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developmental origins of moral masochism: a failure-to-thrive toddler's interactions with mother.the case of a toddler diagnosed as failure to thrive with an unusual history of "accidents" illustrates the circumstances which may account for the infant's intense attachment to a sadistic love object and lead to moral masochism in adulthood. the observed behaviors of the toddler in interaction with mother show the pathological consequences of the infant's openness to influences of the social environment. he becomes attuned to his mother's wishes and performs behaviors which successfully evoke ...20133676888
the italian national research council and hospital infections.in 1983 the italian national research council funded a project specifically focused on hospital infection control. the main objectives were to describe the epidemiological pattern of hospital-acquired infections; to evaluate different control measures and preventive strategies. the general frame and research issues of the project are discussed in this paper. the main research areas of the project were: urinary tract infections; infections in surgery; intensive care units/high risk patients; micr ...20133607928
[aerobic capacity estimated from heart rate under submaximal load: an indicator of complaints, personality, personal activity and social status].in 34 young females (mean: 21 years, range 18-30), working with vdts, maximum aerobic capacity (mac) was estimated by use of a cycle ergometer and astrand's nomogram. mac correlated with musculoskeletal complaints (-.29, p less than .05), eye complaints (-.36, p less than .05), personal activity (.38, p less than .05), personality dimensions: "imperturbability" (.41, p less than .01), "wellbeing" (.31, p less than .05), "traditionalism" (-.45, p less than .01) and "psychophysic constitution" (al ...20133811609
[realities in the diagnosis of bronchial carcinoma in clinical and general practice].in 100 patients with lung cancer the diagnostic procedure, results of clinical staging and therapeutic sequelae were analysed retrospectively and compared to a similar group of patients 10 years previously. during the 10 years' interval there was a remarkable shortening of the time between the onset of symptoms and the diagnosis, from 20 weeks to the present 10 weeks. despite this acceleration the number of patients undergoing curative surgical resection increased only from 15 to 17%. inoperabil ...20133672088
[health administration: management in changing society and in age of definition]. 20133650543
colonic carcinoma metastasizing to the choroid. 20133709825
tailoring genes for crop improvement. an agricultural perspective. 20133593177
the liver and metabolic bone disease. 20133837818
[clinical and myopathological findings in polymyositis].the present report analyzed a clinical and myopathological findings of 60 patients defined for the diagnosis of polymyositis. patients were classified in four groups according to the system walton and adams. forty five per cent were classified as group i, 28.3 per cent as group ii, 15.0 per cent as group iii and 11.7 per cent as group iv. seven patients were associated with a malignant neoplasm and all of them were over fifty. the primary growth was carcinoma of the lung in 2, of the prostate, o ...20133675929
"normal" blood pressure in chimpanzees.during routine physical examination under ketamine sedation, 140 blood pressure measurements from male and 170 from female chimpanzees were derived over a period of 3 yr. statistical analysis revealed generally no differences between the sexes but significant increases of both systolic and diastolic blood pressure with increasing age. the data obtained may constitute a useful management tool with which to identify hypertensive chimpanzees and suggests that this animal species could be a model fo ...20133694667
phantom testing of peripheral artery. absolute blood flow measurement with digital arteriography.a technique to measure absolute arterial blood flow in ml/min has been developed utilizing software programs with high-speed digital recording for the off-line analysis of intra-arterial injections of contrast medium. measurements of pulsatile flow in a phantom for a physical flow model showed that calculations with the final upgrades were within 10% of known flow, using a recording rate of 30 frames/s, a diameter tubing of 5 mm, and flow rates of 300 to 400 ml/min. quantitative absolute flow of ...20133886590
alcohol-related symptoms. 20133877581
treatment of acute abscesses by incision, curettage and primary suture without antibiotics: a controlled clinical trial.one hundred and thirty-seven consecutive acute superficial abscesses were randomly allocated to incision, curettage and primary suture without parenteral antibiotics (64 cases) or to conventional treatment with incision, drainage and dressings (73 cases). wound healing was faster; 7.0 days: 25.1 days (p less than 0.01, wilcoxon's rank sum test), the number of hospital visits was smaller; 3.8 visits: 11.1 visits (p less than 0.01) and the time off work was shorter; 4.0 days: 14.1 days (p less tha ...20133881155
vasodilator drugs in patients with chronic ischaemic heart failure.the effects of intravenous nitroglycerin (ntg), trimetaphan (tmp), and phentolamine (ptl) on pulmonary artery diastolic pressure (padp), systemic arterial pressure (sap), cardiac index (ci) and systemic vascular resistance (svr) in patients (12 in each treated group) with chronic ischaemic heart failure are analyzed. each group was divided into two subgroups according to the initial padp taking into account the mean value in the whole group. a significant decrease in padp (by 40%; p less than 0. ...20133935374
[the significance of abnormal qrs amplitudes in the electrocardiogram]. 20134222444
structure and organization of immunoglobulin vh genes in heterodontus, a phylogenetically primitive shark. 20133936736
structure and organization of immunoglobulin vh genes in heterodontus, a phylogenetically primitive shark. 20133936736
emotions and obesity among mexican-american children.the purpose of this study was to determine what relationships, if any, existed between high levels of body fat and emotional motivations for eating among mexican-american children. subjects (n = 177) were drawn from children aged 10-14 years who participated in the national youth sports program in the lower rio grande valley region of texas. data were gathered through a self-report instrument dealing with emotional motivators involved in eating and through individual anthropometric measurements. ...20133845258
associated postural adjustments in parkinson's disease.postural activity in muscles of the back and legs associated with voluntary forward elevation of the arm at the shoulder with the subject standing was examined in 14 patients with parkinson's disease and nine normal individuals. the time of onset of emg activity in each of the postural muscles, relative to the onset of activity in the prime mover, was the same for both patient and normal groups and did not depend on load. the amplitude of the emg bursts and their frequency of occurrence, was les ...20133806114
[urinary acidic mucopolysaccharides in normal individuals and patients with hurler's syndrome]. 20134240974
[urinary acidic mucopolysaccharides in normal individuals and patients with hurler's syndrome]. 20134240974
wheelchair use by ambulatory nursing home residents.in a survey of wheelchair use in two nursing homes, the authors found a substantial number of cognitively intact nursing home residents who walked, but who also used a wheelchair. using chart review and interviews with the residents, it was found that multiple physical factors, including pain, strength, endurance, vision and balance, and multiple social and environmental factors, including the fear of falling, were related to the resident's decision to use a wheelchair as an alternative means of ...20133782699
[acute myocardial infarction during fasting]. 20133765158
a computer model of neck muscle endurance and fatigue as a function of helmet loading.a series of experiments were conducted in which the neck muscles of volunteer subjects were dynamically and statistically loaded by systematic variations of twenty-four headgear configurations consisting of eight different centers-of-gravity (cgs) times three different weights. six subjects would rotate their heads laterally (from side-to-side) for 30 min with each of the headgear loading combinations. immediately thereafter, the subject would position his head in an isometric head dynamometer a ...20133754195
mri: applications in soft tissue anatomy. 20133739418
the cell-to-cell channel of gap junctions. 20133815521
the dorsal approach for internal fixation of fractures of the lateral malleolus.anatomic reduction and internal fixation of the lateral malleolus are the key to management of ankle fractures. the usual application of one-third tubular plates to the lateral surface of the distal fibula has certain disadvantages. in selected cases we prefer a dorsal approach to the lateral malleolus. indications, technique and experience are discussed.20133725110
measurement of sebum output using a lipid absorbent tape.a sebum absorbent tape is introduced as a reproducible and convenient method for estimation of sebaceous gland output. we have tested the reproducibility of this method by serial measurements of sebum excretion rates (ser) of 10 individuals over a 6-week period, and in addition we have correlated this method with the conventional hexane extraction technique. the sebum absorbent tapes gave consistent values for the sers, and within subjects variation over the 6-week period was statistically nonsi ...20133734473
self-resistance in actinomycetes producing inhibitors of rna polymerase. 20133710921
[effect of irradiation on the size of isolated liver mitochondria].the role of external effects in the time course of changes in hepatocyte mitochondria size was studied. suspensions of mitochondria isolated from the liver of inbred mice were investigated at various periods after gamma irradiation. statistical analysis of changes in the size of isolated mitochondria has shown that irradiation leads to an increase in mitochondria size, reflected in the distribution pattern. the changes may be related to the functional activity, and distribution shifts may be reg ...20133697486
metabolism during the postinjury period. 20134906806
error rates in prenatal cystic fibrosis diagnosis. 20133713828
clinical, morphologic, biochemical, and immunohistochemical aspects of dialysis-associated amyloidosis. 20133675951
[diagnostic usefulness in the measurement of crp by lpia-300]. 20133669370
[clinical considerations on post-traumatic vasomotor disorders (sudeck's atrophy)]. 20134170453
[treatment with cis-platin. retinal toxic effects]. 20133603502
[developmental course of opiate-dependent patients over 6 years (1979-1985)].drug addicts of the basle area have been analysed during 6 years in connexion with the national research programme 'social integration' and also a following project. the results are contradictory to the pessimism which exists against changes of development of drug addicts.20133630400
[a simple method of measuring serum sialic acid level as a neoplasm marker]. 20133684762
long term effect of gynecologic age on somatic growth of children.a sample of new orleans black primiparous women under 25 years of age, participants in the national collaborative perinatal study, and their singleton children were studied to determine the relationship of maternal gestational age (gea), gynecologic age (gya) and size for gea (s/gea) on the child's growth over seven years observation. early childbearers (less than 4 years gya) had a somewhat higher proportion of preterm births than the late group (greater than or equal to 4 yrs gya). a repeated ...20133624531
isolated perfused ambystoma proximal tubule: hydrodynamics modulates ultrastructure.a method using a pressure-sensing servo-pipette is described for measuring downstream transepithelial pressure within isolated renal tubules perfused at flow rates designed to keep luminal solution composition constant. the hydrodynamics of in vitro microperfusion of isolated proximal tubules of ambystoma tigrinum was varied and different states of transepithelial hydrostatic pressure difference, axial tubule flow, and transepithelial transport were correlated with epithelial ultrastructure. tub ...20133591955
you and malpractice stress: v. a death in the family. 20133559428
modifications of plasma lipoproteins after lipase activation in patients with chylomicronemia.lipoprotein lipase (lpl) and hepatic lipase (hl) are enzymatic activities involved in lipoprotein metabolism. the purpose of this study was to analyze the physicochemical modifications of plasma lipoproteins produced by lpl activation in two patients with apoc-ii deficiency syndrome and by hl activation in two patients with lpl deficiency. lpl activation was achieved by the infusion of normal plasma containing apoc-ii and hl was released by the injection of heparin. lipoproteins were analyzed by ...20133559389
spontaneous passage of renal calculi through nephrocutaneous fistula due to calculous pyelonephritis. 20133567499
[sketches on the history of the training of paramedical personnel in russia]. 20133553835
[lenin's "iskra" on the role and health status of adolescent workers in russia]. 20133523773
correlation of double-contrast high-density barium enema, colonoscopy, and histology in children with special attention to disparities.colonoscopic and double-contrast high-density barium enema (dcbe) findings were correlated in 68 patients (39 boys and 29 girls) aged 6 months to 18 years (mean 11.6 years) evaluated over a 24-month period. there was excellent correlation in 53 patients (78.0%) and good correlation in another 3 (4.4%) who had identical diagnoses and only slightly differing extent of disease reported. in 2 of these, dcbe showed more extensive disease, confirmed histologically in 1. distal colitis seen on colonosc ...20133725447
the role of the paediatric endoscopist.the role of the paediatric endoscopist has been a changing one. when i first commenced at the royal alexandra hospital for children in sydney 25 years ago, paediatric endoscopy of the upper respiratory tract and oesophagus was a limited and poorly defined field. most endoscopic examinations were requested, often reluctantly, by a paediatrician in the investigation of stridor. in fact my first consultation involved instructions to perform a laryngoscopy, but not a bronchoscopy and general anaesth ...20133805880
[troublesome teeth in ambulatory care]. 20133859858
[troublesome teeth in ambulatory care]. 20133859858
[in vitro fertilization in reduced male fertility]. 20134029742
[factors determining the severity of traumatic lesions of the popliteal artery]. 20134073583
[analysis of hba1 is necessary in the routine check-up of patients with type 2 diabetes mellitus]. 20133807486
[conservative treatment in splenic injuries. results of a follow-up in patients with autotransplants]. 20133725057
projecting mri use: key to scanner purchases. 20133679125
peroxidase-based colorimetric determination of l-ascorbic acid. 20133592224
[significance of internalization and externalization in normal development]. 20133685240
[sleep in children]. 20134321695
temporal lobe epilepsy with schizophrenia.i have reported a case of coexisting schizophrenia and complex partial seizures to augment earlier reports of schizophrenia and psychomotor seizures masquerading as one another. i have attempted to clarify the differential diagnosis and reinforce the need to do thorough studies in such cases.20133616704
relation between nasal/voice accelerometric values and interval estimates of hypernasality.nasal/throat accelerometric ratios were obtained from 12 hypernasal and three normal children. the accelerometric voltages comprising the ratio were analogs of nasal and anterior-neck tissue vibrations. audio recordings, which were obtained simultaneously with the accelerometric voltages, were later judged for hypernasality using an equal-appearing-interval (eai) scale. high correlations were evident between accelerometric and eai values when a stimulus sentence contained obstruents, semivowels, ...20133863721
[rates of incidence and prevalence of various neuromuscular diseases in the udine and pordenone provinces]. 20133796514
[surgical wound infection after spondylodesis with instrumentation]. 20133811754
non-stochastic effects: compatibility with present icrp recommendations.the present recommendations of the icrp (international commission on radiological protection) are almost entirely based on 'stochastic effects' of ionizing radiation, i.e. cancer induction and heritable effects. in a recent report the compatibility of present recommendations with non-stochastic effects has been considered. the present paper is a summary of these findings.20133874183
non-stochastic effects: compatibility with present icrp recommendations.the present recommendations of the icrp (international commission on radiological protection) are almost entirely based on 'stochastic effects' of ionizing radiation, i.e. cancer induction and heritable effects. in a recent report the compatibility of present recommendations with non-stochastic effects has been considered. the present paper is a summary of these findings.20133874183
effects of sustained intravenous diltiazem infusion in healthy persons.a 3-stage infusion of diltiazem was tested in 8 subjects for up to 48 hours: a bolus injection (10 mg over 3 minutes), a rapid loading infusion (20 mg over 30 minutes) and a maintenance infusion (10 mg/hour to the end of the study). this regimen produced stable median plasma diltiazem concentrations of approximately 150 ng/ml. the median half-life of elimination for diltiazem was 206 minutes (range 144 to 452) and median total clearance was 980 ml/min (range 665 to 1,907). the pr interval length ...20133776858
radiation-induced stenosis of the left main coronary artery.a case of isolated left main coronary artery stenosis ten years following irradiation of the mediastinum for hodgkin's disease is presented. aortic biopsies were consistent with changes suggestive of radiation arteritis.20133731265
[features of the clinico-roentgenologic picture of acute pneumonia in chronic alcoholics]. 20133705450
an illusion of reversed direction in hyperopes.if a subject who is sufficiently farsighted removes his corrective, positive, lenses and looks with one eye from a distance of one or a few meters, at a small lighted area such as the (continuously "on") indicator light of an electric toothbrush, razor, or smoke detector, and if a small object such as a pin is then moved slowly from above to below the subject's eyes (in a plane close to the eye), the subject will perceive the object moving normally from above to below until it encroaches on his ...20133696931
diagnosis formulation by residents and physicians at different levels of experience. 20133783644
combined extracapsular cataract extraction and posterior chamber intraocular lens implantation and trabeculectomy.in a retrospective study in a consecutive series of operations performed by the author, 13 eyes in 10 patients underwent combined extracapsular cataract extraction, posterior chamber intraocular lens (iol) implantation and trabeculectomy. all had significant cataract and chronic open-angle glaucoma with field loss, uncontrolled or marginally controlled on maximum tolerable medical treatment. all had a postoperative reduction in intraocular pressure. significant complications included malignant g ...20133675912
haemothorax in the course of chickenpox. 20133787528
mucosa protectives: sucralfate and colloidal bismuth subcitrate in peptic ulcer disease.mucosa protective drugs are thought to have an important role in the treatment of both duodenal (du) and gastric ulcer (gu) disease by means of correcting the disturbed defensive factors. sucralfate as well as colloidal bismuth subcitrate (cbs) form a layer on the ulcer base and in this way protect the ulcer from acid, peptic activity and bile. in duodenal ulcer sucralfate and cbs have shown a significant difference in healing rate compared with placebo. the same holds true for gastric ulcer. wh ...20133660894
time-resolved x-ray diffraction studies of the b in equilibrium d structural transition in the dna double helix.because of the relation between topology and function, there has been much interest in the structural transitions of the various conformations of dna polymers. the x-ray fiber diffraction analysis system at the daresbury synchrotron radiation source was used to study the reversible transition between the b and d forms of the synthetic dna poly[d(a-t)].poly[d(a-t)]. the gradual progression of conformations between these two forms indicates that the dna double helix does not undergo a change of ha ...20133726529
[vesico-uterine fistulas]. 20133623249
enzymatic procedure for the synthesis of 11-deoxyprostaglandins. 20133664795
[large-savings--both in economics and humans]. 20133647642
the cocaine epidemic: proof of the poison. 20133641046
[decreased activity of the renal vasodilation and natriuretic system in normotensive men with a familial history of essential hypertension and decreased na+, k+-cotransport in erythrocytes]. 20133536113
electrotransfer of the epinecidin-1 gene into skeletal muscle enhances the antibacterial and immunomodulatory functions of a marine fish, grouper (epinephelus coioides).electrotransfer of plasmid dna into skeletal muscle is a common non-viral delivery system for the study of gene function and for gene therapy. however, the effects of epinecidin-1 (epi) on bacterial growth and immune system modulation following its electrotransfer into the muscle of grouper (epinephelus coioides), a marine fish species, have not been addressed. in this study, pcmv-gfp-epi plasmid was electroporated into grouper muscle, and its effect on subsequent infection with vibrio vulnificu ...201323973381
an improved method for quantification of vibrio vulnificus in oysters.chromogenic agar was compared with the fda recommended cellobiose-colistin agar for assessment of vibrio vulnificus in oysters. a two-step culture confirmation method was also evaluated. the inclusion of ca gave a 33% increase in the detection rate and the two-step culture confirmation eliminated 62.5% of false positives.201323933605
luxr homologue smcr is essential for vibrio vulnificus pathogenesis and biofilm detachment, and its expression is induced by host cells.quorum sensing is a cell-to-cell communication system known to control many bacterial processes. in the present study, the functions of quorum sensing in the pathogenesis of vibrio vulnificus, a food-borne pathogen, were assessed by evaluating the virulence of a mutant deficient in smcr, a quorum-sensing regulator and homologue of luxr. when biofilms were used as an inoculum, the smcr mutant was impaired in virulence and colonization capacity in the infection of mice. the lack of smcr also resul ...201323897607
retrospective analysis of epidemiological aspects of vibrio vulnificus infections in korea in 2001-2010.in this study, we performed a retrospective, quantitative analysis of the epidemiological aspects and risk factors of vibrio vulnificus infections in korea from 2001 to 2010. in a total of 588 v. vulnificus infection cases (prevalence rate, 0.12 cases/100,000 persons), 285 were fatal (case-fatality rate [cfr], 48.5%). males were more significantly infected by v. vulnificus than females (86.1% versus 13.9%; p < 0.01), and a higher incidence of v. vulnificus infections was observed in people aged ...201323883847
impact of 2003 state regulation on raw oyster-associated vibrio vulnificus illnesses and deaths, california, usa.us vibriosis rates have increased since 1996, and many vibrio vulnificus infections are fatal. in april 2003, california implemented a regulation restricting the sale of raw oysters harvested from the gulf of mexico during april 1-october 31, unless they were processed to reduce v. vulnificus to nondetectable levels. we analyzed california cases of v. vulnificus infection before and after the regulation's implementation and compared case data with data from other states. the annual number of rep ...201323876744
seasonality, clinical types and prognostic factors of vibrio vulnificus infection.vibrio vulnificus infection, an uncommon but life-threatening illness, manifests as two main types, primary septicemia and primary wound infections.  little information regarding the seasonality of v. vulnificus infections in tropical areas and prognostic factors of primary v. vulnificus wound infections is available.201323857388
three-state mechanism couples ligand and temperature sensing in riboswitches.riboswitches are cis-acting gene-regulatory rna elements that can function at the level of transcription, translation and rna cleavage. the commonly accepted molecular mechanism for riboswitch function proposes a ligand-dependent conformational switch between two mutually exclusive states. according to this mechanism, ligand binding to an aptamer domain induces an allosteric conformational switch of an expression platform, leading to activation or repression of ligand-related gene expression. ho ...201323842498
comparison of plating media for recovery of total and virulent genotypes of vibrio vulnificus in u.s. market oysters.vibrio vulnificus is the leading cause of seafood associated mortality in the united states and is generally associated with consumption of raw oysters. two genetic markers have emerged as indicators of strain virulence, 16s rdna type b (rrnb) and virulence correlated gene type c (vcgc). while much is known about the distribution of v. vulnificus in oysters, a limited number of studies have addressed the more virulent subtypes. therefore, the goals of this study were to (1) determine the suitabi ...201324184610
solution structure and dynamics of c-terminal regulatory domain of vibrio vulnificus extracellular metalloprotease.an extracellular metalloprotease (vep) secreted by vibrio vulnificus atcc29307 is a 45-kda proteolytic enzyme that has prothrombin activation and fibrinolytic activities during bacterial infection. the action of vep could result in clotting that could serve to protect the bacteria from the host defense machinery. very recently, we showed that the c-terminal propeptide (c-ter100), which is unique to vep, is involved in regulation of vep activity. to understand the structural basis of this functio ...201323228665
resistance to environmental stresses by vibrio vulnificus in the viable but nonculturable state.vibrio vulnificus is responsible for 95% of all seafood-associated fatalities in the united states. when water temperatures drop below c. 13 °c, the cells enter into the viable but nonculturable (vbnc) state wherein they are unable to grow on routine media but retain viability and the ability to return to the culturable state. the aim of this study was to determine whether v. vulnificus cells in the vbnc state are protected against a variety of potentially lethal challenges (heat, oxidative, osm ...201323228034
a bacterial rtx toxin causes programmed necrotic cell death through calcium-mediated mitochondrial dysfunction.vibrio vulnificus, a halophilic estuarine bacterium causing fatal septicemia and necrotic wound infection, is highly cytotoxic to eukaryotic cells. we have reported that rtxa1 toxin kills host cells only after they come into contact with bacteria and plays an essential role in the pathogenesis of v. vulnificus. this study was performed to elucidate the mechanism by which the rtxa1 toxin mediates the death of hela cells. by using confocal microscopy and immunoblot analysis, we show that the 501-k ...201323225896
bullous skin lesions in vibrio vulnificus infection. 201323178913
an assay to screen bacterial adhesion to mucus biomolecules.to develop an assay for rapid screening of bacterial adhesion to various groups of biomolecules present in fish mucus.201323020180
martx of vibrio vulnificus biotype 2 is a virulence and survival factor.vibrio vulnificus biotype 2 is a polyphyletic group whose virulence for fish relies on a plasmid. this plasmid contains an rtxa gene duplicated in the small chromosome that encodes a martx (multifunctional, autoprocessing repeats-in-toxin) unique within the species in domain structure (martx type iii). to discover the role of this toxin in the fitness of this biotype in the fish-farming environment, single- and double-knockout mutants were isolated from a zoonotic strain and analysed in a series ...201322943291
role of periplasmic binding proteins, fatb and vatd, in the vulnibactin utilization system of vibrio vulnificus m2799.vibrio vulnificus, an opportunistic marine bacterium that causes a serious, often fatal, infection in humans, requires iron for its pathogenesis. this bacterium uses iron from the environment via the vulnibactin-mediated-iron-uptake system. in this study, we constructed the deletion mutants of the genes encoding the proteins involved in the vulnibactin-mediated-iron-uptake system, isochorismate synthase (ics), vulnibactin utilization protein (vuub), periplasmic ferric-vulnibactin binding protein ...201324135791
prevalence and genetic characterization of vibrio vulnificus in raw seafood and seawater in malaysia.vibrio vulnificus is a highly invasive human pathogen that exists naturally in estuarine environment and coastal waters. in this study, we used different pcr assays to detect v. vulnificus in 260 seafood and 80 seawater samples. v. vulnificus was present in about 34 (13%) of the 260 seafood samples and 18 (23%) of the 80 seawater samples. repetitive extragenic palindromic pcr (rep-pcr) and enterobacterial repetitive intergenic consensus pcr (eric-pcr) were applied to subtype the v. vulnificus is ...201324112583
[preparation of monoclonal antibodies against flagellin core protein of vibrio vulnificus and its application in testing vibrio vulnificus from food products].to prepare the monoclonal antibodies (mabs) against flagellin core protein of vibrio (v.) vulnificus and establish the double-antibody sandwich elisa for testing v. vulnificus from food products.201324103267
role of capsular polysaccharide (cps) in biofilm formation and regulation of cps production by quorum-sensing in vibrio vulnificus.extracellular polysaccharides, such as lipopolysaccharide and loosely associated exopolysaccharides, are essential for vibrio vulnificus to form biofilms. the role of another major component of the v. vulnificus extracellular matrix, capsular polysaccharide (cps), which contributes to colony opacity, has been characterized in biofilm formation. a cps-deficient mutant, whose wbpp gene encoding udp-glcnac c4-epimerase was knocked out, formed significantly more biofilm than wild type, due to increa ...201324102883
transcriptomic analysis of genes modulated by cyclo(l-phenylalanine-l-proline) in vibrio vulnificus.diketopiperazine is produced by various organisms, including bacteria, fungi, and animals, and has been suggested as a novel signal molecule involved in the modulation of genes with various biological functions. vibrio vulnificus, which causes septicemia in humans, produces cyclo(l-phenylalanine-l-proline) (cfp). to understand the biological roles of cfp, the effect of the compound on the expression of the total mrna in v. vulnificus was assessed by nextgeneration sequencing. based on the transc ...201324100622
antibacterial activity of biosecur(®) citrus extract surface cleaner against vibrio vulnificus.this study evaluated the antibacterial activity of biosecur(®) citrus extract surface cleaner against vibrio vulnificus using plate count method. two concentrations, 0.5% and 2% of biosecur(®) surface cleaner were plated on vibrio vulnificus agar (vva) and tested for reduction of vibrio vulnificus. in order to investigate the lasting residual activity of biosecur(®), antibacterial activity tests were also performed at time intervals up to 2.5 h after biosecur(®) was plated on vva. biosecur(®) sh ...201324302976
molecular characterization and antibiotic susceptibility of vibrio vulnificus in retail shrimps in hangzhou, people's republic of china.vibrio vulnificus is a gram-negative bacterium that occurs naturally in estuarine and marine water and is associated with wound infections or septicemia related to the consumption of raw shellfish in humans. the molecular characteristics and antibiotic susceptibilities of v. vulnificus strains in shrimps from retail markets in hangzhou, people's republic of china, were investigated in this study. thirty-three samples were positive for v. vulnificus in 78 shrimp samples which were collected from ...201324290683
rapid emergency medicine score as a main predictor of mortality in vibrio vulnificus-related patients.vibrio vulnificus causes potentially life-threatening and rapidly progressing infections. therefore, the severity-of-illness assessment appears to be important for v vulnificus-infected patients at the time of admission. the aim of our study was to evaluate the performance of the severity-of-illness scoring model on admission in v vulnificus-infected patients.201323702054
a novel agar formulation for isolation and direct enumeration of vibrio vulnificus from oyster tissue.a new selective and differential medium, vibrio vulnificus x-gal (vvx), was developed for direct enumeration of v. vulnificus (vv) from oyster samples. this agar utilizes cellobiose and lactose as carbon sources, and the antibiotics colistin and polymyxin b as selective agents. hydrolysis of 5-bromo-4-chloro-3-indolyl- beta-d-galactopyranoside (x-gal), used in the agar as a lactose analog, produces an insoluble blue dye that makes lactose positive colonies easily distinguishable from any non-lac ...201323660708
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