Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| multisite evaluation of cepheid xpert carba-r assay for detection of carbapenemase-producing organisms in rectal swabs. | rapid identification of patients who are colonized with carbapenemase-producing organisms (cpo) is included in multiple national guidelines for containment of these organisms. in a multisite study, we evaluated the performance of the cepheid xpert carba-r assay, a qualitative diagnostic test that was designed for the rapid detection and differentiation of the blakpc, blandm, blavim, blaoxa-48, and blaimp-1 genes from rectal swab specimens. a double rectal swab set was collected from 383 patients ... | 2016 | 27122379 |
| use of attenuated but metabolically competent salmonella as a probiotic to prevent or treat salmonella infection. | salmonella enterica is among the most burdensome of foodborne disease agents. there are over 2,600 serovars that cause a range of disease manifestations ranging from enterocolitis to typhoid fever. while there are two vaccines in use in humans to protect against typhoid fever, there are none that prevent enterocolitis. if vaccines preventing enterocolitis were to be developed, they would likely protect against only one or a few serovars. in this report, we tested the hypothesis that probiotic or ... | 2016 | 27185789 |
| performance evaluation of three automated identification systems in detecting carbapenem-resistant enterobacteriaceae. | carbapenem-resistant enterobacteriaceae (cre) is prevalent around the world. rapid and accurate detection of cre is urgently needed to provide effective treatment. automated identification systems have been widely used in clinical microbiology laboratories for rapid and high-efficient identification of pathogenic bacteria. however, critical evaluation and comparison are needed to determine the specificity and accuracy of different systems. the aim of this study was to evaluate the performance of ... | 2016 | 27328762 |
| detection of carbapenemase genes in aquatic environments in rio de janeiro, brazil. | this study reveals the presence of different carbapenemase genes (blakpc, blandm, blages, and blaoxa48-like genes) detected directly from water samples and clonal dispersion (by pulsed-field gel electrophoresis [pfge] and multilocus sequence typing [mlst]) of kpc-2-producing enterobacteriaceae in two important urban aquatic matrixes from rio de janeiro, brazil, highlighting the role of aquatic environments as gene pools and the possibility of community spreading. | 2016 | 27139469 |
| molecular diversity and plasmid analysis of kpc-producing escherichia coli. | the emergence and spread of klebsiella pneumoniae carbapenemase (kpc) among enterobacteriaceae presents a major public health threat to the world. although not as common as in k. pneumoniae, kpc is also found in escherichia coli strains. here, we genetically characterized 9 carbapenem-resistant e. coli strains isolated from six hospitals in the united states and completely sequenced their blakpc-harboring plasmids. the nine strains were isolated from different geographical locations and belonged ... | 2016 | 27114279 |
| mechanisms involved in acquisition of blandm genes by inca/c2 and incfiiy plasmids. | blandm genes confer carbapenem resistance and have been identified on transferable plasmids belonging to different incompatibility (inc) groups. here we present the complete sequences of four plasmids carrying a blandm gene, pkp1-ndm-1, pec2-ndm-3, pecl3-ndm-1, and pec4-ndm-6, from four clinical samples originating from four different patients. different plasmids carry segments that align to different parts of the blandm region found on acinetobacter plasmids. pkp1-ndm-1 and pec2-ndm-3, from kle ... | 2016 | 27114281 |
| plant growth promoting rhizobacteria and silicon synergistically enhance salinity tolerance of mung bean. | the present study explored the eco-friendly approach of utilizing plant-growth-promoting rhizobacteria (pgpr) inoculation and foliar application of silicon (si) to improve the physiology, growth, and yield of mung bean under saline conditions. we isolated 18 promising pgpr from natural saline soil in saudi arabia, and screened them for plant-growth-promoting activities. two effective strains were selected from the screening trial, and were identified as enterobacter cloacae and bacillus drentens ... | 2016 | 27379151 |
| vgrg c terminus confers the type vi effector transport specificity and is required for binding with paar and adaptor-effector complex. | type vi secretion system (t6ss) is a macromolecular machine used by many gram-negative bacteria to inject effectors/toxins into eukaryotic hosts or prokaryotic competitors for survival and fitness. to date, our knowledge of the molecular determinants and mechanisms underlying the transport of these effectors remains limited. here, we report that two t6ss encoded valine-glycine repeat protein g (vgrg) paralogs in agrobacterium tumefaciens c58 specifically control the secretion and interbacterial ... | 2016 | 27313214 |
| a broad-spectrum infection diagnostic that detects pathogen-associated molecular patterns (pamps) in whole blood. | blood cultures, and molecular diagnostic tests that directly detect pathogen dna in blood, fail to detect bloodstream infections in most infected patients. thus, there is a need for a rapid test that can diagnose the presence of infection to triage patients, guide therapy, and decrease the incidence of sepsis. | 2016 | 27333027 |
| causality of small and large intestinal microbiota in weight regulation and insulin resistance. | the twin pandemics of obesity and type 2 diabetes (t2d) are a global challenge for health care systems. changes in the environment, behavior, diet, and lifestyle during the last decades are considered the major causes. a western diet, which is rich in saturated fat and simple sugars, may lead to changes in gut microbial composition and physiology, which have recently been linked to the development of metabolic diseases. | 2016 | 27617199 |
| in vitro activity of oral antimicrobial agents against pathogens associated with community-acquired upper respiratory tract and urinary tract infections: a five country surveillance study. | bacterial infections that cause community-acquired urinary tract infections (ca-uti) and upper respiratory tract infections (ca-urti) are most frequently treated empirically. however, an increase in antimicrobial resistance has become a problem when treating outpatients. | 2016 | 27287766 |
| neonatal infections with multidrug-resistant esbl-producing e. cloacae and k. pneumoniae in neonatal units of two different hospitals in antananarivo, madagascar. | we investigated the molecular mechanism of ß-lactam resistance in extended-spectrum ß-lactamase (esbl)-producing enterobacterial strains isolated in neonatal units of different hospitals in anatnanarivo, madagascar. | 2016 | 27287441 |
| complete genome sequence of a clinical isolate of enterobacter asburiae. | we report here the complete genome sequence of enterobacter asburiae strain enipbj-cg1, isolated from a bone marrow transplant patient. the size of the genome sequence is approximately 4.65 mb, with a g+c content of 55.76%, and it is predicted to contain 4,790 protein-coding genes. | 2016 | 27284137 |
| efficient production of acetoin in saccharomyces cerevisiae by disruption of 2,3-butanediol dehydrogenase and expression of nadh oxidase. | acetoin is widely used in food and cosmetic industry as taste and fragrance enhancer. for acetoin production in this study, saccharomyces cerevisiae jhy605 was used as a host strain, where the production of ethanol and glycerol was largely eliminated by deleting five alcohol dehydrogenase genes (adh1, adh2, adh3, adh4, and adh5) and two glycerol 3-phosphate dehydrogenase genes (gpd1 and gpd2). to improve acetoin production, acetoin biosynthetic genes from bacillus subtilis encoding α-acetolactat ... | 2016 | 27279026 |
| antimicrobial activity of serbian propolis evaluated by means of mic, hptlc, bioautography and chemometrics. | new information has come to light about the biological activity of propolis and the quality of natural products which requires a rapid and reliable assessment method such as high performance thin-layer chromatography (hptlc) fingerprinting. this study investigates chromatographic and chemometric approaches for determining the antimicrobial activity of propolis of serbian origin against various bacterial species. a linear multivariate calibration technique, using partial least squares, was used t ... | 2016 | 27272728 |
| cockroaches as a source of high bacterial pathogens with multidrug resistant strains in gondar town, ethiopia. | background. cockroaches are source of bacterial infections and this study was aimed to assess bacterial isolates and their antimicrobial profiles from cockroaches in gondar town, ethiopia. methods. a total of 60 cockroaches were collected from march 1 to may 30, 2014, in gondar town. bacterial species were isolated from external and internal parts of cockroaches. disk diffusion method was used to determine antibiotic susceptibility patterns. data were entered and analyzed by using spss version 2 ... | 2016 | 27340653 |
| complete genome sequence of enterobacter cloacae b2-dha, a chromium-resistant bacterium. | previously, we reported a chromium-resistant bacterium, enterobacter cloacae b2-dha, isolated from the landfills of tannery industries in bangladesh. here, we investigated its genetic composition using massively parallel sequencing and comparative analysis with other known enterobacter genomes. assembly of the sequencing reads revealed a genome of ~4.21 mb in size. | 2016 | 27257201 |
| emergence of cmy-2- and dha-1-type ampc β-lactamases in enterobacter cloacae isolated from several hospitals of qazvin and tehran, iran. | the emergence of plasmid-mediated ampc (pampc) β-lactamases conferring resistance to third-generation cephalosporins has become a major clinical concern worldwide. the aims of this study were to determine the prevalence of pampc-producing e. cloacae isolates and typing of them in qazvin and tehran provinces, iran. | 2016 | 27928483 |
| antimicrobial effect of ozone made by kp syringe of high-frequency ozone generator. | the aim of this study was to evaluate in vitro the antibacterial effect of ozone on suspension of three different bacteria inoculated in prepared canals of extracted human teeth. | 2016 | 27789911 |
| febrile neutropenia in children with acute lymphoblastic leukemia: single center experience. | an important life-threatening complication of intensive chemotherapy administered in children with leukemia is febrile neutropenia. the objective of this study was to evaluate the clinical features and consequences of febrile neutropenia attacks in children who were treated for acute lymphoblastic leukemia. | 2016 | 27489464 |
| muscles provide protection during microbial infection by activating innate immune response pathways in drosophila and zebrafish. | muscle contraction brings about movement and locomotion in animals. however, muscles have also been implicated in several atypical physiological processes including immune response. the role of muscles in immunity and the mechanism involved has not yet been deciphered. in this paper, using drosophila indirect flight muscles (ifms) as a model, we show that muscles are immune-responsive tissues. flies with defective ifms are incapable of mounting a potent humoral immune response. upon immune chall ... | 2016 | 27101844 |
| transcriptional regulation of the outer membrane porin gene ompw reveals its physiological role during the transition from the aerobic to the anaerobic lifestyle of escherichia coli. | understanding bacterial physiology relies on elucidating the regulatory mechanisms and cellular functions of those differentially expressed genes in response to environmental changes. a widespread gram-negative bacterial outer membrane protein ompw has been implicated in the adaptation to stresses in various species. it is recently found to be present in the regulon of the global anaerobic transcription factor fnr and arca in escherichia coli. however, little is known about the physiological imp ... | 2016 | 27303386 |
| clinical and corneal microbial profile of infectious keratitis in a high hiv prevalence setting in rural south africa. | the purpose of this investigation was to determine the clinical and corneal microbial profile of infectious keratitis in a high human immunodeficiency virus (hiv) prevalence setting in rural south africa. data in this cross-sectional study were collected from patients presenting with symptoms of infectious keratitis (n = 46) at the ophthalmology outpatient department of three hospitals in rural south africa. corneal swabs were tested for herpes simplex virus type 1 (hsv-1) and 2 (hsv-2), varicel ... | 2016 | 27236644 |
| analysis of cultivable microbiota and diet intake pattern of the long-lived naked mole-rat. | a variety of microbial communities exist throughout the human and animal body. genetics, environmental factors and long-term dietary habit contribute to shaping the composition of the gut microbiota. for this reason the study of the gut microbiota of a mammal exhibiting an extraordinary life span is of great importance. the naked mole-rat (heterocephalus glaber) is a eusocial mammal known for its longevity and cancer resistance. | 2016 | 27239229 |
| serratia fonticola, pathogen or bystander? a case series and review of the literature. | serratia fonticola is an unusual human pathogen, previously described primarily as causing skin and soft tissue infections following trauma. there is little information in the literature about its treatment or susceptibilities. we describe the case of a 67-year-old male with paraplegia who developed urosepsis due to s. fonticola. blood and urine cultures obtained prior to the initiation of antimicrobials both grew s. fonticola. the patient completed a 15-day course of antimicrobials and had an u ... | 2016 | 27347484 |
| amino acid substitutions of crrb responsible for resistance to colistin through crrc in klebsiella pneumoniae. | colistin is a last-resort antibiotic for treatment of carbapenem-resistant klebsiella pneumoniae a recent study indicated that missense mutations in the crrb protein contribute to colistin resistance. in our previous study, mechanisms of colistin resistance were defined in 17 of 26 colistin-resistant k. pneumoniae clinical isolates. of the remaining nine strains, eight were highly resistant to colistin. in the present study, crrab sequences were determined for these eight strains. six separate a ... | 2016 | 27067316 |
| impact of cefepime susceptible-dose-dependent mic for enterobacteriaceae on reporting and prescribing. | the clsi cefepime breakpoints were revised to include a susceptible-dose-dependent (sdd) category for enterobacteriaceae, with the intention that higher cefepime doses be used for these isolates. in this study, 1.6% of enterobacteriaceae isolates were reported as sdd, with escherichia coli the most common sdd organism. cefepime was prescribed in three cases (4.8%); the majority of sdd isolates were treated with a carbapenem. enterobacteriaceae with cefepime sdd mics were not commonly treated wit ... | 2016 | 27067319 |
| rapid flow cytometry test for identification of different carbapenemases in enterobacteriaceae. | a flow cytometry test was developed to identify carbapenemase production by enterobacteriaceae and to discriminate between the different types of carbapenemases (classes a, b, and d). it is based on the detection of meropenem activity against bacteria, coupled with different carbapenemase inhibitors, which is assessed by flow cytometry. it represents a convenient, fast, and reliable approach (100% sensitivity and 100% specificity) for the detection and characterization of different carbapenemase ... | 2016 | 27021318 |
| emergence of the plasmid-mediated mcr-1 gene in colistin-resistant enterobacter aerogenes and enterobacter cloacae. | 2016 | 26976876 | |
| evaluation of multiple methods for detection of gastrointestinal colonization of carbapenem-resistant organisms from rectal swabs. | rectal swabs from high-risk patients were screened for carbapenem-resistant organisms (cros) using several methods. the direct macconkey plate method was the most sensitive for cros (95%), while chromid carba and the check-direct cpe screen assay were the most sensitive for the detection of carbapenemase-producing organisms (cpos) (100%; all blakpc). all methods had a specificity of >90% for cros, and for cpos, the specificity ranged from 85 to 98%. broth enrichment methods performed poorly comp ... | 2016 | 27053674 |
| high intravascular tissue factor-but not extracellular microvesicles-in septic patients is associated with a high saps ii score. | sepsis is associated with coagulation abnormalities, and a high content of intravascular tissue factor (tf) may contribute to the development of multisystem organ failure. circulating microvesicles (mvs) are increased during sepsis and characterized by their phosphatidylserine content. it is unclear whether mvs-as a part of the host response to the infection-are beneficial or rather contribute to systemic complications in sepsis. in the present prospective clinical pilot study, we investigated w ... | 2016 | 27217958 |
| a novel multi-epitope recombined protein for diagnosis of human brucellosis. | in epidemic regions of the world, brucellosis is a reemerging zoonosis with minimal mortality but is a serious public hygiene problem. currently, there are various methods for brucellosis diagnosis, however few of them are available to be used to diagnose, especially for serious cross-reaction with other bacteria. | 2016 | 27206475 |
| bacterial cholangitis, cholecystitis, or both in dogs. | bacterial cholangitis and cholecystitis are rarely reported, poorly characterized diseases in the dog. | 2016 | 27203848 |
| extended v-y advancement flap reconstruction of a large posterior upper midline trunk defect. | large posterior upper trunk defects can be challenging to reconstruct. trapezius or latissimus dorsi myocutaneous flaps are principally utilized for reconstruction; however, some defects may not be amenable to this standard approach. here, we describe a patient with a full-thickness skin and subcutaneous tissue loss of the upper back and inferior cervical region after dermatofibrosarcoma protuberans resection. a large, extended v-y flap was used for closure of this wound secondary to its locatio ... | 2016 | 27579237 |
| ultrasound guided placement of the distal catheter in paediatric ventriculoatrial shunts-an appraisal of efficacy and complications. | ventriculoatrial (va) shunts are commonly used as a second-line treatment of hydrocephalus when the peritoneum is an unsuitable site for the distal catheter. many centres now utilise ultrasound and interventional radiology techniques to aid placement of the distal catheter. the purpose of this study was to conduct a contemporary audit of va shunting in children using interventional radiology techniques for placement of the distal catheter. | 2016 | 27207611 |
| fine needle aspiration in the investigation of thyroid nodules. | thyroid nodules are a common finding in germany. most are benign; thyroid cancer is very rare. the challenge for the physician is to diagnose malignant tumors early. fine needle aspiration is an important tool for this purpose. | 2016 | 27294815 |
| saccharomyces cerevisiae osteomyelitis in an immunocompetent baker. | invasive infection caused by saccharomyces cerevisiae is rare. we report the first case of osteomyelitis caused by s. cerevisiae (baker's yeast) in a post-traumatic patient. the clinical outcome was favorable after surgical debridement, prolonged antifungal treatment and hyperbaric oxygen therapy. | 2016 | 27347482 |
| correlating cleaning thoroughness with effectiveness and briefly intervening to affect cleaning outcomes: how clean is cleaned? | the most efficient approach to monitoring and improving cleaning outcomes remains unresolved. we sought to extend the findings of a previous study by determining whether cleaning thoroughness (dye removal) correlates with cleaning efficacy (absence of molecular or cultivable biomaterial) and whether one brief educational intervention improves cleaning outcomes. | 2016 | 27196635 |
| colonization of multidrug resistant pathogens in a hybrid pediatric cardiac surgery center. | the incidence of multidrug resistant microorganisms worldwide is increasing. the aim of the study was to present institutional experience with the multidrug resistant microorganism colonization patterns observed in children with congenital heart diseases hospitalized in a hybrid pediatric cardiac surgery center. | 2016 | 27279859 |
| decreasing level of resistance in invasive klebsiella pneumoniae strains isolated in marseille, january 2012-july 2015. | klebsiella pneumoniae is a gram-negative bacterial species well known for its capacity to cause infections in humans, and to carry and spread a wide variety of resistance genes including extended-spectrum beta-lactamase genes, carbapenem resistance genes, and colistin resistance genes. recently, our real-time laboratory-based surveillance system marss (the marseille antibiotic resistance surveillance system) allowed us to observe a intringing dramatic decrease in the beta-lactam resistance level ... | 2016 | 27330897 |
| antimicrobial activity of tulsi (ocimum tenuiflorum) essential oil and their major constituents against three species of bacteria. | in recent years scientists worldwide have realized that the effective life span of any antimicrobial agent is limited, due to increasing development of resistance by microorganisms. consequently, numerous studies have been conducted to find new alternative sources of antimicrobial agents, especially from plants. the aims of this project were to examine the antimicrobial properties of essential oils distilled from australian-grown ocimum tenuiflorum (tulsi), to quantify the volatile components pr ... | 2016 | 27242708 |
| draft genome sequence of hydrocarbon-degrading enterobacter cloacae strain s1:cnd1, isolated from crude oil-contaminated soil from the noonmati oil refinery, guwahati, assam, india. | we report here the 4.57-mb draft genome sequence of hydrocarbon-degrading enterobacter cloacae strain s1:cnd1 isolated from oil-contaminated soil in guwahati, india. s1:cnd1 contains 4,205 coding sequences and has a g+c content of 57.45%. this is the first report of the genome sequence of an e. cloacae adapted to an oil-contaminated environment. | 2016 | 27174279 |
| kinetic characterization of ges-22 β-lactamase harboring the m169l clinical mutation. | the class a β-lactamase ges-22 has been identified in acinetobacter baumannii isolates in turkey, and subsequently shown to differ from ges-11 by a single substitution (m169l). because m169 is part of the omega loop, a structure that is known to have major effects on substrate selectivity in class a β-lactamases, we expressed, purified and kinetically characterized this novel variant. our results show that compared with ges-11(6 × his), ges-22(6 × his) displays more efficient hydrolysis of penic ... | 2016 | 27168312 |
| metallo-β-lactamase (mbl)-producing enterobacteriaceae in united states children. | metallo-β-lactamases (mbls) are emerging as the most notable resistance determinants in enterobacteriaceae. in many cases, the genes encoding mbls are part of complex, mobile genetic elements that carry other resistance determinants. in the united states, there are increasing reports of mbl-producing enterobacteriaceae, with new delhi mbls (ndms) accounting for the majority of transmissible mbl infections. many infections caused by ndm-producing bacteria are associated with international travel ... | 2016 | 27419164 |
| [factors associated with death in newborns suspected of bacterial infections in pediatric teaching hospital of charles de gaulle in ouagadougou, burkina faso]. | 2016 | 27583071 | |
| antibacterial and antibiotic-modulation activity of six cameroonian medicinal plants against gram-negative multi-drug resistant phenotypes. | bacterial infections involving multi-drug resistant (mdr) phenotypes constitute a worldwide health concern. the present work was designed to assess the antibacterial properties of the methanol extracts of six medicinal plants (anthocleista schweinfurthii, nauclea latifolia, boehmeria platyphylla, caucalis melanantha, erigeron floribundus and zehneria scobra) and the effects of their associations with antibiotics on mdr gram-negative bacteria over-expressing active efflux pumps. | 2016 | 27142515 |
| co-spread of metal and antibiotic resistance within st3-inchi2 plasmids from e. coli isolates of food-producing animals. | concerns have been raised in recent years regarding co-selection for antibiotic resistance among bacteria exposed to heavy metals, particularly copper and zinc, used as growth promoters for some livestock species. in this study, 25 inchi2 plasmids harboring oqxab (20/25)/blactx-m (18/25) were found with sizes ranging from ∼260 to ∼350 kb and 22 belonged to the st3-inchi2 group. in addition to blactx-m and oqxab, pcoa-e (5/25) and sile-p (5/25), as well as aac(6')-ib-cr (18/25), flor (16/25), rmt ... | 2016 | 27143648 |
| refined spruce resin to treat chronic wounds: rebirth of an old folkloristic therapy. | significance: the treatment of chronic wounds results in an enormous drain on healthcare resources in terms of workload, costs, frustration, and impaired quality of life, and it presents a clinical challenge for physicians worldwide. effective local treatment of a chronic wound has an important role, particularly in patients who are-because of their poor general condition, diminished life expectancy, or unacceptable operative risk-outside of surgical treatment. recent advances: since 2002, our m ... | 2016 | 27134764 |
| liver abscess formation following transarterial chemoembolization: clinical features, risk factors, bacteria spectrum, and percutaneous catheter drainage. | to investigate the clinical features, risk factors, and bacterial spectrum of liver abscess following transarterial chemoembolization (tace) and evaluate the therapeutic effect of percutaneous catheter drainage (pcd) on the abscesses.a retrospective review of patient charts was performed in 3613 patients who suffered from liver malignancies (2832 patients with hepatocellular carcinoma and 781 with metastatic hepatic tumor) and had undergone 11,054 tace procedures from january 2005 to october 201 ... | 2016 | 27124055 |
| polysaccharide-capped silver nanoparticles inhibit biofilm formation and eliminate multi-drug-resistant bacteria by disrupting bacterial cytoskeleton with reduced cytotoxicity towards mammalian cells. | development of effective anti-microbial therapeutics has been hindered by the emergence of bacterial strains with multi-drug resistance and biofilm formation capabilities. in this article, we report an efficient green synthesis of silver nanoparticle (agnp) by in situ reduction and capping with a semi-synthetic polysaccharide-based biopolymer (carboxymethyl tamarind polysaccharide). the cmt-capped agnps were characterized by uv, dls, fe-sem, edx and hr-tem. these agnps have average particle size ... | 2016 | 27125749 |
| virulence characteristics of extraintestinal pathogenic escherichia coli deletion of gene encoding the outer membrane protein x. | outer membrane protein x (ompx) and its homologues have been proposed to contribute to the virulence in various bacterial species. but, their role in virulence of extraintestinal pathogenic escherichia coli (expec) is yet to be determined. this study evaluates the role of ompx in expec virulence in vitro and in vivo using a clinical strain ppecc42 of porcine origin. the ompx deletion mutant exhibited increased swimming motility and decreased adhesion to, and invasion of pulmonary epithelial a549 ... | 2016 | 27149893 |
| antibacterial effect of copper on microorganisms isolated from bovine mastitis. | the antimicrobial properties of copper have been recognized for several years; applying these properties to the prevention of diseases such as bovine mastitis is a new area of research. the aim of the present study was to evaluate in vitro the antimicrobial activity of copper on bacteria isolated from subclinical and clinical mastitis milk samples from two regions in chile. a total of 327 microorganisms were recovered between march and september 2013, with different prevalence by sample origin ( ... | 2016 | 27199953 |
| detection and genotype analysis of ampc β-lactamase in klebsiella pneumoniae from tertiary hospitals. | the aim of the present study was to investigate the phenotype and genotype of plasmid-mediated ampc (pampc) β-lactamase in klebsiella pneumoniae and its antibiotic resistance. a total of 130 non-repetitive clinical isolates of klebsiella pneumoniae, obtained from tertiary hospitals, were phenotypically screened for pampc β-lactamase production with the cefoxitin disk diffusion test. β-lactamase genes in the screened isolates were detected using multiplex polymerase chain reaction (pcr); carbapen ... | 2016 | 27347082 |
| dermatomyositis and polymyositis in the intensive care unit: a single-center retrospective cohort study of 102 patients. | patients with idiopathic inflammatory myopathies (iims) are sometimes complicated with life-threatening conditions requiring intensive care unit (icu) admission. in the past, owing to the low incidence of iim, little was known about such patients. our aim was to investigate the clinical features and outcomes of these patients and identify their risk factors for mortality. | 2016 | 27115138 |
| enterobacter cloacae inhibits human norovirus infectivity in gnotobiotic pigs. | human noroviruses (hunovs) are the leading cause of epidemic gastroenteritis worldwide. study of hunov biology has been hampered by the lack of an efficient cell culture system. recently, enteric commensal bacteria enterobacter cloacae has been recognized as a helper in hunov infection of b cells in vitro. to test the influences of e. cloacae on hunov infectivity and to determine whether hunov infects b cells in vivo, we colonized gnotobiotic pigs with e. cloacae and inoculated pigs with 2.74 × ... | 2016 | 27113278 |
| a novel family viii carboxylesterase hydrolysing third- and fourth-generation cephalosporins. | a metagenomic library was constructed from a soil sample of spindle tree-rhizosphere. from this library, one clone with esterase activity was selected. the sequence analysis revealed an open reading frame (eststr1) encoded protein of 390 amino acids. eststr1 is a family viii carboxylesterase and retains the s-x-x-k motif conserved in both family viii carboxylesterases and class c β-lactamases. the eststr1 gene was overexpressed in e. coli and the recombinant protein was purified by purified by m ... | 2016 | 27186489 |
| susceptibility of multidrug-resistant gram-negative urine isolates to oral antibiotics. | increasing resistance among gram-negative uropathogens limits treatment options, and susceptibility data for multidrug-resistant isolates are limited. we assessed the activity of five oral agents against 91 multidrug-resistant gram-negative urine isolates that were collected from emergency department/hospitalized patients. fosfomycin and nitrofurantoin were most active (>75% susceptibility). susceptibilities to sulfamethoxazole-trimethoprim, ciprofloxacin, and ampicillin were ≤40%; empirical use ... | 2016 | 26883704 |
| carbapenem- and colistin-resistant enterobacter cloacae from delta, colorado, in 2015. | resistance to carbapenems in enterobacteriaceae is a clinical problem of growing significance. difficulty in treating multidrug-resistant gram-negative organisms with conventional antibiotics has led to a renewed and increasing use of polymyxin compounds, such as colistin. here, we report the isolation of carbapenem- and colistin-resistant enterobacter cloacae from a polymicrobial lower extremity wound in an ambulatory patient. whole-genome sequencing demonstrated the presence of chromosomal bla ... | 2016 | 26883705 |
| detection of plasmid-mediated ampc β-lactamase in escherichia coli. | escherichia coli (e. coli) is a common opportunistic pathogen for nosocomial infection. the aim of the study was to examine the phenotype, genotype and epidemiology of plasmid-mediated ampc β-lactamases in e. coli. in total, 96 clinical isolates of repeated e. coli were collected from different hospitals between august and october 2012. using a cefoxitin disk diffusion method to identify the phenotype of ampc β-lactamases in e. coli, the plasmid was extracted, and multiplex polymerase chain reac ... | 2016 | 27284407 |
| new structures simultaneously harboring class 1 integron and iscr1-linked resistance genes in multidrug-resistant gram-negative bacteria. | the connection structure of class 1 integron and insertion sequence common region 1 (iscr1) is called "complex class 1 integrons" or "complex sul1-type integrons", which is also known to be associated with many resistance genes. this structure is a powerful gene-capturing tool kit that can mobilize antibiotic resistance genes. in order to look for and study the structure among clinical multidrug-resistant (mdr) gram-negative isolates, 63 isolates simultaneously harbored class 1 integron and iscr ... | 2016 | 27103443 |
| extensively drug-resistant bacteria are an independent predictive factor of mortality in 130 patients with spontaneous bacterial peritonitis or spontaneous bacteremia. | to evaluate the epidemiology and outcomes of culture-positive spontaneous bacterial peritonitis (sbp) and spontaneous bacteremia (sb) in decompensated cirrhosis. | 2016 | 27099449 |
| strain-level dissection of the contribution of the gut microbiome to human metabolic disease. | the gut microbiota has been linked with metabolic diseases in humans, but demonstration of causality remains a challenge. the gut microbiota, as a complex microbial ecosystem, consists of hundreds of individual bacterial species, each of which contains many strains with high genetic diversity. recent advances in genomic and metabolomic technologies are facilitating strain-level dissection of the contribution of the gut microbiome to metabolic diseases. interventional studies and correlation anal ... | 2016 | 27098841 |
| the sandfly lutzomyia longipalpis ll5 embryonic cell line has active toll and imd pathways and shows immune responses to bacteria, yeast and leishmania. | lutzomyia longipalpis is the main vector of visceral leishmaniasis in latin america. sandfly immune responses are poorly understood. in previous work we showed that these vector insects respond to bacterial infections by modulating a defensin gene expression and activate the imd pathway in response to leishmania infection. aspects of innate immune pathways in insects (including mosquito vectors of human diseases) have been revealed by studying insect cell lines, and we have previously demonstrat ... | 2016 | 27098567 |
| duplex dna-invading γ-modified peptide nucleic acids enable rapid identification of bloodstream infections in whole blood. | bloodstream infections are a leading cause of morbidity and mortality. early and targeted antimicrobial intervention is lifesaving, yet current diagnostic approaches fail to provide actionable information within a clinically viable time frame due to their reliance on blood culturing. here, we present a novel pathogen identification (pid) platform that features the use of duplex dna-invading γ-modified peptide nucleic acids (γpnas) for the rapid identification of bacterial and fungal pathogens di ... | 2016 | 27094328 |
| isolation and characterization of aquatic-borne klebsiella pneumoniae from tropical estuaries in malaysia. | klebsiella pneumoniae is an opportunistic pathogen that is responsible for causing nosocomial and community-acquired infections. despite its common presence in soil and aquatic environments, the virulence potential of k. pneumoniae isolates of environmental origin is largely unknown. hence, in this study, k. pneumoniae isolated from the estuarine waters and sediments of the matang mangrove estuary were screened for potential virulence characteristics: antibiotic susceptibility, morphotype on con ... | 2016 | 27092516 |
| a glimpse into evolution and dissemination of multidrug-resistant acinetobacter baumannii isolates in east asia: a comparative genomics study. | clonal dissemination is characteristic of the important nosocomial pathogen acinetobacter baumannii, as revealed by previous multi-locus sequence typing (mlst) studies. however, the disseminated phyletic unit is actually mlst sequence type instead of real bacterial clone. here we sequenced the genomes of 13 multidrug-resistant (mdr) a. baumannii strains from taiwan, and compared them with that of a. baumannii from other east asian countries. core-genome phylogenetic tree divided the analyzed str ... | 2016 | 27072398 |
| distribution of drug-resistant bacteria and rational use of clinical antimicrobial agents. | open wound may lead to infection in patients. due to overuse of medication, certain bacteria have become resistant to drugs currently available. the aim of the present study was to provide a guide to ameliorate the appropriate and rational use of clinical antimicrobial agents by analyzing the distribution of drug-resistant pathogenic bacteria in patients. between october 2013 and january 2015, 126 patients were selected at the department of orthopedics. wound secretion samples were collected, an ... | 2016 | 27313667 |
| the intestinal microbiota in metabolic disease. | gut bacteria exert beneficial and harmful effects in metabolic diseases as deduced from the comparison of germfree and conventional mice and from fecal transplantation studies. compositional microbial changes in diseased subjects have been linked to adiposity, type 2 diabetes and dyslipidemia. promotion of an increased expression of intestinal nutrient transporters or a modified lipid and bile acid metabolism by the intestinal microbiota could result in an increased nutrient absorption by the ho ... | 2016 | 27058556 |
| expression of zinc transporter genes in rice as influenced by zinc-solubilizing enterobacter cloacae strain zsb14. | zinc (zn) deficiency in major food crops has been considered as an important factor affecting the crop production and subsequently the human health. rice (oryza sativa) is sensitive to zn deficiency and thereby causes malnutrition to most of the rice-eating asian populations. application of zinc solubilizing bacteria (zsb) could be a sustainable agronomic approach to increase the soil available zn which can mitigate the yield loss and consequently the nutritional quality of rice. understanding t ... | 2016 | 27092162 |
| treatment outcomes of oral sitafloxacin in acute complicated urinary tract infection and pyelonephritis. | data on the success rate of sitafloxacin treatment in acute complicated urinary tract infection and pyelonephritis are limited. | 2016 | 27069830 |
| colitis susceptibility in p47(phox-/-) mice is mediated by the microbiome. | chronic granulomatous disease (cgd) is caused by defects in nicotinamide adenine dinucleotide phosphate oxidase 2 (nox2) complex subunits (gp91(phox) (a.k.a. nox2), p47(phox), p67(phox), p22(phox), p40(phox)) leading to reduced phagocyte-derived reactive oxygen species production. almost half of patients with cgd develop inflammatory bowel disease, and the involvement of the intestinal microbiome in relation to this predisposing immunodeficiency has not been explored. | 2016 | 27044504 |
| simultaneous rapid detection and serotyping of cronobacter sakazakii serotypes o1, o2, and o3 by using specific monoclonal antibodies. | cronobacter sakazakii is a foodborne pathogen associated with rare but often lethal infections in neonates. powdered infant formula (pif) represents the most frequent source of infection. out of the identified serotypes (o1 to o7), o1, o2, and o3 are often isolated from clinical and pif samples. serotype-specific monoclonal antibodies (mabs) suitable for application in enzyme immunoassays (eias) for the rapid detection of c. sakazakii have not yet been developed. in this study, we created specif ... | 2016 | 26850303 |
| age-specific percentile-based reference curve of serum procalcitonin concentrations in japanese preterm infants. | procalcitonin (pct) levels are elevated early after birth in newborn infants; however, the physiological features and reference of serum pct concentrations have not been fully studied in preterm infants. the aims of the current study were to establish an age-specific percentile-based reference curve of serum pct concentrations in preterm infants and determine the features. the pct concentration peaked in infants at 1 day old and decreased thereafter. at 1 day old, serum pct concentrations in pre ... | 2016 | 27033746 |
| clinical practice guideline: management of acute pancreatitis. | there has been an increase in the incidence of acute pancreatitis reported worldwide. despite improvements in access to care, imaging and interventional techniques, acute pancreatitis continues to be associated with significant morbidity and mortality. despite the availability of clinical practice guidelines for the management of acute pancreatitis, recent studies auditing the clinical management of the condition have shown important areas of noncompliance with evidence-based recommendations. th ... | 2016 | 27007094 |
| adenovirus type 7 pneumonia in children who died from measles-associated pneumonia, hanoi, vietnam, 2014. | during a 2014 measles outbreak in vietnam, postmortem pathologic examination of hospitalized children who died showed that adenovirus type 7 pneumonia was a contributory cause of death in children with measles-associated immune suppression. adenovirus type 7 pneumonia should be recognized as a major cause of secondary infection after measles. | 2016 | 26926035 |
| [a rare case of suppurative mesenteric adenitis associated with intussusception in a child: a clinical case]. | some cases of suppurative mesenteric adenitis have already been described in the literature but not associated with intussusception. we describe the case of a 3-year-old boy presenting to the department of surgery at the university hospital of lubumbashi with bowel obstruction. he was visited elsewhere, in the previous 12 days, for diarrhea, vomiting, fever, coma and treated for cerebral malaria and blackwater fever. surgery revealed an ileal intussusception and a suppurative mesenteric adenitis ... | 2016 | 27279973 |
| high prevalence of new delhi metallo-β-lactamase-1 (ndm-1) producers among carbapenem-resistant enterobacteriaceae in kuwait. | the aim of the study was to determine the prevalence of new delhi metallo-β lactamase-1 (ndm-1) producing enterobacteriaceae in kuwait over a one year period. consecutive enterobacteriaceae isolates with reduced susceptibility to carbapenems were collected from four government hospitals in kuwait from january-december 2014. their susceptibility to 18 antibiotics was performed by determining the minimum inhibitory concentration. isolates resistant to carbapenems were tested by pcr for resistant g ... | 2016 | 27031521 |
| distinctly altered gut microbiota in the progression of liver disease. | recent studies underscore important roles of intestinal microbiota and the bacterial lipopolysaccharides (lps) production in the pathogenesis of liver disease. however, how gut microbiota alters in response to the development of steatosis and subsequent progression to nonalcoholic steatohepatitis (nash) and hepatocellular carcinoma (hcc) remains unclear. we aimed to study the gut microbial changes over liver disease progression using a streptozotocin-high fat diet (stz-hfd) induced nash-hcc c57b ... | 2016 | 27036035 |
| quantitative evaluation of extended-spectrum β-lactamase-producing escherichia coli strains in the wastewater of a french teaching hospital and relation to patient strain. | extended-spectrum β-lactamase-producing escherichia coli has become ubiquitous and has been reported in diverse ecosystems. we evaluated the potential impact of post-acute and long-term healthcare activities on the environment by quantifying esbl-producing enterobacteriaceae in wastewaters of a french geriatric hospital. | 2016 | 27030806 |
| antitumor effects of cecropin b-lhrh' on drug-resistant ovarian and endometrial cancer cells. | luteinizing hormone-releasing hormone receptor (lhrhr) represents a promising therapeutic target for treating sex hormone-dependent tumors. we coupled cecropin b, an antimicrobial peptide, to lhrh', a form of lhrh modified at carboxyl-terminal residues 4-10, which binds to lhrhr without interfering with luteinizing hormone (lh) and follicle-stimulating hormone (fsh) secretion. this study aimed to assess the antitumor effects of cecropin b-lhrh' (cb-lhrh') in drug-resistant ovarian and endometria ... | 2016 | 27021903 |
| identification of haplotype tag single-nucleotide polymorphisms within the ppar family genes and their clinical relevance in patients with major trauma. | peroxisome proliferator-activated receptors (ppars) play important roles in the development of inflammatory diseases and sepsis. recently, genetic variants of ppars genes have been widely studied in some inflammatory diseases. however, the association between ppar family of genes polymorphisms and sepsis risk in trauma patients was little known. | 2016 | 27023591 |
| community-onset extended-spectrum β-lactamase-producing enterobacteriaceae invasive infections in children in a university hospital in france. | limited data is available on pediatric community-onset infections with extended-spectrum β-lactamase-producing enterobacteriaceae (esbl-pe), but such infections may affect both the efficacy of empiric antibiotic therapy and the rational use of antibiotics.we retrospectively analyzed data from 2007 to 2012 for all children ≤16 years old with a positive esbl-pe strain from usually sterile sites within 48 hours of admission in a tertiary hospital in france. we analyzed healthcare- and community-ass ... | 2016 | 27015202 |
| multiplex antibody detection for noninvasive genus-level diagnosis of prosthetic joint infection. | we developed and evaluated a multiplex antibody detection-based immunoassay for the diagnosis of prosthetic joint infections (pjis). sixteen protein antigens from three staphylococcusspecies (staphylococcus aureus,staphylococcus epidermidis, and staphylococcus lugdunensis) (8 antigens),streptococcus agalactiae(4 antigens), and propionibacterium acnes(4 antigens) were selected by comparative immune proteomics using serum samples from pji cases versus controls. a bead-based multiplex immunoassay t ... | 2016 | 26865683 |
| association of novel nonsynonymous single nucleotide polymorphisms in ampd with cephalosporin resistance and phylogenetic variations in ampc, ampr, ompf, and ompc in enterobacter cloacae isolates that are highly resistant to carbapenems. | inenterobacter cloacae, the genetic lesions associated with derepression of the ampc β-lactamase include diverse single nucleotide polymorphisms (snps) and/or indels in theampdandamprgenes and snps inampc, while diverse snps in the promoter region or snps/indels within the coding sequence of outer membrane proteins have been described to alter porin production leading to carbapenem resistance. we sought to define the underlying mechanisms conferring cephalosporin and carbapenem resistance in a c ... | 2016 | 26856839 |
| survey of carbapenemase-producing enterobacteriaceae in companion dogs in madrid, spain. | we found a low prevalence (0.6%) of carbapenemase-producingenterobacteriaceae(cpe) in fecal microbiota of companion dogs. a single vim-1-producingklebsiella pneumoniaeisolate belonging to sequence type (st) 2090 was detected.blavim-1was carried on a class 1 integron and an untypeable ∼48-kb plasmid. emergence and spread of cpe in this group of animals may be a threat to public health in human and veterinary medicine. this finding supports the need for active surveillance studies in companion ani ... | 2016 | 26824947 |
| prediction of fluoroquinolone resistance in gram-negative bacteria causing bloodstream infections. | increasing rates of fluoroquinolone resistance (fq-r) have limited empirical treatment options for gram-negative infections, particularly in patients with severe beta-lactam allergy. this case-control study aims to develop a clinical risk score to predict the probability of fq-r in gram-negative bloodstream isolates. adult patients with gram-negative bloodstream infections (bsi) hospitalized at palmetto health system in columbia, south carolina, from 2010 to 2013 were identified. multivariate lo ... | 2016 | 26833166 |
| landscape of resistance-nodulation-cell division (rnd)-type efflux pumps in enterobacter cloacae complex. | in gram-negative bacteria, the active efflux is an important mechanism of antimicrobial resistance, but little is known about theenterobacter cloacaecomplex (ecc). it is mediated primarily by pumps belonging to the rnd (resistance-nodulation-cell division) family, and only acrb, part of the acrab-tolc tripartite system, was characterized in ecc. however, detailed genome sequence analysis of the straine. cloacaesubsp.cloacaeatcc 13047 revealed to us that 10 other genes putatively coded for rnd-ty ... | 2016 | 26856831 |
| preventing implant-associated infections by silver coating. | implant-associated infections (iais) are a dreaded complication mainly caused by biofilm-forming staphylococci. implant surfaces preventing microbial colonization would be desirable. we examined the preventive effect of a silver-coated titanium-aluminum-niobium (tialnb) alloy. the surface elicited a strong, inoculum-dependent activity againststaphylococcus epidermidisandstaphylococcus aureusin an agar inhibition assay. gamma sterilization and alcohol disinfection did not alter the effect. in a t ... | 2016 | 26883700 |
| hemotin, a regulator of phagocytosis encoded by a small orf and conserved across metazoans. | translation of hundreds of small orfs (smorfs) of less than 100 amino acids has recently been revealed in vertebrates and drosophila. some of these peptides have essential and conserved cellular functions. in drosophila, we have predicted a particular smorf class encoding ~80 aa hydrophobic peptides, which may function in membranes and cell organelles. here, we characterise hemotin, a gene encoding an 88aa transmembrane smorf peptide localised to early endosomes in drosophila macrophages. hemoti ... | 2016 | 27015288 |
| induced bacterial cross-resistance toward host antimicrobial peptides: a worrying phenomenon. | bacterial resistance to conventional antibiotics has reached alarming levels, threatening to return to the pre-antibiotic era. therefore, the search for new antimicrobial compounds that overcome the resistance phenomenon has become a priority. antimicrobial peptides (amps) appear as one of the most promising antibiotic medicines. however, in recent years several amp-resistance mechanisms have been described. moreover, the amp-resistance phenomenon has become more complex due to its association w ... | 2016 | 27047486 |
| cd64 and group ii secretory phospholipase a2 (spla2-iia) as biomarkers for distinguishing adult sepsis and bacterial infections in the emergency department. | early diagnosis of sepsis and bacterial infection is imperative as treatment relies on early antibiotic administration. there is a need to develop new biomarkers to detect patients with sepsis and bacterial infection as early as possible, thereby enabling prompt antibiotic treatment and improving the survival rate. | 2016 | 27003588 |
| interleukin-6 kinetics can be useful for early treatment monitoring of severe bacterial sepsis and septic shock. | early appropriate anti-microbial therapy is necessary to improve outcomes of septic patients. we describe 20 case histories of patients with severe bacterial sepsis regarding kinetics of several biomarkers. we found that interleukin-6 is able to predict survival and might be able to evaluate appropriateness of anti-microbial therapy. | 2016 | 27103972 |
| clinical significance of community- and healthcare-acquired carbapenem-resistant enterobacteriaceae isolates. | this study was conducted to investigate the clinical significance, manifestations, microbiological characteristics and outcomes of carbapenem-resistant enterobacteriaceae (cre) isolates, and compare the clinical features of community- and healthcare-acquired cre isolates. a total of 78 patients were identified to have cre. klebsiella pneumoniae was the most common pathogens (n = 42, 53.8%), followed by enterobacter cloacae (n = 24, 30.8%), and escherichia coli (n = 11, 14.1%). most of the patien ... | 2016 | 26999356 |
| negative short-term impact of intraoperative biliary lavage in patients with hepatolithiasis. | to evaluate short-term outcomes following intraoperative biliary lavage for hepatolithiasis. | 2016 | 27004001 |
| tigecycline treatment for carbapenem-resistant enterobacteriaceae infections: a systematic review and meta-analysis. | carbapenem-resistant enterobacteriaceae (cre) infections are prevalent worldwide; they have few effective treatments and this jeopardizes public health. clinicians often use tigecycline to combat cre, but its clinical efficacy remains controversial. therefore, to compare the efficacy and safety of tigecycline in treating cre infections compared with that of other antimicrobial agents, and to evaluate whether combination therapy and high-dose regimens are beneficial, we performed a systematic rev ... | 2016 | 26986165 |
| metagenomic sequencing with strain-level resolution implicates uropathogenic e. coli in necrotizing enterocolitis and mortality in preterm infants. | necrotizing enterocolitis (nec) afflicts approximately 10% of extremely preterm infants with high fatality. inappropriate bacterial colonization with enterobacteriaceae is implicated, but no specific pathogen has been identified. we identify uropathogenic e. coli (upec) colonization as a significant risk factor for the development of nec and subsequent mortality. we describe a large-scale deep shotgun metagenomic sequence analysis of the early intestinal microbiome of 144 preterm and 22 term inf ... | 2016 | 26997279 |
| health care associated infections, antibiotic resistance and clinical outcome: a surveillance study from sanandaj, iran. | to study the antibiotic susceptibility patterns of gram-negative healthcare associated bacterial infections at two tertiary hospitals in the sanandaj city, kurdistan province, iran. | 2016 | 26989670 |
| progression of benign prostatic hyperplasia is associated with pro-inflammatory mediators and chronic activation of prostate-infiltrating lymphocytes. | benign prostatic hyperplasia (bph) is a common chronic non-malignant condition whose prevalence substantially increases with age. immune cell infiltration and pro-inflammatory mediators have been implicated in the pathogenesis. here, we characterized 21 extracellular markers on prostate-infiltrating lymphocytes (pils) and analyzed expression of 26 soluble proteins in prostate tissue obtained from bph patients (n = 31). these data were correlated with clinical parameters and compared with periphe ... | 2016 | 26993768 |
| microbiological assessment of bile and corresponding antibiotic treatment: a strobe-compliant observational study of 1401 endoscopic retrograde cholangiographies. | the aim of this study was to determine the antibiotic susceptibility profiles of bacteria in bile samples and to analyze the clinical relevance of the findings as only limited information about risk factors for elevated frequence of bacterial and fungal strains in routinely collected bile samples has been described so far.a prospective cohort study at a tertiary care center was conducted. seven hundred forty-four patients underwent 1401 endoscopic retrograde cholangiographies (ercs) as indicated ... | 2016 | 26962768 |
| genotypic and phenotypic detection of ampc β-lactamases in enterobacter spp. isolated from a teaching hospital in malaysia. | the objective of this study was to determine the occurrence of chromosomal and plasmid-mediated β-lactamases (ampc) genes in a collection of malaysian isolates of enterobacter species. several phenotypic tests for detection of ampc production of enterobacter spp. were evaluated and the agreements between tests were determined. | 2016 | 26963619 |