Publications

fatal infectious mononucleosis and virus-associated hemophagocytic syndrome.	a detailed clinicopathologic analysis of 52 cases of fatal infectious mononucleosis was performed. fever, rash, generalized lymphadenopathy, hepatosplenomegaly, and blood cytopenias were the characteristic findings. epstein-barr virus infection was documented in 44 of the 52 patients. a triphasic process evolved in the blood and bone marrow of 43 patients. early, the leukocyte count was elevated due to numerous atypical lymphoid cells, and the marrow was hyperplastic. later, severe pancytopenia ...	1987	3579509
storage conditions and stability of thyrotropin and thyroid hormones on filter paper.	we evaluated the effects of temperature and humidity on thyroid hormones (t4, t3) and thyrotropin (tsh) measured in blood spots dried on filter paper. rias for t4 and t3 blood spots were optimized to measure these analytes over the neonatal and euthyroid adult reference intervals. sensitivities of the t3 and t4 assays were 0.5 and 10 nmol/l, respectively. a blood-spot immunoradiometric assay for tsh involving magnetizable particles was developed with a sensitivity of 6 milli-int. units/l. contro ...	1987	3594835
storage conditions and stability of thyrotropin and thyroid hormones on filter paper.	we evaluated the effects of temperature and humidity on thyroid hormones (t4, t3) and thyrotropin (tsh) measured in blood spots dried on filter paper. rias for t4 and t3 blood spots were optimized to measure these analytes over the neonatal and euthyroid adult reference intervals. sensitivities of the t3 and t4 assays were 0.5 and 10 nmol/l, respectively. a blood-spot immunoradiometric assay for tsh involving magnetizable particles was developed with a sensitivity of 6 milli-int. units/l. contro ...	1987	3594835
changes in hemodynamics and myocardial contractility during chronic sodium depletion in conscious dogs.	chronic sodium depletion has been reported to decrease ejection fraction in anesthetized dogs. we tested the hypothesis that this reduction in cardiac performance is due to either hemodynamic or humoral factors. seven mongrel dogs were fed a low sodium diet (less than 2 meq na+ per day) for 5 weeks. echocardiographic and radionuclide techniques were used to monitor cardiac function. there was a gradual but significant (p less than 0.01) decrease in ejection fraction from 61 +/- 7% (sd) at baseli ...	1987	3596785
effect of organic vehicles on the pharmacokinetics of aminophylline administered intravenously to goats.	aminophylline dissolved in water, propylene glycol, or dimethyl sulfoxide was administered intravenously to goats in a randomized cross-over experiment. model-dependent and model-independent pharmacokinetic parameters for theophylline were compared on the basis of the solvent used in the dosage form administered. no difference was found in any pharmacokinetic parameter. thus, we found no evidence for the possibility that the organic solvents studied would confound pharmacokinetic investigations ...	1987	3612942
pentapeptide nuclear localization signal in adenovirus e1a.	the adenovirus e1a gene products are nuclear proteins important in transcriptional control of viral functions during infection. by producing normal e1a proteins and derivatives of e1a in bacteria and microinjecting these proteins into cultured cells, we were able to examine their ability to localize to the nucleus. we showed that a short peptide sequence at the carboxyl terminus of e1a is necessary for the rapid (30-min) nuclear localization of that protein. additionally, we showed that just the ...	1987	3614197
link protein interactions with hyaluronate and proteoglycans. characterization of two distinct domains in bovine cartilage link proteins.	hyaluronic acid-binding region and trypsin-link protein were prepared from bovine nasal cartilage proteoglycan complex after trypsin digestion. binary complexes were reformed between trypsin-link protein and hyaluronic acid-binding region or hyaluronate. upon trypsin treatment of these complexes, two fragments deriving from trypsin-link protein were characterized. one of them, of 20 kda, corresponds in fact to a 140-amino acid long fragment and bears the glycosylated site of trypsin-link protein ...	1987	3654611
liposome as an adjuvant for the production of estradiol antibodies.	mice immunized with liposome associated estradiol-bovine serum albumin conjugate (e2-bsa) showed a significantly higher estradiol-specific immune response than did mice injected with the free conjugate. the conjugation of estradiol with bsa is imperative, since injection of liposomes with estradiol in the lipid bilayer was found to be ineffective in inducing an immune response. entrapment of e2-bsa in neutral and positively charged liposomes was found to be a potent mode of immunostimulation, ev ...	1987	3666803
genetic comparison of lav-related isolates.	lav/htlv-iii has been found to be the etiological cause of aids. this new human retrovirus has a selective tropism for t lymphocytes of the okt4/leu 3 subset, in which it induces a cytopathic effect. we have compared southern blot patterns of integrated proviral dnas from different individuals at risk or not using a nick-translated lav probe. we find that lav/htlv-iii is very similar to our haitian isolate and close to an isolate from an early-recognized (in 1982) aids case in new york. more var ...	1987	2826003
genetic comparison of lav-related isolates.	lav/htlv-iii has been found to be the etiological cause of aids. this new human retrovirus has a selective tropism for t lymphocytes of the okt4/leu 3 subset, in which it induces a cytopathic effect. we have compared southern blot patterns of integrated proviral dnas from different individuals at risk or not using a nick-translated lav probe. we find that lav/htlv-iii is very similar to our haitian isolate and close to an isolate from an early-recognized (in 1982) aids case in new york. more var ...	1987	2826003
[antigenic determinants of bacteria. the structure of the repeating unit of a specific polysaccharide from shigella boydii type 7].	acid hydrolysis of the antigenic lipopolysaccharide from shigella boydii type 7 afforded a specific polysaccharide composed of 2-acetamido-2-deoxy-d-glucose, d-glucose, d-galactose, 5-acetamido-3,5,7,9-tetradeoxy-7-[(3r)-3-hydroxybutyramido]-l- glycero-l-manno-nonulosonic acid (nonn2a) and acetic acid residues in the 1:1:2:1:1 ratio. from the results of methylation analysis, hydrogen fluoride solvolysis and smith degradation, the structure of the repeating unit of the specific polysaccharide was ...	1987	2437931
influence of neuropeptides on airway smooth muscle.	the first bronchoactive (and vasoactive) peptide to be discovered in the lung was isolated and characterized in 1970 from an embryologically related organ, the small intestine. since then, more than 20 additional peptides have been described in lung tissue and their biologic activities investigated. many of these are neuropeptides acting as neurotransmitters or neuromodulators to influence airway, pulmonary vascular, and other functions. more neuropeptides are known to exist in the brain and per ...	1987	2446543
[antigenic polysaccharides of bacteria of the genus shigella. determination of the structure of polysaccharide chains of shigella boydii type 9 lipopolysaccharide and detection of unusually high molecular weight glycolipid].	specific acidic polysaccharide has been isolated from the shigella boydii type 9 antigenic lipopolysaccharide after mild hydrolysis followed by chromatography on sephadex g-50. the polysaccharide consists of d-glucose, d-glucuronic acid, 2-acetamido-2-deoxy-d-glucose, and l-rhamnose. from the results of methylation analysis, partial acid hydrolysis and 13c nmr data the structure of the repeating unit of the polysaccharide was deduced as follows: [----4)dglcp(alpha 1----4)dglcap(beta 1----3)dglcn ...	1987	2447896
[antigenic polysaccharides of bacteria of the genus shigella. the structure of the polysaccharide chain of shigella boydii type 14].	a specific acidic polysaccharide has been isolated from the shigella boydii type 14 antigenic lipopolysaccharide after mild hydrolysis followed by chromatography on sephadex g-50. the polysaccharide consists of the d-glucuronic acid, 2-acetamido-2-deoxy-d-glucose and d-galactose residues in the ratio 1:1:3. from the results of methylation analysis and partial acid hydrolysis, the structure of the repeating unit of the specific polysaccharide was deduced as follows: (-6dgalp alpha 1-4dglcap beta ...	1987	2447897
malignant cystosarcoma phyllodes associated with scirrhous carcinoma of the breast: a case report.	the presence of a malignant cystosarcoma phyllodes in one breast and a scirrhous carcinoma in the opposite breast in a premenopausal black woman is described. malignant cystosarcoma phyllodes is uncommon and its rare association with carcinoma of the breast is mentioned.	1987	204062
predicted conformations for the immunodominant region of the circumsporozoite protein of the human malaria parasite plasmodium falciparum.	the circumsporozoite protein of the human malaria parasite plasmodium falciparum contains multiple tandem repeats of the amino acid sequence asn-ala-asn-pro. the repeated sequence encompasses the immunodominant region of the protein, and antibodies raised against it are potent inhibitors of invasion and development of sporozoites in cultured hepatocytes. using a modified build-up procedure, we have explored a large number of possible helical and near-helical conformations of a terminally blocked ...	1986	2426702
[somatic antigens of shigella and escherichia coli. determination of the structure of o-specific polysaccharide from shigella boydii type 12 and its serological properties compared with the o-specific polysaccharide from escherichia coli].	the structure of the o-specific polysaccharide of the somatic antigen (lipopolysaccharide) of shigella boydii, type 12, was established by 1h- and 13c-nmr, methylation analysis and partial acid hydrolysis methods. the polysaccharide consists of pentasaccharide repeating units of the following structure: (formula; see text) the amount of o-acetyl groups was far less than stoichiometric, only about 2 for 3-4 repeating units. nevertheless, the results of serological studies revealed 3-o-acetyl-alph ...	1986	2430583
[intergeneric antigenic relation of hafnia and shigella (s. flexneri and s. boydii) bacteria].	the o-antigenic relationships between hafnia and shigellae (s. flexneri and s. boydii) have been studied. for the first time the presence of antigenic relationship between hafnia o19, o4, o9, o33, o5, o16, o12, o7, o29, o28, o10, o32, o24, o25, o18, o1, o13, o3, o22, o30, o37, o14, o11, o25, o23, o21, o28, o16, o24, o8, o26, o27 and s. flexneri la, lb, 2a, 2b, 4a, 4b, 6, 5a, 5b, as well as between hafnia o10, o21, o35, o36, o9, o28, o8, o30 and s. boydii 1, 3, 6, 14, 2, 5, 2, 12 have been reveal ...	1986	2431565
sporidesmin and gliotoxin induce cell detachment and perturb microfilament structure in cultured liver cells.	changes in cell morphology and cell adhesion occurred when cultured cells from the rat liver cell strain c3 were exposed to the fungal toxins, sporidesmin or gliotoxin. both toxins caused loss of attachment of the cells to the plastic of tissue culture plates and this effect was preceded by loss of actin cables. other changes included cytoplasmic vacuolation and blocked entry into s-phase of the cell cycle. under these conditions [3h]thymidine incorporation into the cells was also diminished but ...	1986	2432076
somatotopic organization of motoneurons innervating the pronators, carpal and digital flexors and forepaw muscles in the dog: a retrograde horseradish peroxidase study.	central distribution of motor neurons innervating the individual forearm and forepaw muscle through the median and ulnar nerves was studied in the dog using the retrograde horseradish peroxidase (hrp) method. all hrp-labeled cells were seen ipsilaterally in restricted parts within the dorsolateral (dl) and retrodorsolateral nucleus (rdl) of the ventral horn at levels from the cranial tip of c7 to the cranial third of t2. the results showed that motoneurons situated more cranially supply the more ...	1986	3708348
[fasciculation of the median nerve in adults and in the human fetus].		1986	3726653
establishment of passive hemagglutination assay (pha) system for anti-hbc in plasma.	a sensitive and specific assay system for anti-hbc, the passive hemagglutination (pha) method, has been established. the reactivity of pha cells prepared by conjugating purified recombinant hbcag-particles with fixed sheep blood cells (srbc) was highly specific to monoclonal- and polyclonal anti-hbc iggs. the sensitivity of pha method was higher than that of radioimmunoassay (ria). however, uncertainty for the positivity of anti-hbc still remained in plasma with the pha titers lower than 2(5). a ...	1986	3738912
1,25-dihydroxyvitamin d3 treatment results in increased choline acetyltransferase activity in specific brain nuclei.	to better understand the role of 1,25-dihydroxyvitamin d3 [1,25-(oh)2d3] in brain function, the level of calcium-binding protein (cabp) and the activities of choline acetyltransferase (cat) and monoamine oxidase were measured in discrete brain nuclei of vitamin d-deficient and -replete male rats. the nuclei sampled were those in which receptors for 1,25-(oh)2d3 and/or vitamin d-dependent cabp have been localized. significant elevations in cat activity were found in the arcuatemedian eminence and ...	1986	3753932
[establishment of a spontaneously transformed human fetal lung cell line and its biological characteristics].	a spontaneously transformed human fetal lung cell line (hflt) was derived from a human fetal lung diploid fibroblast cell line (2bs) by continuous culture. the biological characteristics of this cell line were studied and compared with those of 2bs cells. accompanying the morphological alteration, the growth rate of the transformed cell was accelerated and the maximum cell density was increased. the anchorage-independent growth was shown by its ability to form colonies in soft agar. the collagen ...	1986	3769747
laser-assisted vasal anastomosis in the rat and man.	in order to study the efficacy of laser-assisted vasal anastomosis (lava), a microscopic carbon dioxide surgical laser (xanar) was utilized in the anastomoses of human vas deferens in vitro and the sprague-dawley rat vas deferens in vivo. the longitudinal tensile and internal hydrostatic pressure strengths of laser-assisted vasal anastomoses were compared to conventional microsurgical suture anastomoses in the human vas deferens. the lava group had a greater mean internal hydrostatic pressure st ...	1986	3773082
[an attempt to create a factorial model of the epizootic process in sheep infected with s. abortus ovis].	experiments were carried out to build up a factorial model with a group of sheep at different age in an infected region. studied were the aspects of the disease as caused by salmonella abortus ovis alone and in combination with chl. ps. var. ovis. it was found that the factorial model can explain sufficiently well (77.22 per cent) the discussed system--the epizootic process with year-old females and sheep affected with pure and mixed infection. the epizootic process with a mixed infection in the ...	1986	3811206
excitatory amino acid transmitters in neuronal circuits of the cat visual cortex.	to test a possibility that glutamate (glu) and aspartate (asp) are transmitters in the visual cortex and to locate their operating sites in the cortical circuitry, we studied effects of microiontophoretic application of glu/asp antagonists on visual responses of cortical neurons in the cat. the antagonists tested were kynurenic acid (kyna), cis-2,3-piperidine dicarboxylic acid, and gamma-d-glutamylglycine. among these antagonists, kyna was most effective in blocking visual responses of cortical ...	1986	2870137
integrating treatments in schizophrenia.	schizophrenia is a common and severe illness found throughout the world. onset is usually in adolescence and young adulthood, and many individuals have illness-related impairments for life. schizophrenia assaults the very essence of what is distinctly human. the personality is devastated, subjective experience and thought distorted, and the psychological building blocks of everyday life torn asunder. this article discusses diagnostic and conceptual considerations, pharmacotherapy, and interperso ...	1986	2870478
a follow up of t-cell subsets and of anti-m. leprae antibody titer as measured by the fla-abs test in melanesian leprosy patients under polychemotherapy.	melanesian leprosy patients from new caledonia were studied for the following parameters during the course of polychemotherapy: peripheral blood t-cell subsets, as identified in an immunofluorescence assay with monoclonal antibodies okt3 ("pan-t"), okt4 ("helper/inducer"), and okt8 ("cytotoxic-suppressor"), and anti-mycobacterium leprae antibodies in the serum, as measured by the fluorescent leprosy antibody absorption test. a group of melanesian healthy subjects with no known exposure to m. lep ...	1986	2940306
dose-related effects of synthetic human beta-endorphin and naloxone on fed gastrointestinal motility.	in humans, plasma beta-endorphin levels rise during application of acute stressful stimuli (vertigo, cold pain, and transcutaneous electrical stimulation) that induce gut motor disturbances. whereas it is possible that circulating beta-endorphin participates in the mediation of these central effects on gut motility, its role cannot be established solely on the basis of changes in plasma levels. therefore, we designed the present study to investigate 1) the dose-related effects of intravenous syn ...	1986	2942039
structure-activity relationships of sex steroid analogs determined in vitro in a thyroid hormone-responsive membrane ca2+-atpase model.	physiological concentrations of l-t4 were found previously to stimulate ca2+-atpase activity in vitro in reticulocyte membranes from female rabbits and to inhibit this enzyme in reticulocyte membranes from males. in these previous studies, preincubation of intact cells or ghosts with testosterone (5 x 10(-11) m) converted female-source reticulocyte membranes to male-type responsiveness to thyroid hormone (inhibition of ca2+-atpase activity). preincubation of reticulocyte membranes with 17 beta-e ...	1986	2946570
structure-activity relationships of sex steroid analogs determined in vitro in a thyroid hormone-responsive membrane ca2+-atpase model.	physiological concentrations of l-t4 were found previously to stimulate ca2+-atpase activity in vitro in reticulocyte membranes from female rabbits and to inhibit this enzyme in reticulocyte membranes from males. in these previous studies, preincubation of intact cells or ghosts with testosterone (5 x 10(-11) m) converted female-source reticulocyte membranes to male-type responsiveness to thyroid hormone (inhibition of ca2+-atpase activity). preincubation of reticulocyte membranes with 17 beta-e ...	1986	2946570
occurrence of small hsd plasmids in salmonella typhi, shigella boydii, and escherichia coli.	the natural occurrence of small hsd (host specificity for dna) plasmids was demonstrated in restriction endonuclease-producing strains of salmonella typhi, shigella boydii, and escherichia coli. the five hsd plasmids isolated were between 5.0 and 12.2 kilobases long. the copy number of all the hsd plasmids was high (more than 10 copies per cell). introduction of these small plasmids into e. coli strain 0 drastically lowered the efficiency of plating of the lambda.0 phages (the efficiency of plat ...	1986	3003023
differential effects of transforming growth factor type beta on the growth and function of adrenocortical cells in vitro.	transforming growth factor type beta (tgf-beta) suppresses basal as well as corticotropin (acth)-stimulated steroid formation by bovine adrenocortical cells in culture. the effect is dose dependent and is not accompanied by any change in adrenocortical cell growth. the minimum effective dose of tgf-beta is 4 x 10(-13) m (10 pg/ml), and maximal inhibition is observed at a concentration of 4 x 10(-11) m (1 ng/ml). a 16- to 20-hr incubation with tgf-beta is required to decrease steroidogenesis, and ...	1986	3020557
differential effects of transforming growth factor type beta on the growth and function of adrenocortical cells in vitro.	transforming growth factor type beta (tgf-beta) suppresses basal as well as corticotropin (acth)-stimulated steroid formation by bovine adrenocortical cells in culture. the effect is dose dependent and is not accompanied by any change in adrenocortical cell growth. the minimum effective dose of tgf-beta is 4 x 10(-13) m (10 pg/ml), and maximal inhibition is observed at a concentration of 4 x 10(-11) m (1 ng/ml). a 16- to 20-hr incubation with tgf-beta is required to decrease steroidogenesis, and ...	1986	3020557
exposure of sheep to natural aerosols of foot-and-mouth disease virus.	sheep taken individually and allowed to inhale air being drawn along a duct from a cabinet containing pigs acutely infected with foot-and-mouth disease virus for 10 or 15 minute periods were infected by doses as low as 10 tcid50 of virus. the most consistent and reliable indicators of infection were viraemia and seroconversion. the mean times from exposure to onset of viraemia, pyrexia and the appearance of vesicular lesions were 2.5, 3.8 and 4.7 days, respectively. neither the time from exposur ...	1986	3020658
production of restriction endonucleases using multicopy hsd plasmids occurring naturally in pathogenic escherichia coli and shigella boydii.	a convenient procedure has been devised for detection of restriction endonucleases in the escherichia coli-shigella group. with this procedure, two restriction endonucleases, designated sbo 13 and eco t22, were found and later were identified as isoschizomers of nrui and avaiii, respectively. these endonucleases were shown to be produced from small multicopy plasmids. they were isolated from nonpathogenic e. coli into which the plasmids had been introduced by transformation, and purified from co ...	1986	3021586
relative activities of 5'-nucleotidase and adenosine deaminase in atrial and ventricular myocardium--the enzyme paradox.	the 5'-nucleotidase and adenosine deaminase activities were determined for tissue extracts of up to 10 defined regions of the normal dog heart. the specific activity of 5'-nucleotidase was very significantly higher in atrial than in ventricular myocardium (4 x) and was also higher in right than in left sites of the heart (2 x). the reason for the markedly higher specific activity in atrial muscle is not clear and appears paradoxical relative to adenine nucleotide content and pattern of atp decay ...	1986	3025011
[development of the classification of bacteria in the genus shigella].		1986	3518304
the relation between production of cytotoxin and clinical features in shigellosis.	the relation between in vitro production of hela cell cytotoxin by strains of shigella and clinical symptomatology was determined for 35 travelers from the united states who developed shigellosis in guadalajara, mexico. there were 25 patients with shigella sonnei, eight with shigella flexneri, one with shigella boydii, and one with shigella dysenteriae. these strains were evaluated for in vitro production of cytotoxin. the amount of cytotoxin did not correlate with the number of stools passed, t ...	1986	3519788
[conjugative r-plasmids simultaneously belonging to the 2 incompatibility groups inc i zeta and inc b].	five plasmids detected in the clinical strains of shigella were studied. the plasmids had similar phenotypes of antibiotic resistance and allotted streptomycin and tetracycline resistance to the host cell. none of them allotted the bacterial cells with sensitivity to phages specific for the inc groups f, n, p and i. the molecular weights of the plasmids ranged from 47 to 50 md. the studies on the incompatibility showed that pkmr 227, pkmr 228-1, pkmr 231, pkmr 242-1 and pkmr 245 plasmids simulta ...	1986	3521469
comparison of dna probes and the sereny test for identification of invasive shigella and escherichia coli strains.	forty-two shigella and 29 escherichia coli strains were screened for invasiveness in the sereny test and for hybridization with two recently described dna probes for the invasiveness plasmid. both probes produced identical results. all sereny-positive strains hybridized with both dna probes. three sereny-negative strains also hybridized with the probes, suggesting that there are strains containing the invasiveness plasmid that are not pathogenic in animal models.	1986	3531233
production of shiga toxin and other cytotoxins by serogroups of shigella.	we measured the cytotoxic activity of 119 strains of shigella by using a quantitative [3h]thymidine-labeled hela cell assay. we assayed 13 strains of shigella dysenteriae 1; 18 strains of s. dysenteriae types 2 and 3; and 88 strains of shigella sonnei, shigella flexneri, and shigella boydii. strains of s. dysenteriae 1 demonstrated high levels of cytotoxicity (geometric mean, 10(5.04) cd50/mg of protein; range, 10(3.95)-10(6.10). cytotoxic activities of the non-type 1 strains of s. dysenteriae a ...	1986	3537154
shigella from ovine faeces.		1986	3541356
isotope exchange at equilibrium studies with rat muscle adenylosuccinate synthetase.	the kinetic mechanism of rat muscle adenylosuccinate synthetase was studied by determining the rates of isotope exchange at equilibrium. a random sequential binding mechanism was indicated for both the forward and reverse reactions. aspartate, adenylosuccinate, gdp, and pi were determined to bind in rapid equilibrium. gtp exchanges with both gdp and pi at the same rate, which is similar to the exchange rate of imp with adenylosuccinate. aspartate exchanges with adenylosuccinate at a higher rate ...	1986	3542024
[bacteriological study of shigellosis in the lake kivu area (central africa). developments in the last 15 years (1968-1983)].	1913 shigella strains were isolated from 1968 to 1983 in lake kivu area (central africa). distribution and evolution of serotypes, biotypes and antibiotypes were studied. the majority of serotypes and biotypes were stable. however, few of these strains showed variations, among them shigella dysenteriae serotype 1, causing epidemic dysenteriae since 1981. epidemiologic survey of shigellosis in kivu seems to be rather underdeveloped (4 bacteriology laboratories for an area of 10 million inhabitant ...	1986	3542256
on dying more than one death.	death (with a capital d) can best be understood as a series of distinct but related deaths. for example, a pregnant woman was found to be brain-dead but her vital functions were artificially sustained for nine weeks until her fetus could be delivered, after which the machines were removed and she died a second, conceptually distinct death. this procedure is probably justifiable, but any legislation or policy regarding such cases should be flexible and should require consent.	1986	3957650
[beef-extract agar with sodium nucleinate as a medium for storage and stabilization of biological properties of shigella].		1986	3078175
structural identification of platelet activating factor in psoriatic scale.	platelet activating factor was isolated from the lesional scale of psoriatic patients using the method described by bligh and dyer (8). the extract was subjected to thin layer chromatography, and the region of the plate co-migrating with platelet activating factor removed. a portion of each sample was assayed for aggregating activity using washed guinea-pig platelets and the remainder treated with phospholipase c, derivatised, and subjected to reversed phase high performance liquid chromatograph ...	1985	3970690
spatial contrast sensitivity deficits in monkeys produced by optically induced anisometropia.	an anisometropia was simulated in infant rhesus monkeys by securing a high-powered minus lens (-10 d) in front of one eye. the anisometropia rearing procedure was initiated at 30 days of age and was continued for durations of 30, 60, or 90 days. behavioral measurements of spatial contrast sensitivity obtained when the animals were 9 months of age indicated that the monkeys treated for 30 days had equal or nearly equal contrast sensitivities and cut-off spatial frequencies in the two eyes. the 30 ...	1985	3972513
noninvasive measurement of pressure/volume characteristics in human limb veins by electrical admittance plethysmography.		1985	3974317
[persistent chlamydia infection in cell culture].		1985	3993192
[mechanism of the anti-arrhythmic action of phosphocreatine in acute myocardial ischemia].	the association between phosphocreatine's antifibrillatory action and its effect on the excitement propagation processes in the ischemic area was investigated under acute coronary arterial occlusion in dogs. ischemia considerably reduced the amplitude, and increased the duration and time of onset, in local electrograms, and provoked cardiac fibrillation at the time of occlusion or during the recovery of coronary flow. a single intravenous injection of 300 mg/kg phosphocreatine eliminated cardiac ...	1985	4021280
[ultrastructural aspects of the wound healing process of the skin of albino rats].		1985	4089424
the biologic significance of the mixed lymphocyte kidney culture in humans.	the mixed lymphocyte kidney culture (mlkc) in humans has been studied in normal and abnormal clinical conditions. human renal cortical cells were extracted by collagenase treatment from the kidneys of "normal" heart-beating cadaver organ donors (n = 13), patients with end-stage renal disease (esrd) at pretransplant bilateral nephrectomy and splenectomy (n = 13), and from irreversibly rejected renal allografts at the time of graft nephrectomy (n = 5). proliferation of peripheral blood t lymphocyt ...	1985	2996186
immunocytochemical studies of somatostatin neurons in brain.	immunohistochemical studies with antisera to somatostatin have, in many instances, led the way to our present understanding of the peptidergic nervous system. somatostatin was among the first of the hypophysiotropic hormones shown to be contained in diverse neuronal circuits outside of the hypothalamus. for example, somatostatin is found within neurons ranging in location from the cerebral cortex to primary sensory neurons to enteric neurons within the gut wall. somatostatin was also the first n ...	1985	2863929
protein loss of bovine dental enamel during in-vitro subsurface demineralization.	a chemical system based on the dialysis principle was used to study protein loss of dental enamel during demineralization with an acetic-acid buffer solution containing calcium and phosphate, in which the fluoride-ion activity was kept constant. this resulted in a subsurface lesion, with a depth of about 130 microns. after demineralization, protein material was isolated from the demineralization solution. u.v. spectra of the protein showed strong absorbance between 240 and 300 nm. amino-acid com ...	1985	3865639
role of the posterior capsule in the prevention of postoperative bacterial endophthalmitis: experimental primate studies and clinical implications.	the posterior capsule has an important effect on the risk of postoperative bacterial endophthalmitis. in order to investigate whether the posterior capsule inhibited the spread of infection into the vitreous we performed extracapsular cataract extraction in both eyes of 10 primates. in one eye of each primate the posterior capsule was left intact and in the other eye a large posterior capsulectomy was performed. when the anterior chambers were challenged with equivalent inocula of staphylococcus ...	1985	3877524
addition of three new serotypes of shigella boydii to the shigella schema.	no new serotypes have been added to the shigella schema since 1958, although several provisional serotypes have been described. we conducted biochemical and serological studies on three provisional shigella boydii serotypes. four strains of serotype 2710-54 from four widely separated countries, 7 strains of serotype 3615-53 from three different countries, and 31 strains of serotype 1344-78 (e10163) from six different countries were included. reactions of all three serotypes were consistent with ...	1985	3881469
plaque formation by virulent shigella flexneri.	an in vitro tissue culture plaque assay was developed to investigate the intracellular replication and intercellular spread of virulent shigellae. shigella plaques were formed in hela cell monolayers in the presence of an agarose overlay containing tissue culture medium and gentamicin, which eliminated extracellular bacterial growth. microscopically, the plaques were characterized by a central area of dead host cells surrounded by cells infected with shigellae. cells further away from the plaque ...	1985	3884506
studies of metabolites in diarrheal stool specimens containing shigella species by frequency-pulsed electron capture gas-liquid chromatography.	eleven diarrheal stool specimens and 10 control stool specimens from cairo, egypt, were studied by frequency-pulsed electron capture gas-liquid chromatography (fpec-glc). four cases involving shigella sonnei, three cases involving shigella boydii, and four cases involving shigella flexneri were studied. the aqueous stools were centrifuged, extracted with organic solvents, and derivatized to form specific electron-capturing derivatives of carboxylic acids, alcohols, hydroxy acids, and amines. ana ...	1985	3886695
[shigella serotypes isolated july-october 1983 and their antibiotic sensitivity].	twenty four strains of shigella were isolated from the stool of patients who had been admitted to the hospital with the complaint of diarrhea. of these, 17 were found to be sh. flexneri, and 7 sh. boydii. antibiotic susceptibility tests indicated that all of the 24 isolates probably had multiple resistance factors. twenty three of 24 strains were found to be resistant to ampicillin. this may be important from a therapeutic point of view.	1985	3892249
[detection of shigella antigens in the feces of patients with acute intestinal diseases using a coagglutination reaction].	a total of 113 patients with acute intestinal diseases have been examined with the use of the coagglutination test. 84.95% of the patients showed the presence of different shigella antigens. in patients with bacteriologically confirmed dysentery the corresponding shigella antigens were detected in 96.97% of cases in s. sonnei dysentery, in 90% of cases in s. flexneri dysentery, in 75% of cases in s. newcastle dysentery and in 100% of bases in s. boydii dysentery. in 81.6% of patients with acute ...	1985	3892986
epidemiology of shigella boydii serotype 2, a strain indigenous to spain.		1985	3899639
shigella septicemia in elderly patients.		1985	3899990
[3-year study of shigellosis epidemic in rwanda, central africa. problems of public health and bacteriological aspects].	since 1979, the lakes region (kivu in zaïre, western uganda, rwanda, burundi) has been stricken by a bacillary dysentery epidemic at s. dysenteriae type 1. supposed to be extinct since the 1950s., this bacillus has revealed a very noticeable virulence as well by the number of registered cases (524/100.000 in 1983 at ruhengeri-rwanda) as its fast break through. demographic density (from 400 to 600/km2) has played an important role and has facilitated the transition toward epidemicity. systematic ...	1985	3906345
determination of nanogram quantities of vanadium in biological material by isotope dilution thermal ionization mass spectrometry with ion counting detection.		1985	3907412
n-methyl-l-glutamate synthase.		1985	3911004
mechanism of sbcb-suppression of the recbc-deficiency in postreplication repair in uv-irradiated escherichia coli k-12.	the mechanism by which an sbcb mutation suppresses the deficiency in postreplication repair shown by recb recc mutants of escherichia coli was studied. the presence of an sbcb mutation in uvra recb recc cells increased their resistance to uv radiation. this enhanced resistance was not due to a suppression of the minor deficiency in the repair of dna daughter-strand gaps or to an inhibition of the production of dna double-strand breaks in uv-irradiated uvra recb recc cells; rather, the presence o ...	1985	3911022
photochemical modifications of lac repressor: effect of effectors binding on tryptophan photooxidation.		1985	3911222
[significance of isolating shigella under epidemiological circumstances].		1985	3911318
bactericidal properties of a new water disinfectant.	the n-chloramine compound 3-chloro-4,4-dimethyl-2-oxazolidinone (agent i) has been compared with calcium hypochlorite as to its efficacy as a bactericide for the treatment of water. the study included concentration, contact time, ph, temperature, and water quality as controlled variables. the species of bacteria tested were staphylococcus aureus, pseudomonas aeruginosa, and shigella boydii. in general, for highly pure, demand-free water, calcium hypochlorite was the more rapid disinfectant at a ...	1985	3922300
studies on microsomal azoreduction. n,n-dimethyl-4-aminoazobenzene (dab) and its derivatives.	the azoreduction of n,n-dimethyl-4-aminoazobenzene (dab) and n-methyl-4-amino-azobenzene (mab) by rat liver microsomes was investigated. it was shown that measurement of azoreduction of dab and structurally related azo dyes by the conventional method of substrate disappearance required an anaerobic environment since n-demethylated and ring-hydroxylated metabolites formed aerobically interfered with the assay system, producing quantitatively inaccurate results. oxygen partially, but not totally, ...	1985	3929790
identification of 5-acetamido-3,5,7,9-tetradeoxy-7-[(r)-3-hydroxybutyramido]-l- glycero-l-manno-nonulosonic acid as a component of bacterial polysaccharides.		1985	3933831
initiation and promotion in young and old animals. implications for human tumour formation.	the significance of ageing in experimental skin carcinogenesis and on the manifestation of tumour formation in man was studied. initiation with the carcinogenic hydrocarbon 7,12-dimethylbenz [a]anthracene and promotion with croton oil in mouse skin showed that tumour formation was unchanged when promotion was delayed 3, 10, 23 or 43 weeks in comparison with that in untreated animals of the same age. the sensitivity to carcinogen treatment of animals over 71 weeks of age was less than that of ani ...	1985	3938448
structural analysis of the o-glycosidically linked core-region oligosaccharides of human meconium glycoproteins which express oncofoetal antigens.	glycoproteins were extracted from meconium samples of group o neonates of secretor type by pronase digestion followed by precipitation in 67% aqueous ethanol and separated into ii antigen enriched and depleted fractions by affinity chromatography. the latter fraction strongly expressed the oncofoetal antigens recognised by natural antibodies in mouse sera and the hybridoma antibody fc 10.2, and this activity was enhanced after mild acid hydrolysis to remove sialic acid and fucose residues. oligo ...	1985	2580706
comparison of minimum inhibitory concentration of water soluble extracts of eugenia jambolana lam. (fam. myrtaceae) barks of different ages on dysentery and diarrhoea forming micro - organisms.	a preliminary investigations was carried out to study the antibacterial activity of the water soluble extracts of five and ten years old barks of eugenia jambolana lam. (fam. myrtaceae) on dysentery and diarrhoea forming micro organisms. it was observed that the barks of young plants have a better inhibitory effect on micro - organisms like salmonella viballerup, shigella dysenteriae 10, shigella boydii 5, sgigella dysenteriae 2.	1985	22557509
the anatomy of a smile.	the anatomy of the smile is an integral part of dentistry. its understanding involves close scrutiny of all elements of the oral region. it is not enough to establish the size of teeth based on the high and low lip lines, size of the mouth, and a shade to blend with the age and complexion. to create a harmonious smile the dentist must maintain or create the normal curvature of the lips, proper exposure of the red zone of the lips, an undistorted philtrum, and undisturbed nasolabial grooves. thes ...	1985	271728
[effects of different postures on left ventricular function during various trimesters of pregnancy].		1985	261924
[evaluation of various radiographic technics for the diagnosis of periodontal diseases with special reference to the status x unilateral technic].	various x-ray techniques (panorama views and intraoral plates) were contrasted and evaluated for diagnostic use in periodontal lesions. in addition the status x-unilateral technique (durner, meyer) was compared to intraoral right angle roentgen states (updergrave) in the examination of 20 patients.	1985	271590
percutaneous transluminal coronary angioplasty.		1985	373562
effects of saliva and alpha-amylase on antibiotic sensitivity of bacteria.	two hundred and ninety-six bacterial isolates were investigated for the effects of saliva and alpha-amylase on their susceptibility to ampicillin, tetracycline, chloramphenicol and gentamicin. when the test organisms were primed with normal and 'diseased' saliva there were no observable differences in the mics of ampicillin and chloramphenicol for group-a streptococci, but alpha-amylase significantly reduced the mic of tetracycline from 2 to 0.25 mg/1. with staphyloccus aureus, priming with sali ...	1984	6087634
shigella dysentery in tripoli, libya.		1984	6371260
an outbreak of shigellosis in an ultra-orthodox jewish community.	an outbreak of shigellosis due to shigella sonnei, s. flexnerii and s. boydii in an ultra-religious jewish community in southern israel is described. the source of epidemic was traced to vegetables bought from a single source. the importance of epidemic and endemic modes of spread of shigella is discussed.	1984	6379888
[clinical characteristics of dysentery in relation to the oxidation-reduction features of isolated shigella].		1984	6379895
[bacillary dysentery 1982].		1984	6390523
[clinical and treatment characteristics of acute dysentery caused by shigella boydii and newcastle].		1984	6390958
[conditions and factors determining the etiological structure of dysentery and its alterations. ii. concepts, hypotheses and points of view yesterday and today].		1984	6391040
[species of shigella isolated in a 4-year period and antimicrobial sensitivity].		1984	6395307
[structure of the causative agents of shigellosis in areas of the ussr 1979-1981].	summarized data on the specific structure of shigella strains isolated in different territories of the ussr in 1979-1981 are presented. these data indicate that in the intra-specific structure of s. flexneri strains the proportion of serological subvariant 2a increased and that of serological variant 6 and subvariants 16 and 4a decreased during this period. using one of the base territories of the all-union shigellosis center as an example, the spread of an unusual serological variant of s. flex ...	1984	6395590
drug resistance in shigella dysenteriae, s flexneri and s boydii in england and wales: increasing incidence of resistance to trimethoprim.	a total of 2753 strains of shigella belonging to subgroups a, b, and c that were isolated from patients in england and wales during the period from 1979 to mid-1983 were studied. of these, 1690 (61%) were from patients recently returned from abroad or in contact with recent travellers, and 760 (45%) of these affected travellers from the indian subcontinent. the number of strains resistant to sulphonamides and streptomycin remained at a high level throughout (average 76% and 72% respectively). re ...	1984	6423079
sialic acids of a new type from the lipopolysaccharides of pseudomonas aeruginosa and shigella boydii.		1984	6437679
[a strain of shigella boydii serovar 18 isolated in japan from a diarrhea patient after a trip to southeast asia].		1984	6442723
[shigellosis abroad].		1983	6379992
[bacterial antigenic polysaccharides. 12. structure and 13c nmr spectrum of the polysaccharide chain of shigella boydii type 8 lipopolysaccharide].	a specific acidic polysaccharide was isolated from sh. boydii type 8 antigenic lipopolysaccharide after mild hydrolysis followed by chromatography on sephadex g-50. the polysaccharide consists of d-glucuronic acid, d-galacturonic acid, 2-acetamido-2-deoxy-d-glucose, 2-acetamido-2-deoxy-d-galactose and 2-amino-1,3-propanediol residues in 1:1:1:1:1 ratio. from the results of methylation analysis, partial acid hydrolysis and smith degradation, the structure of the repeating unit of the specific pol ...	1983	6207837
cell-wall lipopolysaccharide of escherichia coli 0114:h2. structure of the polysaccharide chain.	the o-specific polysaccharide of the 0114 antigen (lipopolysaccharide) of escherichia coli 0114 and oligosaccharides obtained from it by smith degradation and hydrogen fluoride solvolysis were analyzed, using proton and 13c nuclear magnetic resonance spectroscopy and methylation. the results indicated that the 0114 polysaccharide has the tetrasaccharide repeating unit alpha-n-acetylglucosamine(1 leads to 4) beta-3,6-dideoxy-3-(n-acetyl-l-seryl)aminoglucose(1 leads to 3) beta-ribofuranose(1 leads ...	1983	6309515
enterotoxigenic and enteroinvasive escherichia coli strains belonging to a new o group, o167.	a new escherichia coli o group, o167, is described. its o antigen is related to that of shigella boydii 3. a total of 29 strains belonging to the new o group were examined. three strains were determined to be invasive by the sereny test and by tissue culture tests and were non-enterotoxigenic. all three were nonmotile and failed to decarboxylate lysine. a total of 26 strains were noninvasive. of these, 25 were motile, 24 decarboxylated lysine, and 19 were enterotoxigenic, 17 producing heat-stabl ...	1983	6341404
[epidemiology of bacillary dysentery in algeria. i. the epidemiological aspects of dysentery in algeria].	bacterial dysentery plays an important role among infectious diseases in algeria. a tendency to the growth of the morbidity rate has been observed. dysentery is irregularly spread in different zones of the country. the highest morbidity rate is registered in the sahara zone and in the eastern part of the country. the morbidity rate among the urban population is higher than among the rural population. s. flexneri prevail in the etiological structure of dysentery infection. of all age groups, the ...	1983	6342313
[trends in the spread of boyd 4 dysentery and the characteristics of its clinical picture and of the biology of its causative agent].	the data on the proportion of s. boydii 4 in the general structure of the causative agents of dysentery, as well as in the intraspecific structure of s. boydii, in some areas of the ussr in 1977-1981 are presented. s. boydii strains 4, circulating in one of the areas where their proportion considerably increased in 1980-1981, corresponded to their taxonomic position in their biological properties, while forming a single biochemical variant according to the character of the fermentation of sorbit ...	1983	6356722
molecular comparison of virulence plasmids in shigella and enteroinvasive escherichia coli.	virulent isolates of shigella dysenteriae and shigella boydii harboured a 140 mdal plasmid which was either absent or deleted in spontaneously avirulent strains. together with previous data concerning s. sonnei, s. flexneri and enteroinvasive escherichia coli, the present results established the general role of extrachromosomal elements in the virulence of such enteroinvasive species. among different species, these virulence plasmids showed unrelated endonuclease cleavage patterns, whereas hybri ...	1983	6357023