Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| measurement of branched-chain ketoacids in plasma by high-performance liquid chromatography. | branched-chain ketoacids were isolated from plasma or serum samples by acidification, passage through a cationic exchange resin, ether extraction, and extraction of the ether layer with phosphate buffer. the recovery of 2-[1-14c]ketoisocaproate taken through these procedures averaged 95 +/- 3%. branched-chain ketoacids were measured by high-performance liquid chromatography using a single mobile phase (sodium phosphate:acetonitrile). in normal human subjects, mean +/- sd fasting levels of 2-keto ... | 1987 | 3674376 |
| sample preparation for gas chromatography with electron capture detection: determination of total and free thyroxin in serum. | relatively clean gas chromatography with electron capture detection (gc-ecd) chromatograms are obtained for both total and free thyroxin (t4) in serum by improving sample preparation. this is based on establishing a sequence of steps that cumulatively overcome two classes of interference: those present in the initial sample and those introduced by the procedure. the main source for the latter contaminants is the derivatization step, a problem that was largely overcome by employing hplc after thi ... | 1987 | 3674391 |
| effects of growth hormone-releasing hormone and corticotropin-releasing hormone on the release of thyrotropin-releasing hormone from the rat hypothalamus in vitro. | effects of growth hormone-releasing hormone (grh) and corticotropin-releasing hormone (crh) on the release of immunoreactive thyrotropin-releasing hormone (ir-trh) from the rat hypothalamus in vitro were studied. the rat hypothalamus was incubated in medium 199 with 1.0 mg/ml of bacitracin (ph 7.4) for 20 min. the amount of ir-trh release into the medium was measured by radioimmunoassay. the ir-trh release from the rat hypothalamus was inhibited significantly in a dose-related manner with the ad ... | 1987 | 3131150 |
| on the mechanism of antithrombotic action of flavonoids. | flavonols (quercetin and rutin) and flavanes (cyanidol and meciadonol) were studied for their effect on non-enzymatic lipid peroxidation, lipoxygenase and cyclo-oxygenase activities, binding to albumin and platelet membranes. these biochemical properties of four flavonoids were compared with respect to their antithrombotic action in vivo and their efficacy at influencing the platelet-endothelium interaction in vitro. all four flavonoids inhibited the ascorbate-stimulated formation of malondialde ... | 1987 | 3101704 |
| epinephrine, glucose, and lactate infusion in exercising adrenodemedullated rats. | the purpose of this study was to determine the metabolic function of the marked increase in plasma epinephrine which occurs in fasted rats during treadmill exercise. fasted adrenodemedullated (adm) and sham-operated (sham) rats were run on a rodent treadmill (21 m/min, 15% grade) for 30 min or until exhaustion. adm rats were infused with saline, epinephrine, glucose, or lactate during the exercise bouts. adm saline-infused rats showed markedly reduced endurance, hypoglycemia, elevated plasma ins ... | 1987 | 3298192 |
| coagulation defects of aflatoxin intoxicated rabbits. | twelve new zealand white rabbits were intoxicated with aflatoxin b1. most rabbits developed a coagulation defect near the time of death. immediately prior to death there were significant decreases in factors v, vii, and viii coagulant activities and fibrinogen concentration without a change in plasma fibrin(ogen) degradation product concentration, platelet number, and detectable plasma fibrin monomers. microscopic evidence of disseminated intravascular coagulation was present in one rabbit with ... | 1987 | 3103313 |
| characterization of self-transmissible plasmids determining lactose fermentation and multiple antibiotic resistance in clinical strains of klebsiella pneumoniae. | the lactose fermentation (lac+) and antibiotic resistance (r+) phenotypes were conjugally transferred from klebsiella pneumoniae strains (k166, k182, k186, k218, and k220) to salmonella typhi, s. typhimurium, shigella flexneri, and vibrio cholerae. the genes for lactose fermentation and antibiotic resistance were located on the plasmids. further analysis of plasmid dna from these isolates indicated the presence of multiple plasmids (mr ranged less than 2.7 to 70 x 10(6)). the lac+r+ plasmids p16 ... | 1987 | 3107002 |
| osteonectin content in human osteogenesis imperfecta bone shows a range similar to that of two bovine models of oi. | samples of human osteogenesis imperfecta (oi) bone were analyzed for osteonectin content by sds gel electrophoresis and immunodetection on western blots. the oi bone osteonectin content varied from normal to severely depressed. previously, we showed that two clinically identical but genetically unrelated bovine models of oi were differentiated biochemically by their bone osteonectin content: one oi model had normal bone osteonectin while the other was severely depressed in this parameter. the da ... | 1987 | 3107778 |
| in vivo studies on intracellular ph, focal flow, and vessel diameter in the cat cerebral cortex: effects of altered co2 and electrical stimulation. | the time course of changes in cortical tissue ph (phi) and blood flow during cortical seizures in halothane-anesthetized cats was examined. the clearance of the molecular form of umbelliferone (um) was used to estimate focal cortical blood flow (cbfu), whereas the ratio of the molecular to the ionic form of the molecule was used to concurrently calculate the local phi. resting phi and flow in normocarbic animals was 7.116 +/- 0.008 and 46 +/- 8 ml/100 g/min, respectively. respiratory induced alt ... | 1987 | 3108271 |
| pulmonary clearance of pseudomonas aeruginosa in neutropenic mice. effects of systemic immunization. | systemic immunization with killed pseudomonas improves pulmonary clearance of these bacteria in normal mice. to test the effects of active systemic immunization on early pulmonary clearance of pseudomonas in neutropenic mice, balb/c mice were intraperitoneally immunized with 10(8) pseudomonas or injected with saline twice weekly for 2 wk. ten days after the last immunization, immunized and control mice received tail vein injections with either nitrogen mustard or saline daily for 3 days. two day ... | 1987 | 3109290 |
| inner-city survey for tuberculosis: evaluation of diagnostic methods. | a total of 1,271 persons living in a socially and economically depressed, inner-city area of vancouver, british columbia, voluntarily attended a tuberculosis case-finding campaign. chest x-ray, on-the-spot specimen of sputum, and tuberculin skin test were offered at the time of the first attendance. all 3 diagnostic methods were found to be well accepted, with 93% of the participants having an x-ray, over 95% producing a sputum specimen, and almost 95% having a tuberculin test (a quarter of thes ... | 1987 | 3109292 |
| effects in rats of dietary protein inadequacy on lactose production, milk volume and components of the lactose synthetase complex (ec 2.4.1.22). | lactose synthetase (ls) is a complex of alpha-lactalbumin and n-acetylglucosamine galactosyl transferase (gt). ls is the rate-limiting enzyme in lactose biosynthesis. lactose is the main osmotic component of milk and may thereby affect milk volume. dietary protein inadequacy reduces milk volume and lactose content. the study investigates the role of ls activity in these phenomena. lactating rats were fed adequate and inadequate amounts of protein of high (milk protein) and low (cereal protein) q ... | 1987 | 3109312 |
| purification and characterization of dihydrofolate reductase from soybean seedlings. | dihydrofolate reductase (dhfr; ec 1.5.1.3) was purified to homogeneity from soybean seedlings by affinity chromatography on methotrexate-aminohexyl sepharose, gel filtration on ultrogel aca-54, and blue sepharose chromatography. sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the enzyme gave a single protein band corresponding to a molecular weight of 22,000. the enzyme is not a 140,000 da heteropolymer as reported by others. amino acid sequence-specific antibodies to intact human d ... | 1987 | 3109322 |
| studies on the rate-determining factor in testosterone hydroxylation by rat liver microsomal cytochrome p-450: evidence against cytochrome p-450 isozyme:isozyme interactions. | the aim of the present study was to examine a recent proposal that inhibitory isozyme:isozyme interactions explain why membrane-bound isozymes of rat liver microsomal cytochrome p-450 exert only a fraction of the catalytic activity they express when purified and reconstituted with saturating amounts of nadph-cytochrome p-450 reductase and optimal amounts of dilauroylphosphatidylcholine. the different pathways of testosterone hydroxylation catalyzed by cytochromes p-450a (7 alpha-hydroxylation), ... | 1987 | 3109324 |
| recombinant human tissue-type plasminogen activator therapy in acute thromboembolic stroke. | systemic fibrinolytic therapy for acute stroke is no longer recommended because of resulting systemic fibrinolysis and the risk of intracerebral hemorrhage. human tissue-type plasminogen activator (tpa) is a native enzyme that converts plasminogen to plasmin with subsequent clot lysis. the affinity for plasminogen is increased several-fold when the substrate is bound to fibrin. at appropriate dosage, "clot-specific" thrombolysis may be achieved at the surface of the thrombus without creating sys ... | 1987 | 3112328 |
| histological changes following transplantation of developing teeth to more advanced functional positions. | 1987 | 3113409 | |
| efficacy of a polyvalent protein-polysaccharide dysentery oral vaccine. | 1987 | 3115716 | |
| investigation of a health care worker with symptomatic human immunodeficiency virus infection: an epidemiologic approach. | 1987 | 3116462 | |
| the inhibition by valproic acid of the mitochondrial oxidation of monocarboxylic and omega-hydroxymonocarboxylic acids: possible implications for the metabolism of gamma-aminobutyric acid. | the interactions of 1-5 mm valproic acid with the hepatic fatty acid oxidation are here described. valproic acid was not substrate for hepatic peroxisomal fatty acid oxidation. its activation outside the mitochondrial matrix compartment was poor when compared to that of octanoic acid, a fatty acid containing the same number of carbones. valproic acid did not inhibit the fatty acyl-coa oxidase nor the cyanide-insensitive acyl-coa oxidation. valproic acid inhibited the mitochondrial oxidations of ... | 1987 | 3117781 |
| a new immunomodulating compound (as-101) with potential therapeutic application. | there has been interest in the potential of synthetic compounds to modify immune responses by imitation of cytokine action. direct administration of interleukin 2 (il-2) in conjunction with adoptive transfer of lymphokine activated killer cells has been used in the treatment of cancer, but there are toxic effects resulting from the high doses of il-2 required. we have developed a new synthetic compound, ammonium tri-chloro(dioxoethylene-o,o'-)tellurate (as-101), which has immunomodulating proper ... | 1987 | 3118216 |
| gonadotropin regulation of tissue-type and urokinase-type plasminogen activators in rat granulosa and theca-interstitial cells during the periovulatory period. | plasminogen activators (pas) are believed to be involved in ovulation. because both tissue-type plasminogen activator (tpa) and urokinase-type plasminogen activator (upa) are secreted by cultured rat granulosa cells, we have examined the activities of these proteins in ovarian homogenates as well as granulosa and theca-interstitial (ti) cells during gonadotropin-induced ovulation. immature rats were injected with 20 iu pregnant mare serum gonadotropin (pmsg) to initiate follicle development, fol ... | 1987 | 3121412 |
| evaluation of the 11co2 positron emission tomographic method for measuring brain ph. i. ph changes measured in states of altered pco2. | the 11co2 method for measuring local brain ph with positron emission tomography (pet) has been experimentally evaluated, testing the adequacy of the kinetic model and the ability of the method to measure changes in brain ph. plasma and tissue time/activity curves measured during and following continuous inhalation of 11co2 were fit with a kinetic model that includes effects of tissue ph, blood flow, and fixation of co2 into compounds other than dissolved gas and bicarbonate ions. for each of ten ... | 1987 | 3121647 |
| lactate dehydrogenase and malate dehydrogenase of sertoli cells in rats. | lactate-dehydrogenase (ldh) and malate-dehydrogenase (mdh) activities and isozyme patterns of maturing sertoli cells were investigated in cultured cells obtained from 2-5-week-old rats. both enzymes, and more strikingly ldh, increased between 2 and 3 weeks of age. ldh isozyme complement included all five isozymes and, in the more mature rats, had a shift toward the isoforms rich in subunit. these data are consistent with an increasing production of lactate/pyruvate and with a finer regulation of ... | 1987 | 3122678 |
| polymicrobial septicemia due to shigella flexneri and pseudomonas aeruginosa: first report. | an invasion of the bloodstream by members of the genus shigella is extremely rare. when it does occur, the etiologic agent is usually a single organism. a report of the first case of shigella bacteremia that was complicated by pseudomonas aeruginosa sepsis is reported. | 1987 | 3123706 |
| effects of cadmium on human platelet reactions. | human platelets incubated with cd2+ took up the cation slowly, and the uptake was speeded up by ionophore a23187. the capacity of human platelets to accumulate cd2+ was large, equivalent to 10 nmol cd2+ per mg protein. the effects of cd2+ on protein phosphorylation and serotonin release of human platelets were studied. washed platelets incubated with cd2+ showed a general increase in protein phosphorylation concurrent with a slow release of serotonin. in the presence of ionophore a23187, however ... | 1987 | 3125819 |
| effects of marine mammal parasites on human health. | 1987 | 3294653 | |
| molecular cloning of invasion plasmid antigen (ipa) genes from shigella flexneri: analysis of ipa gene products and genetic mapping. | tn5-tagged invasion plasmid dna (pwr110) from shigella flexneri serotype 5 (strain m90t) was cloned into the expression vector lambda gt11. recombinant phage (lambda gt11sfl) expressing pwr110-encoded polypeptide antigens were identified by using rabbit antisera directed against s. flexneri m90t invasion plasmid antigens. antigens encoded by lambda gt11sfl recombinant phage were characterized by reacting affinity-purified antibodies, eluted from nitrocellulose-bound plaques of lambda gt11sfl rec ... | 1987 | 3294797 |
| an aminoacyl-trna synthetase complex in escherichia coli. | aminoacyl-trna synthetases from several strains of escherichia coli are shown to elute as a high-molecular-weight complex on 6% agarose columns (bio-gel a-5m). in contrast, very little synthetase activity was observed in such complexes on sephadex g-200 columns, suggesting that these enzymes may interact with or are dissociated during chromatography on dextran. the size of the complex observed on bio-gel a-5m was influenced by the method of cell breakage and the salt concentrations present in bu ... | 1987 | 3294804 |
| an accessory protein identical to mouse egasyn is complexed with rat microsomal beta-glucuronidase and is identical to rat esterase-3. | we report biochemical, immunological, and genetic studies which demonstrate that an accessory protein with the essential features of mouse egasyn is complexed with and stabilizes a portion of beta-glucuronidase in microsomes of rat liver. the accessory protein exists as a complex with beta-glucuronidase since it coprecipitates with beta-glucuronidase after treatment of extracts with a specific beta-glucuronidase antibody. the two proteins are associated by noncovalent bonds since they are easily ... | 1987 | 3294829 |
| bacterial invasion of fibroblasts induces interferon production. | although bacteria and/or bacterial products have been shown to induce interferon (ifn) production in cells of the immune system, the ability of non-lymphoid cells to produce ifn in response to bacterial products and/or bacterial invasion is currently unknown. in this study we examined murine and human fibroblasts for their ability to produce ifn in response to challenge with the invasive bacteria shigella flexneri and enteroinvasive escherichia coli. both human and murine primary cell cultures p ... | 1987 | 3295042 |
| toxic megacolon complicating shigellosis. | 1987 | 3295219 | |
| [dynamics of bacterial polysensitization in acute dysentery in children depending on the species of the causative agent]. | studies on polysensitization, evaluated by the neutrophil damage index, have been made with the use of dysenterin and the allergens of hemolytic staphylococci, enterococci and escherichia coli. the studies have revealed that both specific and nonspecific sensitization to hemolytic staphylococci and enterococci is higher in dysentery caused by shigella flexneri than in dysentery caused by s. sonnei and has the tendency to increase in the course of the disease. | 1987 | 3296580 |
| [gardnerella vaginalis in nonspecific vaginitis]. | 1987 | 3296818 | |
| partial transformation of mouse fibroblastic and epithelial cell lines with the v-myc oncogene. | to investigate the role of the myc gene in mammalian cell transformation, plasmid constructs containing the v-myc oncogene and a co-selectable g418 resistance marker were introduced into both mouse fibroblasts (nih-3t3) and bladder epithelial cells (bbn3 and bbn7). after transfection or microinjection of dna, no transformed foci could be detected on confluent monolayers but, when the cells were cultured under conditions in which individual cells were allowed to grow and form colonies, morphologi ... | 1987 | 3023119 |
| virulence of iron transport mutants of shigella flexneri and utilization of host iron compounds. | mutants of shigella flexneri defective in aerobactin-mediated iron transport were assayed for virulence in several model systems. a tn5 insertion mutant was invasive in hela cells, lethal in the chicken embryo, and produced keratoconjunctivitis in the guinea pig, indicating little or no loss of ability to invade and multiply intracellularly. although the mutant failed to grow in low-iron medium in vitro, growth equivalent to that of the wild type was observed in hela cell lysates. thus, there ap ... | 1987 | 3028962 |
| porous hydroxyapatite as a bone graft substitute in diaphyseal defects: a histometric study. | porous hydroxyapatite (ip200), formed by conversion of the poritidae porites exoskeleton, has pores averaging 230 microns and pore interconnections averaging 190 microns in diameter. in the distal radial diaphyses of 14 dogs, bilateral 7.5 x 20 mm cortical windows were created and fitted with 5 x 7.5 x 20 mm blocks of ip200 implants and iliac autografts. both implanted and contralateral grafted radius specimens were retrieved at 3, 6, 12, 24, and 48 months. unstained undecalcified sections were ... | 1987 | 3029358 |
| on the fidelity of dna replication: herpes dna polymerase and its associated exonuclease. | procaryotic dna polymerases contain an associated 3'----5' exonuclease activity which provides a proofreading function and contributes substantially to replication fidelity. dna polymerases of the eucaryotic herpes-type viruses contain similar associated exonuclease activities. we have investigated the fidelity of polymerases purified from wild type herpes simplex virus, as well as from mutator and antimutator strains. on synthetic templates, the herpes enzymes show greater relative exonuclease ... | 1987 | 3029700 |
| a cellular protein binds to a conserved sequence in the adenovirus type 2 enhancer. | a sensitive gel retention assay has been utilized to detect proteins from uninfected hela nuclei which interact with the adenovirus type 2 enhancer. this assay has been employed to monitor fractionation of nuclear extracts. three enhancer binding factors were resolved by chromatography on deae-sepharose and one of the factors was further purified by chromatography on heparin-sepharose. dnase protection experiments have shown that the heparin-sepharose fraction contains a factor which binds predo ... | 1987 | 3031610 |
| integration and expression of provirus in human cells transformed by avian sarcoma virus. | previously, human diploid fibroblasts from some donors infected in vitro by avian sarcoma virus (asv) were transformed and found, by electron microscopy, to produce small numbers of virus particles that were infectious by bioassay; also, a line of human osteosarcoma cells infected with asv developed additional characteristics of transformation and released a small number of infectious virus particles. in this study the complete proviral sequence was shown to be integrated in the genome of these ... | 1987 | 3033382 |
| effects of inhibitors of the cytoplasmic structures and functions on the early phase of infection of cultured cells with simian virus 40. | to obtain information about cytoplasmic structures and functions involving the entry of simian virus 40 virions into cells, we examined whether the inhibitors that affect the functions and/or structure of lysosomes, cell membrane, and cytoskeletons inhibit expression of nuclear t antigen in the sv40-inoculated rat 3y1 and monkey cv-1 cells. chloroquine, methylamine, and butylamine did not inhibit t-antigen expression, suggesting that lysosomal acidification is not required for establishment of i ... | 1987 | 3033894 |
| the effect of cycloleucine on sfv a7(74) infection in mice. | cycloleucine (cl), a non-metabolizable amino acid analogue, was found to reduce thymus and spleen weights in semliki forest virus (sfv) strain a7(74) infected and control mice. the maximum effects were seen when three daily doses of cl were given to mice 24 h after an sfv a7(74) infection. in these mice thymus atrophy led to abolition of thymus dependent immune responses and changes in the pathological features of the viral infection--the most striking feature being prevention of demyelination. ... | 1987 | 3034321 |
| chronic food restriction and the circadian rhythms of pituitary-adrenal hormones, growth hormone and thyroid-stimulating hormone. | adult male sprague-dawley rats were subjected to food restriction so that they ate 65% of food ingested by control rats. while control rats had free access to food over the 24-hour period, food-restricted rats were provided with food daily at 10 a.m. the experimental period lasted for 34 days. on day 35, rats from both experimental groups were killed at 08.00, 11.00, 14.00, 24.00 and 02.00 h. food restriction modified the circadian rhythms of acth and corticosterone. in addition, total circulati ... | 1987 | 3035995 |
| chronic food restriction and the circadian rhythms of pituitary-adrenal hormones, growth hormone and thyroid-stimulating hormone. | adult male sprague-dawley rats were subjected to food restriction so that they ate 65% of food ingested by control rats. while control rats had free access to food over the 24-hour period, food-restricted rats were provided with food daily at 10 a.m. the experimental period lasted for 34 days. on day 35, rats from both experimental groups were killed at 08.00, 11.00, 14.00, 24.00 and 02.00 h. food restriction modified the circadian rhythms of acth and corticosterone. in addition, total circulati ... | 1987 | 3035995 |
| [expressing plasmid vectors on the basis of escherichia coli beta-galactosidase gene fragments]. | with the use of synthetic dna fragments, a set of new plasmid vectors has been obtained. the vectors provided high level expression of peptides and small proteins in e. coli as fusions with fragments of beta-galactosidase of various length. these vectors were used to achieve expression of a synthetic gene for a functionally active fragment of bacteriorhodopsin. the yields of hybrid proteins consisting of beta-galactosidase and bacteriorhodopsin fragments were in the range of 5-30% from the total ... | 1987 | 3036168 |
| a high-yield, high-purity elutriation method for preparing human granulocytes demonstrating enhanced experimental lifetimes. | a counterflow centrifugal elutriation procedure for preparing human granulocytes is described. this method permits isolation of 1-2 x 10(9) granulocytes with a yield of 70% +/- 7% from a unit of whole blood in 3 h. these cells are 99% +/- 1% pure and 95-99% viable. when compared to cells prepared by a conventional procedure employing hypotonic lysis to remove red cells, the elutriated cells show enhanced oxidative responses resulting from enhanced longevity. other responses including receptor-me ... | 1987 | 3036986 |
| structure of the 3' region of the human elastin gene: great abundance of alu repetitive sequences and few coding sequences. | two overlapping clones encompassing 8.5 kb of the human elastin gene were isolated from two genomic libraries constructed by partial digestion with either haeiii/alui or sau3a and contained in lambda charon 4a or embl3, respectively. the 6 kb of dna comprising the most 3' portion of the gene were sequenced demonstrating an extremely low coding ratio since only three exons containing a total of 134 translated nucleotides were identified. two exons totaling 78 bp of translated sequences which were ... | 1987 | 3038460 |
| structure of the 3' region of the human elastin gene: great abundance of alu repetitive sequences and few coding sequences. | two overlapping clones encompassing 8.5 kb of the human elastin gene were isolated from two genomic libraries constructed by partial digestion with either haeiii/alui or sau3a and contained in lambda charon 4a or embl3, respectively. the 6 kb of dna comprising the most 3' portion of the gene were sequenced demonstrating an extremely low coding ratio since only three exons containing a total of 134 translated nucleotides were identified. two exons totaling 78 bp of translated sequences which were ... | 1987 | 3038460 |
| complete amino acid sequence of cytochrome c551 from erythrobacter species strain och 114. | the complete amino acid sequence of cytochrome c551 isolated from an aerobic photosynthetic bacterium, erythrobacter sp. strain och 114, was determined. the cytochrome molecule was composed of a total of 119 amino acid residues and its molecular weight including heme was calculated to be 13,235. the sequence was (sequence: see text). its molecular weight indicates that this cytochrome is of the l-type. sequence alignment with other bacterial cytochromes c shows that this cytochrome is similar to ... | 1987 | 3038859 |
| [functional macrophage activity in infection with virulent and avirulent strains of the dysentery microbe]. | the effect of s. flexneri virulent and avirulent (vaccine) strains 2a on the cytoplasmic membrane of mouse macrophages has been studied by evaluating the action of these bacteria on the activity of 5-nucleotidase. the dynamics of the activity of 5-nucleotidase after the introduction of both virulent and avirulent strains has a phasic character with alternating rises and falls in the activity of this enzyme in comparison with the control. s. flexneri vaccine strain produces mainly a stimulating e ... | 1987 | 3039764 |
| evaluation with an iuc::tn10 mutant of the role of aerobactin production in the virulence of shigella flexneri. | to evaluate the role of aerobactin production in the virulence of shigella flexneri, a iuc::tn10 insertion mutant was obtained from strain m90t, a serotype 5 isolate. this mutant was tested for its ability to invade and kill hela cells in monolayers, to elicit keratoconjunctivitis in guinea pigs and to infect ligated segments of rabbit ileal loops. although this mutant did not grow in iron-depleted media, its ability to grow intracellulalry and eventually kill hela cells was unchanged from that ... | 1987 | 3040587 |
| hypersensitivity to shigella outer membrane proteins in mice. | mice sensitized with living sh. flexneri 3a or with outer membrane proteins (omp) derived from these bacteria showed arthus and delayed type hypersensitivity (dth) when injected in the footpad with omp. the delayed reactivity to omp was transferable to normal mice by spleen cells obtained from donor animals which were previously sensitized with living bacteria. | 1987 | 2977277 |
| the pattern of atrial natriuretic peptide release during ventricular tachycardia in man. | 1. plasma levels of atrial natriuretic peptide (anp) are high in many patients with tachycardia, but patterns of release with onset and termination of tachycardia and relationships to haemodynamic recordings are not clear. blood for anp measurements was therefore drawn from the coronary sinus, femoral artery and femoral vein, and simultaneous haemodynamic recordings were made in five patients before, during and after induction of stable ventricular tachycardia for 30 min. 2. tachycardia induced ... | 1987 | 2963719 |
| high affinity aldosterone binding sites (type i receptors) in rat heart. | 1. the use of the receptor stabilizing agent sodium molybdate, and of ru26988 to exclude [3h]-aldosterone binding from type ii glucocorticoid receptors, has enabled the characterization of high affinity type i aldosterone binding sites in rat atrial and ventricular cytosols. 2. in adult male and female rats the affinity of binding (kd 4 degrees c) is approximately 1-2 nmol/l for both atria and ventricles; specificity of binding is similar to that for type i sites in classical aldosterone target ... | 1987 | 2966024 |
| neurosecretion of human hypothalamic immunoreactive beta-endorphin: in vitro regulation by dopamine. | an in vitro perifusion system was used to investigate immunoreactive beta-endorphin (beta-end-i) release from adult human hypothalami in response to dopamine (da) and the da receptor antagonist haloperidol (hal). administration of a 1 microm pulse of da consistently elicited a mean (+/- se) 88 +/- 9% increase (p less than 0.05, n = 5) in beta-end-i release, whereas 1 microm hal had no effect. administration of 1 microm da during three perifusions in which 1 microm hal was added to the medium fai ... | 1987 | 2951610 |
| reversible harmless interruption of testicular blood supply in the ram. | an effective method of interrupting testicular blood flow temporarily and repeatedly in the ram has been developed. blockade of flow has been achieved mechanically by an inflatable occluder placed around the testicular artery at the level of the spermatic cord. the effect of the blockade on total testicular blood supply was investigated using doppler flowmetry and a percutaneous xenon-133 injection method. with both approaches, the blood flow changes after inflation or deflation of the occluders ... | 1987 | 2953703 |
| [a case of severe colitis with positive hemocultures for shigella flexneri]. | 1987 | 2954108 | |
| balloon-dilatable arterial banding prosthesis: experimental study. | two arterial banding prostheses were developed that could be dilated by an intraluminal balloon dilator. one prosthesis consisted of radiopaque umbilical tape with a pleat secured by four 8-0 silk sutures thinly coated with silicone type a medical adhesive. this design was used to band the main pulmonary artery in five dogs, the subclavian artery in five, and the aorta in one. the second prosthesis, a stainless steel fatigued helix encased in a siliconized shield, was used to band the main pulmo ... | 1987 | 2954182 |
| [genetic characteristics and protective properties of neamin-resistant mutant salmonella typhimurium and s. dublin of the nea(r) str(s) and nea(r) str(r) 500 classes]. | the genetic analysis of attenuated mutants, class nea(r) str(s), with the use of bacteriophage p 22 has shown that mutation rendering the mutants resistant to neamine is localized in gene nea a. in experiments with the intraperitoneal infection of mice, the appearance of this mutation in s. typhimurium and s. dublin virulent strains has been found to lead to the decrease of virulence in 100% of clones. on the basis of the data obtained in this investigation, region str-spc in s. typhimurium and ... | 1987 | 2955615 |
| [role of the m. canis carrier state of healthy animals in the epidemiology of zoonotic microsporum infection]. | 1987 | 2956788 | |
| possible cellular mechanism for cerebral vasospasm after experimental subarachnoid hemorrhage in the dog. | this study was undertaken to examine some of the cellular ionic mechanisms responsible for the cerebral vasospasm that occurs as a consequence of subarachnoid hemorrhage (sah). after cisternal injection of autologous blood we documented spasm of the basilar artery upon angiography from 4 to 7 d postictus in six dogs. when these basilar arteries were isolated we observed marked membrane depolarization and enhanced electrical spike activity compared with controls. the slope of the membrane potenti ... | 1987 | 2957393 |
| the use of angiodynography to quantify blood flow in the canine aorta. | previous attempts to quantify blood flow by means of ultrasound have been hampered by inaccurate diameter measurements and failure to account for the parabolic nature of the cross-sectional velocity profile. angiodynography (adg) provides a b-mode image of vessel walls, and the outer flow envelope is constantly monitored to provide phasic diameter measurements throughout the cardiac cycle. frequency shifts are color-coded to provide a real-time visual display of varying velocity patterns across ... | 1987 | 2957515 |
| experimental evaluation of a new albumin-impregnated knitted dacron prosthesis. | the performance of a new albumin-impregnated, knitted vascular graft stored damp in saline was assessed by a comparative study in the canine aorta. knitted grafts interposed into the descending thoracic aorta for 4 weeks included: albumin-coated and stored damp in saline (damp albuminated) (n = 6), albumin-coated and freeze-dried (dry albuminated) (n = 5), collagen-coated (n = 4), and uncoated (n = 4). the results of macroscopic and microscopic evaluation were subjected to one-way analysis of va ... | 1987 | 2962524 |
| the alpha 2-adrenoceptor agonists clonidine, tl99 and dpi enhance vasoconstrictor responses to sympathetic nerve stimulation and noradrenaline in the rat tail artery preparation. | 1. clonidine (1-30 nmol/l) enhanced the vasoconstrictor responses to sympathetic nerve stimulation in isolated perfused artery preparations from the rat tail. higher concentrations of clonidine produced vasoconstriction in most artery preparations and reduced the response to sympathetic nerve stimulation. 2. the enhancing effect of clonidine was not affected by prazosin but was blocked by idazoxan, indicating that it was due to activation of alpha 2-adrenoceptors. 3. the alpha 2-adrenoceptor ago ... | 1987 | 2896082 |
| monoclonal antibodies specific for shigella flexneri lipopolysaccharides: clones binding to type iv, v, and vi antigens, group 3,4 antigen, and an epitope common to all shigella flexneri and shigella dysenteriae type 1 stains. | monoclonal antibodies reactive with shigella flexneri o antigens were generated in both mouse and rat systems. antibody-producing hybridomas were screened in an enzyme-linked immunosorbent assay using chemically defined lipopolysaccharides as antigens, and the epitope specificities were determined with a panel of lipopolysaccharides and synthetic o-antigen-specific glycoconjugates as antigens. to verify the specificity seen in the enzyme-linked immunosorbent assay, the antibodies were used in ag ... | 1987 | 2437036 |
| [genetic characteristics and trends in the spread of a new shigella flexneri subserovar with the antigenic formula iv:7,8]. | as the result of experiments with the conjugation of s. flexneri strains 4 belonging to an unusual subserovar (iv: 7,8) with escherichia coli donor strains k12 hfr c and hfr h, as well as experiments with converting phages iv and 7,8, this new subserovar of s. flexneri 4, similarly to other s. flexneri subserovars, was proved to be the y-variant of shigellae rendered lysogenic by the two above phages. the experiments also revealed that 97.9% of all s. flexneri strains 4 (iv: 7,8) under study pos ... | 1987 | 2437740 |
| the novel carbohydrate epitope l3 is shared by some neural cell adhesion molecules. | the monoclonal l3 antibody reacts with an n-glycosidically linked carbohydrate structure on at least nine glycoproteins of adult mouse brain. three out of the l3 epitope-carrying glycoproteins could be identified as the neural cell adhesion molecules l1 and myelin-associated glycoprotein, and the novel adhesion molecule on glia. expression of the l3 carbohydrate epitope is regulated independently of the protein backbone of these three glycoproteins. based on the observation that out of three fun ... | 1987 | 2438289 |
| characterization of five shigella flexneri variant y-specific monoclonal antibodies using defined saccharides and glycoconjugate antigens. | the structural domains of the shigella flexneri variant y o-antigen epitopes 3,4 have defied definition, despite knowledge of the structure of the linear polysaccharide chain of the lps molecule. the dual epitope designation of group antigen 3,4 is based on absorption data using polyvalent rabbit antisera. five monoclonal antibodies specific for the y antigen, generated after immunization of balb/c mice or lou/c rats, were selected on the basis of elisa by using well-characterized s. flexneri y ... | 1987 | 2438343 |
| structure and biology of shigella flexneri o antigens. | 1987 | 2438412 | |
| increase of enkephalin and decrease of substance p immunoreactivity in the dorsal and ventral striatum of the rat after midbrain 6-hydroxydopamine lesions. | unilateral 6-hydroxydopamine lesions of the mesostriatal dopaminergic system in the rat resulted in a decrease of substance p-immunoreactivity in the ventral striatum, and in a heterogeneously distributed increase of enkephalin-immunoreactivity in the dorsal and ventral striatum and the globus pallidus. the respective decrease and increase are caused by a lower or higher staining intensity of the peptidergic fibers, whereas no changes were found in the cell bodies. | 1987 | 2440523 |
| [enhancement of the therapeutic efficacy of blood substitutes in burn shock]. | 1987 | 2440759 | |
| a new set of monoclonal antibodies to human mhc class ii alpha chains demonstrates that most alpha epitopes are inaccessible on the living cell surface. | when mice were immunized with a mixture of human mhc class ii alpha and beta glycoprotein chains, the predominant antibody response was anti-alpha, and from a subsequent fusion experiment over 60 hybridomas showing anti-alpha activity were generated, compared with 11 anti-beta secretors. these findings contrast with the relative paucity of anti-alpha monoclonals described previously. use of a miniaturized western blot screening protocol was a critical factor in the present study since the anti-a ... | 1987 | 2440796 |
| a new set of monoclonal antibodies to human mhc class ii alpha chains demonstrates that most alpha epitopes are inaccessible on the living cell surface. | when mice were immunized with a mixture of human mhc class ii alpha and beta glycoprotein chains, the predominant antibody response was anti-alpha, and from a subsequent fusion experiment over 60 hybridomas showing anti-alpha activity were generated, compared with 11 anti-beta secretors. these findings contrast with the relative paucity of anti-alpha monoclonals described previously. use of a miniaturized western blot screening protocol was a critical factor in the present study since the anti-a ... | 1987 | 2440796 |
| [determination of serum antibody avidity compared to infectious o-antigenemia in paratyphoid b patients]. | the avidity of serum antibodies depending on infectious o-antigenemia in patients with paratyphoid b has been studied in the course of the disease. the study has revealed a faintly pronounced inverse correlation between the degree of avidity of serum antibodies and the level of infectious antigenemia. the avidity of serum antibodies in paratyphoid b has been found to increase in the course of the disease. a significant dependence of the severity of the disease on the avidity of serum antibodies ... | 1987 | 2441547 |
| [nonspecific and antigen-specific adhesiveness of circulating lymphocytes in dysentery patients undergoing combined therapy using thymalin and indomethacin]. | nonspecific adhesiveness of lymphocytes to polyacrylonitrile fiber and the antigen-specific adhesiveness of t-lymphocytes to shigella flexneri pellicular immunosorbent have been studied in dysentery patients. short courses of treatment with indomethacin, but not with thymalin (the preparation of thymic hormones), used for immunomodulation, have accelerated the normalization of nonspecific lymphocyte adhesiveness during regression of the disease, while indomethacin, in contrast to thymalin, inhib ... | 1987 | 2441548 |
| discrimination of hepatocyte-stimulating activity from human recombinant tumor necrosis factor alpha. | the involvement of tumor necrosis factor alpha (tnf alpha) in the regulation of acute-phase protein synthesis is currently under discussion. in this study the effect of human recombinant tnf alpha on the regulation of the 4 acute-phase proteins alpha 2-macroglobulin, albumin, alpha 1-proteinase inhibitor and alpha 1-acute-phase globulin was investigated in rat hepatocyte primary cultures. no changes in synthesis of any of the 4 proteins were observed. however, an acute-phase response similar to ... | 1987 | 2441999 |
| influence of neuropeptides on airway smooth muscle. | the first bronchoactive (and vasoactive) peptide to be discovered in the lung was isolated and characterized in 1970 from an embryologically related organ, the small intestine. since then, more than 20 additional peptides have been described in lung tissue and their biologic activities investigated. many of these are neuropeptides acting as neurotransmitters or neuromodulators to influence airway, pulmonary vascular, and other functions. more neuropeptides are known to exist in the brain and per ... | 1987 | 2446543 |
| acetylcholine receptor-specific human t-lymphocyte lines. | 1987 | 2446551 | |
| definition of myasthenogenic sites of the human acetylcholine receptor using synthetic peptides. | experimental autoimmune myasthenia gravis (eamg) and antibodies that modulate achrs from cultured human muscle are induced by a disulfide-looped peptide comprising the human acetylcholine receptor (achr) alpha-subunit residues 125-147 (h alpha 125-147). to delineate the essential antigenic requirements for induction of eamg by this peptide, a series of peptides was synthesized: (a) a nonlooped analog (cys 128 replaced by ser) stimulated modulating autoantibodies, induced eamg, and bound antibodi ... | 1987 | 2446556 |
| distributions of certain neuropeptides in the primate thalamus. | the distributions of fibers and terminals immunoreactive for somatostatin (srif), neuropeptide y (npy), substance p (sp) and cholecystokinin octapeptide (cck), were studied in the diencephalon of cynomolgus monkeys. immunoreactivity for all 4 peptides is found in extrinsic afferent fibers innervating the dorsal thalamus, ventral thalamus and epithalamus. the distributions of such fibers are more extensive than previously described and include many relay nuclei in their zones of terminations. sp ... | 1987 | 2446708 |
| "sub-threshold neoplastic states" created in transgenic mice. | several possible scenarios are already suggested by the first series of studies in which a high risk situation with respect to malignant diseases was artificially created by introducing oncogenes in the mouse germ line. only in exceptional cases was the expression of the transgene followed in an obligatory way by cancerous proliferation. the usual situation appears now to be that somatic cells expressing the transgene have only a higher probability than normal cells of undergoing a subsequent tr ... | 1987 | 2835726 |
| "sub-threshold neoplastic states" created in transgenic mice. | several possible scenarios are already suggested by the first series of studies in which a high risk situation with respect to malignant diseases was artificially created by introducing oncogenes in the mouse germ line. only in exceptional cases was the expression of the transgene followed in an obligatory way by cancerous proliferation. the usual situation appears now to be that somatic cells expressing the transgene have only a higher probability than normal cells of undergoing a subsequent tr ... | 1987 | 2835726 |
| flanking and internal regions of chromosomal genes mediating aerobactin iron uptake systems in enteroinvasive escherichia coli and shigella flexneri. | we have investigated the presence of the aerobactin system and the location of the aerobactin genes in enteroinvasive strains of escherichia coli. also, we cloned the aerobactin region and its flanking sequences from the chromosome of a strain of shigella flexneri and compared the molecular organization of the aerobactin genes in the two genera. of the 11 enteroinvasive e. coli strains studied, 5 possessed the aerobactin genes, which were located on the chromosome in each case. these strains pro ... | 1987 | 2832521 |
| antiviral chemotherapy and prophylaxis of viral respiratory disease. | respiratory viruses continue to be major causes of morbidity and mortality. currently available chemotherapy is limited to oral amantadine for uncomplicated influenza a and aerosolized ribavirin for respiratory syncytial virus (rsv) infections. amantadine is also efficacious for chemoprophylaxis of influenza a virus infections. rimantadine has similar clinical efficacy and is better tolerated than amantadine. aerosolized ribavirin may be useful in the treatment of serious respiratory illness cau ... | 1987 | 2446820 |
| theophylline's inhibition of antigen-induced contraction of human parenchymal strips is independent of adenosine antagonism. | the effects of theophylline, and adenosine antagonist, and enprofylline, reported to lack adenosine antagonism, were studied using antigen-induced contraction of sensitized human lung parenchymal strips. cumulative addition of adenosine usually resulted in a small concentration-dependent contraction, which was antagonized by theophylline, 100 microm. antigen-induced contraction was reduced by pretreatment with adenosine, 10 microm, but not by adenosine addition after antigen challenge. dipyridam ... | 1987 | 2446888 |
| structural homology of human complement component c8 gamma and plasma protein hc: identity of the cysteine bond pattern. | anti-c8 alpha-gamma specific antibodies were used to isolate cdna clones from a human liver expression library. antibodies affinity-purified on the expressed hybrid protein of one clone bound exclusively to the gamma-chain of reduced c8 alpha-gamma. this clone, as well as a second full length cdna clone obtained by hybridization screening, were sequenced and the complete primary structure for c8 gamma was established. cyanogen bromide cleavage of c8 alpha-gamma released a 12 kda carboxy-terminal ... | 1987 | 2447883 |
| effects of light- and dark-rearing on the postnatal development of gaba receptor sites in the chick optic lobe. | to investigate the ability of gaba receptor sites to undergo environmental-dependent plastic changes, the postnatal developmental pattern of gaba receptors was studied under different levels of light stimulation, i.e. normal-, light- and dark-rearing. at hatching the specific binding of [3h]gaba was 1.74 +/- 0.36 pmol/optic lobe. in normally reared chicks the number of gaba binding sites showed a transient increase with the highest value at the 6th day (7.0 +/- 1.32 pmol/optic lobe). this value ... | 1987 | 2845719 |
| cloned mouse lymphocytes permit analysis of somatic mutations that occur in vivo. | as part of our mouse model of somatic mutation, we have begun to characterize spontaneously occurring hypoxanthine phosphoribosyltransferase (hprt) -deficient mouse lymphocytes. lymphocytes were cloned by in vitro exposure of spleen cells from male c57b1/6 mice to the mitogen concanavalin a, conditioned medium containing lymphocyte growth factors, and thioguanine (tg), in a limiting dilution assay. the 17 tg-resistant clones recovered were all highly deficient in hprt activity and were found by ... | 1987 | 2842875 |
| localization of plasmid loci necessary for the entry of shigella flexneri into hela cells, and characterization of one locus encoding four immunogenic polypeptides. | we have previously cloned a 44 kb fragment from the virulence plasmid of shigella flexneri serotype 5 strain m90t which is capable of restoring invasiveness to an avirulent, plasmidless mutant. this report presents a genetic and physical analysis of tn5 mutations in recombinant clone phs4108. tn5 mutagenesis allowed identification of at least five regions implicated in the entry phenotype. these regions were located on a 20 kb portion of phs4108. expression of the insertion mutants was studied b ... | 1987 | 2846749 |
| metabolic events mediating early killing of host cells infected by shigella flexneri. | j774, a continuous macrophage cell-line, was infected by m90t, an invasive isolate of shigella flexneri serotype 5 and bs176, its non invasive derivative--which does not harbor the 220 kbase virulence plasmid pwr100. killing of host cells by intracellular m90t, commenced one hour after infection and was completed by 4 hours. intracellular bs176 did not kill cells during the same period. cell protein biosynthesis was totally inhibited by both strains within 2 hours of infection thus indicating th ... | 1987 | 2848171 |
| bw a575c, a chemically novel agent with angiotensin converting enzyme inhibitor and beta-adrenoceptor-blocking properties. | bw a575c (n-(1-(s)-carboxy-5-[4(3-isopropylamino-2-(r, s)-hydroxypropoxy)indole-2- carboxamido]pentyl)-(r, s)-alanyl-(s)-proline) is a chemically novel agent which exhibits in a single molecule both angiotensin converting enzyme (ace) inhibition and beta-adrenoceptor-blocking properties. bw a575c produced a competitive blockade of heart rate responses to isoprenaline in a guinea-pig right atrial preparation (pkb 7.18 +/- 0.05, cf. pindolol 8.9 +/- 0.7). bw a575c inhibited a partially purified pr ... | 1987 | 2882805 |
| glutathione metabolizing enzyme activities in human thyroid. | glutathione peroxidases, glutathione transferase, glutathione reductase and gamma-glutamyl transpeptidase activities were analyzed in human thyroid tissues obtained from 17 patients undergoing resectional surgery because of a malignancy. it was deduced, from measurements of glutathione peroxidase activity with both h202 and cumene hydroperoxide, that thyroid contains only the selenium enzyme. the absence of selenium independent glutathione peroxidase activity in thyroid was confirmed with gel fi ... | 1987 | 2883074 |
| a competitive receptor binding radioassay for beta-1 and beta-2 adrenergic agents. | a rapid and sensitive competitive receptor binding assay for beta-1 and beta-2 adrenergic binding for adrenergic agents has been developed. the steps that are critical for the success of the assay are given in detail so that the assay can be set up in any routine laboratory with relative ease. the rationale behind the use of specific reagents is discussed. the assay requires microgram quantities of test compound, a radiolabeled specific beta adrenergic antagonist [3h]dihydroalprenolol (dha), and ... | 1987 | 2883662 |
| enhancement of contractile responses to partial alpha-adrenoceptor agonists during warming in rat aorta. | we examined the effects of warming on the contractile responses to full and partial alpha-adrenoceptor agonists in rat aorta. the contractions elicited by norepinephrine and methoxamine were not affected during warming (40 degrees c, 42 degrees c), whereas those induced by clonidine and st 587 were significantly enhanced. kcl-induced contractions of rat aorta were not affected by warming. the dissociation constants of clonidine and st 587 at 40 degrees c were not different from those at 37 degre ... | 1987 | 2895427 |
| plasma levels of gastrin, somatostatin, and cholecystokinin immunoreactivity during pregnancy and lactation in dogs. | plasma levels of gastrinlike, somatostatinlike, and cholecystokininlike immunoreactivity were determined by radioimmunoassay in peripheral blood from 11 beagles during pregnancy and in response to suckling during lactation. cholecystokinin was determined in pooled and in individual plasma samples after separation of cholecystokinin and gastrin by high-performance liquid chromatography. gastrin and somatostatin levels were significantly increased during the 8-wk-long dog pregnancy; peak values we ... | 1987 | 2880780 |
| media for isolating shigella. | 1987 | 2881104 | |
| beta-blockade reverses regional dysfunction in ischemic myocardium. | to determine the protective effect of oxprenolol-induced beta-blockade on the compromised myocardium (critical constriction of the left anterior descending coronary artery) against the adverse effect of high concentrations of halothane, halothane dose-response curves were obtained in six dogs in each of three phases: preconstriction (control), critical constriction, and critical constriction with the addition of 0.3 mg/kg intravenous oxprenolol. the extent of depression of ventricular function w ... | 1987 | 2886076 |
| composite effects of actinonin when inhibiting enkephalin-degrading enzymes. | actinonin which has been found to be an inhibitor of aminopeptidase m (ec 3.4.11.2) also inhibited enkephalinase a (ec 3.4.24.11) and enkephalin aminopeptidase which were partially purified from the corpus striatum membrane of guinea-pig brain. the ic50 values were 5.6 microm for enkephalinase a and 0.39 microm for enkephalin aminopeptidase. actinonin also inhibited with an ic50 value of 1.1 microm dipeptidyl aminopeptidase tested on whole brain homogenate of rats in the presence of thiorphan an ... | 1987 | 2886348 |
| azodisalicylate (azodisal sodium) causes intestinal secretion. comparative study of the effect of azodisalicylate, sulfasalazine, 5-aminosalicylic acid and sulfapyridine on the water and electrolyte transfer and the morphology of the rat ileum and colon in vivo. | azodisalicylate (ads) is one of the possible successors of sulfasalazine in the treatment of ulcerative colitis. the following results were obtained when comparing the influence of ads on net water and electrolyte transfer in tied-off loops of the rat ileum and colon in vivo with sulfsalazine, 5-aminosalicylic acid, and sulfapyridine. (1) sulfasalazine, 5-aminosalicylic acid and sulfapyridine had no effect on water and electrolyte transfer of the healthy intestinal mucosa in concentrations up to ... | 1987 | 2887473 |
| activation of a common effector system by different brain neurotransmitter receptors in xenopus oocytes. | xenopus oocytes possess 'native' muscarinic receptors, which give rise to oscillatory chloride currents; similar responses are elicited by activation of foreign receptors to serotonin, glutamate and noradrenaline, expressed in oocytes after injection of messenger rna from rat brain. when low concentrations of two agonists are applied together, the combined response is greater than would be expected from the sum of the responses to each agonist applied alone. potentiation of acetylcholine by sero ... | 1987 | 2888117 |
| identification of recessive mutations at human cancer loci. | 1987 | 2820528 |