Publications

oxa1p, which is required for cytochrome c oxidase and atp synthase complex formation, is embedded in the mitochondrial inner membrane.	we have previously isolated the yeast nuclear gene oxa1 and showed that oxa1p is required for the formation of the cytochrome c oxidase and atp synthase complexes. we have expressed oxa1p in e. coli and shown that it is toxic and rapidly degraded. nevertheless, a truncated protein was successfully expressed and antibodies have been raised against this truncated protein. these antibodies recognise a protein in mitochondrially enriched fractions. in vitro mitochondrial import experiments demonstra ...	1997	9108137
progress towards using recombinant myxoma virus as a vector for fertility control in rabbits.	the history of myxoma virus, its use in australia as a mortality agent and the development of the virus as a vector for controlling fertility in wild rabbit populations in australia is reviewed. myxoma virus recombinants have been constructed to express model antigens. four potential insertion sites in the genome have been identified and two have been used to construct single and double recombinant viruses expressing escherichia coli enzymes beta-galactosidase and beta-glucuronidase. another rec ...	1997	9109196
a function for the qkraa amino acid motif: mediating binding of dnaj to dnak. implications for the association of rheumatoid arthritis with hla-dr4.	the amino acid motif qkraa, when expressed on hla-drb1, carries susceptibility to develop rheumatoid arthritis. this motif is the basis of strong b and t cell epitopes. furthermore, it is highly overrepresented in protein databases, suggesting that it carries a function of its own. to identify this function, we used qkraa peptide affinity columns to screen total protein extracts from escherichia coli. we found that dnak, the e. coli 70-kd heat shock protein, binds qkraa. of interest, dnak has a ...	1997	9109425
prevention of mucosal escherichia coli infection by fimh-adhesin-based systemic vaccination.	virtually all uropathogenic strains of escherichia coli, the primary cause of cystitis, assemble adhesive surface organelles called type 1 pili that contain the fimh adhesin. sera from animals vaccinated with candidate fimh vaccines inhibited uropathogenic e. coli from binding to human bladder cells in vitro. immunization with fimh reduced in vivo colonization of the bladder mucosa by more than 99 percent in a murine cystitis model, and immunoglobulin g to fimh was detected in urinary samples fr ...	1997	9110982
characterization of p0, a ribosomal phosphoprotein of plasmodium falciparum. antibody against amino-terminal domain inhibits parasite growth.	a cdna expression clone of the human malarial parasite plasmodium falciparum, lambdapf4, which was reactive only to the immune sera and not to the patient sera, has recently been found to be the p. falciparum homologue of the p0 ribosomal phosphoprotein gene. a northern analysis of the p0 gene revealed the presence of two transcripts, both present in all the different intraerythrocytic stages of the parasite life cycle. a 138-base pair amino-terminal domain of this gene was expressed as a fusion ...	1997	9115284
molecular, biological, and preliminary structural analysis of recombinant bryodin 1, a ribosome-inactivating protein from the plant bryonia dioica.	bryonia dioica (cucurbitaceae family) produces at least two type i ribosome-inactivating proteins, bryodin 1 (bd1) and bryodin 2 (bd2). a cdna sequence encoding bd1 was isolated from b. dioica leaf mrna using degenerative oligonucleotides and codes for a 22 amino acid signal peptide followed by a protein of 267 residues. expression of two recombinant bd1 (rbd1) forms in escherichia coli yielded proteins of 267 (to the natural stop codon) and 247 amino acids (to the putative cleavage site yieldin ...	1997	9115985
molecular cloning and expression of subunit 9 of the 26s proteasome.	seven peptides from subunit 9 (s9) of the human 26s proteasome were sequenced and this information was used to clone a hela cdna that encodes the 46 kda subunit. rabbit polyclonal antisera were made against a ubiquitin fusion protein containing 12 amino acids from s9 and against a full-length s9 expressed in e. coli. western blot analysis showed that the s9-specific antibodies bound the 26s proteasome and its regulatory complex separated on non-denaturing gels. in sds-page samples of the two com ...	1997	9119060
effects of low power laser-irradiation on differential blood count and body temperature in endotoxin-preimmunized rabbits.	low power laser irradiation has been shown to have various immune-modulatory effects under in vitro conditions but little is known about such effects in animal models. escherichia coli endotoxin-preimmunized rabbits were used to determine the influence of transcutaneously applied low power laser light on differential blood count and rectal temperature. after three initial immunizations animals were either boostered with 5 ng/kg of endotoxin or injected with pyrogen-free saline and subsequently u ...	1997	9129122
analysis of epitope structure of psp94 (prostate secretory protein of 94 amino acids): (i). immuno-dominant and immuno-recessive area.	psp94 is a potential biomarker for evaluating patients with prostate carcinoma. we have systematically studied the epitope structure of psp94 by using a polyclonal antibody against human psp94. results of peptide mapping and elisa tests of dose response to rabbit antiserum against human psp94 protein showed that only the n-terminal peptides (n30 and m23) are immunoreactive while all the synthetic peptides (c28, c10) located closer to the c-terminus are completely devoid of antigenic activity wit ...	1997	9136076
antibody reactivities to tumor-suppressor protein p53 and htlv-i tof, rex and tax in htlv-i-infected people with differing clinical status.	since the presence of anti-p53 antibody has been correlated with the mutation and accumulation of p53, the aim of this study was to detect anti-p53 antibody and understand its correlations with anti-tof, -rex, or -tax antibody reactivity in htlv-i infected people differing in their clinical status. a plasmid (pgex-tof) was constructed to express tof recombinant protein (rp) in escherichia coli. serum samples from 50 asymptomatic carriers (acs), 50 adult t-cell leukemia (atl) and 50 htlv-i-associ ...	1997	9139842
ono-5046, an elastase inhibitor, attenuates endotoxin-induced acute lung injury in rabbits.	endotoxin causes acute lung injury resembling acute respiratory distress syndrome. elastase, as well as reactive oxygen species released from activated neutrophils, are thought to play pivotal roles in the pathogenesis of this lung injury. this study investigated whether ono-5046, a specific elastase inhibitor, can attenuate acute lung injury induced by endotoxin in rabbits. thirty-two male anesthetized rabbits were randomly assigned to receive one of four treatments (n = 8 for each group): infu ...	1997	9141938
efficient autoproteolytic processing of the mhv-a59 3c-like proteinase from the flanking hydrophobic domains requires membranes.	the replicase gene of the coronavirus mhv-a59 encodes a serine-like proteinase similar to the 3c proteinases of picornaviruses. this proteinase domain is flanked on both sides by hydrophobic, potentially membrane-spanning, regions. cell-free expression of a plasmid encoding only the 3c-like proteinase (3clpro) resulted in the synthesis of a 29-kda protein that was specifically recognized by an antibody directed against the carboxy-terminal region of the proteinase. a protein of identical mobilit ...	1997	9143287
characterization of a large transferrin-binding protein from actinobacillus pleuropneumoniae serotype 7.	the binding of transferrin at the surface of actinobacillus pleuropneumoniae (a. pp.) is mediated by two proteins of approximately 60 and 100 kda. the 60 kda protein has been shown to be highly divergent among different serotypes and to induce a serotype-specific protective immune response. in this study we have characterized the 100 kda transferrin-binding protein of a. pp. serotype 7 and designated it as tfbb. the tfbb gene was found to be located immediately downstream of the tfba gene. it wa ...	1997	9151535
accessory subunit of mitochondrial dna polymerase from drosophila embryos. cloning, molecular analysis, and association in the native enzyme.	a full-length cdna of the accessory (beta) subunit of mitochondrial dna polymerase from drosophila embryos has been obtained, and its nucleotide sequence was determined. the cdna clone encodes a polypeptide with a deduced amino acid sequence of 361 residues and a predicted molecular mass of 41 kda. the gene encoding the beta subunit lies within 4 kilobase pairs of that for the catalytic subunit in the drosophila genome, on the left arm of chromosome 2. the two genes have similar structural featu ...	1997	9153213
delivery of primary autologous skeletal myoblasts into rabbit heart by coronary infusion: a potential approach to myocardial repair.	myocardial repair after injury is limited because the adult heart cannot regenerate. we propose using autologous skeletal muscle cells (myoblasts) as a source of reserve cells for repair of regions of damaged myocardium. this report examines two potential methods for the transfer of cells to the myocardium: selective coronary catheterization, and myoblast infusion or myoblast injection directly into the left ventricular wall. autologous, primary rabbit skeletal myoblasts were harvested, were tra ...	1997	9154641
a third secreted protein that is encoded by the enteropathogenic escherichia coli pathogenicity island is required for transduction of signals and for attaching and effacing activities in host cells.	enteropathogenic escherichia coli strains are able to signal host cells, cause dramatic cytoskeletal rearrangements, and adhere intimately to the cell surface in a process known as the attaching and effacing effect. a pathogenicity island of 35 kb known as the locus of enterocyte effacement (lee) is necessary and sufficient for this effect. the lee encodes an outer membrane adhesin called intimin, a type iii secretion apparatus, and the espa and espb secreted proteins. the dna sequence of the re ...	1997	9169753
prelytic and lytic conformations of erythrocyte-associated escherichia coli hemolysin.	flow cytometry was developed as a method to assess the conformation of erythrocyte-bound escherichia coli hemolysin polypeptide (hlya). topology of membrane-associated hemolysin (hlya(e)) was investigated by testing surface accessibility of hlya regions in lytic and nonlytic bound states, using a panel of 12 anti-hlya monoclonal antibodies (mabs). hemolysin associates nonlytically with erythrocytes at 0 to 2 degrees c. to test the hypothesis that the nonlytic hlya(e) conformation at 0 to 2 degre ...	1997	9169756
cdna cloning, expression, and rapid purification of a kunitz-type winged bean chymotrypsin inhibitor.	a 183-residue kunitz-type winged bean chymotrypsin inhibitor (wbci), inhibits its cognate protease at a molar ratio of 1:2, instead of the usual ratio of 1:1 common to other members of the family. from the cdna pool obtained by reverse transcription of the poly(a)+ rna of the developing winged bean seeds, the structural gene of wbci has been amplified by pcr using primers designed to delete the 24-residue signal peptide and introduce ecori and sali sites at the ends of the amplified dna. the lat ...	1997	9179296
structural basis for differential receptor binding of cholera and escherichia coli heat-labile toxins: influence of heterologous amino acid substitutions in the cholera b-subunit.	the closely related b-subunits of cholera toxin (ctb) and escherichia coli heat-labile enterotoxin (ltb) both bind strongly to gm1 ganglioside receptors but ltb can also bind to additional glycolipids and glycoproteins. a number of mutant ct b-subunits were generated by substituting ctb amino acids with those at the corresponding positions in ltb. these were used to investigate the influence of specific residues on receptor-binding specificity. a mutated ctb protein containing the first 25 resid ...	1997	9179843
release of replication-deficient retroviruses from a packaging cell line: interaction with glioma tumor spheroids in vitro.	the present study describes how various growth conditions affect gene expression and virus production from a retroviral packaging cell line (liz 9), grown as monolayers and as multicellular spheroids. in addition, to study the direct interaction between packaging cells and tumor tissue of glioma origin, liz 9 spheroids were confronted with tumor spheroids derived from a human glioma cell line, gamg. the results show a progressive gene transfer into the tumor tissue, with 9% transfection efficacy ...	1997	9180159
localization of the in vivo expression of p and f1 fimbriae in chickens experimentally inoculated with pathogenic escherichia coli.	escherichia coli causing septicemia in poultry often possess f1 (type 1) and/or p fimbriae which may be involved in bacterial colonization and infection. to investigate the expression of these fimbriae in vivo, two pathogenic e. coli strains with different fimbrial profiles, tk3 (fim+/pap+) and mt78 (fim+/pap-), were administered to 2-week-old chickens by either the intratracheal or caudal thoracic air sac inoculation route. antibodies specific for native f1 fimbriae were detected by elisa and i ...	1997	9188088
a putative monofunctional glycosyltransferase is expressed in ralstonia eutropha.	a gene, mgt, encoding a protein homologous to the n-terminal module of class a high-molecular-mass penicillin-binding proteins was identified in ralstonia eutropha. by using specific antibodies, the corresponding mgt protein was detected in association with the membrane, confirming that the n-terminal hydrophobic segment functioned as a membrane anchor. a derivative in which the hydrophobic sequence was deleted was overexpressed as a maltose-binding fusion protein in escherichia coli. cleavage o ...	1997	9190828
immunological cross reactivity of eaea (intimin) from e. coli that cause attaching and effacing lesions in humans and rabbits.		1997	9192001
binding of human enterotoxigenic escherichia coli expressing coli surface antigen 6 to rabbit intestinal enterocytes and glycoproteins.		1997	9192024
af/r2 adhesin and cytopathic effect as virulence traits of diarrhea-inducing escherichia coli o103 in european rabbit.		1997	9192028
hemolysin phenotypes and genotypes of eaea-positive and eaea-negative bovine verotoxigenic escherichia coli.	intimin or eaea protein has been implicated in the attaching/effacing lesion caused by entero-hemorrhagic escherichia coli (ehec) in the intestine but it is not produced by all ehec and is therefore not adequate as a marker for ehec. hemolysins are produced by a high percentage of verotoxigenic e. coli (vtec) and could be a marker for ehec, but their distribution and relation to virulence are not known. we used pcr amplification to determine the presence or absence of eaea sequences in 281 vtec ...	1997	9192033
localization of intravenously administered verocytotoxins (shiga-like toxins) 1 and 2 in rabbits immunized with homologous and heterologous toxoids and toxin subunits.	rabbits challenged intravenously with shiga toxin or with escherichia coli verocytotoxin 1 or 2 (vt1 or vt2) are known to develop diarrhea, paralysis, and death, which can be prevented by immunization with a toxoid. the pathological effects of vt1 in the central nervous system and the gastrointestinal tract of unimmunized rabbits correlate with the localization of 125i-vt1 in these tissues, whereas in immunized animals, localization of 125i-vt1 in target tissues is inhibited and labeled toxin is ...	1997	9199412
intranasal immunogenicity and adjuvanticity of site-directed mutant derivatives of cholera toxin.	genetically modified derivatives of cholera toxin (ct), harboring a single amino acid substitution in and around the nad binding cleft of the a subunit, were isolated following site-directed mutagenesis of the ctxa gene. two mutants of ct, designated cts106 (with a proline-to-serine change at position 106) and ctk63 (with a serine-to-lysine change at position 63), were found to have substantially reduced adp-ribosyltransferase activity and toxicity; ctk63 was completely nontoxic in all assays, w ...	1997	9199455
mutational analysis of substrate recognition by protein phosphatase 1.	the role of residues that are involved in substrate recognition by rabbit muscle protein phosphatase 1alpha (pp1) was investigated by site-directed mutagenesis and kinetic analyses using phosphorylase a, rii peptide, kemptide, and p-nitrophenyl phosphate as substrates. the atomic structure of pp1 has shown the active site to be at the confluence of three shallow grooves, a c-terminal groove, an acidic groove, and a hydrophobic groove. mutations of residues d208, d210, d212, e218, d220, e252, d25 ...	1997	9204865
microbial growth inside saline-filled breast implants.	in vitro and in vivo experiments were conducted to determine whether intraluminal saline in breast implants can support the growth of common wound-infecting microorganisms over a prolonged period of time. the bacteria tested were staphylococcus aureus, staphylococcus epidermidis, escherichia coli, corynebacterium jeikeium, enterobacter cloacae, klebsiella pneumoniae, and pseudomonas aeruginosa. three fungal species also were tested: aspergillus fumigatus, paecilomyces variotii, and candida albic ...	1997	9207676
identification of a fourth gene involved in dtdp-rhamnose synthesis in streptococcus mutans.	we had isolated three genes (rmla, rmlb, and rmlc) involved in dtdp-rhamnose synthesis in streptococcus mutans and found that three genes were insufficient for dtdp-rhamnose synthesis (y. tsukioka, y. yamashita, t. oho, y. nakano, and t. koga, j. bacteriol. 179:1126-1134, 1997). the rmld gene of s. mutans, encoding the enzyme which catalyzes the last step of dtdp-rhamnose synthesis, has been cloned and sequenced. the cell extract of escherichia coli expressing the rmld gene of s. mutans exhibite ...	1997	9209063
expression, purification, and enzymatic properties of recombinant human cytochrome p450c27 (cyp27).	a large number of microsomal p450s have been expressed in escherichia coli in quantities sufficient for structure/function analysis. however, only one mitochondrial p450 has been successfully overexpressed, that being cholesterol side chain cleavage cytochrome p450 (p450scc). we report here overexpression, purification, and characterization of a second mitochondrial p450, human sterol c-27 hydroxylase (p450c27). the conditions used for expression are very similar to those applied for p450scc, al ...	1997	9210654
cloning and analysis of a borrelia burgdorferi membrane-interactive protein exhibiting haemolytic activity.	we cloned the gene encoding a membrane-interactive protein of borrelia burgdorferi by means of its haemolytic activity in escherichia coli. the haemolytic activity was erythrocyte-species specific, with progressively decreasing activity for erythrocytes from horse, sheep, and rabbit, respectively. genetic analysis of the haemolytic determinant revealed two borrelia haemolysin genes, blya and blyb, that are part of a predicted four-gene operon which is present in multiple copies on the 30 kb circ ...	1997	9218769
dna-binding activity of the a-factor receptor protein and its recognition dna sequences.	the a-factor receptor protein (arpa) containing an alpha-helix-turn-alpha-helix dna-binding consensus sequence at its n-terminal portion plays a key role in the regulation of secondary metabolism and cell differentiation in streptomyces griseus. a binding site forming a palindrome 24bp in length was initially recovered from a pool of random-sequence oligonucleotides by rounds of a binding/immunoprecipitation/amplification procedure with histidine-tagged arpa and anti-arpa antibody. by means of f ...	1997	9220006
selective modification at the n-terminal region of human growth hormone that shows antagonistic activity.	a new analogue of recombinant human growth hormone (hgh), hgh des(1-6,14) was expressed in escherichia coli, refolded and purified to homogeneity. the mutation decreased the hormone's ability to bind lactogenic and somatogenic receptors through its site 1, and almost completely abolished its ability to bind these receptors through site 2, as evidenced by both binding and gel-filtration experiments. more specifically, the binding to prolactin receptors (prlrs) from various species or their solubl ...	1997	9220030
histidine-44 of the a subunit of escherichia coli enterotoxin is involved in its enzymatic and biological activities.	we examined the role in toxicity of histidine-44 of the a subunit of escherichia coli enterotoxin, which is located in the active site cavity close to glutamic acid-112. although amino acid substitution of histidine-44 usually renders a mutant toxin unstable to trypsin, one mutant, alanine-44 (his44ala) was found to be stable. his44ala did not show any agmatine:adp-ribosyltransferase activity in the presence or absence of recombinant adp-ribosylation factor. it showed no diarrheal or rabbit skin ...	1997	9231414
characterization of a recombinant fragment that contains a carbohydrate recognition domain of the filamentous hemagglutinin.	the filamentous hemagglutinin (fha) of bordetella pertussis plays an important role in establishing infection by attaching the bacteria to the ciliated respiratory epithelial cells. expression of dna encoding residues 1141 to 1279 of fha in escherichia coli yields a protein of 18,000 da that exhibits some of the carbohydrate recognition properties of fha (s. m. prasad, y. yin, e. rodzinski, e. i. tuomanen, and h. r. masure, infect. immun. 61:2780-2785, 1993). we have constructed an e. coli strai ...	1997	9234814
conformational changes in actin induced by its interaction with gelsolin.	actin cleaved by the protease from escherichia coli a2 strain between gly42 and val43 (ecp-actin) is no longer polymerizable when it contains ca2+ as a tightly bound cation, but polymerizes when mg2+ is bound. we have investigated the interactions of gelsolin with this actin with regard to conformational changes in the actin molecule induced by the binding of gelsolin. ecp-(ca)actin interacts with gelsolin in a manner similar to that in which it reacts with intact actin, and forms a stoichiometr ...	1997	9251809
localization of radiolabeled chemotactic peptide at focal sites of escherichia coli infection in rabbits: evidence for a receptor-specific mechanism.	the infection imaging properties of a high-affinity 99mtc-labeled chemotactic peptide receptor agonist (n-formyl-methionyl-leucyl-phenylalanine-lysine; n-for-mlfk) were compared with a low-affinity agonist (n-acetyl-mlfk; n-ac-mlfk), a moderate-affinity antagonist (n-isobutyloxycarbonyl-mlfk; n-iboc-mlfk) and non-specific inflammation imaging agents.	1997	9255175
effect of lipid modification on the physicochemical, structural, antigenic and immunoprotective properties of haemophilus influenzae outer membrane protein p6.	the outer membrane lipoprotein, p6 of haemophilus influenzae was studied to determine the importance of the native palmitoyl moiety on its physicochemical and immunological properties. a recombinant p6 (rp6) molecule devoid of lipidation signal sequence was expressed in escherichia coli and its properties were compared to those of the palmitylated protein purified from h. influenzae. the isoelectric point of rp6 was more acidic than that of the native protein and also exhibited less secondary st ...	1997	9261944
dissection of pathways implicated in integrin-mediated actin cytoskeleton assembly. involvement of protein kinase c, rho gtpase, and tyrosine phosphorylation.	a panel of antibodies to the alphaiibbeta3 integrin was used to promote adhesion of chinese hamster ovary cells transfected with the alphaiibbeta3 fibrinogen receptor. while some alphaiibbeta3 antibodies were not able to induce p125 focal adhesion kinase (p125fak) tyrosine phosphorylation, all the antibodies equally support cell adhesion but not spreading and assembly of actin stress fibers. absence of stress fibers was also obtained by plating on antibodies directed to the hamster beta1 integri ...	1997	9268301
the antimalarial drug, chloroquine, interacts with lactate dehydrogenase from plasmodium falciparum.	we have previously shown that a radioiodinated photoreactive analogue of chloroquine, [125i]n-(4-(4-diethylamino-1-methylbutylamino)quinolin-6-yl) -4-azido-2-hydroxybenzamide ([125i]asa-q), specifically labels two proteins in plasmodium falciparum with apparent molecular weights (mr) of 42 and 33 kda (foley m, deady lw, ng k, cowman af, tilley l. j biol chem 1994:269:6955-6961). we now report the identification of the 33 kda protein. the 33 kda protein was purified from plasmodium falciparum usi ...	1997	9274881
molecular cloning of the complementary dna for an additional member of the family of aortic aneurysm antigenic proteins.	we have purified and partially sequenced a protein from the adventitia of the human aorta (aortic aneurysm antigenic protein 40 kda; aaap-40) that has homologies to bovine aortic microfibril-associated glycoprotein (magp-36). it is immunoreactive with immunoglobulin g (iggs) purified from the serum and aortic wall of patients with abdominal aortic aneurysms. aaap-40 and magp-36 have fibrinogen-like and vitronectin-like motifs. screening an expression library constructed from human aortic adventi ...	1997	9279320
characterization of two virulence proteins secreted by rabbit enteropathogenic escherichia coli, espa and espb, whose maximal expression is sensitive to host body temperature.	enteropathogenic escherichia coli (epec) and rabbit epec (rdec-1) cause unique histopathological features on intestinal mucosa, including attaching/effacing (a/e) lesions. due to the human specificity of epec, rdec-1 has been used as an animal model to study epec pathogenesis. at least two of the previously identified epec-secreted proteins, espa and espb, are required for triggering host epithelial signal transduction pathways, intimate adherence, and a/e lesions. however, the functions of thes ...	1997	9284118
characterization of two f-actin-binding and oligomerization sites in the cell-contact protein vinculin.	vinculin, a structural protein of animal cells, is critically involved in the assembly of microfilament/plasma membrane junctions at cell contacts. to understand its role in organizing the distal portions of microfilaments into specific, morphologically distinct structures at these sites in more detail, we characterized its interaction with filamentous actin and with itself by means of in vitro assays. using recombinant proteins comprising different parts of the vinculin tail fused to the maltos ...	1997	9288940
a sensitive immunoassay to detect the alpha-chemokine gro in rabbit blood and lung fluids.	gro-alpha, gro-beta and gro-gamma are closely related peptides that stimulate growth of tumor cells and activate leukocytes in acute inflammatory reactions. in order to study the biology of gro peptides in the lungs of experimental animals, we have developed and characterized a sensitive and specific immunoassay for rabbit gro, and used this assay to measure gro in rabbit lung fluids and plasma. gro was cloned from a rabbit cdna library and expressed in escherichia coli. specific goat polyclonal ...	1997	9294594
purification and characterization of the protein kinase encoded by the ul13 gene of herpes simplex virus type 2.	the proteins encoded by the ul13 genes of herpes simplex virus types 1 (hsv-1) and 2 (hsv-2) have been predicted to be protein kinases. to identify the ul13 gene product, we have raised a rabbit polyclonal antiserum against a his.tag-hsv-1 ul13 fusion protein. the antibody specifically reacted with the 60-kda ul13 fusion protein expressed in escherichia coli and also recognized 56- to 57-kda late proteins in nuclear fractions of hsv-1- and hsv-2-infected cells. on the other hand, novel casein ki ...	1997	9300039
critical role of lipid composition in membrane permeabilization by rabbit neutrophil defensins.	we have examined the interactions of the six known rabbit neutrophil defensin antimicrobial peptides with large unilamellar vesicles (luv) made from various lipid mixtures based on the lipid composition of escherichia coli membranes. we find that the permeabilization of luv made from e. coli whole lipid extracts differs dramatically from that of single-component luv made from palmitoyl-oleoyl-phosphatidylglycerol (popg). specifically, defensins np-1, np-2, np-3a, np-3b, and a natural mixture of ...	1997	9305875
mammalian protein rap46: an interaction partner and modulator of 70 kda heat shock proteins.	a ubiquitously expressed nuclear receptor-associating protein of approximately 46 kda (rap46) was identified recently. interaction experiments with in vitro-translated proteins and proteins contained in cell extracts revealed that a great variety of cellular regulators associate with rap46. however, in direct interaction tests by the far-western technique, only 70 kda proteins showed up and were identified as members of the 70 kda heat shock protein (hsp70) family. interaction is specific since ...	1997	9312007
rabbit ubiquitin-activating enzyme e1: cdna cloning, sequence and expression.	a cdna clone encoding ubiquitin-activating enzyme e1 has been isolated from a rabbit heart cdna library and sequenced. the 3.485 kb cdna contains an open reading frame of 1058 amino acid residues which predicts a protein of approx. 118 kda. the deduced protein sequence exhibits a very high homology to other ubiquitin-activating enzymes identified in a variety of organisms. northern blot analysis reveals a single transcript of approx. 3.5 kb in all the rabbit tissues examined. the entire coding r ...	1997	9322736
expression of three functional domains of connexin 32 as thioredoxin fusion proteins in escherichia coli and generation of antibodies.	gap junctions, intercellular channels that allow cells to communicate directly, are constructed from connexin protein subunits. connexins traverse the membrane four times and have two extracellular loops and one intracellular loop; the amino and carboxyl tails are located at the intracellular aspect of the plasma membrane. the first extracellular domain (el1; residues 42-75), the intracellular domain (il; 94-130), the carboxyl-terminus (ct; 208-283), and the full-length rat connexin 32 (cx32) ge ...	1997	9325135
midgut-specific immune molecules are produced by the blood-sucking insect stomoxys calcitrans.	we have cloned and sequenced two defensins, smd1 and smd2, from anterior midgut tissue of the blood-sucking fly stomoxys calcitrans. the dna and n-terminal protein sequences suggest both are produced as prepropeptides. smd1 differs from the classic defensin pattern in having an unusual six-amino acid-long n-terminal sequence. both smd1 and smd2 have lower pi points and charge than insect defensins derived from fat body/hemocytes. northern analysis shows both of these defensin molecules are tissu ...	1997	9326639
colonic structural and ion transport abnormalities in suckling rabbits infected with escherichia coli k12.	escherichia coli k12 is a laboratory strain considered nonpathogenic. the purpose of this study was to examine the effect of e. coli k12 infection on colonic structure and function.	1997	9327369
[effects of human i.v. immunoglobulin on bacterial clearance and granulocyte function in endotoxinemia].	the therapeutic impact of intravenous immunoglobulins (ivig) in septic patients remains controversial. until now, the mechanisms of action have not been fully elucidated. since polymorphonuclear neutrophils (pmn) play a key role in host defence, this study focuses on the effects of ivig on bacterial clearance and pmn respiratory burst activity during endotoxinaemia. for this purpose, it was investigated whether ivig improves blood clearance and organ colonisation as well as pmn functions after e ...	1997	9340030
recognition of follicle stimulating hormone (alpha-subunit) by a recombinant receptor protein domain coded by an alternately spliced mrna and expressed in escherichia coli.	to assess the functional significance of putative proteins encoded by alternately spliced mrna of the sheep testicular fsh receptor, a short form cdna comprising of the first four exons (117 residues mature protein) was engineered for expression in escherichia coli. the expressed protein of molecular mass 15 kda was purified to homogeneity and verified by reaction with an antibody against a synthetic peptide sequence unique to the amino (n)-terminal region fsh receptor. the purified fsh receptor ...	1997	9343310
molecular cloning, sequencing, and expression in escherichia coli of mouse flavin-containing monooxygenase 3 (fmo3): comparison with the human isoform.	the sequence of mouse flavin-containing monooxygenase 3 (fmo3) was obtained from several clones isolated from a mouse liver cdna library. the nucleotide sequence of mouse fmo3 was 2020 bases in length containing 37 bases in the 5' flanking region, 1602 in the coding region, and 381 in the 3' flanking region. the derived protein sequence consisted of 534 amino acids including the putative flavin adenine dinucleotide and nadp+ pyrophosphate binding sites (characteristic of mammalian fmos) starting ...	1997	9344459
purification and characteristics of recombinant mouse metallothionein-i from escherichia coli.	the mouse metallothionein-i (mmt-i) cdna was amplified by polymerase chain reaction (pcr), inserted into vector pgex-4t-1, and expressed in escherichia coli as a carboxyl terminal extension of the 26-kda glutathione-s-transferase (gst). analyzed by sds-page, the amount of the expressed fusion protein gst-mt was over 50% of total cellular proteins. after the fusion protein had been digested with thrombin on a glutathione-sepharose 4b affinity chromatography column, recombinant mmt-i was purified ...	1997	9354383
a single amino acid substitution in ribonucleolytic toxin restrictocin abolishes its specific substrate recognition activity.	restrictocin is a small basic protein produced by the fungus aspergillus restrictus. it potently inhibits protein synthesis in eukaryotic cells by specifically cleaving a single phosphodiester bond in 28s rrna. a histidine residue at position 49 in restrictocin has been implicated in its active site. a mutant of restrictocin in which the histidine at position 49 was changed to an alanine was constructed by site-directed mutagenesis, and the protein was expressed in escherichia coli. the mutant a ...	1997	9354640
the product of the us10 gene of herpes simplex virus type 1 is a capsid/tegument-associated phosphoprotein which copurifies with the nuclear matrix.	we have identified the herpes simplex virus type 1 (hsv-1) us10 gene product using rabbit polyclonal antisera raised against a recombinant 6xhis-us10 fusion protein expressed in escherichia coli. the antiserum reacted specifically with 34 and 36 kda proteins in hsv-1 kos-infected cells as shown by western blotting and immunoprecipitation experiments. the 36 kda protein was immunoprecipitated with the us10 antiserum from 32p-labelled lysates of vero cells infected with hsv-1 kos, demonstrating th ...	1997	9367380
experimentally induced intrauterine infection causes fetal brain white matter lesions in rabbits.	periventricular leukomalacia, a common brain white matter lesion in preterm neonates, is a major risk factor for cerebral palsy. epidemiologic studies have demonstrated an association between infection and periventricular leukomalacia. the purpose of this study was to determine whether ascending intrauterine infection could cause brain white matter lesions in the fetal rabbit.	1997	9369822
glucose modulates hemodynamic, metabolic, and inflammatory responses to lipopolysaccharide in rabbits.	glucose is important for vascular and immunocompetent cell functions. we hypothesized that modifications in glucose metabolism (normal feeding, 24-h fasting, glucose loading) may influence the hemodynamic, metabolic, and inflammatory responses to lipopolysaccharide administration (lps; 600 micrograms/kg iv) in rabbits. aortic (abfv), hepatic artery (habfv), and portal vein blood flow velocities (pvbfv) (pulsed doppler), plasma tumor necrosis factor (tnf) and nitrites were measured. fasting deple ...	1997	9375321
expression of hbv pre s1 peptide in e. coli and product characterization.	hbv pre s1 sequence is supposed to play an important role in the infection of hbv. presence of pre s1/anti-pre s1 in serum has valuable clinical implications. in order to improve the study of pre s1, pre s1 sequence was overexpressed in e. coli as a fusion protein with mbp (maltose-binding protein), and anti-pre s1 antiserum was elicited in rabbits by pre s1-mbp purified by affinity chromatography. the recombinant plasmid constructed from pmal-cri expressed the 106aa pre s1 sequence at the c ter ...	1997	11243097
the production of recombinant dengue virus e protein using escherichia coli and pichia pastoris.	the dengue virus envelope protein was expressed as a gst fusion protein using e. coli and p. pastoris as expression hosts. in e. coli the recombinant e protein is expressed initially as a soluble 81 kda gst fusion protein. treatment of the fusion protein with thrombin released a 55 kda protein, which is the expected size for correctly processed, non-glycosylated recombinant e protein. the antiserum from animals immunised with this recombinant e protein was found to specifically recognise the den ...	1997	9504761
[the detection and study on enterotoxigenic escherichia coli in china].	in this paper, some strains of etec were first detected from the stools of the patients with cholerae-like diarrhea in china in 1974. with a detected rate 36.48% of etec detected rates weve 20.18% from patients with acute diarrhea, 19.74% from environment and sea foods. etec produced most of lt toxin in culture medium when the they were incubated at 37-42 degrees c for 4 days. when rabbit ileal loop test (rilt) was performed with the culture of etec or with germ-free culture supernatant, a large ...	1996	9387570
[expression of human brain myelin basic protein gene cdna in escherichia coli].	a 612bp full-length cdna sequence coding for 21.5 kd myelin basic protein (mbp) was amplified from human brain cdna library by polymerase chain reaction, cloned into vector pgem-3zf(+) with blunt-end. the 561bp cloned fragment was recovered from recombinant pgemp after bamhi digestion, and then inserted into the bamhi site downstream of the glutathione-s-transferase gene of the expression vector pgex-5t. the recombinant vector p5tbp was transformed into e. coli jm 109 and positive colonies were ...	1996	9389051
expression, purification and immunochemical characterization of recombinant bovine beta-lactoglobulin, a major cow milk allergen.	the immunological characteristics of a recombinant beta-lactoglobulin were studied using monoclonal antibodies, polyclonal antiserum and sera from allergic patients. recombinant beta-lactoglobulin (rblg) was expressed in escherichia coli strain dh5alpha and purified as described previously [cho et al. (1994) j. biol. chem. 269, 11 102-11 107]. the method has been modified by adding an immunoaffinity purification step. a quantity of 5-10mg of purified rblg per liter of medium culture can be produ ...	1996	9047377
unexpected carbohydrate cross-binding by escherichia coli heat-labile enterotoxin. recognition of human and rabbit target cell glycoconjugates in comparison with cholera toxin.	the bacterial protein enterotoxins, cholera toxin (ct) of vibrio cholerae and heat-labile toxin (lt) of escherichia coli, induce diarrhea by enhancing the secretory activity of the small intestine of man and rabbit (animal model). this physiological effect is mediated by toxin binding to a glycolipid receptor, the ganglioside gm1, gal beta 3galnac beta 4(neuac alpha 3)gal beta 4glc beta 1cer. however, lt, but not ct, was recently shown by us to bind also to paragloboside, gal beta 4glcnac beta 3 ...	1996	9007276
immunodetection of mitochondrial glycerophosphate dehydrogenase (mgdh) by a polyclonal antibody raised against a recombinant mgdh fragment product.	the mitochondrial enzyme glycerophosphate dehydrogenase (mgdh) plays an essential role in the b-cell glucose-sensing device and its activity in islet homogenates is impaired in several animal models of type 2 diabetes. we have now developed a polyclonal antibody, raised against a recombinant mgdh fragment product, that could be used for the immunodetection of mgdh. total rna was isolated from rat pancreatic islets and used in the synthesis of cdna. specific primers were designed that corresponde ...	1996	8986643
immunocytochemical localization of phenylalanine ammonia-lyase in tissues of populus kitakamiensis.	the polypeptide encoded by the partial fragment of cdna of phenylalanine ammonia-lyase (pal; ec 4.3.1.5), palcdna1 (osakabe et al., 1995, plant sci. 105: 217-226), isolated from populus kitakamiensis (p. sieboldii x p. grandidentata), was expressed in escherichia coli cells. the polypeptide was purified and an antiserum raised against it. the antiserum recognized a protein of 77 kda on nitrocellulose blots after sodium dodecyl sulfate-polyacrylamide gel electrophoresis of total protein and the p ...	1996	8987616
identification and mapping of a chromosomal gene cluster of borrelia burgdorferi containing genes expressed in vivo.	a clone containing a 6.4 kb borrelia burgdorferi chromosomal dna insert reacted only with sera from patients with lyme disease and not with any normal human or rabbit sera. restriction enzyme analysis indicated that this dna fragment was located on the b. burgdorferi chromosomal map between rpob and p22a; its direction of transcription was towards p22a. sequence analysis suggests that la006 encodes six proteins: three previously described immunodominant lipoproteins of the 39 kda bmp protein fam ...	1996	8978084
neutrophil activation and lung injury associated with chronic endotoxemia in rabbits.	chronic endotoxemia produces emphysematous lung destruction in several animal models. the present study was designed to examine changes in the polymorphonuclear leukocytes (pmn) and the lung parenchyma of rabbits that received either saline (control, n = 6) or escherichia coli endotoxin (lps, n = 6) 2-3 times weekly for 15 to 28 weeks. peripheral blood was collected just before and after each intravenous injection and lung tissue was processed at the end of the experiment. pmn myeloperoxidase wa ...	1996	8872088
the effect of combined rotavirus and escherichia coli infections in rabbits.	in rabbits, experimentally induced rotavirus infection results in soft feces only; thus it is unlikely that it is the sole cause of the severe, often fatal diarrhea of weanling rabbits with which it is associated. to determine whether rotavirus acts synergistically with another pathogen, new zealand white rabbits (10 to 38 weeks old) were inoculated with rotavirus (l:ala:84) and/or escherichia coli 015:h-(rdec-1) via orogastric tube. a single dose of high-titer (10(6) fluorescent focus-forming u ...	1996	8872987
isolation and characterization of hemolytic genes from actinobacillus actinomycetemcomitans.	periodontopathic actinobacillus actinomyceremcomitans produces hemolysin and other leukotoxins. in the present study, two distinct clones which lysed horse erythrocytes were isolated by screening genomic dna libraries of a. actinomycetemcomitans atcc 43718 on blood agar plates. dna hybridization analysis indicates that there were two distinct hemolytic genes present. sonicated extracts from both escherichia coli clones possessed hemolytic activities on horse, sheep and human erythrocytes, but no ...	1996	8837475
vector-derived expression of recombinant rat interleukin-6.	rat interleukin-6 (il-6) cdna, coding for an important inflammation- and immune-regulatory polypeptide cytokine, was cloned into the novel expression vector ph6ex3 which directs the synthesis of inserted genes as a fusion protein with histidine hexapeptide (hh). the resultant vector (pril6.992) was shown to produce significant amounts of recombinant rat il-6 fusion protein with hh at its n-terminus in various strains of escherichia coli. the expression of the hh-il-6 fusion protein was demonstra ...	1996	8860652
the calcium-binding protein calretinin-22k, an alternative splicing product of the calretinin gene is expressed in several colon adeno carcinoma cell lines.	an alternatively spliced mrna for the calcium-binding protein calretinin (cr) is present in the colon adenocarcinoma cell line widr. as a consequence of a frame shift, the resulting protein, calretinin-22k (cr-22k), consists of the first 178 amino acids of calretinin followed by a carboxy-terminal peptide of 14 amino acids that is not present in full-length calretinin. antibodies specific for this c-terminal region have been generated by 2 different methods. a peptide corresponding to the specif ...	1996	8864572
escherichia coli transcytosis in a caco-2 cell model: implications in neonatal necrotizing enterocolitis.	necrotizing enterocolitis (nec) is a serious gastrointestinal disorder of preterm infants. other than an association with prematurity and gastrointestinal feeding, no single factor or mechanism has been consistently linked to this disease. we have previously demonstrated that escherichia coli isolates obtained from the stool of infants with nec caused nec-like injury in a weanling rabbit ileal loop model; this injury, in turn, could be blocked by coinfection with selected gram(+) bacteria (enter ...	1996	8865278
pentoxifylline does not delay bacterially induced preterm delivery in rabbits.	our purpose was to determine whether pentoxifylline, a potent cytokine inhibitor, would delay bacterially induced preterm delivery in rabbits.	1996	8866298
mother to fry, successful transfer of immunity against infectious haematopoietic necrosis virus infection in rainbow trout.	we have tested whether immunity can be transferred from a mother fish to its fry. rainbow trout mother fish were inoculated against infectious haematopoietic necrosis virus (ihnv) by intraperitoneal injection of a fragment of the ihnv glycoprotein spanning amino acids 31 to 310. this protein fragment was obtained by isolating the specific cdna from japanese ihnv strain hv7601 and expressing it in escherichia coli. fry from immunized and control fish were exposed to ihnv at various intervals afte ...	1996	8887476
fine mapping of outer membrane protein p2 antigenic sites which vary during persistent infection by haemophilus influenzae.	antigenic drift of the major outer membrane protein (momp) p2 of nonencapsulated haemophilus influenzae as observed during persistent infections in patients with chronic bronchitis was mimicked in a rabbit model in which h. influenzae persisted in subcutaneous cages. the antigenic drift resulted from amino acid substitutions in potentially surface-exposed loops of momp p2. since in a rabbit model the appearance of antigenic variants was associated with the presence of strain-specific bactericida ...	1996	8890224
synthesis, processing, and oligomerization of bovine herpesvirus 1 ge and gi membrane proteins.	this study reports the identification and initial characterization of the precursors, modified forms, and oligomers of bovine herpesvirus 1 (bhv-1) gi and ge proteins with polyvalent rabbit serum specific for gi or ge. our experiments used the colorado strain of bhv-1 and mutant viruses with insertions of the escherichia coli lacz gene into the predicted ge and gi reading frames. we also translated the ge and gi open reading frames in vitro and expressed them in uninfected cells using eukaryotic ...	1996	8892910
polycistronic (tri- or bicistronic) phytoreoviral segments translatable in both plant and insect cells.	genomic segment s12 of rice dwarf virus and segment s9 of wound tumor virus, both members of the genus phytoreovirus, have small out-of-phase overlapping open reading frames (orfs). western blot (immunoblot) analysis revealed that rice dwarf virus s12 mrna specified translation products from the large orf and two overlapping small orfs both in rice plant hosts and in spodoptera frugiperda insect cells. these results provide the first example of a tricistronic mrna for a segmented double-stranded ...	1996	8892945
immune response to non-o157 vero toxin-producing escherichia coli in patients with hemolytic uremic syndrome.	the incidence and time course of the immune response to vero toxin-producing escherichia coli (vtec) other than o157 (o26, o55, o111, o128) were determined in 107 children with enteropathic hemolytic uremic syndrome (hus) by elisa and compared to antibody profiles of 125 healthy pediatric controls and 100 children with community-acquired uncomplicated diarrhea. six of 8 hus patients with non-o157 vtec isolates exhibited a serologic response to the homologous lipopolysaccharide antigen similar to ...	1996	8896505
hemoglobin exacerbates the ocular inflammatory response to endotoxin.	there is a clinical impression that bleeding into sites of inflammation exacerbates the inflammatory response. it has been hypothesized that hemoglobinic iron (fe) contributes to this response by catalyzing free radical reactions. in the present study, the effects of autologous hemoglobin on the inflammatory response to endotoxin was determined. in addition, the possible contributions of fe to this response was assessed by co-injection of either transferrin or desferrioxamine.	1996	8897058
chronic endotoxemia and endothelium-dependent vasodilation in coronary arteries.	endotoxin acutely decreases the production of nitric oxide, leading to abnormal regulation of coronary vascular tone: however, the effects of chronic endotoxemia on vasomotion are unknown. we therefore tested the hypothesis that chronic, low-level endotoxemia inhibits endothelium-dependent vasodilation. rabbits were continuously infused with a subclinical dose of escherichia coli endotoxin (.6 microgram/24 h, intraperitoneal) or saline for 5 wk. endotoxin at this concentration elicited no signif ...	1996	8902944
effects of n-acetylcysteine on bacterial clearance.	the aim of this study was to investigate whether the oxygen radical scavenger n-acetylcysteine (n-ac) impairs bacterial clearance, thus predisposing the host to increased risk of disease. blood clearance of escherichia coli and organ colonization were investigated in anaesthetized rabbits after pretreatment with n-ac (250 mg kg-1 body weight, n = 16) and in sham-operated animals (n = 12). to enable quantification of the clearance process, defined numbers of exogenous e. coli [1.3 x 108 colony-fo ...	1996	8911862
interconversion of the androstenedione hydroxylase specificities of cytochromes p450 2b4 and 2b5 upon simultaneous site-directed mutagenesis of four key substrate recognition residues.	based on recent studies of single reciprocal mutants of cytochrome p450 2b4 and the highly related p450 2b5 at positions 114, 294, 363, and 367 [g. d. szklarz, y. q. he, k. m. kedzie, j. r. halpert, and v. l. burnett (1996) arch. biochem. biophys. 327,308-318], a number of multiple mutants were constructed, expressed in escherichia coli, and assayed with androstenedione, progesterone, and benzyloxyresorufin. simultaneous substitutions of ile-114, ser-294, ile-363, and val-367 in cytochrome p450 ...	1996	8914846
rapid, efficient, large-scale purification of unfused, non-denatured e7 protein of cottontail rabbit papillomavirus.	cottontail rabbit papillomavirus (crpv) e7 protein is one of the :high risk' papillomavirus e7 oncoproteins that are partially insoluble in aqueous solution. an escherichia coli expression system was used for purification of crpv e7 protein in quantities sufficient for immunologic studies and structural analysis. a glutathione s-transferase (gst)-crpv e7 fusion protein was solubilized in the presence of non-ionic and ionic detergents, and isolated on an affinity column of glutathione sepharose b ...	1996	8919824
cholesterol 7alpha-hydroxylase activities from human and rat liver are modulated in vitro posttranslationally by phosphorylation/dephosphorylation.	purified cholesterol 7alpha-hydroxylases (c7alphah) from human and rat liver microsomes, and from transformed escherichia coli expression systems, were incubated with 0.3 mmol/l [gamma-32p] adenosine triphosphate (atp) in the presence and absence of bacterial alkaline phosphatase (ap) or rabbit muscle adenosine 3',5'-cyclic monophosphate (camp)-dependent protein kinase. the amounts of 32p incorporation after separation of human and rat c7alphah proteins by sodium dodecyl sulfate-polyacrylamide g ...	1996	8938182
purification of storage granule protein-23. a novel protein identified by phage display technology and interaction with type i plasminogen activator inhibitor.	type 1 plasminogen activator inhibitor (pai-1) is a key regulator of the fibrinolytic cascade that is stored in a rapidly releasable form within platelet alpha-granules. to identify proteins that may participate in the targeting or storage of this potent inhibitor, this report investigates the applicability of utilizing filamentous bacteriophages to display proteins expressed by cells containing a regulated secretory pathway and their enrichment based upon an interaction with pai-1. for this pur ...	1996	8939962
o serogroups, biotypes, and eae genes in escherichia coli strains isolated from diarrheic and healthy rabbits.	a total of 305 escherichia coli strains isolated from diarrheic and healthy rabbits in 10 industrial fattening farms from different areas of spain were serotyped, biotyped, and tested for the presence of the eae gene and toxin production. the characteristics found in strains isolated from healthy rabbits were generally different from those observed in e. coli strains associated with disease. thus, strains with the eae gene (74% versus 22%); strains belonging to serogroups o26, o49, o92, o103, an ...	1996	8940455
molecular characterization of a major serotype m49 group a streptococcal dnase gene (sdad).	group a streptococci (gas) express up to four types of secreted dnases. although gas infections are correlated with the production of anti-dnase b antibodies, the roles of dnases in the pathogenesis of gas infections remain unclear. from a lambda library of serotype m49 strain cs101 gas genome, a 2,147-bp fragment expressing dnase activity on an indicator agar was identified and sequenced. one 1,155-bp open reading frame (orf) was identified in this fragment. this orf was found to be 48% identic ...	1996	8945587
chimeric potyvirus-like particles as vaccine carriers.	presentation of subunit vaccines in a highly ordered aggregate form can result in enhanced immune responses. coat protein (cp) monomers of a potyvirus (johnsongrass mosaic virus) when produced in heterologous host expression systems (escherichia coli, yeast and insect cells) self-polymerized to produce potyvirus-like particles (pvlps). the n- and c-terminal regions of potyvirus cp are surface-exposed and are not required for assembly. hybrid cp monomers containing short peptides fused to their n ...	1996	8957674
heat-stable serotyping antigens expressed by strains of campylobacter jejuni are probably capsular and not long-chain lipopolysaccharide.	the role of lipopolysaccharide (lps) in the serotyping of campylobacter jejuni based on heat-stable antigens was examined using sds-page and a silver stain for carbohydrate. none of the 32 type strains of camp. jejuni expressed long-chain lps. rabbit antibodies, prepared to 10 selected strains of camp. jejuni, reacted with surface-exposed carbohydrate antigens, which were not lps. this study suggests that the heat-stable antigens of camp. jejuni, which form the basis for the established penner s ...	1996	8972090
normal levels of the vitamin d receptor and its message in psoriatic skin.	treatment with vitamin d3 analogs improves psoriasis. the vitamin d receptor (vdr) mediates most, if not all, the effects of vitamin d3. the purpose of this study was to determine the levels of the vdr mrna and vdr protein in normal and in involved and uninvolved psoriatic skin. although vdr mrna was not detected by northern analysis of human skin samples, it was readily detectable by use of the more sensitive ribonuclease protection assay. the vdr mrna levels were normal in acute guttate as wel ...	1996	9627688
use of prozz-obelin fusion protein in bioluminescent immunoassay.	obelin is a photoprotein that emits light by ca2+-binding. to develop a bioluminescent immunoassay based on the light emission property of obelin, we have expressed the apoobelin fusion protein with zz-domain of s. aureus protein a in e. coli by recombinant dna techniques. the prozz-obelin expressed was purified by one-step affinity chromatography on igg-agarose. the purified prozz-obelin has both the luminescent activity of obelin and the igg-binding ability of zz-domain. the specific activity ...	1996	8605012
identification of a set of proteins (c' and c) encoded by the bicistronic p gene of the indiana serotype of vesicular stomatitis virus and analysis of their effect on transcription by the viral rna polymerase.	previous work (c.f. spiropoulou and s.t. nichol, 1993, j. virol. 67, 3103-3110) has demonstrated the existence in cells infected with the new jersey serotype of vesicular stomatitis virus (vsv) of two small carboxy-coterminal proteins encoded by the p mrna. these proteins have been named c' and c. we are interested in studying the function of these proteins in the virus life cycle. toward this end, we have cloned the orf encoding the potential c' protein of the indiana serotype as a histidine-ta ...	1996	8610460
the protective role of enteral iga supplementation in neonatal gut-origin sepsis.		1996	8611004
lidocaine attenuates the hypotensive and inflammatory responses to endotoxemia in rabbits.	to assess the effects of lidocaine on the hemodynamic and inflammatory responses to escherichia coli endotoxemia in rabbits.	1996	8612417
anti-peptide immunoglobulins from rabbit and chicken eggs recognise recombinant human dihydroorotate dehydrogenase and a 44-kda protein from rat liver mitochondria.	mitochondrially bound dihydroorotate dehydrogenase catalyses the fourth sequential step in the de novo synthesis of uridine monophosphate. 312-bp and 983-bp regions of the human dihydroorotate dehydrogenase sequence (1496 bp) were amplified by the polymerase chain reaction, and subcloned into the expression vector pqe 32. the identity of the pcr products was verified by dideoxynucleotide sequencing. transformation of escherichia coli strain m15 resulted in expression of 13-kda and 36-kda protein ...	1996	8612635
interaction of verotoxin 2e with pig intestine.	in pigs with edema disease, verotoxin 2e (vt2e) is produced in the intestine and transported to tissues, but neither the mechanism by which toxin passes through the intestine nor its failure to induce an enterotoxic reaction is understood. binding of vt2e to pig intestine was examined by enzyme-linked immunosorbent assay involving microvillus membranes (mvm) and crude mucus; thin-layer chromatographic overlay immunoassay with total lipids extracted from mvm; and indirect immunofluorescence of to ...	1996	8613382