Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| priming and expression of immune responses in the gastric mucosa. | this review deals with the induction and expression of immune responses in the human stomach following mucosal immunisation. as prerequisites for developing a helicobacter pylori vaccine, the role of gastric inflammation, in particular inflammation induced by h. pylori infection, as well as lymphocyte homing within the common mucosal immune system, and the importance of immunisation route are discussed. | 2003 | 12814774 |
| animal models of helicobacter pylori infection and disease. | the acceptance of helicobacter pylori as a major human pathogen has necessitated the development of animal models to help elucidate the pathogenic mechanisms of this bacterium and aid in the development of improved strategies for the treatment of gastric disease. appropriate models, utilising a range of animal species, have been developed to examine factors such as the influence of host responses and bacterial factors in disease development and the success of new therapeutic regimens, including ... | 2003 | 12814775 |
| coexpression of helicobacter pylori's proteins caga and hspb induces cell proliferation in ags gastric epithelial cells, independently from the bacterial infection. | adenocarcinoma of the stomach is the second most common cause of cancer mortality in the world. the purpose of this study was to evaluate the potential role in carcinogenesis of two secreted helicobacter pylori's proteins, caga and hspb, both shown to increase the risk of gastric carcinoma in patients infected with h. pylori-positive strain. the effects of these two proteins on cell kinetics and the ability to selectively affect the expression of cell cycle-related proteins by transfection of a ... | 2003 | 14559823 |
| time-dependent amplified pharmacokinetic and pharmacodynamic responses of rabeprazole in cytochrome p450 2c19 poor metabolizers. | to determine the pharmacokinetic and pharmacodynamic rationale for the optimum regimen of rabeprazole in the treatment of helicobacter pylori infection in patients who are cytochrome p450 (cyp) 2c19 poor metabolizers or extensive metabolizers. | 2003 | 12820812 |
| induction of cyclooxygenase 2 by escherichia coli but not helicobacter pylori lipopolysaccharide in gastric epithelial cells in vitro. | cyclooxygenase 2 (cox-2) is an inducible enzyme that plays a key role in the synthesis of prostaglandins in response to inflammatory stimuli. it is expressed in the gastric mucosa as part of the response to infection with helicobacter pylori. the specific interaction between h. pylori and the gastric epithelium that results in cox-2 expression has not been identified. | 2003 | 14535998 |
| gastric transitional zones, areas where helicobacter treatment fails: results of a treatment trial using the sydney strain mouse model. | current combination therapies cure helicobacter pylori infection in 75 to 85% of cases. however, many treatment failures are not explained by antibiotic resistance. our goal was to explore treatment failures under in vivo conditions by using the h. pylori sydney strain (ss1) mouse model. mice infected with h. pylori (ss1) were treated with monotherapies or combination therapies used in human trials. bacterial levels and distribution of organisms within the stomach were assessed 24 h after treatm ... | 2003 | 12821476 |
| api2-malt1 fusion gene in colorectal lymphoma. | the api2-malt1 fusion gene was originally identified from a t(11;18)(q21;q21) translocation, a specific chromosomal abnormality that is found in mucosa-associated lymphoid tissue (malt) lymphoma. gastric malt lymphomas positive for the api2-malt1 fusion gene do not respond to helicobacter pylori-eradication therapy, but otherwise, the incidence and clinicopathological behavior of colorectal malt lymphoma with this genetic abnormality are unclear. we examined the api2-malt1 fusion by multiplex rt ... | 2003 | 14681324 |
| association between infections and signs and symptoms of 'atopic' hypersensitivity--results of a cross-sectional survey among first-year university students in germany and spain. | this study assessed the hypothesis that a decreased exposure to childhood infectious diseases is associated with signs and symptoms of 'atopic' hypersensitivity diseases. | 2003 | 12823114 |
| [chronic urticaria and infectious diseases]. | infectious diseases are often considered as a classic cause of chronic urticaria. nevertheless, laboratory investigations greatly vary from one centre to the other and the link between the infection and skin signs does not rely on hard data. the purpose of this work was a systematic analysis of the published cases of urticaria associated with infection. | 2003 | 12843809 |
| topology and membrane interaction of helicobacter pylori comb proteins involved in natural transformation competence. | the human gastric pathogen helicobacter pylori is naturally competent for genetic transformation. the h. pylori comb gene duster encodes the virb4-homologous atpase comb4 and the structural proteins comb7-comb10, which share significant sequence identity to the agrobacterium tumefaciens virb-encoded type iv secretion system. to study the topology of the comb7-10 proteins, we applied tnmax transposon mutagenesis by generating fusions of comb proteins with mature beta-lactamase (blam) or alkaline ... | 2003 | 12868652 |
| idiopathic interstitial pneumonia following stem cell transplantation. | idiopathic interstitial pneumonia (iip) can occur after stem cell transplantation, but the aetiology is unknown. based on the association between angiitis syndrome and helicobacter pylori infection, we identified possible risk factors common to these two conditions. among 83 patients who underwent stem cell transplantation, four developed iip. we elucidated various parameters and clinical features in four patients with iip and 79 patients without, after allogeneic stem cell transplantation. in a ... | 2003 | 12868990 |
| occurrence of a nontypable helicobacter pylori strain lacking lewis blood group o antigens and dd-heptoglycan: evidence for the role of the core alpha1,6-glucan chain in colonization. | the cell envelope of helicobacter pylori contains a lipopolysaccharide (lps) essential for the physical integrity and functioning of the bacterial cell membrane. the o-chain of this lps frequently expresses type 2 lewis x (lex) and lewis y (ley) blood group antigens that mimic human gastric mucosal cell-surface glycoconjugates. this article describes the isolation and structural analysis of the lps from a clinical isolate of h. pylori strain pj2 that lacks le antigens but is still capable of col ... | 2003 | 12907691 |
| recent advances in helicobacter pylori infection in children: from the petri dish to the playground. | helicobacter pylori infection is acquired in childhood, plays a causative role in chronic gastritis and peptic ulcer disease, and is associated with the development of gastric cancer. the present review focuses on recent advances in the scientific knowledge of h pylori infection in children, including clinical sequelae, diagnosis and treatment. in addition, recent insights regarding both bacterial and host factors that mediate human diseases associated with h pylori infection are discussed. | 2003 | 12915920 |
| human leukocyte antigen class ii genes and helicobacter pylori infection: does genotype overwhelm environmental exposure? | we investigated associations between human leukocyte antigen class ii genes, environmental exposures, and helicobacter pylori infection. | 2003 | 12921878 |
| il-1 rn 2/2 genotype and simultaneous carriage of genotypes il-1 rn 2/2 and il-1beta-511 t/t associated with oesophagitis in helicobacter pylori-negative patients. | interleukin-1 receptor antagonist genotype 2/2 is associated with a prolonged and enhanced inflammatory response. it is suspected of being a risk factor for atrophic gastritis and gastric cancer and for some autoimmune diseases. no specific genetic risk factors for oesophagitis have been identified so far and there are no reports of il-1 polymorphism in relation to oesophageal disease. | 2003 | 14750640 |
| review article: clinical significance of mucosal-protective agents: acid, inflammation, carcinogenesis and rebamipide. | while a great deal of clinical evidence has been found regarding anti-acids for the treatment of gastric disorders including peptic ulcers, not all disorders can be explained only by the hyperfunction of acid secretion. especially in the asian region, glandular atrophy is more prominent than in western countries, therefore low acid output is often observed in these patients. improvement of mucosal protection is rational therapy for these patients; this is the reason for use of these agents in as ... | 2003 | 12925154 |
| evaluation of helicobacter species in inflammatory bowel disease. | bacteria have been implicated in the pathogenesis of inflammatory bowel disease. helicobacter species have been shown to cause colitis in animal models and have been identified in human diarrhoeal illness and crohn's disease. | 2003 | 12950420 |
| treatment of helicobacter pylori infection using a novel antiadhesion compound (3'sialyllactose sodium salt). a double blind, placebo-controlled clinical study. | 3' sialyllactose sodium salt (3'sl) is an oligosaccharide that occurs naturally in human and bovine milk. it can inhibit the adhesion of h. pylori to human epithelial cells in vitro. the aim of this study was to test whether this oligosaccharide can suppress or cure h. pylori colonization in vivo and to determine its safety in humans. | 2003 | 12950597 |
| cytoskeletal rearrangements in gastric epithelial cells in response to helicobacter pylori infection. | helicobacter pylori causes host epithelial cell cytoskeletal rearrangements mediated by the translocation and tyrosine phosphorylation of an outer-membrane protein, caga, and by the vacuolating cytotoxin, vaca. however, the mechanisms by which h. pylori mediates cytoskeletal rearrangements in infected host cells need to be more clearly defined. the aim of this study was to determine the effects of h. pylori isolates from children on the architecture of host gastric epithelial cells. gastric epit ... | 2003 | 12972579 |
| acid-adaptive genes of helicobacter pylori. | helicobacter pylori is the only neutralophile that has been able to colonize the human stomach by using a variety of acid-adaptive mechanisms. one of the adaptive mechanisms is increased buffering due to expression of an acid-activated inner membrane urea channel, urei, and a neutral ph-optimum intrabacterial urease. to delineate other possible adaptive mechanisms, changes in gene expression in response to acid exposure were examined using genomic microarrays of h. pylori exposed to different le ... | 2003 | 14500513 |
| t(11;18)(q21;q21) in gastric malt lymphoma and diffuse large b-cell lymphoma of chinese patients. | t(11;18)(q21;q21) occurs at variable frequencies in mucosa-associated lymphoid tissue (malt) lymphomas of different sites. in gastric malt lymphoma, it occurs in 30% cases and the translocation positive tumours are frequently at advanced stages and do not respond to helicobacter pylori eradication. intriguingly, t(11;18)(q21;q21) has been observed only rarely in mucosal diffuse large b-cell lymphomas (dlbcl), despite that at least a proportion of these tumours are believed to be the result of tr ... | 2003 | 14502259 |
| cutting edge: cyclooxygenase-2 activation suppresses th1 polarization in response to helicobacter pylori. | helicobacter pylori infection causes a th1-driven mucosal immune response. cyclooxygenase (cox)-2 is up-regulated in lamina propria mononuclear cells in h. pylori gastritis. because cox-2 can modulate th1/th2 balance, we determined whether h. pylori activates cox-2 in human pbmcs, and the effect on cytokine and proliferative responses. there was significant up-regulation of cox-2 mrna and pge(2) release in response to h. pylori preparations. addition of cox-2 inhibitors or an anti-pge(2) ab resu ... | 2003 | 14530307 |
| plural transformation-processes from spiral to coccoid helicobacter pylori and its viability. | helicobacter pylori (h. pylori), present in a half of the world's population, is a very successful pathogen. the infection by this bacterium causes several gastric diseases in human. h. pylori is morphologically divided into two types; a spiral and a coccoid form. both types are observed in human stomach. although the former is converted into the latter in vitro, the process of coccoid formation remains obscure. furthermore, whether coccoid forms possess viability arouses much controversy among ... | 2003 | 12504609 |
| human beta-defensin-2 induction in helicobacter pylori-infected gastric mucosal tissues: antimicrobial effect of overexpression. | the objective of this study was to understand more of the innate immune response to helicobacter pylori by determining the expression of human beta-defensin-2 (hbd-2) in various gastric mucosal tissues and mkn45 gastric cancer cells with or without h. pylori. semi-quantitative taqman rt-pcr and immunohistochemistry were carried out. the antimicrobial effects of a transfected hbd-2 gene against h. pylori were also evaluated. the results showed that hbd-2 was expressed in inflamed gastric mucosal ... | 2003 | 12488564 |
| decreased epithelial cytokine responses in the duodenal mucosa of helicobacter pylori-infected duodenal ulcer patients. | helicobacter pylori colonizes the human stomach and areas of gastric metaplasia in the duodenum, but only a minority of those that are infected develop symptoms, e.g., peptic ulcers. although most ulcers occur in the duodenum, almost all studies of mucosal immune responses against the infection have been limited to responses in the stomach. in the present study we evaluated whether there are differences in the levels of proinflammatory cytokines as well as immunoregulatory cytokines in the duode ... | 2003 | 12522049 |
| is intestinal metaplasia of the stomach reversible? | intestinal metaplasia (im) of the stomach is a risk factor in developing intestinal-type gastric cancer and hence the question of reversibility is vital. there is emerging epidemiological evidence that with long term follow up, im may be reversible although a combination of antioxidant agents and eradication of h pylori may be necessary to achieve this. the pathogenesis of im is currently being elucidated and it is likely that a combination of bacterial, host, and environmental factors will be s ... | 2003 | 12477745 |
| multiple infections and subsequent cardiovascular events in the heart outcomes prevention evaluation (hope) study. | limited prospective epidemiological data are available on the relation between exposure to chlamydia pneumoniae, helicobacter pylori, cytomegalovirus (cmv), and hepatitis a virus (hav), individually or as a total pathogen score, and human cardiovascular (cv) disease. | 2003 | 12538424 |
| chemokine receptor 5 expression in gastric mucosa of helicobacter pylori-infected and noninfected children. | experimental data from human adults or animal models indicate that the helicobacter pylori-specific immune response is dominated by inflammatory t cells of the th1 type. to investigate whether a th1 immune response is established in early h. pylori infection, gastric biopsy samples from 70 children were subjected to immunohistochemical analysis. to this end, t cells, b cells, monocytes, neutrophils, and chemokine receptor 5 (ccr5)-expressing (ccr5(+)) cells, which are associated with th1 immune ... | 2003 | 12522035 |
| expression of lewis(b) blood group antigen in helicobacter pylori does not interfere with bacterial adhesion property. | the finding that some helicobacter pylori strains express lewis b (le(b)) blood group antigen casts a doubt on the role of le(b) of human gastric epithelium being a receptor for h. pylori. the aim of this study was to determine if expression of le(b) in h. pylori interferes with bacterial adhesion property. | 2003 | 12508365 |
| association of cyclooxygenase-2 expression with hp-caga infection in gastric cancer. | to observe the expression of cyclooxygenase-2 (cox-2) and to investigate the association between cox-2 expression and infection with cytotoxic-associated gene a (caga) positive strain helicobacter pylori (hp) in human gastric cancer, and subsequently to provide fresh ideas for the early prevention of gastric cancer. | 2003 | 12532440 |
| gastric epithelial reactive oxygen species prevent normoxic degradation of hypoxia-inducible factor-1alpha in gastric cancer cells. | the expression of hypoxia inducible factor (hif)-1alpha protein is tightly regulated by cellular oxygen status. namely, hif-1alpha protein is degraded rapidly in normoxic cells, whereas hypoxia stabilizes hif-1alpha to transactivate hypoxia-responsive genes. here we show that hif-1alpha protein is expressed aberrantly in gastric cancer cells under normoxia in a reactive oxygen species (ros)-dependent manner. the normoxic expression of hif-1alpha in concordance with its dna binding activity enhan ... | 2003 | 12538497 |
| impact of clarithromycin resistance and cyp2c19 genetic polymorphism on treatment efficacy of helicobacter pylori infection with lansoprazole- or rabeprazole-based triple therapy in japan. | helicobacter pylori treatment failure is thought to be due mainly to polymorphic cytochrome p450 2c19 (cpy2c19) genetic polymorphism, associated with proton pump inhibitor metabolism, and antimicrobial susceptibility. this report has ascertained which was more important, cpy2c19 polymorphism or antimicrobial susceptibility, when using 1-week lansoprazole-based or rabeprazole-based triple therapy in japan. | 2003 | 12544691 |
| helicobacter pylori induces apoptosis in barrett's-derived esophageal adenocarcinoma cells. | helicobacter pylori may protect against the development of dysplasia in barrett's epithelium of patients with gastroesophageal reflux disease. the aim of this study was to determine whether h. pylori preferentially induces apoptosis in barrett's-derived cancer cells compared to normal cells. a barrett's-derived adenocarcinoma cell line (oe33) was grown. h. pylori wild-type, isogenic vaca-, caga(-), and picb-/cage- mutant strains were grown on agar plates. intact or sonicated bacteria were used t ... | 2003 | 12559187 |
| [the prospects of vaccination against h. pylori infection]. | h. pylori causes numerous gastroduodenal diseases including malignancies. although eradication of h. pylori using antibiotics is clinically performed, it is sometimes unsuccessful because of resistant bacteria and patient non-compliance with treatment. therefore prophylactic and therapeutic vaccination against h. pylori infection is necessary for clinical use but it is still under study. h. pylori is widely believed to be transmitted in childhood. if long and chronic h. pylori infection causes g ... | 2003 | 12607329 |
| [tailor-made medicine in helicobacter pylori eradication therapy]. | pharmacokinetic profiles of omeprazole and lansoprazole were well correlated with the cyp2c19 genotype. the heterozygous extensive metabolizer was slightly different from the homozygote, but there was no statistically significant difference. the cyp2c19 genotype dependence found for lansoprazole was not obvious compared with omeprazole. as for rabeprazole, the pharmacokinetic profile was independent of the cyp2c19 genotype. cyp2c19 genotyping can provide a new strategy to choose an optimal regim ... | 2003 | 12607330 |
| the dendritic cell-specific c-type lectin dc-sign is a receptor for schistosoma mansoni egg antigens and recognizes the glycan antigen lewis x. | schistosoma mansoni soluble egg antigens (seas) are crucially involved in modulating the host immune response to infection by s. mansoni. we report that human dendritic cells bind seas through the c-type lectin dendritic cell-specific icam-3-grabbing nonintegrin (dc-sign). monoclonal antibodies against the carbohydrate antigens lewisx (lex) and galnacbeta1-4(fucalpha1-3)glcnac (ldnf) inhibit binding of dc-sign to seas, suggesting that these glycan antigens may be critically involved in binding. ... | 2003 | 12626400 |
| endotoxic and immunobiological activities of a chemically synthesized lipid a of helicobacter pylori strain 206-1. | a synthetic lipid a of helicobacter pylori strain 206-1 (compound hp206-1), which is similar to its natural lipid a, exhibited no or very low endotoxic activities as compared to escherichia coli-type synthetic lipid a (compound 506). furthermore, compound hp206-1 as well as its natural lipid a demonstrated no or very low mitogenic responses in murine spleen cell. on the other hand, compound hp206-1 showed a weaker but significant production of interleukin-8 in a gastric cancer cell line, mkn-1, ... | 2003 | 12727359 |
| disruption of the epithelial apical-junctional complex by helicobacter pylori caga. | helicobacter pylori translocates the protein caga into gastric epithelial cells and has been linked to peptic ulcer disease and gastric carcinoma. we show that injected caga associates with the epithelial tight-junction scaffolding protein zo-1 and the transmembrane protein junctional adhesion molecule, causing an ectopic assembly of tight-junction components at sites of bacterial attachment, and altering the composition and function of the apical-junctional complex. long-term caga delivery to p ... | 2003 | 12775840 |
| increased zinc finger protein zfoc1 transcripts in gastric cancer compared with normal gastric tissue. | screening of cdna arrays of the image library identified human zfoc1 as a differentially expressed cdna that was upregulated in kato iii gastric cancer cells following stimulation with the gastric pathogen helicobacter pylori. | 2003 | 12782764 |
| long-term treatment with sterigmatocystin, a fungus toxin, enhances the development of intestinal metaplasia of gastric mucosa in helicobacter pylori-infected mongolian gerbils. | helicobacter pylori is a human gastric carcinogen. sterigmatocystin (st), a fungus toxin, is a risk factor of gastric cancer. cytotoxin-vacuolation toxin a (vaca) present in supernatants of h. pylori suspensions can cause gastritis and ulcer. the aim of this study was to examine the effects of h. pylori, st and vaca in mongolian gerbils. | 2003 | 12739707 |
| t helper type 1 lymphocytes drive inflammation in human atherosclerotic lesions. | atherosclerotic lesions are infiltrated by macrophages and t lymphocytes, potentially reactive to pathogens. we studied in vivo activated t lymphocytes that infiltrate atherosclerotic plaques of helicobacter pylori-infected patients with or without anti-chlamydia pneumoniae antibodies. in all atherosclerotic lesions, t helper type 1 (th1) cells were predominant. c. pneumoniae-specific t cells were detected only in the plaques of anti-c. pneumoniae seropositive patients, whereas h. pylori-specifi ... | 2003 | 12740434 |
| strategies for the enrichment and identification of basic proteins in proteome projects. | two-dimensional gel electrophoresis (2-de) is currently the method of choice for separating complex mixtures of proteins for visual comparison in proteome analysis. this technology, however, is biased against certain classes of proteins including low abundance and hydrophobic proteins. proteins with extremely alkaline isoelectric points (pi) are often very poorly represented using 2-de technology, even when complex mixtures are separated using commercially available ph 6-11 or ph 7-10 immobilize ... | 2003 | 12748937 |
| review article: nsaids, gastroprotection and cyclo-oxygenase-ii-selective inhibitors. | in patients at high risk of nsaid-associated serious upper gastrointestinal complications, gastroprotection with misoprostol or a proton pump inhibitor should be considered. only misoprostol, 800 micro g/day, has been shown to reduce serious upper gastrointestinal complications in a large clinical outcome trial. the benefit of helicobacter pylori eradication in reducing nsaid-associated gastrointestinal toxicity is controversial, and routine testing for and eradication of h. pylori in nsaid user ... | 2003 | 12562444 |
| cell adhesion-related gene expression by helicobacter pylori in gastric epithelial ags cells. | helicobacter pylori (h. pylori) infection leads to gastroduodenal inflammation, peptic ulceration and gastric carcinoma. h. pylori may induce disease-specific gene expression in gastric epithelial cells. cdna microarray for 352 cancer-related genes was used to identify the genes altered by h. pylori (caga positive) in gastric epithelial ags cells. expressions of the genes identified on the microarray and other genes closely associated with these genes were determined by reverse transcriptase-pol ... | 2003 | 12757765 |
| helicobacter pylori infection affects the expression of pcna, p53, c-erbb-2 and bcl-2 in the human gastric mucosa. | helicobacter pylori infection has been related to gastric carcinogenesis. this association is based on epidemiological data, pathological changes observed in the gastric mucosa, and chemical products from bacteria that may induce damage of dna. in the present study we examined gastric endoscopic biopsies from patients with chronic gastritis, with and without h. pylori infection, and surgical biopsies from gastric cancer patients to evaluate whether this bacteria may induce changes in the express ... | 2003 | 12760717 |
| the rapid detection of low molecular mass proteins differentially expressed under biological stress for four helicobacter spp. using proteinchip technology. | helicobacter pylori is one of the most prevalent human pathogens in the world and is the aetiological agent of gastritis, peptic ulcer disease and gastric malignancies. in addition h. pylori and other novel members of the genus are capable of successfully colonising the bile-rich niche of the upper intestine and are associated with a diverse range of intestinal pathologies. surface-enhanced laser desorption/ionisation-time of flight mass spectrometry was used to analyse surface extracts from h. ... | 2003 | 12627380 |
| the impact of parietal cells on helicobacter pylori tropism and host pathology: an analysis using gnotobiotic normal and transgenic mice. | helicobacter pylori infection of the human stomach is common and typically benign, although a subset of hosts develops severe pathology. infection occurs in an organ with distinct microenvironments characterized by pronounced differences in the composition of acid-producing parietal cells. in this study, we examine determinants of bacterial tropism to various gastric niches by using germ-free normal and transgenic mice with an engineered parietal cell ablation. mice were colonized for 8 weeks wi ... | 2003 | 12629225 |
| remission of severe anemia persisting for over 20 years after eradication of helicobacter pylori in cases of ménètrier's disease and atrophic gastritis: helicobacter pylori as a pathogenic factor in iron-deficiency anemia. | a man with a 20-year history of recurrent iron-deficiency anemia complicated by helicobacter pylori-positive ménètrier's disease was observed over a 10-year clinical course, during which time he was successfully treated for the anemia and a gastric helicobacter pylori (h. pylori) infection through eradication. considering the satisfactory therapeutic results in this case, we performed eradication therapy on another h. pylori-positive atrophic gastritis case with a 24-year history of iron-deficie ... | 2003 | 14606710 |
| helicobacter pylori in indian hiv infected patients. | helicobacter pylori is a causative organism for chronic gastritis and associated with peptic ulcer disease. infection may be asymptomatic as well. human immuno-deficiency virus infection predisposes to a multitude of opportunistic infections, many of them resulting in gastrointestinal symptoms. we studied the prevalence of h pylori co-infection with hiv and its correlation with gastrointestinal symptoms in hiv infected patients. seventy-three consecutive hiv infected patients presenting to the m ... | 2002 | 12632975 |
| transcriptional regulation by thiol compounds in helicobacter pylori-induced interleukin-8 production in human gastric epithelial cells. | reactive oxygen species (ros) have been counted among the potential toxic factors involving helicobacter pylori (h. pylori)-induced gastric injury. transcription nuclear factor-kappab (nf-kappab) is activated by ros and regulates inflammatory gene expression. thiol compounds, such as glutathione and n-acetylcysteine, scavenge hydrogen peroxide and are reported to prevent oxidative damage in various cells. the present study aims to investigate whether thiol compounds could affect h. pylori-induce ... | 2002 | 12485925 |
| [relationship between helicobacter pylori infection and expressions of tumor suppressor genes in gastric carcinoma and related lesions]. | helicobacter pylori (hp) are believed to be a carcinogen of gastric carcinoma. however, its mechanism was yet not clearly understood. p53, p21waf1, and p16 are main negative regulator genes of cell cycle. this study was designed to investigate the relationship between these 3 genes and hp infection. | 2002 | 12508544 |
| [expression of pi glutathione s-transferase in intestinal metaplasia and its relationship with helicobacter pylori infection]. | to study the dynamic change of glutathione s- transferase pi (gst-pi) in normal gastric mucosa, gastric mucosa with intestinal metaplasia (im) and gastric cancer and to investigate the relationship between human carcinogen detoxification system and the virulence of helicobacter pylori(h.pylori) in the stage of im. | 2002 | 12194793 |
| helicobacter pylori organisms induce expression of activation and apoptotic surface markers on human lymphocytes and ags cells: a cytofluorimetric evaluation. | human peripheral blood mononuclear cells (pbmcs) were treated with helicobacter pylori (hp) organisms alone or with hp-stimulated ags cells (a gastric adenocarcinoma cell line). hp organisms were able per se to increase the percentage of cd8 +/- cd95 +/- cells, while number of cd25+ cells and hla-dr molecule expression increased following pretreatment with hp-stimulated ags cells. a comparison was made with a test system in which pbmcs were stimulated with escherichia coli (ec) organisms and col ... | 2002 | 12510791 |
| topographic association of gastric epithelial expression of ki-67, bax, and bcl-2 with antralization in the gastric incisura, body, and fundus. | helicobacter pylori (h. pylon) infection seems to induce antralization (ie., gastric mucosal transformation from transitional or body type to antral type), which is strongly associated with gastric atrophy and intestinal metaplasia. the aim of this study was to determine the topographic associations of ki-67 (a protein expressed in proliferative cells), bax (a pro-apoptotic protein), and bcl-2 (an antiapoptotic protein) expression with antralization. | 2002 | 12492185 |
| celecoxib versus diclofenac and omeprazole in reducing the risk of recurrent ulcer bleeding in patients with arthritis. | current guidelines recommend that patients at risk for ulcer disease who require treatment for arthritis receive nonsteroidal antiinflammatory drugs (nsaids) that are selective for cyclooxygenase-2 or the combination of a nonselective nsaid with a proton-pump inhibitor. we assessed whether celecoxib would be similar to diclofenac plus omeprazole in reducing the risk of recurrent ulcer bleeding in patients at high risk for bleeding. | 2002 | 12501222 |
| deregulation of shp-2 tyrosine phosphatase by the helicobacter pylori virulence factor caga. | helicobacter pylori (h. pylori) is estimated to infect about half of the world population. it causes gastric diseases ranging from gastritis to cancer and has been classified as a class i carcinogen by who. however, little is known about the molecular mechanisms by which h. pylori induces pathogenesis. caga is the product of the caga gene carried among virulent h. pylori strains and is associated with severe clinical outcomes, most notably gastric carcinoma. caga is injected from the attached h. ... | 2002 | 12528933 |
| synchronous primary adenocarcinoma, mucosa-associated lymphoid tissue lymphoma and a stromal tumor in a helicobacter pylori-infected stomach. | we report the case of a 78-year-old man with helicobacter pylori infection and three primary neoplasms of the stomach: adenocarcinoma, mucosa-associated lymphoid tissue (malt) lymphoma and a gastrointestinal stromal cell tumor. helicobacter pylori infection is associated with gastric carcinoma and malt lymphoma, although their simultaneous occurrence is rare. gastrointestinal stromal cell tumors have not been associated with infection to date. this appears to be the first report of the synchrono ... | 2002 | 12167128 |
| pathogenesis of helicobacter pylori infection. | five years after publication of the complete genome sequence of helicobacter pylori, research interest is shifting from the descriptive association of virulence factors with clinical outcome in infected patients to the molecular mechanisms of virulence factor action. this is particularly noticeable for vaca and caga, for both of which detailed understanding of the interaction with host signalling pathways has accumulated over the last year. the role of h. pylori lewis antigens for clinical outco ... | 2002 | 12197905 |
| helicobacter pylori virulence genes and host il-1rn and il-1beta genes interplay in favouring the development of peptic ulcer and intestinal metaplasia. | helicobacter pylori infection outcome might depend on genotypic polymorphisms of both the bacterium and the host. we ascertained: (1) the functionality of h. pylori oipa gene; (2) the polymorphism of the hostinterleukin (il-1beta) gene (-31 c/t) and of the il-1rn gene (intron 2 vntr); (3) the association between the above genes and the histological and pathological outcome of h. pylori infection. one hundred and sixty-five h. pylori positive and 137 h. pylori negative subjects (23 gastric adenoc ... | 2002 | 12161099 |
| expression of cyclooxygenase-2 in primary and remnant gastric carcinoma: comparing it with p53 accumulation, helicobacter pylori infection, and vascular endothelial growth factor expression. | cyclooxygenase-2 (cox-2) expression may contribute to the synthesis of prostanoids, which have been related to carcinogenesis and tumor progression. it is well known that the gastric remnant is at greater risk of the development of gastric cancer than is the whole stomach; incidence rates for gastric cardia adenocarcinoma are rising in the united states and europe. our objective was to determine the involvement of cox-2 in primary and remnant gastric cancer tissues as well as in adjacent noncanc ... | 2002 | 12173384 |
| inhibitory effect of lactobacillus acidophilus on helicobacter pylori in peptic ulcer patients: in vitro study. | lactobacillus acidophilus (l. acidophilus) have been introduced into many fermented dairy products. the presence of l. acidophilus appears to decrease helicobacter pylori (h. pylori) density in the human stomach and could enhance antibiotic therapy for h. pylori eradication. this study was designed to determine the optimal density of l. acidophilus that has a maximum inhibitory effect on h. pylori in peptic ulcer patients. to determine whether l. acidophilus has an inhibitory effect on h. pylori ... | 2002 | 12188456 |
| effect of weissella confusa strain pl9001 on the adherence and growth of helicobacter pylori. | viable and nonviable weissella confusa strain pl9001 inhibited the binding of helicobacter pylori to human gastric-cell line mkn-45 cells by more than 90%. spent culture supernatant of pl9001 rapidly decreased the viability of h. pylori, rupturing cell walls. the results suggest that pl9001 is a probiotic that can reduce the infectivity and persistence of h. pylori. | 2002 | 12200324 |
| shp-2 tyrosine phosphatase as an intracellular target of helicobacter pylori caga protein. | helicobacter pylori caga protein is associated with severe gastritis and gastric carcinoma. caga is injected from the attached helicobacter pylori into host cells and undergoes tyrosine phosphorylation. wild-type but not phosphorylation-resistant caga induced a growth factor-like response in gastric epithelial cells. furthermore, caga formed a physical complex with the src homology 2 domain (sh2)-containing tyrosine phosphatase shp-2 in a phosphorylation-dependent manner and stimulated the phosp ... | 2002 | 11743164 |
| helicobacter pylori may survive ampicillin treatment in the remnant stomach. | helicobacter pylori (h. pylori) is a gram-negative curved rod-like or spiral bacterium that chronically infects the human gastric mucosa, and is a major risk factor for gastritis, gastric and duodenal ulcer and adenocarcinoma of the stomach. after partial gastrectomy, some patients may have persistent h. pylori infection for five years or more. in this study, we detected three bacteria, i.e., klebsiella pneumoniae, enterobacter aerogenes, and escherichia coli, in the gastric juice of patients wi ... | 2002 | 12139018 |
| genome-scale metabolic model of helicobacter pylori 26695. | a genome-scale metabolic model of helicobacter pylori 26695 was constructed from genome sequence annotation, biochemical, and physiological data. this represents an in silico model largely derived from genomic information for an organism for which there is substantially less biochemical information available relative to previously modeled organisms such as escherichia coli. the reconstructed metabolic network contains 388 enzymatic and transport reactions and accounts for 291 open reading frames ... | 2002 | 12142428 |
| new strategies for the prevention and treatment of helicobacter pylori infection. | helicobacter pylori infects the stomach of > 50% of the human population worldwide, with higher prevalence in the developing countries. a strict correlation between h. pylori infection and gastroduodenal diseases has been demonstrated, including gastritis, peptic ulcer and gastric cancer. current therapies against h. pylori consist of an antisecretory plus antibiotics. these therapies are effective in 80 - 90% of the cases; presently, no alternative therapies have been shown to give comparable o ... | 2002 | 12150706 |
| hp (2-20) derived from the amino terminal region of helicobacterpylori ribosomal protein l1 exerts its antifungal effects by damaging the plasma membranes of candida albicans. | the fungicidal effects of the peptide hp (2-20). derived from the n-terminal sequence of helicobacter pylori ribosomal protein l1 (rpl1). have been investigated. hp (2-20) displays a strong fungicidal activity against various fungi, without haemolytic activity against human erythrocyte cells, and the fungicidal activity is inhibited by ca2+ and mg2+ ions. in order to investigate the fungicidal mechanism(s) of hp (2-20). the amount of intracellular trehalose was measured in c. albicans. it was fo ... | 2002 | 12212808 |
| microbial agents associated with waterborne diseases. | many classes of pathogens excreted in feces are able to initiate waterborne infections. there are bacterial pathogens, including enteric and aquatic bacteria, enteric viruses, and enteric protozoa, which are strongly resistant in the water environment and to most disinfectants. the infection dose of viral and protozoan agents is lower than bacteria, in the range of one to ten infectious units or oocysts. waterborne outbreaks of bacterial origin (particularly typhoid fever) in the developing coun ... | 2002 | 12546197 |
| increased in-vitro and in-vivo biological activity of lipopolysaccharide extracted from clinical low virulence vaca genotype helicobacter pylori strains. | helicobacter pylori infection in man is associated with chronic gastritis and peptic ulcer disease. the virulence factors of the species are still under investigation. among these, the lipopolysaccharide (lps) is a potential pathogenic factor of the micro-organism, whose biological activity can be estimated by immunological parameters. the aim of this study was to determine the ability of pure lps extracted from clinical isolates of h. pylori to induce mitogenicity, secretion of tumour necrosis ... | 2002 | 12358068 |
| numerical chromosomal abnormality in gastric malt lymphoma and diffuse large b-cell lymphoma. | we investigated numerical chromosomal abnormalities, using the fluorescence in situ hybridization (fish) method, in gastric mucosa-associated lymphoid tissue (malt) lymphoma and diffuse large b-cell lymphoma (dlbl). we also compared the histopathological findings, including the presence or absence of helicobacter pylori infection, with the analytical results. | 2002 | 12375141 |
| [helicobacter pylori--introduction and review of research]. | the discovery of helicobacter pylori has revolutionized the pathophysiological and clinical approach to gastric and duodenal ulcer. since the first paper identifying h. pylori was published only 17 years ago, it has been found out that this bacterium causes probably the commonest human infection. numerous papers published so far have confirmed causal relationship between h. pylori infection and gastritis, duodenal ulcer, gastric ulcer and gastric cancer. if any recent achievement in the world of ... | 2002 | 12592806 |
| relationship between gastric ulcer and helicobacter pylori vaca detected in gastric juice using bead-elisa method. | vaca is an important pathogenetic factor produced by helicobacter pylori. vaca has often been detected in supernatants of liquid cultures or lysates of whole bacterial cells. however, no studies have ever tried to assay vaca produced in the human stomach. we applied a very sensitive and simple method, bead-elisa, to detect vaca in gastric juice. | 2002 | 12390207 |
| [probiotics: history, definition, requirements and possible therapeutic applications]. | the ingestion of probiotics is associated with various beneficial effects on human health and modifies the physiological homeostasis of the intestinal flora. probiotics are microorganisms with some particular characteristics: human origin, safety in human use, bile and acid resistance, survival in the intestine, at least temporary colonization of the human gut, adhesion to the mucosa and bacteriocine production. thanks to these characteristics, probiotics block the invasion of human intestinal c ... | 2002 | 12402663 |
| cag pathogenicity island-specific responses of gastric epithelial cells to helicobacter pylori infection. | helicobacter pylori infects over half the world's population and causes a wide range of diseases, including gastritis, peptic ulcer, and two forms of gastric cancer. h. pylori infection elicits a variety of phenotypic responses in cultured gastric epithelial cells, including the expression of proinflammatory genes and changes in the actin cytoskeleton. both of these responses are mediated by the type iv secretion system (tfss) encoded by the cag pathogenicity island (cag pai). we used human cdna ... | 2002 | 12411577 |
| responses of endoscopy patients in ladakh, india, to helicobacter pylori whole-cell and cag a antigens. | although helicobacter pylori is a cosmopolitan colonizer of the human stomach, the responses among persons in remote populations from whom h. pylori was cultured have not been studied. we report on studies of 189 persons in the ladakh region of india in whom serum immunoglobulin g responses to h. pylori whole-cell and cag a antigens were measured. h. pylori was isolated from 68 of these patients. an h. pylori whole-cell antigen derived from ladakhi strains outperformed a similar antigen from u.s ... | 2002 | 12414766 |
| grb2 is a key mediator of helicobacter pylori caga protein activities. | caga delivered from helicobacter pylori into gastric epithelial cells undergoes tyrosine phosphorylation and induces host cell morphological changes. here we show that caga can interact with grb2 both in vitro and in vivo, which results in the activation of the ras/mek/erk pathway and leads to cell scattering as well as proliferation. importantly, this ability of caga is independent from the tyrosine phosphorylation, which occurs within the five repeated epiya sequences (py region) of caga. howe ... | 2002 | 12419219 |
| comparative analysis of helicobacter dnas and biliary pathology in patients with and without hepatobiliary cancer. | several helicobacter species have recently been isolated from the bile and hepatobiliary systems of murine species, and are well recognized as a pathogen of the hepatobiliary disorder. this study was planned to investigate whether helicobacter species possess a causative potential for human hepatobiliary disease, especially for hepatobiliary carcinogenesis. bile and hepatobiliary tissue samples from 19 patients with hepatobiliary cancer and 19 patients with benign biliary diseases were subjected ... | 2002 | 12419842 |
| helicobacter pylori releases a factor(s) inhibiting cell cycle progression of human gastric cell lines by affecting cyclin e/cdk2 kinase activity and rb protein phosphorylation through enhanced p27(kip1) protein expression. | helicobacter pylori, the main cause of chronic gastritis, plays a central role in the etiology of peptic ulcer disease and gastric cancer. in vitro studies have shown that h. pylori increases gastric epithelial cell turnover, thus increasing the risk for the development of neoplastic clones. the mechanisms by which h. pylori promotes perturbation of cell proliferation are not yet elucidated. to investigate whether products released by h. pylori in culture media interfere with cell cycle progress ... | 2002 | 12441136 |
| the vacuolating toxin of helicobacter pylori mimicks the cftr-mediated chloride conductance. | cystic fibrosis (cf) is caused by defects of the cf transmembrane conductance regulator (cftr), which acts both as an anion-selective channel and as a regulator of other proteins. the relative contribution of these two functions in cf disease is debated. the toxin vaca forms channels with properties similar to those of the cftr, and we report here that it can insert into the membrane of various cells originating from respiratory epithelia, generating a chloride conductance comparable to that pro ... | 2002 | 12459497 |
| involvement of tnf-alpha changes in human cancer development, prevention and palliative care. | cancer development and ageing are complex sciences. from the study on the process of rodent carcinogenesis, we identified tumor necrosis factor-alpha (tnf-alpha) as an important mediator of cancer development. this paper presents three clinical examples of tnf-alpha up-regulation: by cord factors of mycobacterium tuberculosis, such as trehalose 6-monomycolate, as an activator of protein kinase c and by a cord factor like fraction of microsporum canis obtained in the air inside houses in thailand ... | 2002 | 12470903 |
| review article: natural history and epidemiology of helicobacter pylori infection. | helicobacter pylori is a common bacterium infecting about half the world's population. it is causally linked with a diverse spectrum of gastrointestinal clinical disorders including peptic ulcer disease, gastric cancer, and gastric malt lymphoma. the principal reservoir is the human stomach, and transmission probably occurs by person-to-person passage. prevalence rates are generally much higher in developing countries compared to developed countries, although there are subgroups within many regi ... | 2002 | 11849122 |
| critical role of an endogenous gastric peroxidase in controlling oxidative damage in h. pylori-mediated and nonmediated gastric ulcer. | the objective of the present study is to delineate the mechanism of oxidative damage in human gastric ulcerated mucosa despite the presence of some antioxidant enzymes. we report for the first time the critical role of an endogenous peroxidase, a major h(2)o(2) metabolizing enzyme, in controlling oxidative damage in gastric mucosa. human gastric mucosa contains a highly active peroxidase in addition to the myeloperoxidase contributed by neutrophil. in both non-helicobacter pylori (h. pylori)- an ... | 2002 | 11937299 |
| bacterial secrets of secretion: euroconference on the biology of type iv secretion processes. | type iv secretion systems (tfss) mediate secretion or direct cell-to-cell transfer of virulence factors (proteins or protein-dna complexes) from many gram-negative animal, human and plant pathogens, such as agrobacterium tumefaciens, bartonella tribocorum, bordetella pertussis, brucella suis, helicobacter pylori, legionella pneumophila and rickettsia prowazekii, into eukaryotic cells. bacterial conjugation is also classified as a tfss-like process mediating the spread of broad-host-range plasmid ... | 2002 | 11918819 |
| differential glycosylation of mhc class ii molecules on gastric epithelial cells: implications in local immune responses. | class ii major histocompatibility complex (mhc) expression is a hallmark of antigen presenting cells (apc). human gastric epithelial cells (gec) express class ii mhc and this expression increases during infection with helicobacter pylori as does the number of cd4 t cells found adjacent or in between epithelial cells. these observations suggested that human gec act as apcs. to characterize and compare class ii mhc complexes with those present in conventional apc, immunoprecipitated class ii mhc f ... | 2002 | 11975982 |
| reversibility of heterotopic proliferative glands in glandular stomach of helicobacter pylori-infected mongolian gerbils on eradication. | helicobacter pylori (hp) infection is an important factor in human gastric disorders. mongolian gerbils can be easily infected with hp and represent excellent experimental models to clarify the role of hp in chronic active gastritis, peptic ulcers, intestinal metaplasia, and gastric carcinoma. we have proved the enhancing effects of hp infection on all histological types of gastric cancers in mongolian gerbils exposed to chemical carcinogens. heterotopic proliferative glands (hpgs) also frequent ... | 2002 | 11985786 |
| association analysis of polymorphisms at the interleukin-1 locus in essential hypertension. | infection with microorganisms such as helicobacter pylori and chlamydia pneumoniae has been associated with coronary heart disease (cad) and hypertension (ht). infection increases the release of pro-inflammatory cytokines, thus facilitating interactions that lead to vascular damage and other effects. we hypothesized that genetically determined differences in activity or responsiveness of cytokine(s) might contribute to ht. the interleukin-1 gene (il1) cluster on chromosome 2q14 contains three re ... | 2002 | 11840488 |
| correlation between helicobacter pylori infection and il-18 mrna expression in human gastric biopsy specimens. | our data indicate that h. pylori infection is associated with active interleukin-18 production in patients with chronic gastritis. different cell types appear to be involved in this activity and may play a role in the development of immunopathologic damage. | 2002 | 12095959 |
| effect of mannitol on helicobacter pylori-induced cyclooxygenase-2 expression in gastric epithelial ags cells. | oxygen radicals have been considered to be important regulatory molecules in helicobacter pylori-induced gastric ulceration and carcinogenesis. h. pylori-induced inflammation has been shown to be associated with cyclooxygenase-2 (cox-2) expression in experimental animals and human patients. this study aimed to determine if h. pylori produces oxygen radicals and induces cox-2 expression in gastric epithelial cells. a further aim was to resolve whether or not the h. pylori-induced cox-2 expression ... | 2002 | 12393940 |
| interleukin 1beta polymorphisms increase risk of hypochlorhydria and atrophic gastritis and reduce risk of duodenal ulcer recurrence in japan. | interleukin-1 beta (il-1beta) polymorphisms are associated with increased risk of gastric cancer in whites. this study aimed to examine effects of these polymorphisms on gastric acid secretion, atrophic gastritis, and risk of peptic ulcer in japan. | 2002 | 12105837 |
| analysis of p53 mutations and helicobacter pylori infection in human and animal models. | p53 gene mutations are believed to play a critical role in the development of gastric carcinoma. we examined the relation between helicobacter pylori infection and p53 gene mutations of the gastric mucosa in human and animal models. | 2002 | 12109656 |
| helicobacter pylori-induced activation of human endothelial cells. | helicobacter pylori infection causes active chronic inflammation with a continuous recruitment of neutrophils to the inflamed gastric mucosa. to evaluate the role of endothelial cells in this process, we have examined adhesion molecule expression and chemokine and cytokine production from human umbilical vein endothelial cells stimulated with well-characterized h. pylori strains as well as purified proteins. our results indicate that endothelial cells actively contribute to neutrophil recruitmen ... | 2002 | 12117971 |
| randomized open trial for comparison of proton pump inhibitors in triple therapy for helicobacter pylori infection in relation to cyp2c19 genotype. | genetic polymorphism of cytochrome p450 (cyp) 2c19 influences the efficacy of helicobacter pylori eradication therapy with a proton pump inhibitor (ppi) and amoxicillin. however, in triple therapy (ppi plus amoxicillin and clarithromycin), little is known about the impact of cyp2c19 polymorphism, or the use of rabeprazole, which is not well metabolized by cyp2c19. the efficacy of three ppi (omeprazole, lansoprazole, and rabeprazole) in a 1-week triple regimen were compared in relation to cyp2c19 ... | 2002 | 12121503 |
| [association between helicobacter pylori caga strain infection and expression of cyclooxygenase 2 in gastric carcinoma]. | to observe the expression of cyclooxygenase 2 (cox-2) in tissues of human gastric cancer and explore the association between helicobacter pylori caga strain infection and the expression of cox-2 so as to provide a theoretical basis for early prevention of gastric cancer. | 2002 | 12126507 |
| cancer prevention and therapy with kiwifruit in chinese folklore medicine: a study of kiwifruit extracts. | kiwi gold fruits were extracted successively with hexane, acetone, methanol and 70% methanol, and further fractionated by silica gel and ods column chromatographies for the assays of various biological activities. five fractions h1, h2 (hexane extract), al, a2 (acetone extract) and m2 (methanol extract) showed selective cytotoxic activity against human oral tumor cell lines, which was more sensitive than human gingival fibroblasts. more hydrophilic fractions [70m3, 70m4, 70m5] of 70% methanol ex ... | 2002 | 12127237 |
| helicobacter pylori caga protein activates serum response element-driven transcription independently of tyrosine phosphorylation. | infection with helicobacter pylori possessing the cag pathogenicity island (pai) is associated with severe gastritis and gastric cancer. caga protein, one of the products of cag pai, is translocated into epithelial cells, where cytoskeletal rearrangements occur as a result of caga tyrosine (tyr) phosphorylation. here we identify a new role for caga protein as an activator of host cell signaling. | 2002 | 12454853 |
| heat shock protein of mycoplasma salivarium and mycoplasma orale strains isolated from hiv-seropositive patients. | it has been suggested that infection by some mycoplasma species can act as possible cofactors in the acceleration of immunodeficiency in hiv-infected patients. the present study was designed to examine infections by oral mycoplasma species in hiv-seropositive (hiv(+)) patients. mycoplasma salivarium and mycoplasma orale were isolated from 59.5% and 16.7% of 42 hiv(+) patients, respectively. non-m. salivarium and non-m. orale species were isolated from 40.5% of saliva samples from the hiv(+) grou ... | 2002 | 12687728 |
| src is the kinase of the helicobacter pylori caga protein in vitro and in vivo. | the gastric pathogen helicobacter pylori uses a type iv secretion system to inject the bacterial caga protein into gastric epithelial cells. within the host cell, caga becomes phosphorylated on tyrosine residues and initiates cytoskeletal rearrangements. we demonstrate here that src-like protein-tyrosine kinases mediate caga phosphorylation in vitro and in vivo. first, the src-specific tyrosine kinase inhibitor pp2 specifically blocks caga phosphorylation and cytoskeletal rearrangements thereby ... | 2002 | 11788577 |
| removal of mucus for ultrastructural observation of the surface of human gastric epithelium using pronase. | helicobacter pylori adhering to the human gastric epithelium causes gastric diseases such as ulcer, carcinoma and lymphoma. it is thus important to observe in detail both the surface of the epithelial cells and the h. pylori that adhered to it for the elucidation of h. pylori-induced diseases by scanning electron microscopy (sem). since the thick mucus layer blocks the observation of the cell surface and the bacteria, it is generally eliminated during the processing for sem by roughly mechanical ... | 2002 | 11966871 |
| effect of cold starvation, acid stress, and nutrients on metabolic activity of helicobacter pylori. | helicobacter pylori can transform, in vivo as well as in vitro, from dividing spiral-shaped forms into nonculturable coccoids, with intermediate forms called u forms. the importance of nonculturable coccoid forms of h. pylori in disease transmission and antibiotic treatment failures is unclear. metabolic activities of actively growing as well as nonculturable h. pylori were investigated by comparing the concentrations of cellular atp and total rna, gene expression, presence of cytoplasmic polyph ... | 2002 | 11772603 |
| identification of novel immunogenic proteins of helicobacter pylori by proteome technology. | cell surface proteins of the human gastric pathogen helicobacter pylori, reference strain ccug 17874, were extracted with acid glycine and fractionated by heparin affinity chromatography. the extracts were subsequently analysed using high-resolution two-dimensional gel electrophoresis (2-de) and immunoblotting. four proteins of low molecular masses (25-30 kda) stained by coomassie r-350, were identified by peptide esi-ms/ms sequencing after in-gel tryptic digestion. the identified proteins were ... | 2002 | 11730836 |