Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| causality of chlamydiae in arthritis and spondyloarthritis: a plea for increased translational research. | current molecular genetic understanding of the metabolically active persistent infection state of chlamydia trachomatis and chlamydia pneumoniae in the synovium in patients with arthritis and spondyloarthritis favors a causal relationship. here, we examine how adequately the accepted criteria for that etiologic relationship are fulfilled, emphasizing the situation in which these microorganisms cannot be cultivated by standard or other means. we suggest that this unusual situation of causality by ... | 2016 | 26769308 |
| interaction of chlamydiae with human macrophages. | the phylum chlamydiae contains several members that are well-known human pathogens, like chlamydia trachomatis and c. pneumoniae. establishing a chronic bacterial infection requires the active evasion of the host immune response. a major arm of the innate immune defence is constituted by macrophages, which fight infections by removing bacteria and triggering an adaptive immune response. however, some pathogenic chlamydia infect and survive in macrophages at least for a certain period of time. th ... | 2016 | 26613554 |
| chlamydia trachomatis is resistant to inclusion ubiquitination and associated host defense in gamma interferon-primed human epithelial cells. | the cytokine gamma interferon (ifn-γ) induces cell-autonomous immunity to combat infections with intracellular pathogens, such as the bacterium chlamydia trachomatis the present study demonstrates that ifn-γ-primed human cells ubiquitinate and eliminate intracellular chlamydia-containing vacuoles, so-called inclusions. we previously described how ifn-γ-inducible immunity-related gtpases (irgs) employ ubiquitin systems to mark inclusions for destruction in mouse cells and, furthermore, showed tha ... | 2016 | 27965446 |
| computational modeling of tc0583 as a putative component of the chlamydia muridarum v-type atp synthase complex and assessment of its protective capabilities as a vaccine antigen. | numerous chlamydia trachomatis proteins have been identified as potential subunit vaccines, of which the major outer-membrane protein (momp) has, so far, proven the most efficacious. recently, subunit a of the v-type atp synthase (atpase; tc0582) complex was shown to elicit partial protection against infection. computational modeling of a neighboring gene revealed a novel subunit of the v-type atpase (tc0583). to determine if this newly identified subunit could induce protection and/or enhance t ... | 2016 | 26706820 |
| host nectin-1 promotes chlamydial infection in the female mouse genital tract, but is not required for infection in a novel male murine rectal infection model. | chlamydia trachomatis is the most common bacterial sexually transmitted pathogen, but more than 70% of patients fail to seek treatment due to the asymptomatic nature of these infections. women suffer from numerous complications from chronic chlamydial infections, which include pelvic inflammatory disease and infertility. we previously demonstrated in culture that host cell nectin-1 knockdown significantly reduced chlamydial titers and inclusion size. here, we sought to determine whether nectin-1 ... | 2016 | 27486990 |
| mri as a novel in vivo approach for assessing structural changes of chlamydia pathology in a mouse model. | chlamydia trachomatis is among the most prevalent of sexually transmitted diseases. while chlamydia infection is a reportable event and screening has increased over time, enhanced surveillance has not resulted in a reduction in the rate of infections, and chlamydia infections frequently recur. the development of a preventative vaccine for chlamydia may be the only effective approach for reducing infection and the frequency of pathological outcomes. current vaccine research efforts involve time c ... | 2016 | 27467585 |
| iga modulates respiratory dysfunction as a sequela to pulmonary chlamydial infection as neonates. | neonatal chlamydia lung infections are associated with serious sequelae such as asthma and airway hyper-reactivity in children and adults. our previous studies demonstrated the importance of th-1 type cytokines, il-12 and ifn-γ in protection against neonatal pulmonary chlamydial challenge; however, the role of the humoral arm of defense has not been elucidated. we hypothesized that b-cells and iga, the major mucosal antibody, play a protective role in newborns against development of later life r ... | 2016 | 26755533 |
| a prospective observational study of vulvovagintis in pregnant women in argentina, with special reference to candidiasis. | to evaluate the frequency of yeast, bacteria or protozoa in pregnant women and to correlate the possible associations of these microorganisms and their relationships with vulvovaginitis (vv) and cervicitis. vaginal specimens were collected and prepared for smears in microscope slides for the evaluation of yeast, trichomonas vaginalis and bacteria. samples were cultured in specific culture medium. cervical specimens were used to investigate the presence of neisseria gonorrhoeae, chlamydia trachom ... | 2016 | 26931504 |
| natural products for the treatment of chlamydiaceae infections. | due to the global prevalence of chlamydiae, exploring studies of diverse antichlamydial compounds is important in the development of effective treatment strategies and global infectious disease management. chlamydiaceae is the most widely known bacterial family of the chlamydiae order. among the species in the family chlamydiaceae, chlamydia trachomatis and chlamydia pneumoniae cause common human diseases, while chlamydia abortus, chlamydia psittaci, and chlamydia suis represent zoonotic threats ... | 2016 | 27754466 |
| analysis of polymorphic membrane protein expression in cultured cells identifies pmpa and pmph of chlamydia psittaci as candidate factors in pathogenesis and immunity to infection. | the polymorphic membrane protein (pmp) paralogous families of chlamydia trachomatis, chlamydia pneumoniae and chlamydia abortus are putative targets for chlamydia vaccine development. to determine whether this is also the case for pmp family members of c. psittaci, we analyzed transcription levels, protein production and localization of several pmps of c. psittaci. pmp expression profiles were characterized using quantitative real-time pcr (rt-qpcr), immunofluorescence (if) and immuno-electron m ... | 2016 | 27631978 |
| the presence of chlamydia phage phicpg1 capsid protein vp1 genes and antibodies in patients infected with chlamydia trachomatis. | chlamydia phage phicpg1 has been found in chlamydia caviae in a guinea pig model for inclusion conjunctivitis, raising the possibility that chlamydia phage is also present in patients infected with c. trachomatis (ct). in the present study, we assayed for presence of chlamydia phage capsid protein vp1 genes and antibodies in 84 non-ct controls and 206 ct patients using an enzyme-linked immunoassay (elisa), followed by verification with western blot. none of the subjects were exposed to an antibi ... | 2016 | 27213552 |
| chlamydiaphage φcpg1 capsid protein vp1 inhibits chlamydia trachomatis growth via the mitogen-activated protein kinase pathway. | chlamydia trachomatis is the most common cause of curable bacterial sexually transmitted infections worldwide. although the pathogen is well established, the pathogenic mechanisms remain unclear. given the current challenges of antibiotic resistance and blocked processes of vaccine development, the use of a specific chlamydiaphage may be a new treatment solution. φcpg1 is a lytic phage specific for chlamydia caviae, and shows over 90% nucleotide sequence identity with other chlamydiaphages. vp1 ... | 2016 | 27089359 |
| biodegradable polymeric nanoparticles-based vaccine adjuvants for lymph nodes targeting. | vaccines have successfully eradicated a large number of diseases. however, some infectious diseases (such as hiv, chlamydia trachomatis or bacillus anthracis) keep spreading since there is no vaccine to prevent them. one way to overcome this issue is the development of new adjuvant formulations which are able to induce the appropriate immune response without sacrificing safety. lymph nodes are the site of lymphocyte priming by antigen-presenting cells and subsequent adaptive immune response, and ... | 2016 | 27754314 |
| screening for abnormal vaginal microflora by self-assessed vaginal ph does not enable detection of sexually transmitted infections in ugandan women. | is self-assessed vaginal ph measurement to detect abnormal vaginal bacterial microflora (avf) an adequate prescreening method for detection of genital sexually transmitted infections (stis)? | 2016 | 27112831 |
| study of the prevalence and association of ocular chlamydial conjunctivitis in women with genital infection by chlamydia trachomatis, mycoplasma genitalium and candida albicans attending outpatient clinic. | to determine the association between chlamydial conjunctivitis and genital infection by chlamydia trachomatis, mycoplasma genitalium and candida albicans, in addition to the possible relationship between cultured bacterial pathogens and oculogenital chlamydial infection. | 2016 | 27588273 |
| recombinant human peptidoglycan recognition proteins reveal antichlamydial activity. | peptidoglycan recognition proteins (pglyrps) are innate immune components that recognize the peptidoglycan and lipopolysaccharides of bacteria and exhibit antibacterial activity. recently, the obligate intracellular parasite chlamydia trachomatis was shown to have peptidoglycan. however, the antichlamydial activity of pglyrps has not yet been demonstrated. the aim of our study was to test whether pglyrps exhibit antibacterial activity against c. trachomatis thus, we cloned the regions containing ... | 2016 | 27160295 |
| lipopolysaccharide (lps) disrupts particle transport, cilia function and sperm motility in an ex vivo oviduct model. | the oviduct functions in the transportation of gametes to the site of fertilization (the ampulla) and is the site of early embryonic development. alterations of this early developmental environment, such as the presence of sexually transmitted pathogens, may affect oviduct function leading to reduced fertilization rates and contribute to compromised embryonic development. in this study, sperm interactions, particle transport speed (pts) and cilia beat frequency (cbf) in the ampulla following exp ... | 2016 | 27079521 |
| recombinant expression of chlamydia trachomatis major outer membrane protein in e. coli outer membrane as a substrate for vaccine research. | chlamydia trachomatis is a human pathogen which causes a number of pathologies, including genital tract infections in women that can result in tubal infertility. prevention of infection and disease control might be achieved through vaccination; however, a safe, efficacious and cost-effective vaccine against c. trachomatis infection remains an unmet medical need. c. trachomatis major outer membrane protein (momp), a β-barrel integral outer membrane protein, is the most abundant antigen in the out ... | 2016 | 27464881 |
| patterns of extragenital chlamydia and gonorrhea in women and men who have sex with men reporting a history of receptive anal intercourse. | screening for chlamydia trachomatis (ct) and neisseria gonorrhoeae (gc) in men who have sex with men is risk based. despite high frequencies of oral and receptive anal intercourse (rai) among women, extragenital screening is not recommended. | 2016 | 26766527 |
| simultaneous detection of multiple lower genital tract pathogens by an impedimetric immunochip. | lower genital tract infections caused by both sexually and not-sexually transmitted pathogens in women are a key public health priority worldwide, especially in developing countries. since standard analyses are time-consuming, appropriate therapeutic intervention is often neglected or delayed. lab-on-chips and biosensors open new perspectives and offer innovative tools to simplify the diagnosis by medical staff, especially in countries with inadequate resources. here we report a biosensing platf ... | 2016 | 26686917 |
| gene deletion by fluorescence-reported allelic exchange mutagenesis in chlamydia trachomatis. | although progress in chlamydia genetics has been rapid, genomic modification has previously been limited to point mutations and group ii intron insertions which truncate protein products. the bacterium has thus far been intractable to gene deletion or more-complex genomic integrations such as allelic exchange. herein, we present a novel suicide vector dependent on inducible expression of a chlamydial gene that renders chlamydia trachomatis fully genetically tractable and permits rapid reverse ge ... | 2016 | 26787828 |
| ire1α mediates pkr activation in response to chlamydia trachomatis infection. | protein kinase rna activated (pkr) is a crucial mediator of anti-viral responses but is reported to be activated by multiple non-viral stimuli. however, mechanisms underlying pkr activation, particularly in response to bacterial infection, remain poorly understood. we have investigated mechanisms of pkr activation in human primary monocyte-derived dendritic cells in response to infection by chlamydia trachomatis. infection resulted in potent activation of pkr that was dependent on tlr4 and myd88 ... | 2016 | 27021640 |
| latent infections as a risk factor for posttrabeculectomy bleb failure. | to investigate latent conjunctival chlamydia trachomatis (ct) and bacteroides fragilis (bf) infections as potential risk factors for posttrabeculectomy bleb failure. | 2016 | 25651208 |
| prevalence of chlamydia trachomatis and neisseria gonorrhoeae infection in adolescents in northern italy: an observational school-based study. | we carried out a study to evaluate the prevalence of chlamydia trachomatis and neisseria gonorrhoeae genital infections in school-based adolescents in northern italy. | 2016 | 26927226 |
| quantifying promoter activity during the developmental cycle of chlamydia trachomatis. | chlamydia trachomatis is an important human pathogen that undergoes a characteristic development cycle correlating with stage-specific gene expression profiles. taking advantage of recent developments in the genetic transformation in c. trachomatis, we constructed a versatile green fluorescent protein (gfp) reporter system to study the development-dependent function of c. trachomatis promoters in an attempt to elucidate the mechanism that controls c. trachomatis adaptability. we validated the us ... | 2016 | 27263495 |
| in vitro activity of quaternary ammonium surfactants against streptococcal, chlamydial, and gonococcal infective agents. | quaternary ammonium compounds (qac) are widely used, cheap, and chemically stable disinfectants and topical antiseptics with wide-spectrum antimicrobial activities. within this group of compounds, we recently showed that there are significant differences between the pharmacodynamics of n-alkyl quaternary ammonium surfactants (qas) with a short (c12) alkyl chain when in vitro toxicities toward bacterial and mammalian epithelial cells are compared. these differences result in an attractive therape ... | 2016 | 26976875 |
| high prevalence of chlamydia trachomatis and neisseria gonorrhoeae infections in anal and pharyngeal sites among a community-based sample of men who have sex with men and transgender women in lima, peru. | this study aimed to characterise the epidemiology of chlamydia trachomatis (ct) and neisseria gonorrhoeae (ng) infections among men who have sex with men (msm) and transgender women (tw) in lima, peru. | 2016 | 26739719 |
| performance evaluation of the pelvocheck ct/ng test kit for the detection of chlamydia trachomatis and neisseria gonorrhoeae. | assessment of the performance of the pelvocheck ct/ng test (greiner-bio-one gmbh) to detect chlamydia trachomatis (ct) and neisseria gonorrhoeae (ng) in first-void urine (fvu) of females. | 2016 | 26729391 |
| genital chlamydia trachomatis infection among women of reproductive age attending the gynecology clinic of hawassa university referral hospital, southern ethiopia. | urogenital infection with chlamydia trachomatis(ct) is one of the most common bacterial sexually transmitted infections (stis) world-wide, especially in developing nations where routine laboratory diagnosis is unavailable. little is known about the epidemiology of this infection in ethiopia where other stis are prevalent. this study was conducted to determine the prevalence and associated factors of ct infection among women of reproductive age. | 2016 | 28006003 |
| influence of storage time on dna of chlamydia trachomatis, ureaplasma urealyticum, and neisseria gonorrhoeae for accurate detection by quantitative real-time polymerase chain reaction. | the shipment and storage conditions of clinical samples pose a major challenge to the detection accuracy of chlamydia trachomatis (ct), neisseria gonorrhoeae (ng), and ureaplasma urealyticum (uu) when using quantitative real-time polymerase chain reaction (qrt-pcr). the aim of the present study was to explore the influence of storage time at 4°c on the dna of these pathogens and its effect on their detection by qrt-pcr. ct, ng, and uu positive genital swabs from 70 patients were collected, and d ... | 2016 | 27580005 |
| mycoplasma genitalium prevalence, coinfection, and macrolide antibiotic resistance frequency in a multicenter clinical study cohort in the united states. | the prevalence rates of mycoplasma genitalium infections and coinfections with other sexually transmitted organisms and the frequency of a macrolide antibiotic resistance phenotype were determined in urogenital specimens collected from female and male subjects enrolled in a multicenter clinical study in the united states. specimens from 946 subjects seeking care from seven geographically diverse clinical sites were tested for m. genitalium and for chlamydia trachomatis, neisseria gonorrhoeae, an ... | 2016 | 27307460 |
| diagnostic procedures to detect chlamydia trachomatis infections. | the intracellular life style of chlamydia and the ability to cause persistent infections with low-grade replication requires tests with high analytical sensitivity to directly detect c. trachomatis (ct) in medical samples. nucleic acid amplification tests (naats) are the most sensitive assays with a specificity similar to cell culture and are considered the method of choice for ct detection. in addition, naats can be performed on various clinical specimens that do not depend on specific transpor ... | 2016 | 27681919 |
| recruitment of minority adolescents and young adults into randomised clinical trials: testing the design of the technology enhanced community health nursing (tech-n) pelvic inflammatory disease trial. | pelvic inflammatory disease (pid) disproportionately affects adolescent and young adult (aya) women and can negatively influence reproductive health trajectories. few randomized controlled trials (rcts) have focused on strategies to improve outpatient adherence or to reduce reproductive morbidity in this population. this paper describes the research methods and preliminary effectiveness of recruitment, retention, and intervention strategies employed in a novel rct designed to test a technology-e ... | 2016 | 27617108 |
| a systematic review of point of care testing for chlamydia trachomatis, neisseria gonorrhoeae, and trichomonas vaginalis. | objectives. systematic review of point of care (poc) diagnostic tests for sexually transmitted infections: chlamydia trachomatis (ct), neisseria gonorrhoeae (ng), and trichomonas vaginalis (tv). methods. literature search on pubmed for articles from january 2010 to august 2015, including original research in english on poc diagnostics for sexually transmitted ct, ng, and/or tv. results. we identified 33 publications with original research on poc diagnostics for ct, ng, and/or tv. thirteen articl ... | 2016 | 27313440 |
| gonorrhoea diagnostic and treatment uncertainties: risk factors for culture negative confirmation after positive nucleic acid amplification tests. | gonorrhoea incidence has increased substantially in stockholm during the past years. these increases have coincided with changes in testing practice from solely culture-based to nucleic acid amplification tests (naat). gonorrhoea naat is integrated with chlamydia trachomatis testing and due to opportunistic screening for chlamydia, testing prevalence for gonorrhoea has increased substantially in the stockholm population. the aim of this study was to examine epidemiological risk-factors for disco ... | 2016 | 27152704 |
| comparative analysis of syndromic and pcr-based diagnostic assay reveals misdiagnosis/ overtreatment for trichomoniasis based on subjective judgment in symptomatic patients. | trichomoniasis, a sexually transmitted disease (std), is caused by trichomonas vaginalis in both men and women. screening of trichomoniasis is problematic in resource challenged settings as currently available, inexpensive diagnostic methods are of low sensitivity and/or specificity. in india, national aids control organization (naco) recommended syndromic case management (scm) for treatment. the objective of the present study was to compare the utility of the naco-nacp iii algorithms for sti/rt ... | 2016 | 27146362 |
| prevalence and risk factors of three curable sexually transmitted infections among women in nairobi, kenya. | sexually transmitted infections (stis) are a major public health problem, especially in developing countries. the complications of untreated stis in the female genital tract and their role in adverse pregnancy and perinatal outcomes have been well documented. the prevalence of stis in kenya among women in the general population has not been extensively studied and there is a lack of guidelines for screening of non-pregnant women. knowledge of the prevalence of curable stis among this population ... | 2016 | 27026347 |
| acceptability and feasibility of sexually transmitted infection testing and treatment among pregnant women in gaborone, botswana, 2015. | chlamydia trachomatis (ct), neisseria gonorrhoeae (ng), and trichomonas vaginalis (tv) are curable sexually transmitted infections (stis) that can cause adverse maternal and birth outcomes. most countries do not conduct routine testing during antenatal care. we present data on the acceptability and feasibility of testing and treating pregnant women for stis in an antenatal clinic in gaborone, botswana. | 2016 | 27119076 |
| sequestration of host metabolism by an intracellular pathogen. | for intracellular pathogens, residence in a vacuole provides a shelter against cytosolic host defense to the cost of limited access to nutrients. the human pathogen chlamydia trachomatis grows in a glycogen-rich vacuole. how this large polymer accumulates there is unknown. we reveal that host glycogen stores shift to the vacuole through two pathways: bulk uptake from the cytoplasmic pool, and de novo synthesis. we provide evidence that bacterial glycogen metabolism enzymes are secreted into the ... | 2016 | 26981769 |
| prevalence of trichomonas vaginalis in women visiting 2 obstetrics and gynecology clinics in daegu, south korea. | this study explored epidemiological trends in trichomoniasis in daegu, south korea. wet mount microscopy, pcr, and multiplex pcr were used to test for trichomonas vaginalis in vaginal swab samples obtained from 621 women visiting 2 clinics in daegu. of the 621 women tested, microscopy detected t. vaginalis in 4 (0.6%) patients, pcr detected t. vaginalis in 19 (3.0%) patients, and multiplex pcr detected t. vaginalis in 12 (1.9%) patients. testing via pcr demonstrated high sensitivity and high neg ... | 2016 | 26951983 |
| the "3 in 1" study: pooling self-taken pharyngeal, urethral, and rectal samples into a single sample for analysis for detection of neisseria gonorrhoeae and chlamydia trachomatis in men who have sex with men. | triple-site testing (using pharyngeal, rectal, and urethral/first-void urine samples) for neisseria gonorrhoeae and chlamydia trachomatis using nucleic acid amplification tests detects greater numbers of infections among men who have sex with men (msm). however, triple-site testing represents a cost pressure for services. msm over 18 years of age were eligible if they requested testing for sexually transmitted infections (stis), reported recent sexual contact with either c. trachomatis or n. gon ... | 2016 | 26719439 |
| mass drug administration of azithromycin for trachoma reduces the prevalence of genital chlamydia trachomatis infection in the solomon islands. | chlamydia trachomatis is the most common bacterial sexually transmitted infection and is frequently asymptomatic; ocular c. trachomatis strains cause trachoma. mass drug administration (mda) of azithromycin for trachoma might also reduce the prevalence of genital c. trachomatis. in a survey conducted in the solomon islands in 2014, prior to mda, the prevalence of genital c. trachomatis was 20.3% (95% ci 15.9% to 25.4%). we conducted a survey to establish the impact of mda with azithromycin on ge ... | 2016 | 26888658 |
| sexually transmitted infections and use of contraceptives in women living with hiv in denmark - the shade cohort. | no danish guidelines for screening of sexually transmitted infections (stis) in women living with hiv (wlwh) exist, except for annual syphilis testing. drug-drug interaction between hormonal contraceptives and some types of highly active antiretroviral therapy (haart) occurs. we assessed prevalence of stis, contraceptive choices and predictors of condom use in a cohort of wlwh in denmark. | 2016 | 26880101 |
| clinical laboratory assessment of mycoplasma genitalium transcription-mediated amplification using primary female urogenital specimens. | following analysis of primary cervix, vagina, and first-void female urine specimens for chlamydia trachomatis, neisseria gonorrhoeae, and trichomonas vaginalis via commercial transcription-mediated amplification (tma), residual material was subjected to mycoplasma genitalium research-use-only tma. representation within a 2,478-specimen retrospective study set was established by comparison to a 6-month audit of clinical c. trachomatis tma (12,999 specimens) on the basis of the c. trachomatis dete ... | 2016 | 26659209 |
| prevalence of reproductive tract infections and the predictive value of girls' symptom-based reporting: findings from a cross-sectional survey in rural western kenya. | reproductive tract infections (rtis), including sexually acquired, among adolescent girls is a public health concern, but few studies have measured prevalence in low-middle-income countries. the objective of this study was to examine prevalence in rural schoolgirls in kenya against their reported symptoms. | 2016 | 26819339 |
| sti tests and proportion of positive tests in female sex workers attending local public health departments in germany in 2010/11. | in germany, local public health departments (lphd) are required to offer low-threshold access to confidential counselling and testing for sexually transmitted infections (sti) for sex workers. we collected data from lphd in germany to estimate the number of performed sti tests and the proportion of positive sti tests among attending female sex workers (fsw) in order to formulate recommendations for improving sti testing and care for fsw in germany. | 2016 | 27871264 |
| high prevalence of hiv, chlamydia and gonorrhoea among men who have sex with men and transgender women attending trusted community centres in abuja and lagos, nigeria. | sexually transmitted infection (sti) and hiv prevalence have been reported to be higher amongst men who have sex with men (msm) in nigeria than in the general population. the objective of this study was to characterize the prevalence of hiv, chlamydia and gonorrhoea in this population using laboratory-based universal testing. | 2016 | 27931519 |
| menstrual cups and sanitary pads to reduce school attrition, and sexually transmitted and reproductive tract infections: a cluster randomised controlled feasibility study in rural western kenya. | conduct a feasibility study on the effect of menstrual hygiene on schoolgirls' school and health (reproductive/sexual) outcomes. | 2016 | 27881530 |
| leucocyte esterase dip-stick test as a point-of-care diagnostic for urogenital chlamydia in male patients: a multi-center evaluation in two sti outpatient clinics in paramaribo and amsterdam. | point-of-care (poc) tests are an important strategy to address the epidemic of sexually transmitted infections (stis). the leucocyte esterase test (let) can be used as a poc test for chlamydia. the aim of this study was to determine the diagnostic accuracy of the let to detect urogenital chlamydia among men at sti clinics in paramaribo, suriname and amsterdam, the netherlands. | 2016 | 27809795 |
| time to clearance of chlamydia trachomatis rna and dna after treatment in patients coinfected with neisseria gonorrhoeae - a prospective cohort study. | performing a test of cure (toc) could demonstrate success or failure of antimicrobial treatment of chlamydia trachomatis infection, but recommendations for the timing of a toc using nucleic acid amplification tests (naats) are inconsistent. we assessed time to clearance of c. trachomatis after treatment, using modern rna- and dna-based naats. | 2016 | 27724878 |
| lactobacillus crispatus inhibits the infectivity of chlamydia trachomatis elementary bodies, in vitro study. | lactobacillus species dominate the vaginal microbiota of healthy reproductive-age women and protect the genitourinary tract from the attack of several infectious agents. chlamydia trachomatis, a leading cause of sexually transmitted disease worldwide, can induce severe sequelae, i.e. pelvic inflammatory disease, infertility and ectopic pregnancy. in the present study we investigated the interference of lactobacillus crispatus, l. gasseri and l. vaginalis, known to be dominant species in the vagi ... | 2016 | 27354249 |
| polyethersulfone improves isothermal nucleic acid amplification compared to current paper-based diagnostics. | devices based on rapid, paper-based, isothermal nucleic acid amplification techniques have recently emerged with the potential to fill a growing need for highly sensitive point-of-care diagnostics throughout the world. as this field develops, such devices will require optimized materials that promote amplification and sample preparation. herein, we systematically investigated isothermal nucleic acid amplification in materials currently used in rapid diagnostics (cellulose paper, glass fiber, and ... | 2016 | 26906904 |
| a novel point-of-care testing strategy for sexually transmitted infections among pregnant women in high-burden settings: results of a feasibility study in papua new guinea. | sexually transmitted and genital infections in pregnancy are associated with an increased risk of adverse maternal and neonatal health outcomes. high prevalences of sexually transmitted infections have been identified among antenatal attenders in papua new guinea. papua new guinea has amongst the highest neonatal mortality rates worldwide, with preterm birth and low birth weight major contributors to neonatal mortality. the overall aim of our study was to determine if a novel point-of-care testi ... | 2016 | 27268218 |
| the performance of the vaginal discharge syndromic management in treating vaginal and cervical infection: a systematic review and meta-analysis. | this review aimed to synthesize and analyze the diagnostic accuracy and the likelihood of providing correct treatment of the syndromic approach vaginal discharge flowchart in managing cervical infections caused by neisseria gonorrhoeae (ng) and chlamydia trachomatis (ct), and vaginal infections caused by trichomonas vaginalis (tv) and bacterial vaginosis (bv) and candida albicans. this review will inform updating the who 2003 guidelines on vaginal discharge syndromic case management. | 2016 | 27706174 |
| validation of the genexpert® ct/ng assay for use with male pharyngeal and rectal swabs. | the genexpert(®) ct/ng (cepheid, sunnyvale, ca) assay is a point-of-care (poc) molecular diagnostic assay designed to rapidly test for the presence of chlamydia trachomatis (ct) and neisseria gonorrhoeae (gc). however, the test is only approved for vaginal swabs, urine, and endocervical swabs. here, we performed an evaluation of the genexpert(®) ct/ng assay to detect the presence of ct and gc on male pharyngeal and rectal swabs. | 2016 | 27536736 |
| changing pattern of chlamydia trachomatis strains in lymphogranuloma venereum outbreak, france, 2010-2015. | we describe a change in the molecular epidemiology of chlamydia trachomatis strains involved in an outbreak of rectal lymphogranuloma venereum in france during january 2010-april 2015. until 2012, the c. trachomatis l2b strain predominated; however, starting in 2013, most cases involved the l2 strain. we also identified 4 genetic l2b ompa variants. | 2016 | 27767927 |
| extragenital infections caused by chlamydia trachomatis and neisseria gonorrhoeae: a review of the literature. | in the united states, sexually transmitted diseases due to chlamydia trachomatis and neisseria gonorrhoeae continue to be a major public health burden. screening of extragenital sites including the oropharynx and rectum is an emerging practice based on recent studies highlighting the prevalence of infection at these sites. we reviewed studies reporting the prevalence of extragenital infections in women, men who have sex with men (msm), and men who have sex only with women (msw), including distri ... | 2016 | 27366021 |
| proportion of pelvic inflammatory disease cases caused by chlamydia trachomatis: consistent picture from different methods. | pelvic inflammatory disease (pid) is a leading cause of both tubal factor infertility and ectopic pregnancy. chlamydia trachomatis is an important risk factor for pid, but the proportion of pid cases caused by c. trachomatis is unclear. estimates of this are required to evaluate control measures. | 2016 | 27260786 |
| bacterial vaginosis: a synthesis of the literature on etiology, prevalence, risk factors, and relationship with chlamydia and gonorrhea infections. | bacterial vaginosis (bv) is a common vaginal disorder in women of reproductive age. since the initial work of leopoldo in 1953 and gardner and dukes in 1955, researchers have not been able to identify the causative etiologic agent of bv. there is increasing evidence, however, that bv occurs when lactobacillus spp., the predominant species in healthy vaginal flora, are replaced by anaerobic bacteria, such as gardenella vaginalis, mobiluncus curtisii, m. mulieris, other anaerobic bacteria and/or m ... | 2016 | 26877884 |
| the chlamydia trachomatis protease cpaf contains a cryptic pdz-like domain with similarity to human cell polarity and tight junction pdz-containing proteins. | the need for more effective anti-chlamydial therapeutics has sparked research efforts geared toward further understanding chlamydial pathogenesis mechanisms. recent studies have implicated the secreted chlamydial serine protease, chlamydial protease-like activity factor (cpaf) as potentially important for chlamydial pathogenesis. by mechanisms that remain to be elucidated, cpaf is directed to a discrete group of substrates, which are subsequently cleaved or degraded. while inspecting the previou ... | 2016 | 26829550 |
| tetracycline selective pressure and homologous recombination shape the evolution of chlamydia suis: a recently identified zoonotic pathogen. | species closely related to the human pathogen chlamydia trachomatis (ct) have recently been found to cause zoonotic infections, posing a public health threat especially in the case of tetracycline resistant chlamydia suis (cs) strains. these strains acquired a tet(c)-containing cassette via horizontal gene transfer (hgt). genomes of 11 cs strains from various tissues were sequenced to reconstruct evolutionary pathway(s) for tet(c) hgt. cs had the highest recombination rate of chlamydia species s ... | 2016 | 27576537 |
| community seroprevalence survey for yaws and trachoma in the western division of fiji. | both yaws and trachoma are endemic in several countries in the pacific. in co-endemic countries there may be potential synergies between both control programmes. | 2016 | 27852877 |
| risk factors associated with paraurethral duct dilatation following gonococcal paraurethral duct infection in men. | no studies have explored the risk factors for paraurethral duct dilatation following paraurethral duct infection by neisseria gonorrhoeae in men undergoing ceftriaxone therapy. the present study was performed to explore the risk factors for paraurethral duct dilatation following paraurethral duct infection by n. gonorrhoeae in men undergoing ceftriaxone therapy and thus guide clinical interventions. we compared the demographic, behavioral, and clinical data of men with paraurethral duct infectio ... | 2016 | 27861521 |
| comparison of clinical performance of antigen based-enzyme immunoassay (eia) and major outer membrane protein (momp)-pcr for detection of genital chlamydia trachomatis infection. | chlamydia trachomatis is the most common sexually transmitted bacterial pathogen worldwide. early detection and treatment of c.trachomatis genital infection prevent serious reproductive complications. | 2016 | 27525325 |
| association of human papillomavirus, neisseria gonorrhoeae and chlamydia trachomatis co-infections on the risk of high-grade squamous intraepithelial cervical lesion. | the link between high-risk human papillomavirus (hr-hpv) and other sexually transmitted diseases (stds) in the risk of developing cervical cancer still unclear. thus, in this report we investigated the rates of co-infections between hpv and other important non-hpv stds in different cervical findings using a multiplex polymerase chain reaction (m-pcr) to simultaneously detect chlamydia trachomatis, neisseria gonorrhoeae, mycoplasma genitalium, trichomonas vaginalis, hsv-1 and -2, and treponema pa ... | 2016 | 27429850 |
| prevalence of chlamydia trachomatis infection in parturient women in gipuzkoa, northern spain. | the prevalence of chlamydia trachomatis infection in southern europe is poorly understood and its identification is essential for the design of appropriate prevention policies. the prevalence of c. trachomatis in 2011-2014 was determined through polymerase chain reaction in urine samples from 11,687 unselected parturient women from the basque country, spain (san sebastián area). the overall age-adjusted prevalence was 1.0 % (95 % ci 0.8-1.2). the prevalence of infection in women younger than 25 ... | 2016 | 27247863 |
| chlamydia trachomatis infection in pregnancy: the global challenge of preventing adverse pregnancy and infant outcomes in sub-saharan africa and asia. | screening and treatment of sexually transmitted infections (stis) in pregnancy represents an overlooked opportunity to improve the health outcomes of women and infants worldwide. although chlamydia trachomatis is the most common treatable bacterial sti, few countries have routine pregnancy screening and treatment programs. we reviewed the current literature surrounding chlamydia trachomatis in pregnancy, particularly focusing on countries in sub-saharan africa and asia. we discuss possible chlam ... | 2016 | 27144177 |
| the association between cervical human papillomavirus infection and subsequent hiv acquisition in tanzanian and ugandan women: a nested case-control study. | this study was performed to analyze the associations between cervical human papillomavirus (hpv) infection and human immunodeficiency virus (hiv) acquisition, using cervical samples from previous studies in tanzania and uganda. | 2016 | 26951818 |
| association of human papillomavirus and chlamydia trachomatis with intraepithelial alterations in cervix samples. | the influence of different infectious agents and their association with human papillomavirus (hpv) in cervical carcinogenesis have not been completely elucidated. this study describes the association between cytological changes in cervical epithelium and the detection of the most relevant aetiological agents of sexually transmitted diseases. samples collected from 169 patients were evaluated by conventional cytology followed by molecular analysis to detect hpv dna, chlamydia trachomatis, herpes ... | 2016 | 26841046 |
| cytokine profile in nigerians with tubal infertility. | immune response to genital chlamydia trachomatis infection is involved in both immunity and pathology. the cytokine profile during infection has been implicated in the disease outcome, either resolution or severe sequelae. serum cytokines of chlamydia positive nigerian women with tubal infertility were assessed to determine their possible relationship with tubal occlusion. | 2016 | 27095929 |
| chlamydial pre-infection protects from subsequent herpes simplex virus-2 challenge in a murine vaginal super-infection model. | chlamydia trachomatis and herpes simplex virus-2 (hsv-2) genital tract co-infections have been reported in humans and studied in vitro but the clinical consequences are unknown. limited epidemiologic evidence suggests that these co-infections could be more severe than single infections of either pathogen, but the host-pathogen interactions during co-infection remain uncharacterized. to determine whether disease progression and/or pathogen shedding differs between singly-infected and super-infect ... | 2016 | 26726882 |
| coinfection of chlamydiae and other bacteria in reactive arthritis and spondyloarthritis: need for future research. | reactive (inflammatory) arthritis has been known for many years to follow genital infection with the intracellular bacterial pathogen chlamydia trachomatis in some individuals. recent studies from several groups have demonstrated that a related bacterium, the respiratory pathogen chlamydia pneumoniae, can elicit a similar arthritis. studies of these organisms, and of a set of gastrointestinal pathogens also associated with engendering inflammatory arthritis, have been relatively extensive. howev ... | 2016 | 27681924 |
| ctl0511 from chlamydia trachomatis is a type 2c protein phosphatase with broad substrate specificity. | protein phosphorylation has become increasingly recognized for its role in regulating bacterial physiology and virulence. chlamydia spp. encode two validated hanks'-type ser/thr protein kinases, which typically function with cognate protein phosphatases and appear capable of global protein phosphorylation. consequently, we sought to identify a ser/thr protein phosphatase partner for the chlamydial kinases. ctl0511 from chlamydia trachomatis l2 434/bu, which has homologs in all sequenced chlamydi ... | 2016 | 27114464 |
| human gbp1 does not localize to pathogen vacuoles but restricts toxoplasma gondii. | guanylate binding proteins (gbps) are a family of large interferon-inducible gtpases that are transcriptionally upregulated upon infection with intracellular pathogens. murine gbps (mgbps) including mgbp1 and 2 localize to and disrupt pathogen-containing vacuoles (pvs) resulting in the cell-autonomous clearing or innate immune detection of pv-resident pathogens. human gbps (hgbps) are known to exert antiviral host defense and activate the nlrp3 inflammasome, but it is unclear whether hgbps can d ... | 2016 | 26874079 |
| multiplex strand invasion based amplification (msiba) assay for detection of chlamydia trachomatis and neisseria gonorrhoeae. | nucleic acid amplification tests have become a common method for diagnosis of stis due to their improved sensitivity over immunoassays and traditional culture-based methods. isothermal nucleic acid amplification methods offer significant advantages over polymerase chain reaction (pcr) because they do not require sophisticated instruments needed for thermal cycling of pcr. we recently reported a novel isothermal nucleic acid amplification method, strand invasion-based amplification (siba), which ... | 2016 | 26837460 |
| bioprospecting sponge-associated microbes for antimicrobial compounds. | sponges are the most prolific marine organisms with respect to their arsenal of bioactive compounds including antimicrobials. however, the majority of these substances are probably not produced by the sponge itself, but rather by bacteria or fungi that are associated with their host. this review for the first time provides a comprehensive overview of antimicrobial compounds that are known to be produced by sponge-associated microbes. we discuss the current state-of-the-art by grouping the bioact ... | 2016 | 27144573 |
| intra-chip: studying gene regulation in an intracellular pathogen. | intracellular bacteria that reside within a host cell use a variety of strategies to exploit this unique niche. while these organisms are technically challenging to study in the context of an infected host cell, recent advances have led to an improved understanding of how the intracellular environment impacts bacterial gene expression. we recently demonstrated that chromatin immunoprecipitation (chip) can be used to quantify transcription factor binding in the obligate intracellular pathogen chl ... | 2016 | 26886234 |
| extraction of cobalt, iron, indium and zinc from seawater by means of the trifluoroacetylacetone-toluene system. | 2016 | 5082955 | |
| what is overpopulation? | 2016 | 5069217 | |
| the validity study of the leighton instrument. | 2016 | 5063405 | |
| marihuana--a smoke screen. | 2016 | 5063403 | |
| radical surgery for carcinoma of the middle ear. | 2016 | 5053991 | |
| plasma cortisol, total bound and unbound in severe malnutrition in children. | 2016 | 5050342 | |
| [value of electrophysiological investigation for therapeutic indications in leprous neuritis]. | 2016 | 5047450 | |
| synthesis and proton magnetic resonance spectrum of a selectively deuterated dinucleoside monophosphate, adenylyl-(3'-5')-adenosine. | 2016 | 5038401 | |
| liver scanning as a method for detecting primary liver cancer. report on 100 cases. | 2016 | 5054262 | |
| history of infection and immunity to rubella. | 2016 | 5039729 | |
| pyruvate blood levels after glucose overload in prediabetes and diabetes. | 2016 | 5038946 | |
| biological and ecological influences on development at 24 and 36 months of age. | 2016 | 5055912 | |
| studies on the ureide metabolism of germinating oilseeds and legumes. | 2016 | 5085636 | |
| a dietitian with the s.s. "hope". | 2016 | 5033384 | |
| [observations on serum-vaccination in the prevention of tetanus]. | 2016 | 5031451 | |
| fatal cases of 'acute abdomen'. | 2016 | 5093790 | |
| the anaesthetic management of tracheo-oesophageal fistula: a review of ten years' experience. | 2016 | 5029041 | |
| [changes in renal function following repeat transfusions of stored blood after diverse periods of preservation]. | 2016 | 5028020 | |
| outcome of illegitimate conceptions in a black population. | field interviews were conducted to gather data on pregnancy wastage and illegitimacy among 990 urban black women in a north carolina standard metropolitan statistical area. the sample range in age from 15-39, was at least gravida 1, and was at the poverty level (based on relationship of family size to income). 90% of births were illegitimate with little influence from economic status or income, but higher educational levels were associated with a greater percentage of conceptions resulting in il ... | 2016 | 5098489 |
| content analysis of speech samples to determine effect of lorazepam on anxiety. | 2016 | 5026373 | |
| [rheumatoid nephropathy]. | 2016 | 5018167 | |
| unidirectional intraaortic balloon pumping in cardiogenic shock and intractable left ventricular failure. | 2016 | 5058349 | |
| myocardial infarction in oslo 1967-69. incidence and case fatality. | 2016 | 5031858 |