Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
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| highly diverse recombining populations of vibrio cholerae and vibrio parahaemolyticus in french mediterranean coastal lagoons. | vibrio parahaemolyticus and vibrio cholerae are ubiquitous to estuarine and marine environments. these two species found in mediterranean coastal systems can induce infections in humans. environmental isolates of v. cholerae (n = 109) and v. parahaemolyticus (n = 89) sampled at different dates, stations and water salinities were investigated for virulence genes and by a multilocus sequence-based analysis (mlsa). v. cholerae isolates were all ctxa negative and only one isolate of v. parahaemolyti ... | 2015 | 26236294 |
| occurrence of vibrio parahaemolyticus, vibrio cholerae, and vibrio vulnificus in the aquacultural environments of taiwan. | the occurrence of vibrio parahaemolyticus, vibrio vulnificus, and vibrio cholerae in a total of 72 samples from six aquaculture ponds for groupers, milk fish, and tilapia in southern taiwan was examined by the membrane filtration and colony hybridization method. the halophilic v. parahaemolyticus was only recovered in seawater ponds, with a high isolation frequency of 86.1% and a mean density of 2.6 log cfu/g. v. cholerae was found in both the seawater and freshwater ponds but preferentially in ... | 2015 | 25951392 |
| dimeric wh2 repeats of vopf sequester actin monomers into non-nucleating linear string conformations: an x-ray scattering study. | vopf and vopl are highly similar virulence-factors of vibrio cholerae and vibrio parahaemolyticus respectively that disrupt the host's actin cytoskeleton, using a unique organization in dimerized wh2 repeats. association of dimerized wh2 domains with the barbed face of actin confers multifunctional activities to vopf in vitro, including g-actin sequestration and filament nucleation, barbed end tracking and uncapping. here, small angle x-ray scattering (saxs) measurements of complexes of vopf wit ... | 2015 | 25818509 |
| differential specificity of selective culture media for enumeration of pathogenic vibrios: advantages and limitations of multi-plating methods. | plating environmental samples on vibrio-selective chromogenic media is a commonly used technique that allows one to quickly estimate concentrations of putative vibrio pathogens or to isolate them for further study. although this approach is convenient, its usefulness depends directly on how well the procedure selects against false positives. we tested whether a chromogenic medium, chromagar vibrio (cav), used alone (single-plating) or in combination (double-plating) with a traditional medium thi ... | 2015 | 25602161 |
| draft genome sequence of non-vibrio parahaemolyticus acute hepatopancreatic necrosis disease strain kc13.17.5, isolated from diseased shrimp in vietnam. | a strain of vibrio (kc13.17.5) causing acute hepatopancreatic necrosis disease (ahpnd) in shrimp in northern vietnam was isolated. normally, ahpnd is caused by vibrio parahaemolyticus, but the genomic sequence of the strain indicated that it belonged to vibrio harveyi. the sequence data included plasmid-like sequences and putative virulence genes. | 2015 | 26383659 |
| development of vibrio spp. infection resistance related snp markers using multiplex snapshot genotyping method in the clam meretrix meretrix. | the clam meretrix meretrix is a commercially important mollusc species in the coastal areas of south and southeast asia. in the present study, large-scale snps were genotyped by the multiplex snapshot genotyping method among the stocks of m. meretrix with different vibrio spp. infection resistance profile. firstly, the autosnp software was applied to mine snps from m. meretrix transcriptome, and 323 snp loci (including 120 indels) located on 64 contigs were selected based on uniprot-go associati ... | 2015 | 25655323 |
| prevalence and characterisation of non-cholerae vibrio spp. in final effluents of wastewater treatment facilities in two districts of the eastern cape province of south africa: implications for public health. | vibrios and other enteric pathogens can be found in wastewater effluents of a healthy population. we assessed the prevalence of three non-cholerae vibrios in wastewater effluents of 14 wastewater treatment plants (wwtp) in chris hani and amathole district municipalities in the eastern cape province of south africa for a period of 12 months. with the exception of wwtp10 where presumptive vibrios were not detected in summer and spring, presumptive vibrios were detected in all seasons in other wwtp ... | 2015 | 25167817 |
| occurrence and antibiotic resistance of vibrio parahaemolyticus from shellfish in selangor, malaysia. | high consumer demand for shellfish has led to the need for large-scale, reliable shellfish supply through aquaculture or shellfish farming. however, bacterial infections which can spread rapidly among shellfish poses a major threat to this industry. shellfish farmers therefore often resort to extensive use of antibiotics, both prophylactically and therapeutically, in order to protect their stocks. the extensive use of antibiotics in aquaculture has been postulated to represent a major contributi ... | 2015 | 26697003 |
| characterization of hemocyanin from the mud crab scylla paramamosain and its expression analysis in different tissues, at various stages, and under vibrio parahaemolyticus infection. | hemocyanin is an important respiratory protein in many arthropod and mollusk species. here, four cdnas (sphc1, sphc2, sphc3, and sphc4), encoding distinct hemocyanin subunits from scylla paramamosain were cloned using est analyses and the rapid amplification of cdna ends. the four full-length cdna fragments (sphc1-4) were 2281, 2002, 2184, and 2069 bp, respectively, and they encoded four putative proteins (570-676 amino acids) with a molecular mass of ~65.0-76.8 kda. quantitative real-time pcr a ... | 2015 | 26681010 |
| investigation of reference genes in vibrio parahaemolyticus for gene expression analysis using quantitative rt-pcr. | vibrio parahaemolyticus is a significant human pathogen capable of causing foodborne gastroenteritis associated with the consumption of contaminated raw or undercooked seafood. quantitative rt-pcr (qrt-pcr) is a useful tool for studying gene expression in v. parahaemolyticus to characterize its virulence factors and understand the effect of environmental conditions on its pathogenicity. however, there is not a stable gene in v. parahaemolyticus that has been identified for use as a reference gen ... | 2015 | 26659406 |
| [etiologic characteristics of food-borne vibrio parahaemolyticus strains isolated in guangdong, 2014]. | to investigate the serotypes, antimicrobial resistance, virulence genes and molecular characteristics of food-borne vibrio parahaemolyticus isolated in guangdong province in 2014. | 2015 | 26850252 |
| acute hepatopancreatic necrosis disease (ahpnd) outbreaks in penaeus vannamei and p. monodon cultured in the philippines. | acute hepatopancreatic necrosis disease (ahpnd) has recently emerged as a serious disease of cultured shrimp. it has also been described as early mortality syndrome (ems) due to mass mortalities occurring within 20 to 30 d after stocking of ponds with postlarvae. here, penaeus vannamei and penaeus monodon from shrimp farms in the philippines were examined for the toxin-producing strain of vibrio parahaemolyticus due to ahpnd-like symptoms occurring in marketable size shrimp. in the p. vannamei, ... | 2015 | 26503780 |
| changes in global gene expression of vibrio parahaemolyticus induced by cold- and heat-stress. | vibrio (v.) parahaemolyticus causes seafood-borne gastro-intestinal bacterial infections in humans worldwide. it is widely found in marine environments and is isolated frequently from seawater, estuarine waters, sediments and raw or insufficiently cooked seafood. throughout the food chain, v. parahaemolyticus encounters different temperature conditions that might alter metabolism and pathogenicity of the bacterium. in this study, we performed gene expression profiling of v. parahaemolyticus rimd ... | 2015 | 26498286 |
| [molecular detection methods for vibrio parahaemolyticus in seafood]. | to detect vibrio parahaemolyticus in seafood, we evaluated efficient combinations of molecular methods with dna extraction methods using heat extraction and alkaline heat extraction, and pcr, real-time pcr and loop-mediated isothermal amplification (lamp) assays were performed targeting v parahaemolyticus species-specific genes (tlh and rpod) and pathogenic factors genes (tdh and trh). the species-specific genes were detected in all combinations of two strains (a tdh * trh1-positive strain and a ... | 2015 | 26554219 |
| pathogenesis of acute hepatopancreatic necrosis disease (ahpnd) in shrimp. | acute hepatopancreatic necrosis disease (ahpnd), also called early mortality syndrome (ems), is a recently emergent shrimp bacterial disease that has resulted in substantial economic losses since 2009. ahpnd is known to be caused by strains of vibrio parahaemolyticus that contain a unique virulence plasmid, but the pathology of the disease is still unclear. in this study, we show that ahpnd-causing strains of v. parahaemolyticus secrete the plasmid-encoded binary toxin pirab(vp) into the culture ... | 2015 | 26549178 |
| cathepsin l is an immune-related protein in pacific abalone (haliotis discus hannai)--purification and characterization. | cathepsin l, an immune-related protein, was purified from the hepatopancreas of pacific abalone (haliotis discus hannai) by ammonium sulfate precipitation and column chromatographies of sp-sepharose and sephacryl s-200 hr. purified cathepsin l appeared as two bands with molecular masses of 28.0 and 28.5 kda (namely cathepsin la and lb) on sds-page under reducing conditions, suggesting that it is a glycoprotein. peptide mass fingerprinting (pmf) analysis revealed that peptide fragments of 95 amin ... | 2015 | 26549175 |
| transcriptional profiling of vibrio parahaemolyticus exsa reveals a complex activation network for type iii secretion. | vibrio parahaemolyticus (vp) is a marine halophilic bacterium that is commonly associated with oysters and shrimp. human consumption of contaminated shellfish can result in vp mediated gastroenteritis and severe diarrheal disease. vp encodes two type 3 secretion systems (t3ss-1 and t3ss2) that have been functionally implicated in cytotoxicity and enterotoxicity respectively. in this study, we profiled protein secretion and temporal promoter activities associated with exsa and exsb gene expressio ... | 2015 | 26539165 |
| microbiological quality of seafood marketed in taiwan. | seafood is often associated with foodborne illnesses, and vibrio parahaemolyticus is the most common pathogen implicated in outbreaks in taiwan. in this study, the microbiological quality of 300 raw or mixed ready-to-eat (rte) and other cooking-needed seafood samples was examined. the total aerobic and coliform counts of the rte samples were significantly higher than those of other cooking-needed samples. on average, 55.8 and 29.7% of the rte samples failed to meet the local microbiological stan ... | 2015 | 26555520 |
| [etiological surveillance for diarrhea in shanghai from august 2013 to july 2014]. | to understand the pathogen spectrum and epidemiological characteristics of diarrhea cases in shanghai from august 2013 to july 2014. | 2015 | 26837353 |
| the role of oncoprotein nm23 gene from exopalaemon carinicauda is response to pathogens challenge and ammonia-n stress. | oncoprotein nm23, as a family of genes encoding the nucleoside diphosphate (ndp) kinase, plays important roles in bioenergetics, dna replication, differentiation and tumor metastasis. in this study, a full-length cdna of nm23 (designated ecnm23) was cloned from exopalaemon carinicauda by using rapid amplification of cdna ends (race) approaches. the full-length cdna of ecnm23 was 755 bp, which contains an open reading frame (orf) of 518 bp, encoding a 175 amino-acid polypeptide with the predicted ... | 2015 | 26314522 |
| effect of dietary formic acid and astaxanthin on the survival and growth of pacific white shrimp (litopenaeus vannamei) and their resistance to vibrio parahaemolyticus. | a 90-day feeding trial was conducted to evaluate the effects of formic acid (fa) and astaxanthin (ax) on growth, survival, immune parameters, and tolerance to vibrio infection in pacific white shrimp. the study was divided into two experiments. in experiment 1, postlarvae-12 were randomly distributed into six groups and then fed four times daily with six experimental diets contained 0.3 % fa, 0.6 % fa, 50 ppm ax, 0.3 % fa + 50 ppm ax, 0.6 % fa + 50 ppm ax, or none of these supplements (control d ... | 2015 | 26312205 |
| epidemiological evidence of lesser role of thermostable direct hemolysin (tdh)-related hemolysin (trh) than tdh on vibrio parahaemolyticus pathogenicity. | vibrio parahaemolyticus carrying the tdh gene, encoding the thermostable direct hemolysin (tdh), or the trh gene, encoding the tdh-related hemolysin (trh), are both considered virulent strains. there are, however, disproportionally fewer reports of infections caused by seafood contaminated with trh-positive strains than by seafood contaminated with tdh-positive strains. bivalves such as clams and oysters are the major seafood varieties associated with the infections. in this study, the prevalenc ... | 2015 | 25646967 |
| draft genome sequences of four vibrio parahaemolyticus isolates from clinical cases in canada. | vibrio parahaemolyticus is a leading cause of bacterial gastroenteritis following ingestion of contaminated seafood. this report presents the draft genome sequences of four clinical strains of v. parahaemolyticus isolated in canada. all four strains lack traditional pathogenic markers and possess uniquely individual characteristics identified using other typing criteria. | 2015 | 25635013 |
| prevalence and antimicrobial susceptibility of vibrio parahaemolyticus isolated from retail shrimps in malaysia. | vibrio parahaemolyticus is a marine and estuarine bacterium that has been the leading cause of foodborne outbreaks which leads to a significant threat to human health worldwide. consumption of seafood contaminated with v. parahaemolyticus causes acute gastroenteritis in individuals. the bacterium poses two main virulence factor including the thermostable direct hemolysin (tdh) which is a pore-forming protein that contributes to the invasiveness of the bacterium in humans and tdh-related hemolysi ... | 2015 | 25688239 |
| characterization and expression analysis of toll-like receptor 2 gene in large yellow croaker, larimichthys crocea. | toll-like receptor 2 (tlr2) plays an important role in innate immune responses. here we describe the isolation and characterization of the full-length cdna sequence of toll-like receptor 2 in large yellow croaker larimichthys crocea (lctlr2). the lctlr2 cdna contains a 5'-terminal untranslated region (5'-utr) of 135 bp, an open reading frame (orf) of 2478 bp encoding a polypeptide of 825 amino acid residues and a 3'-utr of 50 bp. subcellular localization analysis suggested that the lctlr2-pegfp ... | 2015 | 25687392 |
| transoceanic spreading of pathogenic strains of vibrio parahaemolyticus with distinctive genetic signatures in the reca gene. | vibrio parahaemolyticus is an important human pathogen whose transmission is associated with the consumption of contaminated seafood. consistent multilocus sequence typing for v. parahaemolyticus has shown difficulties in the amplification of the reca gene by pcr associated with a lack of amplification or a larger pcr product than expected. in one strain (090-96, peru, 1996), the produced pcr product was determined to be composed of two reca fragments derived from different vibrio species. to be ... | 2015 | 25679989 |
| phenotypic and phylogenetic analysis of vibrio parahaemolyticus isolates recovered from diarrhea cases in guangdong province, china. | vibrio parahaemolyticus has emerged as a common foodborne pathogen of global concern. in this study, 108 v. parahaemolyticus isolates that recovered from diarrhea cases (n=96) and seafood products (n=12) in guangdong province from 2007 to 2011 were characterized by serotyping, tdh and trh toxin gene detection and multilocus sequence typing (mlst). the dominant serotypes from the cases were o3:k6, o4:k8 and o1: kut (untyped). however, most isolates recovered from seafood products belonged to othe ... | 2015 | 25662708 |
| vgrg2 of type vi secretion system 2 of vibrio parahaemolyticus induces autophagy in macrophages. | type vi secretion system (t6ss) is a macromolecular transenvelope machine encoded within the genomes of several proteobacteria species. vibrio parahaemolyticus contains two putative t6ss systems, vpt6ss1 and vpt6ss2, both contributing to adherence to caco-2 and/or hela cells. however, it remains unknown if these systems are involved in cellular responses. in order to exclude the effects of other virulence factors known to induce cytotoxicity or autophagy, a triple deletion mutant dttt (with dele ... | 2015 | 25784905 |
| characterization of vibrio parahaemolyticus clinical strains from maryland (2012-2013) and comparisons to a locally and globally diverse v. parahaemolyticus strains by whole-genome sequence analysis. | vibrio parahaemolyticus is the leading cause of foodborne illnesses in the us associated with the consumption of raw shellfish. previous population studies of v. parahaemolyticus have used multi-locus sequence typing (mlst) or pulsed field gel electrophoresis (pfge). whole genome sequencing (wgs) provides a much higher level of resolution, but has been used to characterize only a few united states (us) clinical isolates. here we report the wgs characterization of 34 genomes of v. parahaemolyticu ... | 2015 | 25745421 |
| development and evaluation of a loop-mediated isothermal amplification assay combined with enrichment culture for rapid detection of very low numbers of vibrio parahaemolyticus in seafood samples. | the aim of this study was to develop and evaluate a rapid and effective method to detect vibrio parahaemolyticus, a leading pathogen causing seafood-borne gastroenteritis. a newly designed loop-mediated isothermal amplification (lamp) assay including a short enrichment period was optimized. this assay correctly detected all the target strains (n=61) but none of the non-target strains (n=34). very low numbers of v. parahaemolyticus (2 colony forming unit (cfu) per gram of seafood) could be detect ... | 2015 | 25744462 |
| interaction between the type iii effector vopo and gef-h1 activates the rhoa-rock pathway. | vibrio parahaemolyticus is an important pathogen that causes food-borne gastroenteritis in humans. the type iii secretion system encoded on chromosome 2 (t3ss2) plays a critical role in the enterotoxic activity of v. parahaemolyticus. previous studies have demonstrated that t3ss2 induces actin stress fibers in various epithelial cell lines during infection. this stress fiber formation is strongly related to pathogenicity, but the mechanisms that underlie t3ss2-dependent actin stress fiber format ... | 2015 | 25738744 |
| molecular characterization of lvav in response to white spot syndrome virus infection in the pacific white shrimp (litopenaeus vannamei). | litopenaeus vannamei is the most important farmed shrimp species globally, but its production is affected by several factors, including infectious disease. white spot syndrome virus (wssv), in particular, causes significant shrimp losses. to understand the shrimp's immune response against wssv, we cloned lvav from l. vannamei and analyzed its expression pattern in different tissues, in addition to its expression following infection. we employed dsrna and recombinant (r)lvav to explore the potent ... | 2015 | 25735872 |
| intracellular survival of virulence and low-virulence strains of vibrio parahaemolyticus in epinephelus awoara macrophages and peripheral leukocytes. | in this study, we examined the virulence factors and pathogenesis of vibrio parahaemolyticus in epinephelus awoara. the chemotactic motility of v. parahaemolyticus for phagocytosis and intracellular survival in fish macrophages was determined using virulence strains and low-virulence strains of v. parahaemolyticus. we found that the intracellular mean number of virulence strains of v. parahaemolyticus ranged from 0-180 min after co-incubation with macrophages and peripheral leukocytes, was relat ... | 2015 | 25730008 |
| [molecular genetic makers for vibrio parahaemolyticus--a review]. | vibrio parahaemolyticus is an important foodborne pathogen, of which the 03:k6 serotype caused many outbreaks in different countries since 1996. based on the 10 years data (1992-2001) from china, gastroenteritis caused by vibrio parahaemolyticus accounted for 31.1% of foodborne disease outbreaks that were resulted from microorganisms. most environmental strains of vibrio parahaemolyticus are non-pathogenic strains. however, clinical strains can producethermostable direct hemolysin (tdh), tdh-rel ... | 2015 | 25958678 |
| prevalence and antimicrobial resistance of vibrio parahaemolyticus isolated from raw shellfish in poland. | vibrio parahaemolyticus is a marine bacterium recognized as an important cause of gastroenteritis in humans consuming contaminated shellfish. in recent years, increasing resistance to ampicillin and aminoglycosides has been observed among v. parahaemolyticus isolates. however, the first-line antimicrobials such as tetracyclines and fluoroquinolones remained highly effective against these bacteria. the aim of this study was to evaluate the occurrence of v. parahaemolyticus in live bivalve mollusc ... | 2015 | 25951402 |
| rna-seq analysis of macrobrachium rosenbergii hepatopancreas in response to vibrio parahaemolyticus infection. | the malaysian giant freshwater prawn, macrobrachium rosenbergii, is an economically important crustacean worldwide. however, production of this prawn is facing a serious threat from vibriosis disease caused by vibrio species such as vibrio parahaemolyticus. unfortunately, the mechanisms involved in the immune response of this species to bacterial infection are not fully understood. we therefore used a high-throughput deep sequencing technology to investigate the transcriptome and comparative exp ... | 2015 | 25922623 |
| pathogenic vibrio parahaemolyticus isolated from biofouling on commercial vessels and harbor structures. | ballast water is a significant vector of microbial dissemination; however, biofouling on commercial vessel hulls has been poorly studied with regard to pathogenic bacteria transport. biofouling on three commercial vessels and seven port structures in ensenada, baja california, mexico, was examined by qpcr to identify and quantify vibrio parahaemolyticus, a worldwide recognized food-borne human pathogen. pathogenic variants (trh+, tdh+) of v. parahaemolyticus were detected in biofouling homogenat ... | 2015 | 25921866 |
| first detection of ampc β-lactamase bla(cmy-2) on a conjugative inca/c plasmid in a vibrio parahaemolyticus isolate of food origin. | vibrio parahaemolyticus is an important causative agent of gastroenteritis, with the consumption of contaminated seafood being the major transmission route. resistance to penicillin is common among v. parahaemolyticus strains, whereas cephalosporin resistance remains rare. in an attempt to assess the current prevalence and characteristics of antibiotic resistance of this pathogen in common food samples, a total of 54 (17% of the total samples) v. parahaemolyticus strains were isolated from 318 m ... | 2015 | 25918142 |
| daxx from pacific white shrimp litopenaeus vannamei is involved in activation of nf-κb pathway. | death domain-associated factor 6 (daxx) is a fas-binding protein that mediates the activation of jun amino-terminal kinase (jnk) pathway and fas-induced apoptosis. in this study, a crustacean daxx (lvdaxx) was firstly cloned and identified from pacific white shrimp litopenaeus vannamei. the lvdaxx cdna was 2644 bp in length with an open reading frame (orf) of 2217 bp. sequence analysis indicated that lvdaxx contained a single daxx domain and two nuclear localization signals (nlss) and shared a s ... | 2015 | 25917972 |
| improvement of the quantitation method for the tdh (+) vibrio parahaemolyticus in molluscan shellfish based on most-probable- number, immunomagnetic separation, and loop-mediated isothermal amplification. | vibrio parahaemolyticus is a marine microorganism that can cause seafood-borne gastroenteritis in humans. the infection can be spread and has become a pandemic through the international trade of contaminated seafood. strains carrying the tdh gene encoding the thermostable direct hemolysin (tdh) and/or the trh gene encoding the tdh-related hemolysin (trh) are considered to be pathogenic with the former gene being the most frequently found in clinical strains. however, their distribution frequency ... | 2015 | 25914681 |
| the c-fos and c-jun from litopenaeus vannamei play opposite roles in vibrio parahaemolyticus and white spot syndrome virus infection. | growing evidence indicates that activator protein-1 (ap-1) plays a major role in stimulating the transcription of immune effector molecules in cellular response to an incredible array of stimuli, including growth factors, cytokines, cellular stresses and bacterial and viral infection. here, we reported the isolation and characterization of a cdna from litopenaeus vannamei encoding the full-length c-fos protein (named as lvc-fos). the predicted amino acid sequences of lvc-fos contained a basic-le ... | 2015 | 25912357 |
| genetic characterization of clinical and environmental vibrio parahaemolyticus from the northeast usa reveals emerging resident and non-indigenous pathogen lineages. | gastric infections caused by the environmentally transmitted pathogen, vibrio parahaemolyticus, have increased over the last two decades, including in many parts of the united states (us). however, until recently, infections linked to shellfish from the cool northeastern us waters were rare. cases have risen in the northeast, consistent with changes in local v. parahaemolyticus populations toward greater abundance or a shift in constituent pathogens. we examined 94 clinical isolates from a perio ... | 2015 | 25904905 |
| correction: characterization of trh2 harbouring vibrio parahaemolyticus strains isolated in germany. | 2015 | 25901603 | |
| opar controls a network of downstream transcription factors in vibrio parahaemolyticus bb22op. | vibrio parahaemolyticus is an emerging world-wide human pathogen that is associated with food-borne gastroenteritis when raw or undercooked seafood is consumed. expression of virulence factors in this organism is modulated by the phenomenon known as quorum sensing, which permits differential gene regulation at low versus high cell density. the master regulator of quorum sensing in v. parahaemolyticus is opar. opar not only controls virulence factor gene expression, but also the colony and cellul ... | 2015 | 25901572 |
| genome diversification within a clonal population of pandemic vibrio parahaemolyticus seems to depend on the life circumstances of each individual bacteria. | new strains of vibrio parahaemolyticus that cause diarrhea in humans by seafood ingestion periodically emerge through continuous evolution in the ocean. influx and expansion in the southern chilean ocean of a highly clonal v. parahaemolyticus (serotype o3:k6) population from south east asia caused one of the largest seafood-related diarrhea outbreaks in the world. here, genomics analyses of isolates from this rapidly expanding clonal population offered an opportunity to observe the molecular evo ... | 2015 | 25880192 |
| [genetic characterization of vibrio parahaemolyticus o3: k6 serovariant isolated in shenzhen]. | to characterize the o3: k6 serovariant of vibrio parahaemolyticus on virulence gene and molecular typing, and analyze the genetic relationship between o3: k6 and o3: k6 serovariants. | 2015 | 25876490 |
| gene expression profiles of vibrio parahaemolyticus in viable but non-culturable state. | viable but non-culturable (vbnc) state is referred to as a dormant state of non-sporulating bacteria enhancing the survival in adverse environments. to our knowledge, only few studies have been conducted on whole genomic expression of vibrio parahaemolyticus vbnc state. since a degradation of nucleic acids in v. vulnificus non-culturable state has been detected, we hypothesize that gene regulation of vbnc cells is highly reduced, downregulation of gene expression is dominant and only metabolic f ... | 2015 | 25873464 |
| a pandemic vibrio parahaemolyticus o3:k6 clone causing most associated diarrhea cases in the pacific northwest coast of mexico. | between september and october of 2004, more than 1230 cases of gastroenteritis due to pandemic o3:k6 strains of vibrio parahaemolyticus (v. parahaemolyticus) were reported in the relatively small geographical area of southern sinaloa, a state located in northwest mexico. since then, v. parahaemolyticus-associated gastroenteritis cases have gradually increased in prevalence spreading from south to north. the present study conducted an epidemiological surveillance of v. parahaemolyticus strains in ... | 2015 | 25852677 |
| insights into the environmental reservoir of pathogenic vibrio parahaemolyticus using comparative genomics. | vibrio parahaemolyticus is an aquatic halophilic bacterium that occupies estuarine and coastal marine environments, and is a leading cause of seafood-borne food poisoning cases. to investigate the environmental reservoir and potential gene flow that occurs among v. parahaemolyticus isolates, the virulence-associated gene content and genome diversity of a collection of 133 v. parahaemolyticus isolates were analyzed. phylogenetic analysis of housekeeping genes, and pulsed-field gel electrophoresis ... | 2015 | 25852665 |
| draft whole-genome sequences of 14 vibrio parahaemolyticus clinical isolates with an ambiguous k serogroup. | vibrio parahaemolyticus is a bacterial pathogen responsible for mild to severe gastroenteritis, wound infections, and septicemia resulting from the ingestion or handling of raw or undercooked contaminated seafood. here, we report the draft whole-genome sequences and annotations of 14 canadian v. parahaemolyticus clinical isolates that were serologically identified as k group ii using polyvalent antisera but were not specifically k serogrouped using monovalent antisera. | 2015 | 25838480 |
| use of whole-genome phylogeny and comparisons for development of a multiplex pcr assay to identify sequence type 36 vibrio parahaemolyticus. | vibrio parahaemolyticus sequence type 36 (st36) strains that are native to the pacific ocean have recently caused multistate outbreaks of gastroenteritis linked to shellfish harvested from the atlantic ocean. whole-genome comparisons of 295 genomes of v. parahaemolyticus, including several traced to northeastern u.s. sources, were used to identify diagnostic loci, one putatively encoding an endonuclease (prp), and two others potentially conferring o-antigenic properties (cps and flp). the combin ... | 2015 | 25832299 |
| [collection of vibrio parahaemolyticus species members: phenotypic and genotypic characteristics]. | formation of vibrio parahaemolyticus collection according to modern methodical opportunities and understanding of causative agent biology. | 2015 | 25816506 |
| complete genome sequences of a clinical isolate and an environmental isolate of vibrio parahaemolyticus. | vibrio parahaemolyticus is the leading cause of seafood-borne infections in the united states. we report complete genome sequences for two v. parahaemolyticus strains isolated in 2007, cdc_k4557 and fda_r31 of clinical and oyster origin, respectively. these two sequences might assist in the investigation of differential virulence of this organism. | 2015 | 25814612 |
| effect of climate change on the concentration and associated risks of vibrio spp. in dutch recreational waters. | currently, the number of reported cases of recreational- water-related vibrio illness in the netherlands is low. however, a notable higher incidence of vibrio infections has been observed in warm summers. in the future, such warm summers are expected to occur more often, resulting in enhanced water temperatures favoring vibrio growth. quantitative information on the increase in concentration of vibrio spp. in recreational water under climate change scenarios is lacking. in this study, data on oc ... | 2015 | 25809307 |
| carb-17 family of β-lactamases mediates intrinsic resistance to penicillins in vibrio parahaemolyticus. | vibrio parahaemolyticus is commonly resistant to ampicillin, yet the mechanisms underlying this phenomenon are not clear. in this study, a novel class a carbenicillin-hydrolyzing β-lactamase (carb) family of β-lactamases, bla(carb-17), was identified and found to be responsible for the intrinsic penicillin resistance in v. parahaemolyticus. importantly, bla(carb-17)-like genes were present in all 293 v. parahaemolyticus genome sequences available in genbank and detectable in all 91 v. parahaemol ... | 2015 | 25801555 |
| characterization of trh2 harbouring vibrio parahaemolyticus strains isolated in germany. | vibrio parahaemolyticus is a recognized human enteropathogen. thermostable direct hemolysin (tdh) and tdh-related hemolysin (trh) as well as the type iii secretion system 2 (t3ss2) are considered as major virulence factors. as tdh positive strains are not detected in coastal waters of germany, we focused on the characterization of trh positive strains, which were isolated from mussels, seawater and patients in germany. | 2015 | 25799574 |
| the pathogenesis, detection, and prevention of vibrio parahaemolyticus. | vibrio parahaemolyticus, a gram-negative motile bacterium that inhabits marine and estuarine environments throughout the world, is a major food-borne pathogen that causes life-threatening diseases in humans after the consumption of raw or undercooked seafood. the global occurrence of v. parahaemolyticus accentuates the importance of investigating its virulence factors and their effects on the human host. this review describes the virulence factors of v. parahaemolyticus reported to date, includi ... | 2015 | 25798132 |
| itraq-based proteomic profiling of vibrio parahaemolyticus under various culture conditions. | vibrio parahaemolyticus is a common pathogen infecting humans and marine animals; this pathogen has become a major concern of marine food products and trade. in this study, v. parahaemolyticus isolated from sewage was exposed to different culture conditions and analyzed by isobaric tag for relative and absolute quantitation (itraq) based reversed-phase liquid chromatography-tandem mass spectrometry (lc-ms/ms) technique. our goal is to gain further insights into the proteomics of v. parahaemolyti ... | 2015 | 26225125 |
| effects of dry storage and resubmersion of oysters on total vibrio vulnificus and total and pathogenic (tdh+/trh+) vibrio parahaemolyticus levels. | vibrio vulnificus (vv) and vibrio parahaemolyticus (vp) are the two leading causes of bacterial illnesses associated with raw shellfish consumption. levels of these pathogens in oysters can increase during routine antifouling aquaculture practices involving dry storage in ambient air conditions. after storage, common practice is to resubmerge these stored oysters to reduce elevated vv and vp levels, but evidence proving the effectiveness of this practice is lacking. this study examined the chang ... | 2015 | 26219373 |
| a cross-priming amplification assay coupled with vertical flow visualization for detection of vibrio parahaemolyticus. | vibrio parahaemolyticus is a marine seafood-borne pathogen that causes gastrointestinal disorders in humans. in this study, we developed a cross-priming amplification (cpa) assay coupled with vertical flow (vf) visualization for rapid and sensitive detection of v. parahaemolyticus. this assay correctly detected all target strains (n = 13) and none of the non-target strains (n = 27). small concentrations of v. parahaemolyticus (1.8 cfu/ml for pure cultures and 18 cfu/g for reconstituted samples) ... | 2015 | 26212475 |
| characterization and expression analysis of bca2 gene in large yellow croaker, larimichthys crocea. | bca2, as an e3 ubiquitin ligase, is an important anti-virus immune factor in mammals. up to date, there are not any related reports on bca2 protein in fishes yet. in the present investigation, bca2 in large yellow croaker larimichthys crocea (named as lcbca2) was identified and characterized. the full-length cdna of lcbca2 was 1571 bp, including an orf of 888 bp encoding a polypeptide of 295 amino acids. the putative lcbca2 protein contained a ring-h2 motif at c terminal. the lcbca2 transcripts ... | 2015 | 26208753 |
| raw tropical oysters as vehicles for multidrug-resistant vibrio parahaemolyticus. | the following study aimed to determine the antimicrobial susceptibility profile of vibrio parahaemolyticus strains from fresh and frozen oysters crassostrea rhizophorae sold in fortaleza-brazil. an antibiogram was performed on 87 isolates using nine antibiotics: gentamicin (gen 10 µg), ampicillin (amp 10 µg), penicillin g (pen 10u), ciprofloxacin (cip 5 µg), chloramphenicol (chl 30 µg), nalidixic acid (nal 30 µg), tetracycline (tet 30 µg), vancomycin (van 30 µg) and erythromycin (ery 15 µg). all ... | 2015 | 26200957 |
| differential expression of catalases in vibrio parahaemolyticus under various stress conditions. | among antioxidant enzymes, catalases protect microorganisms by degrading hydrogen peroxide under oxidative stress. in this study, the activities of at least four vibrio parahaemolyticus catalases (kat1 to kat4) were differentially detected during different growth stages and under various stress conditions using zymographic analysis. our results showed that only kat2 is stable at 55 °c. kat1 and kat2 respond to hydrogen peroxide during the early stationary and exponential growth phases, respectiv ... | 2015 | 26192211 |
| a highly sensitive and flexible magnetic nanoprobe labeled immunochromatographic assay platform for pathogen vibrio parahaemolyticus. | a magnetic nanoprobe labeled immunochromatographic test strip (mnp/icts) was developed to detect food-borne pathogen vibrio parahaemolyticus. specific antibody against v. parahaemolyticus was used as test line by coating onto the nitrocellulose membrane. magnetic nanoprobe was prepared by immobilizing the specific antibody onto the surface of superparamagnetic nanoparticles. specificity and sensitivity of the mnp/icts system were verified by artificially contaminated shrimp homogenate samples. r ... | 2015 | 26188497 |
| dna-binding protein hu coordinates pathogenicity in vibrio parahaemolyticus. | hu is one of the most abundant nucleoid-associated proteins in bacterial cells and regulates the expression of many genes involved in growth, motility, metabolism, and virulence. it is known that vibrio parahaemolyticus pathogenicity is related to its characteristic rapid growth and that type iii secretion system 1 (t3ss1) contributes to its cytotoxicity. however, it is not known if hu plays a role in the pathogenicity of v. parahaemolyticus. in the present study, we investigated the effect of h ... | 2015 | 26148713 |
| the structure of a gfp-based antibody (fluorobody) to tlh, a toxin from vibrio parahaemolyticus. | a fluorobody is a manmade hybrid molecule that is composed of green fluorescent protein (gfp) and a fragment of antibody, which combines the affinity and specificity of an antibody with the visibility of a gfp. it is able to provide a real-time indication of binding while avoiding the use of tags and secondary binding reagents. here, the expression, purification and crystal structure of a recombinant fluorobody for tlh (thermolabile haemolysin), a toxin from the lethal food-borne disease bacteri ... | 2015 | 26144238 |
| oxidative stress response of the black tiger shrimp penaeus monodon to vibrio parahaemolyticus challenge. | vibrio parahaemolyticus is a virulent pathogen that affects shrimp aquaculture. reactive oxygen species are produced by the immune system that defends the host against foreign microorganisms. in the present study, the oxidative stress response in hepatopancreas and gills of penaeus monodon to v. parahaemolyticus challenge were studied, such as respiratory burst, ros production (·o2(-) and ·oh), activities of antioxidant enzymes (cat, gpx, sod, pod and gst) and oxidative damage to lipid and prote ... | 2015 | 26142143 |
| draft genome sequence of vibrio parahaemolyticus vh3, isolated from an aquaculture environment in greece. | vibrio parahaemolyticus is an important foodborne pathogen responsible for gastroenteritis outbreaks globally. it has also been identified as an important pathogen in aquatic organisms. here, we report a draft genome sequence of v. parahaemolyticus, strain vh3, isolated from farmed juvenile greater amberjack, seriola dumerili, in greece. | 2015 | 26139725 |
| [analysis of antimicrobial resistance results of vibrio parahaemolyticus isolated from shellfish and its habitat in 2013]. | to evaluate the antimicrobial resistance of vibrio parahaemolyticus isolated from shellfish and its habitat in sichuan, fujian and guangxi. | 2015 | 26137614 |
| gene expression profiles of vibrio parahaemolyticus in the early stationary phase. | vibrio (v.) parahaemolyticus is an aquatic bacterium capable of causing foodborne gastroenteritis. in the environment or the food chain, v. parahaemolyticus cells are usually forced into the stationary phase, the common phase for bacterial survival in the environment. so far, little is known about whole genomic expression of v. parahaemolyticus in the early stationary phase compared with the exponential growth phase. we performed whole transcriptomic profiling of v. parahaemolyticus cells in bot ... | 2015 | 26118852 |
| examination of clinical and environmental vibrio parahaemolyticus isolates by multi-locus sequence typing (mlst) and multiple-locus variable-number tandem-repeat analysis (mlva). | vibrio parahaemolyticus is a leading cause of seafood-borne infections in the us. this organism has a high genetic diversity that complicates identification of strain relatedness and epidemiological investigations. however, sequence-based analysis methods are promising tools for these identifications. in this study, multi-locus sequence typing (mlst) and multiple-locus variable-number tandem-repeat analysis (mlva) was performed on 58 v. parahaemolyticus isolates (28 of oyster and 30 of clinical ... | 2015 | 26113844 |
| identification and functional characterization of the tab2 gene from litopenaeus vannamei. | in drosophila, tab2, an important intermediate in the imd signaling pathway, plays critical roles in the innate immune response in response to bacterial and viral infection. however, the role of tab-related proteins in the immune response of shrimp has not yet been established. here, we reported the identification of a tab2-like gene in litopenaeus vannamei designated as lvtab2. the full-length cdna of lvtab2 was 2160 bp with an open reading frame of 1827 bp, which encoded a putative protein of ... | 2015 | 26102459 |
| development of a rapid and simple immunochromatographic assay to identify vibrio parahaemolyticus. | to rapidly and simply determine whether or not bacterial colonies growing on agar were vibrio parahaemolyticus, we developed an immunochromatographic assay (vp-ica) using two different monoclonal antibodies (designated mab-vp34 and mab-vp109) against the delta subunit of v. parahaemolyticus-f0f1 atp synthase. the epitopes recognized by mab-vp34 and mab-vp109 were mapped to sequences of eight ((47)lltssfsa(54)) and six amino acid residues ((16)fdfavd(21)), respectively. an amino acid sequence sim ... | 2015 | 26093260 |
| temporal and spatial variation in the abundance of total and pathogenic vibrio parahaemolyticus in shellfish in china. | we investigated the abundance of total and pathogenic vibrio parahaemolyticus in shellfish sampled from four provinces in china during may 2013 and march 2014 using the most probable number-polymerase chain reaction (mpn-pcr) method. total v. parahaemolyticus was detected in 67.7% of 496 samples. a total of 38.1% and 10.1% of samples exceeded 1,000 mpn g(-1) and 10,000 mpn g(-1), respectively. v. parahaemolyticus densities followed a seasonal and geographical trend, with guangxi and sichuan shel ... | 2015 | 26061712 |
| corrigendum: the pathogenesis, detection, and prevention of vibrio parahaemolyticus. | [this corrects the article on p. 144 in vol. 6, pmid: 25798132.]. | 2015 | 26052313 |
| a repeat unit of vibrio diarrheal t3s effector subverts cytoskeletal actin homeostasis via binding to interstrand region of actin filaments. | a novel bacterial type iii secretion effector, vopv, from the enteric pathogen vibrio parahaemolyticus has been identified as a key factor in pathogenicity due to its interaction with cytoskeletal actin. one of the repeat units in the long repetitive region of vopv, named vopv(rep1), functions as an actin-binding module. despite its importance in pathogenesis, the manner in which the effector binds to actin and the subsequent effects on actin dynamics remain unclear. here, we report the molecula ... | 2015 | 26039684 |
| suspension of oysters reduces the populations of vibrio parahaemolyticus and vibrio vulnificus. | vibrio parahaemolyticus (vp) and vibrio vulnificus (vv) are associated with the consumption of raw oysters and cause illnesses ranging from simple gastroenteritis to life-threatening septicaemia. these halophilic bacteria are frequently found in marine and estuarine systems, accumulating within the tissues of a number of aquatic organisms and passing on to humans after consumption, through contaminated water, or via open wounds. as benthic organisms capable of filtering 40 gallons of water per h ... | 2015 | 26031606 |
| draft genome sequence of strain atcc 17802(t), the type strain of vibrio parahaemolyticus. | we report the draft genome of vibrio parahaemolyticus atcc 17802(t), containing 5067729 bp. the g+c content of the genome is 45.24 %. this strain possesses genes encoding a type iii secretion system 1, a type iii secretion system 2 and a tdh related hemolysin (trh). its taxonomically important phenotypes were also experimentally characterized. | 2015 | 26021549 |
| environmental influences on the seasonal distribution of vibrio parahaemolyticus in the pacific northwest of the usa. | populations of vibrio parahaemolyticus in the environment can be influenced by numerous factors. we assessed the correlation of total (tl+) and potentially virulent (tdh+) v. parahaemolyticus in water with three harmful algal bloom (hab) genera (pseudo-nitzschia, alexandrium and dinophysis), the abundance of diatoms and dinoflagellates, chlorophyll-a and temperature, salinity and macronutrients at five sites in washington state from 2008-2009. the variability in v. parahaemolyticus density was e ... | 2015 | 26454066 |
| evaluation of an immunochromatographic assay for direct identification of thermostable direct hemolysin-producing vibrio parahaemolyticus colonies on selective agar plates. | we evaluated the utility of an immunochromatographic assay (nh ic tdh) in identifying thermostable direct hemolysin (tdh)-producing vibrio parahaemolyticus colonies on selective agar plates. the sensitivity of the nh ic tdh assay was 100% (189 samples) and its specificity was 100% (41 samples) compared with the presence of tdh. | 2015 | 26432103 |
| prevalence and population analysis of vibrio parahaemolyticus in aquatic products from south china markets. | vibrio parahaemolyticus is a common foodborne pathogen in aquatic products. to investigate the prevalence of vibrio parahaemolyticus in aquatic products in south china, 224 samples were collected from markets in four provinces (11 cities) from may 2013 to january 2014. one hundred and fifty isolates were isolated from 98 samples. all isolates were analyzed for the presence of thermostable direct haemolysin (tdh) and tdh-related haemolysin (trh) by pcr, antibiotic susceptibility analysis by disk ... | 2015 | 26424767 |
| the small rna spot 42 regulates the expression of the type iii secretion system 1 (t3ss1) chaperone protein vp1682 in vibrio parahaemolyticus. | the cytotoxicity of vibrio parahaemolyticus has been related to the type iii secretion system 1 effector protein vp1680, which is secreted and translocated into host cells with the help of the specific chaperone protein, vp1682. this study sought to confirm the in silico analysis, which predicted that a small regulatory rna (spot 42) could base pair with the region encompassing the ribosomal-binding site and initiation codon of the vp1682 mrna. electrophoresis mobility shift assays indicated tha ... | 2015 | 26394644 |
| a foodborne outbreak of gastroenteritis caused by vibrio parahaemolyticus and norovirus through non-seafood vehicle. | foodborne outbreaks caused by a mixed infection of vibrio parahaemolyticus and norovirus have rarely been described. we reported a mixed outbreak of vibrio parahaemolyticus and norovirus causing acute gastroenteritis in 99 staff members of a company in guangdong, china, in may 2013, following consumption of roasted duck, an uncommon non-seafood vehicle for such mixed infection, in one meal served in the company's catering service. epidemiological and laboratory findings indicated that a single a ... | 2015 | 26376317 |
| multicolor melting curve analysis-based multilocus melt typing of vibrio parahaemolyticus. | vibrio parahaemolyticus is the leading cause of seafood-borne gastroenteritis outbreaks. to track the source of these diseases in a timely manner, a high throughput typing method is critical. we hereby describe a novel genotyping method for v. parahaemolyticus, termed multilocus melt typing (mlmt), based on multilocus sequence typing (mlst). mlmt utilizes melting curve analysis to interrogate the allelic types of a set of informative single nucleotide polymorphisms (snps) derived from the housek ... | 2015 | 26368129 |
| characterization and expression analysis of the prophenoloxidase activating factor from the mud crab scylla paramamosain. | prophenoloxidase activating factors (ppafs) are a group of clip domain serine proteinases that can convert prophenoloxidase (pro-po) to the active form of phenoloxidase (po), causing melanization of pathogens. here, two full-length ppaf cdnas from scylla paramamosain (spppaf1 and spppaf2) were cloned and characterized. the full-length spppaf1 cdna was 1677 bp in length, including a 5'-untranslated region (utr) of 52 bp, an open reading frame (orf) of 1131 bp coding for a polypeptide of 376 amino ... | 2015 | 26345816 |
| correction for lee et al., the opportunistic marine pathogen vibrio parahaemolyticus becomes virulent by acquiring a plasmid that expresses a deadly toxin. | 2015 | 26340988 | |
| expression profiles and interaction suggest tbk1 can be regulated by nrdp1 in response to immune stimulation in large yellow croaker larimichthys crocea. | tbk1 has been extensively studied in mammals because of its important roles as a molecular bridge, linking the tlrs (tlr3 and tlr4) and rlrs signals to activate transcriptional factors irf3 and irf7 for ifn-i production. however, the information on molecular and functional characteristics of tbk1 in teleosts is limited. in this study, the molecular characterization and immune response of tbk1 in larimichthys crocea (named as lctbk1) as well as its interaction with nrdp1 were investigated. sequen ... | 2015 | 26291490 |
| genotyping of virulence plasmid from vibrio parahaemolyticus isolates causing acute hepatopancreatic necrosis disease in shrimp. | acute hepatopancreatic necrosis disease (ahpnd) has caused severe mortalities in farmed penaeid shrimp throughout se asia and mexico. the causative agent of ahpnd is the marine bacterium vibrio parahaemolyticus, which secretes pira- and pirb-like binary toxin that caused deterioration in the hepatopancreas of infected shrimp. the genes responsible for the production of this toxin are located in a large plasmid residing within the bacterial cells. we analyzed the plasmid sequence from the whole g ... | 2015 | 26290509 |
| risk factors for vibrio parahaemolyticus infection in a southern coastal region of china. | the objectives of the study were to identify dietary and medical risk factors for vibrio parahaemolyticus (vp) infection in the coastal city shenzhen in china. | 2015 | 26287765 |
| assessing the function of stas domain protein sypa in vibrio fischeri using a comparative analysis. | colonization of the squid euprymna scolopes by vibrio fischeri requires biofilm formation dependent on the 18-gene symbiosis polysaccharide locus, syp. one key regulator, sypa, controls biofilm formation by an as-yet unknown mechanism; however, it is known that sypa itself is regulated by sype. biofilm-proficient strains form wrinkled colonies on solid media, while sypa mutants form biofilm-defective smooth colonies. to begin to understand the function of sypa, we used comparative analyses and m ... | 2015 | 26284045 |
| prevalences of pathogenic and non-pathogenic vibrio parahaemolyticus in mollusks from the spanish mediterranean coast. | vibrio parahaemolyticus is a well-recognized pathogen of humans. to better understand the ecology of the human-pathogenic variants of this bacterium in the environment, a study on the prevalence in bivalves of pathogenic variants (tlh+ and tdh+ and/or trh+) versus a non-pathogenic one (only tlh+ as species marker for v. parahaemolyticus), was performed in two bays in catalonia, spain. environmental factors that might affect dynamics of both variants of v. parahaemolyticus were taken into account ... | 2015 | 26284033 |
| [the infection and molecular characteristics of vibrio parahaemolyticus isolated from intestinal outpatient in two sentinel hospitals in shanghai, 2010-2012]. | to analyze virulence genes and molecular characteristics of vibrio parahaemolyticus isolated from sporadic cases with diarrhea in tow sentinel hospitals of shanghai, 2010-2012. | 2015 | 26268867 |
| binding interaction of sglt with sugar and thiosugar by the molecular dynamics simulation. | the human sodium-glucose co-transporter 2 (hsglt2) is a transporter responsible for reabsorption of glucose in the proximal convoluted tubule of the kidney. hsglt2 inhibitors, including luseogliflozin, have been developed as drugs for type 2 diabetes mellitus. only luseogliflozin contains a thiosugar ring in its chemical structure, while other hsglt2 inhibitors contain glucose rings. consequently, we focused on the binding interactions of hsglt2 with sugars and thiosugars. we first revealed that ... | 2015 | 26260238 |
| strain-level visualized analysis of cold-stressed vibrio parahaemolyticus based on maldi-tof mass fingerprinting. | in this study, strain-level visualized analysis of cold-stressed vibrio parahaemolyticus based on matrix-assisted laser desorption ionization time-of-flight (maldi-tof) mass fingerprinting was investigated. all the peptide mass fingerprinting profiles obtained were analyzed by self-organized map (som) and cluster analysis. our results showed that the peptide mass fingerprinting profiles of v. parahaemolyticus substantially changed under cold stress at strain level. the cold-stressed v. parahaemo ... | 2015 | 26255031 |
| long-term study of vibrio parahaemolyticus prevalence and distribution in new zealand shellfish. | the food-borne pathogen vibrio parahaemolyticus has been reported as being present in new zealand (nz) seawaters, but there have been no reported outbreaks of food-borne infection from commercially grown nz seafood. our study determined the current incidence of v. parahaemolyticus in nz oysters and greenshell mussels and the prevalence of v. parahaemolyticus tdh and trh strains. pacific (235) and dredge (21) oyster samples and mussel samples (55) were obtained from commercial shellfish-growing a ... | 2015 | 25616790 |
| epidemic clones, oceanic gene pools, and eco-ld in the free living marine pathogen vibrio parahaemolyticus. | we investigated global patterns of variation in 157 whole-genome sequences of vibrio parahaemolyticus, a free-living and seafood associated marine bacterium. pandemic clones, responsible for recent outbreaks of gastroenteritis in humans, have spread globally. however, there are oceanic gene pools, one located in the oceans surrounding asia and another in the mexican gulf. frequent recombination means that most isolates have acquired the genetic profile of their current location. we investigated ... | 2015 | 25605790 |
| identification of a serum amyloid a gene and the association of snps with vibrio-resistance and growth traits in the clam meretrix meretrix. | serum amyloid a (saa), an acute response protein as well as an apolipoprotein, is considered to play crucial roles in both innate immunity and lipid metabolism. in this study, a saa gene (mmsaa) was identified in the clam meretrix meretrix. the full length dna of mmsaa was 1407bp, consisting of three exons and two introns. the distribution of mmsaa in clam tissues was examined with the highest expression in hepatopancreas. in response to the vibrio parahaemolyticus challenge, mmsaa mrna showed s ... | 2015 | 25602707 |
| complete genome sequence of vpkk5, a novel vibrio parahaemolyticus lytic siphophage. | this paper describes the complete sequence of a novel lytic marine siphophage, vpkk5, that is specific to vibrio parahemolyticus. | 2015 | 25573936 |
| a c-type lectin (lvctl4) from litopenaeus vannamei is a downstream molecule of the nf-κb signaling pathway and participates in antibacterial immune response. | c-type lectins (ctls) play multiple roles in innate immune defense against invading pathogens in both vertebrates and invertebrates. in this study, a new c-type lectin gene from pacific white shrimp litopenaeus vannamei (designated as lvctl4) was cloned by rapid amplification of the cdna ends (race) method. the full-length cdna of lvctl4 was 563 bp with open reading frame (orf) of 471 bp encoding a polypeptide of 156 amino acids, including a putative signal sequence and a single c-type lectin-li ... | 2015 | 25559446 |
| bacteriostatic suppression in norway lobster (nephrops norvegicus) exposed to manganese or hypoxia under pressure of ocean acidification. | future ocean acidification (oa) and warming following climate change elicit pervasive stressors to the inhabitants of the sea. previous experimental exposure to oa for 16 weeks at ph levels predicted for 2100 has shown to result in serious immune suppression of the norway lobster, nephrops norvegicus. the lobsters are currently affected by stressors such as periodical hypoxia inducing high levels of bioavailable manganese (mn). here, we aimed to investigate possible effects of interactions betwe ... | 2015 | 25553539 |