Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| behaviour of the pathogen surrogates listeria innocua and clostridium sporogenes during production of parsley in fields fertilized with contaminated amendments. | the survival and transfer of listeria innocua and clostridium sporogenes, used as surrogates of the food borne pathogens listeria monocytogenes and clostridium botulinum, were quantitatively assessed under field conditions. in the soil, spores of c. sporogenes declined by less than 0.7 log cycles within 16 months and were detected on parsley leaves throughout the experiment. in contrast, l. innocua in the soil declined by 7 log cycles in 90 days and was detected on leaves in low numbers (>0.04 m ... | 2005 | 16332327 |
| effect of immersion solutions containing enterocin as-48 on listeria monocytogenes in vegetable foods. | the effect of immersion solutions containing enterocin as-48 alone or in combination with chemical preservatives on survival and proliferation of listeria monocytogenes cect 4032 inoculated on fresh alfalfa sprouts, soybean sprouts, and green asparagus was tested. immersion treatments (5 min at room temperature) with as-48 solutions (25 microg/ml) reduced listeria counts of artificially contaminated alfalfa and soybean sprouts by approximately 2.0 to 2.4 log cfu/g compared to a control immersion ... | 2005 | 16332751 |
| triphenylmethane reductase from citrobacter sp. strain kctc 18061p: purification, characterization, gene cloning, and overexpression of a functional protein in escherichia coli. | we purified to homogeneity an enzyme from citrobacter sp. strain kctc 18061p capable of decolorizing triphenylmethane dyes. the native form of the enzyme was identified as a homodimer with a subunit molecular mass of about 31 kda. it catalyzes the nadh-dependent reduction of triphenylmethane dyes, with remarkable substrate specificity related to dye structure. maximal enzyme activity occurred at ph 9.0 and 60 degrees c. the enzymatic reaction product of the triphenylmethane dye crystal violet wa ... | 2005 | 16332773 |
| exogenous isoleucine and fatty acid shortening ensure the high content of anteiso-c15:0 fatty acid required for low-temperature growth of listeria monocytogenes. | previous studies have demonstrated that the branched-chain fatty acid anteiso-c15:0 plays a critical role in the growth of listeria monocytogenes at low temperatures by ensuring sufficient membrane fluidity. studies utilizing a chemically defined minimal medium revealed that the anteiso fatty acid precursor isoleucine largely determined the fatty acid profile and fatty acid response of the organism to lowered growth temperature. when isoleucine was sufficient, the fatty acid profile was very uni ... | 2005 | 16332779 |
| relatedness of listeria monocytogenes isolates recovered from selected ready-to-eat foods and listeriosis patients in the united states. | pulsed-field gel electrophoresis and serotyping were performed for 544 isolates of listeria monocytogenes, including 502 isolates recovered from contaminated samples from 31,705 retail ready-to-eat (rte) food products and 42 isolates recovered from human cases of listeriosis. the isolates were from maryland (294 isolates) and california (250 isolates) and were collected in 2000 and 2001. the isolates were placed into 16 asci pulsogroups (level of relatedness within each group, > or =66%), 139 as ... | 2005 | 16332793 |
| listeria monocytogenes perr mutants display a small-colony phenotype, increased sensitivity to hydrogen peroxide, and significantly reduced murine virulence. | deletion of perr in listeria monocytogenes results in a small-colony phenotype (deltaperrsm) that is slow growing and exhibits increased sensitivity to h2o2. at a relatively high frequency, large-colony variants (deltaperrlg) arise, which are more resistant to h2o2 than the wild-type and ultimately dominate the culture. transcriptional analysis revealed that the kat gene (catalase) is up-regulated in both types of mutants and that the highest level is apparent in deltaperrsm mutants, demonstrati ... | 2005 | 16332818 |
| contingency locus in ctsr of listeria monocytogenes scott a: a strategy for occurrence of abundant piezotolerant isolates within clonal populations. | in a recent study we demonstrated that a high-hydrostatic-pressure-tolerant isolate of listeria monocytogenes lacks a codon in the class 3 heat shock regulator gene ctsr. this mutation in the region that encodes four consecutive glycines was directly responsible for the observed piezotolerance, increased stress resistance, and reduced virulence. the aim of the present study was to determine whether mutations in ctsr are frequently associated with piezotolerance in l. monocytogenes. wild-type cul ... | 2005 | 16332826 |
| select listeria monocytogenes subtypes commonly found in foods carry distinct nonsense mutations in inla, leading to expression of truncated and secreted internalin a, and are associated with a reduced invasion phenotype for human intestinal epithelial cells. | the surface protein internalin a (inla) contributes to the invasion of human intestinal epithelial cells by listeria monocytogenes. screening of l. monocytogenes strains isolated from human clinical cases (n=46), foods (n=118), and healthy animals (n=58) in the united states revealed mutations in inla leading to premature stop codons (pmscs) in l. monocytogenes ribotypes dup-1052a and dup-16635a (pmsc mutation type 1), dup-1025a and dup-1031a (pmsc mutation type 2), and dup-1046b and dup-1062a ( ... | 2005 | 16332872 |
| a novel real-time pcr for listeria monocytogenes that monitors analytical performance via an internal amplification control. | we describe a novel quantitative real-time (q)-pcr assay for listeria monocytogenes based on the coamplification of a target hly gene fragment and an internal amplification control (iac). the iac is a chimeric double-stranded dna containing a fragment of the rapeseed bnaccg8 gene flanked by the hly-specific target sequences. this iac is detected using a second taqman probe labeled with a different fluorophore, enabling the simultaneous monitoring of the hly and iac signals. the hly-iac assay had ... | 2005 | 16332910 |
| [evaluation of the antimicrobial action of honey against staphylococcus aureus, staphylococcus epidermidis, pseudomonas aeruginosa, escherichia coli, salmonella enteritidis, listeria monocytogenes and aspergillus niger. evaluation of its microbiological charge]. | the evaluation of the microbiological charge present in costa rican samples as the evaluation of its antimicrobial activity over different microorganisms, including those associated to wound infections, will allow to emit criteria referred to its use in therapeutic treatments, specially as alternative therapy for cases involving antibiotic resistant bacteria. the microbiological charge of 25 honey samples, acquired in costa rican markets was evaluated through several indicators including total p ... | 2005 | 16335227 |
| simultaneous deficiency of both mura and p60 proteins generates a rough phenotype in listeria monocytogenes. | we examined eight spontaneously occurring rough mutants of listeria monocytogenes for their ability to express two previously reported autolysins, p60 and mura. all mutants lack mura expression and show strongly reduced levels of extracellular p60. one rough strain harbors a variant of the p60 protein with a partially truncated catalytic domain. in seven cases there were shifts in the localization of p60 to the membrane fraction. mutations within the seca2 gene, encoding an auxiliary protein sec ... | 2005 | 16321943 |
| immunosuppression related to collagen-vascular disease or its treatment. | collagen-vascular diseases are associated with immune dysregulation and inflammation, leading to tissue destruction or compromise. immunosuppression is more commonly associated with the drugs used to treat these disorders than with the diseases themselves. the newest agents being used to treat collagen-vascular diseases are the tumor necrosis factor (tnf)-alpha inhibitors. u.s. food and drug administration-approved tnf-alpha inhibitors have differing effects on the immune system, reflecting thei ... | 2005 | 16322600 |
| the activity of pathway-selective estrogen receptor ligands in experimental septic shock. | estrogen receptors (er) are widely expressed in multiple genital and nongenital tissues. upon engagement of these receptors, multiple genes are affected in target tissues via estrogen response elements. nonsteroidal pathway-selective er ligands have recently been identified that inhibit nf-kappab transcriptional activity and are devoid of conventional estrogenic activities on genital tissues. these pathway-selective ligands are potent anti-inflammatory agents in vivo and may prove to be of thera ... | 2005 | 16317384 |
| [misleading presentation of rhombencephalitis due to listeria monocytogenes]. | rhombencephalitis due to listeria monocytogenes may present in a variety of forms. | 2005 | 16317592 |
| listeriolysin o, a cytolysin derived from listeria monocytogenes, inhibits generation of ovalbumin-specific th2 immune response by skewing maturation of antigen-specific t cells into th1 cells. | listeriolysin o (llo), a cholesterol-dependent cytolysin derived from listeria monocytogenes, is a potent inducer of interleukin (il)-12, il-18 and interferon (ifn)-gamma. we have shown that llo facilitates development of t cells mediating protective immunity against l. monocytogenes through the induction of ifn-gamma production at an early stage. based on this finding, it is postulated that llo inhibits differentiation of th2 cells and the th2 immune response. by using a murine model of ovalbum ... | 2005 | 16232213 |
| evaluation of chromogenic media for the detection of listeria species in food. | the aim of this study was to evaluate the performance of chromogenic agars, agar listeria according to ottaviani and agosti (aloa) and rapid l. mono agar, compared with oxford agar for the enumeration and detection of listeria species in food. | 2005 | 16313406 |
| environmental factors influencing the relationship between optical density and cell count for listeria monocytogenes. | the effect of temperature (2-30 degrees c), ph (4.8-7.4) and water activity (0.946-0.995) on the relationship between optical density (od) at 600 nm and the plate count (cfu ml(-1)) was investigated for listeria monocytogenes. | 2005 | 16313423 |
| anti-bacterial activity of lactobacillus plantarum strain sk1 against listeria monocytogenes is due to lactic acid production. | the aim of this research was to investigate the potential of lactobacillus plantarum strain sk1 for use as a biological control agent against listeria monocytogenes and determine its mechanism of anti-listerial activity. | 2005 | 16313424 |
| membrane tumor necrosis factor confers partial protection to listeria infection. | tumor necrosis factor (tnf) plays a critical role in the host response to the intracellular pathogen listeria monocytogenes (lm). tnf exists in soluble and membrane-bound forms and exhibits both unique and overlapping activities. we examined the role of membrane tnf in the absence of secreted tnf for host resistance in knockin mice in which the endogenous tnf was replaced by a regulated, noncleavable allele (mem-tnf). macrophages expressing mem-tnf produced nitric oxide and displayed normal bact ... | 2005 | 16314479 |
| identification of unusual bacterial glycosylation by tandem mass spectrometry analyses of intact proteins. | the characterization of protein glycosylation can be a complex and time-consuming procedure, especially for prokaryote o-linked glycoproteins, which often comprise unusual oligosaccharide structures with no known glycosylation motif. in this report, we describe a "top-down" approach that provides information on the extent of glycosylation, the molecular masses, and the structure of oligosaccharide residues on bacterial flagella, important structural proteins involved in the motility of pathogeni ... | 2005 | 16316188 |
| stochastically modeling listeria monocytogenes growth in farm tank milk. | this article presents a listeria monocytogenes growth model in milk at the farm bulk tank stage. the main objective was to judge the feasibility and value to risk assessors of introducing a complex model, including a complete thermal model, within a microbial quantitative risk assessment scheme. predictive microbiology models are used under varying temperature conditions to predict bacterial growth. input distributions are estimated based on data in the literature, when it is available. if not, ... | 2005 | 16297223 |
| loading history determines the velocity of actin-network growth. | directional polymerization of actin filaments in branched networks is one of the most powerful force-generating systems in eukaryotic cells. growth of densely cross-linked actin networks drives cell crawling, intracellular transport of vesicles and organelles, and movement of intracellular pathogens such as listeria monocytogenes. using a modified atomic force microscope (afm), we obtained force-velocity (fv) measurements of growing actin networks in vitro until network elongation ceased at the ... | 2005 | 16299496 |
| neurological complications in kidney transplant recipients. | neurological complications are frequent in renal transplant recipients and may largely contribute to morbidity and mortality. the postransplant neurological complications may be categorized into five areas: 1) immunosuppressive medications, 2) stroke, 3) peripheral neuropathies, 4) infection, and 5) malignancies. a number of complications are directly caused by the neurotoxicity of immunosuppressive agents. calcineurin-inhibitors may cause mild symptoms, such as tremors and paresthesia, or sever ... | 2005 | 16299677 |
| use of oligonucleotide array for identification of six foodborne pathogens and pseudomonas aeruginosa grown on selective media. | identification of presumptive foodborne pathogens grown on selective media may take one to several days and requires a different battery of biochemical tests for each microorganism. a molecular identification method was developed in which universal primers were used to amplify the 16s to 23s rdna intergenic spacer of target microorganisms, and pcr products were hybridized to a panel of species-specific oligonucleotides that were immobilized on a nylon membrane. the seven target microorganisms we ... | 2005 | 16300063 |
| quantifying the robustness of a broth-based model for predicting listeria monocytogenes growth in meat and poultry products. | given the importance of listeria monocytogenes as a risk factor in meat and poultry products, there is a need to evaluate the relative robustness of predictive growth models applied to meat products. the u.s. department of agriculture-agricultural research service pathogen modeling program is a tool widely used by the food industry to estimate pathogen growth, survival, and inactivation in food. however, the robustness of the pathogen modeling program broth-based l. monocytogenes growth model in ... | 2005 | 16300067 |
| listeria monocytogenes inhibition by whey protein films and coatings incorporating lysozyme. | the effects of whey protein isolate (wpi) films and coatings incorporating lysozyme (lz) on the inhibition of listeria monocytogenes both in and on microbial media, as well as on cold-smoked salmon, were studied. the antimicrobial effects of lz were examined using various growth media by turbidity and plate counting tests. disc-covering and disc-surface-spreading tests were also used to evaluate the effects of wpi films incorporating lz. smoked salmon was used as a model food to test the antimic ... | 2005 | 16300068 |
| application of predictive models to estimate listeria monocytogenes growth on frankfurters treated with organic acid salts. | organic acid salts including sodium lactate, sodium diacetate, potassium benzoate, potassium sorbate, and their combinations were assessed as potential inhibitors of listeria monocytogenes growth on frankfurters. predictive models for l. monocytogenes growth on frankfurters treated with these salts were compared to select a proper l. monocytogenes growth curve model under these conditions. sigmoidal equations, including logistic and gompertz equations, are widely used to describe bacterial growt ... | 2005 | 16300069 |
| power ultrasound treatment of listeria monocytogenes in apple cider. | inactivation experiments with listeria monocytogenes 10403s, an ultrasound-resistant strain, were conducted at sublethal (20, 30, and 40 degrees c) and lethal (50, 55, and 60 degrees c) temperatures in saline solution (ph 7.0), acidified saline solution (ph 3.4), and apple cider (ph 3.4) with and without application of ultrasound (20 khz, 457 mw.ml(-l)). the survival of recoverable l. monocytogenes 10403s in apple cider was evaluated, and the effects of temperature, ultrasound, ph, and food matr ... | 2005 | 16300070 |
| starter cultures and high-pressure processing to improve the hygiene and safety of slightly fermented sausages. | the effectiveness of selected starter cultures and high hydrostatic pressure after ripening was evaluated to improve the safety and quality of slightly fermented sausages. inhibition of common foodborne pathogens, spoilage bacteria, and biogenic amine content was studied. random amplification of polymorphic dna and plasmid profiles were used to monitor the competitiveness of the starter cultures during fermentation and ripening. lactobacillus sakei ctc6626 and staphylococcus xylosus ctc6013 domi ... | 2005 | 16300071 |
| efficacy of cetylpyridinium chloride against listeria monocytogenes and its influence on color and texture of cooked roast beef. | sliced (cut) and exterior (intact) surfaces of restructured cooked roast beef were inoculated with listeria monocytogenes, treated with cetylpyridinium chloride (cpc; immersion in 500 ml of 1% solution for 1 min), individually vacuum packaged, and stored for 42 days at 0 or 4 degrees c. noninoculated samples were similarly treated, packaged, and stored to determine effects on quality (color and firmness) and on naturally occurring bacterial populations, including aerobic plate counts and lactic ... | 2005 | 16300072 |
| listeria monocytogenes survival in refrigerator dill pickles. | listeria monocytogenes can survive and grow in refrigerated foods with ph values of approximately 4.0 to 5.0 and salt concentrations of 3 to 4%. home-fermented refrigerator dill pickles fit this description. contamination of this product with l. monocytogenes could cause serious problems because these items are not heated prior to consumption. l. monocytogenes survival and growth patterns were investigated in refrigerator dill pickles at 1.3, 3.8, and 7.6% salt concentrations. pickling cucumbers ... | 2005 | 16300073 |
| consequences of the development of nisin-resistant listeria monocytogenes in fermented dairy products. | wild listeria isolates representing serovars found in artisanal cheeses commercialized in asturias (northern spain) were assessed for their susceptibility to several bacteriocins. pediocin pa-1 was the most active bacteriocin followed by enterocin as-48, nisin, and plantaricin c. however, some listeria monocytogenes and listeria innocua strains were already highly resistant to pa-1. among the wild l. monocytogenes populations, the frequency of development of nisin resistance ranged from 10(-6) u ... | 2005 | 16300077 |
| thermal tolerance of acid-adapted and unadapted salmonella, escherichia coli o157:h7, and listeria monocytogenes in cantaloupe juice and watermelon juice. | a study was performed to determine d values of acid-adapted and unadapted cells of salmonella, escherichia coli o157:h7, and listeria monocytogenes in cantaloupe juice and watermelon juice. | 2005 | 16305669 |
| [experimental study on impact of salmon milt dna on t-cellular immunity]. | the impact of a biologically active food supplement containing salmon milt dna on cellular immunity was experimentally studied. it was shown that the dna had a dose-dependent stimulating effect on lymphocyte transformation, stimulated the dth response and protected from experimental listeriosis infection. | 2005 | 16308934 |
| retinoic acid amplifies the host immune response to lps through increased t lymphocytes number and lps binding protein expression. | vitamin a deficiency is associated with increased susceptibility to infection but the effects of vitamin a supplementation on host response to pathogens are controversial. this study investigated the mechanisms by which all-trans retinoic acid (atra) modulates the host immune response in an experimental model of vitamin a supplementation before and after challenge with lps in rats. we show here that a supplementation with five daily injections of 10mg/kg atra increased the number of t lymphocyte ... | 2005 | 16309824 |
| enzyme-electropolymer-based amperometric biosensors: an innovative platform for time-temperature integrators. | a novel exogenous time-temperature integrator (tti) based on an amperometric glucose oxidase biosensor is presented. the tti consists of the enzyme entrapped within an electrochemically generated poly(o-phenylenediamine) (popd) thin film deposited on the interior wall of a platinum (pt) or a platinized stainless steel (pt-ss) capsule. after thermal treatment, the tti is mounted in a continuous flow system and connected to a potentiostat for amperometric detection of residual enzyme activity. a m ... | 2005 | 16277377 |
| detection and characterization of tet(m) in tetracycline-resistant listeria strains from human and food-processing origins in belgium and france. | in the present study, three listeria monocytogenes strains and one listeria innocua strain out of a collection of 241 listeria isolates from human and food-processing sources were found to display resistance to tetracycline (tc) due to the presence of the tet(m) gene. through sequence analysis, it was shown that tet(m) genes in two of the isolates belong to sequence homology group (shg) ii, a group comprising chromosomally encoded tet(m) genes previously found in staphylococcus aureus and in lac ... | 2005 | 16278428 |
| surveillance of listeria infections in europe. | in addition to the economic consequences and threats associated with outbreaks, listeriosis remains of great public health concern, as it has one of the highest case fatality rates of all the foodborne infections (20%-30%), and has common source epidemic potential. changes in the way food is produced, distributed and stored have created the potential for diffuse and widespread outbreaks involving many countries. in 2002, a survey was carried out to assess the need for and the feasibility of a eu ... | 2005 | 16282642 |
| cellular lipid fatty acid pattern heterogeneity between reference and recent food isolates of listeria monocytogenes as a response to cold stress. | cells of four reference strains (scott a, lo 28, cnl 895807 and atcc 19115) and of five recent food isolates (a00m011, a00m018, a00m087, a00m092 and a00m123) of listeria monocytogenes were grown until late exponential phase in brain heart broth at two different temperatures (37 degrees c and 4 degrees c). our results show that significant differences exist between the cellular lipid fatty acid profile of reference and recent food isolates. like the reference strains, and in keeping with previous ... | 2005 | 16284926 |
| [focal peritonitis as a form of clinical presentation of listeriosis]. | infections by germs of listeria genus can occur sporadically or in epidemic outbreaks and have different clinical presentations. abdominal origin is very unusual among them, especially in no immunocompromised patients or without concurrent liver illness. we present a young healthy man who is lacking in underlying diseases, suffering from focal peritonitis caused by listeria monocytogenes. the patient had to be operated on, requiring immediate colectomy and evacuation of a retroperitoneal abscess ... | 2005 | 16288580 |
| effectiveness of antimicrobial food packaging materials. | antimicrobial additives have been used successfully for many years as direct food additives. the literature provides evidence that some of these additives may be effective as indirect food additives incorporated into food packaging materials. antimicrobial food packaging is directed toward the reduction of surface contamination of processed, prepared foods such as sliced meats and frankfurter sausages (hot dogs). the use of such packaging materials is not meant to be a substitute for good sanita ... | 2005 | 16227182 |
| response of escherichia coli o157:h7, listeria monocytogenes, salmonella typhimurium, and staphylococcus aureus to the thermal stress occurring in model manufactures of grana padano cheese. | the purpose of this research was to investigate the effect of temperature in the technology of production of grana cheese against escherichia coli o157:h7, listeria monocytogenes, salmonella typhimurium, and staphylococcus aureus. according to the technology of production, the cheese curds are cooked at 55 degrees c and then cooled at room temperature (25 degrees c). a curd-cooling model was developed to estimate the temperature variation across the curd during cooling, and the thermal stress wa ... | 2005 | 16230687 |
| [spontaneous bacterial peritonitis by listeria monocytogenes in a patient with liver cirrhosis]. | 2005 | 16266228 | |
| listeria monocytogenes meningitis following imatinib mesylate-induced monocytopenia in a patient with chronic myeloid leukemia. | 2005 | 16267747 | |
| application of measurements of transepithelial electrical resistance of intestinal epithelial cell monolayers to evaluate probiotic activity. | among five potentially probiotic lactobacilli investigated, lactobacillus plantarum mf1298 and lactobacillus salivarius dc5 showed the highest increase in the transepithelial electrical resistance (ter) of polarized monolayers of caco-2 cells, and this increase was shown to be dose dependent. furthermore, preincubation with mf1298 attenuated a decrease in ter induced by listeria monocytogenes. | 2005 | 16269795 |
| occurrence of listeria spp. in effluents of french urban wastewater treatment plants. | listeria spp. were found in most treated waters (84.4%) and raw sludge (89.2%) of six french urban wastewater treatment plants and one composting facility, examined monthly over a 1-year period. most strains belonged to listeria monocytogenes, serotypes 4b/4e being predominant. sludge composting and liming reduced or prevented listeria contamination. | 2005 | 16269804 |
| factors influencing the ability of listeria monocytogenes to pass through a membrane filter by active infiltration. | listeria monocytogenes infiltrated the reticulate structure of a membrane filter and passed through a filter with pore sizes of 0.45 microm and 0.2 microm in 6 to 24 h and 5 to 6 days, respectively. flagellar motility and expansive pressure generated by the growing bacterial population were indicated as the driving forces of infiltration. | 2005 | 16269806 |
| thymus-dependent memory phenotype cd8 t cells in naive b6.h-2kb-/-db-/- animals mediate an antigen-specific response against listeria monocytogenes. | b6.h-2kb-/-db-/- (dko) mice have greatly reduced numbers of mature cd8alphabeta t cells in their periphery. however, these non-class ia-selected cd8alphabeta t cells are able to mediate immune responses to a number of pathogens. approximately 60% of the cd8alphabeta t cells in the spleen and peripheral lymph nodes of naive dko mice display a memory (cd44high) phenotype. to investigate the origins of these non-class ia-selected cd8alphabetacd44high cells, we traced the phenotype of recent thymic ... | 2005 | 16272298 |
| regulation of expression of type i signal peptidases in listeria monocytogenes. | the role of type i signal peptidases (spases i) is to remove the signal peptides of preproteins exported by the general secretory pathway. the genome of listeria monocytogenes contains a locus encoding three contiguous spases i (denoted sipx, sipy and sipz). the authors recently showed that sipx and sipz perform distinct functions in protein secretion and bacterial pathogenicity. here, the regulation of sip gene expression in broth and in infected eukaryotic cells was studied. the results show t ... | 2005 | 16272398 |
| the food safety management system. | a comprehensive food safety strategy involves establishing risk management goals, food safety objectives and, for production systems, performance objectives and performance criteria. the working instructions for each step of the process should be validated for their effect before integration within a specific haccp plan. the importance of realistic inactivation models to predict the hygienic equivalence of food processing operations is discussed. | 2005 | 16244936 |
| molecular characterization of listeria monocytogenes strains associated with outbreaks of listeriosis in humans and ruminants and food products by serotyping and automated ribotyping. | 2005 | 16244967 | |
| antimicrobial effect of thai spices against listeria monocytogenes and salmonella typhimurium dt104. | the objective of this study was to determine the potential antimicrobial activity of extracts and essential oils of spices from thailand against foodborne pathogenic bacteria. the antimicrobial efficacy of ginger (zingiber officinale), fingerroot (boesenbergia pandurata), and turmeric (curcuma longa) was evaluated against five strains of listeria monocytogenes and four strains of salmonella enterica ssp. enterica serovar typhimurium dt104. antimicrobial activity was investigated in microbiologic ... | 2005 | 16245707 |
| evaluation of small-scale hot-water postpackaging pasteurization treatments for destruction of listeria monocytogenes on ready-to-eat beef snack sticks and natural-casing wieners. | this study was conducted to evaluate small-scale hot-water postpackaging pasteurization (ppp) as a postlethality (post-cooking) treatment for listeria monocytogenes on ready-to-eat beef snack sticks and natural-casing wieners. using a commercially available plastic packaging film specifically designed for ppp applications and 2.8 liters of boiling water (100 degrees c) in a sauce pan on a hot plate, an average reduction in l. monocytogenes numbers of > or = 2 log units was obtained using heating ... | 2005 | 16245708 |
| antilisterial activity of a carnobacterium piscicola isolated from brazilian smoked fish (surubim [pseudoplatystoma sp.]) and its activity against a persistent strain of listeria monocytogenes isolated from surubim. | data on the prevalence and growth of listeria monocytogenes in lightly preserved fish products from subtropical and tropical regions are very scarce. our research describes l. monocytogenes that was detected in 5% of the packages of cold-smoked surubim, a native brazilian freshwater fish that we analyzed, and shows that the strains isolated were of the same random amplified polymorphic dna subtype as the strains that were isolated from the same factory 4 years earlier. a bacteriocinogenic strain ... | 2005 | 16245709 |
| stability of enterocin as-48 in fruit and vegetable juices. | enterocin as-48 is a candidate bacteriocin for food biopreservation. before addressing application of as-48 to vegetable-based foods, the interaction between as-48 and vegetable food components and the stability of as-48 were studied. enterocin as-48 had variable interactions with fruit and vegetable juices, with complete, partial, or negligible loss of activity. for some juices, loss of activity was ameliorated by increasing the bacteriocin concentration, diluting the juice, or applying a heat ... | 2005 | 16245711 |
| efficacy of uv light for the reduction of listeria monocytogenes in goat's milk. | certain types of goat's cheeses are produced using unpasteurized milk, which increases the food safety concerns for these types of products. popularity and consumption of goat's milk products have increased, and the niche market includes gourmet goat's cheeses. the u.s. code of federal regulations and the pasteurized milk ordinance both address the possibility for processing alternatives to heat treatment, and the use of uv light treatment may be a viable alternative that still ensures the safet ... | 2005 | 16245732 |
| [effect of high hydrostatic pressure on microbial physiological characteristics]. | physiological characterizations of listeria monocytogenes nctc 11994 and escherichia coli atcc 80739 have deeply changed by high hydrostatic pressure. the results showed that counts of both microbial strains decreased 7 log cfu at 400mpa, 10 min. pressure treatments also resulted in change of intracellular ph value, lowed membrane potential, have internal potassium filtered out, and decreased atp concentration. | 2005 | 16245862 |
| identification of substrates of the listeria monocytogenes sortases a and b by a non-gel proteomic analysis. | sortases are enzymes that anchor surface proteins to the cell wall of gram-positive bacteria by cleaving a sorting motif located in the c-terminus of the protein substrate. the best-characterized motif is lpxtg, which is cleaved between the t and g residues. in this study, a non-gel proteomic approach was used to identify surface proteins recognized by the two sortases of listeria monocytogenes, srta and srtb. material containing peptidoglycan and strongly associated proteins was purified from s ... | 2005 | 16247833 |
| listeria-based vaccines for cancer treatment. | listeria monocytogenes is a facultative intracellular bacterium that enters professional antigen-presenting cells by active phagocytosis. as a live bacterium, it induces antigen-presenting cell maturation and strong innate immunity which may assist in the immune response to poorly immunogenic antigens, such as tumor-associated antigens. listeria produces virulence factors that allow it to escape from the phagolysosome and colonize the cytosol of the host cell. it is thus a potent vaccine vector ... | 2005 | 16248280 |
| comparison of genomic structures in the serovar 1/2a listeria monocytogenes isolated from meats and listeriosis patients in japan. | foodborne disease by listeria monocytogenes, serovar 1/2a has recently been reported in many countries. although contamination by this bacteria is also known to be gradually spreading among the marketed foods of japan, there is little information on relation between listeriosis and food contamination. in the present study, the characteristics of the genomic structures of serovar 1/2a were compared among the isolates from marketed meats and listeriosis patients. several isolates from meats purcha ... | 2005 | 16249623 |
| analysis of the molecular evolution of listeria monocytogenes isolated from japanese meats and environment. | food contaminated by listeria monocytogenes is a problem on a worldwide level because it is a serious food-borne pathogen. although 3 evolutionary divisions have been reported for l. monocytogenes, the evolution of japanese isolates has not yet been clarified. thus, in order to determine the lineage of these japanese isolates, we classified and conducted phylogenetic analysis of 407 bp (position 1116-1522) of the iap gene derived from 88 isolates from japanese listeriosis patients, foods and env ... | 2005 | 16249630 |
| adhesion controls bacterial actin polymerization-based movement. | as part of its infectious life cycle, the bacterial pathogen listeria monocytogenes propels itself through the host-cell cytoplasm by triggering the polymerization of host-cell actin near the bacterial surface, harnessing the activity of several cytoskeletal proteins used during actin-based cell crawling. to distinguish among several classes of biophysical models of actin-based bacterial movement, we used a high-throughput tracking technique to record the movement of many individual bacteria dur ... | 2005 | 16251274 |
| roles of group iia phospholipase a2 and complement in killing of bacteria by acute phase serum. | the complement system is regarded as an important component of the innate defence system against invading bacteria. however, synergistic actions between the complement and the other components of innate immunity are incompletely known. human group iia phospholipase a(2) (hgiia pla(2)) is an effective antibacterial enzyme in serum of patients with severe bacterial infections. our aim was to investigate the significance of complement and hgiia pla(2) in acute phase serum. serum samples were collec ... | 2005 | 16253130 |
| arp2/3 complex-deficient mouse fibroblasts are viable and have normal leading-edge actin structure and function. | rna interference silencing of up to 90% of arp3 protein expression, a major subunit of the arp2/3 complex, proportionately decreases the intracellular motility of listeria monocytogenes and actin nucleation activity ascribable to the arp2/3 complex in mouse embryonic fibroblasts. however, the arp2/3-deficient cells exhibit unimpaired lamellipodial actin network structure, translational locomotion, spreading, actin assembly, and ruffling responses. in addition, arp3-silenced cells expressing neur ... | 2005 | 16254049 |
| real-time polymerase chain reaction for detecting bacterial dna directly from blood of neonates being evaluated for sepsis. | speed is of the essence when evaluating an infant with symptoms consistent with sepsis. because of the high morbidity and mortality associated with neonatal sepsis, infants receive multiple, broad-spectrum antibiotics before receiving finalized blood culture results. incorporating an additional, reliable, yet rapid assay to detect bacteria directly from blood would facilitate timely diagnosis and appropriate care. to this end, we designed a real-time polymerase chain reaction (pcr) assay targeti ... | 2005 | 16258155 |
| [do we need pathogen-free livestock for food safety?]. | the reduction of the entry of pathogens in the food chain is an important premise in improving the safety of food of animal origin. since food animals are the main reservoir for pathogens, the prevalence of pathogens in livestock is of great importance. unfortunately, classical measures to improve animal health can not exclude the presence of the main food-borne zoonotic agents (salmonella, campylobacter, listeria monocytogenes, verotoxinogene escherichia coli, toxoplasma gondii) in "clinically ... | 2005 | 16218183 |
| antibacterial and antioxidant activities of essential oils isolated from thymbra capitata l. (cav.) andoriganum vulgare l. | antilisterial activities of thymbra capitata and origanum vulgare essential oils were tested against 41 strains of listeria monocytogenes. the oil of t. capitata was mainly constituted by one component, carvacrol (79%), whereas for o. vulgare three components constituted 70% of the oil, namely, thymol (33%), gamma-terpinene (26%), and p-cymene (11%). t. capitata essential oil had a significantly higher antilisterial activity in comparison to o. vulgare oil and chloramphenicol. no significant dif ... | 2005 | 16218659 |
| outbreak of clinical listeriosis in sheep: evaluation from possible contamination routes from feed to raw produce and humans. | we report the results of clinical and microbiological investigations on listeria monocytogenes infections in a flock of 55 sheep and describe the implications for the safety of the raw milk and raw-milk cheeses produced in the on-farm dairy. the outbreak was caused by feeding grass silage, which was contaminated with 5 log10 cfu l. monocytogenes/g. clinically, although having been fed from the same batch of silage, abortive (nine ewes), encephalitic (one ewe) and septicaemic (four ewes) forms of ... | 2005 | 16219091 |
| conductivity and ph dual detection of growth profile of healthy and stressed listeria monocytogenes. | in this study, growth of listeria monocytogenes in a low conductivity growth medium (lcgm) was simultaneously monitored by conductivity and ph measurements. detection times obtained from the conductivity and ph growth curves were inversely related to the initial concentration of l. monocytogenes in the medium. linear responses were found by plotting detection times obtained from both conductivity and ph growth curves as a function of initial cell concentration in the range of 10(2) to 10(7) cfu/ ... | 2005 | 16167332 |
| functional consequences of genome evolution in listeria monocytogenes: the lmo0423 and lmo0422 genes encode sigmac and lstr, a lineage ii-specific heat shock system. | listeria monocytogenes strains belonging to phylogenetic lineage ii (serotypes 1/2a, 1/2c, and 3a) carry a lineage-specific genome segment encoding a putative sigma subunit of rna polymerase (lmo0423, herein referred to as sigc), a gene of unknown function (lmo0422) similar to the padr family of regulators, and a gene that is similar to the roda-ftsw family of cell wall morphology genes (lmo0421). to understand the function of this set of genes, their expression patterns and the effects of null ... | 2005 | 16237008 |
| distinct regulation of h2-m3-restricted memory t cell responses in lymph node and spleen. | cd8 t cell populations restricted by h2-m3 mhc class ib molecules expand rapidly during primary listeria monocytogenes infection but only minimally upon reinfection. in contrast, cd8 t cells restricted by mhc class ia molecules undergo more delayed expansion during primary infection but rapid and robust expansion following reinfection. in this study we demonstrate that primary h2-m3-restricted cd8 t cell responses are unaffected by the frequency of naive mhc class ia-restricted t cells during l. ... | 2005 | 16237094 |
| osteopontin is not required for the development of th1 responses and viral immunity. | osteopontin (opn) has been defined as a key cytokine promoting the release of il-12 and hence inducing the development of protective cell-mediated immunity to viruses and intracellular pathogens. to further characterize the role of opn in antiviral immunity, opn-deficient (opn-/-) mice were analyzed after infection with influenza virus and vaccinia virus. surprisingly, we found that viral clearance, lung inflammation, and recruitment of effector t cells to the lung were unaffected in opn-/- mice ... | 2005 | 16237095 |
| the importance of toll-like receptor 2 polymorphisms in severe infections. | toll-like receptor 2 (tlr2) is a member of the tlr family, which plays a central role in the innate immune response to a wide variety of microorganisms. animal studies have shown that tlr2-knockout mice are more susceptible to septicemia due to staphylococcus aureus and listeria monocytogenes, meningitis due to streptococcus pneumoniae, and infection with mycobacterium tuberculosis, suggesting that functional tlr2 polymorphisms may impair host response to a certain spectrum of microbial pathogen ... | 2005 | 16237639 |
| growth rate and growth probability of listeria monocytogenes in dairy, meat and seafood products in suboptimal conditions. | to evaluate the performances of models predicting the growth rate or the growth probability of listeria monocytogenes in food. | 2005 | 16238733 |
| the autolytic phenotype of the bacillus cereus group. | to determine the autolytic phenotype of five species in the bacillus cereus group. | 2005 | 16238737 |
| an investigation of the thermal inactivation of staphylococcus aureus and the potential for increased thermotolerance as a result of chilled storage. | the aims of this study were; (i) to provide thermal inactivation data for staphylococcus aureus; (ii) to examine the kinetics, including decimal reduction times (d-value) and rate constants (k), that describe the thermal inactivation of staph. aureus and to compare two different methods of calculating d-values and (iii) to determine whether or not chilled storage would toughen these microorganisms resulting in increased thermotolerance. | 2005 | 16238754 |
| gene silencing and overexpression of porcine peptidoglycan recognition protein long isoforms: involvement in beta-defensin-1 expression. | peptidoglycan recognition proteins (pgrps) are a group of newly identified proteins with emerging functions in mammalian innate immunity. here we report the identification and characterization of two long isoforms of porcine pgrp. their complete cdna sequences encode predicted peptides of 252 and 598 residues and are named ppgrp-l1 and ppgrp-l2, respectively. these porcine isoforms share identical pgrp domains at their c terminus, which are highly conserved with human and mouse orthologs. ppgrp- ... | 2005 | 16239507 |
| a prfa transposon mutant of listeria monocytogenes f2365, a serotype 4b strain, is able to survive in the gastrointestinal tract but does not cause systemic infection of the spleens and livers of intragastrically inoculated mice. | prfa is a member of the crp/fnr family of global regulatory genes in listeria monocytogenes that has been shown previously to regulate several key virulence determinants both in vitro and in parenterally inoculated laboratory rodents. however, the role of prfa in the ability of l. monocytogenes to cause infection via the gastrointestinal (gi) tract has not been clearly established. in this study, we used a prfa transposon mutant of l. monocytogenes f2365, a serotype 4b strain, to assess the role ... | 2005 | 16239554 |
| typing of food-borne listeria monocytogenes by polymerase chain reaction-restriction enzyme analysis and amplified fragment length polymorphism. | the applicability of polymerase chain reaction-restriction enzyme analysis (pcr-rea) and amplified fragment length polymorphism (aflp) for typing of food borne listeria monocytogenes strains was tested. a panel of 43 l. monocytogenes strains isolated from food, mostly serovars 1/2b or 1/2a, were analysed by the optimized pcr-rea oriented to inla and inlb genes and by aflp. by pcr-rea, five types of profiles were obtained. by aflp, the strains were separated into 11 types and 18 subtypes forming ... | 2005 | 16240700 |
| identification and characterization of di- and tripeptide transporter dtpt of listeria monocytogenes egd-e. | listeria monocytogenes is a gram-positive intracellular pathogen responsible for opportunistic infections in humans and animals. here we identified and characterized the dtpt gene (lmo0555) of l. monocytogenes egd-e, encoding the di- and tripeptide transporter, and assessed its role in growth under various environmental conditions as well as in the virulence of l. monocytogenes. uptake of the dipeptide pro-[14c]ala was mediated by the dtpt transporter and was abrogated in a deltadtpt isogenic de ... | 2005 | 16204487 |
| long-term survival of pathogenic and sanitation indicator bacteria in experimental biowaste composts. | for economic, agricultural, and environmental reasons, composting is frequently used for organic waste recycling. one approach to limiting the potential risk from bacterial food-borne illnesses is to ensure that soil amendments and organic fertilizers are disinfected. however, more knowledge concerning the microbiological safety of composted substrates other than sludge and manure is necessary. experimental in-vessel biowaste composts were used to study the survival of seeded listeria monocytoge ... | 2005 | 16204488 |
| microarray analysis of a two-component regulatory system involved in acid resistance and proteolytic activity in lactobacillus acidophilus. | two-component regulatory systems are one primary mechanism for environmental sensing and signal transduction. annotation of the complete genome sequence of the probiotic bacterium lactobacillus acidophilus ncfm revealed nine two-component regulatory systems. in this study, the histidine protein kinase of a two-component regulatory system (lba1524hpk-lba1525rr), similar to the acid-related system lisrk from listeria monocytogenes (p. d. cotter et al., j. bacteriol. 181:6840-6843, 1999), was inser ... | 2005 | 16204490 |
| genetic diversity of listeria monocytogenes strains from a high-prevalence dairy farm. | listeria monocytogenes is a significant food-borne human and veterinary pathogen. contaminated silage commonly leads to disease in livestock, but the pervasive nature of the bacterium can make it difficult to identify the source of infection. an investigation of bovine listeriosis that occurred on a pacific northwest dairy farm ("farm a") revealed that the clinical strain was closely related to fecal strains from asymptomatic cows, and that farm environment was heavily contaminated with a divers ... | 2005 | 16204502 |
| investigation of specific substitutions in virulence genes characterizing phenotypic groups of low-virulence field strains of listeria monocytogenes. | several models have shown that virulence varies from one strain of listeria monocytogenes to another, but little is known about the cause of low virulence. twenty-six field l. monocytogenes strains were shown to be of low virulence in a plaque-forming assay and in a subcutaneous inoculation test in mice. using the results of cell infection assays and phospholipase activities, the low-virulence strains were assigned to one of four groups by cluster analysis and then virulence-related genes were s ... | 2005 | 16204519 |
| rsa 2004: combined basic research satellite symposium-mechanisms of alcohol-mediated organ and tissue damage: inflammation and immunity and alcohol and mitochondrial metabolism: at the crossroads of life and death session one: alcohol, cellular and organ damage. | this article summarizes content proceedings of a satellite meeting held at the 2004 research society on alcoholism annual scientific meeting in vancouver, canada. the aim of the satellite conference was to facilitate the interaction of scientists investigating the mechanisms of alcohol-mediated organ or tissue damage, and enable the discussion and sharing of new ideas and concepts that may be common in each of the organs or tissues affected by chronic ethanol consumption. the original planned pr ... | 2005 | 16205374 |
| sigmab contributes to listeria monocytogenes invasion by controlling expression of inla and inlb. | the ability of listeria monocytogenes to invade non-phagocytic cells is important for development of a systemic listeriosis infection. the authors previously reported that a l. monocytogenes delta sigb strain is defective in invasion into human intestinal epithelial cells, in part, due to decreased expression of a major invasion gene, inla. to characterize additional invasion mechanisms under the control of sigmab, mutants were generated carrying combinations of in-frame deletions in inla, inlb ... | 2005 | 16207905 |
| multi-virulence-locus sequence typing clarifies epidemiology of recent listeriosis outbreaks in the united states. | multi-virulence-locus sequence typing (mvlst) was used to analyze isolates from two major listeriosis outbreaks in the united states in 1998 and 2002 that were due to consumption of contaminated hot dogs and turkey deli meat, respectively. mvlst demonstrated high epidemiological relevance and indicated that the two outbreaks were the result of one epidemic. | 2005 | 16208000 |
| paradoxical anti-inflammatory actions of tnf-alpha: inhibition of il-12 and il-23 via tnf receptor 1 in macrophages and dendritic cells. | il-12 and tnf-alpha are central proinflammatory cytokines produced by macrophages and dendritic cells. disregulation of tnf-alpha is associated with sepsis and autoimmune diseases such as rheumatoid arthritis. however, new evidence suggests an anti-inflammatory role for tnf-alpha. tnf-alpha-treated murine macrophages produced less il-12p70 and il-23, after stimulation with ifn-gamma and lps. frequency of il-12p40-producing macrophages correspondingly decreased as measured by intracellular cytoki ... | 2005 | 16210605 |
| attenuation of th1 response in decoy receptor 3 transgenic mice. | the soluble decoy receptor 3 (dcr3) is a member of the tnfr superfamily. because dcr3 is up-regulated in tumor tissues and is detectable in the sera of cancer patients, it is regarded as an immunosuppressor to down-regulate immune responses. to understand the function of dcr3 in vivo, we generated transgenic mice overexpressing dcr3 systemically. in comparison with hnt-tcr (hnt) transgenic mice, up-regulation of il-4 and il-10 and down-regulation of ifn-gamma, il-12, and tnf-alpha were observed ... | 2005 | 16210617 |
| purification of bovine milk lactoperoxidase and investigation of antibacterial properties at different thiocyanate mediated. | bovine lactoperoxidase (lpo) was purified with amberlite cg 50 h+ resin, cm sephadex c-50 ion-exchange chromatography, and sephadex g-100 gel filtration chromatography from skim milk. the activity of lactoperoxidase was measured by using 2.2-azino-bis (3-ethylbenzthiazoline-6 sulfonic acid) diammonium salt (abts) as a choromogenic substrate at ph 6.0. purification degree for the purified enzyme was controlled with sds-page and rz value (a412/a280). rz value for the purified lpo was 0.8. km value ... | 2005 | 16212035 |
| response regulator degu of listeria monocytogenes regulates the expression of flagella-specific genes. | an isogenic mutant of listeria monocytogenes egd with a deletion of the response regulator gene degu showed a lack of motility due to the absence of flagella. in the present study, we used two-dimensional gel electrophoresis, mass-spectrometry and microarray analyses to identify the listerial genes that depend on degu for expression. we found that the two l. monocytogenes operons encoding flagella-specific genes and the monocistronically transcribed flaa gene are positively regulated by degu at ... | 2005 | 16213668 |
| correlation of probiotic lactobacillus salivarius growth phase with its cell wall-associated proteome. | lactobacillus salivarius subsp. salivarius ucc118 is a probiotic bacterium that was originally isolated from human intestinal tissues and was subsequently shown in a pilot study to alleviate symptoms associated with mild-moderate crohn's disease. strain ucc118 can adhere to animal and human intestinal tissue, and to both healthy and inflamed ulcerative colitis mucosa, irrespective of location in the gut. in this study, an enzymatic technique has been combined with proteomic analysis to correlate ... | 2005 | 16214296 |
| [lymphocytis meningitis: listeria monocytogenes is a potential risk in a immunocompetent child]. | meningoencephalitis due to listeria monocytogenes is a rare and serious form of brainstem infection in childhood. observation: we report the case of a 7 year-old girl presenting lymphocytic meningitis with a high crp level. parenteral antibiotics combining ceftriaxone and vancomycine led initially to clinical improvement. ten days later, secondary brainstem inflammation with hydrocephalus appeared and led to the detection of l. monocytogenes during external ventricular bypass. conclusion: this o ... | 2005 | 16185855 |
| effectiveness of steam pasteurization in controlling microbiological hazards of cull cow carcasses in a commercial plant. | the purpose of the study, carried out in a beef processing plant, was to evaluate the effectiveness of a new prototype for steam pasteurization treatment in controlling microbiological hazards. samples were taken by swabbing randomly selected sites before and after pasteurization and again after chilling to obtain total aerobic counts (tac), total coliform counts (tcc), and generic escherichia coli counts (ecc) on petrifilm plates and to determine the prevalence of salmonella spp., listeria mono ... | 2005 | 16187550 |
| bacteriophage p100 for control of listeria monocytogenes in foods: genome sequence, bioinformatic analyses, oral toxicity study, and application. | listeria monocytogenes is an opportunistic foodborne pathogen responsible for listeriosis, a frequently fatal infection. this investigation represents a comprehensive approach to characterize and evaluate the broad host range, strictly virulent phage p100, which can infect and kill a majority of listeria monocytogenes strains. first, the complete nucleotide sequence (131,384 basepairs) of the genome of p100 was determined, predicted to encode 174 gene products and 18 trnas. bioinformatic analyse ... | 2005 | 16188359 |
| potential antimicrobial effects of human lactoferrin against oral infection with listeria monocytogenes in mice. | listeria monocytogenes is a food-borne pathogen that causes serious listeriosis in humans. antimicrobial effects of human lactoferrin (hlf) against l. monocytogenes have been clearly demonstrated in in vitro studies. however, in vivo studies have not been reported yet. this study investigated whether the oral administration of hlf could inhibit oral infection of listeria in balb/c mice. the mics for several strains of l. monocytogenes were determined, and the most sensitive strain was used for t ... | 2005 | 16192436 |
| neuropathological findings in 9 cases of listeria monocytogenes brain stem encephalitis. | brain stem encephalitis is a particular manifestation of infection with the bacterium listeria monocytogenes. here, we present the neuropathological findings in 9 such cases. in the brain stem, the inflammatory infiltrates were located predominantly within nuclei and tracts of cranial nerves innervating the oropharynx. these findings support the hypothesis that the food-borne bacterium listeria monocytogenes invades the brain stem along cranial nerves. | 2005 | 16196384 |
| symmetry-breaking motility. | locomotion of bacteria by actin polymerization and in vitro motion of spherical beads coated with a protein catalyzing polymerization are examples of active motility. starting from a simple model of forces locally normal to the surface of a bead, we construct a phenomenological equation for its motion. the singularities at a continuous transition between moving and stationary beads are shown to be related to the symmetries of its shape. universal features of the phase behavior are calculated ana ... | 2005 | 16197183 |
| genomic fingerprinting of bacteriocin-producer strains of staphylococcus aureus. | among 363 strains of staphylococcus aureus, 21 were shown to produce bacteriocins (bac), antimicrobial peptides with potential biotechnological applications. this collection includes strains which are either isolated from food, patients and healthy cattle, or are involved in subclinical bovine mastitis. from these 21 strains, 17 were shown to carry closely-related 8.0-kb bac plasmids encoding bacteriocins either identical to or similar to aureocin a70, a bacteriocin able to inhibit strains of li ... | 2005 | 16171981 |
| high pressure processing for food safety. | food preservation using high pressure is a promising technique in food industry as it offers numerous opportunities for developing new foods with extended shelf-life, high nutritional value and excellent organoleptic characteristics. high pressure is an alternative to thermal processing. the resistance of microorganisms to pressure varies considerably depending on the pressure range applied, temperature and treatment duration, and type of microorganism. generally, gram-positive bacteria are more ... | 2005 | 16175246 |