Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
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| fibulin-1 binds the amino-terminal head of beta-amyloid precursor protein and modulates its physiological function. | genetic studies have implicated amyloid precursor protein (app) in the pathogenesis of alzheimer's disease. while accumulating lines of evidence indicate that app has various functions in cells, little is known about the proteins that modulate its biological activity. toward this end, we employed a two-hybrid system to identify potential interacting factors. we now report that fibulin-1, which contains repetitive ca(2+)-binding egf-like elements, binds to app at its amino-terminal growth factor- ... | 2001 | 11238726 |
| characterization of mouse brain-specific angiogenesis inhibitor 1 (bai1) and phytanoyl-coa alpha-hydroxylase-associated protein 1, a novel bai1-binding protein. | previously, pahx-ap1 (pahx-associated protein 1) was isolated as a novel protein to interact with refsum disease gene product (phytanoyl-coa alpha-hydroxylase, pahx) and specifically expressed in mouse brain. pahx-ap1 is also suggested to be involved in the development of the central neurologic deficits of refsum disease. to clarify its function, we have searched for proteins that associate with pahx-ap1 via yeast two-hybrid system. we found that pahx-ap1 interacts with the cytoplasmic region of ... | 2001 | 11245925 |
| sequence analysis of the aminoacylase-1 family. a new proposed signature for metalloexopeptidases. | the amino acid sequence analysis of the human and porcine aminoacylases-1, the carboxypeptidase s precursor from saccharomyces cerevisiae, the succinyl-diaminopimelate desuccinylase from escherichia coli, haemophilus influenzae and corynebacterium glutamicum, the acetylornithine deacetylase from escherichia coli and dictyostelium discoideum and the carboxypeptidase g(2) precursor from pseudomonas strain, using the basic local alignment search tool (blast) and the position-specific iterated blast ... | 2001 | 11250542 |
| interactions of surfactant proteins a and d with saccharomyces cerevisiae and aspergillus fumigatus. | surfactant proteins a (sp-a) and d (sp-d) are members of the collectin family of calcium-dependent lectins and are important pulmonary host defense molecules. human sp-a and sp-d and rat sp-d bind to aspergillus fumigatus conidia, but the ligand remains unidentified. to identify a fungal ligand for sp-a and/or sp-d, we examined the interactions of the proteins with saccharomyces cerevisiae. sp-d but not sp-a bound yeast cells, and edta inhibited the binding. sp-d also aggregated yeast cells and ... | 2001 | 11254556 |
| intracellular crystal formation as a mechanism of cytotoxicity in murine pulmonary cryptococcus neoformans infection. | rod-like crystalline structures formed during eosinophilic cryptococcus neoformans pneumonia in c57bl/6 mice. crystals were found associated with yeast cells and free in host cell cytoplasm. the crystals apparently formed because of the interaction of a host protein with the cryptococcal polysaccharide. crystal formation likely contributes to pathogenesis by causing cellular damage. | 2001 | 11254641 |
| d-mannoheptulose phosphorylation by hexokinase isoenzymes. | d-mannoheptulose is a specific inhibitor of d-glucose phosphorylation by hexokinase isoenzymes. in the present study, the phosphorylation of this heptose was investigated by either a spectrophotometric or radioisotopic procedure. using yeast hexokinase, the phosphorylation of 25 mm d-mannoheptulose only represented 0.02% of that of 5 mm d-glucose. such a percentage was increased to 3.93% in the case of bovine heart hexokinase. in the latter case, the km for d-mannoheptulose was close to 0.2 mm a ... | 2001 | 11254873 |
| safety evaluation of phosphodiesterase produced from penicillium citrinum: summary of toxicological data. | the toxicity of enzyme rp-1, an enzyme preparation used to hydrolyze yeast rna to produce flavor enhancers for use in the food industry, was evaluated in a series of studies. a 5-week dietary toxicity study in wistar rats was conducted in which animals received enzyme rp-1 in feed at concentrations of 0, 500, 2000, or 8000 mg/kg body wt/day. a 13-week dietary toxicity study in sprague-dawley rats was conducted in which animals received rp-1 concentrate at 0, 0.125, 0.5, or 2% in their diets. at ... | 2001 | 11259174 |
| comparison of the modulatory effects of human and rat liver microsomal metabolism on the estrogenicity of bisphenol a: implications for extrapolation to humans. | bisphenol a [bpa, 2,2-bis(4-hydroxyphenyl)propane], a xenoestrogen, is a monomer for the synthesis of polycarbonate plastics, epoxy resins, and composites. metabolism of bpa to the monoglucuronide will determine the extent of its estrogenicity in vivo. investigation of the metabolism of bpa (500 microm) by isolated female rat hepatocytes confirmed the formation of bpa glucuronide as the major metabolite. there was a significant difference (p < 0.05) between the v(max) (mean +/- s.e.m., n = 4) of ... | 2001 | 11259533 |
| synthesis and src kinase inhibitory activity of a series of 4-phenylamino-3-quinolinecarbonitriles. | screening of a directed compound library in a yeast-based assay identified 4-[(2,4-dichlorophenyl)amino]-6,7-dimethoxy-3-quinolinecarbonitrile (2a) as a src inhibitor. an enzymatic assay established that 2a was an atp-competitive inhibitor of the kinase activity of src. we present here sar data for 2a which shows that the aniline group at c-4, the carbonitrile group at c-3, and the alkoxy groups at c-6 and c-7 of the quinoline are crucial for optimal activity. increasing the size of the c-2 subs ... | 2001 | 11262092 |
| expression of rat liver long-chain acyl-coa synthetase and characterization of its role in the metabolism of r-ibuprofen and other fatty acid-like xenobiotics. | our investigations of fatty acid metabolism and epimerization of the 2-arylpropionic acid derivative, r-ibuprofen, resulted in the successful purification of an acyl-coa synthetase from rat liver microsomes that catalyzes the formation of both palmitoyl-coa and r-ibuprofenoyl-coa. to investigate whether r-ibuprofenoyl-coa synthetase and long-chain acyl-coa synthetase (lacs) are identical enzymes, we cloned the cdna from lacs into the pqe30 expression vector and transformed the construct into esc ... | 2001 | 11266649 |
| isolation of sporothrix schenckii from the nails of domestic cats (felis catus). | we report the first isolation of sporothrix schenckii from the nail surfaces of cats. the fungus grew from nail clippings of three cats associated with three household outbreaks of sporotrichosis involving cats and human beings. the identification of the isolates was based on macroscopic and microscopic morphological characteristics at 25 degrees c and conversion of s. schenckii to the yeast-like form at 37 degrees c. | 2001 | 11270404 |
| the human tumor suppressor arf interacts with spinophilin/neurabin ii, a type 1 protein-phosphatase-binding protein. | the ink4a gene, one of the most often disrupted loci in human cancer, encodes two unrelated proteins, p16(ink4a) and p14(arf) (arf) both capable of inducing cell cycle arrest. although it has been clearly demonstrated that arf inhibits cell cycle via p53 stabilization, very little is known about the involvement of arf in other cell cycle regulatory pathways, as well as on the mechanisms responsible for activating arf following oncoproliferative stimuli. in search of factors that might associate ... | 2001 | 11278317 |
| a store-operated nonselective cation channel in lymphocytes is activated directly by ca(2+) influx factor and diacylglycerol. | agonist-receptor interactions at the plasma membrane often lead to activation of store-operated channels (socs) in the plasma membrane, allowing for sustained ca(2+) influx. while ca(2+) influx is important for many biological processes, little is known about the types of socs, the nature of the depletion signal, or how the socs are activated. we recently showed that in addition to the ca(2+) release-activated ca(2+) (crac) channel, both jurkat t cells and human peripheral blood mononuclear cell ... | 2001 | 11287342 |
| conservation of the morf4 related gene family: identification of a new chromo domain subfamily and novel protein motif. | the seven member, human morf4 related gene (mrg) family was recently identified based on the ability of mortality factor on chromosome 4 (morf4) to induce replicative senescence in immortal cell lines assigned to complementation group b (bertram et al., 1999. mol. cell biol. 19, 1479-1485). initial computer based similarity searches identified human retinoblastoma binding protein 1 (rbp-1), drosophila melanogaster male specific lethal-3 (msl-3), s. pombe altered polarity-13 (alp13) and s. cerevi ... | 2001 | 11290425 |
| validating clustering for gene expression data. | motivation: many clustering algorithms have been proposed for the analysis of gene expression data, but little guidance is available to help choose among them. we provide a systematic framework for assessing the results of clustering algorithms. clustering algorithms attempt to partition the genes into groups exhibiting similar patterns of variation in expression level. our methodology is to apply a clustering algorithm to the data from all but one experimental condition. the remaining condition ... | 2001 | 11301299 |
| menin, a gene product responsible for multiple endocrine neoplasia type 1, interacts with the putative tumor metastasis suppressor nm23. | although the gene responsible for multiple endocrine neoplasia type 1 (men1) has been identified, the function of its gene product, menin, is unknown. to examine the biological role of the men1 gene, we searched for associated proteins with a yeast two-hybrid system using the men1 cdna fragment as bait. on screening a rat fetal brain embryonic day 17 library, in which a high level of men1 expression was detected, we identified a putative tumor metastasis suppressor nm23/nucleoside diphosphate (n ... | 2001 | 11302744 |
| cloning, organisation, chromosomal localization and expression analysis of the mouse prkag1 gene. | the mammalian 5'-amp-activated protein kinase (ampk) is a heterotrimeric protein consisting of alpha-, beta- and gamma-subunits. the alpha-subunit is the catalytic subunit. the non-catalytic subunits ampk-beta and ampk-gamma form, together with the catalytic ampk-alpha, the active kinase complex in mammals and its homologue in yeast. the gene for ampk-gamma-1 has been designated recently as prkag1. we have isolated mouse prkag1 cdna from testis (1623 nt) coding for 330 aa and we have shown its u ... | 2001 | 11306812 |
| recognition and oxidative metabolism of cyclodipeptides by hepatic cytochrome p450. | possible recognition of peptide derivatives by hepatic cytochrome p450 3a has been suggested by binding and metabolism of numerous pseudopeptidic compounds such as ergot derivatives and cyclosporin. natural linear or cyclic dipeptides containing hydrophobic amino acids produced by microorganisms and present in mammals are able to interact with the p450 active site through either iron-amine interactions (type ii) or hydrophobic type i interactions. p450 3a from dexamethasone-treated rats or yeast ... | 2001 | 11311724 |
| dna-damaging carcinogen 3-amino-1,4-dimethyl-5h-pyrido[4,3-b]indole (trp-p-1) induces apoptosis via caspase-9 in primary cultured rat hepatocytes. | the mechanism of cytotoxicity induced by the dna-damaging carcinogen 3-amino-1,4-dimethyl-5h-pyrido[4,3-b] indole (trp-p-1) was investigated in primary cultured rat hepatocytes. cytotoxicity was caused by intact trp-p-1 and not by metabolically activated derivatives prepared using a recombinant yeast strain ah22/pamr2 expressing rat cytochrome p450 1a1, and not by metabolically activated derivatives. we also found internucleosomal dna fragmentation 6 h after treatment with 30 microm trp-p-1, ind ... | 2001 | 11323386 |
| mrp2-deficiency in the rat impairs biliary and intestinal excretion and influences metabolism and disposition of the food-derived carcinogen 2-amino-1-methyl-6-phenylimidazo. | while metabolism of 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (phip), the most abundant food-derived heterocyclic amine and carcinogen, has been studied extensively in several species, transport of this compound and its metabolites has not been defined yet. therefore we studied metabolism and disposition of phip in wistar and mrp2-deficient tr(-) rats to determine the role of mrp2 in the defence against this compound. in the first 2 h after intravenous dosing, total excretion of phip and i ... | 2001 | 11323401 |
| theoretical evidence of a salt bridge disruption as the initiating process for the alpha1d-adrenergic receptor activation: a molecular dynamics and docking study. | this study reports the building of the three-dimensional structure of the rat alpha1d-adrenergic receptor through a topology approach based on the structure of the rhodopsin receptor from cryoelectron microscopy. the validity and reliability of the receptor model were assessed through exhaustive molecular dynamics and docking studies. some interesting ligand-receptor interactions were identified along with significant differences between the binding mode of agonists and antagonists. the importan ... | 2001 | 11340655 |
| vascular injury induces posttranscriptional regulation of the id3 gene: cloning of a novel id3 isoform expressed during vascular lesion formation in rat and human atherosclerosis. | the molecular mechanisms that regulate the proliferation of smooth muscle cells (smcs) of the vasculature in response to injury are poorly understood. members of the inhibitor of dna binding (id) class of helix-loop-helix transcription factors are known to regulate the growth of a variety of cell types; however, the expression of the various id genes in smcs and in vascular lesions has not been examined. in the present study, the yeast 2-hybrid system was used to clone id genes from a cultured r ... | 2001 | 11348870 |
| characterization of a novel proapoptotic caspase-2- and caspase-9-binding protein. | caspases play important roles in regulating apoptotic signaling pathways. here we report the cloning, by the yeast two hybrid system with dominant negative caspase-2 as "bait," of a proapoptotic molecule named proapoptotic caspase adaptor protein (pacap), encoded by a 372-base pair open reading frame. binding of this novel protein to caspase-2 (casp-2) was confirmed in yeast two hybrid, in vitro, and in vivo assays. the deduced amino acid sequence revealed homology to functional motifs, includin ... | 2001 | 11350957 |
| interaction between gaba(a) receptor beta subunits and the multifunctional protein gc1q-r. | gamma-aminobutyric acid type a (gaba(a)) receptors were immunopurified from bovine brain using a monoclonal antibody directed against the alpha1 subunit. of the several proteins that copurified, a 34-kda protein was analyzed further. after enrichment and tryptic proteolysis, the resulting fragments were sequenced, and the protein was identified as gc1q-r. using anti-gc1q-r and anti-gaba(a) receptor antibodies, mutual coimmunoprecipitation could be demonstrated from solubilized rat brain membrane ... | 2001 | 11350968 |
| malassezin--a novel agonist of the arylhydrocarbon receptor from the yeast malassezia furfur. | the yeast malassezia furfur converts tryptophan into several indole compounds. one of these, malassezin, was identified as 2-(1h-indol-3-ylmethyl)-1h-indole-3-carbaldehyde (1). it was synthesized from n-boc-indole-3-carbaldehyde in five steps with 12% overall yield. the compound easily cyclizes to indolo[3,2-b]carbazole (7) which is known to interact with the arylhydrocarbon receptor (ahr). similarly, malassezin was found to induce cytochrome p450 as an agonist of ahr (ec50 = 1.57 microm) in rat ... | 2001 | 11354679 |
| ucs15a, a non-kinase inhibitor of src signal transduction. | src tyrosine kinase plays key roles in signal transduction following growth factor stimulation and integrin-mediated cell-substrate adhesion. since src-signal transduction defects are implicated in a multitude of human diseases, we have sought to develop new ways to identify small molecule inhibitors using a yeast-based, activated-src over-expression system. in the present study, we describe the identification of a unique src-signal transduction inhibitor, ucs15a. ucs15a was found to inhibit the ... | 2001 | 11360191 |
| role of sp1 and sp3 in the transcriptional regulation of the fatty acid synthase gene. | inspection of the 5' region of the sequence of the rat fatty acid synthase (fas) gene revealed a high gc content between -900 and +500, implying several binding sites for members of the sp1 family of transcription factors. using sl2 and h4iie cells in conjunction with fas promoter/luciferase constructs either successively deleted or containing defined deletions we characterized six gc boxes--gc-i to gc-vi--located between -557 and -83 and discovered a seventh, gc-vii, in the first intron. in vit ... | 2001 | 11368006 |
| the hemidesmosomal protein bullous pemphigoid antigen 1 and the integrin beta 4 subunit bind to erbin. molecular cloning of multiple alternative splice variants of erbin and analysis of their tissue expression. | the bullous pemphigoid antigen 1 (ebpag1) is a constituent of hemidesmosomes (hds), cell-substrate adhesion complexes in stratified epithelia. although its cooh terminus interacts with intermediate filaments, its nh(2) terminus is important for its recruitment into hds. to identify proteins that interact with the nh(2) terminus of human ebpag1, we performed a yeast two-hybrid screen, which uncovered a protein belonging to the lap/lerp (for lrr and pdz domain) protein family with 16 nh(2)-termina ... | 2001 | 11375975 |
| transport of the beta -o-glucuronide conjugate of the tobacco-specific carcinogen 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol (nnal) by the multidrug resistance protein 1 (mrp1). requirement for glutathione or a non-sulfur-containing analog. | nitrosamine 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (nnk) and its metabolite 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol (nnal) play a crucial role in the induction of lung cancer, and nnal-o-glucuronide formation and elimination are important steps in detoxification of these compounds. in the present study, we investigated the atp-binding cassette (abc) protein, mrp1 (abcc1), as a candidate transporter responsible for nnal-o-glucuronide export. mrp1 mediates the active transport of num ... | 2001 | 11375986 |
| fibroblast growth factor homologous factor 1b binds to the c terminus of the tetrodotoxin-resistant sodium channel rnav1.9a (nan). | in this study we demonstrate a direct interaction between a cytosolic fibroblast growth factor family member and a sodium channel. a yeast two-hybrid screen for proteins that associate with the cytoplasmic domains of the tetrodotoxin-resistant sodium channel rna(v)1.9a (nan) led to the identification of fibroblast growth factor homologous factor 1b (fhf1b), a member of the fibroblast growth factor family, as an interacting partner of rna(v)1.9a. fhf1b selectively interacts with the c-terminal re ... | 2001 | 11376006 |
| the anti-inflammatory, analgesic and antipyretic activity of nigella sativa. | the aqueous extract of nigella sativa (n. sativa) was investigated for anti-inflammatory, analgesic and antipyretic activities in animal models. the extract has an anti-inflammatory effect demonstrated by its inhibitory effects on carrageenan induced paw edema. it also produced significant increase in the hot plate reaction time in mice indicating analgesic effect. however, n. sativa crude suspension had no effect on yeast induced pyrexia. this study therefore, supports its use in folk medicine ... | 2001 | 11378280 |
| effects of brewer's yeast cell wall on constipation and defecation in experimentally constipated rats. | brewer's yeast cell wall (byc) was tested on constipated male sprague-dawley rats that had been induced by loperamide (2 mg/kg of body weight). the preventive effect of byc on constipation was examined and compared with that of a non-fiber diet (nf) as the control. the dose-response of byc and the effect on defecation by constipated experimental rats were also compared with the characteristics of cellulose diet (ce) group which served as a control. defecation was observed to be greater by the ra ... | 2001 | 11388452 |
| rhythmic expression of nocturnin mrna in multiple tissues of the mouse. | nocturnin was originally identified by differential display as a circadian clock regulated gene with high expression at night in photoreceptors of the african clawed frog, xenopus laevis. although encoding a novel protein, the nocturnin cdna had strong sequence similarity with a c-terminal domain of the yeast transcription factor ccr4, and with mouse and human ests. since its original identification others have cloned mouse and human homologues of nocturnin/ccr4, and we have cloned a full-length ... | 2001 | 11394964 |
| cloning, characterization and mapping of the mouse trehalase (treh) gene. | trehalase is the least studied of the membrane-bound alpha- glucosidase enzymes. here we report the isolation and characterization of the mouse trehalase (treh) gene. initially, pcr using primers based on published rat cdna sequence was used to clone a partial mouse cdna. this allowed design of mouse primers which identified a single positive clone in a bacterial artificial chromosome (bac) library of mouse genomic dna. analysis of bac subclones showed that the treh structural gene spans approxi ... | 2001 | 11404018 |
| possible androgenic/anti-androgenic activity of the insecticide fenitrothion. | to date, within the field of endocrine disruption, much focus has been placed on chemicals that mimic oestrogens (so-called xenoestrogens), and the number of such chemicals apparently detected continues to grow steadily. less effort has been expended on investigating chemicals that mimic, or antagonize, other hormones. nevertheless, a number of chemicals have been reported to have a weak affinity for the androgen receptor, all of which have, to date, been found to have anti-androgenic activity i ... | 2001 | 11404828 |
| heterogeneous rna-binding protein m4 is a receptor for carcinoembryonic antigen in kupffer cells. | here we report the isolation of the recombinant cdna clone from rat macrophages, kupffer cells (kc) that encodes a protein interacting with carcinoembryonic antigen (cea). to isolate and identify the cea receptor gene we used two approaches: screening of a kc cdna library with a specific antibody and the yeast two-hybrid system for protein interaction using as a bait the n-terminal part of the cea encoding the binding site. both techniques resulted in the identification of the rat heterogeneous ... | 2001 | 11406629 |
| purification and characterization of active recombinant rat kallikrein rk9. | the rat tissue kallikrein rk9 is most abundant in the submandibular gland and the prostate. it has been successfully expressed in the pichia pastoris yeast expression system. a full-length cdna coding for the mature rk9 was fused in frame with yeast alpha-factor cdna. the fusion protein was secreted into the medium with high yield without being processed by the yeast kex2 signal peptidase. mature rk9 was efficiently released from the fusion protein by trypsin and was purified to homogeneity by o ... | 2001 | 11410295 |
| rim1 and rabphilin-3 bind rab3-gtp by composite determinants partially related through n-terminal alpha -helix motifs. | rim1 is a protein of the presynaptic active zone, the area of the plasma membrane specialized for neurotransmitter exocytosis, and interacts with rab3, a small gtpase implicated in neurotransmitter vesicle dynamics. here, we have studied the molecular determinants of rim1 that are responsible for rab3 binding, employing surface plasmon resonance and recombinant, bacterially expressed rab3 and rim1 proteins. a site that binds gtp- but not gdp-saturated rab3 was localized to a short alpha-helical ... | 2001 | 11431472 |
| molecular and biochemical characterization of rat epsilon -n-trimethyllysine hydroxylase, the first enzyme of carnitine biosynthesis. | epsilon-n-trimethyllysine hydroxylase (ec ) is the first enzyme in the biosynthetic pathway of l-carnitine and catalyzes the formation of beta-hydroxy-n-epsilon-trimethyllysine from epsilon-n-trimethyllysine, a reaction dependent on alpha-ketoglutarate, fe(2+), and oxygen. we purified the enzyme from rat kidney and sequenced two internal peptides by quadrupole-time-of-flight mass spectroscopy. the peptide sequences were used to search the expressed sequence tag data base, which led to the identi ... | 2001 | 11431483 |
| the atm-related domain of trrap is required for histone acetyltransferase recruitment and myc-dependent oncogenesis. | the atm-related trrap protein is a component of several different histone acetyltransferase (hat) complexes but lacks the kinase activity characteristic of other atm family members. we identified a novel function for this evolutionarily conserved domain in its requirement for the assembly of a functional hat complex. ectopic expression of trrap protein with a mutation in the atm-related domain inhibits myc-mediated oncogenic transformation. the myc-binding region of trrap maps to a separable dom ... | 2001 | 11445536 |
| role of intercellular adhesion molecule 1 in acute lung injury induced by candidemia. | candidemia, a complication often affecting immunocompromised patients, is a common cause of acute lung injury. yeast-phase candida albicans has been shown to express a protein that is antigenically and structurally related to mac-1. c. albicans is reported to stimulate intercellular adhesion molecule 1 (icam-1) expression on endothelial cells. in this study, the authors examined the role of icam-1 in acute lung injury induced by candidemia. the authors cultured rat pulmonary artery endothelial c ... | 2001 | 11480583 |
| a membrane protein enriched in endoplasmic reticulum exit sites interacts with copii. | although all mammalian copii components have now been cloned, little is known of their interactions with other regulatory proteins involved in exit from the endoplasmic reticulum (er). we report here that a mammalian protein (yip1a) that is about 31% identical to s. cerevisiae and which interacts with and modulates copii-mediated er-golgi transport. yip1a transcripts are ubiquitously expressed. transcripts of a related mammalian homologue, yip1b, are found specifically in the heart. indirect imm ... | 2001 | 11489904 |
| regulation of dendritic spine morphology by spar, a psd-95-associated rapgap. | the psd-95/sap90 family of scaffold proteins organizes the postsynaptic density (psd) and regulates nmda receptor signaling at excitatory synapses. we report that spar, a rap-specific gtpase-activating protein (rapgap), interacts with the guanylate kinase-like domain of psd-95 and forms a complex with psd-95 and nmda receptors in brain. in heterologous cells, spar reorganizes the actin cytoskeleton and recruits psd-95 to f-actin. in hippocampal neurons, spar localizes to dendritic spines and cau ... | 2001 | 11502259 |
| vitronectin and fibronectin function as glucan binding proteins augmenting macrophage responses to pneumocystis carinii. | beta-glucans represent major structural components of fungal cell walls. we recently reported that pneumocystis carinii beta-glucans stimulate alveolar macrophages to release proinflammatory cytokines. macrophage activation by beta-glucan is augmented by serum, implying the presence of circulating factors that interact with beta-glucans and enhance their ability to stimulate macrophages. using beta-glucan-enriched cell wall fractions from p. carinii and saccharomyces cerevisiae, two prominent pr ... | 2001 | 11509330 |
| synaptic scaffolding proteins in rat brain. ankyrin repeats of the multidomain shank protein family interact with the cytoskeletal protein alpha-fodrin. | the postsynaptic density is the ultrastructural entity containing the neurotransmitter reception apparatus of excitatory synapses in the brain. a recently identified family of multidomain proteins termed src homology 3 domain and ankyrin repeat-containing (shank), also known as proline-rich synapse-associated protein/somatostatin receptor-interacting protein, plays a central role in organizing the subsynaptic scaffold by interacting with several synaptic proteins including the glutamate receptor ... | 2001 | 11509555 |
| analysis of small gtpase signaling pathways using p21-activated kinase mutants that selectively couple to cdc42. | p21-activated kinase 1 (pak1) is an effector for the small gtpases cdc42 and rac. because pak1 binds to and is activated by both these gtpases, it has been difficult to precisely delineate the signaling pathways that link extracellular stimuli to pak1 activation. to separate activation of pak1 by cdc42 versus activation by rac, we devised a genetic screen in yeast that enabled us to create and identify pak1 mutants that selectively couple to cdc42 but not rac1. we recovered several such pak1 mut ... | 2001 | 11514549 |
| [use of diet containing yeast protein (saccharomyces cerevisiae): effects upon pregnancy, lactation and development in rats]. | the nutritive value of manioc flour (manihot esculenta) enriched with yeast protein (saccharomyces cerevisiae) added to a food mixture most frequently consumed by low-income populations was assessed in female wistar rats (n = 30; 100-120 days old). animals were divided into three groups, mated and had free access to diets and water. diets were as follows: beans, rice, yeast-enriched manioc flour (brymf17); beans, rice, manioc flour (brmf13); casein (17% protein) (cas17). body weight gains and fo ... | 2001 | 11515236 |
| gerp95 belongs to a family of signal-transducing proteins and requires hsp90 activity for stability and golgi localization. | gerp95 (golgi-endoplasmic reticulum protein 95 kda) is part of a large family of highly conserved proteins found in all metazoans and the fission yeast schizosaccharomyces pombe. genetic studies suggest that homologs of gerp95 are components of signaling pathways that regulate cellular differentiation, development, and rna interference. however, the precise molecular functions of these proteins remain unknown. genetic analysis of gerp95 homologs has been complicated by the presence of multiple g ... | 2001 | 11553639 |
| cr16 forms a complex with n-wasp in brain and is a novel member of a conserved proline-rich actin-binding protein family. | the neuronal wiskott-aldrich syndrome protein (n-wasp) has emerged as a central regulator of the actin cytoskeleton with abilities to integrate multiple upstream signal inputs and transmit them to the arp2/3 complex. here, we demonstrate that native n-wasp is present in a tight complex with a proline-rich protein, cr16, which shares approximately 25% identity with wasp interacting protein. cr16 is encoded by a gene previously cloned as a glucocorticoid-regulated mrna from a rat hippocampal cdna ... | 2001 | 11553796 |
| terminal deoxynucleotidyltransferase is negatively regulated by direct interaction with proliferating cell nuclear antigen. | the repertoires of ig and tcr are generated by a combinatorial rearrangement of variable (v), diversity (d), and joining (j) segments (v(d)j recombination) in b- and t-cells. terminal deoxynucleotidyltransferase (tdt) adds extra nucleotides (n nucleotides) at the junctions of the gene segments to enhance the ig and tcr genes diversity. using an anti-tdt antibody column, tdt has been purified as a member of a megadalton protein complex from rat thymus. the n region would be synthesized with the l ... | 2001 | 11554927 |
| prolonged in vivo residence times of antibody fragments associated with albumin. | antibody fragments can be expressed at a high level in microbial systems, but they may have limited therapeutic value because they are rapidly eliminated from the body. we demonstrate here that site-specific conjugation or binding of bacterially derived fab' to the long-lived protein serum albumin allows full retention of the antibody's binding characteristics while imparting the albumin's longevity in vivo. in rats the area under the curve for fab' conjugated to rat serum albumin was 17-fold gr ... | 2001 | 11562193 |
| cloning and characterization of gonadotropin-inducible ovarian transcription factors (giot1 and -2) that are novel members of the (cys)(2)-(his)(2)-type zinc finger protein family. | gonadotropins are essential for ovarian follicular development and differentiation. to identify genes that are rapidly induced by gonadotropin in the immature rat ovary, ovarian genes were screened by a subtraction cloning procedure. cdna clones encoding novel members of the (cys)(2)-(his)(2)-type zinc finger protein family giot1 and -2 (gonadotropin-inducible transcription factor 1 and 2), were identified. two isoforms of giot2 (giot2 alpha and 2 beta), which are probably produced by alternativ ... | 2001 | 11579202 |
| an nmda receptor signaling complex with protein phosphatase 2a. | regulation of protein phosphatase 2a (pp2a) activity and nmda receptor (nmdar) phosphorylation state contribute to the modulation of synaptic plasticity, yet these two mechanisms have not been functionally linked. the nmdar subunit nr3a is equipped with a unique carboxyl domain that is different from other nmdar subunits. we hypothesized that the nr3a c-terminal intracellular domain might serve as synaptic anchor for the phosphatase in the developing cns. a cdna library was screened by the yeast ... | 2001 | 11588171 |
| molecular cloning and characterization of a steroid receptor-binding regulator of g-protein signaling protein cdna. | steroid hormone receptors are composed of six major functional domains, i.e. the a/b domains as the activation function 1 domain (af-1), domain c as the dna-binding domain, domain d as a hinge domain and domain e/f as the ligand-dependent transcriptional domain (af-2). they regulate gene transcription through interactions with various nuclear factors of their domains, such as af-1 and af-2. we have insufficient knowledge of the function of the dna-binding domain (domain c) except for its dna-bin ... | 2001 | 11595167 |
| histone deacetylase inhibitors arrest polyglutamine-dependent neurodegeneration in drosophila. | proteins with expanded polyglutamine repeats cause huntington's disease and other neurodegenerative diseases. transcriptional dysregulation and loss of function of transcriptional co-activator proteins have been implicated in the pathogenesis of these diseases. huntington's disease is caused by expansion of a repeated sequence of the amino acid glutamine in the abnormal protein huntingtin (htt). here we show that the polyglutamine-containing domain of htt, htt exon 1 protein (httex1p), directly ... | 2001 | 11607033 |
| lim-domain protein cysteine- and glycine-rich protein 2 (crp2) is a novel marker of hepatic stellate cells and binding partner of the protein inhibitor of activated stat1. | activation of hepatic stellate cells is considered to be the main step in the development of liver fibrosis, which is characterized by the transition of quiescent vitamin-a-rich cells to proliferative, fibrogenic and contractile myofibroblasts. the identification of regulatory genes during early cell activation and transdifferentiation is essential to extend our knowledge of hepatic fibrogenesis. in liver, the gene csrp2 is exclusively expressed by stellate cells, whereas no transcripts are dete ... | 2001 | 11672422 |
| a functional composite cis-element for nf kappa b and rbj kappa in the rat pregnancy-specific glycoprotein gene. | the rat pregnancy-specific glycoprotein gene rncgm3 is primarily expressed in the placenta. previously, three dnase i footprinting sites (fpi, fpii, and fpiii) were identified in the rncgm3 promoter region, a yeast one-hybrid screen was performed to identify the nuclear factors binding to the fpiii (5'-gcctgggaaaaaactc-3') element, and rbpj kappa, a downstream effector of the notch signaling pathway, was identified as one of the fpiii-binding factors. in the present study, the nf kappa b member ... | 2001 | 11673260 |
| coordinately regulated expression of fat/cd36 and facs1 in rat skeletal muscle. | protein-mediated fatty acid uptake and intracellular fatty acid activation are key steps in fatty acid metabolism in muscle. we have examined (a) the abundance of fatty acid translocase (fat/cd36) mrna (a fatty acid transporter) and long-chain acyl coa synthetase (facs1) mrna in metabolically heterogeneous muscles (soleus (sol), red (rg) and white gastrocnemius (wg)), and (b) whether fat/cd36 and facs1 mrnas were coordinately up-regulated in red (rta) and white tibialis muscles (wta) that had be ... | 2001 | 11681722 |
| [bmal1 and circadian rhythm]. | hbmal1 cdna has been cloned from a human brain cdna library and revealed that the cdna encoded a novel bhlh-pas transcription factor. the rbmal1 mrna expression was found not only at high levels in the suprachiasmatic nuclei (scn), but also in the pyriform cortex, hippocampus, and cerebellum. furthermore, this expression was found in the eyes, pineal body, and peripheral organs, such as the muscle, liver, and heart. bmal1 mrna levels reveal a circadian rhythm in the rat scn, with the highest exp ... | 2000 | 11326546 |
| disruption of a yeast very-long-chain acyl-coa synthetase gene simulates the cellular phenotype of x-linked adrenoleukodystrophy. | x-linked adrenoleukodystrophy (x-ald) is characterized biochemically by elevated levels of saturated very long-chain fatty acids (vlcfas) in plasma and tissues. in x-ald, peroxisomal very-long-chain acyl-coa synthetase (vlcs) fails to activate vlcfas, preventing their degradation via beta-oxidation. however, the product of the defective xald gene (aldp) is not a vlcs, but rather a peroxisomal membrane protein (pmp). disruption of either or both of two yeast pmp genes related to the xald gene did ... | 2000 | 11330068 |
| pike. a nuclear gtpase that enhances pi3kinase activity and is regulated by protein 4.1n. | while cytoplasmic pi3kinase (pi3k) is well characterized, regulation of nuclear pi3k has been obscure. a novel protein, pike (pi3kinase enhancer), interacts with nuclear pi3k to stimulate its lipid kinase activity. pike encodes a 753 amino acid nuclear gtpase. dominant-negative pike prevents the ngf enhancement of pi3k and upregulation of cyclin d1. ngf treatment also leads to pike interactions with 4.1n, which has translocated to the nucleus, fitting with the initial identification of pike base ... | 2000 | 11136977 |
| interaction of the c-terminal tail region of the metabotropic glutamate receptor 7 with the protein kinase c substrate pick1. | group iii metabotropic glutamate receptors (mglurs) are highly enriched in the presynaptic terminals of glutamatergic synapses where they mediate feedback inhibition of neurotransmitter release. here, we used the yeast two-hybrid system to identify a direct interaction of the c-terminal tail region of mglur7 with the rat homologue of the protein kinase c substrate pick1. this interaction is specifically mediated by the very c-terminal amino acids of the receptor and can be reconstituted in human ... | 2000 | 11122333 |
| nerve injury-associated kinase: a sterile 20-like protein kinase up-regulated in dorsal root ganglia in a rat model of neuropathic pain. | partial injury of the rat sciatic nerve elicits a variety of characteristic chemical, electrophysical and anatomical changes in primary sensory neurons and constitutes a physiologically relevant model of neuropathic pain. to elucidate molecular mechanisms that underlie the physiology of neuropathic pain, we have used messenger rna differential display to identify genes that exhibit increased ipsilateral expression in l4/5 dorsal root ganglia, following unilateral partial ligation of the rat scia ... | 2000 | 11113325 |
| the pdx-1 activation domain provides specific functions necessary for transcriptional stimulation in pancreatic beta-cells. | pdx-1 is a homeodomain transcription factor whose targeted disruption results in a failure of the pancreas to develop. mutations in the human pdx-1 gene are linked to an early onset form of non-insulin-dependent diabetes mellitus. pdx-1 binds to and transactivates the promoters of several physiologically relevant genes within the beta-cell, including insulin, glucose transporter 2, glucokinase, and islet amyloid polypeptide. this study focuses on the mechanisms by which pdx-1 activates insulin g ... | 2000 | 11117522 |
| expression in yeast and purification of functional recombinant human poly(adp-ribose)polymerase (parp). comparative pharmacological profile with that of the rat enzyme. | human poly(adp-ribose)polymerase (parp) was expressed in the yeast line jel1 under the control of a gal promoter. proteins were extracted and human recombinant parp purified to apparent homogeneity. the pharmacological profile of this human enzyme was characterised in terms of the effects of known inhibitors of parp belonging to various chemical families and this was compared with that of the rat enzyme purified from rat testes, using the same purification protocol. the rat and the human enzymes ... | 2000 | 11030086 |
| rat alpha-synuclein interacts with tat binding protein 1, a component of the 26s proteasomal complex. | the alpha-synuclein gene, which encodes a brain presynaptic nerve terminal protein of unknown function, is linked to familial early-onset parkinson's disease (pd). the finding that alpha-synuclein forms the major fibrillary component of lewy bodies in brains of pd patients suggests that the two point mutations in alpha-synuclein (ala(53)thr, ala(30)pro) may promote the aggregation of alpha-synuclein into filaments. to address the role of alpha-synuclein in neurodegenerative diseases, we performe ... | 2000 | 11032911 |
| the human preprotachykinin-a gene promoter has been highly conserved and can drive human-like marker gene expression in the adult mouse cns. | toward an understanding of the mechanisms controlling preprotachykinin-a (ppta) transcription, we introduced a 380-kb human yeast artificial chromosome containing the ppta gene tagged with the beta-galactosidase gene into transgenic mice. this resulted in a pattern of lacz expression in the central nervous system (cns) remarkably similar to that reported for ppta mrna in the rat. however, the human gene drove expression in areas of the mouse cns not associated with strong ppta expression in rode ... | 2000 | 11083923 |
| human clp36, a pdz-domain and lim-domain protein, binds to alpha-actinin-1 and associates with actin filaments and stress fibers in activated platelets and endothelial cells. | a 38-kd protein that associates with f-actin structures in activated platelets and endothelial cells was purified, cloned, and characterized. the protein contains an n-terminal pdz motif, a large intervening sequence, and a c-terminal lim domain and was identified as the human homolog of rat clp36. the study showed that clp36 associates with actin filaments and stress fibers that are formed during shape change and spreading of platelets and during migration and contraction of endothelial cells. ... | 2000 | 11110697 |
| dietary selenium and arsenic affect dna methylation in vitro in caco-2 cells and in vivo in rat liver and colon. | selenium is an essential trace element for human health, and it has received considerable attention for its possible role as an anticarcinogenic agent. the purpose of the present study was to determine whether changes in the amount and the chemical form of selenium would affect dna methylation and whether this effect would be modified by arsenic. caco-2 cells, a human colon cancer cell line, were exposed to 0, 1 or 2 micromol supplemental selenite/l and 0, 1 or 2 micromol supplemental arsenite/l ... | 2000 | 11110844 |
| a sequence-ready pac contig of a 550-kb region on rat chromosome 4 including the diabetes susceptibility gene lyp. | the lyp locus controls diabetes development in rats. the diabetogenic allele in diabetes-prone bb rats is responsible for t cell lymphopenia characterized by the absence of regulatory t cells. we present refined genetic and radiation hybrid maps of the lyp region on rat chromosome 4, a single 800-kb rat yeast artificial chromosome and a rat p1-derived artificial chromosome (pac) contig corresponding to approximately 550 kb, both encompassing the entire candidate region. the contig, consisting of ... | 2000 | 11056048 |
| lc3, a mammalian homologue of yeast apg8p, is localized in autophagosome membranes after processing. | little is known about the protein constituents of autophagosome membranes in mammalian cells. here we demonstrate that the rat microtubule-associated protein 1 light chain 3 (lc3), a homologue of apg8p essential for autophagy in yeast, is associated to the autophagosome membranes after processing. two forms of lc3, called lc3-i and -ii, were produced post-translationally in various cells. lc3-i is cytosolic, whereas lc3-ii is membrane bound. the autophagic vacuole fraction prepared from starved ... | 2000 | 11060023 |
| binding of coatomer by the pex11 c-terminus is not required for function. | microbodies are single membrane-bound organelles found in eukaryotes from trypanosomes to man. although they have diverse roles in metabolism, the mechanisms and molecules involved in membrane biogenesis and matrix protein import are conserved. similarly, the basic mechanisms and structures involved in vesicular transport are similar throughout eukaryotic evolution. the pex11 proteins are required for the division of microbodies in trypanosomes, yeast and mammals, and a role of coatomer in this ... | 2000 | 11068037 |
| reversible induction of rat hepatoma cell polarity with bile acids. | a dynamic model for inducing and isolating polarized cell colonies from differentiated rat hepatoma was established with chenodeoxycholic acid (cdca). cells were treated with 75 microm cdca in a 1% solvent mix (dmso/ethanol: 0.5%/0.5%) for 11 days and positive fao-ba1 and c2rev7-ba1 clones were isolated, respectively, from fao and c2rev7. cell polarization in these two clones was demonstrated by (i) the detection of (gamma)-glutamyl transpeptidase activity (gamma)-gt) and the presence of specifi ... | 2000 | 11069769 |
| role of prolactin in chloro-s-triazine rat mammary tumorigenesis. | chloro-s-triazine herbicides [cyanazine (cz), atrazine (az), simazine (sz)] increase mammary tumors in crl:cd br rats but not in f-344 rats or in mice. a nongenotoxic mechanism was investigated since the chloro-s-triazines are negative in short-term tests for genotoxicity. an in vivo battery was used to assess the chloro-s-triazines for estrogenic activity or for their ability to increase prolactin (prl) levels, both of which play important roles in enhancing mammary gland tumorigenesis in roden ... | 2000 | 11071396 |
| lectin binding patterns in rat nasal-associated lymphoid tissue (nalt) and the influence of various types of lectin on particle uptake in nalt. | we investigated the binding of four types of lectin to follicle-associated epithelium overlying the nasal-associated lymphoid tissue (nalt) of rats in order to identify m-cell specific surface markers and to determine the influence of lectin administration to nalt on the uptake of a particulate antigen. the nalt tissues were incubated with a panel of four types of lectin conjugated to horseradish peroxidase (hrp). ulex europaeus-1 (uea-1) and dolichos biflorus (dba) lectin stained the surface of ... | 2000 | 11073062 |
| genotoxic and mono-oxygenase system effects of the fungicide maneb. | the in vivo effects of a commercial preparation of maneb on mono-oxygenase activities of hepatic microsomes of basal and induced rats were examined. in vitro experiments with the d7 strain of yeast saccharomyces cerevisiae were also performed. in both basal and induced rats maneb caused a decrease in cytochrome p-450 content and aniline hydroxylase. immunoblotting analysis using anti-p-450 iie1 antibodies confirmed the data obtained for aniline hydroxylase activity. maneb was toxic in cells of s ... | 2000 | 11043497 |
| candida albicans and saccharomyces cerevisiae cell wall mannans produce fever in rats: role of nitric oxide and cytokines. | mannan components of c. albicans (5 mg/kg, i.p.) and s. cerevisiae (2.5 mg/kg, i.p.) cell walls produced pyrogenic responses which were completely inhibited by indomethacin (5 mg/kg, s.c.) pretreatment in rats. a non-selective nos inhibitor, l-name (10 mg/kg, s.c.), also inhibited the pyrogenic effectiveness of c. albicans mannan, whereas it was ineffective on the fever induced by s. cerevisiae mannan. a selective elevation in the serum tnf-alpha levels was observed at the initial phase of the f ... | 2000 | 11045605 |
| selenium regulates expression in rat liver of genes for proteins involved in iron metabolism. | suppression subtractive hybridization analysis in our laboratory recently revealed that transferrin mrna may be elevated in sedeficient rat liver. in this work, we compared expression in rat liver of genes for transferrin, transferrin receptor, ferritin light and heavy chains, and iron-regulatory proteins 1 and 2 in se adequacy and deficiency. weanling male sprague-dawley rats were fed torula yeast diets supplemented with 0 or 0.15 microg se/kg diet as sodium selenite for 15 wk. activity of cell ... | 2000 | 11049200 |
| regulation of ampa receptor glur1 subunit surface expression by a 4. 1n-linked actin cytoskeletal association. | the synaptic localization, clustering, and immobilization of neurotransmitter receptors and ion channels play important roles in synapse formation and synaptic transmission. although several proteins have been identified that interact with ampa receptors and that may regulate their synaptic targeting, little is known about the interaction of ampa receptors with the cytoskeleton. in studies examining the interaction of the ampa receptor glur1 subunit with neuronal proteins, we determined that glu ... | 2000 | 11050113 |
| creatine kinase, an atp-generating enzyme, is required for thrombin receptor signaling to the cytoskeleton. | thrombin orchestrates cellular events after injury to the vascular system and extravasation of blood into surrounding tissues. the pathophysiological response to thrombin is mediated by protease-activated receptor-1 (par-1), a seven-transmembrane g protein-coupled receptor expressed in the nervous system that is identical to the thrombin receptor in platelets, fibroblasts, and endothelial cells. once activated by thrombin, par-1 induces rapid and dramatic changes in cell morphology, notably the ... | 2000 | 11050237 |
| overexpression of da41 in v-ha-ras-3y1 cells causes growth suppression. | we have recently found that da41 exhibits marked homology with mouse plic-1, plic-2, frog xdrp1 and yeast dsk2. xdrp1 has been shown to be associated with cyclin a, and blocks cell division of frog embryo. in the present study, we examined the biological role(s) of da41 in mammalian cells by overexpressing it in v-ha-ras-transformed 3y1 cells (ras-3y1). transfectants which expressed a high level of da41 mrna exhibited a decrease in growth rate, a reduction in saturation density, and a suppressio ... | 2000 | 11050468 |
| the essential cofactor trrap recruits the histone acetyltransferase hgcn5 to c-myc. | the c-myc protein functions as a transcription factor to facilitate oncogenic transformation; however, the biochemical and genetic pathways leading to transformation remain undefined. we demonstrate here that the recently described c-myc cofactor trrap recruits histone acetylase activity, which is catalyzed by the human gcn5 protein. since c-myc function is inhibited by recruitment of histone deacetylase activity through mad family proteins, these opposing biochemical activities are likely to be ... | 2000 | 10611234 |
| identification of a human brain-specific isoform of mammalian ste20-like kinase 3 that is regulated by camp-dependent protein kinase. | a novel isoform of mammalian ste20-like kinase 3 (mst3) with a different 5' coding region from mst3, termed mst3b, was identified by searching through expressed sequence tag data base and obtained by rapid amplification of cdna 5'-ends. mst3b was assigned to the long arm of human chromosome 13, d13s159-d13s280, by use of the national center for biotechnology information sequence-tagged sites data base. reverse transcription-polymerase chain reaction and northern blot analysis with a probe derive ... | 2000 | 10644707 |
| molecular cloning and characterization of amida, a novel protein which interacts with a neuron-specific immediate early gene product arc, contains novel nuclear localization signals, and causes cell death in cultured cells. | amida was isolated by the yeast two-hybrid system as a novel protein which associated with arc, a non-transcriptional immediate early gene specific to the brain. amida was confirmed to be associated with arc in vitro and in vivo. amida shows no homology to known proteins. amida is ubiquitously expressed, although it is abundant in the brain. a transfection study revealed that amida was localized in the nucleus and after 72 h the transfected cells underwent apoptosis. furthermore, we found two nu ... | 2000 | 10644725 |
| nxp-1, a human protein related to rad21/scc1/mcd1, is a component of the nuclear matrix. | nuclear matrix is a complex intranuclear network supposed to be involved in the various nuclear functions. in order to identify the nuclear matrix proteins, we isolated a cdna clone from a human placenta cdna library. this clone was partially represented a known cdna clone ha1237. ha1237 encoded a 631-amino-acid peptide, which we designated nxp-1. nxp-1 was related to yeast rad21/scc1/mcd1, xenopus xrad21, and mouse pw29, and identical with hr21spa isolated from a human testis cdna library. we d ... | 2000 | 10623634 |
| an african swine fever virus erv1-alr homologue, 9gl, affects virion maturation and viral growth in macrophages and viral virulence in swine. | the african swine fever virus (asfv) genome contains a gene, 9gl, with similarity to yeast erv1 and alr genes. erv1 has been shown to function in oxidative phosphorylation and in cell growth, while alr has hepatotrophic activity. 9gl encodes a protein of 119 amino acids and was highly conserved at both nucleotide and amino acid levels among all asfv field isolates examined. monospecific rabbit polyclonal antibody produced to a glutathione s-transferase-9gl fusion protein specifically immunopreci ... | 2000 | 10627538 |
| neuronal inwardly rectifying k(+) channels differentially couple to pdz proteins of the psd-95/sap90 family. | several signaling proteins clustered at the postsynaptic density specialization in neurons harbor a conserved c-terminal pdz domain recognition sequence (x-s/t-x-v/i) that mediates binding to members of the psd-95/sap90 protein family. this motif is also present in the c termini of some inwardly rectifying k(+) (kir) channels. constitutively active kir2 channels as well as g protein-gated kir3 channels, which are fundamental for neuronal excitability, were analyzed as candidates for binding to p ... | 2000 | 10627592 |
| protein inhibitor of activated stat-1 (signal transducer and activator of transcription-1) is a nuclear receptor coregulator expressed in human testis. | an androgen receptor (ar) interacting protein was isolated from a hela cell cdna library by two-hybrid screening in yeast using the ar dna+ligand binding domains as bait. the protein has sequence identity with human protein inhibitor of activated signal transducer and activator of transcription (pias1) and human gu rna helicase ii binding protein (gbp). binding of pias1 to human ar dna+ligand binding domains was androgen dependent in the yeast liquid beta-galactosidase assay. activation of bindi ... | 2000 | 10628744 |
| the rna interacting domain but not the protein interacting domain is highly conserved in ribosomal protein p0. | protein p0 interacts with proteins p1alpha, p1beta, p2alpha, and p2beta, and forms the saccharomyces cerevisiae ribosomal stalk. the capacity of rpp0 genes from aspergillus fumigatus, dictyostelium discoideum, rattus norvegicus, homo sapiens, and leishmania infantum to complement the absence of the homologous gene has been tested. in s. cerevisiae w303dgp0, a strain containing standard amounts of the four p1/p2 protein types, all heterologous genes were functional except the one from l. infantum ... | 2000 | 10636918 |
| atpase-defective mammalian vps4 localizes to aberrant endosomes and impairs cholesterol trafficking. | the yeast vacuolar sorting protein vps4p is an atpase required for endosomal trafficking that couples membrane association to its atpase cycle. to investigate the function of mammalian vps4 in endosomal trafficking, we have transiently expressed wild-type or atpase-defective human vps4 (hvps4) in cultured cells. wild-type hvps4 was cytosolic, whereas a substantial fraction of hvps4 that was unable to either bind or hydrolyze atp was localized to membranes, including those of specifically induced ... | 2000 | 10637304 |
| intraperoxisomal localization of very-long-chain fatty acyl-coa synthetase: implication in x-adrenoleukodystrophy. | x-adrenoleukodystrophy (x-ald) is a demyelinating disorder characterized by the accumulation of saturated very-long-chain (vlc) fatty acids (>c(22:0)) due to the impaired activity of vlc acyl-coa synthetase (vlcas). the gene responsible for x-ald was found to code for a peroxisomal integral membrane protein (aldp) that belongs to the atp binding cassette superfamily of transporters. to understand the function of aldp and how aldp and vlcas interrelate in the peroxisomal beta-oxidation of vlc fat ... | 2000 | 10640429 |
| apobec-1 dependent cytidine to uridine editing of apolipoprotein b rna in yeast. | cytidine to uridine editing of apolipoprotein b (apob) mrna requires the cytidine deaminase apobec-1 as well as a tripartite sequence motif flanking a target cytidine in apob mrna and an undefined number of auxiliary proteins that mediate rna recognition and determine site-specific editing. yeast engineered to express apobec-1 and apob mrna supported editing under conditions of late log phase growth and stationary phase. the cis -acting sequence requirements and the intracellular distribution of ... | 2000 | 10606639 |
| [pharmacological studies of trilex on treatment of pharyngitis]. | trilex consisted of three species: ilex latifolia, ilex asprella and ilex rotunda. trilex had the antipyretic effects, decreased the rat body temperature about 0.8 degree c on yeast induced hyperthermia. trilex could obviously enhance the threshold of hot plate induced pain in mice. the maximum threshold increased 43.8%. trilex could also inhibit acetic acid induced inflammatory ooze in mice abdominal cavity. the inhibitory rate was 34.8%. these results showed that trilex had antipyretic, analge ... | 2000 | 12575042 |
| expression and catalytic activity of mouse leukotriene b4 omega-hydroxylase, cyp4f14. | we have isolated a cdna for a mouse leukotriene b4 omega-hydroxylase, cyp4f14. the cdna encoded a protein with 524 amino acids, whose sequence similarity is 95% that of rat cyp4f1. the microsomes from yeast cells transfected with cyp4f14 expression vector showed 0.1 nmol p450/mg protein and catalyzed omega-hydroxylations of leukotriene b4, 6-trans-leukotriene b4, lipoxin a4, prostaglandin a1, and several hydroxyeicosatetraeonic acids (hetes), with 8-hete being the most active substrate. in contr ... | 2000 | 11185557 |
| n-linked oligosaccharide processing enzyme glucosidase ii produces 1,5-anhydrofructose as a side product. | alpha-1,4-glucan lyase cleaves alpha-1,4-linkages of nonreducing termini of alpha-1,4-glucans to produce 1,5-anhydrofructose (1,5-anfru). the enzymes isolated from fungi and algae show high homology with glycoside hydrolase family 31. purification of alpha-1,4-glucan lyase from rat liver using deae cellulose chromatography resulted in separation of two enzymatic active fractions, one was bound to the column and the other was in the flow-through. partial amino acid sequence determined from the ly ... | 2000 | 11159920 |
| [screening the trans-action factors binding to the enhancer element of rat gst-p by yeast one-hybrid system]. | to investigate the diversity of the mechanisms of rat glutathione s-transferase p (gst-p) gene expression regulation and the relation between rat gst-p gene expression and carcinogenesis. to search the trans-action factors binding to the enhancer element of rat gst-p (glutathione s-transferase p enhancer, gpe i). | 2000 | 12903441 |
| active intracellular domain of notch enhances transcriptional activation of ccaat/enhancer binding protein beta on a rat pregnancy-specific glycoprotein gene. | pregnancy-specific glycoproteins (psgs) are primarily expressed in the placenta and become the major glycoproteins at term. to understand the regulation of psg expression, we characterized the promoter elements of a rodent psg gene, rncgm3, and delineated three nuclear protein binding sites: fpi, -ii, and -iii in the 5'-flanking region of the gene. the fpii-binding factor is shown to be c/ebpbeta, which positively regulates rncgm3 expression [chen, h., et al. (1995) dna cell biol. 14, 681-688]. ... | 2000 | 10677215 |
| pancreatic eukaryotic initiation factor-2alpha kinase (pek) homologues in humans, drosophila melanogaster and caenorhabditis elegans that mediate translational control in response to endoplasmic reticulum stress. | in response to different cellular stresses, a family of protein kinases regulates translation by phosphorylation of the alpha subunit of eukaryotic initiation factor-2 (eif-2alpha). recently, we identified a new family member, pancreatic eif-2alpha kinase (pek) from rat pancreas. pek, also referred to as rna-dependent protein kinase (pkr)-like endoplasmic reticulum (er) kinase (perk) is a transmembrane protein implicated in translational control in response to stresses that impair protein foldin ... | 2000 | 10677345 |
| an electric lobe suppressor for a yeast choline transport mutation belongs to a new family of transporter-like proteins. | choline is an important metabolite in all cells due to the major contribution of phosphatidylcholine to the production of membranes, but it takes on an added role in cholinergic neurons where it participates in the synthesis of the neurotransmitter acetylcholine. we have cloned a suppressor for a yeast choline transport mutation from a torpedo electric lobe yeast expression library by functional complementation. the full-length clone encodes a protein with 10 putative transmembrane domains, two ... | 2000 | 10677542 |
| conserved composition of mammalian box h/aca and box c/d small nucleolar ribonucleoprotein particles and their interaction with the common factor nopp140. | small nucleolar ribonucleoprotein particles (snornps) mainly catalyze the modification of rrna. the two major classes of snornps, box h/aca and box c/d, function in the pseudouridylation and 2'-o-methylation, respectively, of specific nucleotides. the emerging view based on studies in yeast is that each class of snornps is composed of a unique set of proteins. here we present a characterization of mammalian snornps. we show that the previously characterized nap57 is specific for box h/aca snornp ... | 2000 | 10679015 |