Publications

effect of the ltd4 receptor antagonist ly203647 on endotoxic shock sequelae in the rat.	this study investigated the effect of a novel ltd4 receptor antagonist ly203647 on salmonella enteritidis endotoxin-induced shock sequelae in anesthetized rats. ly203647 (30 mg/kg i.v.) or vehicle was given 10 min prior to endotoxin (10 mg/kg i.v.) or its vehicle, and the hematocrit, mean arterial pressure and circulating leukocyte counts were determined. ly203647 significantly inhibited endotoxin-induced hemoconcentration up to 90 min post-endotoxin (46.7 +/- 1.3 vs. 51.9 +/- 2.4% at 30 min pos ...	1991	1954979
serum and breast milk antibodies to food antigens in african mothers and relation to their diet.	results of a series of historical and recent studies of human milk and its protective antibodies against several bacterial and viral diseases and infantile diarrhea are presented. first, 1476 infants from lahore, pakistan from 3 socioeconomic groups, were observed and 300 mothers were given instruction on exclusive breast feeding. the prevalence of diarrhea indicated 60-80% protection to consistency of breast feeding, especially in summer when mothers replace breast milk with other liquids. h ...	1991	1808999
efficacy and safety of recombinant human erythropoietin to prevent the anaemias of prematurity. european randomized multicenter trial.		1991	2040194
serological activity of antigens isolated from 11 bacteroides vulgatus strains.	capsular (cps) and phenol water (pw) extracts were prepared from 11 serogroup specific strains of b. vulgatus isolated from normal gut flora. the extracts were active in immunodiffusion (id) and passive hemagglutination (ha) tests. there was no correlation between sugar and protein contents and serological activity of these extracts.	1991	1725095
disappearance of a putative da-neuron antibody following adrenal medulla transplantation: relationship to a striatal-derived da neuron trophic factor.		1990	2290972
pathology of mesenteric lymph nodes of buffaloes and cattle with special reference to salmonellosis.	enlarged mesenteric lymph nodes were collected from ninety buffaloes and sixty cattle slaughtered at faisalabad abattoir. among these, salmonellae were isolated from lymph nodes of 32 (21.33%) animals. maximum preponderance of salmonellosis was recorded in animals over two years of age. enlargement, pale to dark red in color, increased consistency and even calcification were the main gross pathological lesions. histopathological lesions included thickened capsule, typical lymphofollicular reacti ...	1990	2134150
non-immune hydrops in trisomy-18. diagnosis by vaginosonography and chorionic villus sampling in the first trimester. case report.		1990	2223690
non-immune hydrops in trisomy-18. diagnosis by vaginosonography and chorionic villus sampling in the first trimester. case report.		1990	2223690
application of brain microdialysis to study the pharmacology of the 5-ht1a autoreceptor.	5-hydroxytryptamine (5-ht) receptors of the 5-ht1a subtype are localized on serotoninergic cells and dendrites in the raphe nuclei of the brain stem and are believed to regulate synaptic 5-ht release through an inhibitory influence on serotoninergic impulse flow. the effects of 5-ht1a agonists on 5-ht release can, therefore, only be detected by measurement of 5-ht release from intact serotoninergic neurones. here we review the evidence that the microdialysis technique, when applied to the anaest ...	1990	2259248
[experimental study of liver allograft damage during cold ischemia].	changes on the majority of structural complexes were observed in livers that underwent the influence of the preservant and cold ischemia. cellular architecture of a such liver is disturbed. hepatocites are with granular and vacuolar degeneration: focal cariolyzes and piasmolyzes are present, partial destruction of cellular membrane is noticeable. the dilatation of sinusoidal spaces is very emphasized and followed by the severe loss of endothelium. these changes along with the disturbed cellular ...	1990	2327207
experimental studies on some effects of prostaglandin e2 in acute renal failure.	acute renal failure has been produced in rats with oral administration of uranyl nitrate 40 mg/kg of body weight once, and histopathological changes in the kidneys with blood biochemical values were studied by sacrificing them after 36 hours of feeding. prostaglandin e2 in 100 micrograms/kg of body weight was orally administered to another group of rats along with uranyl nitrate 40 mg/kg of body weight once and sacrificed them 36 hours after feeding. it was found that prostaglandin e2 has some p ...	1990	2362130
dietary modification of potential vitamin k supply from enteric bacterial menaquinones in rats.	rats given a low-fibre diet based on boiled white rice developed symptoms of severe vitamin k deficiency within 23 d. inclusion of autoclaved black-eye beans (vigna unguiculata) in the diet prevented the bleeding syndrome. to test the hypothesis that deficiency resulted from low phylloquinone intake exacerbated by inadequate production of menaquinones by the enteric bacteria, a follow-up experiment was carried out in which groups of rats were given an all-rice diet, a rice + beans diet or a stoc ...	1990	2383538
risk factors for kaposi's sarcoma in the vancouver lymphadenopathy-aids study.	in our ongoing cohort study of homosexual men, the ratio of new kaposi's sarcoma (ks) cases to new opportunistic infections (oi) during the periods 1982-1985, 1986-1987, and 1988-1989 fell from 0.75 (9 ks: 12 oi) to 0.57 (12 ks:21 oi) to 0.27 (4 ks:15 oi), respectively. to examine factors associated with the development of ks as compared to oi, we compared antecedent risk factors in 25 ks cases and 48 oi "controls." in univariate analyses, several classical hiv risk factors including numbers of ...	1990	2395081
serological studies of bacteroides vulgatus from normal gut flora.	a serological classification scheme for 48 strains of bacteroides vulgatus was established by agglutination technique. bacterial strains were isolated in our laboratory from gut flora of healthy persons. absorbed antisera to 11 strains of b. vulgatus were prepared. all 48 strains tested reacted with one or more antisera. among these strains, 22 serogroups were formed; 11 of them contained only one group component and 11 were made up of multiple group components.	1989	2482662
purification and characterization of a new hydrolase for conjugated bile acids, chenodeoxycholyltaurine hydrolase, from bacteroides vulgatus.	a new hydrolase for conjugated bile acids, tentatively named chenodeoxycholyltaurine hydrolase, was purified to homogeneity from bacteroides vulgatus. this enzyme hydrolyzed taurine-conjugated bile acids but showed no activity toward glycine conjugates. among the taurine conjugates, taurochenodeoxycholic acid was most effectively hydrolyzed, tauro-beta-muricholic and ursodeoxycholic acids were moderately well hydrolyzed, and cholic and 7 beta-cholic acids were hardly hydrolyzed, suggesting that ...	1989	2628421
dna alkylation and formation of dna interstrand cross-links by potential antitumour 2,5-bis(1-aziridinyl)-1,4-benzoquinones.	a series of 3,6-substituted 2,5-bis(1-aziridinyl)-1,4-benzoquinone derivatives was shown to alkylate calf thymus dna and to form dna interstrand cross-links. alkylation and cross-link formation were enhanced after electrochemical reduction of the compounds and increased with lower ph in the ph range from 4.5 to 8.0. reduction especially shifts the ph at which cross-linking and alkylation occurs to higher values, which are more physiologically relevant. this shift is probably caused by the increa ...	1989	2663197
in vitro susceptibility and in vivo efficacy of antimicrobials in the treatment of bacteroides fragilis-escherichia coli infection in mice.	cefamandole, cefoxitin, cefotetan, ceftizoxime, imipenem plus cilastatin, and ampicillin plus sulbactam were compared in the eradication of subcutaneous abscess in mice caused by bacteroides fragilis group organisms and escherichia coli alone or in combination. the abscesses were examined 5 d after inoculation. b. fragilis group reached log10.1-11.0 organisms per abscess and e. coli log11.6-12.5. imipenem plus cilastatin significantly reduced (in 6.9-10.6 logs) the number of e. coli and all memb ...	1989	2677161
pathogenicity of the bacteroides fragilis group.	the bacteroides fragilis group is one of the most important pathogens in polymicrobial infections. the distribution of the different members of the b. fragilis group in clinical infections varies. bacteroides fragilis accounts for 63 percent of all the group isolates, bacteroides thetaiotaomicron for 14 percent, bacteroides vulgatus and bacteroides ovatus for seven percent each, bacteroides distasonis for six percent and bacteroides uniformis for two percent. all members of the group induced bac ...	1989	2679351
role of amino acids in modulating glucose-induced desensitization of the glucose transport system.	amino acids were found to play an integral role in modulating glucose-induced desensitization of the glucose transport system (gts). when adipocytes were treated for 6 h in a defined buffer containing 25 ng/ml insulin, 20 mm glucose, plus the 15 amino acids found in dulbecco's modified eagle's medium, we observed marked desensitization of the gts, manifested by a 60% decrease in maximal insulin responsiveness (mir) and a 2.5-fold reduction in insulin sensitivity. in contrast, little or no desens ...	1989	2687266
in vitro susceptibility of feline herpesvirus-1 to vidarabine, idoxuridine, trifluridine, acyclovir, or bromovinyldeoxyuridine.	in vitro activities of 9-[( 2-hydroxyethoxy] methyl) guanine (acyclovir), (e)-5-(2-bromovinyl)-2'deoxyuridine, 9-beta-d-arabinofuranosyladenine (vidarabine), 5-iodo-2'-deoxyuridine (idoxuridine), and 5-trifluoromethyl-2'-deoxyuridine (trifluridine) were studied against 6 strains of feline herpesvirus-1. a significant difference was not detected among viral strains in their susceptibility to these compounds (p = 0.442). the relative potency of these compounds was trifluridine much greater than id ...	1989	2493210
antibody responses and protection in mice immunized with herpes simplex virus type 1 antigen immune-stimulating complex preparations.	the formation of immune-stimulating complexes (iscoms) obtained by mixing the glycoside quil a with an antigen preparation derived from herpes simplex virus type 1 (hsv-1)-infected cell cultures using a zwitterionic detergent is described. the hsv-1 antigen preparation incorporated into iscoms elicited significantly greater antibody responses in mice than the same preparation administered together with aluminium hydroxide gel, and provided complete protection against hsv-1 or hsv-2 lethal, syste ...	1989	2769234
[anaerobic bacteria in chronic renal insufficiency: apropos of a case].	we relate a case of chronic renal insufficiency complicated by urinary anaerobic infection caused by bacteroides vulgatus and peptococcus asaccharolyticus.	1989	2489402
dual-column immunoassays using protein g affinity chromatography.	tandem protein g affinity and reversed-phase chromatography (rpc) columns, coupled with a switching valve, were used for on-line immunoassays of antibodies and antigens. columns with reversibly immobilized antibodies were prepared by adsorbing antibodies on the protein g column. following antigen capture in the immunoaffinity column, antigen-antibody complexes were desorbed, dissociated, and transferred to the rpc column where they were separated and quantified. this system was used to determine ...	1989	2802148
[differentiated changes in the activity of the sympathetic nerves induced by glycopenia in spontaneously hypertensive rats].	2-deoxy-d-glucose (2-dg) evokes an increase in the efferent discharge rate of the adrenal nerve in anesthetized rats and rabbits. a 63% increase in adrenal sympathetic nerve activity (adrsna) occurred within 15 min after 125 mg/kg 2-dg administration in alert animals, no changes being revealed in the renal sympathetic nerve activity (rensna), blood pressure (bp) or the heart rate (hr). additional administration of 375 mg/kg 2-dg (cumulative dose 500 mg/kg) led to an over 120% increase in the adr ...	1989	2806664
[anaerobic bacteria in intracranial suppuration].	in four cases of intracranial suppurative disease, studied in the laboratory of anaerobic bacteria of institute pasteur of algiers, three cases are caused by anaerobic bacteria and one remain sterile. two of them are empyema with unknown origin and the others are brain abscess with bilateral chronic ear infection to one. in the three cases, gram negative anaerobic bacilli were grown in culture: bacteroides asaccharolyticus, bacteroides vulgatus and bacteroides ruminicola brevis. in two cases, th ...	1989	2489404
effects of serum testosterone level with buserelin on the activities of drug and testosterone hydroxylase and on the content of a male-specific form of cytochrome p-450 in male rats.	the effects of administration of buserelin, a synthetic agonist of lutenizing hormone-releasing hormone (lh-rh), on the content of p-450-male, a male specific form of cytochrome p-450, and the activities of drug and testosterone hydroxylases were examined in liver microsomes of male rats. administration of buserelin resulted in a decrease in the serum level of testosterone in a dose-dependent manner. similar decreases were seen in the activities of aminopyrine n-demethylase, 7-propoxycoumarin o- ...	1989	2492807
polymicrobial septicemia with clostridium difficile in acute diverticulitis.	a case is reported of a patient without previous gastrointestinal symptoms, who presented with polymicrobial septicemia caused by escherichia coli, enterococcus faecalis, clostridium difficile and bacteroides vulgatus. septicemia occurred during acute diverticulitis. a strain of clostridium difficile, which was of the same serogroup c as the blood culture isolate and also produced toxin, was recovered from the stools, but the pathogenic role of this organism in the gastrointestinal symptomatolog ...	1989	2497002
selection of constitutively resistant mutants of inducible clindamycin-resistant bacteroides vulgatus.	the rate of spontaneous mutation to constitutive clindamycin resistance and the kinetics of selection of such mutants in preinduced and non-preinduced cells was evaluated in the bacteroides vulgatus strain ryc18f6 (original mic less than or equal to 0.25 micrograms/ml), which shows inducible resistance to clindamycin and erythromycin. mutants demonstrating a high level of constitutive resistance to clindamycin (64 micrograms/ml) occurred at a frequency of 10(-7). culture in broth containing eith ...	1989	2506038
evidence that sindbis virus nsp2 is an autoprotease which processes the virus nonstructural polyprotein.	the four nonstructural proteins (nsp1-4) of sindbis virus, a member of the togaviridae family, are initially expressed from the 5' segment of the single-stranded genomic (+)rna as a polyprotein which is subsequently proteolytically processed. in attempts to identify the protease acting on this nonstructural polyprotein, we established a coupled in polyprotein, we established a coupled in vitro transcription-translation system which was able to faithfully process the major polyprotein when an mrn ...	1989	2525839
population of salmonella typhimurium in the cecum of gnotobiotic chickens.	to test the interaction of various species of bacteria with salmonella typhimurium, levels of s. typhimurium were measured in the cecum of gnotobiotic chickens inoculated with various test bacteria and subsequently with s. typhimurium. the population of s. typhimurium was suppressed in the cecum of chickens previously inoculated with escherichia coli. populations of bacteroides vulgatus, clostridium perfringens, bifidobacterium thermophilum, or lactobacillus acidophilus were transiently, though ...	1989	2539590
bacteriotherapy for chronic relapsing clostridium difficile diarrhoea in six patients.	six patients with chronic relapsing diarrhoea caused by clostridium difficile were treated with rectal instillation of homologous faeces (one patient) or a mixture of ten different facultatively aerobic and anaerobic bacteria diluted in sterile saline (five patients). the mixture led to a prompt loss of cl difficile and its toxin from the stools and to bowel colonisation by bacteroides sp, which had not been present in pre-treatment stool samples. strains of escherichia coli, cl bifermentans, an ...	1989	2566734
design, expression, and crystallization of recombinant lectin from the garden pea (pisum sativum).	the propeptide form of the lectin from the garden pea (pisum sativum agglutinin) has been expressed in escherichia coli by attaching its cdna to an inducible promoter. by a number of criteria, including the ability to form dimers, hemagglutination titer, western blot, and enzyme-linked immunosorbent assay, the resulting propeptide molecule is virtually indistinguishable from the mature proteolytically processed lectin isolated from peas. preliminary crystallization experiments using the recombin ...	1989	2708344
acetylcholine causes nicotinic depolarization in rat dorsal motor nucleus of the vagus, in vitro.	action of acetylcholine (ach) on the electrophysiologically identified preganglionic neurons in the dorsal motor nucleus of the vagus (dmv) were investigated in slice preparations of rat medulla oblongata by using conventional intracellular recording technique. applications of ach to the dmv neurons resulted in a marked depolarization, which was not abolished in ca-free superfusing solution, indicating the direct action of ach on the cells. this depolarization was associated with an increase in ...	1989	2575433
omental autoimplantation of splenic tissue and intravenous pneumococcal challenge. a comparative study in rats.	splenectomized subjects show a higher incidence of myocardial ischemia and of overwhelming post-splenectomy infection (opsi). it is doubtful that implanted splenic tissue guarantees an adequate protection from opsi. the histological characteristics and the capacity of protection from opsi of two models of omental autoimplantation of splenic tissue in rats were examined. the implanted splenic tissue offers a significant protection from pneumococcal sepsis, even though there is no relation between ...	1989	2628385
cell type-specific expression of the human transferrin gene. role of promoter, negative, and enhancer elements.	transferrin (tf), the iron transport protein of vertebrate serum, is mainly synthesized in the liver. cis-acting dna elements required for liver-specific expression of the human tf gene were identified by transient and stable expression assays in human hepatoma (hepg2 and hep3b) and epithelial carcinoma (hela) cell lines. deletion analysis of the 5' dna sequences of the gene have defined four functionally different regions: (a) a cell type-specific promoter located between positions -125 and -45 ...	1989	2708361
use of the glucose oxidase/peroxidase method for glucose assay leads to overestimation of the inhibition of gluconeogenesis by aminopyrine.	4-aminoantipyrine strongly inhibits glucose determination by the glucose oxidase/peroxidase/dianisidine assay but does not interfere in the assay using glucose-6-phosphate dehydrogenase, hexokinase and atp. as a result, the inhibition of gluconeogenesis by aminopyrine reported to be 50-90% (bánhegyi, g., mandl, j., antoni, f. and garzó, t. (1987) biochim. biophys. acta 927, 406-416) is strongly overestimated and amounts to only 10-30%.	1989	2713417
comparative in vitro activity of cefoxitin, cefotaxime alone, and in combination with desacetylcefotaxime against the bacteroides species.	the agar dilution method was used to determine the inhibitory activity of cefotaxime (ctx) alone, desacetylcefotaxime (dctx) alone, ctx plus dctx, and cefoxitin against 74 clinical isolates of the bacteroides species recovered from diabetic patients with foot ulcers. the study concluded that the addition of dctx to ctx increased the inhibitory activity from 45% to 73% for all strains tested and from 50% to 81% among the 32 strains of bacteroides fragilis. this synergistic interaction against b. ...	1989	2714070
degradation of intestinal glycoproteins by bacteroides vulgatus.	bacteroides vulgatus, isolated from a patient with crohn's disease, produced in gnotobiotic rats 7 constitutive enzymes that might be concerned with the degradation of intestinal glycoproteins. furthermore bacteroides vulgatus caused an almost complete loss of blood group antigenicity of the intestinal glycoproteins. enzymes with the potency to release toxic compounds from hepatic conjugates and plant glycosides, beta-glucuronidase and beta-glucosidase, respectively, were only detectable in smal ...	1989	2721924
transferable 5-nitroimidazole resistance in the bacteroides fragilis group.	we report the characterization of a strain of bacteroides vulgatus, bv17, that exhibits a moderate resistance to 5-nitroimidazoles and carries plasmids of 4.5, 5, 7.7, and 56 kb. a genetic determinant involved in this resistance is carried by the 7.7 +/- 0.2-kb plasmid (pip417). this plasmid can be introduced and replicated in a sensitive strain of b. fragilis 638r by transformation or by conjugation. in the latter case, the transfer may involve mobilization by the 56-kb conjugative plasmid (pip ...	1989	2740453
identification by c-banding of two human prostate tumour cell lines, 1013l and du 145.	two human prostate carcinoma cell lines are easily distinguished by c-banding. du 145 has one metacentric chromosome with a large centromeric band, 1 metacentric and 2 acrocentric chromosomes with interstitial c-bands. the y chromosome was present in 48% and 42% of the cells at passages 83 and 106, respectively, whereas in 1013l only one metacentric chromosome contained distinct extra c bands--one at the centromere and one interstitially. the y chromosome was present in 72% of the cells at passa ...	1989	2744886
[ultrastructure of human adrenal glands at various periods of prenatal morphogenesis].	as demonstrates the electron microscopical investigation of the adrenals in human embryos and fetuses 6--32-week-old, at the age of 10--12 weeks glomerular and fasciculo-reticular zones are already differentiating. subsequently, during following stages of the prenatal morphogenesis, when ultramicroscopical signs of the zonal belonging of cells and specific activity of steroid-synthesizing organells, the cortical reaction to the birth stress is manifested. installation of the cells of the medulla ...	1989	2764742
micropuncture localization of the natriuretic effect of interleukin 1.	interleukin 1 (il-1) has been demonstrated to elicit an increase in renal sodium excretion. this effect occurs in the absence of any increase in the filtered load of sodium, raising the possibility of an il-1-mediated decrease in tubule sodium reabsorption. to localize the nephron segment(s) responsible for the natriuretic effect of il-1, we performed micropuncture experiments on rats. intravenous il-1 administration caused a marked increase in sodium excretion that was not accompanied by change ...	1989	2785767
protective role of serum antibody in immunity to chlamydial genital infection.	female guinea pigs were injected intraperitoneally with pooled immunoglobulin derived from animals immunized to the chlamydial agent of guinea pig inclusion conjunctivitis. genital infections in animals receiving pooled immunoglobulin from immune animals were markedly decreased with regard to the number of inclusions detected compared with control animals. these data indicated that serum-derived antibody was able to provide a degree of protection against a chlamydial genital tract infection.	1989	2909492
protein kinase c is activated in glomeruli from streptozotocin diabetic rats. possible mediation by glucose.	glomerular inositol content and the turnover of polyphosphoinositides was reduced by 58% in 1-2 wk streptozotocin diabetic rats. addition of inositol to the incubation medium increased polyphosphoinositide turnover in glomeruli from diabetic rats to control values. despite the reduction in inositol content and polyphosphoinositide turnover, protein kinase c was activated in glomeruli from diabetic rats, as assessed by an increase in the percentage of enzyme activity associated with the particula ...	1989	2708528
activity of trospectomycin against bacteroides fragilis and other bacteroides species.	the in vitro activity of trospectomycin (u-63366; 6'-n-propyl spectinomycin pentahydrate sulfate) was evaluated against 189 clinical isolates of the bacteroides fragilis group and 65 bacteroides species isolates. at less than or equal to 8 micrograms/ml, the activity of trospectomycin compared favorably with those of clindamycin and cefoxitin against b. fragilis, bacteroides distasonis, and bacteroides vulgatus, and there was no cross resistance to these three drugs among the strains of the b. f ...	1988	2967671
the effect of oral retinoid therapy on the normal human immune system.	twenty four patients were studied prior to and after 6 and 12 weeks therapy with isotretinoin (17 patients) for acne and related disorders, or with etretinate (7 patients) for psoriasis and related disorders. patients treated with isotretinoin had a significant reduction in natural killer cell activity at an effector: target cell ratio of 100: 1 at 12 weeks and also a reduction in natural killer cell numbers at this time. patients treated with etretinate had elevated natural killer cell activity ...	1988	2972309
oestrus phases of the syrian hamster.		1988	2976222
role of intestinal anaerobic bacteria in colonization resistance.	the purpose of this study was to clarify the role of the intestinal anaerobe bacteria in colonization resistance. germfree mice were associated with escherichia coli c25 and either (a) no other species; (b) enterococcus; (c) escherichia coli m14 and proteus mirabilis, or (d) bacteroides fragilis and bacteroides vulgatus. all species colonized the cecum in high numbers, but only enterococcus significantly limited the translocation of escherichia coli c25 to mesenteric lymph nodes. however, the ov ...	1988	3132368
the red nucleus activity in rats deprived of the inferior olivary complex.	the long-term effects of the inferior olive destruction on the red nucleus activity, were studied in the rat following injection of 3-acetylpyridine. as soon as the olivary activity was suppressed, the discharge of the rubral units drastically decreased. then, they progressively recovered the control frequency during the first month, although a normal rubral activity was not restored up to 8 months. the hypothesis is advanced that the olivocerebellar system is essential to shape the activity of ...	1988	2838039
[role of alpha-1 and alpha-2-adrenoreceptors in achieving the compensatory response of the hemostasis system to plasmin].		1988	2849566
antiviral chemoprophylaxis for cytomegalovirus infection.		1988	2849602
insulin-like growth factor i binding in hepatocytes from human liver, human hepatoma, and normal, regenerating, and fetal rat liver.	insulin-like growth factor-i (igf-i) in human hepatoma cells (hep-g2) has, in addition to its effect on cell growth, short-term metabolic effects acting through its own receptor. we have demonstrated that normal human hepatocytes, compared with hep-g2 cells, have virtually no igf-i binding sites. because the rate of growth is the major difference between the hepatoma and the normal liver, we asked if normal liver might express igf-i binding sites under physiologic growth conditions. indeed, wher ...	1988	2832449
intrathecal synthesis of antibodies to human t lymphotropic virus type i and the presence of igg oligoclonal bands in the cerebrospinal fluid of patients with endemic tropical spastic paraparesis.	tropical spastic paraparesis (tsp), a neuromyelopathy predominantly involving the pyramidal tract and commonly observed in tropical and equatorial areas, was recently found to be associated with human t lymphotropic virus type i (htlv-i). we investigated sera and cerebrospinal fluid (csf) from 19 patients with tsp who were from the caribbean area, french guiana, and africa. our results showed an elevated intra-blood-brain barrier igg synthesis rate and an elevated igg index, with an increased ht ...	1988	2897399
role of starch as a substrate for bacteroides vulgatus growing in the human colon.	bacteroides vulgatus is the numerically predominant bacteroides species in the human colonic microflora. unlike other colonic bacteroides species, b. vulgatus is not a versatile utilizer of polysaccharides. the only types of polysaccharide that support rapid growth and high growth yields by all strains are the starches amylose and amylopectin. amylase and alpha-glucosidase activities are among the highest found in a bacterial fraction obtained from human feces. this observation raised the questi ...	1988	2460027
the cpg dinucleotide and human genetic disease.	reports of single base-pair mutations within gene coding regions causing human genetic disease were collated. thirty-five per cent of mutations were found to have occurred within cpg dinucleotides. over 90% of these mutations were c----t or g----a transitions, which thus occur within coding regions at a frequency 42-fold higher than that predicted from random mutations. these findings are consistent with methylation-induced deamination of 5-methyl cytosine and suggest that methylation of dna wit ...	1988	3338800
formation of dna adducts and water-soluble metabolites of benzo[a]pyrene in human monocytes is genetically controlled.	formation of dna adducts and of water-soluble metabolites was studied in monocytes of 86 first-degree relatives of 15 families. tests were performed with blood monocytes using (g-3h)-benzo[a]pyrene as a model pro-carcinogen. variance analysis revealed significantly higher inter-familial than intra-familial variations. from these data we conclude that the formation of dna adducts is genetically controlled. therefore the enhanced formation of benzo[a]pyrene dna adducts in lung cancer patients foun ...	1988	3338869
roles of hormonal and nutritional factors in the regulation of rat liver alcohol dehydrogenase activity and ethanol elimination rate in vivo.	fasting reduced the liver alcohol dehydrogenase (adh) activity by 51% (p less than 0.001). insulin, within 2 hr, increased the adh activity found in fasted animals by 28% (p less than 0.02). insulin administration failed to stimulate the reduced adh activity in diabetic rats. however, adh activity in the diabetic-fed rats decreased by 52-54% (p less than 0.001) compared to normal-fed rats regardless of whether they were meal-fed or refed the normal chow. glucagon blocked by 15% (p less than 0.02 ...	1988	3044171
free insulin profiles during intensive treatment with biosynthetic human insulin.	24 h profiles of free insulin and glucose were determined in insulin-dependent diabetic patients on intensive insulin regimens with biosynthetic human insulin, either as continuous subcutaneous insulin infusion (csii) with mealtime bolus doses (n = 6), or intensified conventional insulin therapy (icit) with preprandial injections of regular insulin and intermediate-acting insulin at bedtime (n = 6). the free insulin profiles were similar to the normal profiles but there were some important diffe ...	1988	3044863
a novel action of activin a: stimulation of insulin secretion in rat pancreatic islets.	the present study was conducted to examine an action of activin a on insulin secretion from rat pancreatic islets. in a batch incubation system, activin a stimulated insulin secretion in a dose-dependent manner at concentrations higher than 1 nm. furthermore, activin a greatly potentiated glucose-induced insulin release. when islets were perifused with 1 nm activin a, insulin secretion was barely affected in this system. however, the insulin response to 16.7 mm glucose was greatly enhanced. both ...	1988	3052451
tryptase in rat mast cells: properties and inhibition by various inhibitors in comparison with chymase.	previous studies with trans-4-(guanidinomethyl)cyclohexanecarboxylic acid 4-tert-butylphenyl ester (gmcha-ophbut), a trypsin inhibitor, strongly suggested the involvement of a trypsin-like protease in histamine release from mast cells induced by various secretagogues (takei, m., matumoto, t., endo, k. & muramatu, m. (1988) agents and actions, in press; takei, m., matumoto, t., ito, t., endo, k. & muramatu, m.; takei, m., matumoto, t., endo, k. & muramatu, m. and takei, m., matumoto, t., urashima ...	1988	3066368
future trends in the management of peptic ulcer disease.	future trends in the short- and long-term management of peptic ulcer disease are considered. the present state of development of pharmacologic agents for the short-term healing of duodenal and gastric ulcer is impressive, and high rates of healing with rapid symptom relief can be safely achieved with several agents. the problem of ulcer recurrence has not been solved, although new work concerning the role of campylobacter pylori holds promise. the mortality of acute gastrointestinal haemorrhage ...	1988	2906462
experimental evaluation of the mitroflow pericardial heart valve prosthesis. part ii. pathologic examination.	the results of the morphologic and histopathologic examination of 37 bovine pericardial heart valve prostheses that were retrieved after experimental implantation in chacma baboons for periods of one to twelve months are presented in this study. the implanted prostheses consisted of 33 mitroflow valves, size 21 mm, that belonged to four different groups according to the method of preparation of the pericardium (process i to iv) and 4 commercially available ionescu-shiley valves, size 19 mm. all ...	1988	3421507
intravenous immune globulin in symptomatic paediatric human immunodeficiency virus infection.	seven paediatric patients with symptomatic human immunodeficiency virus (hiv) infection were prospectively studied for 6-24 months after the start of intravenous immune globulin (ivig) therapy. there was substantial clinical benefit during ivig treatment with marked reduction of febrile and infectious episodes, normalization of physical and psychomotor development, and absence of mortality. the immunologic monitoring revealed some discrete objective improvements. the results of this study favour ...	1988	3134244
structure of the human liver/bone/kidney alkaline phosphatase gene.	in man, there are multiple forms of alkaline phosphatase encoded by at least three homologous genes: placental, intestinal, and liver/bone/kidney. this report describes the characterization of the human liver/bone/kidney alkaline phosphatase locus. the gene appears to exist as a single copy in the haploid genome and is comprised of 12 exons distributed over more than 50 kilobases. in liver, kidney, saos-2 human osteosarcoma cells, and cultured fibroblasts, there is a single major start for trans ...	1988	3165380
outer membrane proteins of bacteroides fragilis and bacteroides vulgatus in relation to iron uptake and virulence.	a virulent strain b. fragilis be1 and an avirulent strain b. vulgatus be20 were grown in a culture medium with and without the addition of a synthetic chelator (bipyridyl) to induce iron limitation. cells grew more slowly under iron stress, although the growth rate of the b. vulgatus strain was more affected under these conditions than the strain of b. fragilis. the outer membrane protein profile of these strains was studied in relation to the iron concentration in the growth medium by means of ...	1988	3200164
epithelium-dependent relaxation of guinea-pig tracheal smooth muscle by carbachol.	modulation of the effect of carbachol by epithelium on tracheal smooth muscle of guinea-pig was studied by using coaxial bioassay system. the contractile response of the bioassay organ (epithelium-free tracheal smooth muscle of guinea-pig) to carbachol was well maintained along the observation period of 30 min. when the same bioassay organ was taken into epithelium-intact tracheal tube of guinea-pig, a significant relaxation followed the carbachol-induced initial contraction within 30 min which ...	1988	3233050
cell responses to biomaterials. ii: endothelial cell adhesion and growth on perfluorosulfonic acid.	we report here the use of perfluorosulfonic acid (nafion) as a substratum for the growth of bovine aortal endothelial cells. this support which can be generated in a number of forms is at least as efficient in maintaining the growth of endothelial and other cell types as tissue culture grade polystyrene (tcp) and represents an advance in this regard over polytetrafluoroethylene (teflon). the mechanism underlying the different cell attachment capacities of these three polymers is not readily rela ...	1988	3241010
postnatal brain growth and allometry in the rabbit oryctolagus cuniculus.	a new four-parameter version of pütter's growth curve (pütter, 1920) recently developed by jolicoeur and pirlot (1988) is applied to cross-sectional data on brain and body weight in 66 male rabbits (oryctolagus cuniculus) ranging up to one year in postnatal age. apart from the olfactory bulb, in which there appears to be no initial delay, the estimated initial delays range from 21.7 days (after fertilization) for the cerebellum to 29.9 days for the cerebrum, which suggests that the weight of mos ...	1988	3255724
expression of ha-ras oncogene products in human neuroblastomas and the significant correlation with a patient's prognosis.	neuroblastomas represent a spectrum of diseases categorized by histological subtypes, age of the patient, and extent of tumor (stage) at diagnosis. in this study we analyzed ha-ras p21 (protein with molecular weight of 21,000) expression immunohistochemically on 47 primary human neuroblastomas resected at diagnosis. the data demonstrate that the amount of the ha-ras product correlates with a favorable prognosis (p less than 0.001) and early stages of disease at diagnosis (p less than 0.05). thes ...	1988	3276397
expression of ha-ras oncogene products in human neuroblastomas and the significant correlation with a patient's prognosis.	neuroblastomas represent a spectrum of diseases categorized by histological subtypes, age of the patient, and extent of tumor (stage) at diagnosis. in this study we analyzed ha-ras p21 (protein with molecular weight of 21,000) expression immunohistochemically on 47 primary human neuroblastomas resected at diagnosis. the data demonstrate that the amount of the ha-ras product correlates with a favorable prognosis (p less than 0.001) and early stages of disease at diagnosis (p less than 0.05). thes ...	1988	3276397
chromosome analysis of human oocytes and embryos: does delayed fertilization increase chromosome imbalance?	thirty per cent of a sample of 120 unfertilized human oocytes carried chromosome abnormalities highly correlated with maternal age (38% in patients greater than 35, as compared with 24% in younger patients). fertilized eggs, when observed 17 h after insemination, showed in 1.6% a single pronucleus suggesting parthenogenetic activation. in 92% of the cases two pronuclei were observed and the rate of chromosome anomalies depended on the morphological aspect of the embryos. triploidy was also encou ...	1988	3350934
occurrence of two phases of return to oestrus in sows on commercial units.		1988	3376405
bacteroides vulgatus outer membrane antigens associated with carrageenan-induced colitis in guinea pigs.	previous experiments with the carrageenan model for ulcerative colitis demonstrated that the inflammatory response in guinea pigs can be enhanced by immunization with bacteroides vulgatus and subsequent feeding of this organism to experimental animals. the studies reported here show that antigens extractable from the bacterial outer membrane by edta are responsible for this effect. sodium dodecyl sulfate-polyacrylamide gel electrophoresis was used to analyze the outer membrane proteins from vari ...	1988	3384476
induction of gene amplification by arsenic.	arsenic is a well-established carcinogen in humans, but there is little evidence for its carcinogenicity in animals and it is inactive as an initiator or tumor promoter in two-stage models of carcinogenicity in mice. two arsenic salts (sodium arsenite and sodium arsenate) induced a high frequency of methotrexate-resistant 3t6 cells, which were shown to have amplified copies of the dihydrofolate reductase gene. the ability of arsenic to induce gene amplification may relate to its carcinogenic eff ...	1988	3388020
alveolar macrophage subpopulations' responsiveness to chemotactic stimuli.	recent data suggest that alveolar macrophages are a heterogeneous group of cells with several subpopulations. this study was undertaken to determine if there is heterogeneity among rat alveolar macrophage subpopulations ability to respond to chemotactic stimuli. alveolar macrophages were harvested by bronchoalveolar lavage and fractionated into density-defined fractions by centrifugation through a continuous isoosmotic gradient of colloidal silica. unfractionated and density-defined alveolar mac ...	1988	3394797
the effect of tissue culture agar on chromosome breakage, sister-chromatid exchanges and clonogenicity in human cells.	to investigate the cytogenetic effects of electromagnetic fields, a system containing an agar gel was developed to support the growth of various human cell types (peripheral lymphocytes, lymphoblasts, and fibroblasts). when compared to alioquots of identical cells, grown in plastic culture vessels, statistically significant increases in the frequencies of chromosome breakage, sister-chromatid exchange and decreased cloning efficiency were observed in those cells cultured in the agar. these resul ...	1988	3398870
high-performance liquid chromatography with on-line radioactivity monitoring for metabolic studies of 2- and 4-[14c]aminobiphenyls.		1988	3417828
recurrent hypercalcemia and elevated 1,25-dihydroxyvitamin d levels in hodgkin's disease.	hypercalcemia has been infrequently associated with hodgkin's disease. when seen, most cases have been attributable to skeletal invasion by disease. herein is described a 40-year-old man with a 15-year history of hodgkin's disease. each of four disease recurrences was heralded by hypercalcemia occurring in the absence of bone disease or elevation of parathyroid hormone levels. marked elevations of 1,25-dihydroxyvitamin d levels were observed that paralleled his disease course and response to the ...	1988	3337120
rate of incorporation of radiolabelled nucleosides does not necessarily reflect the metabolic state of cells in culture: effects of latent mycoplasma contamination.	in response to cell-free conditioned medium derived from the human bladder carcinoma line t24 (t24 sn), we found greatly reduced incorporation of tritiated thymidine and uridine ([3h]tdr, [3h]ur) by the human carcinoma lines uchncu (small-cell lung carcinoma) and ls174t (colon carcinoma). the effect was not due to an excess of nucleosides or cytokines known to be present in t24 sn. cell-cycle distribution, increase in cell numbers, and de novo nucleoside synthesis in the indicator cells were onl ...	1988	3338817
the future of community health nursing.		1988	259473
cultivation of medulloblastoma cells derived from simian adenovirus sa7-induced hamster brain tumor.	in vitro cultivation of medulloblastoma cells was successfully established from hamster brain tumors induced by simian adenovirus sa7. these tumor cells possess morphological features in cultures that are very similar to those of the original tumor. both tubular and rosette growth patterns were evident. at the ultrastructural level only small numbers of cytoplasmic organelles could be detected in the tumor cells, typical of generally immature and/or undifferentiated cells. the proliferation of t ...	1988	172225
[withouth the language one cannot practice nursing].		1987	260420
rigidity in rats due to radio frequency decerebration and effects of chlorpromazine and mephenesin.	bilateral radio frequency (rf) lesions of the midbrain were produced in an attempt to establish a non-bleeding method of producing decerebrate rigidity in rats. marked extensor rigidity occurred with a high reproducibility in hindlimbs without the appearance of voluntary movement. stretch reflex tension induced by repetitive dorsiflexion of the hindfeet was employed as a measure of the intensity of rigidity. chlorpromazine-hcl (0.1-1 mg/kg, i.v.) and mephenesin (10-50 mg/kg, i.v.) which depress ...	1987	3557053
identification in rat liver and serum of water-soluble class i mhc molecules possibly homologous to the murine q10 gene product.	we have identified large quantities of a water-soluble, non-rt1.a class i mhc molecule in the serum of the da rat strain, with a similar molecule being found in aqueous extracts of da liver. the non-rt1.a class i molecules have heavy chains of 41 kd, which is smaller than rt1.a class i molecules isolated from liver membranes (45 kd) but larger than water-soluble rt1.a class i molecules previously identified in serum and aqueous extracts of liver and kidney (40 kd). nh3-terminal amino acid sequen ...	1987	3585249
increased faecal glycosidases in patients with crohn's disease.	the strictly anaerobic faecal flora of patients with crohn's disease (cd) contains higher numbers of coccoid rods (eubacterium, peptostreptococcus and coprococcus species) and gram-negative rods (bacteroides vulgatus) than the flora of healthy subjects. the abnormal flora of patients with cd might affect levels of glycolytic enzymes and metabolic products and thus play some role in the pathogenesis of cd. therefore, the activity of 23 glycosidases that can degrade mucus glycoproteins or plant po ...	1987	3609504
[mechanism of changes in irradiated thymocytes detected by anilinonaphthalenesulfonate].	in experiments on rat thymocytes obtained 15 min, 2 h and 4 h after irradiation of animals with the dose of 10 gy it was found that 15 min and 4 h following irradiation changes occurred in their membranes increasing the lifetime of the excited state of anilinonaphthalene sulfonate and hydrofobicity and viscosity of its microenvironment. the indicated parameters did not vary from the controls 2 h following irradiation. the analysis of the data obtained prompted a suggestion that the observed chan ...	1987	3628737
abdominal operations: effect on subsequent experimental faecal peritonitis.	rats were subjected to laparotomy, anaesthesia only (controls), laparotomy plus 1-cm-segment ileum isolation, or no treatment (controls) and were challenged with endogenous faecal peritonitis 1 and 2 weeks later, respectively. survival rates were 40% for the laparotomy group and 100% for the ileum isolation group, whereas all controls died. the number of peritoneal leucocytes, particularly macrophages and neutrophils, was significantly (p less than 0.05) higher in the laparotomy group than in th ...	1987	3629184
newt epidermal cell migration in vitro and in vivo appears to involve arg-gly-asp-ser receptors.	the effect of a synthetic peptide consisting of arg-gly-asp-ser (rgds), the amino acid sequence representing the fibroblast attachment site in fibronectin (fn), was tested on migrating newt epidermal cells. in one approach, skin explants were placed on the bottom of plastic dishes coated with human fn, human fibrinogen (fgn), human serum spreading factor (sf), or bovine type i collagen. the explants were then incubated overnight in serum-free medium with or without rgds. in these experiments exp ...	1987	3654790
prevalence of hepatitis b markers in occupational health nurses.	a seroprevalence study was undertaken at the 14th annual conference of the occupational health nurses association of ontario. a total of 151 self-selected registrants (33%) provided demographic, health, and occupational information. blood was drawn for hepatitis b serology (hepatitis b surface antigen, core antibody, and surface antibody). vaccine-induced seropositivity was found in 11 persons (7.3%) while naturally occurring markers were present in 13 nurses (8.6%). this exceeds the natural pre ...	1987	3655952
serological evaluation of the outer membrane protein complexes of five saccharolytic intestinal bacteroides species.	the purpose of this investigation was to develop a serological procedure for rapid identification of the following five bacteroides species: bacteroides distasonis, bacteroides fragilis, bacteroides ovatus, bacteroides uniformis, and bacteroides vulgatus. the outer membrane fractions were assayed using sds-polyacrylamide slab gel electrophoretic techniques. the species-specific protein band from each species as well as the group-specific protein were purified and used to develop an indirect enzy ...	1987	3665895
the influence of various bacteria on the cecal mucosa of monoflora chickens infected with eimeria tenella. a scanning electron microscopic study.	monoflora chickens were established at the age of 2 days by an oral inoculation of one of six species of bacteria (lactobacillus acidophilus, bifidobacterium thermophilum, bacteroides vulgatus, clostridium perfringens, escherichia coli, or streptococcus faecalis). two days later the chickens were infected with eimeria tenella (5 x 10(4) oocytes per bird). there were four groups: uninfected birds as controls, the birds infected with either bacteria or e. tenella alone, and the birds infected with ...	1987	3673342
the binding of decomposition products of udp-galactose to the microsomes and polyribosomes isolated from rat liver.	udp-d-[u-14c]galactose is decomposed to [u-14c]galactose-1-phosphate and [u-14c]galactose by rat liver microsomal and crude polyribosomal fractions, under conditions commonly used to assay of glycosyltransferase activities. udp-d-[u-14c]galactose, at neutral ph, is also chemically degraded to the [u-14c]galactose-1,2-cyclic phosphate. the 1,2-cyclic phosphate derivative of galactose also exists in the commercial udp-d-[u-14c]galactose. it is a very important finding that products of the udp-d-[u ...	1987	3675917
multipotential differentiation of human y-79 retinoblastoma cells in attachment culture.	the human y-79 retinoblastoma cell line has been studied in attachment culture. evidence is presented of its capability to differentiate partially into cells with characteristics of photoreceptors, conventional neurons, glia and pigment epithelial cells as influenced by appropriate combinations of substrata and differentiating agents. we conclude that retinoblastoma could originate from a primitive neuroectodermal cell of multi-potential character.	1987	3568139
detoxication of cyanide in the chicken by conversion to thiocyanate, as influenced by the availability of transferable sulphur.	the urinary excretion of thiocyanate by hens after dosage with cyanide (30 mumol) has been studied in a series of acute experiments involving 6 hr urine collection periods. more than half of the dose could be recovered as thiocyanate when cyanide was given by intravenous infusion and the rate of excretion closely paralleled plasma thiocyanate concentration. little cyanide was excreted directly. the excretion of thiosulphate fell by an amount that suggested that availability of sulphane sulphur m ...	1987	3829625
effects of cysteine and antioxidants on the hepatic redox-state, acetaldehyde and triglyceride levels after acute ethanol dosing.	cysteine and the synthetic antioxidants butylated hydroxytoluene (bht) and n,n'-diphenyl-phenylenediamine (dppd) have been found to protect against the increase in hepatic triglycerides caused by acute ethanol administration (2 g/kg/i.p.) in rats. none of these agents affected the ethanol-induced increase in the hepatic redox-state, measured as lactate/pyruvate and 3-hydroxybutyrate/acetoacetate ratios, and there was no influence of any of the compounds on ethanol metabolism. of the three agents ...	1987	3426693
electrophysiological and behavioral changes after destruction of the basolateral amygdaloid nucleus in rats.		1987	3431682
value of thyroid follow-up registers.	in this study from a county in southern sweden 269 of 271 patients (99.3%) treated with radioiodine for hyperthyroidism over a two-year period were followed up five years later. the routine procedures at hospital clinics and primary health care centres were studied. a high number of toxic multinodular goitre (n = 113; 42.0%) and toxic adenoma (n = 76; 28.3%) emerged, 80 patients (29.7%) had toxic diffuse goitre (graves's disease). during the five-year follow-up the cumulative incidence of hypoth ...	1987	3671919
evaluation of the syphilis bio-enzabead assay for detection of treponemal antibody.	an enzyme-linked immunosorbent assay for treponema pallidum antibody (syphilis bio-enzabead kit; organon teknika corp., formerly litton bionetics, kensington, md.) was compared with the standard fluorescent treponemal antibody absorption test for syphilis (indirect fluorescent-antibody confirmatory test; zeus scientific inc., raritan, n.j.). six hundred specimens tested in the rapid plasma reagin card test (hynson, westcott and dunning, inc., baltimore, md.) and microhemagglutination assay for a ...	1987	3294884
menaquinone production and utilization in germ-free rats after inoculation with specific organisms.	although menaquinones are biologically active forms of vitamin k, factors that influence their production by bacteria or their absorption from the gut are not well understood. germ-free rats were inoculated with four different strains of organisms and fecal and tissue menaquinone concentrations were determined. no menaquinones were detected in the tissues or feces of rats colonized with bifidobacterium longum or clostridium ramosum, two organisms that have not been reported to produce menaquinon ...	1987	3298580
deconjugation of bile acids by human intestinal bacteria implanted in germ-free rats.	fecal bile acids in germ-free rats were analyzed after inoculation with bacteroides vulgatus, bifidobacterium longum, escherichia coli or clostridium ramosum. b. vulgatus preferentially deconjugated tauro-beta-muricholic acid and b. longum taurocholic acid. c. ramosum deconjugated both bile acids, but e. coli deconjugated neither. 7 alpha-dehydroxylation of bile acids was negligible even after 18 days of inoculation, but a small amount of 7-oxo-bile acids, less than 5%, was formed. fecal excreti ...	1987	3312906