Publications

rapid identification of gram-negative bacteria with and without ctx-m extended-spectrum ß-lactamase from positive blood culture bottles by pcr followed by microchip gel electrophoresis.	we evaluated the usefulness of pcr analysis of the 16s-23s rrna gene internal transcribed spacer (its) and the ctx-m extended-spectrum ß-lactamase (esbl) followed by microchip gel electrophoresis (mge) for direct identification and ctx-m detection of gram-negative bacteria (gnb) from positive blood culture bottles. of 251 gnb isolated from blood cultures containing a single bacterium, 225 (90%) were correctly identified at the species level directly from positive blood culture bottles by compari ...	2011	21289149
unusual cause of cerebral vasospasm after pituitary surgery.	cerebral vasospasm (cvs) was described in patients after trans-sphenoidal pituitary surgery due to intra-operative trauma of arteries or blood clots around the arteries of willis' circle. we consider that in the two presented cases the main cause of cvs in early postoperative period was meningitis. two patients with pituitary adenomas were operated with trans-sphenoidal approach. cvs developed in early postoperative period. meningitis was revealed in both cases. cvs regressed only after successf ...	2011	21290157
in vitro and in vivo properties of bal30376, a β-lactam and dual beta-lactamase inhibitor combination with enhanced activity against gram-negative bacilli that express multiple β-lactamases.	bal30376 is a triple combination comprising a siderophore monobactam, bal19764; a novel bridged monobactam, bal29880, which specifically inhibits class c β-lactamases; and clavulanic acid, which inhibits many class a and some class d β-lactamases. the mic(90) was ≤ 4 μg/ml (expressed as the concentration of bal19764) for most species of the enterobacteriaceae family, including strains that produced metallo-β-lactamases and were resistant to all of the other β-lactams tested. the mic(90) for sten ...	2011	21245441
international transfer of ndm-1-producing klebsiella pneumoniae from iraq to france.		2011	21245442
experimental phage therapy in treating klebsiella pneumoniae-mediated liver abscesses and bacteremia in mice.	intragastric inoculation of mice with klebsiella pneumoniae can cause liver abscesses, necrosis of liver tissues, and bacteremia. a newly isolated phage (φnk5) with lytic activity for k. pneumoniae was used to treat k. pneumoniae infection in an intragastric model. both intraperitoneal and intragastric administration of a single dose of φnk5 lower than 2 × 10(8) pfu at 30 min after k. pneumoniae infection was able to protect mice from death in a dose-dependent manner, but the efficacy achieved w ...	2011	21245450
intravenous colistin administration in neonates.	nosocomial infection due to multidrug-resistant gram-negative pathogens in intensive care units is a challenge for clinicians and microbiologists, and has led to resurgence of parenteral colistin use in the last decade. safety and efficacy data regarding intravenous colistin (colistimethate) use in neonates is sparse. we present our experience of efficacy and safety of colistimethate in the treatment of sepsis in critically sick term and preterm neonates.	2011	21245777
an in vitro comparative study determining bactericidal activity of stabilized chlorine dioxide and other oral rinses.	the study was conducted to determine the bactericidal activity of a stabilized chlorine dioxide oral rinse (closys oral rinse) compared to products currently available on the market.	2011	21290979
new delhi metallo-beta-lactamase, ontario, canada.		2011	21291614
pandrug-resistant isolate of klebsiella pneumoniae causes less damage than drug-susceptible isolates in a rabbit model.	bacterial infections induce a series of inflammatory responses and lead to longer hospital stays and increased mortality. in clinical work, we often find that infections caused by drug-susceptible isolates have a worse outcome than those caused by pandrug-resistant isolates. to goal of this study was to assess the impact of drug resistant in a rabbit model of klebsiella pneumoniae infection.	2011	21291634
thrombocytes are effectors of the innate immune system releasing human beta defensin-3.	thrombocyte concentrate i.e. platelet-rich plasma (prp) has become a popular adjunct for many surgical procedures. it is believed to improve bone and soft tissue healing. recently antimicrobial effects of the autologous preparation were reported by several groups. in this study we investigated the antimicrobial effect of prp against gram-negative microbes which frequently cause severe complications in orthopaedic trauma surgery.	2011	21310405
bacterial species associated with sound and botrytis-infected grapes from a greek vineyard.	grape bacterial microbiota plays central roles in the quality of grapes and wine, yet its diversity remains poorly described. in the present study, bacterial species associated with sound and botrytis-infected grapes of two cultivars originating from the same vineyard were assessed. isolates were identified by pcr-denaturing gradient gel electrophoresis (pcr-dgge) and sequence analysis of partial 16s rrna gene. comparable counts were recorded between botrytis-infected and sound grape samples. in ...	2011	21315469
antimicrobial drug resistance in taiwan.	antimicrobial resistance is a major global health threat associated with high mortality rates and high medical costs. geographic variations in resistance profiles of bacterial and fungal pathogens have had a considerable impact on antimicrobial prescription. in taiwan, there is an alarmingly high prevalence of penicillin-resistant streptococcus pneumoniae, multidrug-resistant and extensively drug-resistant (xdr) pseudomonas aeruginosa and acinetobacter baumannii, extended-spectrum ß-lactamase-pr ...	2011	21316007
molecular epidemiology and resistance mechanisms involved in reduced susceptibility to amoxicillin/clavulanic acid in klebsiella pneumoniae isolates from a chronic care centre.	the aim of this work was to investigate the molecular epidemiology and mechanisms responsible for reduced susceptibility to amoxicillin/clavulanic acid (amc) amongst cefazolin-susceptible klebsiella pneumoniae isolates from patients admitted to a chronic care institution. in total, 51 (29.8%) of 171 k. pneumoniae isolates recovered between 2006 and 2008 were non-susceptible to amc, of which 45 were susceptible to cefazolin. nucleotide sequencing analysis revealed that 19 produced irt-11 and the ...	2011	21316198
synthesis, stereochemistry, antimicrobial evaluation and qsar studies of 2,6-diaryltetrahydropyran-4-one thiosemicarbazones.	a series of 2,6-diaryltetrahydropyran-4-one thiosemicarbazones (11-27) were synthesized and characterized for evaluation of potential antibacterial activity against staphylococcus aureus, escherichia coli, pseudomonas aeruginosa, salmonella typhi, bacillus subtilis and klebsiella pneumonia and antifungal activity against cryptococcus neoformans, candida albicans, rhizopus sp., aspergillus niger and aspergillus flavus were evaluated. compounds 21 and 22 showed maximum inhibition potency at low co ...	2011	21316819
emergence of klebsiella pneumoniae st258 with kpc-2 in hong kong.		2011	21316928
containment of a country-wide outbreak of carbapenem-resistant klebsiella pneumoniae in israeli hospitals via a nationally implemented intervention.	during 2006, israeli hospitals faced a clonal outbreak of carbapenem-resistant klebsiella pneumoniae that was not controlled by local measures. a nationwide intervention was launched to contain the outbreak and to introduce a strategy to control future dissemination of antibiotic-resistant bacteria in hospitals.	2011	21317398
enhancement of 2,3-butanediol production by klebsiella oxytoca ptcc 1402.	optimal operating parameters of 2,3-butanediol production using klebsiella oxytoca under submerged culture conditions are determined by using taguchi method. the effect of different factors including medium composition, ph, temperature, mixing intensity, and inoculum size on 2,3-butanediol production was analyzed using the taguchi method in three levels. based on these analyses the optimum concentrations of glucose, acetic acid, and succinic acid were found to be 6, 0.5, and 1.0 (% w/v), respect ...	2011	21318172
splenic abscess: a single institution study and review of the literature.	the aim of this study was to review our experience with splenic abscesses, with respect to the relevant aspects of splenic abscesses and treatment outcomes.	2011	21319348
transport and bacterial interactions of three bacterial strains in saturated column experiments.	the impact of bacteria-solid and bacteria-bacteria interactions on the transport of klebsiella oxytoca, burkholderia cepacia g4pr1, and pseudomonas sp. #5 was investigated in saturated sand column experiments (l = 114 mm; ø = 33 mm) under constant water velocities (~ 5 cm · h(-1)). bacterial strains were injected into the columns as pulses either individually, simultaneously, or successively. a one-dimensional mathematical model for advective-dispersive transport and for irreversible and reversi ...	2011	21319738
rapid genotyping of achromobacter xylosoxidans, acinetobacter baumannii, klebsiella pneumoniae, pseudomonas aeruginosa and stenotrophomonas maltophilia isolates using melting curve analysis of rapd-generated dna fragments (mcrapd).	typing of bacteria is important for monitoring newly emerging pathogens and for examining local outbreaks. we evaluated the randomly amplified polymorphic dna technique in combination with melting curve analysis (mcrapd) of the amplified dna fragments to genotype isolates from five gram-negative species, i.e. achromobacter xylosoxidans, acinetobacter baumannii, klebsiella pneumoniae, pseudomonas aeruginosa and stenotrophomonas maltophilia. by determining the melting temperature peaks of the ampl ...	2011	21320595
high prevalence of kpc-2-type carbapenemase coupled with ctx-m-type extended-spectrum beta-lactamases in carbapenem-resistant klebsiella pneumoniae in a teaching hospital in china.		2011	21321140
in vitro interactions of antimicrobial combinations with fosfomycin against kpc-2-producing klebsiella pneumoniae and protection of resistance development.	using time-kill methodology, we investigated the interactions of fosfomycin with meropenem or colistin or gentamicin against 17 genetically distinct klebsiella pneumoniae clinical isolates carrying blakpc-2. synergy was observed with meropenem or colistin against 64.7 and 11.8% of tested isolates, while the combination with gentamicin resulted in indifference. all studied combinations showed improved bactericidal activity, compared to fosfomycin alone and prevented the development of fosfomycin ...	2011	21321144
antimicrobial activity of cxa-101, a novel cephalosporin tested in combination with tazobactam against enterobacteriaceae, pseudomonas aeruginosa, and bacteroides fragilis strains having various resistance phenotypes.	cxa-101, a novel oxyimino-aminothiazolyl cephalosporin, cxa-201 (cxa-101 combined with tazobactam), and various comparators were susceptibility tested by broth microdilution methods against 1,301 well-characterized clinical strains collected worldwide, including ceftazidime-resistant members of the family enterobacteriaceae and klebsiella pneumoniae carbapenemase (kpc)- and extended-spectrum ß-lactamase (esbl)-producing strains of pseudomonas aeruginosa and bacteroides fragilis. cxa-201 was 2- t ...	2011	21321149
mutation in fucose synthesis gene of klebsiella pneumoniae affects capsule composition and virulence in mice.	the emerging pathogenicity of klebsiella pneumoniae (kp) is evident by the increasing number of clinical cases of liver abscess (la) due to kp infection. a unique property of kp is its thick mucoid capsule. the bacterial capsule has been found to contain fucose in kp strains causing la but not in those causing urinary tract infections. the products of the gmd and wcag genes are responsible for converting mannose to fucose in kp. a kp strain, kpl1, which is known to have a high death rate in infe ...	2011	21321319
bilateral klebsiella pneumoniae (k1 serotype) endogenous endophthalmitis as the presenting sign of disseminated infection.	the authors report a rare presentation of bilateral klebsiella pneumoniae endophthalmitis in an ambulatory patient without other known medical conditions. a 51-year-old chinese woman presented with severe bilateral reduction in vision, photophobia, pain, and eyelid swelling. hospital admission and evaluation revealed vitreous, blood, and urine cultures positive for k. pneumoniae (k1 serotype). additional work-up revealed endocarditis, multiple liver abscesses, brain abscesses, and left lobar pne ...	2011	21323188
comparison of in vitro activity of ertapenem with other carbapenems against extended-spectrum beta-lactamase-producing escherichia coli and kleibsella species isolated in a tertiary children's hospital.	the purpose of this study was to evaluate the susceptibility of extended-spectrum beta-lactamase (esbl)-producing enterobacteriaceae clinical isolates to ertapenem in a tertiary pediatric care center in turkey.	2011	21323503
assessing the effect of different ultrasonic frequencies on bacterial viability using flow cytometry.	aims: this research investigated the effect of sonication at frequencies of 20, 40 and 580 khz and approximately the same acoustic intensity on the viability and declumping of two micro-organisms (escherichia coli and klebsiella pneumonia). methods and results: two analytical methods were employed; viable plate counts (cfu ml(-1) ) and flow cytometry to identify and quantify both live/viable and dead bacteria in the bulk liquid. flow cytometry results for e. coli and kl. pneumonia indicated a ...	2011	21324052
sequence analysis of a group of low molecular-weight plasmids carrying multiple is903 elements flanking a kanamycin resistance aph gene in salmonella enterica serovars.	a group of low molecular-weight cole1-like plasmids carrying the aph sequence type aph(ii) from three different salmonella serovars were sequenced. these plasmids carry two or more copies of is903 elements, with up to 21bp sequence differences to one another, two of which flank the aph gene. this group of plasmids did not appear to carry any known mobilization genes and instead carry three open reading frames encoding hypothetical proteins of unknown function possibly organized in an operon. the ...	2011	21324339
time-kill synergy tests of tigecycline combined with imipenem, amikacin, and ciprofloxacin against clinical isolates of multidrug-resistant klebsiella pneumoniae and escherichia coli.	this study evaluated the activity of tigecycline combined with imipenem, amikacin, and ciprofloxacin against clinical isolates of multidrug-resistant klebsiella pneumoniae and escherichia coli co-producing extended-spectrum ß-lactamases and acquired ampc ß-lactamases. broth microdilution tests were performed for cefotaxime, ceftazidime, cefepime, imipenem, amikacin, ciprofloxacin, and tigecycline. time-kill synergy studies were tested for tigecycline plus imipenem, tigecycline plus amikacin, and ...	2011	21325253
new delhi metallo-ß-lactamase (ndm-1)-producing klebsiella pneumoniae: case report and laboratory detection strategies.	the spread of antimicrobial resistance among enterobacteriaceae is a significant clinical threat. we report the first case of an enterobacteriaceae strain harboring the ndm-1 metallo-ß-lactamase in a pediatric patient in the united states. we describe strategies for the detection of this novel resistance mechanism encountered in an isolate of klebsiella pneumoniae.	2011	21325558
isolation and characterisation of kp34--a novel fkmv-like bacteriophage for klebsiella pneumoniae.	bacteriophage kp34 is a novel virus belonging to the subfamily autographivirinae lytic for extended-spectrum ß-lactamase-producing klebsiella pneumoniae strains. its biological features, morphology, susceptibility to chemical and physical agents, burst size, host specificity and activity spectrum were determined. as a potential antibacterial agent used in therapy, kp34 molecular features including genome sequence and protein composition were examined. phylogenetic analyses and clustering of kp34 ...	2011	21327407
isolation of a selected microbial consortium capable of degrading methyl parathion and p-nitrophenol from a contaminated soil site.	a bacterial consortium with the ability to degrade methyl parathion and p-nitrophenol, using these compounds as the only carbon source, was obtained by selective enrichment in a medium with methyl parathion. samples were taken from moravia, medellin; an area that is highly contaminated, owing to the fact that it was used as a garbage dump from 1974 to 1982. acinetobacter sp, pseudomonas putida, bacillus sp, pseudomonas aeruginosa citrobacter freundii, stenotrophomonas sp, flavobacterium sp, prot ...	2011	21328125
extended-spectrum ß-lactamases of escherichia coli and klebsiella pneumoniae screened by the vitek 2 system.	the vitek 2 automated system (biomérieux) is one of the most widely used instruments in clinical microbiology laboratories for the identification and evaluation of the susceptibility profiles of bacteria including the detection of extended-spectrum ß-lactamases (esbls) produced by escherichia coli, klebsiella pneumoniae and klebsiella oxytoca. currently, the clinical and laboratory standards institute recommends the use of esbl confirmatory tests in addition to standard susceptibility testing. i ...	2011	21330411
acute klebsiella pneumoniae interface keratitis after deep anterior lamellar keratoplasty.		2011	21331700
phenotypic and genotypic screening and clonal analysis of carbapenem-resistant klebsiella pneumoniae at a single hospital.	detection of bla(kpc)-harboring klebsiella pneumoniae (kp) in the clinical laboratory remains a difficult task. decreased ertapenem (ert) susceptibility has been considered one of the most sensitive phenotypic indicators of k. pneumoniae carbapenemase (kpc) production, but has been found to be nonspecific. susceptibility testing using imipenem or meropenem lacks the sensitivity for detection of kpcs, and there is limited experience using doripenem (dor). fifty-five individual ert-nonsusceptible ...	2011	21332362
[microbiological profile of infections in the intensive care units of colombia (episepsis colombia].	valid and reliable data regarding sepsis is lacking in colombia. our aim was to determine the prevalence of the microorganisms in the main infections treated in intensive care units (icus) in our country.	2011	21334104
symptomatic secondary vesical calculus formed on an intrauterine contraceptive device inserted 25 years previously.	a 57-year-old postmenopausal woman presented with vague lower abdominal symptoms, dysuria and recurrent urinary tract infection of a year's duration. she had an intrauterine contraceptive device (iucd) inserted 25 years previously and denied having any significant gynecological or urinary tract symptoms since the device was inserted. ct scan of her pelvis confirmed the presence of an iucd that had migrated into the urinary bladder and on which a calculus had formed. an attempt at removal of the ...	2011	21335943
degradation of tannic acid by cold-adapted klebsiella sp nacasa1 and phytotoxicity assessment of tannic acid and its degradation products.	the focus of the present study is to know the potential of bacterial isolate for tannic acid degradation at low temperature. also, we tried to evaluate the suitability of phytotoxicity testing protocol for the determination of tannic acid toxicity.	2011	21336632
development of recombinant klebsiella pneumoniae ∆dhat strain for the co-production of 3-hydroxypropionic acid and 1,3-propanediol from glycerol.	klebsiella pneumoniae converts glycerol to the specialty chemical 1,3-propanediol (1,3-pdo), which is used for the production of polytrimethylene terepthalate (ptt). in this study, an nad(+)-dependent gamma-glutamyl-gamma-aminobutyraldehyde dehydrogenase (puuc) of k. pneumoniae dsm 2026, which oxidizes 3-hydroxypropionaldehyde to a platform chemical 3-hydroxypropionic acid (3-hp), was cloned and overexpressed in k. pneumoniae dsm 2026 for the co-production of 3-hp and 1,3-pdo from glycerol. in a ...	2011	21336929
molecular characterization of antimicrobial resistance in gram-negative bacteria isolated from bovine mastitis in egypt.	the aim of this study was to characterize the genetic basis of multidrug resistance in gram-negative bacteria isolated from bovine mastitis cases in egypt. multidrug resistance phenotypes were found in 34 of 112 (30.4%) gram-negative bacterial isolates, which harbored at least one antimicrobial resistance gene. the most prevalent multidrug-resistant (mdr) species were enterobacter cloacae (8 isolates, 7.1%), klebsiella pneumoniae (7 isolates, 6.3%), klebsiella oxytoca (7 isolates, 6.3%), escheri ...	2011	21338385
sorting of an integral outer membrane protein via the lipoprotein-specific lol pathway and a dedicated lipoprotein pilotin.	the lipoprotein puls is a dedicated chaperone that is required to target the secretin puld to the outer membrane in klebsiella or escherichia coli, and to protect it from proteolysis. here, we present indirect evidence that puld protomers do not assemble into the secretin dodecamer before they reach the outer membrane, and that puls reaches the outer membrane in a soluble heterodimer with the general lipoprotein chaperone lola. however, we could not find any direct evidence for puld protomer ass ...	2011	21338419
riding the trojan horse: combating pest insects with their own symbionts.	insects form an extremely large group of animals and bear a consequently large variety of associated microbes. this microbiota includes very specific and obligate symbionts that provide essential functions to the host, and facultative partners that are not necessarily required for survival. the tephritidae is a large family that includes many fruit pests such as the mediterranean fruit fly (the medfly, ceratitis capitata) and the olive fly (bactrocera oleae). community and functional analyses sh ...	2011	21338477
a new cost-effective, selective and differential medium for the isolation of cronobacter spp.	the objective of the present study was to develop a new selective, differential and cost-effective medium (kim and rhee - kr-medium) for the isolation of cronobacter spp. in this new medium, which contained salicin as a differential agent, cronobacter spp. generated typical colonies with characteristic violet-colored centers surrounded by a transparent to opalescent border, and the growth of other microorganisms (40 strains) was inhibited or produced visually distinguishable colonies. using heal ...	2011	21338629
investigation of pulmonary infection pathogens in neurological intensive care unit.	the purpose of this study is to investigate the distribution and antimicrobial susceptibility of pathogenic bacteria in inpatients with pulmonary infection in the neurological intensive care unit (nicu).	2011	21339939
[investigation of the susceptibilities of extended-spectrum beta-lactamase-producing escherichia coli and klebsiella spp. strains to ertapenem and other carbapenems].	infections caused by extended-spectrum beta-lactamase (esbl) producing escherichia coli and klebsiella spp. constitute severe problems. carbapenems are commonly used to treat these infections. however, infections caused by carbapenem-resistant gram-negative bacteria show an increasing trend recently. the aim of this study was to investigate the susceptibilities of esbl-producing e.coli and klebsiella spp. to ertapenem and other carbapenems. a total of 239 e.coli, 28 k.pneumoniae and 11 k.oxytoca ...	2011	21341156
[causative agents of intravenous catheter-related infections and their antibiotic susceptibilities].	intravenous catheterization can lead to colonization as well as a broad spectrum of infections ranging from catheter site infections to catheter-related blood stream infections (crbsis). the aim of this study was to evaluate the distribution of causative agents and their antibiotic susceptibility patterns in crbsis and catheter site infections along with the colonization rates and colonizing microorganisms in zonguldak karaelmas university hospital, turkey. the results of cultures from catheter ...	2011	21341163
[escherichia coli-induced emphysematous pyelonephritis in a diabetic patient with polycystic kidney disease].	emphysematous pyelonephritis is a rare, necrotizing infection of the kidney and the perirenal space resulting in the formation of gas in both structures and associated with a high mortality rate. in 90% of cases it affects one kidney only; in the remaining 10% with bilateral emphysematous pyelonephritis aggressive surgical intervention may be required. women are much more frequently affected than men, with diabetes mellitus (in 70-90% of cases) and urinary tract obstruction being common predispo ...	2011	21341250
a comparative study of the antimicrobial and phytochemical properties between outdoor grown and micropropagated tulbaghia violacea harv. plants.	the study aimed to compare the antimicrobial and phytochemical properties of in vitro cultured and outdoor grown tulbaghia violacea plants in the quest to validate the use of micropropagated plants as alternatives to outdoor grown plants in traditional medicine. tulbaghia violacea is used extensively in south african traditional medicine for hiv/aids patients and in the treatment of gastrointestinal ailments, asthma, fever and tuberculosis.	2011	21291985
the prevention of biofilm colonization by multidrug-resistant pathogens that cause ventilator-associated pneumonia with antimicrobial-coated endotracheal tubes.	ventilator-associated pneumonia (vap) continues to be the nosocomial infection associated with the highest mortality in critically ill patients. since silver-coated endotracheal tubes (ett) was shown in a multicenter prospective randomized trials to decrease the risk of vap, we compared the efficacy of two antiseptic agents such as gardine- and gendine-coated etts with that of silver-coated etts in preventing biofilm. the etts were tested for their ability to prevent the biofilm formation of met ...	2011	21295343
prevalence and phenotypic characterisation of carbapenem resistance in enterobacteriaceae bloodstream isolates in a tertiary care hospital in india.		2011	21295949
altering the composition of caseicins a and b as a means of determining the contribution of specific residues to antimicrobial activity.	caseicin a (ikhqglpqe) and caseicin b (vlnenllr) are antimicrobial peptides generated through the bacterial fermentation of sodium caseinate, and on the basis of this and previous studies, they are active against many gram-negative pathogens (cronobacter sakazakii, cronobacter muytjensii, salmonella enterica serovar typhimurium, escherichia coli, klebsiella pneumoniae, and pseudomonas fluorescens) as well as the gram-positive organism staphylococcus aureus. here we describe further studies with ...	2011	21296933
an experimentally validated genome-scale metabolic reconstruction of klebsiella pneumoniae mgh 78578, iyl1228.	klebsiella pneumoniae is a gram-negative bacterium of the family enterobacteriaceae that possesses diverse metabolic capabilities: many strains are leading causes of hospital-acquired infections that are often refractory to multiple antibiotics, yet other strains are metabolically engineered and used for production of commercially valuable chemicals. to study its metabolism, we constructed a genome-scale metabolic model (iyl1228) for strain mgh 78578, experimentally determined its biomass compos ...	2011	21296962
synthesis, characterization and biological evaluation of mononuclear co(ii), ni(ii), cu(ii) and pd(ii) complexes with new n2o2 schiff base ligands.	new tetradentate n(2)o(2) donor schiff bases and their mononuclear co(ii), ni(ii), cu(ii), and pd(ii) complexes were synthesized and characterized extensively by ir, (1)h-, (13)c-nmr, mass, esr, conductivity measurements, elemental and thermal analysis. specifically the magnetic and electronic spectral measurements demonstrate the octahedral structures of cobalt(ii), nickel(ii) complexes and square planar geometries of copper(ii), palladium(ii) complexes. all the ligands and complexes were scree ...	2011	21297294
antibacterial activity of the indigenous earthworms lampito mauritii (kinberg) and perionyx excavatus (perrier).	earthworms respond to microbial infection through cellular and humoral defense mechanisms such as antimicrobial protein secretions. most of the humoral defense proteins are synthesized in the skin itself.	2011	21299431
a multicentre surveillance study on the characteristics, bacterial aetiologies and in vitro antibiotic susceptibilities in patients with acute exacerbations of chronic bronchitis.	antimicrobial resistance is a global problem and the prevalence is high in many asian countries.	2011	21299688
multiresistant gram-negative bacteria: the role of high-risk clones in the dissemination of antibiotic resistance.	multilocus sequence typing reveals that many bacterial species have a clonal structure and that some clones are widespread. this underlying phylogeny was not revealed by pulsed-field gel electrophoresis, a method better suited to short-term outbreak investigation. some global clones are multiresistant and it is easy to assume that these have disseminated from single foci. such conclusions need caution, however, unless there is a clear epidemiological trail, as with kpc carbapenemase-positive kle ...	2011	21303394
first case of resistance to tigecycline by klebsiella pneumoniae in a european university hospital.		2011	21304206
act-6, a novel plasmid-encoded class c ß-lactamase in a klebsiella pneumoniae isolate from china.	the purpose of this study was to investigate the phenotypic and molecular characterization of a novel plasmid-mediated ampc-type ß-lactamase in klebsiella pneumoniae e701 isolated from anhui province in china. in comparison with the act-1, sequence analysis revealed that there were 43 point mutations in the coding gene, and 10 of which led to amino-acid substitution. resistance could be transferred by conjugation or transformation with plasmid dna into e. coli jm109, which was due to the product ...	2011	21304534
identification of pantoea ananatis gene encoding membrane pyrroloquinoline quinone (pqq)-dependent glucose dehydrogenase and pqqabcdef operon essential for pqq biosynthesis.	pantoea ananatis accumulates gluconate during aerobic growth in the presence of glucose. computer analysis of the p. ananatis sc17(0) sequenced genome revealed an orf encoding a homologue (named gcd) of the mgdh (ec 1.1.99.17) apoenzyme from escherichia coli and a putative pyrroloquinoline quinone (pqq) biosynthetic operon homologous to pqqabcdef from klebsiella pneumoniae. construction of ?gcd and ?pqq mutants of p. ananatis confirmed the proposed functions of these genetic elements. the p. ana ...	2011	21306430
antibiotic adjuvants: multicomponent anti-infective strategies.	the unremitting emergence of multidrug-resistant bacterial pathogens highlights a matching need for new therapeutic options. for example, new carbapenemases such as kpc (class a klebsiella pneumoniae) and ndm-1 (new delhi metallo-ß-lactamase 1) are surfacing, resulting in almost total resistance to ß-lactam antibiotics. furthermore, resistance is quickly disseminated, not only in the healthcare sector, but also within the community at large, because many resistance determinants are carried on mo ...	2011	21342612
outbreak of oxa-48-positive carbapenem-resistant klebsiella pneumoniae isolates in france.	seventeen klebsiella pneumoniae isolates producing the oxa-48 carbapenemase, obtained from 10 patients hospitalized from april to june 2010, mostly in the medical intensive care unit of the villeneuve-saint-georges hospital in a suburb of paris, france, were analyzed. seven patients were infected, of whom five were treated at least with a carbapenem, and five patients died. molecular analysis showed that the isolates belonged to a single clone that harbored a 70-kb plasmid carrying the blaoxa-48 ...	2011	21343451
multistep resistance development studies of ceftaroline in gram-positive and -negative bacteria.	ceftaroline, the active component of the prodrug ceftaroline fosamil, is a novel broad-spectrum cephalosporin with bactericidal activity against gram-positive and -negative isolates. this study evaluated the potential for ceftaroline and comparator antibiotics to select for clones of streptococcus pneumoniae, streptococcus pyogenes, haemophilus influenzae, moraxella catarrhalis, klebsiella pneumoniae, staphylococcus aureus, and enterococcus faecalis with elevated mics. s. pneumoniae and s. pyoge ...	2011	21343467
diarrheagenic escherichia coli in acute gastroenteritis in infants in north-west italy.	enteropathogenic escherichia coli may cause diarrhoea in infancy, but it is not routinely detected and regarded as a major causative agent. the aim of the present study was to estimate the incidence of enteropathogenic e. coli infection and to investigate its epidemiology and pathogenesis from faecal specimens in infants hospitalized for acute gastroenteritis. between march 2008 and june 2009, faecal samples were collected and examined to recognize diarrhoeal aetiology, especially for e. coli, b ...	2011	21344146
metabolism of select amino acids in bacteria from the pig small intestine.	this study investigated the metabolism of select amino acids (aa) in bacterial strains (streptococcus sp., escherichia coli and klebsiella sp.) and mixed bacterial cultures derived from the jejunum and ileum of pigs. cells were incubated at 37°c for 3 h in anaerobic media containing 0.5-5 mm select aa plus [u-(14)c]-labeled tracers to determine their decarboxylation and incorporation into bacterial protein. results showed that all types of bacteria rapidly utilized glutamine, lysine, arginine an ...	2011	21344175
synthesis and antibacterial evaluation of novel 8-fluoro norfloxacin derivatives as potential probes for methicillin and vancomycin-resistant staphylococcus aureus.	a series of novel 8-fluoro norfloxacin derivatives and the hybrids of its piperazinyl derivatives incorporated with 1,3,5-triazine and pyrimidine were synthesized. all the above compounds were evaluated for their antibacterial activity against klebsiella pneumoniae, methicillin-resistant staphylococcus aureus and methicillin & vancomycin-resistant s. aureus. among all, compounds having morpholine, n-methyl/phenyl/benzyl/pyrimidinyl piperazines and n-butylamine substitution at c-7 position, have ...	2011	21345548
microbiology and antibiotic susceptibility of organisms in bile cultures from patients with and without cholangitis at an asian academic medical center.	to review the epidemiology of microbial isolates from bile cultures taken from patients with and without cholangitis admitted to an asian academic medical center in order to compare antimicrobial sensitivities and to make recommendations for empiric antimicrobial therapy of patients with cholangitis in the philippines.	2011	21348769
phylogenetic groups among klebsiella pneumoniae isolates from brazil: relationship with antimicrobial resistance and origin.	the objectives of this study were to determine the distribution of phylogenetic groups among klebsiella pneumoniae isolates from recife, brazil and to assess the relationship between the groups and the isolation sites and resistance profile. ninety four isolates of k. pneumoniae from hospital or community infections and from normal microbiota were analyzed by gyra pcr-rflp, antibiotic susceptibility, and adonitol fermentation. the results revealed the distinction of three phylogenetic groups, as ...	2011	21350801
real-time pcr assay and a synthetic positive control for the rapid and sensitive detection of the emerging resistance gene new delhi metallo-ß-lactamase-1 (bla(ndm-1)).	carbapenems are important last-line antibiotics for the treatment of hospital infections. enterobacteriaceae (such as klebsiella pneumoniae or escherichia coli) expressing the "new delhi metallo-ß-lactamase" gene bla(ndm-1) are resistant to carbapenems and were predicted to become a major global health problem. to cope with this emerging threat, there is a need for rapid and sensitive molecular assays to detect bla(ndm-1) in carbapenem-resistant enterobacteriaceae from clinical isolates. in diag ...	2011	21350860
prevalence and characteristics of ertapenem-resistant klebsiella pneumoniae isolates in a taiwanese university hospital.	this study was conducted to investigate the prevalence and characteristics of ertapenem-resistant (etp-r) klebsiella pneumoniae isolates at a taiwanese hospital. the disk-diffusion tests revealed that the rate of ertapenem resistance among all isolates collected in 2008 was 13.5%, and the resistance rate among bloodstream isolates increased from 0% to 13.6% between 2001 and 2008. eighty-two nonduplicate etp-r isolates collected in 2008 were examined. seventy-four (90.2%) isolates of them had ext ...	2011	21352075
broad-spectrum antimicrobial efficacy of peptide a3-apo in mouse models of multidrug-resistant wound and lung infections cannot be explained by in vitro activity against the pathogens involved.	although the designer proline-rich antimicrobial peptide a3-apo has only modest activity against escherichia coli and acinetobacter baumannii in vitro, in mouse models of systemic and wound infections it shows superior efficacy compared with conventional antibiotics. in this study, the efficacy of a3-apo in several additional mouse models was investigated, including staphylococcus aureus wound infection, mixed klebsiella pneumoniae-a. baumannii-proteus mirabilis wound infection and k. pneumoniae ...	2011	21353493
prevalence of antimicrobial resistant pathogens from blood cultures from canadian hospitals: results of the canward 2007-2009 study.	this study assessed the epidemiology and antimicrobial resistance of pathogens associated with bloodstream infections in canadian hospitals between 2007 and 2009. tertiary-care medical centers representing 8 of 10 canadian provinces submitted bloodstream infection pathogens from patients attending hospital clinics, emergency rooms, medical/surgical wards, and intensive care units. over 8,000 blood culture pathogens were collected. the 10 most common pathogens (representing 80.9% of all isolates) ...	2011	21353958
in vitro activity of ceftobiprole against frequently encountered aerobic and facultative gram-positive and gram-negative bacterial pathogens: results of the canward 2007-2009 study.	the in vitro activity of ceftobiprole was evaluated against 15 011 clinical isolates obtained from patients in canadian hospitals between 2007 and 2009. all staphylococcus aureus were susceptible to ceftobiprole (mic(90)'s for methicillin-susceptible staphylococcus aureus and methicillin-resistant staphylococcus aureus of = 1 µg/ml and 2 µg/ml, respectively). ceftobiprole was active against penicillin-susceptible streptococcus pneumoniae (mic(90), = 0.06 µg/ml), penicillin-resistant streptococcu ...	2011	21353964
spontaneous eradication of a ndm-1 positive klebsiella pneumoniae that colonized the intestine of an asymptomatic carrier.		2011	21354091
activity of nxl104 in combination with beta-lactams against genetically characterized escherichia coli and klebsiella pneumoniae isolates producing class a extended-spectrum beta-lactamases and class c beta-lactamases.	the novel non-ß-lactam ß-lactamase inhibitor nxl104, in combination with cefepime, ceftazidime, ceftriaxone, amdinocillin, and meropenem, was tested against 190 extended-spectrum ß-lactamase (esbl)-producing escherichia coli and klebsiella pneumoniae isolates, 94 ampc-hyperproducing e. coli isolates, and 8 ampc/esbl-coexpressing e. coli isolates. nxl104 restored 100% susceptibility to the partner cephalosporins for all isolates tested. amdinocillin and meropenem mics were modestly improved (2 to ...	2011	21357295
transcriptional regulation of the gene cluster encoding allantoinase and guanine deaminase in klebsiella pneumoniae.	purines can be used as the sole source of nitrogen by several strains of k. pneumoniae under aerobic conditions. the genes responsible for the assimilation of purine nitrogens are distributed in three separated clusters in the k. pneumoniae genome. here, we characterize the cluster encompassing genes kpn_01787 to kpn_01791, which is involved in the conversion of allantoin into allantoate and in the deamination of guanine to xanthine. these genes are organized in three transcriptional units, hpxs ...	2011	21357483
confirmation of hiv-like sequences in respiratory tract bacteria of cambodian and kenyan hiv-positive pediatric patients.	bacteria and yeasts isolated from respiratory tracts of 39 cambodian and 28 kenyan hiv-positive children were tested for the presence of hiv-1 sequences.	2011	21358602
prevalence of fecal carriage of extended-spectrum ß-lactamase-producing enterobacteriaceae among healthy adult people in japan.	in japan, the prevalence of extended-spectrum ß-lactamase (esbl) producers in hospital settings has shown an increasing trend. in the present study, we investigated the prevalence of the fecal carriage of esbl-producing enterobacteriaceae among healthy adults. stool samples were collected for the phenotypic and genotypic identification of esbl-producing enterobacteriaceae. we found the prevalence of esbl producers to be 6.4% by phenotypic identification, and 92.9% of them possessed the bla (ctx- ...	2011	21359543
prevalence of the bla (shv) gene in klebsiella pneumoniae isolates obtained from hospital and community infections and from the microbiota of healthy individuals in recife, brazil.	the aim of this study was to determine the prevalence of the bla (shv) gene in klebsiella pneumoniae isolates from hospital and community infections and from the normal microbiota of healthy individuals in recife, pe, brazil. fifty-two k. pneumoniae isolates were analyzed regarding the presence of the bla (shv) gene, using pcr, and eight isolates were analyzed by dna sequencing. this gene was detected in 16 isolates from hospital infections, four from community infections, and nine from the norm ...	2011	21359845
[prognostic impact of extended-spectrum beta-lactamases (esbl)].		2011	21367407
foodborne nosocomial outbreak of shv1 and ctx-m-15-producing klebsiella pneumoniae: epidemiology and control.	we describe a foodborne nosocomial outbreak due to extended-spectrum ß-lactamase (esbl)-producing klebsiella pneumoniae.	2011	21367727
comparison of polymyxin b, tigecycline, cefepime, and meropenem mics for kpc-producing klebsiella pneumoniae by broth microdilution, vitek 2, and etest.	we report mic agreement and error rates between broth microdilution (bmd), vitek 2, and etest against 48 clinical kpc-producing klebsiella pneumoniae isolates for polymyxin b, tigecycline, cefepime, and meropenem. both commercial testing methods were useful for tigecycline testing; etest provided a conservative estimate of polymyxin b susceptibility. we suggest that laboratories consider the supplemental use of reference bmd or etest for cefepime and meropenem for susceptibility testing of kpc-p ...	2011	21367993
clinical and microbiological evaluation of hemodialysis-associated pneumonia (hdap): should hdap be included in healthcare-associated pneumonia?	although hemodialysis-associated pneumonia (hdap) was included among the healthcare-associated pneumonias (hcap) in the 2005 american thoracic society (ats)/infectious diseases society of america (idsa) guideline, little information relevant to clinical epidemiology, especially microbiological characteristics, is available. this study aimed to reveal microbiological characteristics and clinical outcomes of hdap and to assess whether hdap should be included in the hcap category. we retrospectivel ...	2011	21369775
rapid increase of carbapenemase-producing klebsiella pneumoniae strains in a large italian hospital: surveillance period 1 march - 30 september 2010.	the first case of carbapenemase-producing enterobacteriaceae in italy was reported in 2009. we performed a study over a period of seven months in 2010 to survey the circulation of klebsiella pneumoniae carbapenemases (kpc) ina 1,500-bed university hospital in northern italy and report the presence and rapid increase of these multidrug-resistant bacteria. the results raise a major concern about these pathogens and demonstrate the urgent need for infection control and antibiotic stewardship progra ...	2011	21371414
[mortality risk factors for bloodstream infections caused by extended-spectrum beta-lactamase-producing microorganisms].	to identify risk factors for mortality in patients with bloodstream infection by extended-spectrum beta-lactamase (esbl)-producing microorganisms.	2011	21371702
microbiological profile of haematogenous osteoarticular infections in children.	introduction: staphylococcus aureus, kingella kingae and ß-hemolytic streptococcus are presently the most frequently identified bacteria in child haematogenous osteoarticular infection. objective: to determine the microbiological profile (bacteria and antibiotic susceptibility) of osteoarticular infections in a paediatric hospital, so as to adapt treatment protocols to the ecology of the bacteria isolated. patients and methods: prospective descriptive study, including children admitted for acute ...	2011	21371961
evaluation of a commercial multiplex pcr test (septifast) in the etiological diagnosis of community-onset bloodstream infections.	the commercial polymerase chain reaction (pcr) test, septifast, is designed to identify the dna of individual bacterial and fungal pathogens in whole blood. we aimed to evaluate the usefulness of the test for the detection of community-onset bloodstream infections. we prospectively included adult patients who were subjected to blood culture (bc) at an infectious diseases department. for the evaluation, one bc/pcr set (two bc bottles and one pcr tube) per patient was used. when several sets were ...	2011	21373774
facultative methylotrophs from the human oral cavity and methylotrophy in strains of gordonia, leifsonia, and microbacterium.	we show that bacteria with methylotrophic potential are ubiquitous in the human mouth microbiota. numerous strains of actinobacteria (brevibacterium, gordonia, leifsonia, microbacterium, micrococcus, rhodococcus) and proteobacteria (achromobacter, klebsiella, methylobacterium, pseudomonas, ralstonia) were isolated, and one strain of each of the eleven genera was studied in detail. these strains expressed enzymes associated with methylotrophic metabolism (methanol, methylamine, and formate dehydr ...	2011	21374057
epidemiology and clinical features of community-onset bacteremia caused by extended-spectrum +¦-lactamase-producing klebsiella pneumoniae.	there is limited clinical information regarding community-onset bacteremia caused by extended-spectrum +¦-lactamase (esbl)-producing klebsiella pneumoniae. this study was performed to evaluate risk factors and clinical outcomes of community-onset bacteremia caused by esbl-producing k. pneumoniae. a total of 435 patients with community-onset k. pneumoniae bacteremia were included and data from patients with esbl-producing k. pneumoniae bacteremia were compared to those with non-esbl-producing bac ...	2011	21388296
common pathogens in burn wound and changes in their drug sensitivity.	infection is an important cause of mortality in patients with burns. rapid emergence of hospital pathogens and antibiotic-resistant organisms necessitate periodic evaluation of bacterial colonisation patterns and antibiogram sensitivity in burn wards. in this study, which was conducted in a 3 months' period in 2009, 106 samples from the wounds of 59 patients admitted in a burn ward were taken, one in the 1st and one between the 3rd and the 7th days. pseudomonas aeruginosa, acinetobacter and kleb ...	2011	21388742
cytotoxicity, antiviral and antimicrobial activities of alkaloids, flavonoids, and phenolic acids.	some natural products consisting of the alkaloids yohimbine and vincamine (indole-type), scopolamine and atropine (tropane-type), colchicine (tropolone-type), allantoin (imidazolidine-type), trigonelline (pyridine-type) as well as octopamine, synephrine, and capsaicin (exocyclic amine-type); the flavonoid derivatives quercetin, apigenin, genistein, naringin, silymarin, and silibinin; and the phenolic acids namely gallic acid, caffeic acid, chlorogenic acid, and quinic acid, were tested for their ...	2011	21391841
urinary tract infections owing to esbl-producing bacteria: microorganisms change--clinical pattern does not.	urinary tract infections (utis) caused by extended-spectrum beta lactamase (esbl)-producing bacteria have become a growing problem worldwide. the aim of this study was to investigate the clinical and radiological findings in patients with community-acquired utis owing to esbl-producing bacteria.	2011	21392102
[endocarditis caused by extended spectrum beta lactamase producing klebsiella].		2011	21392740
transfer of oxa-48-positive carbapenem-resistant klebsiella pneumoniae from turkey to france.		2011	21393135
sepsis in neonates due to imipenem-resistant klebsiella pneumoniae producing ndm-1 in india.		2011	21393155
radical reduction of cephalosporin use at a tertiary hospital after educational antibiotic intervention during an outbreak of extended-spectrum beta-lactamase-producing klebsiella pneumoniae.	during an outbreak of extended-spectrum +¦-lactamase (esbl)-producing klebsiella pneumoniae at our hospital, we performed an educational antibiotic intervention aimed at reducing prescriptions of second- and third-generation cephalosporins and preventing increased use of fluoroquinolones and carbapenems. in this report, we describe the implementation strategy used and evaluate the intervention effect according to cochrane recommendations.	2011	21393158
a 12 year (1998-2009) antibiotic resistance surveillance of klebsiella pneumoniae collected from intensive care and urology patients in 14 dutch hospitals.	we evaluated the changes in antibiotic resistance from 1998 to 2009 of klebsiella pneumoniae isolated from the intensive care units (icus) and urology services of 14 dutch hospitals and the consequences for empirical therapy.	2011	21393165
does broad-spectrum beta-lactam resistance due to ndm-1 herald the end of the antibiotic era for treatment of infections caused by gram-negative bacteria?	the ndm-1 gene, first identified in sweden in 2008 in klebsiella pneumoniae from a patient hospitalized in new delhi, encodes a metallo-+¦-lactamase that inactivates all +¦-lactams except aztreonam. this bla(ndm-1) gene has been identified in hospital-acquired bacterial species, such as k. pneumoniae, but also in the typical community-acquired species, escherichia coli. this gene has been identified in strains that possess other resistance mechanisms contributing to their multidrug resistance pa ...	2011	21393184
arrival of klebsiella pneumoniae carbapenemase (kpc)-2 in taiwan.		2011	21393195
colistin heteroresistance in carbapenemase-producing klebsiella pneumoniae.		2011	21393203
small qnrb-harbouring cole-like plasmids widespread in commensal enterobacteria from a remote amazonas population not exposed to antibiotics.		2011	21393218