Publications

serological testing for bartonella henselae infections in the netherlands: clinical evaluation of immunofluorescence assay and elisa.	cat-scratch disease (csd), caused by bartonella henselae infection, can mimic malignancy and can manifest atypically. reliable serological testing is therefore of great clinical importance. the diagnostic performance of immunofluorescence assay (ifa) and elisa was evaluated in a group of dutch patients with proven csd (clinical diagnosis confirmed by pcr). sera of 51 csd patients and 56 controls (patients with similar symptoms, but who were b. henselae pcr-negative and had an alternative confirm ...	2007	17378931
development of discriminatory multiple-locus variable number tandem repeat analysis for bartonella henselae.	bartonella henselae is a zoonotic bacterium that infects cats and humans. several attempts have been made to develop typing techniques for epidemiological purposes; however, most of the techniques developed do not appear to be sufficiently discriminatory or easy to use. in order to develop multilocus variable number tandem repeat (vntr) analysis (mlva) for b. henselae, 30 vntr candidates were selected from the genome sequence of the reference strain houston 1 (h1). the vntr candidates were initi ...	2007	17379723
evaluation of pulsed-field gel electrophoresis and multi-locus sequence typing for the analysis of clonal relatedness among bartonella henselae isolates.	pulsed-field gel electrophoresis (pfge) represents the gold standard among band-based methods for the molecular typing of bartonella henselae. smai and noti have been frequently used for typing b. henselae by pfge. however, their appropriateness for the analysis of genetic relatedness among b. henselae isolates has not been assessed systematically hitherto. aim of the present study was to evaluate smai, noti, and three additional endonucleases for typing b. henselae isolates by pfge and to compa ...	2007	17398157
chronic vasculitis and polyneuropathy due to infection with bartonella henselae.	bartonella henselae, the causative agent of cat scratch disease and bacillary angiomatosis, is associated with an expanding spectrum of diseases. here, we report on a 40-year-old patient suffering from chronic recurrent painful ulcers of the toes, distal axonal sensomotor polyneuropathy and raynaud's phenomenon. biopsy of the sural nerve demonstrated an axonal neuropathy with a neurogenic muscular atrophy. treatment with high dose corticosteroids had no beneficial effect. a biopsy taken from a r ...	2007	17401716
controlled trial of a 5-day course of telithromycin versus doxycycline for treatment of mild to moderate scrub typhus.	new antibiotics are required to have the antibacterial activity against doxycycline-resistant orientia tsutsugamushi. an in vitro sensitivity study showed that telithromycin was more effective than erythromycin for rickettsia, bartonella, and coxiella burnetii. in this prospective, open-label, randomized trial, we enrolled patients with mild-to-moderate scrub typhus. we compared the efficacy and safety of a 5-day telithromycin therapy with those of a 5-day doxycycline therapy at chosun universit ...	2007	17404000
presence of bartonella spp. in various human populations.	bartonella spp. bacteria are significant human pathogens and the agents of bacterial zoonosis acquired from an animal companion. the aim of the study was to determine the seroprevalence of two of the most common bartonella species b. henselae and b. quintana in various human populations. the studied groups included: alcoholics, intravenous drug users, veterinarians, cats' owners. blood samples were collected and cultured on chocolate agar plates and in mouse fibroblasts l-929 cell line culture. ...	2007	17419187
detection of rickettsia rickettsii and bartonella henselae in rhipicephalus sanguineus ticks from california.	sixty-two questing adult rhipicephalus sanguineus (latreille) ticks were collected by direct removal from blades of turfgrass and adjacent concrete walkways at a suburban home in riverside county, ca, and tested for the presence of rickettsia, bartonella, and ehrlichia dna. polymerase chain reaction (pcr) was used to amplify fragments of the 17-kda antigen gene and the rompa gene of the spotted fever group rickettsiae. one male tick contained r. rickettsii dna; its genotype differed from r. rick ...	2007	17294935
an hiv-positive patient with cervical lymphadenopathy and skin lesions.		2007	17304465
proteomic and immunoblot analyses of bartonella quintana total membrane proteins identify antigens recognized by sera from infected patients.	bartonella quintana is a fastidious, gram-negative, rod-shaped bacterium that causes prolonged bacteremia in immunocompetent humans and severe infections in immunocompromised individuals. we sought to define the outer membrane subproteome of b. quintana in order to obtain insight into the biology and pathogenesis of this emerging pathogen and to identify the predominant b. quintana antigens targeted by the human immune system during infection. we isolated the total membrane proteins of b. quinta ...	2007	17307937
living without fur: the subtlety and complexity of iron-responsive gene regulation in the symbiotic bacterium rhizobium and other alpha-proteobacteria.	the alpha-proteobacteria include several important genera, including the symbiotic n(2)-fixing "rhizobia", the plant pathogen agrobacterium, the mammalian pathogens brucella, bartonella as well as many others that are of environmental or other interest--including rhodobacter, caulobacter and the hugely abundant marine genus pelagibacter. only a few species--mainly different members of the rhizobia--have been analyzed directly for their ability to use and to respond to iron. these studies, howeve ...	2007	17310401
adult systemic cat scratch disease associated with therapy for hepatitis c.	we describe the first case of systemic cat scratch disease in a patient receiving peginterferon alpha-2a and ribavirin for treatment of hepatitis c. cases of adult systemic csd are extremely infrequent and immunomodulatory treatment for hepatitis c has been associated with aberrant host responses to common pathogens.	2007	17319959
experimental infection of domestic cats with passaged genotype i bartonella henselae.	the influence of in vitro passage on bartonella henselae pathogenesis in cats has not been thoroughly evaluated. our objective was to examine the bacterial kinetics and humoral immune responses in cats experimentally infected with three different in vitro passages of b. henselae f1, a genotype i strain of feline origin. the f1 strain was in vitro passaged 20 and 40 times, and each was inoculated into a group of 5 cats. the kinetics of bacteremia and the feline humoral immune response to bacteria ...	2007	17321078
detection of bartonella henselae dna in two dogs with pyogranulomatous lymphadenitis.	1 dog evaluated because of inappetence and lameness of the left hind limb of 1 day's duration and 1 dog evaluated because of inappetence, fever, and lymphadenopathy of 2 weeks' duration.	2007	17331050
recurrent expressive aphasia as a presentation of cat-scratch encephalopathy.	cat-scratch disease is a common disease, occurring in an estimated 24,000 patients annually in the united states, and is one of the most common causes of chronic lymphadenitis in children. a wide array of neurologic complications occurs as a result of cat-scratch disease. however, there have been no reports of acute-onset, self-resolving, recurrent, expressive aphasia, as we report here in an adolescent boy. in our case, establishing the diagnosis of cat-scratch encephalopathy saved time and res ...	2007	17332191
cat scratch disease during etanercept therapy.	lymphadenopathy in a patient taking tnfalpha antagonist therapy may indicate lymph node tuberculosis or a hematological malignancy. although histological evidence of granuloma suggests tuberculosis, many other infections responsible for granulomatous reactions have been reported in patients on tnfalpha antagonist therapy. we report a case of granulomatous lymphadenopathy due to bartonella henselae in a patient taking etanercept to treat ankylosing spondylitis.	2007	17337229
cat scratch disease with epidural extension while on antimicrobial treatment.	we report the first case of a child with an epidural abscess caused by bartonella henselae. this case is unique in that the diagnosis was confirmed by polymerase chain reaction (pcr) of the abscess fluid. a 3-year-old male was admitted with a 1-week history of fever and lower back pain. magnetic resonance imaging (mri) of the lumbar spine revealed a paraspinal soft tissue abnormality extending from the t(12) to the l(4) level, and a computed tomography (ct) scan of the lumbar spine showed osteom ...	2007	17337934
bone infection in cat-scratch disease: a review of the literature.	to describe the main features of bone infection associated with cat-scratch disease (csd).	2007	17140668
pyogenic splenic abscess in an infant with serological evidence of cat scratch disease.		2007	17186270
an immunohistochemical and polymerase chain reaction evaluation of feline plasmacytic pododermatitis.	sections of 14 skin biopsies of cats with plasmacytic pododermatitis and a clinical follow-up of 12-36 months were stained with a polyclonal anti-mycobacterium bovis (bacille calmette-guerin = bcg) antibody cross-reactive to a broad spectrum of fungi and bacteria. all sections were negative for organisms within the actual footpad tissue with the anti-bcg antibody stains. polymerase chain reaction (pcr) assays that amplify the dna of bartonella spp., ehrlichia spp., anaplasma phagocytophilum, chl ...	2007	17197627
a survey of zoonotic pathogens carried by norway rats in baltimore, maryland, usa.	norway rats (rattus norvegicus) carry several zoonotic pathogens and because rats and humans live in close proximity in urban environments, there exists potential for transmission. to identify zoonotic agents carried by rats in baltimore, maryland, usa, we live-trapped 201 rats during 2005-2006 and screened them for a panel of viruses, bacteria, and parasites. antibodies against seoul virus (57.7%), hepatitis e virus (hev, 73.5%), leptospira interrogans (65.3%), bartonella elizabethae (34.1%), a ...	2007	17224086
rickettsial pathogens in the tropical rat mite ornithonyssus bacoti (acari: macronyssidae) from egyptian rats (rattus spp.).	we collected and tested 616 tropical rat mites (ornithonyssus bacoti (hirst)) from rats (rattus norvegicus (berkenhout) and r. rattus (linnaeus)) throughout 14 governorates in egypt and tested dna extracts from pools of these mites for bartonella spp., coxiella burnetii, and rickettsia spp. by pcr amplification and sequencing. three different mite-associated bacterial agents, including one bartonella and two rickettsia spp., were detected in eight pools of mites. further research could demonstra ...	2007	17225079
cat-scratch disease relapse in a kidney transplant recipient.	cat-scratch disease, an infectious illness infrequently reported in kidney transplant patients, is caused by the organism bartonella henselae and is transmitted through contact with cats or kittens. it is a self-limited disorder in the general pediatric population. here we present a case of unsuspected cat-scratch disease in a pediatric kidney transplant patient who presented with fever and lymphadenopathy. eight months after treatment with a short course of azithromycin, the patient developed a ...	2007	17239132
cat scratch disease causing hepatic masses after liver transplant.	hepatic cat scratch disease is rarely reported in liver transplant recipients and has never been reported with discrete liver lesions in the graft. a 52-year-old woman was transplanted for hepatitis c cirrhosis and hepatocellular carcinoma. her posttransplant course was uneventful. she presented 2.7 years after transplantation with fever of unknown origin and went on to develop multiple and diffuse discrete liver lesions. despite an extensive work-up including percutaneous and laparoscopic biops ...	2007	17241393
bartonella henselae infection in splenectomized domestic cats previously infected with hemotropic mycoplasma species.	cat scratch disease is caused by bartonella henselae and the domestic cat represents its main reservoir. in immunocompromised patients, infection with b. henselae is characterized by more severe clinical forms than in non-immunocompromised individuals. the objective of the present study was to investigate the characteristics of b. henselae (houston-i strain) infection in four splenectomized and three non-splenectomized cats, five of which were chronically infected with 'candidatus mycoplasma hae ...	2007	17254639
high suspicion of bacillary angiomatosis in a kidney transplant recipient: a difficult way to diagnose--case report.	bacillary angiomatosis is an infection caused by bartonella, which has first been described in human immunodeficiency virus (hiv)-infected patients. we report an unusually located lesion, in a totally asymptomatic kidney transplant recipient. the diagnosis was strongly suggested based on the iconography and our histological analysis, but was not confirmed using polymerase chain reaction (pcr) and immunohistochemical studies. we illustrate our difficult way to the diagnosis as well as the course ...	2007	17275532
haemoparasites of common shrews (sorex araneus) in northwest england.	the presence of haemoparasites belonging to the taxa anaplasma, bartonella and trypanosoma was determined among 76 common shrews (sorex araneus) from northwest england. anaplasma phagocytophilum dna was recovered from the blood of 1 shrew (1.3%), with the amplified 16s rrna sequence identical to one previously reported from a bank vole (clethrionomys glareolus). trypanosoma spp. dna was detected in 9 shrews (11.8%), the amplified 18s rdna fragments being indistinguishable from one another, and d ...	2007	17288633
contrasting dynamics of bartonella spp. in cyclic field vole populations: the impact of vector and host dynamics.	many zoonotic disease agents are transmitted between hosts by arthropod vectors, including fleas, but few empirical studies of host-vector-microparasite dynamics have investigated the relative importance of hosts and vectors. this study investigates the dynamics of 4 closely related bartonella species and their flea vectors in cyclic populations of field voles (microtus agrestis) over 3 years. the probability of flea infestation was positively related to field vole density 12 months previously i ...	2007	17096870
characterization of th1 activation by bartonella henselae stimulation in balb/c mice: inhibitory activities of interleukin-10 for the production of interferon-gamma in spleen cells.	this study was conducted to analyze cytokine production mechanisms in mice after bartonella henselae stimulation. balb/c mice were inoculated intraperitoneally with 3 x 10(6) colony forming units of b. henselae (houston-1 strain) twice at 10-day interval. spleen cells were harvested from the mice and stimulated with the organisms. following the stimulation, interferon-gamma (ifn-gamma) and interleukin-4 (il-4), il-10, il-12 and tumor necrosis factor-alpha (tnf-alpha) were measured in the culture ...	2007	17005337
the identification of two protective dna vaccines from a panel of five plasmid constructs encoding brucella melitensis 16m genes.	five candidate genes from the brucella melitensis 16m genome were selected. eukaryotic expression plasmids encoding these antigens were constructed and expression was verified in vitro from transfected cos7 cells. each vaccine was assessed for protective efficacy in a balb/c mouse brucellosis infection model. from these experiments two protective dna vaccines were identified: p-omp25 and p-ialb. the omp25 antigen (bmei1249) has previously been studied in terms of brucella virulence, serodiagnosi ...	2007	17049676
analysis of bartonella adhesin a expression reveals differences between various b. henselae strains.	bartonella henselae causes cat scratch disease and the vasculoproliferative disorders bacillary angiomatosis and peliosis hepatis in humans. one of the best known pathogenicity factors of b. henselae is bartonella adhesin a (bada), which is modularly constructed, consisting of head, neck/stalk, and membrane anchor domains. bada is important for the adhesion of b. henselae to extracellular-matrix proteins and endothelial cells (ecs). in this study, we analyzed different b. henselae strains for ba ...	2007	17060468
isolation of bartonella quintana from a woman and a cat following putative bite transmission.	we report here the detection of bartonella quintana, after putative bite transmission, in pre-enrichment blood cultures from a woman and from two feral barn cats. prospective molecular epidemiological studies are necessary to characterize the risk of human bartonella quintana infection following cat bites.	2007	17093037
bone marrow and skin granulomatosis in a patient with bartonella infection.	this report describes a case of granulomatous inflammation, involving the bone marrow and skin, due to bartonella infection in an immunocompetent patient. the clinical presentation included prolonged fever, pancytopenia, rash and hepatitis. bartonella infection should thus be added to the growing list of entities that produce marrow granulomas and fever.	2007	17172528
effect of high-dose methyl-prednisolone on brainstem encephalopathy and basal ganglia impairment complicating cat scratch disease.	cat scratch disease (csd) is a zoonotic illness caused by the gram negative bacillus bartonella henselae characterized by a small skin lesion at the site of a bite, lick or scratch by a cat, commonly followed by regional lymphadenopathy 1 or 2 weeks later. we report herein on severe neurological complications of csd combining brainstem encephalopathy and basal ganglia impairment. this 12-year-old female acutely presented to a local hospital with profound coma and a prolonged tonic posturing of e ...	2007	17174500
an unusual cutaneous presentation of cat-scratch disease.		2007	17176268
prevalence of mycoplasma haemofelis, 'candidatus mycoplasma haemominutum', bartonella species, ehrlichia species, and anaplasma phagocytophilum dna in the blood of cats with anemia.	hemoplasmas are known causes of anemia in some cats and some bartonella species have been associated with anemia in people and in dogs. in this retrospective study, we used polymerase chain reaction (pcr) assays to determine the prevalence rates of mycoplasma haemofelis, 'candidatus m haemominutum', a phagocytophilum, ehrlichia species, and bartonella species dna in the blood of cats with anemia and a control group of healthy cats. dna of the organisms was amplified from 22 of 89 cats with anemi ...	2007	16846745
bartonella native valve endocarditis: the first brazilian case alive and well.	bartonella is an important cause of blood culture-negative endocarditis in recent studies. seroprevalence studies in the states of minas gerais and rio de janeiro have shown bartonella igg positivity around 14% in healthy adults and 40% in hiv seropositive adults, respectively. a case report of a 46-year-old white male with moderate aortic regurgitation (ar) due to rheumatic heart disease (rhd), admitted due to worsening heart failure, is presented. clinical features were apyrexia, anemia, polyc ...	2007	18327472
co-circulating microorganisms in questing ixodes scapularis nymphs in maryland.	ixodes scapularis can be infected with borrelia burgdorferi, anaplasma phagocytophilum, bartonella spp., babesia microti, and rickettsia spp., including spotted-fever group rickettsia. as all of these microorganisms have been reported in maryland, the potential for these ticks to have concurrent infections exists in this region. to assess the frequency of these complex infections, 348 i. scapularis nymphs collected in 2003 were screened for these microorganisms by pcr with positives being confir ...	2007	18260514
musculoskeletal manifestations of cat scratch disease.	musculoskeletal manifestations (mms) are considered to be rare in cat scratch disease (csd) and are not well characterized. we aimed to study mms of csd.	2007	18190312
from cat scratch disease to bartonella henselae infection.		2007	18190313
molecular evidence of bartonella dna in ixodid ticks in czechia.	we report the development of a novel method for detection of bartonella dna in ixodid ticks. the assay is based on a specific amplification of a part of 16s rrna gene and 16s-23s rrna intergenic spacer region of bartonella sp. by nested pcr and southern blot hybridization with specific dna probe; the method is highly sensitive and specific. the screening of 327 unfed ticks collected in different urban and suburban areas of czechia in 2003-2005 revealed the presence of bartonella dna in four ixod ...	2007	18298048
[persistent bartonella infection: epidemiological and clinical implications].	bartonella are gram-negative hemotropic bacteria that infect a wide range of mammals. at least 14 bartonella species or subspecies have been reported to be pathogenic for humans and animals. wild and domestic animals represent a large reservoir. reservoir species usually display chronic bacteremia. this explains some aspects of the epidemiology of these infections, and especially vector-borne transmission. the molecular mechanisms of persistent infection have clinical consequences both for occas ...	2007	18402163
bartonella endocarditis in complex congenital heart disease.	bartonella species are an important cause of culture-negative endocarditis, with recognized risk factors of alcoholism, homelessness, cat exposure, and pre-existing valvular disease. we report a case of bartonella henselae endocarditis in a 36-year-old woman with complex congenital heart disease who presented with a 7-month history of hemolytic anemia, leukocytoclastic vasculitis, and recurrent fevers. transesophageal echocardiogram revealed vegetations on the patient's native aortic valve and i ...	2007	18377522
american association of feline practitioners 2006 panel report on diagnosis, treatment, and prevention of bartonella spp. infections.		2006	16846781
bartonella spp. in deer keds, lipoptena mazamae (diptera: hippoboscidae), from georgia and south carolina, usa.	deer keds, lipoptena mazamae (diptera: hippoboscidae), were collected from white-tailed deer (odocoileus virginianus) and humans in georgia and south carolina, usa (1 october 2001-6 january 2005) and screened for the presence of dna from bartonella spp. forty deer keds were screened for bartonella spp. by polymerase chain reaction using primers specific to the riboflavin synthase gene (ribc) of bartonella. bartonella species closely related to bartonella schoenbuchensis and to the etiologic agen ...	2006	16870863
first evidence of feline herpesvirus, calicivirus, parvovirus, and ehrlichia exposure in brazilian free-ranging felids.	serum samples from 18 pumas (puma concolor), one ocelot (leopardus pardalis), and two little spotted cats (leopardus tigrinus) collected from free-ranging animals in brazil between 1998 and 2004 were tested by indirect immunofluorescence (ifa) for antibodies to feline herpesvirus 1 (fhv 1), calicivirus (fcv), coronavirus (fcov), parvo-virus (fpv), ehrlichia canis, anaplasma pha-gocytophilum, and bartonella henselae. serum samples also were tested, by western blot and elisa, for feline leukemia v ...	2006	16870878
[study on bartonella vinsonii berkhoffii isolated from blood of native dogs in china].	to isolate and identify bartonella strains from native dogs in shandong province in china.	2006	16875539
cloning, characterization, and expression of bartonella henselae p26.	in order to identify immunoreactive bartonella henselae proteins, b. henselae antiserum from an experimentally infected cat was used to screen a b. henselae genomic dna expression library. one immunoreactive phage clone contained a gene (p26) with significant nucleotide identity with orthologs in brucellae, bartonellae, and several plant-associated bacteria. p26 gene sequences from four b. henselae strains, one b. koehlerae strain, and one b. clarridgeiae strain were cloned. comparative nucleoti ...	2006	16893981
broad-range polymerase chain reaction for the diagnosis of bartonella henselae endocarditis.	we present a case of subacute bacterial endocarditis in a 10-year-old girl with di-george syndrome, congenital heart disease, and mild immunodeficiency. she was afebrile at initial presentation but was found to have massive splenomegaly, and signs of congestive heart failure. no causative organism could be identified on routine blood and tissue cultures. a detailed clinical history revealed a history that she had been scratched by a cat and developed intermittant fevers over 3 months. bartonella ...	2006	16898887
description of pseudochrobactrum gen. nov., with the two species pseudochrobactrum asaccharolyticum sp. nov. and pseudochrobactrum saccharolyticum sp. nov.	two gram-negative, rod-shaped, oxidase-positive, non-spore-forming, non-motile bacteria (ccug 46016(t) and ccug 33852(t)), isolated from a knee aspirate of a 66-year-old man and an industrial glue, respectively, were studied for their taxonomic position. on the basis of chemotaxonomic data [i.e. major ubiquinone (q-10), major polar lipids (phosphatidylethanolamine, phosphatidylglycerol and phosphatidylcholine) and major fatty acids (c(18 : 1)omega7c and c(19 : 0) cyclo omega8c)] and 16s rrna gen ...	2006	16902015
ixodes frontalis and avian tick-related syndrome in the united kingdom.	this study aimed to characterise tick species responsible for avian tick infestations in the uk, to analyse various risk factors for tick-related syndrome in tick-infested birds and to test samples for the presence of certain tick-transmitted pathogens.	2006	16911113
in vitro susceptibility of bartonella species to 17 antimicrobial compounds: comparison of etest and agar dilution.	in vitro susceptibility testing of 31 bartonella spp. strains including 21 bartonella henselae isolates was performed for 17 antimicrobial agents (telithromycin, four macrolides, five fluoroquinolones, five aminoglycosides, doxycycline and rifampicin).	2006	16916864
serological and molecular evidence of exposure to arthropod-borne organisms in cats from northeastern spain.	one hundred sixty-eight cat sera from spain were tested for igg antibodies to rickettsia conorii (rc), ehrlichia canis (ec), anaplasma phagocytophilum (ap) and bartonella henselae (bh) antigens using ifa and for felv antigen and fiv antibody by elisa. for 47 whole blood samples, pcr testing was performed for rickettsia, ehrlichia and bartonella. seroprevalences were: bh (71.4%), rc (44%), ec (11.3%), felv (8.5%), fiv (7.4%) and ap (1.8%). bh antibodies were associated with seroreactivity to both ...	2006	16919405
[a case of cat scratch disease in a patient with diabetes mellitus and chronic renal failure].	the article describes a case of cat scratch disease in a female patient with severe chronic pathology. this relatively rare disease was manifested by regional (in the site of microbial intrusion) lymphadenopathy, and general infectious syndrome. the presence of oncohematological process was excluded without biopsy; serologic examination with bartonella antibodies confirmed the diagnosis. combined treatment with two antibiotics, rifampicin and doxycycline, was successful. the article stresses tha ...	2006	16924805
bartonella quintana variably expressed outer membrane proteins mediate vascular endothelial growth factor secretion but not host cell adherence.	bartonella quintana causes trench fever, endocarditis, and the vasculoproliferative disorders bacillary angiomatosis and peliosis hepatis in humans. little is known about the interaction of this pathogen with host cells. we attempted to elucidate the interaction of b. quintana with human macrophages (thp-1) and epithelial cells (hela 229). remarkably, only b. quintana strain jk-31 induced secretion of vascular endothelial growth factor (vegf) from thp-1 and hela 229 cells upon infection similar ...	2006	16926391
autocrine role for interleukin-8 in bartonella henselae-induced angiogenesis.	the gram-negative bacterium bartonella henselae is capable of causing angiogenic lesions as a result of infection. previously, it has been shown that b. henselae infection can result in production of the chemokine interleukin-8 (il-8). in this study, we demonstrated that monocytes, endothelial cells, and hepatocytes produce il-8 in response to b. henselae infection. we also investigated the role of il-8 in b. henselae-induced endothelial cell proliferation and capillary tube formation. both in v ...	2006	16926411
systemic cat scratch disease.	systemic cat scratch disease (csd) is often associated with prolonged fever and microabscesses in the liver and/or spleen. we report a case of systemic csd with hepatic, splenic and renal involvement in an aboriginal child in taiwan. a previously healthy 9-year-old girl had an intermittent fever for about 17 days, and complained of abdominal pain, headache and weight loss. abdominal computed tomography showed multiple tiny hypodense nodular lesions in the spleen and both kidneys. laparotomy reve ...	2006	16935770
genome rearrangements, deletions, and amplifications in the natural population of bartonella henselae.	cats are the natural host for bartonella henselae, an opportunistic human pathogen and the agent of cat scratch disease. here, we have analyzed the natural variation in gene content and genome structure of 38 bartonella henselae strains isolated from cats and humans by comparative genome hybridizations to microarrays and probe hybridizations to pulsed-field gel electrophoresis (pfge) blots. the variation in gene content was modest and confined to the prophage and the genomic islands, whereas the ...	2006	16936024
molecular characterization of resistance to macrolides in bartonella henselae.	we selected in vitro erythromycin-resistant strains of bartonella henselae. the mutants obtained had point mutations in domain v of 23s rrna and/or in ribosomal protein l4. one lymph node of a patient with cat-scratch disease had such a mutation in 23s rrna, suggesting that natural resistant strains may infect humans.	2006	16940128
case records of the massachusetts general hospital. case 27-2006. a 17-year-old boy with fever and lesions in the liver and spleen.		2006	16943407
analysis of data in 30 patients with cat scratch disease without lymphadenopathy.	the prominent clinical manifestation of cat scratch disease is regional lymphadenopathy at the site of the cat scratch or bite, associated with fever or general symptoms. a serological study of 540 patients with either lymphadenopathy, persistent fever, or pet ownership disclosed that 30 (16.1%) of the 186 patients with a serological diagnosis of cat scratch disease had no regional lymphadenopathy, and in these 30 patients, the absence of lymphadenopathy was closely related to the presence of pe ...	2006	16944264
prevalence of dna of mycoplasma haemofelis, 'candidatus mycoplasma haemominutum,' anaplasma phagocytophilum, and species of bartonella, neorickettsia, and ehrlichia in cats used as blood donors in the united states.	to identify the prevalence of dna of mycoplasma haemofelis; 'candidatus mycoplasma haemominutum'; anaplasma phagocytophilum; and species of bartonella, neorickettsia, and ehrlichia in blood of cats used as blood donors in the united states.	2006	16948578
prevalence of bartonella henselae antibodies in serum of cats with and without clinical signs of central nervous system disease.	bartonella henselae is occasionally associated with neurological dysfunction in people and some experimentally infected cats. the purpose of this study was to determine whether b henselae seroprevalence or titer magnitude varies among cats with neurological disease, cats with non-neurological diseases, and healthy cats while controlling for age and flea exposure. there was no difference in b henselae seroprevalence rates between cats with seizures and cats with other neurological diseases. cats ...	2006	16949848
[a case of hepatosplenic cat scratch disease].	a 43-year-old man was admitted with idiopathic fever. abdominal ultrasonogram demonstrated multiple hypoechoic lesions in the spleen. abdominal ct scan showed multiple hypodense lesions in the liver and spleen. the patient had a cat in his house, and the presence of a very high serous antibody titer for bartonella henselae led to the diagnosis of hepatosplenic cat scratch disease. it is important to consider this disease in the differential diagnosis of idiopathic fever when multiple lesions are ...	2006	16953102
alpha1-proteinase inhibitor deficiency and bartonella infection in association with panniculitis, polyarthritis, and meningitis in a dog.		2006	16955834
[hepatosplenic localization of cat scratch disease: two cases in immunocompetent adult patients].	cat-scratch disease (csd) is a well-recognized benign cause of localized lymphadenopathy, which often recovers spontaneously. however systemic clinical presentations are described in immunodeficient adults (bacillary angiomatosis, bacillary splenitis) and are less common in immunocompetent ones.	2006	16959382
bartonella quintana prosthetic valve endocarditis detected by blood culture incubation beyond 10 days.	we describe the case of a 45-year-old man with mitral and aortic prosthetic valve replacement who presented with symptoms of subacute bacterial endocarditis. bartonella quintana was grown from blood after prolonged culture. the course of the disease was complicated by splenic infarction, glomerulonephritis resulting in progressive renal insufficiency, and cerebroventricular hemorrhage. notably, cardiac ultrasonography showed no extensive vegetations but a strand-like lesion. culture-positive b. ...	2006	16962956
somatostatin receptor scintigraphy in patients with cat-scratch disease.	somatostatin receptor scintigraphy images various neoplastic, granulomatous, and auto-immune diseases. cat-scratch disease in an infectious granulomatous disease usually affecting the lymphnodes. it is not known whether cat-scratch disease provides positive somatostatin receptor scintigrams.	2006	16964341
[cat scratch disease--course, diagnosis].	cat scratch disease is an infection which often causes regional lymphadenopathy. bartenolloses present a growing health problem both in human and veterinary medicine. they may be etiological fevers of unknown etiology, endocarditis, pneumonia, meningitis. the mildest clinical form is the cat scratch disease which proceeds as a mild regional lymphadenopathy. it is caused by bartonellosis henselae. animals, especially cats, are the sourse of infections. lymphadenitis may remain for many weeks and ...	2006	16964683
rickettsial agents in egyptian ticks collected from domestic animals.	to assess the presence of rickettsial pathogens in ticks from egypt, we collected ticks from domestic and peridomestic animals between june 2002 and july 2003. dna extracts from 1019 ticks were tested, using pcr and sequencing, for anaplasma spp., bartonella spp., coxiella burnetii, ehrlichia spp., and rickettsia spp. ticks included: 29 argas persicus, 10 hyalomma anatolicum anatolicum, 55 hyalomma anatolicum excavatum, 174 hyalomma dromedarii, 2 hyalomma impeltatum, 3 hyalomma marginatum rufipe ...	2006	17004028
emergence of distinct genetic variants in the population of primary bartonella henselae isolates.	bartonella henselae isolates from different hosts display a marked genetic heterogeneity, as determined by pulsed-field gel electrophoresis (pfge). the aim of the present study was to determine whether different genetic variants may coexist within the population of distinct b. henselae isolates and could be detected by pfge. three primary b. henselae isolates and the b. henselae reference strains atcc 49793 and 49882 were subjected as single colony derived cultures in quadruplicate to pfge analy ...	2006	16697237
autoimmunohistochemistry: a new method for the histologic diagnosis of infective endocarditis.	although the pathologic examination of cardiac valves remains the reference standard for the diagnosis of infective endocarditis, the detection of microorganisms often poses a challenge for pathologists. this can be done by use of nonspecific histochemical stains or by immunohistochemical analysis, but specific antibodies are often not available. we describe a novel method for the detection of microorganisms in valve specimens from patients with infective endocarditis.	2006	16703515
bartonella spp. in pets and effect on human health.	among the many mammals infected with bartonella spp., pets represent a large reservoir for human infection because most bartonella spp. infecting them are zoonotic. cats are the main reservoir for bartonella henselae, b. clarridgeiae, and b. koehlerae. dogs can be infected with b. vinsonii subsp. berkhoffii, b. henselae, b. clarridgeiae, b. washoensis, b. elizabethae, and b. quintana. the role of dogs as an important reservoir of bartonella spp. is less clear than for cats because domestic dogs ...	2006	16704774
new insight into the diagnosis of fastidious bacterial endocarditis.	sterile blood cultures are noted in one third of patients with infectious endocarditis. although in half of cases this is due to previous antibiotic therapy, in the other half, the aetiology of culture-negative endocarditis is intracellular bacteria such as coxiella burnetii or fastidious growing bacteria. although it was previously considered that the prevalence of such organisms was identical throughout the world, recent investigations on bartonella endocarditis clearly showed that the aetiolo ...	2006	16706783
environmental signals generate a differential and coordinated expression of the heme receptor gene family of bartonella quintana.	of all bacteria, bartonella quintana has the highest reported in vitro hemin requirement, yet an explanation for this remains elusive. to produce diseases such as trench fever, endocarditis, and bacillary angiomatosis, b. quintana must survive and replicate in the disparate environments of the pediculus humanus corporis (body louse) gut and the human vasculature. we previously identified a five-member family of hemin binding proteins (hbps) synthesized by b. quintana that bind hemin on the outer ...	2006	16714552
binding of bartonella henselae to extracellular molecules: identification of potential adhesins.	bartonella henselae, the etiologic agent of cat scratch disease, bacillary angiomatosis and other clinical syndromes initiates infection through a trauma or wound to the skin suggesting involvement of extracellular matrix molecules. we have demonstrated in this study that b. henselae bound strongly fibronectin, collagen ix and x, but comparatively less laminin and collagen iv. b. henselae bound primarily the n- and c-terminal heparin (hep-1 and hep-2, respectively) and the gelatin-binding domain ...	2006	16725305
louse-borne bacterial pathogens in lice (phthiraptera) of rodents and cattle from egypt.	we collected 1,023 lice, representing 5 species, from rats and domestic cattle throughout 13 governorates in egypt and tested these lice for anaplasma marginale, bartonella spp., brucella spp., borrelia recurrentis, coxiella burnetii, francisella tularensis, and rickettsia spp. by pcr amplification and sequencing. five different louse-borne bacterial agents were detected in lice from rodents or cattle, including "bartonella rattimassiliensis", "b. phoceensis", and bartonella sp. near bartonella ...	2006	16729688
bartonella quintana coinfection with mycobacterium avium complex and cmv in an aids patient: case presentation.	as a greater number of hiv-infected patients survive despite profound immunodepression due to medical progress, we face complex infection with multiple agents in aids-patients.	2006	16732891
evaluation of the relationship between causative organisms and clinical characteristics of infective endocarditis in dogs: 71 cases (1992-2005).	to evaluate microbiologic findings in dogs with infective endocarditis (ie) and determine whether there were differences in clinical features of disease caused by different groups of infective agents.	2006	16740074
multifocal osteomyelitis in a child: a rare manifestation of cat scratch disease: a case report and systematic review of the literature.	we present a case of a 9-year-old immunocompetent girl who presented with pain in her left elbow and a painful swelling at her left clavicle. she had no lymphadenopathy or fever. four hot spots were seen at her left clavicle, proximal and distal left humerus and lumbo-sacral spine on a bone scan. a magnetic resonance imaging showed an inflammatory process with bone destruction at her clavicle. serological testing and polymerase chain reaction performed on a bone biopsy identified a bartonella he ...	2006	16751739
aetiological diagnosis of infective endocarditis by direct amplification of rrna genes from surgically removed valve tissue. an 11-year experience in a finnish teaching hospital.	the aetiology of infective endocarditis (ie) can be determined directly from surgically removed valve tissue using broad-range bacterial rdna polymerase chain reaction (pcr) followed by sequencing. we sought to assess the value of this methodology in a routine clinical setting.	2006	16754257
[submandibular tumor].		2006	16756901
isolation of bartonella henselae dna from the peripheral blood of a patient with cat scratch disease up to 4 months after the cat scratch injury.	we report the case of a girl with cervical lymphadenitis and a persistent primary lesion of cat scratch disease (csd). bartonella henselae dna was isolated from plasma samples collected 3 and 4 months after the cat scratch, indicating that recurrent and long-term shedding of bartonella dna into peripheral blood may occur in typical csd.	2006	16757642
etiological diagnosis of blood-culture-negative endocarditis.		2006	16762253
[endocarditis due to bartonella spp. three new clinical cases and spanish literature review].	infections by bartonella spp. include a wide spectrum of emerging and re-emerging infectious diseases, such as culture-negative endocarditis.	2006	16762254
possible role of tick-borne infection in "cat-scratch disease": comment on the article by giladi et al.		2006	16802385
borrelia, coxiella, and rickettsia in carios capensis (acari: argasidae) from a brown pelican (pelecanus occidentalis) rookery in south carolina, usa.	argasid ticks are vectors of viral and bacterial agents of humans and animals. carios capensis, a tick of seabirds, infests the nests of brown pelicans, pelecanus occidentalis, and other ground nesting birds along the coast of south carolina. this tick is associated with pelican nest abandonment and could pose a threat to humans visiting pelican rookeries if visitors are exposed to ticks harboring infectious agents. we collected ticks from a pelican rookery on deveaux bank, south carolina and sc ...	2006	16821092
multispacer typing to study the genotypic distribution of bartonella henselae populations.	bartonella henselae, a worldwide fastidious bacterium, has a feline reservoir and is pathogenic for humans. however, the relationship between human and cat isolates of b. henselae, as well as its population dynamics and geographic heterogeneity, is not fully understood, in part because of the absence of appropriate typing methods. multilocus sequence typing (mlst), the most discriminatory genotyping method for b. henselae, identified seven genotypes and suggested that human isolates arose from a ...	2006	16825371
disseminated bartonella infection following liver transplantation.	bartonella henselae has not only been identified as the causative agent of cat scratch disease, but it is also associated with other significant infectious syndromes in the immunocompromised population. we describe two cases of b. henselae associated diseases in liver transplant recipients who both had contact with cats. the first recipient developed localized skin manifestation of bacillary angiomatosis in association with granulomatous hepatitis. he tested positive for immunoglobulin g (igg) a ...	2006	16827686
rodent-associated bartonella febrile illness, southwestern united states.	serum specimens from 114 patients hospitalized with a febrile illness were tested with an indirect immunofluorescence assay (ifa) using bartonella antigens prepared from 6 species of sigmodontine rodents and 3 known human bartonella pathogens: b. henselae, b. quintana, and b. elizabethae. acute- and convalescent-phase serum samples from 5 of these patients showed seroconversion with an ifa titer >512 to rodent-associated bartonella antigens. the highest titer was against antigen derived from the ...	2006	16836824
surveillance of egyptian fleas for agents of public health significance: anaplasma, bartonella, coxiella, ehrlichia, rickettsia, and yersinia pestis.	serologic surveys in egypt have documented human and animal exposure to vector-borne bacterial pathogens, but the presence and distribution of these agents in arthropods has not been determined. between july 2002 and july 2003, fleas were collected from 221 mammals trapped in 17 cities throughout egypt. a total of 987 fleas were collected, representing four species (ctenocephalides felis, echidnophaga gallinacea, leptopsylla segnis, and xenopsylla cheopis); 899 of these fleas were x. cheopis fro ...	2006	16837707
production of recombinant bartonella henselae 17-kda protein for antibody-capture enzyme-linked immunosorbent assay.	the bartonella henselae 17-kda protein was expressed in a prokaryotic expression system as a histidine-tagged fusion protein and was purified. the target gene was cloned into a recombinant expression construct, ptri-17kd. the expressed protein was purified to near homogeneity by a nickel-agarose column chromatography. protein recovery was estimated to be 2.9 mg from 100 ml of bacterial culture. the purified 17-kda protein was recognized by serum from patients infected with b. henselae and barton ...	2006	16490335
bartonella quintana characteristics and clinical management.	bartonella quintana, a pathogen that is restricted to human hosts and louse vectors, was first characterized as the agent of trench fever. the disease was described in 1915 on the basis of natural and experimental infections in soldiers. it is now recognized as a reemerging pathogen among homeless populations in cities in the united states and europe and is responsible for a wide spectrum of conditions, including chronic bacteremia, endocarditis, and bacillary angiomatosis. diagnosis is based on ...	2006	16494745
epidemiological studies on bartonella quintana infections among homeless people in tokyo, japan.	in an epidemiological investigation of trench fever in japan, we compared the seroprevalence of bartonella quintana in homeless people and in the general population. in homeless rescue outreach programs held in tokyo from may 2001 to march 2003, 151 blood samples were taken from non-hospitalized homeless people. the prevalence of igm and igg antibodies against b. quintana in these people was compared with that in 200 healthy blood donors using a commercially available indirect fluorescent antibo ...	2006	16495631
localized bacillary angiomatosis in the oral cavity: observations about a neoplasm with atypical behavior. description of a case and review of the literature.	bacillary angiomatosis is a rather frequent infectious pathology appearing mainly in the skin but can also affect the liver, spleen, heart, bones, lungs, muscles, central nervous system and other organs. the localization of the lesion in the oral cavity is rather rare, as it is evident in the literature. bacillary angiomatosis can be clinically similar to the kaposi's sarcoma and histologically confused with angiosarcoma, epitheloid hemangioma and pyogenic granuloma. a case of bacillary angiomat ...	2006	16495874
first molecular evidence of bartonella quintana in pediculus humanus capitis (phthiraptera: pediculidae), collected from nepalese children.	trench fever is a body louse-borne disease caused by bartonella quintana brenner. the recent status of louse infestation in nepalese children is not well known. we collected head and body lice, pediculus humanus capitis de geer and pediculus humanus humanus l., respectively, from 30 children, including 11 cases of double infestation with both head and body lice. detection of b. quintana in both louse species identified was carried out by polymerase chain reaction (pcr). pcr products with b. quin ...	2006	16506456
bartonella henselae bacilli detected in vitrectomy aspirates in a patient with massive vitreous opacity with total retinal detachment.	to present endophthalmitis-like massive vitreous opacity as a manifestation of cat-scratch disease.	2006	16507491
do dogs harbour risk factors for human breast cancer?	we ask consulting patients regularly whether they keep pets in order to identify zoonotic factors. it became apparent that patients with breast carcinoma (n=69) owned significantly more often dogs but not cats compared to age matched female controls. we compared the frequencies of dog and pet ownership with data from public available statistics on women (n=1320) of the same age group in bavaria. the most striking result was that more than twice the number of patients kept dogs permanently in the ...	2006	16516398
brucella outer membrane protein omp31 is a haemin-binding protein.	the expression of haemin-binding proteins (hbps) in the outer membrane is one of the strategies used by gram-negative bacteria to obtain iron from the host. no hbp has been described in brucella spp. we investigated whether omp31, an outer membrane protein from brucella with homology to hbps from bartonella quintana, is an hbp. soluble recombinant omp31 bound specifically to haemin-agarose, while an unrelated brucella protein (sura) did not. a similar experiment showed that native omp31 found in ...	2006	16517201
molecular detection of bartonella quintana, b. elizabethae, b. koehlerae, b. doshiae, b. taylorii, and rickettsia felis in rodent fleas collected in kabul, afghanistan.	the prevalences of bartonella spp. and rickettsia spp. were investigated using molecular methods in 77 rodent fleas collected in november 2002 by the french forces detachment in kabul, afghanistan. overall, bartonella dna was detected in 15.5% of gerbil fleas and 40.5% of rat fleas, whereas rickettsia felis was found in 9% of gerbil fleas. we described for the first time in this country bartonella quintana, b. koehlerae, b. taylorii, and rickettsia felis in fleas from the gerbil species meriones ...	2006	16525103
type iv secretion systems and their effectors in bacterial pathogenesis.	type iv secretion systems (t4sss) are membrane-associated transporter complexes used by various bacteria to deliver substrate molecules to a wide range of target cells. t4sss are involved in horizontal dna transfer to other bacteria and eukaryotic cells, in dna uptake from or release into the extracellular milieu, in toxin secretion and in the injection of virulence factors into eukaryotic host target cells by several mammalian pathogens. rapid progress has been made towards defining the structu ...	2006	16529981