated clinical isolates of enterococcus faecium (vana or vanb), received at the national reference centre for enterococci (cnr-enc) between 2006 and 2008, were studied. the mics of the following antibiotics were determined by broth microdilution according to antibiogram committee of the f ... | 2011 | 21550192 |
| n-benzyl-3-sulfonamidopyrrolidines are a new class of bacterial dna gyrase inhibitors. | this paper characterizes n-benzyl-3-sulfonamidopyrrolidines (gyramides) as dna gyrase inhibitors. gyramide a was previously shown to exhibit antimicrobial activity that suggested it inhibited bacterial cell division. in this study, we conducted target identification studies and identified dna gyrase as the primary target of gyramide a. the gyramide a resistance-determining region in dna gyrase is adjacent to the dna cleavage gate and is a new site for inhibitor design. we studied the antibiotic ... | 2011 | 21552338 |
| a case of bacteremia caused by hafnia paralvei. | the genus hafnia, a member of the enterobacteriaceae, is widespread in nature and rarely causes human infection. we describe a case of an 85-year-old japanese man hospitalized consequent to suspected cholecystitis, in which hafnia sp. was recovered from the blood culture concomitantly with enterococcus faecalis. sequencing of the 16s ribosomal rna gene sequence and phenotyping with id 32 e revealed that the recovered hafnia sp. was considered to be hafnia alvei genomosp. 2 (atcc 29927), recently ... | 2011 | 21553084 |
| empyema thoracis: analysis of 150 cases from a tertiary care centre in north east india. | objectives: to analyze the clinical characteristics, microbiological profile, management, complications and outcome of cases with empyema thoracis. methods: all cases diagnosed as 'empyema thoracis' over a period from january 2006 through june 2010 were identified from the inpatient records and discharge summaries. of the 160 cases identified, 150 cases were taken up for analysis and the rest 10 cases, of which two had significant predisposing co-morbidity and eight cases diagnosed as tubercular ... | 2011 | 21553207 |
| angioinvasive fusarium and concomitant enterococcus infection arising in association with leukemia cutis. | leukemia cutis represents the cutaneous infiltration of neoplastic leukocytes or their precursors that results in clinically identifiable skin lesions. for patients with myelodysplastic syndrome, developing such a lesion may indicate impending leukemic transition. these patients are also often immunocompromised, putting them at risk for infection by opportunistic fungal pathogens such as fusarium. we describe an 85-year-old man with myelodysplastic syndrome who presented with a reddish-purple no ... | 2011 | 21623868 |
| in vitro assay of the antimicrobial activity of kephir against bacterial and fungal strains. | kephir is a fermented carbonated refreshing milk, with a slightly acidic aromatic taste and creamy foam composition which contains lactobacilli, leuconostocci, acetic acid bacteria, lactostreptococci and yeasts. recent studies have demonstrated its antibacterial, immunostimulating, antitumoral and cholesterol-lowering activities. purpose: the purpose of this study was to investigate the antimicrobial activity of kephir against bacillus subtilis spp. spizizenii atcc 6633, staphylococcus aureus at ... | 2011 | 21624484 |
| do mobile phones of patients, companions and visitors carry multidrug-resistant hospital pathogens? | a cross-sectional study was conducted to determine bacterial colonization on the mobile phones (mps) used by patients, patients' companions, visitors, and health care workers (hcws). significantly higher rates of pathogens (39.6% vs 20.6%, respectively; p = .02) were found in mps of patients' (n = 48) versus the hcws' (n = 12). there were also more multidrug pathogens in the patents' mps including methicillin-resistant staphylococcus aureus, extended-spectrum β-lactamase-producing escherichia co ... | 2011 | 21624635 |
| [associated antimicrobial resistance in enterococcus spp. clinical isolates.] | introduction: the increasing prevalence of enterococci strains in hospitals, particularly among isolates of vancomycin-resistant enterococci (vre), poses important problems because of the limited effect of antimicrobial therapy for enterococcal infections. methods: this work presents a retrospective investigation of quantitative in vitro susceptibility data for the range of antimicrobials against enterococcus spp. isolates and evaluation of the association of resistance between antimicrobial age ... | 2011 | 21625802 |
| detection and characterization of a st6 clone of vanb2-enterococcus faecalis from three different hospitals in spain. | thirteen vancomycin-resistant and teicoplanin-susceptible enterococcus faecalis isolates were recovered from unrelated patients in three spanish hospitals from november 2009 to december 2010. all isolates carried the vanb2 gene, showed indistinguishable or closely-related pfge patterns and were ascribed to the sequence type st6 (included into the high-risk clonal-complex cc2). they showed a multiresistance phenotype (erythromycin, tetracycline, ciprofloxacin and high-level-resistance to streptom ... | 2011 | 21626450 |
| comparison of bacterial adhesion and cellular proliferation on newly developed three-dimensional scaffolds manufactured by rapid prototyping technology. | scaffolds used in the field of tissue engineering should facilitate the adherence, spreading, and ingrowth of cells as well as prevent microbial adherence. for the first time, this study simultaneously deals with microbial and tissue cell adhesion to rapid prototyping-produced 3d-scaffolds. the cell growth of human osteosarcoma cells (cal-72) over a time period of 3-11 days were examined on three scaffolds (plga, plla, plla-tcp) and compared to the adhesion of salivary microorganisms and represe ... | 2011 | 21626662 |
| comparative study among clinical and commensal isolates of enterococcus faecalis for presence of esp gene and biofilm production. | because of increasing difficulty in treating enterococcal infections, effort is being devoted to understanding factors that are responsible for causing nosocomial infection, with a focus toward targeting these factors with new therapeutics. evidence has emerged that the esp gene mediates biofilm formation in vitro, which helps the organism colonize and cause infection. | 2011 | 21628813 |
| the pioneer gut microbiota in human neonates vaginally born at term - a pilot study. | the pioneer microbiota of the neonate may affect future actions of the immune system. this study aimed to map the pioneer microbiota in healthy neonates vaginally born at term. a subgroup of neonates born large for gestational age (lga) was compared with the neonates appropriate for gestational age (aga). fecal samples were collected, within 48 hours after birth, from 79 neonates. quantitative polymerase chain reaction was used for enumeration of lactobacillus, a subgroup of lactobacillus common ... | 2011 | 21629156 |
| synthetic 3-arylideneflavanones as inhibitors of the initial stages of biofilm formation by staphylococcus aureus and enterococcus faecalis. | the antimicrobial activity of twenty two synthetic flavonoids is reported. among them three 3-arylideneflavanones, 2b, 2c, and 2i, were shown to be highly active against staphylococcus aureus, s. epidermidis, and enterococcus faecalis reference strains, with mic (minimal inhibitory concentration) values ranging from 4.68 microg/ml (14.3 microm) to 37.5 microg/ml (119.7 microm). the synergy of oxacillin and vancomycin with 2c, evaluated as fractional inhibitory concentration index (fici) was show ... | 2011 | 21630583 |
| survival of faecal indicator bacteria in treated pig manure stored in clay-covered heaps in vietnam. | small-scale pig farmers in vietnam typically store manure in heaps covered by a layer of clay. however, the hygienic quality of manure applied to agricultural soil is uncertain as storage time is determined by the need for soil fertilizer. this study aimed to assess how the storage of pig manure added straw, lime stone or urea in heaps covered by clay affected the survival of faecal bacteria. e. coli was reduced from 4log(10)cfu/g to below the detection limit within 2 weeks with 2% (w/w) urea (c ... | 2011 | 21636227 |
| oxalate-degrading capacities of lactic acid bacteria in canine feces. | in this study, lactic acid bacteria in canine feces were isolated and identified, and their oxalate-degrading capacities were evaluated. the oxalate-degrading capacities were determined for 24 of 47 (51.06%) lactic acid bacteria isolates. of these, 8 isolates [leuconostoc mesenteroides (rl75), lactococcus garvieae (cd2), lactococcus subsp. lactis (cs21), enterococcus faecium (cl71 and cl72), and enterococcus faecalis (cd14, cs62, and cd12)] degraded more than 5% of the oxalate present, while the ... | 2011 | 21641129 |
| prospective study on central venous line associated bloodstream infections. | objective to prospectively assess the incidence rates and characteristics of central venous line associated bloodstream infections (clabsi) in one institution. methods all patients with indwelling central venous catheters (cvc) between 1 april 2008 and 31 march 2009 were enrolled. the medical records of patients were reviewed and information on relevant characteristics entered into a standardised questionnaire. central laboratory records were regularly checked for positive blood cultures in stud ... | 2011 | 21642273 |
| decay of bacterial pathogens, fecal indicators, and real-time quantitative pcr genetic markers in manure amended soils. | this study examined persistence and decay of bacterial pathogens, fecal indicator bacteria (fib), and emerging real-time quantitative pcr (qpcr) genetic markers for rapid detection of fecal pollution in manure-amended agricultural soils. known concentrations of transformed green fluorescent protein-expressing escherichia coli o157:h7/pzs and red fluorescent protein-expressing salmonella enterica serovar typhimurium/pds were added to laboratory-scale manure-amended soil microcosms with moisture c ... | 2011 | 21642395 |
| removal of salmonella and indicator micro-organisms in integrated constructed wetlands treating agricultural wastewater. | the purpose of this study was to investigate the removal of pathogenic and indicator micro-organisms in integrated constructed wetland (icw) systems treating agricultural wastewater. nine icw's treating piggery (3) or dairy (6) wastewaters were sampled and indicator micro-organisms were enumerated in the influent as well as the effluent from the first, mid- and final cells. the presence/absence of salmonella was also determined and any salmonella isolates recovered were characterized. mean count ... | 2011 | 21644154 |
| [decrease of urinary tract infections following catheterization after the education of health care workers, introduction of protocols and surveillance lists]. | urinary tract infections (uti) following catheterization are the most common hospital-acquired infections, with their frequency amounting to 30-40% of all hospital infections. major percentage of this kind of infectious episodes can be prevented via active and continual education of health care workers (hcw), implementation of transparent protocols concerning installation and attendance of urinary catheters and regular control of catheterized patients through surveillance lists. this research sh ... | 2011 | 21644274 |
| prevalence of vanc vancomycin-resistant enterococci in the teaching hospitals of the university of debrecen, hungary. | vancomycin-resistant enterococci (vre) are common nosocomial pathogens; however, until now they have been rarely encountered in hungary. in the present study, we investigated the prevalence of vre in the teaching hospitals of the university of debrecen. of 7,271 enterococcus-containing clinical samples collected between 2004 and 2009, we identified 16 vre. species-specific polymerase chain reaction was used to detect enterococcus faecalis, enterococcus faecium, enterococcus casseliflavus, and en ... | 2011 | 21649462 |
| risk factors for prolonged carriage of vancomycin-resistant enterococcus faecium among patients in intensive care units: a case-control study. | the aim of this study was to identify the risk factors for prolonged carriage of vancomycin-resistant enterococcus faecium (vref) in intensive care units (icus). | 2011 | 21652622 |
| temporal development of the infant gut microbiota in immunoglobulin e-sensitized and nonsensitized children determined by the ga-map infant array. | at birth, the human infant gut is sterile, but it becomes fully colonized within a few days. this initial colonization process has a major impact on immune development. our knowledge about the correlations between aberrant colonization patterns and immunological diseases, however, is limited. the aim of the present work was to develop the ga-map (genetic analysis microbiota array platform) infant array and to use this array to compare the temporal development of the gut microbiota in ige-sensiti ... | 2011 | 21653746 |
| determination of disk diffusion and mic quality control guidelines for jnj-q2, a novel quinolone. | jnj-q2 is a novel fluorinated 4-quinolone in development for treatment of acute bacterial skin and skin structure infection and community-acquired bacterial pneumonia. this quality control (qc) study was performed to establish ranges for control strains: staphylococcus aureus atcc 29213 (0.004 to 0.015 μg/ml), enterococcus faecalis atcc 29212 (0.015 to 0.06 μg/ml), pseudomonas aeruginosa atcc 27853 (0.5 to 2 μg/ml and 17 to 23 mm), escherichia coli atcc 25922 (0.008 to 0.03 μg/ml and 30 to 36 mm ... | 2011 | 21653768 |
| vitek 2 and phoenix fail to detect high-level gentamicin-resistant enterococcus faecium isolates with aac-aph gene. | 2011 | 21654126 | |
| detection of tetracycline and macrolide resistance determinants in enterococci of animal and environmental origin using multiplex pcr. | an occurrence of resistance to tetracycline (tet) and erythromycin (ery) was ascertained in 82 isolates of enterococcus spp. of animal and environmental origin. using e test, 33 isolates were resistant to tet and three isolates to ery. using polymerase chain reaction (pcr; single and multiplex), the tet determinants tet(m) and tet(l) were detected in 35 and 13 isolates, respectively. twelve isolates carried both tet(m) and tet(l) genes. eight isolates possessed ermb gene associated with ery resi ... | 2011 | 21656006 |
| detection and characterization of pediocin pa-1/ach like bacteriocin producing lactic acid bacteria. | fifty-five bacteriocinogenic lactic acid bacteria (lab) isolated from seven different sources. eight isolates were found to produce pediocin pa-1 like bacteriocin as detected by pedb gene pcr and dot-blot hybridization. the culture filtrate (cf) activity of these isolates exhibited strong antilisterial, antibacterial activity against tested food-borne pathogens and lab. the identification and genetic diversity among the selected lab was performed by conventional morphological and molecular tools ... | 2011 | 21656248 |
| [infectious endocarditis]. | 2011 | 21656500 | |
| tn916-like genetic elements: a diverse group of modular mobile elements conferring antibiotic resistance. | antibiotic-resistant gram-positive bacteria are responsible for morbidity and mortality in healthcare environments. enterococcus faecium, enterococcus faecalis, staphylococcus aureus and streptococcus pneumoniae can all exhibit clinically relevant multidrug resistance phenotypes due to acquired resistance genes on mobile genetic elements. it is possible that clinically relevant multidrug-resistant clostridium difficile strains will appear in the future, as the organism is adept at acquiring mobi ... | 2011 | 21658082 |
| population biology of gram-positive pathogens: high-risk clones for dissemination of antibiotic resistance. | infections caused by multiresistant gram-positive bacteria represent a major health burden in the community as well as in hospitalized patients. staphylococcus aureus, enterococcus faecalis and enterococcus faecium are well-known pathogens of hospitalized patients, frequently linked with resistance against multiple antibiotics, compromising effective therapy. streptococcus pneumoniae and streptococcus pyogenes are important pathogens in the community and s. aureus has recently emerged as an impo ... | 2011 | 21658083 |
| monitoring horizontal antibiotic resistance gene transfer in a colonic fermentation model. | the human microbiota is suggested to be a reservoir of antibiotic resistance (abr) genes, which are exchangeable between transient colonizers and residing bacteria. in this study, the transfer of abr genes from enterococcus faecalis to listeria monocytogenes and to commensal bacteria of the human gut microbiota was demonstrated in a colonic fermentation model. in the first fermentation, an e. faecalis donor harboring the marked 50-kb conjugative plasmid pre25(*) and a chromosomal marker was co-i ... | 2011 | 21658089 |
| effect of antibiotic diversity on ventilator-associated pneumonia caused by eskape organisms. | the aim of this study was to test in the clinic whether antimicrobial diversity affects resistance of enterococcus faecium, staphylococcus aureus, klebsiella species, acinetobacter baumannii, pseudomonas aeruginosa, and enterobacter species (eskape) pathogens in ventilator-associated pneumonia (vap). | 2011 | 21659436 |
| enterococcus faecalis from patients with chronic periodontitis: virulence and antimicrobial resistance traits and determinants. | this study investigated the presence of virulence and resistance traits, as well as their genetic determinants in subgingival enterococcus faecalis from patients with chronic periodontitis. twenty-four e. faecalis strains from a previously multi-locus sequence typing (mlst)-characterized strain collection were examined for virulence-associated phenotypes, antimicrobial susceptibility, and virulence- and antimicrobial-resistant determinants. gelatinase, hemolysin, and biofilm production were dete ... | 2011 | 21660501 |
| non-susceptibility to tigecycline in enterococci from hospitalised patients, food products and community sources. | in this study, the in vitro activity of tigecycline against 1140 enterococci collected from humans, food products, animals and the environment in portugal (1996-2008) was analysed. ten isolates (seven enterococcus faecalis and three enterococcus spp.) non-susceptible to tigecycline (minimum inhibitory concentrations of 0.5-1.0 mg/l), which were also resistant to tetracycline and minocycline, were mostly observed in samples collected before the introduction of tigecycline in the therapeutic arsen ... | 2011 | 21664110 |
| neutral metallated and meso-substituted porphyrins as antimicrobial agents against gram-positive pathogens. | staphylococcus aureus is a bacterial pathogen that causes severe infections among humans. the increasing emergence of antibiotic resistance necessitates the development of new strategies to combat the spread of disease. one approach is photodynamic inactivation using porphyrin photosensitizers, which generate superoxide and other radicals in the presence of light, causing cell death via the oxidation of proteins and lipids. in this study, we analyzed a novel library of meso-substituted and metal ... | 2011 | 21667268 |
| enterococcus avium bacteremia: a 12-year clinical experience with 53 patients. | because enterococcus avium is rarely isolated from blood cultures, little is known about the clinical features and outcomes of bacteremia caused by this organism, formerly called "group q streptococcus". we retrospectively evaluated the clinical features and outcomes of patients with clinically significant bacteremia caused by e. avium presenting at a tertiary-care hospital in korea between february 1997 and february 2009. we identified 53 patients over the 12-year period; of these, 27 (50.9%) h ... | 2011 | 21667269 |
| leader program results for 2009: an activity and spectrum analysis of linezolid using 6,414 clinical isolates from 56 medical centers in the united states. | the leader program monitors the in vitro activity of linezolid in sampled u.s. medical centers using reference broth microdilution methods with supporting molecular investigations in a central laboratory design. this report summarizes data obtained in 2009, the 6th consecutive year of this longitudinal study. a total of 6,414 isolates from 56 medical centers in all nine census regions across the united states participated in 2009. for the six leading species/groups, the following linezolid mic(9 ... | 2011 | 21670176 |
| antimicrobial susceptibilities of commonly encountered bacterial isolates to fosfomycin determined by agar dilution and disk diffusion methods. | we studied the antimicrobial activity of fosfomycin against 960 strains of commonly encountered bacteria associated with urinary tract infection using standard agar dilution and disk diffusion methods. species studied included 3 common species of enterobacteriaceae, pseudomonas aeruginosa, acinetobacter baumannii, and stenotrophomonas maltophilia; methicillin-susceptible and -resistant staphylococcus aureus; and vancomycin-susceptible and resistant enterococcus faecalis and e. faecium. mics and ... | 2011 | 21670185 |
| effect of the antibiotic avilamycin on the structure of the microbial community in the jejunal intestinal tract of broiler chickens. | this study was conducted to determine whether avilamycin, which is used as a growth promoter in broiler chickens, would affect the structure of the bacterial community within the jejunal intestinal tract. sixty chickens were assigned to 2 groups: a control group and an avilamycin-treated group. the jejunal tissue from 4 chicks randomly sampled on d 1, 3, 7, 21, and 42 from each treatment group were investigated for changes in villus height, total mesophilic bacteria, lactic acid bacteria, and th ... | 2011 | 21673169 |
| anti-proliferative activity of meroditerpenoids isolated from the brown alga stypopodium flabelliforme against several cancer cell lines. | the sea constitutes one of the most promising sources of novel compounds with potential application in human therapeutics. in particular, algae have proved to be an interesting source of new bioactive compounds. in this work, six meroditerpenoids (epitaondiol, epitaondiol diacetate, epitaondiol monoacetate, stypotriol triacetate, 14-ketostypodiol diacetate and stypodiol) isolated from the brown alga stypopodium flabelliforme were tested for their cell proliferation inhibitory activity in five ce ... | 2011 | 21673894 |
| molecular characterization of antibiotic resistance in enterococci recovered from seagulls (larus cachinnans) representing an environmental health problem. | antimicrobial resistance and the mechanisms implicated were studied in 54 enterococci recovered from 57 seagull fecal samples. almost 78% of the recovered enterococci showed resistance against one or more antibiotics and these isolates were identified to the species level. e. faecium was the most prevalent species (52.4%). high percentages of erythromycin and tetracycline resistances were found among our isolates (95.2%), and lower percentages were identified to other antibiotics. most of the te ... | 2011 | 21674073 |
| disinfection of dentinal tubules with two different formulations of calcium hydroxide as compared to 2% chlorhexidine: as intracanal medicaments against enterococcus faecalis and candida albicans: an in vitro study. | to evaluate the disinfection of dentinal tubules using calcium hydroxide with propylene glycol and calcium hydroxide with iodoform in silicone oil, as compared to 2% chlorhexidine gel. | 2011 | 21814363 |
| the effect of 4% lignocaine gel, 5% amiloride hcl and 10% chlorpromazine on e.faecalis. | thorough disinfection of the root canal system is essential for the success of root canal therapy. enterococcus faecalis is the most frequently found species in persistent/secondary intracanal infection associated endodontic treatment failure. the aim of this study was to evaluate the disinfection of dentinal tubules using 10% chlorpromazine, 4% lignocaine gel, 5% amiloride hydrochloride in comparison with 2% chlorhexidine gel. | 2011 | 21814358 |
| an evaluation of the ability of the probiotic strain lactobacillus rhamnosus gg to eliminate the gastrointestinal carrier state of vancomycin-resistant enterococci in colonized children. | goals:: to evaluate the efficacy of lactobacillus rhamnosus gg (lgg) supplementation in eliminating the gastrointestinal carrier state of vancomycin-resistant enterococci (vre) in colonized children, and to evaluate the affect of the probiotic on lactobacillus spp. counts in the gastrointestinal tract. study:: a randomized, single-blind, placebo-controlled study. children (0 to 18 y old) hospitalized at the wards of the children's hospital who were diagnosed with gastrointestinal carrier state o ... | 2011 | 21814146 |
| structure of the rotor ring modified with n,n'-dicyclohexylcarbodiimide of the na+-transporting vacuolar atpase. | the prokaryotic v-atpase of enterococcus hirae, closely related to the eukaryotic enzymes, provides a unique opportunity to study the ion-translocation mechanism because it transports na(+), which can be detected by radioisotope ( ) experiments and x-ray crystallography. in this study, we demonstrated that the binding affinity of the rotor ring (k ring) for decreased approximately 30-fold by reaction with n,n(')-dicyclohexylcarbodiimide (dccd), and determined the crystal structures of na(+)-boun ... | 2011 | 21813759 |
| controlled performance evaluation of the diversilab repetitive-sequence-based genotyping system for typing multi-drug resistant healthcare-associated bacterial pathogens. | fast, reliable and versatile typing tools are essential to differentiate among related bacterial strains for epidemiological investigation and surveillance of healthcare-associated infection with multi-drug resistant (mdr) pathogens. the diversilab (dl) system is a semi-automated repetitive sequence-based pcr system designed for rapid genotyping. the dl system performance was assessed by comparing its reproducibility, typeability, discriminatory power, and concordance with pulsed field gel elect ... | 2011 | 21813717 |
| [urinary tract infection in patient after brain injury]. | intruduction: one of the most important problems in treating patients after head injury is urinary complication. the first place take urinary infections, which disturb rehabilitation process. clean intermittent catherization, bladder training and good care do not preserve infection. the purpose of our study was to present the frequency of urinary infections and main pathogens involved in these infections among patients treated in the department of rehabilitation after head injury. | 2011 | 21812229 |
| innate humoural immunity is related to eggshell bacterial load of european birds: a comparative analysis. | fitness benefits associated with the development of a costly immune system would include not only self-protection against pathogenic microorganisms but also protection of host offspring if it reduces the probability and the rate of vertical transmission of microorganisms. this possibility predicts a negative relationship between probabilities of vertical transmission of symbionts and level of immune response that we here explore inter-specifically. we estimated eggshell bacterial loads by cultur ... | 2011 | 21811780 |
| dogs leaving the icu carry a very large multi-drug resistant enterococcal population with capacity for biofilm formation and horizontal gene transfer. | the enterococcal community from feces of seven dogs treated with antibiotics for 2-9 days in the veterinary intensive care unit (icu) was characterized. both, culture-based approach and culture-independent 16s rdna amplicon 454 pyrosequencing, revealed an abnormally large enterococcal community: 1.4±0.8×10(8) cfu gram(-1) of feces and 48.9±11.5% of the total 16,228 sequences, respectively. the diversity of the overall microbial community was very low which likely reflects a high selective antibi ... | 2011 | 21811613 |
| a vegan or vegetarian diet substantially alters the human colonic faecal microbiota. | background/objectives:consisting of ~10(14) microbial cells, the intestinal microbiota represents the largest and the most complex microbial community inhabiting the human body. however, the influence of regular diets on the microbiota is widely unknown.subjects/methods:we examined faecal samples of vegetarians (n=144), vegans (n=105) and an equal number of control subjects consuming ordinary omnivorous diet who were matched for age and gender. we used classical bacteriological isolation, identi ... | 2011 | 21811294 |
| evaluation of the incidence of endophthalmitis after intravitreal injection of anti-vascular endothelial growth factor. | aims: to report the incidence of infectious and noninfectious endophthalmitis after intravitreal injection of anti-vascular endothelial growth factor (vegf) from a multicenter clinical trial in japan. methods: a retrospective multicenter review of the data of patients who received intravitreal anti-vegf injections between january 2007 and march 2011 was undertaken. cases with the clinical diagnosis of endophthalmitis resulting from intravitreal injection were identified and reviewed. results: a ... | 2011 | 21811052 |
| essential oil from aerial parts of of betonica grandiflora willd. from iran. | the chemical composition of the essential oil obtained from the aerial parts of betonica grandiflora willd., growing wild in iran, was analysed by gas chromatography-mass spectrometry for the first time. overall, 40 volatile components were identified on the basis of their mass spectra characteristics and retention indices in which ledol (21.8%), myrtenyl acetate (21.7%), eudesm-7(11)-en-4-ol (6.5%), trans-caryophyllene (5.5%), a-bisabolol (4.9%) and isolongifolol (4.5%) were the major constitue ... | 2011 | 21809955 |
| water quality of medium size watercourse under baseflow conditions: the case study of river sutla in croatia. | the study on medium size river sutla in croatia indicated considerable water contamination at specific sites during the baseflow period, probably associated to low flow-rate (0.73-68.8 m3 s(-1)), and consequently low dilution capacity of this river. various aspects of contamination were observed: increased conductivity to 1,000 micros cm(-1), decreased dissolved oxygen level to 50%, 4-5 degrees c increased water temperature, increased concentrations of several dissolved trace elements (e.g., max ... | 2011 | 21809782 |
| microbial contamination of groundwater at small community water supplies in finland. | the raw water quality and associations between the factors considered as threats to water safety were studied in 20 groundwater supplies in central finland in 2002-2004. faecal contaminations indicated by the appearance of escherichia coli or intestinal enterococci were present in five small community water supplies, all these managed by local water cooperatives. elevated concentrations of nutrients in raw water were linked with the presence of faecal bacteria. the presence of on-site technical ... | 2011 | 21809781 |
| [disintegration of lactobacilli and enterococci for obtaining cell wall fragments]. | the methods of gram-positive cells disintegration to obtain cell wall fragments have been described. it has been shown that the use of ultrasonic disintegrator uzd-500 at the frequency 22 khz during 30 min is optimal for disintegration of lactic acid bacteria cells. also the ability to use grinding of cells in liquid nitrogen (6 times to the complete evaporation) for obtaining the cell wall fragments has been demonstrated. | 2011 | 21809685 |
| a call to arms: the imperative for antimicrobial stewardship. | antimicrobial resistance is a major public health crisis. the prevalence of drug-resistant organisms, such as the emerging nap1 strain of clostridium difficile, now highly resistant to fluoroquinolones, acinetobacter species, klebsiella pneumoniae carbapenemase-producing organisms, and methicillin-resistant staphylococcus aureus, is increasing nationwide. the sources of antimicrobial resistance are manifold, but there is a well-documented causal relationship between antimicrobial use and misuse ... | 2011 | 21795727 |
| d-ala-d-ser vann-type transferable vancomycin resistance in enterococcus faecium. | enterococcus faecium ucn71, isolated from a blood culture, was resistant to low levels of vancomycin (mic, 16 µg/ml) but susceptible to teicoplanin (mic, 0.5 µg/ml). no amplification was observed with primers specific for the previously described glycopeptide resistance ligase genes but a pcr product, corresponding to a gene called vann, was obtained using degenerate primers and sequenced. the deduced vann protein was related (65% identity) to the d-alanine:d-serine vanl ligase. the organization ... | 2011 | 21807981 |
| gentamicin improves the activity of daptomycin and vancomycin against enterococcus faecalis in vitro and in an experimental foreign-body infection model. | for enterococcal implant-associated infections the optimal treatment regimen has not been defined. we investigated the activity of daptomycin, vancomycin and gentamicin (and their combinations) against enterococcus faecalis in vitro and in a foreign-body infection model. antimicrobial activity was investigated by time-kill and of growth-related heat production studies (microcalorimetry), as well as in a guinea pig model with subcutaneously implanted cages. infection was established by percutaneo ... | 2011 | 21807979 |
| an assessment of pathogen removal during composting of the separated solid fraction of pig manure. | the aim was to investigate pathogen survival during composting of pig manure solids with and without bulking agents in two trials of 56days duration, each with four treatments. salmonella was detected in the sawdust and straw bulking agents but was undetectable in the compost, except in one treatment at day 0. enteric indicator organisms were reduced by day 7 (p<0.001) and were undetectable in the final compost, except for coliform which were present at 3.66-4.43log(10)cfu/g. yeasts and moulds w ... | 2011 | 21807501 |