Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| utility of 5 major putative periodontal pathogens and selected clinical parameters to predict periodontal breakdown in patients on maintenance care. | the predictive utility of 5 major putative periodontopathic microbial species, "superinfecting" organisms, and several clinical periodontal parameters were assessed relative to periodontitis recurrence over a 12-month period in 78 treated adult patients participating in a 3-month maintenance care program. at baseline, pooled subgingival microbial samples were collected from each patient, and whole-mouth evaluations of probing depth, relative periodontal attachment level, furcation involvement, a ... | 1996 | 8739166 |
| treatment of periodontal disease based on microbiological diagnosis. relation between microbiological and clinical parameters during 5 years. | the purpose of this study was to assess the clinical effect of treatment aimed to suppress actinobacillus actinomycetemcomitans and porphyromonas gingivalis below detection level and prevotella intermedia below 5% of the cultivable periodontal pocket flora. sixteen patients and a total of 111 periodontal pockets with probing depth > or = 6 mm were included in the study. twelve patients and a total of 77 sites completed the 5-year study. the results demonstrated clinical improvement of probing de ... | 1996 | 8794965 |
| source of suspected periodontal pathogens re-emerging after periodontal treatment. | to clarify the source of re-emerging periodontal pathogens after treatment, we compared the ribotypes of actinobacillus actinomycetemcomitans, porphyromonas gingivalis, prevotella intermedia/prevotella nigrescens group and campylobacter rectus before and after treatment in 7 periodontitis patients and in 6 of the spouses. the patients harbored a. actinomycetemcomitans, p. gingivalis, p. intermedia/p. nigrescens group or c. rectus in their subgingival or salivary samples before treatment. the res ... | 1996 | 8811482 |
| evaluation of the long-term efficacy and safety of locally-applied minocycline in adult periodontitis patients. | the objectives of the present study were to establish in a long-term investigation the safety as well as the clinical and microbiological efficacy of scaling and rootplaning combined with local application of 2% minocycline hydrochloride-gel versus placebo-gel in patients with moderate to severe chronic adult periodontitis. this was an 18 months, randomized, double-blind, parallel, comparative study, in which 20 healthy patients with moderate to severe chronic periodontitis participated. at base ... | 1996 | 8877655 |
| selling biotechnology in the dental medicine marketplace: the omnigene diagnostics dna probe tests for periodontal pathogens. | omnigene diagnostics, inc. has applied the principles of genetic engineering to develop species-specific dna probe tests for eight periodontal pathogens (porphyromonas gingivalis, prevotella intermedia, actinobacillus actinomycetem-comitans, fusobacterium nucleatum, eikenella corrodens, campylobacter rectus, bacteroides forsythus, and treponema denticola). the test requires minimal effort on the part of the clinician: subgingival plaque samples are collected from the patient and sent through the ... | 1996 | 8931245 |
| associations between porphyromonas gingivalis and oral treponemes in subgingival plaque. | colonization and/or proliferation of treponema denticola may depend on the presence of porphyromonas gingivalis. the aims of this study were to confirm this synergistic relationship, to determine whether other oral bacteria were similarly associated with p. gingivalis and to relate coinfection to the periodontal status of plaque donors. subgingival plaque was collected from every tooth except third molars in 106 subjects who were grouped by their worst periodontal condition. in addition to p. gi ... | 1996 | 8941768 |
| the adherence of periodontopathogens to periodontal probes. a possible factor in intra-oral transmission? | periodontal probes have previously been shown to harbor several bacterial types or species after probing periodontally diseased pockets. this study aims to identify and quantify periodontopathogens that may adhere to a periodontal probe by culturing techniques. it also examines the probe's roughness on its capability to collect bacteria, comparing merrit-b probes (with deep indentations) with tps probes (with smooth surfaces). from the differential phase contrast microscopy findings it was seen ... | 1996 | 8959565 |
| degradation of lactoferrin by periodontitis-associated bacteria. | the degradation of human lactoferrin by putative periodontopathogenic bacteria was examined. fragments of lactoferrin were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and measured by densitometry. the degradation of lactoferrin was more extensive by porphyromonas gingivalis and capnocytophaga sputigena, slow by capnocytophaga ochracea, actinobacillus actinomycetemcomitans and prevotella intermedia, and very slow or absent by prevotella nigrescens, campylobacter rectus ... | 1996 | 8961558 |
| polymerase chain reaction detection of 8 putative periodontal pathogens in subgingival plaque of gingivitis and advanced periodontitis lesions. | a 16s rrna-based polymerase chain reaction (pcr) detection method was used to determine the prevalence of actinobacillus actinomycetemcomitans, bacteroides forsythus, campylobacter rectus, eikenella corrodens, porphyromonas gingivalis, prevotella intermedia. prevotella nigrescens and treponema denticola in subgingival specimens of 50 advanced periodontitis, 50 adult gingivitis and 50 pediatric gingivitis subjects. the optimal pcr conditions were determined for each study species. agarose gel ele ... | 1996 | 9002880 |
| plaque ph and microflora of dental plaque on sound and carious root surfaces. | given the hypothesis that root caries is the result of acid formation by acidogenic micro-organisms, the present study was performed to relate sucrose-induced ph response of dental plaque on root surfaces to the microbial composition of the overlying plaque. seventeen caries-active elderly chinese with poor oral hygiene and with both sound and carious root surfaces were examined. plaque ph was measured before and up to one hour after a controlled sucrose mouthrinse. plaque samples for microbiolo ... | 1996 | 9003238 |
| in vitro senescence enhances il-6 production in human gingival fibroblasts induced by lipopolysaccharide from campylobacter rectus. | the production of interleukin-6 (il-6) in human gingival fibroblasts (gin cells) is increased by lipopolysaccharide (lps) from campylobacter rectus (c. rectus), which is associated with adult periodontitis; however, the age-related changes in the susceptibility of gin cells to c. rectus lps remain unclear. we examined the influence of in vitro senescence on c. rectus lps-stimulated il-6 production in gin cells. lps was prepared from c. rectus atcc 33238 using hot phenol-water. the gin cells were ... | 1996 | 8735906 |
| periodontal status and detection frequency of bacteria at sites of periodontal health and gingivitis. | it is generally recognized that bacteria in dental plaque at sites of periodontal diseases are not commonly found at sites of periodontal health. one hypothesis to explain the etiology of periodontitis is that pathogenic bacteria from diseased sites infect healthy sites. it has been suggested that gingival inflammation may predispose sites to colonization by bacteria associated with periodontal diseases. the purpose of this cross-sectional study was to determine whether the detection frequency o ... | 1996 | 8667130 |
| comparison of randomly cloned and whole genomic dna probes for the detection of porphyromonas gingivalis and bacteroides forsythus. | whole genomic and randomly-cloned dna probes for two fastidious periodontal pathogens, porphyromonas gingivalis and bacteroides forsythus were labeled with digoxigenin and detected by a colorimetric method. the specificity and sensitivity of the whole genomic and cloned probes were compared. the cloned probes were highly specific compared to the whole genomic probes. a significant degree of cross-reactivity with bacteroides species, capnocytophaga sp. and prevotella sp. was observed with the who ... | 1996 | 8636873 |
| in-vitro antibacterial effects of extracts of nigerian tooth-cleaning sticks on periodontopathic bacteria. | aqueous extracts from 8 plants used for tooth-cleaning in nigeria were tested for their ability to inhibit the growth of five periodontopathic bacteria, porphyromonas gingivalis, prevotella intermedia, fusobacterium nucleatum, eikenella corrodens and campylobacter rectus. extracts of all the plants except that of massularia acuminata exhibited varying growth inhibitory potentials on the microorganisms. extract of terminalia glaucescens showed the widest spectrum of activity, inhibiting the growt ... | 1995 | 9590895 |
| salivary levels of suspected periodontal pathogens in relation to periodontal status and treatment. | the primary ecological niche for suspected periodontal pathogens seems to be the subgingival area, even though periodontal pathogens are also frequently recovered from saliva. the interrelationship of different periodontal conditions and the salivary levels of suspected periodontal pathogens is not known. in the present study, salivary levels of actinobacillus actinomycetemcomitans, porphyromonas gingivalis, prevotella intermedia, campylobacter rectus, and peptostreptococcus micros were determin ... | 1995 | 8530742 |
| oral microbiota in subjects with a weak or strong response in experimental gingivitis. | the purpose of the present study was to examine the composition of the oral microbiota in subjects who had previously demonstrated to develop either a weak or strong response to experimental gingivitis. for this study, subjects were selected from a pool of 25 individuals who had participated twice in an experimental gingivitis trial. out of these 25 panellists, 6 subjects were selected who had developed 2x a weak gingival inflammatory response and 7 subjects who had developed 2x a strong gingiva ... | 1995 | 8583022 |
| cell envelope and cell wall immunization of macaca fascicularis: effect on the progression of ligature-induced periodontitis. | the nonhuman primate, macaca fascicularis, was used to study the role of immunization with selected members of the periodontopathic microbiota in the longitudinal progression of ligature-induced periodontitis. animals were immunized with cell envelope antigens prepared from porphyromonas gingivalis and prevotella intermedia, and a mixture prepared from fusobacterium nucleatum, campylobacter rectus, and actinomyces viscosus. serum immunoglobulin g (igg), igm and iga isotype antibodies increased s ... | 1995 | 8602339 |
| periodontal findings in spouses. a clinical, radiographic and microbiological study. | clinical, radiographic and microbiological examination of periodontal conditions was carried out in 2 groups of married couples to assess similarities between husband and wife. the diseased probands (n = 10) exhibited advanced periodontitis and the healthy ones (n = 10) were periodontally normal. the clinical examination comprised the assessment of plaque, probing pocket depths, gingival bleeding on probing, suppuration, supragingival and subgingival calculus. the extent and type of alveolar bon ... | 1995 | 7775676 |
| effect of campylobacter rectus lps on plasminogen activator-plasmin system in human gingival fibroblast cells. | the plasminogen activator (pa)-plasmin system is implicated in the degradation of the extracellular matrix in inflammation through activation of metalloproteases and prekallikrein. we examined the activation of the pa-plasmin system in human gingival fibroblast cells (gin-1 cells) following treatment with lipopolysaccharide (lps) from campylobacter rectus, which is frequently detected at sites of periodontal disease. the c. rectus lps stimulated the plasmin activity in the conditioned medium of ... | 1995 | 7776154 |
| periodontopathogens in elderly chinese with different periodontal disease experience. | if an etiological relationship exists between destructive periodontal disease and putative periodontopathogens, they would be expected to have a very low prevalence in periodontally healthy elderly persons. to test this hypothesis, 2 subgroups of elderly, rural chinese (a periodontally "best" and a "worst" group, each comprising 15 persons) were identified in 1990 from a cohort aged 55-69 years, examined in 1984. assessment of changes in periodontal status over the 6-year period were possible by ... | 1995 | 7790524 |
| evaluation of periodontal treatments using controlled-release tetracycline fibers: microbiological response. | in a 12-month multi-center study of 116 adult periodontitis subjects, six putative periodontal pathogens were monitored by dna probe methods in a subset of 31 subjects. monitored species included porphyromonas gingivalis (pg), prevotella intermedia (pi), fusobacterium nucleatum (fn), eikenella corrodens (ec), campylobacter rectus (cr), and actinobacillus actinomycetemcomitans (aa) with an average detection limit of 1.8 x 10(4) bacterial colony forming units/sample. the microbiological response t ... | 1995 | 7473013 |
| energetics of molecular hydrogen oxidation in the oral pathogen campylobacter rectus. | 1995 | 7548544 | |
| detection of putative periodontal pathogens in subgingival specimens by 16s ribosomal dna amplification with the polymerase chain reaction. | the utility of the 16s ribosomal rna-based polymerase chain reaction (pcr) for detection of actinobacillus actinomycetemcomitans, bacteroides forsythus, campylobacter rectus, eikenella corrodens, porphyromonas gingivalis, prevotella intermedia, and treponema denticola was examined and compared with that of anaerobic culture. primer pairs consisting of 20-27 nucleotides amplified 404- to 688-bp regions of 16s ribosomal rna genes of these organisms. this method had a lower detection limit of 50 ta ... | 1995 | 7548580 |
| some suspected periodontopathogens and serum antibody response in adult long-duration insulin-dependent diabetics. | the subgingival microflora and serum antibody response were examined in long-duration insulin-dependent diabetics and age- and sex-matched non-diabetics. the material consisted of 9 diabetics aged 40-49 years and 19 aged 50-59 years, 13 non-diabetics aged 40-49 years and 21 aged 50-59 years. the bacterial species studied (actinobacillus actinomycetemcomitans, campylobacter rectus, capnocytophaga spp, eikenella corrodens, fusobacterium nucleatum, porphyromonas gingivalis, prevotella intermedia) w ... | 1995 | 7560223 |
| occurrence of certain bacterial species and morphotypes in juvenile periodontitis in chile. | the occurrence of actinobacillus actinomycetemcomitans, porphyromonas gingivalis, prevotella intermedia, eikenella corrodens, fusobacterium nucleatum, campylobacter rectus, capnocytophaga species, and certain bacterial morphotypes was determined in 18 affected and 18 unaffected sites in 10 localized juvenile periodontitis (ljp) patients, and in 10 affected and 10 unaffected sites in 5 generalized juvenile periodontitis (gjp) patients. the subgingival proportion of the 7 bacterial species was det ... | 1995 | 7562347 |
| periodontal microbiota of mobile and non-mobile teeth. | the mechanism of accelerated periodontal destruction around teeth with occlusal trauma and increased mobility remains unclear. one possibility is that tooth mobility creates a subgingival environment conducive to overgrowth by periodontal pathogens. this study compared the subgingival microflora in mobile and non-mobile teeth of 35 adults on supportive maintenance therapy and 15 with untreated adult periodontitis. in each subject, subgingival paper-point samples were obtained from a mobile tooth ... | 1995 | 7623258 |
| clinical and laboratory studies on a patient with early onset periodontitis and her family members. a case report. | extensive clinical, microbiological, hematological, and immunological studies were performed on a patient with early onset periodontitis (eop) and two other members of the family. the proband, a 27-year-old female, had early onset periodontitis and a high level of serum rheumatoid factors (rf) with no diagnosable medical disease. her mother had lost all her teeth at the age of 50 because of advanced periodontitis, while her elder sister was unaffected by periodontitis. neither the proband's peri ... | 1995 | 7623261 |
| effect of treatment on some periodontopathogens and their antibody levels in periodontal abscesses. | twenty patients suffering from abscess of periodontal origin were treated and followed clinically and microbiologically for 6 months. microbiological examination was performed by culture technique. one deep and one shallow periodontal pocket in the same patient were examined concomitantly. serum was collected and analyzed for specific antibody level using elisa methodology. treatment included supragingival scaling, drainage, and irrigation of the periodontal pocket with 0.85% sodium chloride and ... | 1994 | 7853125 |
| the gingival immune response to periodontal pathogens in juvenile periodontitis. | a gingival explant culture system was utilized to evaluate the reactivity of local immunoglobulins produced by juvenile periodontitis tissue. gingival explant culture supernatant fluids were screened, via a standardized dot-immunobinding assay, for antibodies reactive to: actinobacillus actinomycetemcomitans, porphyromonas gingivalis, prevotella intermedia, campylobacter rectus, eikenella corrodens, peptostreptococcus micros, peptostreptococcus anaerobius, capnocytophaga ochracea, eubacterium no ... | 1994 | 7870467 |
| macaca fascicularis as a model in which to assess the safety and efficacy of a vaccine for periodontitis. | we have assessed macaca fascicularis as a potential model in which to test the efficacy and safety of a vaccine for periodontitis. twenty-eight animals were surveyed and 20 studied in more detail. clinical periodontal status was assessed, the subgingival microflora analyzed especially for the presence and proportions of porphyromonas gingivalis and titers and avidities of serum antibodies reactive with p. gingivalis measured. probing depths ranged from 0.90 mm to 3.80 mm, gingival index scores f ... | 1994 | 8008428 |
| interdental supragingival plaque--a natural habitat of actinobacillus actinomycetemcomitans, bacteroides forsythus, campylobacter rectus, and prevotella nigrescens. | the aim of this study was to test the hypothesis that suspected periodontal pathogens form a minor component of the supragingival plaque of individuals without periodontal diseases. twenty-one dental hygienist trainees with a mean age of 23.5 years were twice sampled for interdental plaque between 1st and 2nd molars in all quadrants. the samples were assessed for actinobacillus actinomycetemcomitans, bacteroides forsythus, campylobacter rectus, porphyromonas gingivalis, and the prevotella interm ... | 1994 | 8083438 |
| detection of porphyromonas gingivalis in gingival exudate by a dipeptide-enhanced trypsin-like activity. | porphyromonas gingivalis in subgingival plaque is an important risk factor for future periodontal attachment loss in susceptible adults. the elimination of p. gingivalis is usually concomitant with a healing process. therefore, it should be valuable to have an easy chairside method to follow the effect of periodontal treatment on p. gingivalis detection as well as on its eventual reappearance during the maintenance period. we have previously reported the stimulation of amidolytic activity of p. ... | 1994 | 8133415 |
| relationship between salivary blood group antigens, microbial flora and periodontal condition in young adults. | to investigate a possible rôle for salivary blood-group antigens in the relative frequencies of selected periodontal pathogens and commensal oral micro-organisms, we studied the clinical and microbiological condition in young adults with or without blood group reactive substances in saliva (secretors or non-secretors respectively). clinical measurements were recorded at the ramfjord teeth in 81 1st-year dental students. in addition, presence of interproximal loss of attachment (la) was assessed ... | 1994 | 8157769 |
| flow-cytometric identification and detection of porphyromonas gingivalis by a lps specific monoclonal antibody. | the purpose of this study was to identify porphyromonas gingivalis (p. gingivalis) by flow cytometry (fcm) using a monoclonal antibody (mab) omr-bg1e directed to p. gingivalis-specific lipopolysaccharide (lps). the p. gingivalis strains atcc 33277, 381, eso75, w50, and a7a1 were selected for the study. fusobacterium nucleatum (f. nucleatum), prevotella intermedia (p. intermedia), campylobacter rectus (c. rectus), streptococcus sanguis (s. sanguis) and actinobacillus actinomycetemcomitans (a. act ... | 1994 | 8195974 |
| impaired bactericidal activity of pmn from two brothers with necrotizing ulcerative gingivo-periodontitis. | the purpose of this study was to investigate the pathogenesis of necrotizing ulcerative gingivo-periodontitis (anup) diagnosed in two brothers, age 9 (anup1) and 14 (anup2) from rural egypt. complete blood count, differential and blood chemistry were within normal limits for both brothers and they were not malnourished. the phagocytosis and killing function of their polymorphonuclear leukocytes (pmn) towards four bacterial species were assessed using a fluorochrome microassay. the selection of b ... | 1994 | 8195981 |
| bacteriological diagnosis of periodontal disease. | dental plaque bacteria cause virtually all the forms of inflammatory periodontal disease. periodontitis is caused by the specific periodontopathic bacteria, which induce destruction of connective tissue attachment and adjacent alveolar bone. examinations to identify the infections by porphyromonas gingivalis, actinobacillus actinomycetemcomitans, prevotella intermedia, campylobacter rectus, bacteroides forsythus, fusobacterium nucleatum, eikenella corrodens and treponema denticola have recently ... | 1994 | 8620588 |
| effect of initial therapy on dynamics of immunogloblin g levels to some periodontopathic bacteria in serum and gingival crevicular fluid. | using enzyme-linked immunosorbent assay, the igg antibody levels to specific gram-negative periodontopathic bacteria in serum of 21 adult periodontitis patients and the gingival crevicular fluid (gcf) from 42 sites with destructive lesions and 21 healthy sites were evaluated before and after initial preparation. porphyromonas gingivalis, bacteroides forsythus, prevotella intermedia, prevotella nigrescens, campylobacter rectus, treponema denticola and actinobacillus actinomycetemcomitans in subgi ... | 1994 | 8689734 |
| identification and analyses of periodontal pathogens in taiwan by microbiological tests. | the purpose of this study was to use microbiological tests for diagnosis of periodontal diseases in taiwan. anaerobic culture, direct microscopy, indirect immunofluorescence (if), and biochemical tests were used to examine 336 samples for the specific microorganisms in subgingival plaque. the results indicated that gram-negative species and motile bacteria were less frequently detected, and in lower proportion, in samples from healthy sites. the bacteria found frequently in healthy group were th ... | 1994 | 9747334 |
| sacroiliitis and septicemia caused by campylobacter rectus and actinomyces odontolyticus. | campylobacter rectus, formerly known as wolinella recta, is an anaerobic gram-negative bacillus, generally recognized as an agent responsible for severe periodontitis; only two cases of extra-oral infections have been reported. the first case of septicemia with c rectus and actinomyces odontolyticus is described in a 37-year-old farmer who suffered from severe sacroiliitis. also presented are a review of c rectus in human pathology, and a brief review of pyogenic sacroiliitis, a rather rare dise ... | 1994 | 22346489 |
| microbial associations in periodontitis sites before and after treatment. | duplicate samples from 110 periodontal sites of 6 mm or more pocket depth in 16 patients were analyzed for the presence of actinobacillus actinomycetemcomitans, porphyromonas gingivalis, prevotella intermedia, capnocytophaga spp., campylobacter rectus, eikenella corrodens and fusobacterium nucleatum. the sites were sampled before and after nonsurgical periodontal treatment. no statistically significant associations were found before treatment between any of the analyzed species. after treatment, ... | 1993 | 8247608 |
| role of suspected periodontopathogens in microbiological monitoring of periodontitis. | periodontal disease is the clinical result of a complex interaction between the host and plaque bacteria. although a specificity to some degree is found for actinobacillus actinomycetemcomitans in localized juvenile periodontitis (ljp), it has been difficult to obtain evidence for a specific etiological role of the bacteria associated with periodontal disease in adults. what we see is the net result of host-parasite interactions which in an unpredictable moment accumulate and exceed the threshol ... | 1993 | 8260004 |
| dna probe detection of periodontopathogens in advanced periodontitis. | species-specific dna probes were used to determine the presence of actinobacillus actinomycetemcomitans (a.a.), porphyromonas (bacteroides) gingivalis, prevotella intermedia, treponema denticola, eikenella corrodens, fusobacterium nucleatum, and wolinella recta in subgingival plaque from deep pockets/sites of patients with advanced periodontitis. the subjects were 20 patients with severe adult periodontitis, 13 men and 7 women (mean age 45.6 +/- 6.7 yr). for each subject, 9-10 subgingival sites ... | 1993 | 8290878 |
| isolation and partial characterization of the campylobacter rectus cytotoxin. | previously, we reported the antigranulocytic activity of campylobacter rectus media supernatants containing lipopolysaccharide (lps) and a 104 kda protein. here, we monitored the release of protein and lps through the growth cycle of c. rectus atcc 33238 and identified the 104 kda protein as the cytotoxin. lps in media supernatants was quantitated by a kdo assay; the 104 kda protein was detected on immunoblots with specific antibody (a104) and quantitated by amino acid analysis of membrane immob ... | 1993 | 8321122 |
| recent taxonomic changes for anaerobic gram-positive and selected gram-negative organisms. | the strains formerly classified as streptococcus anginosus or "streptococcus milleri" have now been recognized as three distinct species, streptococcus anginosus, streptococcus constellatus, and streptococcus intermedius. streptococcus morbillorum has been transferred into the genus gemella. four new species within the genus peptostreptococcus were recently named. a recent addition to the genus clostridium is clostridium argentinense, which includes clostridium botulinum type g. two new species ... | 1993 | 8324113 |
| oral and dental infections with anaerobic bacteria: clinical features, predominant pathogens, and treatment. | microbial populations colonizing the teeth are a major source of pathogens responsible for oral and dental infections, including periodontal diseases, gingivitis, pericoronitis, endodontitis, peri-implantitis, and postextraction infections. each entity has distinct clinical and microbial features. bacterial species associated with oral infections include actinobacillus actinomycetemcomitans, porphyromonas gingivalis, prevotella intermedia, bacteroides forsythus, campylobacter rectus, eubacterium ... | 1993 | 8324136 |
| suppression of proliferation of a human b-cell leukaemic cell line derived from acute lymphoblastic leukaemia by soluble factor(s) from campylobacter rectus. | soluble sonic extracts of several strains were examined for their ability to alter proliferation of a cell line derived from acute lymphoblastic leukaemia (ball-1). extracts of all strains tested caused dose-dependent suppression of proliferation when assessed by dna (tritiated thymidine incorporation), rna (tritiated uridine incorporation) and protein (tritiated leucine incorporation) synthesis. there was no effect on the viability of ball-1 as measured by either trypan-blue exclusion or extrac ... | 1993 | 8343067 |
| helicobacter acinonyx sp. nov., isolated from cheetahs with gastritis. | four strains of a novel helicobacter species were isolated from the stomachs of cheetahs (acinonyx jubilatus) with gastritis. these isolates were phenotypically similar to helicobacter pylori. the isolates were gram-negative, spiral bacteria which grew under microaerophilic conditions at 37 degrees c, but not at 25 or 42 degrees c, and produced urease, catalase, oxidase, alkaline phosphatase, and gamma-glutamyl transpeptidase. the isolates did not ferment glucose, mannitol, inositol, sorbitol, r ... | 1993 | 8379970 |
| microbiological and clinical monitoring of non-localized juvenile periodontitis in young adults: a report of 11 cases. | it has been shown that patients with localized juvenile periodontitis (ljp) often harbor actinobacillus actinomycetemcomitans in the subgingival area. however, little is known of the oral microflora in non-ljp juvenile periodontitis patients with less extensive disease. the purpose of this study was to describe the microflora and clinical parameters of young adults with minor to moderate periodontitis during treatment for a period of 1 year. eleven patients 15 to 16 years of age were studied. al ... | 1993 | 8381179 |
| effect of therapy on periodontal infections. | periodontal disease progression requires the simultaneous presence of high numbers of pathogens, low numbers of compatible or beneficial species, a conductive local environment, and a susceptible host. effective therapy acts by altering one or more of these factors. data from an ongoing study were used to examine the biological basis of treatment success or failure. seventeen subjects showing disease progression were treated by widman flap surgery at deep sites, scaling at shallow sites, and 1 o ... | 1993 | 8410615 |
| microbial composition and pattern of antibiotic resistance in subgingival microbial samples from patients with refractory periodontitis. | a series of 993 subgingival microbial samples sent to a diagnostic microbiology laboratory included 196 samples that could be identified as compatible with a clinical diagnosis of refractory or recurrent periodontitis. in descending order of prevalence the associated microbiota included bacteroides forsythus (84%), spirochetes (83%), motile rods (76%), fusobacterium species (68%), porphyromonas gingivalis (63%), campylobacter rectus (47%), capnocytophaga species (38%), prevotella intermedia (23% ... | 1993 | 8463936 |
| an analysis of the subgingival microflora in randomly selected subjects. | the aim of this study was to describe the presence of some microbial species in the subgingival microflora of a randomly selected subject sample. a further objective was to analyze the association between some microbiological characteristics and the periodontal conditions of the subjects. a total of 171 individuals aged 30-65 years were included. a pooled subgingival plaque sample was obtained from the mesial aspect of the 6 ramfjord teeth by the use of the paper-point method. the samples were p ... | 1993 | 8510980 |
| aspartame as a source of essential phenylalanine for the growth of oral anaerobes. | phenylalanine and aspartic acid requirements were determined for 13 species of oral bacteria using the chemically defined medium omiz-w1. none of actinobacillus actinomycetemcomitans, bacteroides forsythus, eikenella corrodens, selenomonas sputigena, treponema pectinovorum, t. socranskii, or wolinella recta required either of these amino acid constituents of aspartame (l-aspartyl-l-phenylalanine methylester). phenylalanine was essential for the growth of capnocytophaga gingivalis, eubacterium ti ... | 1993 | 8514113 |
| antigenic properties of campylobacter rectus (wolinella recta) major s-layer proteins. | the antigenic properties of the surface layer (s-layer) proteins of various campylobacter rectus strains including 24 clinical isolates and the type strain atcc 33238 were examined. s-layer proteins were extracted from whole cells by acid treatment according to the method of mccoy et al. (infect. immun. 11, 517-525, 1975). the acid extracts from 23 of the isolates and atcc 33238 contained two major proteins with molecular masses of 130 kda and 150 kda, both of which were identified as subunits o ... | 1993 | 8514115 |
| campylobacter showae sp. nov., isolated from the human oral cavity. | nine campylobacter-like strains were isolated from human gingival crevices and characterized. these strains were gram-negative, straight rods that were motile by means of multiple unipolar flagella. they were asaccharolytic and preferred an anaerobic atmosphere rather than a microaerophilic atmosphere for growth, and their growth was stimulated by formate and fumarate. these strains were biochemically similar to campylobacter curvus and campylobacter rectus, but were clearly distinguishable from ... | 1993 | 7694633 |
| campylobacter rectus in human periodontitis. | campylobacter rectus (formerly wolinella recta) in periodontitis lesions was studied relative to age and sex distribution, relationship to disease-active periodontitis, response to periodontal debridement and in vitro antimicrobial susceptibility. subgingival c. rectus was collected with paper points, transported in vmga iii and plated onto nonselective enriched brucella blood agar and hammond's selective medium for c. rectus, both incubated anaerobically. c. rectus was recovered from 80% of 165 ... | 1993 | 8247610 |
| development and characterization of porphyromonas gingivalis-specific rat t-cell clones. | porphyromonas gingivalis has been implicated as a major pathogen in periodontitis. to determine the role of t cells in the regulation of this disease, a method was developed for the generation and characterization of rat t-cell clones with antigen specificity to p. gingivalis whole cells. the clones studied so far demonstrated a t-helper (th) phenotype w3/13+, w3/25+, ox8- and ox22-. these t-cell clones proliferated in vitro in response to p. gingivalis, but not to other bacteria (prevotella int ... | 1992 | 1281976 |
| analysis of cell surface antigens of campylobacter rectus. | campylobacter rectus is one of the predominant bacteria in the lesions of human periodontitis. the surface antigens of the bacterium contain several components which may play significant roles in colonization and pathogenesis. a high-molecular-weight protein was selectively isolated from the cell surface of c. rectus by acid extraction and purified by deae sepharose. sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis showed that the extracted protein was 150 kda. the protein was ... | 1992 | 1303324 |
| microbiology of healthy and diseased periodontal sites in poorly controlled insulin dependent diabetics. | a group of poorly-controlled insulin dependent diabetes mellitus (iddm) patients were examined in a cross-sectional design for total microbial levels, microbial incidence, and the percent levels of selected periodontal microorganisms. these organisms were selected on the basis of prior reports that associated them with either periodontal disease or health. one periodontally-healthy and one periodontally-diseased site were examined in each iddm patient. increased levels of the periodontal pathoge ... | 1992 | 1315389 |
| humoral igg antibodies to oral microbiota in a population at risk for root-surface caries. | mutans streptococci have been strongly implicated in the initiation of dental caries on coronal surfaces. their role in development of root-surface caries is less clear. the etiologic agents of both types of dental caries are likely to elicit systemic immune responses. the objective of the present study, therefore, was to study the association of clinical variables of disease with humoral igg antibodies to nine oral micro-organisms in 314 adult subjects, aged 45-65 years, who were at risk for ro ... | 1992 | 1321176 |
| e-test: a new technique for antimicrobial susceptibility testing for periodontal microorganisms. | the purpose of the study was to validate the epsilometer test (e-test) method for antimicrobial susceptibility testing of selected periodontopathic microorganisms using the agar dilution method as a standard. the e-test has been developed to provide a direct quantification of antimicrobial susceptibility of microorganisms. the device consists of a predefined, continuous, and exponential gradient of antibiotic concentrations immobilized along a rectangular plastic test strip. after 48 hours incub ... | 1992 | 1324301 |
| synergistic effect of pyrophosphate and sodium dodecyl sulfate on periodontal pathogens. | our previous studies have shown that pyrophosphate (ppi), the anticalculus component of tartar-control dentifrices, inhibits the growth of organisms associated with coronal and root surface caries. the purposes of this investigation were to: 1) determine if periodontal pathogens are similarly susceptible to the growth-inhibitory properties of ppi; and 2) determine if combinations of pyrophosphate-sodium dodecyl sulfate (ppi-sds) inhibit growth synergistically. porphyromonas gingivalis, actinobac ... | 1992 | 1324304 |
| microbial etiology of periodontal diseases. where are we? where are we going? | many studies indicate a strong association of actinobacillus actinomycetemcomitans with localized juvenile periodontitis. species associated with adult periodontitis include bacteroides forsythus, porphyromonas gingivalis, prevotella intermedia, a. actinomycetemcomitans, and wolinella recta. capnocytophaga species may be important in pubertal gingivitis. an unnamed spirochete related to treponema pallidum has been identified in acute necrotizing ulcerative gingivitis lesions. species isolated fr ... | 1992 | 1325846 |
| cytochrome composition and oxygen-dependent respiration-driven proton translocation in wolinella curva, wolinella recta, bacteroides ureolyticus, and bacteroides gracilis. | the membrane fractions of the microaerobically grown type strains of wolinella curva, wolinella recta, bacteroides ureolyticus, and bacteroides gracilis contained membrane-bound cytochrome b, cytochrome c, and co-binding cytochrome c. soluble cytochrome c and co-binding cytochrome c were also present. although b. gracilis is oxidase negative, it possessed cytochrome c. with h2 or formate as the electron donor, proton efflux from anaerobic cells occurred upon addition of a pulse of oxygen. with f ... | 1992 | 1325865 |
| putative periodontopathogens in "diseased" and "non-diseased" persons exhibiting poor oral hygiene. | the aim of the study was to assess the occurrence of some putative periodonto-pathogens in "test" and "control" sites in "diseased" and "non-diseased" persons, respectively, from an adult rural kenyan population exhibiting poor oral hygiene and widespread loss of attachment (la). 14 persons (less than 35 years) were assigned to a "diseased" category on the basis of at least 4 sites with la greater than or equal to 4 mm; at least 5 mm la and a pocket greater than or equal to 4 mm interproximally ... | 1992 | 1346265 |
| pore-forming ability of major outer membrane proteins from wolinella recta atcc 33238. | three major outer membrane proteins with apparent molecular masses of 43, 45, and 51 kda were purified from wolinella recta atcc 33238, and their pore-forming abilities were determined by the black lipid bilayer method. the non-heat-modifiable 45-kda protein (omp 45) showed no pore-forming activity even at high kcl concentrations. the single-channel conductances in 1 m kcl of the heat-modifiable proteins with apparent molecular masses of 43 kda (omp 43) and 51 kda (omp 51) were 0.49 and 0.60 ns, ... | 1992 | 1370429 |
| specific detection of campylobacter jejuni and campylobacter coli by using polymerase chain reaction. | development of a routine detection assay for campylobacter jejuni and campylobacter coli in clinical specimens was undertaken by using the polymerase chain reaction (pcr). an oligonucleotide primer pair from a conserved 5' region of the flaa gene of c. coli vc167 was used to amplify a 450-bp region by pcr. the primer pair specifically detected 4 strains of c. coli and 47 strains of c. jejuni; but it did not detect strains of campylobacter fetus, campylobacter lari, campylobacter upsaliensis, cam ... | 1992 | 1400961 |
| susceptibility of anaerobic microorganisms to hypothiocyanite produced by lactoperoxidase. | the susceptibility of capnocytophaga ochracea, eikenella corrodens, eubacterium yurii, fusobacterium nucleatum, peptostreptococcus micros, prevotella intermedia, selenomonas sputigena, wolinella recta to hypothiocyanite (oscn-) produced by the lactoperoxidase system was tested. results showed a decrease of bacterial survival rate after oscn- exposure, with an intra- and inter-species variability from 0 to 95% for c. ochracea, 34-100% for e. corrodens, 0-83% for e. yurii, 1-15% for f. nucleatum, ... | 1992 | 1481764 |
| associations between microbial species in dental root canal infections. | the existence of commensal or antagonistic relationships between microorganisms in the root canals of teeth with apical periodontitis was investigated. samples were taken from 65 infected human root canals and were analysed according to species, frequency of occurrence and proportion of the total isolated flora. the most frequent species were fusobacterium nucleatum, prevotella intermedia, peptostreptococcus micros, peptostreptococcus anaerobius, eubacterium alactolyticum, eubacterium lentum and ... | 1992 | 1494447 |
| wolinella recta in adult gingivitis and periodontitis. | wolinella recta has been associated with adult periodontitis, but its role in the disease remains uncertain. this report clarifies the distribution of w. recta in periodontally healthy and diseased subjects, and treated patients with recurrent disease. a specific polyclonal rabbit antiserum against w. recta strain 372 was used for indirect immunofluorescence localization of w. recta in dental plaque from untreated and treated patients. supragingival plaque was collected from 15 periodontally hea ... | 1992 | 1531512 |
| production of an extracellular toxin by the oral pathogen campylobacter rectus. | the atcc type strain and six clinical isolates of campylobacter rectus were tested for toxicity against hl-60 cells and human polymorphonuclear neutrophils (pmns). after challenge with bacterial cell suspensions and media supernatants for up to 4 h, eukaryotic cell viability was assayed by trypan blue dye exclusion and lactate dehydrogenase release. cells of the c. rectus type strain were not toxic. however, ethanol and (nh4)2so4 extracts of culture media supernatants killed hl-60 cells in a tim ... | 1992 | 1560755 |
| revision of campylobacter, helicobacter, and wolinella taxonomy: emendation of generic descriptions and proposal of arcobacter gen. nov. | hybridization experiments were carried out between dnas from more than 70 strains of campylobacter spp. and related taxa and either 3h-labeled 23s rrnas from reference strains belonging to campylobacter fetus, campylobacter concisus, campylobacter sputorum, campylobacter coli, and campylobacter nitrofigilis, an unnamed campylobacter sp. strain, and a wolinella succinogenes strain or 3h- or 14c-labeled 23s rrnas from 13 gram-negative reference strains. an immunotyping analysis of 130 antigens ver ... | 1991 | 1704793 |
| atypical structure of the 23s ribosomal rna molecule in certain oral bacteria. | ribosomal rna (rrna) isolated from wolinella recta and seven related bacteria was examined by agarose gel electrophoresis. the 23s rrna molecule could not be detected in w. recta, wolinella curva, bacteroides gracilis, or bacteroides ureolyticus. in place of the 23s molecule, there were three smaller molecules of approximately 1700, 650, and 600 bases designated 23s alpha, 23s beta, and 23s delta, respectively. an intact 23s rrna molecule could be isolated from wolinella succinogenes, campylobac ... | 1991 | 1710629 |
| the microflora of periodontal sites showing active destructive progression. | 20 adult periodontitis (ap) subjects were examined every 2 to 4 months and microbiological samples were collected and cultured when 2 mm or more loss of attachment (active sites) was detected by 2 examiners. similar sites in which no progressive destruction was observed (control sites) also were sampled in the same subjects. by lambda-analysis, there was no statistically significant difference in floras of active (42 sites from 12 subjects) and control (36 sites from 12 subjects) sites or betwee ... | 1991 | 1752997 |
| antimicrobial activity of flurbiprofen and ibuprofen in vitro against six common periodontal pathogens. | nonsteroidal antiinflammatory drugs (nsaids) such as flurbiprofen and ibuprofen, have been shown to inhibit the inflammation and alveolar bone loss associated with chronic destructive periodontal disease. however, the direct effect of nsaids on the gingival crevice microflora has not been studied. the purpose of this investigation was to evaluate the antimicrobial activity of ibuprofen and flurbiprofen in vitro on six commonly isolated periodontal pathogens. the bacterial strains evaluated were ... | 1991 | 1812907 |
| multicenter evaluation of tetracycline fiber therapy. iii. microbiological response. | in a multicenter study of the effects of tetracycline (tc) fiber therapy, subgingival plaque samples were tested for 6 probable periodontal pathogens by dna probe analysis. levels of actinobacillus actinomycetemcomitans, eikenella corrodens, fusobacterium nucleatum, porphyromonas (bacteroides) gingivalis, prevotella intermedia (bacteroides intermedius), and wolinella recta were quantitatively determined in samples taken at baseline, and immediately after tc fiber removal, control fiber removal, ... | 1991 | 1832457 |
| tetracycline fiber therapy monitored by dna probe and cultural methods. | oligonucleotide dna probe and selective cultural methods were compared in their ability to monitor 6 putative periodontal pathogens in a study evaluating local tetracycline fiber therapy. subgingival plaque was sampled from 4 sites in each of 20 subjects. samples were taken before and after therapy from sites assigned to the following test groups: tetracycline (tc) fiber, scaling and root planing, control fiber, and untreated. each sample was analyzed by both dna probe and cultural methods. tota ... | 1991 | 1832458 |
| virulence of wolinella recta in a murine abscess model. | the virulence of wolinella recta isolates was studied in an experimental animal model by using monoinfection of balb/c mice. infection with clinical isolates of w. recta 576 and w. recta 234 induced dry, flat, depressed gangrenous necrotic skin lesions, whereas w. recta atcc 33238 failed to induce a similar lesion. histological examination of the skin lesion 72 h postinfection revealed coagulation necrosis of the epidermis, subcutis and cutaneous truncus muscle, with marked exudation of serum pr ... | 1991 | 1855996 |
| dental bacterial plaques. nature and role in periodontal disease. | antony van leeuwenhoek first described oral bacteria. however, not until almost 200 years later was the famous koch postulate introduced. since then, research has extensively been performed regarding the development and microbiology of dental plaques. in spite of the complexity of the developing flora of supragingival plaque, culture studies have shown a remarkably orderly succession of organisms. lately, the concept of microbial specificity in the etiology of periodontal diseases has been widel ... | 1991 | 1890226 |
| microbial aspects of failing osseointegrated dental implants in humans. | the subgingival microflora of 18 failing implants were examined for pathogenic periodontal microorganisms. peptostreptococcus micros was recovered from 6 failing implants, wolinella recta from 6, fusobacterium species from 5, candida albicans from 5, and bacteroides intermedius from 4. enteric rods or pseudomonads constituted a significant part of the microflora in 5 failing implants. actinobacillus actinomycetemcomitans, nonpigmented bacteroides species, capnocytophage species, and staphylococc ... | 1991 | 1906537 |
| comparative study of lipopolysaccharides from wolinella recta, w. curva, w. succinogenes and campylobacter sputorum ssp. sputorum. | lipopolysaccharides (lps) were extracted from cells of wolinella recta atcc 33238, w. curva atcc 33224, w. succinogenes atcc 29543 and campylobacter sputorum ssp. sputorum a 3563 by a hot phenol-water method and purified by nuclease treatment and by repeated ultracentrifugation. chemical compositions of the purified lps including fatty acid and sugar composition were examined and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) was performed. all lps preparations contained a ... | 1991 | 1916228 |
| monoclonal antibody to a specific antigen from wolinella recta atcc 33238. | four monoclonal antibodies (m-abs) to a specific antigen (150-kda protein antigen) isolated from wolinella recta atcc 33238 by acid extraction were obtained. the four antibodies were all of the igg1 subclass and exhibited equally high specificity for the antigen. a battery of 14 strains or oral bacteria were screened for cross-reactivity with each mab by elisa and western blot analysis. weak cross-reactivity to some strains was observed which differed depending upon the mab. immuno-electromicros ... | 1991 | 1936935 |
| bacteroides ureolyticus (ntu) medium for the selective recovery of bacteroides gracilis. | bacteroides gracilis is a gram-negative anaerobic bacillus which requires formate and fumarate for growth; it has been implicated in periodontal disease and serious infections of the head and neck. in this study, bacteroides ureolyticus (ntu) medium was tested for its ability to allow the growth of b. gracilis and other formate-fumarate requiring gram-negative anaerobes and to enable the recovery of these organisms from clinical specimens. all reference strains grew on ntu medium with the except ... | 1991 | 1942000 |
| analysis of in vitro lymphoproliferative responses and antibody formation following subcutaneous injection of actinobacillus actinomycetemcomitans and wolinella recta in a murine model. | the potential of wolinella recta and actinobacillus actinomycetemcomitans to cause abscesses and induce an immune response was tested in balb/c mice. mice were injected subcutaneously with w. recta, a. actinomycetemcomitans or a mixture of these 2 microorganisms. mice injected with a. actinomycetemcomitans alone, or with both organisms, demonstrated abscesses at the injection site 2 days later, from which pure cultures of a. actinomycetemcomitans were isolated. mice injected with w. recta had sm ... | 1991 | 1945478 |
| dna probe detection of periodontal pathogens in hiv-associated periodontal lesions. | recent studies have shown that an atypical gingivitis and a rapidly progressive periodontal disease may be early-occurring opportunistic infections associated with human immunodeficiency virus (hiv) infection. this study examined the prevalence of selected periodontal pathogens associated with these hiv-related periodontal lesions. subgingival plaque samples were obtained from both hiv-seronegative and hiv-seropositive homosexual men and from presumably uninfected heterosexual men. dna probes we ... | 1991 | 1945481 |
| aspartate and asparagine as electron acceptors for wolinella recta. | since fumarate and nitrate are not usually available in the oral ecosystem, it was investigated whether aspartate and asparagine could be used as alternative electron acceptors by wolinella recta, which is strictly dependent on a respiratory metabolism with formate or h2 as electron donors. both aspartate and asparagine were indeed shown to support growth of w. recta with formate as electron donor. fermentative growth with aspartate alone was not possible. succinate was the major end-product and ... | 1991 | 1945491 |
| dna probe detection of eikenella corrodens, wolinella recta and fusobacterium nucleatum in subgingival plaque. | this cross-sectional study used species-specific dna probes to examine subgingival plaque specimens for the presence of eikenella corrodens, wolinella recta, and fusobacterium nucleatum in adults with untreated periodontitis or gingivitis and in healthy controls. w. recta and f. nucleatum were more prevalent in diseased sites from the periodontitis group when compared with the controls (81% vs 22% and 83% vs 20% respectively). e. corrodens was detected in 62% of the control sites and 81% of the ... | 1991 | 1945492 |
| multivariate analyses of cellular fatty acids in bacteroides, prevotella, porphyromonas, wolinella, and campylobacter spp. | the genera bacteroides, wolinella, and campylobacter contain several similar species that require taxonomic revision. fatty acid profiles of whole bacterial cells have proven useful for taxonomy. in this study, cellular fatty acids from bacteroides, prevotella, porphyromonas, wolinella, and campylobacter spp. were identified and quantitated by gas chromatography and gas chromatography-mass spectrometry, and the data were subjected to principal component analyses. bacteroides fragilis, the type s ... | 1991 | 1993755 |
| microbiological study of hiv-related periodontitis. | the subgingival microbiota in 14 persons with hiv-periodontitis was examined. subgingival plaque samples were collected with paper points, transported in vmga iii, and plated on anaerobic enriched brucella blood agar and various selective media. hiv-periodontitis sites revealed actinobacillus actinomycetemcomitans, wolinella recta, peptostreptococcus micros, and bacteroides intermedius, each averaging 7% to 16% of the cultivable subgingival flora in positive patients. high levels of spirochetes ... | 1991 | 2002434 |
| extraction, purification, and characterization of major outer membrane proteins from wolinella recta atcc 33238. | the outer membrane of wolinella recta atcc 33238 was isolated by french pressure cell disruption and differential centrifugation. outer membrane proteins (omps) were solubilized by zwittergent 3.14 extraction and separated by deae-sephacel ion-exchange chromatography. the major omps that were found in w. recta atcc 33238 and in several other wolinella spp. consisted of proteins with apparent molecular masses of 51, 45, and 43 kda. these three conserved proteins were purified to essential homogen ... | 1991 | 1894372 |
| wolinella recta, wolinella curva, bacteroides ureolyticus, and bacteroides gracilis are microaerophiles, not anaerobes. | although the nonfermentative, asaccharolytic, putative anaerobes wolinella curva, wolinella recta, bacteroides ureolyticus, and bacteroides gracilis are phylogenetically related to the true campylobacters, the type strains of these species exhibited o2-dependent microaerophilic growth in brucella broth and on brucella agar. the optimum o2 levels for growth of these strains ranged from 4 to 14% in brucella broth and from 2 to 8% on brucella agar, when h2 was provided as the electron donor. no gro ... | 1991 | 1854636 |
| surface characteristics of wolinella recta atcc 33238 and human clinical isolates: correlation of structure with function. | selected characteristics of the surface of wolinella recta atcc 33238 and three w. recta clinical isolates (ci) were studied as well as the adherence of these strains to human gingival fibroblasts (hgf). w. recta atcc 33238 and the ci were examined by electron microscopy, electrophoresis, isoelectric focusing, and adherence to hgf. electron microscopic examination of ci revealed the presence of a periodic paracrystalline layer external to and associated with the outer membrane. this surface laye ... | 1990 | 2387623 |
| hydrophobicities of human polymorphonuclear leukocytes and oral bacteroides and porphyromonas spp., wolinella recta, and eubacterium yurii with special reference to bacterial surface structures. | the hydrophobicities of human polymorphonuclear leukocytes (pmnls) and bacteroides buccae, b. oris, b. oralis, b. veroralis, b. buccalis, b. heparinolyticus, b. intermedius, b. denticola, b. loescheii, b. melaninogenicus, porphyromonas gingivalis, p. endodontalis, wolinella recta, and eubacterium yurii were studied by the hexadecane method. the majority of the strains were equally or less hydrophobic than the pmnls. only in the case of e. yurii and the only strain of b. buccalis were all strains ... | 1990 | 2091243 |
| three-dimensional structure of the surface layer of wolinella recta. | the three-dimensional structure of the crystalline surface layer (s-layer) of wolinella recta atcc 33238t, a gram-negative, anaerobic periodontopathogen, was determined to 3.8 nm resolution by electron microscopy and digital image processing. the s-layer protein is closely associated with the outer bacterial membrane, and shows p6 symmetry with lattice spacing and thickness of 21 nm and 15 nm, respectively. the funnel-shaped subunits consist of 6 heavy domains located round a common base at the ... | 1990 | 2080071 |
| effect of a triclosan/copolymer/fluoride dentifrice on the oral microflora. | eighty-one human subjects completed a double-blind study which examined the effects of a 0.3% triclosan/2% gantrez copolymer/0.243% sodium fluoride dentifrice on the microflora of supragingival dental plaque. subjects were divided into an experimental group which performed normal oral hygiene with the triclosan/copolymer/fluoride dentifrice and a control group which also performed normal oral hygiene with the same dentifrice minus the triclosan/copolymer. at baseline, 10 weeks, and 28 weeks, sup ... | 1990 | 2083042 |
| monoclonal antibodies for the detection of 'periodontopathic' bacteria. | the development of indirect immunofluorescence assays using mabs as specific probes has made it possible to detect a variety of suspected periodontal pathogens in subgingival plaque, with exquisite sensitivity. the studies demonstrate high reproducibility of the results if different mabs are used to assess the same bacterial species. bacteroides forsythus, bact. gingivalis, bact. intermedius and wolinella recta, but not actinobacillus actinomycetemcomitans were found in sites of adult periodonti ... | 1990 | 2088218 |
| effect of actinobacillus actinomycetemcomitans, wolinella recta and bacteroides gingivalis on the viability of retinoic acid-induced and dimethyl sulfoxide-induced hl-60 cells. | we studied the interactions between viable and heat-killed, opsonized and unopsonized periodontopathic bacteria with both uninduced and induced hl-60 promyelocytic leukemia cells. the cells were induced to differentiate into granulocyte-like cells by incubation with retinoic acid (ra) or dimethyl sulfoxide (dmso). when unopsonized, wolinella recta atcc 33228 significantly suppressed the net proliferation of uninduced hl-60 cells, actinobacillus actinomycetemcomitans strain y4 was markedly lethal ... | 1990 | 2098697 |
| interaction of gram-negative periodontal pathogens with retinoic acid-induced and dimethyl sulfoxide-induced hl-60 cells. | as a first step toward elucidating the reasons for differences among periodontal pathogens in their cytotoxic effects on hl-60 cells, we used transmission electron microscopy to examine morphological aspects of granulocyte-bacteria interactions. unopsonized actinobacillus actinomycetemcomitans strain y4 and bacteroides gingivalis atcc 33277 adhered to, and were phagocytosed by, retinoic acid-induced and dimethyl sulfoxide-induced hl-60 cells. in contrast, there was only minimal interaction betwe ... | 1990 | 2098698 |
| sialidase (neuraminidase) activity among gram-negative anaerobic and capnophilic bacteria. | a filter paper spot test with 2'-(4-methylumbelliferyl)-alpha-d-n-acetylneuraminic acid as a substrate was used to study the prevalence of sialidase activity among gram-negative anaerobic and capnophilic bacteria. a total of 567 isolates representing four genera of obligate anaerobes and four genera of capnophilic organisms was tested. sialidase activity was detected in 94% of 66 isolates from the bacteroides fragilis group, 98% of 66 b. bivius isolates, and all isolates of the following species ... | 1990 | 2108991 |
| studies of the subgingival microflora in patients with acquired immunodeficiency syndrome. | two unique forms of periodontal disease, hiv-gingivitis and hiv-periodontitis, have been described in patients with acquired immunodeficiency syndrome (aids). in order to determine the bacterial species associated with periodontitis in aids patients, the predominant cultivable microflora was examined in 21 subgingival plaque samples from 11 aids patients with periodontitis. the presence of putative periodontal pathogens including actinobacillus actinomycetemcomitans, bacteroides intermedius, por ... | 1990 | 2123926 |
| susceptibility of oral bacteria to phenoxyethanol and phenoxyethanol/chlorhexidine combinations. | a total of 57 bacterial strains (26 different species) which may be isolated from subgingival plaque were tested for their in vitro susceptibility to phenoxycthanol, a commonly-used antiseptic and preservative. ninety-five percent of the strains, including those associated with chronic inflammatory periodontal disease, were susceptible to concentrations of phenoxyethanol used topically (2% w/v). phenoxyethanol at a concentration of 1% (w/v) was also found that to have a rapid bactericidal effect ... | 1990 | 2202809 |