Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
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| topology and membrane interaction of helicobacter pylori comb proteins involved in natural transformation competence. | the human gastric pathogen helicobacter pylori is naturally competent for genetic transformation. the h. pylori comb gene duster encodes the virb4-homologous atpase comb4 and the structural proteins comb7-comb10, which share significant sequence identity to the agrobacterium tumefaciens virb-encoded type iv secretion system. to study the topology of the comb7-10 proteins, we applied tnmax transposon mutagenesis by generating fusions of comb proteins with mature beta-lactamase (blam) or alkaline ... | 2003 | 12868652 |
| idiopathic interstitial pneumonia following stem cell transplantation. | idiopathic interstitial pneumonia (iip) can occur after stem cell transplantation, but the aetiology is unknown. based on the association between angiitis syndrome and helicobacter pylori infection, we identified possible risk factors common to these two conditions. among 83 patients who underwent stem cell transplantation, four developed iip. we elucidated various parameters and clinical features in four patients with iip and 79 patients without, after allogeneic stem cell transplantation. in a ... | 2003 | 12868990 |
| occurrence of a nontypable helicobacter pylori strain lacking lewis blood group o antigens and dd-heptoglycan: evidence for the role of the core alpha1,6-glucan chain in colonization. | the cell envelope of helicobacter pylori contains a lipopolysaccharide (lps) essential for the physical integrity and functioning of the bacterial cell membrane. the o-chain of this lps frequently expresses type 2 lewis x (lex) and lewis y (ley) blood group antigens that mimic human gastric mucosal cell-surface glycoconjugates. this article describes the isolation and structural analysis of the lps from a clinical isolate of h. pylori strain pj2 that lacks le antigens but is still capable of col ... | 2003 | 12907691 |
| recent advances in helicobacter pylori infection in children: from the petri dish to the playground. | helicobacter pylori infection is acquired in childhood, plays a causative role in chronic gastritis and peptic ulcer disease, and is associated with the development of gastric cancer. the present review focuses on recent advances in the scientific knowledge of h pylori infection in children, including clinical sequelae, diagnosis and treatment. in addition, recent insights regarding both bacterial and host factors that mediate human diseases associated with h pylori infection are discussed. | 2003 | 12915920 |
| human leukocyte antigen class ii genes and helicobacter pylori infection: does genotype overwhelm environmental exposure? | we investigated associations between human leukocyte antigen class ii genes, environmental exposures, and helicobacter pylori infection. | 2003 | 12921878 |
| review article: clinical significance of mucosal-protective agents: acid, inflammation, carcinogenesis and rebamipide. | while a great deal of clinical evidence has been found regarding anti-acids for the treatment of gastric disorders including peptic ulcers, not all disorders can be explained only by the hyperfunction of acid secretion. especially in the asian region, glandular atrophy is more prominent than in western countries, therefore low acid output is often observed in these patients. improvement of mucosal protection is rational therapy for these patients; this is the reason for use of these agents in as ... | 2003 | 12925154 |
| evaluation of helicobacter species in inflammatory bowel disease. | bacteria have been implicated in the pathogenesis of inflammatory bowel disease. helicobacter species have been shown to cause colitis in animal models and have been identified in human diarrhoeal illness and crohn's disease. | 2003 | 12950420 |
| treatment of helicobacter pylori infection using a novel antiadhesion compound (3'sialyllactose sodium salt). a double blind, placebo-controlled clinical study. | 3' sialyllactose sodium salt (3'sl) is an oligosaccharide that occurs naturally in human and bovine milk. it can inhibit the adhesion of h. pylori to human epithelial cells in vitro. the aim of this study was to test whether this oligosaccharide can suppress or cure h. pylori colonization in vivo and to determine its safety in humans. | 2003 | 12950597 |
| [persistence factors of helicobacter pylori]. | isolates of h. pylori from patients with chronic gastritis and peptic ulcer, were found to be capable of inactivating lysozyme and intercide (the bactericidal component of human leukocytic interferon). the expression and penetration capacity of their antilysozyme activity (ala) and antiintercide activity was determined. the wide spread of ala among h. pylori clinical isolates associated with inflammatory changes in bioptic specimens, confirmed the leading role of this microorganism in the pathog ... | 2003 | 12966868 |
| cytoskeletal rearrangements in gastric epithelial cells in response to helicobacter pylori infection. | helicobacter pylori causes host epithelial cell cytoskeletal rearrangements mediated by the translocation and tyrosine phosphorylation of an outer-membrane protein, caga, and by the vacuolating cytotoxin, vaca. however, the mechanisms by which h. pylori mediates cytoskeletal rearrangements in infected host cells need to be more clearly defined. the aim of this study was to determine the effects of h. pylori isolates from children on the architecture of host gastric epithelial cells. gastric epit ... | 2003 | 12972579 |
| acid-adaptive genes of helicobacter pylori. | helicobacter pylori is the only neutralophile that has been able to colonize the human stomach by using a variety of acid-adaptive mechanisms. one of the adaptive mechanisms is increased buffering due to expression of an acid-activated inner membrane urea channel, urei, and a neutral ph-optimum intrabacterial urease. to delineate other possible adaptive mechanisms, changes in gene expression in response to acid exposure were examined using genomic microarrays of h. pylori exposed to different le ... | 2003 | 14500513 |
| t(11;18)(q21;q21) in gastric malt lymphoma and diffuse large b-cell lymphoma of chinese patients. | t(11;18)(q21;q21) occurs at variable frequencies in mucosa-associated lymphoid tissue (malt) lymphomas of different sites. in gastric malt lymphoma, it occurs in 30% cases and the translocation positive tumours are frequently at advanced stages and do not respond to helicobacter pylori eradication. intriguingly, t(11;18)(q21;q21) has been observed only rarely in mucosal diffuse large b-cell lymphomas (dlbcl), despite that at least a proportion of these tumours are believed to be the result of tr ... | 2003 | 14502259 |
| cutting edge: cyclooxygenase-2 activation suppresses th1 polarization in response to helicobacter pylori. | helicobacter pylori infection causes a th1-driven mucosal immune response. cyclooxygenase (cox)-2 is up-regulated in lamina propria mononuclear cells in h. pylori gastritis. because cox-2 can modulate th1/th2 balance, we determined whether h. pylori activates cox-2 in human pbmcs, and the effect on cytokine and proliferative responses. there was significant up-regulation of cox-2 mrna and pge(2) release in response to h. pylori preparations. addition of cox-2 inhibitors or an anti-pge(2) ab resu ... | 2003 | 14530307 |
| human beta-defensin-2 induction in helicobacter pylori-infected gastric mucosal tissues: antimicrobial effect of overexpression. | the objective of this study was to understand more of the innate immune response to helicobacter pylori by determining the expression of human beta-defensin-2 (hbd-2) in various gastric mucosal tissues and mkn45 gastric cancer cells with or without h. pylori. semi-quantitative taqman rt-pcr and immunohistochemistry were carried out. the antimicrobial effects of a transfected hbd-2 gene against h. pylori were also evaluated. the results showed that hbd-2 was expressed in inflamed gastric mucosal ... | 2003 | 12488564 |
| chemokine receptor 5 expression in gastric mucosa of helicobacter pylori-infected and noninfected children. | experimental data from human adults or animal models indicate that the helicobacter pylori-specific immune response is dominated by inflammatory t cells of the th1 type. to investigate whether a th1 immune response is established in early h. pylori infection, gastric biopsy samples from 70 children were subjected to immunohistochemical analysis. to this end, t cells, b cells, monocytes, neutrophils, and chemokine receptor 5 (ccr5)-expressing (ccr5(+)) cells, which are associated with th1 immune ... | 2003 | 12522035 |
| decreased epithelial cytokine responses in the duodenal mucosa of helicobacter pylori-infected duodenal ulcer patients. | helicobacter pylori colonizes the human stomach and areas of gastric metaplasia in the duodenum, but only a minority of those that are infected develop symptoms, e.g., peptic ulcers. although most ulcers occur in the duodenum, almost all studies of mucosal immune responses against the infection have been limited to responses in the stomach. in the present study we evaluated whether there are differences in the levels of proinflammatory cytokines as well as immunoregulatory cytokines in the duode ... | 2003 | 12522049 |
| association of cyclooxygenase-2 expression with hp-caga infection in gastric cancer. | to observe the expression of cyclooxygenase-2 (cox-2) and to investigate the association between cox-2 expression and infection with cytotoxic-associated gene a (caga) positive strain helicobacter pylori (hp) in human gastric cancer, and subsequently to provide fresh ideas for the early prevention of gastric cancer. | 2003 | 12532440 |
| multiple infections and subsequent cardiovascular events in the heart outcomes prevention evaluation (hope) study. | limited prospective epidemiological data are available on the relation between exposure to chlamydia pneumoniae, helicobacter pylori, cytomegalovirus (cmv), and hepatitis a virus (hav), individually or as a total pathogen score, and human cardiovascular (cv) disease. | 2003 | 12538424 |
| gastric epithelial reactive oxygen species prevent normoxic degradation of hypoxia-inducible factor-1alpha in gastric cancer cells. | the expression of hypoxia inducible factor (hif)-1alpha protein is tightly regulated by cellular oxygen status. namely, hif-1alpha protein is degraded rapidly in normoxic cells, whereas hypoxia stabilizes hif-1alpha to transactivate hypoxia-responsive genes. here we show that hif-1alpha protein is expressed aberrantly in gastric cancer cells under normoxia in a reactive oxygen species (ros)-dependent manner. the normoxic expression of hif-1alpha in concordance with its dna binding activity enhan ... | 2003 | 12538497 |
| impact of clarithromycin resistance and cyp2c19 genetic polymorphism on treatment efficacy of helicobacter pylori infection with lansoprazole- or rabeprazole-based triple therapy in japan. | helicobacter pylori treatment failure is thought to be due mainly to polymorphic cytochrome p450 2c19 (cpy2c19) genetic polymorphism, associated with proton pump inhibitor metabolism, and antimicrobial susceptibility. this report has ascertained which was more important, cpy2c19 polymorphism or antimicrobial susceptibility, when using 1-week lansoprazole-based or rabeprazole-based triple therapy in japan. | 2003 | 12544691 |
| catalase (kata) and kata-associated protein (kapa) are essential to persistent colonization in the helicobacter pylori ss1 mouse model. | helicobacter pylori infects the human gastric mucosa and elicits an aggressive inflammatory response. despite the severity of the inflammatory response, the bacterium is able to persist and cause a chronic infection. it is believed that antioxidant defence mechanisms enable this organism to persist. wild-type h. pylori strain ss1, and kata- and kapa-deficient mutants, were used to infect c57/bl6 mice to test this hypothesis. neither kata nor kapa was essential for the initial colonization of h. ... | 2003 | 12634335 |
| cloning, expression and purification of three chaperonin 60 homologues. | the chaperonin 60 (cpn60) proteins have, in addition to their well-known functions of protein folding and protection, a range of intercellular signalling activities. as part of a study to investigate the biological activity of the cpn60 proteins, particularly from pathogenic organisms, we have cloned and expressed three cpn60 proteins from homo sapiens, helicobacter pylori and chlamydia pneumoniae. the cpn60 proteins were purified to apparent homogeneity using a combination of nickel column affi ... | 2003 | 12651007 |
| mutant p53 expression and apoptotic activity of helicobacter pylori positive and negative gastritis in correlation with the presence of intestinal metaplasia. | mutation of the p53 gene is detectable in most cases of gastric cancer, as it is the most common genetic alteration in human malignancies. it is also well documented that helicobacter pylori infection plays an important role in gastric carcinogenesis. there is still no clarification, however, concerning how genetic instability influences the homeostasis of gastric epithelium. we have studied the effect of h. pylori infection on apoptosis of the antral epithelium in the presence/absence of intest ... | 2003 | 12655259 |
| molecular cloning of p53 cdna of mongolian gerbil and establishment of yeast p53 functional assay system. | epidemiological studies have shown a correlation between helicobacter pylori infection and human gastric carcinogenesis. a mongolian gerbil model has demonstrated that h. pylori infection induced gastric carcinoma. however, the disadvantage of this animal model is a lack of information regarding the cellular genes involved in oncogenesis. mutation of the p53 gene is one of the most common steps in gastric carcinogenesis. in this study, we aimed to clone the p53 gene of the mongolian gerbil and d ... | 2003 | 12662374 |
| lafutidine, a novel histamine h2-receptor antagonist, vs lansoprazole in combination with amoxicillin and clarithromycin for eradication of helicobacter pylori. | in contrast to the growing amount of data concerning proton pump inhibitor-based triple therapy for helicobacter pylori infection, it is still controversial whether proton pump inhibitor can be replaced by h2 receptor antagonist without compromising efficacy. lafutidine is a novel potent h2 receptor antagonist with gastroprotective activities such as enhancement of gastric mucosal blood flow. | 2003 | 12662378 |
| the neutrophil-activating protein of helicobacter pylori (hp-nap) activates the mapk pathway in human neutrophils. | infection by helicobacter pylori causes an acute inflammatory response followed by a chronic infection of the human gastric mucosa characterized by the infiltration of neutrophils andmononuclear inflammatory cells. the neutrophil-activating protein of helicobacter pylori (hp-nap) is a virulence factor that activates neutrophils, monocytes, and mast cells. however, the mechanism by which hp-nap activates these cells is not fully understood. here, we show that hp-nap induces extracellular regulate ... | 2003 | 12672049 |
| influence of gastrin on the expression of cyclooxygenase-2, hepatocyte growth factor and apoptosis-related proteins in gastric epithelial cells. | several studies have shown a link between gastrin and gastric cancer, both in humans and animals, especially infected with helicobacter pylori (h. pylori). however, the exact role of hypergastrinemia in gastric carcinogenesis remains still undetermined. the aim of the present study was to evaluate the interaction between gastrin, cyclooxygenase-2 (cox-2), hepatocyte growth factor (hgf) and apoptosis-related proteins (bax, bcl-2, caspase-3, survivin) in cultured gastric epithelial cancer cells. | 2003 | 12674216 |
| variable frequencies of t(11;18)(q21;q21) in malt lymphomas of different sites: significant association with caga strains of h pylori in gastric malt lymphoma. | t(11;18)(q21;q21) is a specific chromosomal translocation associated with mucosa-associated lymphoid tissue (malt) lymphoma. it fuses the amino terminal of the api2 gene to the carboxyl terminal of the malt1 gene and generates a chimeric fusion product. although the translocation is frequently detected in gastric and pulmonary malt lymphoma, its incidence in malt lymphomas from other sites is largely unknown. it also remains unknown whether the occurrence of the translocation is influenced by th ... | 2003 | 12676782 |
| expression cloning of a periodontitis-associated apoptotic effector, cage homologue, in actinobacillus actinomycetemcomitans. | to study anti-bacterial immunity and to identify critical bacterial antigens associated with specific periodontal infection, we screened the genomic library of actinobacillus actinomycetemcomitans, a major gram(-) anaerobe causing human periodontitis, by expression cloning using disease-associated periodontal cd4(+)t cells derived from hupbl-engrafted nod/scid mice. here, we report one of the novel genes identified and designated, cage homologue (in short: cage) of a. actinomycetemcomitans, whic ... | 2003 | 12684047 |
| effect of helicobacter pylori eradicated therapy on water gastric emptying in patients with active duodenal ulcer. | it remains debatable if duodenal ulcer (du) or helicobacter pylori infection has a definite impact on human gastric emptying (ge). we explored the nature of water ge in active du patients before and after ulcer healing and the influence of h. pylori eradication on ge. | 2003 | 14535981 |
| induction of cyclooxygenase 2 by escherichia coli but not helicobacter pylori lipopolysaccharide in gastric epithelial cells in vitro. | cyclooxygenase 2 (cox-2) is an inducible enzyme that plays a key role in the synthesis of prostaglandins in response to inflammatory stimuli. it is expressed in the gastric mucosa as part of the response to infection with helicobacter pylori. the specific interaction between h. pylori and the gastric epithelium that results in cox-2 expression has not been identified. | 2003 | 14535998 |
| the muc5ac glycoprotein is the primary receptor for helicobacter pylori in the human stomach. | helicobacter pylori shows a characteristic tropism for the mucus-producing gastric epithelium. in infected patients, h. pylori colocalizes in situ with the gastric secretory mucin muc5ac. the carbohydrate blood-group antigen lewis b (leb) was deemed responsible for the adherence of h. pylori to the gastric surface epithelium. we sought to determine if muc5ac is the carrier of leb, and thus if muc5ac is the underlying gene product functioning as the main receptor for h. pylori in the stomach. | 2003 | 14535999 |
| inhibition of helicobacter pylori-induced nuclear factor-kappa b activation and interleukin-8 gene expression by ecabet sodium in gastric epithelial cells. | helicobacter pylori stimulates nuclear factor-kappa b (nf-kappa b) activation and chemokine interleukin-8 (il-8) expression in gastric epithelial cells. ecabet sodium (ecabet), a locally acting antiulcer drug, is known to have anti-h. pylori activity. however, there is little understanding of how ecabet induces anti-inflammatory activity in gastric epithelial cells infected with h. pylori. the aim of this study was to investigate the effects of ecabet on il-8 gene expression and nf-kappa b activ ... | 2003 | 14536001 |
| [relationship between helicobacter pylori infection and expression of c-myc, bcl-2, and bax protein in different gastric mucosa lesions]. | helicobacter pylori (hp) has been believed to be a carcinogen of gastric carcinoma. however, its mechanism was yet not clearly understood. this study was designed to investigate the relationship between hp infection and gastric epithelial cell proliferation as well as apoptosis in different gastric mucosa lesions and elucidate the probable mechanism of gastric carcinogenesis relating with hp infection. | 2003 | 14558945 |
| coexpression of helicobacter pylori's proteins caga and hspb induces cell proliferation in ags gastric epithelial cells, independently from the bacterial infection. | adenocarcinoma of the stomach is the second most common cause of cancer mortality in the world. the purpose of this study was to evaluate the potential role in carcinogenesis of two secreted helicobacter pylori's proteins, caga and hspb, both shown to increase the risk of gastric carcinoma in patients infected with h. pylori-positive strain. the effects of these two proteins on cell kinetics and the ability to selectively affect the expression of cell cycle-related proteins by transfection of a ... | 2003 | 14559823 |
| helicobacter pylori: pathogen and symbiont. | there are puzzles in defining the exact role of helicobacter pylori infection in humans. the bacterium, which is associated with human disease of the upper gastrointestinal tract, may otherwise exist as a commensal with probable symbiotic association in some human hosts. although virulence associated genes have been detected in some strains which explain their pathogenicity, their pathogenic effect and subsequent clinical manifestations seem to vary in different human populations or geographical ... | 2003 | 14567051 |
| [disturbance of intracellular signaling by helicobacter pylori infection]. | 2003 | 14577356 | |
| remission of severe anemia persisting for over 20 years after eradication of helicobacter pylori in cases of ménètrier's disease and atrophic gastritis: helicobacter pylori as a pathogenic factor in iron-deficiency anemia. | a man with a 20-year history of recurrent iron-deficiency anemia complicated by helicobacter pylori-positive ménètrier's disease was observed over a 10-year clinical course, during which time he was successfully treated for the anemia and a gastric helicobacter pylori (h. pylori) infection through eradication. considering the satisfactory therapeutic results in this case, we performed eradication therapy on another h. pylori-positive atrophic gastritis case with a 24-year history of iron-deficie ... | 2003 | 14606710 |
| [invasiveness and cytopathogenicity of helicobacter pylori in human cell culture]. | invasive and cytopathogenic properties of clinical strains of helicobacter pylori in the cell culture hep-2 have been studied. it has been established that h. pylori possesses high invasive and adhesive capacity. it has been shown that h. pylori takes considerable effect on the indices of mitotic activity, such as mitotic index (mi), level of pathogenic mitoses; it also stimulates the appearance of pathologies connected with the disturbance of mitotic apparatus and damage of chromosomes. h. pylo ... | 2003 | 14618781 |
| helicobacter pylori-induced homotypic phagosome fusion in human monocytes is independent of the bacterial vaca and cag status. | following reports that a vaca+cag+ toxigenic but not a vaca-cag- non-toxigenic helicobacter pylori strain induced homotypic phagosome fusion in murine macrophages, we addressed that phenomenon in human cells. mononuclear phagocytes and epitheloid cells were challenged with h. pylori strains of different vaca and cag genotypes and with vaca- and cag- isogenic mutants, and chased in the absence or presence of signal transduction modulators. electron microscopy revealed that, in monocytes: (i) homo ... | 2003 | 14641174 |
| effect of omeprazole 10 mg on intragastric ph in three different cyp2c19 genotypes, compared with omeprazole 20 mg and lafutidine 20 mg, a new h2-receptor antagonist. | omeprazole 10 mg is used as maintenance therapy for gastro-oesophageal reflux disease, but previous reports have not mentioned the potency of its acid suppression. | 2003 | 14653835 |
| micropneumatosis coexistent with helicobacter pylori and its improvement. | micropneumatosis intestinalis is the occurrence of gas-filled circular voids with diameter of 20-200 microns, not lined with epithelium. we report a 39-year-old man with superficial gastritis and helicobacter pylori infection who also had gastric, duodenal and colonic micropneumatosis. endoscopic biopsy after treatment for h. pylori gastritis showed no micropneumatosis in gastric, duodenal or colonic mucosal sections. we suggest that h. pylori may be one of the causative factors for micropneumat ... | 2003 | 14658539 |
| association of the presence of helicobacter in gallbladder tissue with cholelithiasis and cholecystitis. | the presence of helicobacter dna species has been investigated in the biliary epithelium of patients with biliary diseases. however, conflicting results have been observed that may have been due to the small number of subjects studied, difficulty in obtaining a healthy control group, absence of controlling for confounding factors, or differences among populations. therefore, we investigated the presence of helicobacter species by culture and nested pcr of 16s rrna genes in gallbladder tissue and ... | 2003 | 14662950 |
| api2-malt1 fusion gene in colorectal lymphoma. | the api2-malt1 fusion gene was originally identified from a t(11;18)(q21;q21) translocation, a specific chromosomal abnormality that is found in mucosa-associated lymphoid tissue (malt) lymphoma. gastric malt lymphomas positive for the api2-malt1 fusion gene do not respond to helicobacter pylori-eradication therapy, but otherwise, the incidence and clinicopathological behavior of colorectal malt lymphoma with this genetic abnormality are unclear. we examined the api2-malt1 fusion by multiplex rt ... | 2003 | 14681324 |
| high-dose rabeprazole/amoxicillin therapy as the second-line regimen after failure to eradicate h. pylori by triple therapy with the usual doses of a proton pump inhibitor, clarithromycin and amoxicillin. | some patients are refractory to the usual triple therapy for eradication of helicobacter pylori, consisting of a proton pump inhibitor, amoxicillin and clarithromycin, so there needs to be an alternative strategy for retreatment after failure to eradicate the infection. | 2003 | 14696516 |
| is intestinal metaplasia of the stomach reversible? | intestinal metaplasia (im) of the stomach is a risk factor in developing intestinal-type gastric cancer and hence the question of reversibility is vital. there is emerging epidemiological evidence that with long term follow up, im may be reversible although a combination of antioxidant agents and eradication of h pylori may be necessary to achieve this. the pathogenesis of im is currently being elucidated and it is likely that a combination of bacterial, host, and environmental factors will be s ... | 2003 | 12477745 |
| plural transformation-processes from spiral to coccoid helicobacter pylori and its viability. | helicobacter pylori (h. pylori), present in a half of the world's population, is a very successful pathogen. the infection by this bacterium causes several gastric diseases in human. h. pylori is morphologically divided into two types; a spiral and a coccoid form. both types are observed in human stomach. although the former is converted into the latter in vitro, the process of coccoid formation remains obscure. furthermore, whether coccoid forms possess viability arouses much controversy among ... | 2003 | 12504609 |
| expression of lewis(b) blood group antigen in helicobacter pylori does not interfere with bacterial adhesion property. | the finding that some helicobacter pylori strains express lewis b (le(b)) blood group antigen casts a doubt on the role of le(b) of human gastric epithelium being a receptor for h. pylori. the aim of this study was to determine if expression of le(b) in h. pylori interferes with bacterial adhesion property. | 2003 | 12508365 |
| [relationship between helicobacter pylori infection and expressions of tumor suppressor genes in gastric carcinoma and related lesions]. | helicobacter pylori (hp) are believed to be a carcinogen of gastric carcinoma. however, its mechanism was yet not clearly understood. p53, p21waf1, and p16 are main negative regulator genes of cell cycle. this study was designed to investigate the relationship between these 3 genes and hp infection. | 2002 | 12508544 |
| helicobacter pylori organisms induce expression of activation and apoptotic surface markers on human lymphocytes and ags cells: a cytofluorimetric evaluation. | human peripheral blood mononuclear cells (pbmcs) were treated with helicobacter pylori (hp) organisms alone or with hp-stimulated ags cells (a gastric adenocarcinoma cell line). hp organisms were able per se to increase the percentage of cd8 +/- cd95 +/- cells, while number of cd25+ cells and hla-dr molecule expression increased following pretreatment with hp-stimulated ags cells. a comparison was made with a test system in which pbmcs were stimulated with escherichia coli (ec) organisms and col ... | 2002 | 12510791 |
| transcriptional regulation by thiol compounds in helicobacter pylori-induced interleukin-8 production in human gastric epithelial cells. | reactive oxygen species (ros) have been counted among the potential toxic factors involving helicobacter pylori (h. pylori)-induced gastric injury. transcription nuclear factor-kappab (nf-kappab) is activated by ros and regulates inflammatory gene expression. thiol compounds, such as glutathione and n-acetylcysteine, scavenge hydrogen peroxide and are reported to prevent oxidative damage in various cells. the present study aims to investigate whether thiol compounds could affect h. pylori-induce ... | 2002 | 12485925 |
| identification of helicobacter pylori dna in human cholesterol gallstones. | the gallbladder mucosa secretes hydrogen ions and is covered by mucus. the environmental conditions for bacterial colonization are similar to those in the stomach. gallbladder stones often contain dna from enteric bacteria, but no compelling evidence demonstrates that helicobacter spp. have been present. the aim of this study was to establish bacterial dna profiles in cholesterol gallstones with special reference to helicobacter pylori. | 2002 | 11843027 |
| hp (2-20) derived from the amino terminal region of helicobacterpylori ribosomal protein l1 exerts its antifungal effects by damaging the plasma membranes of candida albicans. | the fungicidal effects of the peptide hp (2-20). derived from the n-terminal sequence of helicobacter pylori ribosomal protein l1 (rpl1). have been investigated. hp (2-20) displays a strong fungicidal activity against various fungi, without haemolytic activity against human erythrocyte cells, and the fungicidal activity is inhibited by ca2+ and mg2+ ions. in order to investigate the fungicidal mechanism(s) of hp (2-20). the amount of intracellular trehalose was measured in c. albicans. it was fo ... | 2002 | 12212808 |
| effects of rabeprazole, lansoprazole and omeprazole on intragastric ph in cyp2c19 extensive metabolizers. | to investigate the inhibitory effects on gastric acid secretion of three proton pump inhibitors, omeprazole, lansoprazole and rabeprazole, using a three-way crossover design in healthy helicobacter pylori-negative,s-mephenytoin 4'-hydroxylase (cyp2c19) homo- and hetero-extensive metabolizers. | 2002 | 12269976 |
| increased in-vitro and in-vivo biological activity of lipopolysaccharide extracted from clinical low virulence vaca genotype helicobacter pylori strains. | helicobacter pylori infection in man is associated with chronic gastritis and peptic ulcer disease. the virulence factors of the species are still under investigation. among these, the lipopolysaccharide (lps) is a potential pathogenic factor of the micro-organism, whose biological activity can be estimated by immunological parameters. the aim of this study was to determine the ability of pure lps extracted from clinical isolates of h. pylori to induce mitogenicity, secretion of tumour necrosis ... | 2002 | 12358068 |
| numerical chromosomal abnormality in gastric malt lymphoma and diffuse large b-cell lymphoma. | we investigated numerical chromosomal abnormalities, using the fluorescence in situ hybridization (fish) method, in gastric mucosa-associated lymphoid tissue (malt) lymphoma and diffuse large b-cell lymphoma (dlbl). we also compared the histopathological findings, including the presence or absence of helicobacter pylori infection, with the analytical results. | 2002 | 12375141 |
| relationship between gastric ulcer and helicobacter pylori vaca detected in gastric juice using bead-elisa method. | vaca is an important pathogenetic factor produced by helicobacter pylori. vaca has often been detected in supernatants of liquid cultures or lysates of whole bacterial cells. however, no studies have ever tried to assay vaca produced in the human stomach. we applied a very sensitive and simple method, bead-elisa, to detect vaca in gastric juice. | 2002 | 12390207 |
| effect of mannitol on helicobacter pylori-induced cyclooxygenase-2 expression in gastric epithelial ags cells. | oxygen radicals have been considered to be important regulatory molecules in helicobacter pylori-induced gastric ulceration and carcinogenesis. h. pylori-induced inflammation has been shown to be associated with cyclooxygenase-2 (cox-2) expression in experimental animals and human patients. this study aimed to determine if h. pylori produces oxygen radicals and induces cox-2 expression in gastric epithelial cells. a further aim was to resolve whether or not the h. pylori-induced cox-2 expression ... | 2002 | 12393940 |
| [probiotics: history, definition, requirements and possible therapeutic applications]. | the ingestion of probiotics is associated with various beneficial effects on human health and modifies the physiological homeostasis of the intestinal flora. probiotics are microorganisms with some particular characteristics: human origin, safety in human use, bile and acid resistance, survival in the intestine, at least temporary colonization of the human gut, adhesion to the mucosa and bacteriocine production. thanks to these characteristics, probiotics block the invasion of human intestinal c ... | 2002 | 12402663 |
| cag pathogenicity island-specific responses of gastric epithelial cells to helicobacter pylori infection. | helicobacter pylori infects over half the world's population and causes a wide range of diseases, including gastritis, peptic ulcer, and two forms of gastric cancer. h. pylori infection elicits a variety of phenotypic responses in cultured gastric epithelial cells, including the expression of proinflammatory genes and changes in the actin cytoskeleton. both of these responses are mediated by the type iv secretion system (tfss) encoded by the cag pathogenicity island (cag pai). we used human cdna ... | 2002 | 12411577 |
| responses of endoscopy patients in ladakh, india, to helicobacter pylori whole-cell and cag a antigens. | although helicobacter pylori is a cosmopolitan colonizer of the human stomach, the responses among persons in remote populations from whom h. pylori was cultured have not been studied. we report on studies of 189 persons in the ladakh region of india in whom serum immunoglobulin g responses to h. pylori whole-cell and cag a antigens were measured. h. pylori was isolated from 68 of these patients. an h. pylori whole-cell antigen derived from ladakhi strains outperformed a similar antigen from u.s ... | 2002 | 12414766 |
| grb2 is a key mediator of helicobacter pylori caga protein activities. | caga delivered from helicobacter pylori into gastric epithelial cells undergoes tyrosine phosphorylation and induces host cell morphological changes. here we show that caga can interact with grb2 both in vitro and in vivo, which results in the activation of the ras/mek/erk pathway and leads to cell scattering as well as proliferation. importantly, this ability of caga is independent from the tyrosine phosphorylation, which occurs within the five repeated epiya sequences (py region) of caga. howe ... | 2002 | 12419219 |
| comparative analysis of helicobacter dnas and biliary pathology in patients with and without hepatobiliary cancer. | several helicobacter species have recently been isolated from the bile and hepatobiliary systems of murine species, and are well recognized as a pathogen of the hepatobiliary disorder. this study was planned to investigate whether helicobacter species possess a causative potential for human hepatobiliary disease, especially for hepatobiliary carcinogenesis. bile and hepatobiliary tissue samples from 19 patients with hepatobiliary cancer and 19 patients with benign biliary diseases were subjected ... | 2002 | 12419842 |
| helicobacter pylori releases a factor(s) inhibiting cell cycle progression of human gastric cell lines by affecting cyclin e/cdk2 kinase activity and rb protein phosphorylation through enhanced p27(kip1) protein expression. | helicobacter pylori, the main cause of chronic gastritis, plays a central role in the etiology of peptic ulcer disease and gastric cancer. in vitro studies have shown that h. pylori increases gastric epithelial cell turnover, thus increasing the risk for the development of neoplastic clones. the mechanisms by which h. pylori promotes perturbation of cell proliferation are not yet elucidated. to investigate whether products released by h. pylori in culture media interfere with cell cycle progress ... | 2002 | 12441136 |
| helicobacter pylori caga protein activates serum response element-driven transcription independently of tyrosine phosphorylation. | infection with helicobacter pylori possessing the cag pathogenicity island (pai) is associated with severe gastritis and gastric cancer. caga protein, one of the products of cag pai, is translocated into epithelial cells, where cytoskeletal rearrangements occur as a result of caga tyrosine (tyr) phosphorylation. here we identify a new role for caga protein as an activator of host cell signaling. | 2002 | 12454853 |
| the vacuolating toxin of helicobacter pylori mimicks the cftr-mediated chloride conductance. | cystic fibrosis (cf) is caused by defects of the cf transmembrane conductance regulator (cftr), which acts both as an anion-selective channel and as a regulator of other proteins. the relative contribution of these two functions in cf disease is debated. the toxin vaca forms channels with properties similar to those of the cftr, and we report here that it can insert into the membrane of various cells originating from respiratory epithelia, generating a chloride conductance comparable to that pro ... | 2002 | 12459497 |
| involvement of tnf-alpha changes in human cancer development, prevention and palliative care. | cancer development and ageing are complex sciences. from the study on the process of rodent carcinogenesis, we identified tumor necrosis factor-alpha (tnf-alpha) as an important mediator of cancer development. this paper presents three clinical examples of tnf-alpha up-regulation: by cord factors of mycobacterium tuberculosis, such as trehalose 6-monomycolate, as an activator of protein kinase c and by a cord factor like fraction of microsporum canis obtained in the air inside houses in thailand ... | 2002 | 12470903 |
| correlation between helicobacter pylori infection and il-18 mrna expression in human gastric biopsy specimens. | our data indicate that h. pylori infection is associated with active interleukin-18 production in patients with chronic gastritis. different cell types appear to be involved in this activity and may play a role in the development of immunopathologic damage. | 2002 | 12095959 |
| interleukin 1beta polymorphisms increase risk of hypochlorhydria and atrophic gastritis and reduce risk of duodenal ulcer recurrence in japan. | interleukin-1 beta (il-1beta) polymorphisms are associated with increased risk of gastric cancer in whites. this study aimed to examine effects of these polymorphisms on gastric acid secretion, atrophic gastritis, and risk of peptic ulcer in japan. | 2002 | 12105837 |
| analysis of p53 mutations and helicobacter pylori infection in human and animal models. | p53 gene mutations are believed to play a critical role in the development of gastric carcinoma. we examined the relation between helicobacter pylori infection and p53 gene mutations of the gastric mucosa in human and animal models. | 2002 | 12109656 |
| helicobacter pylori-induced activation of human endothelial cells. | helicobacter pylori infection causes active chronic inflammation with a continuous recruitment of neutrophils to the inflamed gastric mucosa. to evaluate the role of endothelial cells in this process, we have examined adhesion molecule expression and chemokine and cytokine production from human umbilical vein endothelial cells stimulated with well-characterized h. pylori strains as well as purified proteins. our results indicate that endothelial cells actively contribute to neutrophil recruitmen ... | 2002 | 12117971 |
| randomized open trial for comparison of proton pump inhibitors in triple therapy for helicobacter pylori infection in relation to cyp2c19 genotype. | genetic polymorphism of cytochrome p450 (cyp) 2c19 influences the efficacy of helicobacter pylori eradication therapy with a proton pump inhibitor (ppi) and amoxicillin. however, in triple therapy (ppi plus amoxicillin and clarithromycin), little is known about the impact of cyp2c19 polymorphism, or the use of rabeprazole, which is not well metabolized by cyp2c19. the efficacy of three ppi (omeprazole, lansoprazole, and rabeprazole) in a 1-week triple regimen were compared in relation to cyp2c19 ... | 2002 | 12121503 |
| [association between helicobacter pylori caga strain infection and expression of cyclooxygenase 2 in gastric carcinoma]. | to observe the expression of cyclooxygenase 2 (cox-2) in tissues of human gastric cancer and explore the association between helicobacter pylori caga strain infection and the expression of cox-2 so as to provide a theoretical basis for early prevention of gastric cancer. | 2002 | 12126507 |
| cancer prevention and therapy with kiwifruit in chinese folklore medicine: a study of kiwifruit extracts. | kiwi gold fruits were extracted successively with hexane, acetone, methanol and 70% methanol, and further fractionated by silica gel and ods column chromatographies for the assays of various biological activities. five fractions h1, h2 (hexane extract), al, a2 (acetone extract) and m2 (methanol extract) showed selective cytotoxic activity against human oral tumor cell lines, which was more sensitive than human gingival fibroblasts. more hydrophilic fractions [70m3, 70m4, 70m5] of 70% methanol ex ... | 2002 | 12127237 |
| helicobacter pylori may survive ampicillin treatment in the remnant stomach. | helicobacter pylori (h. pylori) is a gram-negative curved rod-like or spiral bacterium that chronically infects the human gastric mucosa, and is a major risk factor for gastritis, gastric and duodenal ulcer and adenocarcinoma of the stomach. after partial gastrectomy, some patients may have persistent h. pylori infection for five years or more. in this study, we detected three bacteria, i.e., klebsiella pneumoniae, enterobacter aerogenes, and escherichia coli, in the gastric juice of patients wi ... | 2002 | 12139018 |
| genome-scale metabolic model of helicobacter pylori 26695. | a genome-scale metabolic model of helicobacter pylori 26695 was constructed from genome sequence annotation, biochemical, and physiological data. this represents an in silico model largely derived from genomic information for an organism for which there is substantially less biochemical information available relative to previously modeled organisms such as escherichia coli. the reconstructed metabolic network contains 388 enzymatic and transport reactions and accounts for 291 open reading frames ... | 2002 | 12142428 |
| new strategies for the prevention and treatment of helicobacter pylori infection. | helicobacter pylori infects the stomach of > 50% of the human population worldwide, with higher prevalence in the developing countries. a strict correlation between h. pylori infection and gastroduodenal diseases has been demonstrated, including gastritis, peptic ulcer and gastric cancer. current therapies against h. pylori consist of an antisecretory plus antibiotics. these therapies are effective in 80 - 90% of the cases; presently, no alternative therapies have been shown to give comparable o ... | 2002 | 12150706 |
| helicobacter pylori virulence genes and host il-1rn and il-1beta genes interplay in favouring the development of peptic ulcer and intestinal metaplasia. | helicobacter pylori infection outcome might depend on genotypic polymorphisms of both the bacterium and the host. we ascertained: (1) the functionality of h. pylori oipa gene; (2) the polymorphism of the hostinterleukin (il-1beta) gene (-31 c/t) and of the il-1rn gene (intron 2 vntr); (3) the association between the above genes and the histological and pathological outcome of h. pylori infection. one hundred and sixty-five h. pylori positive and 137 h. pylori negative subjects (23 gastric adenoc ... | 2002 | 12161099 |
| synchronous primary adenocarcinoma, mucosa-associated lymphoid tissue lymphoma and a stromal tumor in a helicobacter pylori-infected stomach. | we report the case of a 78-year-old man with helicobacter pylori infection and three primary neoplasms of the stomach: adenocarcinoma, mucosa-associated lymphoid tissue (malt) lymphoma and a gastrointestinal stromal cell tumor. helicobacter pylori infection is associated with gastric carcinoma and malt lymphoma, although their simultaneous occurrence is rare. gastrointestinal stromal cell tumors have not been associated with infection to date. this appears to be the first report of the synchrono ... | 2002 | 12167128 |
| expression of cyclooxygenase-2 in primary and remnant gastric carcinoma: comparing it with p53 accumulation, helicobacter pylori infection, and vascular endothelial growth factor expression. | cyclooxygenase-2 (cox-2) expression may contribute to the synthesis of prostanoids, which have been related to carcinogenesis and tumor progression. it is well known that the gastric remnant is at greater risk of the development of gastric cancer than is the whole stomach; incidence rates for gastric cardia adenocarcinoma are rising in the united states and europe. our objective was to determine the involvement of cox-2 in primary and remnant gastric cancer tissues as well as in adjacent noncanc ... | 2002 | 12173384 |
| inhibitory effect of lactobacillus acidophilus on helicobacter pylori in peptic ulcer patients: in vitro study. | lactobacillus acidophilus (l. acidophilus) have been introduced into many fermented dairy products. the presence of l. acidophilus appears to decrease helicobacter pylori (h. pylori) density in the human stomach and could enhance antibiotic therapy for h. pylori eradication. this study was designed to determine the optimal density of l. acidophilus that has a maximum inhibitory effect on h. pylori in peptic ulcer patients. to determine whether l. acidophilus has an inhibitory effect on h. pylori ... | 2002 | 12188456 |
| [expression of pi glutathione s-transferase in intestinal metaplasia and its relationship with helicobacter pylori infection]. | to study the dynamic change of glutathione s- transferase pi (gst-pi) in normal gastric mucosa, gastric mucosa with intestinal metaplasia (im) and gastric cancer and to investigate the relationship between human carcinogen detoxification system and the virulence of helicobacter pylori(h.pylori) in the stage of im. | 2002 | 12194793 |
| pathogenesis of helicobacter pylori infection. | five years after publication of the complete genome sequence of helicobacter pylori, research interest is shifting from the descriptive association of virulence factors with clinical outcome in infected patients to the molecular mechanisms of virulence factor action. this is particularly noticeable for vaca and caga, for both of which detailed understanding of the interaction with host signalling pathways has accumulated over the last year. the role of h. pylori lewis antigens for clinical outco ... | 2002 | 12197905 |
| effect of weissella confusa strain pl9001 on the adherence and growth of helicobacter pylori. | viable and nonviable weissella confusa strain pl9001 inhibited the binding of helicobacter pylori to human gastric-cell line mkn-45 cells by more than 90%. spent culture supernatant of pl9001 rapidly decreased the viability of h. pylori, rupturing cell walls. the results suggest that pl9001 is a probiotic that can reduce the infectivity and persistence of h. pylori. | 2002 | 12200324 |
| dip, the database of interacting proteins: a research tool for studying cellular networks of protein interactions. | the database of interacting proteins (dip: http://dip.doe-mbi.ucla.edu) is a database that documents experimentally determined protein-protein interactions. it provides the scientific community with an integrated set of tools for browsing and extracting information about protein interaction networks. as of september 2001, the dip catalogs approximately 11 000 unique interactions among 5900 proteins from >80 organisms; the vast majority from yeast, helicobacter pylori and human. tools have been d ... | 2002 | 11752321 |
| human plasma is directly bacteriocidal against helicobacter pylori in vitro, potentially explaining the decreased detection of helicobacter pylori during acute upper gi bleeding. | the rapid urease test is the preferred method for detection of h pylori in patients with uncomplicated ulcer disease undergoing egd. however, the sensitivity of this test when performed during upper gi bleeding has been questioned. it has been suggested that false-negative results occur, resulting from buffering effects of blood. the direct effect of blood was evaluated on h pylori growth in ex vivo and in vitro systems. | 2002 | 11756907 |
| effect of cold starvation, acid stress, and nutrients on metabolic activity of helicobacter pylori. | helicobacter pylori can transform, in vivo as well as in vitro, from dividing spiral-shaped forms into nonculturable coccoids, with intermediate forms called u forms. the importance of nonculturable coccoid forms of h. pylori in disease transmission and antibiotic treatment failures is unclear. metabolic activities of actively growing as well as nonculturable h. pylori were investigated by comparing the concentrations of cellular atp and total rna, gene expression, presence of cytoplasmic polyph ... | 2002 | 11772603 |
| helicobacter pylori stimulates pepsinogen secretion from isolated human peptic cells. | different acid and peptic related gastroduodenal diseases are associated with both increased gastric secretion and helicobacter pylori infection. patients with h pylori associated gastritis or duodenal ulcer have increased serum pepsinogen levels which decrease after eradication. the mechanisms of h pylori induced gastric mucosal damage are not completely understood. | 2002 | 11772960 |
| src is the kinase of the helicobacter pylori caga protein in vitro and in vivo. | the gastric pathogen helicobacter pylori uses a type iv secretion system to inject the bacterial caga protein into gastric epithelial cells. within the host cell, caga becomes phosphorylated on tyrosine residues and initiates cytoskeletal rearrangements. we demonstrate here that src-like protein-tyrosine kinases mediate caga phosphorylation in vitro and in vivo. first, the src-specific tyrosine kinase inhibitor pp2 specifically blocks caga phosphorylation and cytoskeletal rearrangements thereby ... | 2002 | 11788577 |
| adams (a disintegrin and metalloproteinase) messenger rna expression in helicobacter pylori-infected, normal, and neoplastic gastric mucosa. | helicobacter pylori is a risk factor for gastric cancer, and bacterial-epithelial interactions may be critical in this association. studies using complementary dna arrays indicated that the adam (a disintegrin and metalloproteinase) genes in gastric epithelial cells are differentially expressed after bacterial-epithelial interactions. reverse-transcription polymerase chain reaction analysis of gastric biopsy specimens from patients with and without h. pylori showed that infection was associated ... | 2002 | 11807715 |
| association analysis of polymorphisms at the interleukin-1 locus in essential hypertension. | infection with microorganisms such as helicobacter pylori and chlamydia pneumoniae has been associated with coronary heart disease (cad) and hypertension (ht). infection increases the release of pro-inflammatory cytokines, thus facilitating interactions that lead to vascular damage and other effects. we hypothesized that genetically determined differences in activity or responsiveness of cytokine(s) might contribute to ht. the interleukin-1 gene (il1) cluster on chromosome 2q14 contains three re ... | 2002 | 11840488 |
| review article: natural history and epidemiology of helicobacter pylori infection. | helicobacter pylori is a common bacterium infecting about half the world's population. it is causally linked with a diverse spectrum of gastrointestinal clinical disorders including peptic ulcer disease, gastric cancer, and gastric malt lymphoma. the principal reservoir is the human stomach, and transmission probably occurs by person-to-person passage. prevalence rates are generally much higher in developing countries compared to developed countries, although there are subgroups within many regi ... | 2002 | 11849122 |
| helicobacter pylori activates the proto-oncogene c-fos through sre transactivation. | epidemiological studies have demonstrated a strong association between helicobacter pylori infection and gastric cancer. however, there have been few detailed studies on the mechanism of cellular proliferation by h. pylori. thus, we examined activation of the proto-oncogene c-fos to elucidate the underlying mechanism of cell proliferation caused by h. pylori. activation of c-fos was evaluated in human gastric cancer cells (tmk1) by northern blot and reporter assays with deletion analysis of the ... | 2002 | 11866445 |
| extracellular ph modulates helicobacter pylori-induced vacuolation and vaca toxin internalization in human gastric epithelial cells. | in this study we investigated whether an acidic extracellular ph may inhibit h. pylori-induced internalization of bacterial virulence factors by gastric epithelium, thus preventing ingestion of potentially dangerous luminal contents and resulting cellular damage. the interaction of h. pylori vaca toxin and ammonia (produced by h. pylori urease) with partly polarized gastric mkn 28 cells in culture was investigated at neutral and moderately acidic ph (6.2, compatible with cell viability) by means ... | 2002 | 11890688 |
| effect of lactoferrin on helicobacter felis induced gastritis. | lactoferrin possesses antibiotic, antiinflammatory, and immune-modulating properties that may be active against the gastritis-, ulcer- and cancer-inducing bacterium helicobacter pylori. in vitro testing of bovine and human lactoferrin by several laboratories has shown significant bacteriostatic and bactericidal activity. subsequent in vivo testing of bovine lactoferrin in animal models of h. pylori infection has shown beneficial effects of this agent. our laboratory has utilized a mouse model th ... | 2002 | 11908634 |
| bacterial secrets of secretion: euroconference on the biology of type iv secretion processes. | type iv secretion systems (tfss) mediate secretion or direct cell-to-cell transfer of virulence factors (proteins or protein-dna complexes) from many gram-negative animal, human and plant pathogens, such as agrobacterium tumefaciens, bartonella tribocorum, bordetella pertussis, brucella suis, helicobacter pylori, legionella pneumophila and rickettsia prowazekii, into eukaryotic cells. bacterial conjugation is also classified as a tfss-like process mediating the spread of broad-host-range plasmid ... | 2002 | 11918819 |
| immunoproteomics of helicobacter pylori infection and relation to gastric disease. | the gram negative bacterium helicobacter pylori is a human pathogen which infects the gastric mucosa and causes an inflammatory process leading to gastritis, ulceration and cancer. a systematic, proteome based approach was chosen to detect candidate antigens of h. pylori for diagnosis, therapy and vaccine development and to investigate potential associations between specific immune responses and manifestations of disease. sera from patients with active h. pylori infection (n = 24), a control gro ... | 2002 | 11921447 |
| critical role of an endogenous gastric peroxidase in controlling oxidative damage in h. pylori-mediated and nonmediated gastric ulcer. | the objective of the present study is to delineate the mechanism of oxidative damage in human gastric ulcerated mucosa despite the presence of some antioxidant enzymes. we report for the first time the critical role of an endogenous peroxidase, a major h(2)o(2) metabolizing enzyme, in controlling oxidative damage in gastric mucosa. human gastric mucosa contains a highly active peroxidase in addition to the myeloperoxidase contributed by neutrophil. in both non-helicobacter pylori (h. pylori)- an ... | 2002 | 11937299 |
| removal of mucus for ultrastructural observation of the surface of human gastric epithelium using pronase. | helicobacter pylori adhering to the human gastric epithelium causes gastric diseases such as ulcer, carcinoma and lymphoma. it is thus important to observe in detail both the surface of the epithelial cells and the h. pylori that adhered to it for the elucidation of h. pylori-induced diseases by scanning electron microscopy (sem). since the thick mucus layer blocks the observation of the cell surface and the bacteria, it is generally eliminated during the processing for sem by roughly mechanical ... | 2002 | 11966871 |
| differential glycosylation of mhc class ii molecules on gastric epithelial cells: implications in local immune responses. | class ii major histocompatibility complex (mhc) expression is a hallmark of antigen presenting cells (apc). human gastric epithelial cells (gec) express class ii mhc and this expression increases during infection with helicobacter pylori as does the number of cd4 t cells found adjacent or in between epithelial cells. these observations suggested that human gec act as apcs. to characterize and compare class ii mhc complexes with those present in conventional apc, immunoprecipitated class ii mhc f ... | 2002 | 11975982 |