Publications

characterization of size-dependent exchange of peg molecules between the blood and extravascular space in the pig.	different sized polyethylene glycols (pegs) have been used as probe molecules in studies of size-dependent permeation through the intestinal wall and the glomerular membranes. we have curve-fitted a three-compartment model to the urinary recovery data following intravenous injection of different sized peg molecules in the pig. the rate constants to and from the extravascular space demonstrate a strong size-dependent selectivity for peg molecules less than 502 da, but the rate constants to the ur ...	1988	3370951
trichosanthin, alpha-momorcharin and beta-momorcharin: identity of abortifacient and ribosome-inactivating proteins.	the abortifacient proteins trichosanthin, alpha-momorcharin and beta-momorcharin at nm concentrations inhibit cell-free protein synthesis. the momorcharins and the ribosome-inactivating proteins isolated from momordica charantia seeds cross-react with the respective antisera. the ribosome-inactivating proteins saporins, pokeweed antiviral protein (pap) and, to a lesser extent, gelonin have abortifacient activity on pregnant mice.	1988	3372132
ocular toxoplasmosis in immunosuppressed nonhuman primates.	to investigate the role of cellular immunodeficiency in recurrent toxoplasmic retinochoroiditis, six cynomolgus monkeys (macaca fascicularis) with healed toxoplasmic lesions of the retina were immunosuppressed by total lymphoid irradiation. three months prior to irradiation 30,000 toxoplasma gondii organisms of the beverley strain had been inoculated onto the macula of eye in each monkey via a pars plana approach. toxoplasmic retinochoroiditis developed in each animal, and lesions were allowed t ...	1988	3372160
anticonvulsant effects of dextrorphan in rats: possible involvement in dextromethorphan-induced seizure protection.	the major metabolite of the non-opioid anticonvulsant/antitussive dextromethorphan is dextrorphan. in the present study, the effects of dextrorphan were determined in an experimental model of seizure activity (maximal electroshock convulsions) (mes). subcutaneous administration of dextrorphan produced dose-related blockade of tonic hindlimb extension (the) and a decrease in the duration of tonic forelimb extension (tfe). the anticonvulsant effect of dextrorphan was linear and maximally efficacio ...	1988	3374269
fiberoptic angioscopy of cardiac chambers, valves, and great vessels using a guiding balloon catheter in dogs.	the applicability of fiberoptic angioscopy with a guiding balloon catheter to observe the cardiac chambers, valves and the great vessels was examined in anesthetized dogs. a no. 11 french guiding balloon catheter (balloon diameter 50 french) was introduced through either the right jugular vein or the right femoral vein into the right heart, and then a fiberscope (4.3 french in diameter) was introduced through the catheter into the right heart in 10 dogs. the balloon was inflated with air and gen ...	1988	3376848
[acute hepatitis: prevention and treatment].		1988	3377335
electrophysiological effects of cocaine and procaine on dorsal raphe serotonin neurons.	the effects of cocaine and procaine on the spontaneous activity of serotonin (5-hydroxytryptamine, 5-ht) neurons in the dorsal raphe nucleus of the chloral hydrate anesthetized male rat were characterized using extracellular single-unit and microiontophoretic recording techniques. intravenous cocaine elicited a potent, dose-dependent inhibition of 5-ht cell firing with a cumulative dose to 50% inhibition of cell firing of 0.66 +/- 0.11 mg/kg (means +/- s.e.m.; n = 17). in contrast, systemic admi ...	1988	3384007
antibody response of an elderly population to a supplemental dose of influenza b vaccine.	recent reports have suggested that the antibody response of elderly persons to standard doses of influenza vaccine is depressed. we examined the effect of an additional threefold dose of influenza b vaccine on the antibody response in elderly, ambulatory veterans. one hundred thirty-one male subjects aged 70 years and older were randomized to receive one of three influenza vaccine regimens: group i received standard trivalent influenza vaccine containing 15 micrograms of b/ussr/100/83 in one arm ...	1988	3385111
[study of the effect of antitumor agents on cell cycle traverse by flow cytometry].	the effect of antitumor agents on the cell cycle traverse of cultured fl cells, a monolayer culture line derived from human amnion, was investigated by flow cytometry (fcm) in combination with simultaneous determination of total cell number, viability, mitotic index and labeling index by [3h]tdr. the results obtained can be summarized as follows: antimetabolites prolonged the duration of the s phase at a low dose, while at a high dose they decreased the rate of progression from the g1 to s phase ...	1988	3389824
taurine prevention of calcium paradox-related damage in cardiac muscle. its regulatory action on intracellular cation contents.	the present study was designed to investigate in chick heart whether oral pretreatment with taurine or taurine added directly to the perfusate has any effect upon calcium paradox-induced heart failure. in both protocols, taurine significantly reduced the mechanical dysfunction resulting from the calcium paradox. taurine pretreatment partially inhibited the excess accumulation of calcium in the myocardium that occurs upon calcium repletion, and microscopy revealed almost normal structure. this pr ...	1988	3390225
cadmium-substituted skeletal troponin c metal binding investigations and sequence assignment of the cadmium-113 resonances.	the binding of cadmium to the calcium binding subunit of skeletal troponin (stnc) has been reinvestigated using direct binding methods and fluorescent derivatives. these data provide straightforward explanations of the observed titration behavior in the 113cd nmr (ellis, p.d., strang, p., and potter, j.d. (1984) j. biol. chem. 259, 10348-10356). further, fluorescent derivatives of skeletal troponin c provide an excellent means of establishing a sequence assignment for the resonances observed in ...	1988	3392014
[the differentiation of bordetella bronchiseptica strains. 7. phenotypic changes in bordetella bronchiseptica through changes of phase].		1988	3395180
representation of forelimb mechanoreceptors within the superior colliculus of the cat.	forelimb mechanoreceptor representation within the superior colliculus was investigated using microelectrode recording in decerebrate or anaesthetized cats. selective activation of different mechanoreceptive fibre classes supplying the distal forelimb revealed a poor representation of all three fibre classes (sa, pc and ra) that innervate the glabrous skin. furthermore, there was no evidence for pacinian (pc) input from the interosseous or wrist region. the tap stimuli which are often needed to ...	1988	3399131
pharmacokinetics and pharmacodynamics of single oral doses of ibopamine, quinidine and their combination in normal man.	the pharmacokinetics and haemodynamics (phono- and impedance cardiography) of single oral doses of 200 mg ibopamine (sk&f 100168), 400 mg quinidine sulphate, and their combination, have been assessed in 6 healthy male volunteers. no significant differences in the mean pharmacokinetic parameters of either drug were seen between the single and combined doses. ibopamine caused an increase in mean estimated stroke volume (sv +29% for the maximum change from baseline and +15% cumulatively over the fi ...	1988	3402527
expression of fibronectin on clonally related transformed and control sublines from an epithelial cell strain and a tumor line of mouse alveolus.	the cellular expression of fibronectin and the importance of fibronectin for the morphology of cultured cells were studied in a lung epithelial cell system. the cells used were clonally related control nal1a, spontaneously transformed nal1as cells of the nal1a cell strain, and transformed clonal sublines of the cell line nal1am and the tumor cell line nul1. fibronectin was detected on the surface of nal1a cells by surface iodination; fibronectin synthesis, secretion, and pericellular accumulatio ...	1988	3409226
a new constrictor device for external induction of a long-term stable and irreversible renal artery stenosis in the dog.	a new externally adjustable constrictor device for renal artery stenosis in the dog is described. the constrictor combines hydraulic and mechanical characteristics, and is connected to the exterior of the animal by a thin catheter. applying hydraulic pressure via the catheter causes the plunger in the device to compress the renal artery to any desired degree of stenosis. a mechanical catch prevents backward movement of the plunger, thus ensuring a stable, irreversible renal artery constriction. ...	1988	3412870
production of a positive direct antiglobulin test due to suramin.	drugs and chemicals containing multiple reactive carbonyl groups have been postulated to produce nonimmunologic positive direct antiglobulin tests (dats) both in vitro and in vivo. suramin sodium, a reverse transcriptase inhibitor that was under investigation as a potential treatment for human immunodeficiency virus infection, has six reactive carbonyl groups. because of suramin's chemical structure, it was hypothesized that the drug might cause a positive dat in vitro and possibly in vivo. sura ...	1988	3415441
[dynamics of folliculogenesis in the mouse ovary after the intrauterine elimination of germ cells by administering cyclophosphamide].	injection of cytostatic--cyclophosphamide--to pregnant mice produces elimination of germ cells in their offspring ovaries. independently on the elimination degree of the germ cells, the amount of growing follicles, as well as dynamics of their growth do not differ from those in the majority of control (intact) animals. in some cases the delay in the rate of the follicular growth is connected with regional outgrowth of the interstitial tissue. this, in its turn, depends on uneven distribution of ...	1988	3415486
metabolism of acetylene and acetaldehyde by rhodococcus rhodochrous.	we studied the ability of a soil bacterium, identified as rhodococcus rhodochrous, to grow on acetylene and to accumulate acetaldehyde. its maximum growth rate on acetylene was obtained at about 30 degrees c (mu = 0.11 h-1) and was independent of the concentration of this gas in air from 0.14 to 16% (v/v). during growth, acetylene was quantitatively transformed to acetaldehyde, ethanol, acetate, co2, and biomass in proportions which varied with culture age and temperature. growth was completely ...	1988	3416237
immunosuppressive substance in the sera of head and neck cancer patients.	serum levels of immunosuppressive substance (is) and immunosuppressive acidic protein (iap) were studied by single radial immunodiffusion in 108 patients with head and neck cancer. mean values of serum is and iap in patients with head and neck cancers were as follows: laryngeal carcinoma, 955 +/- 447 micrograms/ml (is), 604 +/- 324 micrograms/ml (iap); maxillary carcinoma, 896 +/- 384 micrograms/ml (is), 568 +/- 246 micrograms/ml (iap); nasopharyngeal carcinoma, 871 +/- 313 micrograms/ml (is), 6 ...	1988	3416271
the regional variability of enzymes in the brain: relevance to csf enzyme determinations.	the assay of cerebrospinal fluid (csf) enzymes has been suggested for assessing the extent of damage and patient prognosis in cases of brain injury. a potential difficulty associated with using csf enzyme levels as predictors of outcome is the possibility that enzyme concentrations may vary substantially from one brain region to another. we have determined the concentrations of seven enzymes in seven brain regions in the rat and cat. acid phosphatase (acp), aspartate aminotransferase (ast), isoc ...	1988	3416484
experimental contact sensitization with 3,4,5-trichloropyridazine.	the potential of 3,4,5-trichloropyridazine to induce contact sensitization was assessed in the guinea pig maximization test of magnusson and kligman and also in the closed patch test described by buehler. the test material was a 1% solution of 3,4,5-trichloropyridazine in a highly refined mineral oil. the test material elicited moderate to severe irritation when diluted in mineral oil to concentrations of 15-25% and minimal irritation at concentrations of 1-3%. both tests clearly indicated that ...	1988	3416588
effect of retinoids on the proliferation, morphology and expression of glial fibrillary acidic protein of an anaplastic astrocytoma cell line.	we studied the effect of retinoids on the growth and differentiation of a cell line (u 343 mg-a) derived from a human malignant astrocytoma. cultures treated with all-trans or 13-cis retinoic acid showed a dose-dependent inhibition of proliferation and a marked reduction in the mean cell number at the plateau phase of growth (3.5 x 10(6) vs. 1 x 10(7) cells/25 cm2) compared with untreated cultures. at confluence, cells treated with all-trans or 13-cis retinoic acid were contact-inhibited, wherea ...	1988	3417370
effect of gamma irradiation on the protoscoleces of echinococcus granulosus of sheep origin.		1988	3417384
internal pars plana sclerotomy with the contact nd:yag laser: an experimental study.	transvitreal pars plana sclerotomy using the contact nd:yag laser with a sapphire probe was performed on a group of rabbit eyes and compared with a similar procedure performed with a ziegler knife. although the intraocular pressure (iop) was lowered in both groups of animals, the reduction was greater and more lasting in the group on which the nd:yag laser with sapphire probe was used. this technique may have application in cases with extensive peripheral anterior synechiae or where a vitrectomy ...	1988	3417390
optic disc, cup and neuroretinal rim size, configuration and correlations in normal eyes.	four hundred and fifty-seven unselected normal human optic nerve heads of 319 subjects (163 men, 156 women, mean age 42.7 +/- 19.6 years) were evaluated by magnification-corrected morphometry of optic disc photographs. mean optic disc surface measured 2.69 +/- 0.70 mm2 (0.80-5.54 mm2), mean diameter horizontally 1.76 +/- 0.31 mm (0.91-2.61 mm), and vertically 1.92 +/- 0.29 mm (0.96-2.91 mm). the form was slightly vertically oval. optic cup area averaged 0.72 +/- 0.70 (0.00-3.41 mm2), mean horizo ...	1988	3417404
hypotheses on exercise-induced pulmonary hemorrhage in horses.		1988	3417533
crystallization of trimeric recombinant human tumor necrosis factor (cachectin).	crystals of tumor necrosis factor (tnf) have been obtained in two forms. rhombohedral crystals grow in 1.8 to 2.0 m ammonium sulfite, ph 7.8 at 21 degrees c, and tetragonal crystals grow in 2.6 m magnesium sulfate, ph 5.5 at 25 degrees c. analysis of tnf by isoelectric focusing under native and denaturing conditions indicates that tnf molecules exist as trimers in solution. the rhombohedral cachectin crystals belong to space group r3 and have unit cell constants a = b = c = 47.65 a and alpha = b ...	1988	3417634
the fate of venous repair after shock and trauma.	to assess the role of shock and trauma in the subsequent thrombosis of a venous repair, the following experiments were performed. two groups of unsplenectomized dogs were studied: control dogs (gr.c) underwent replacement of a segment of superficial femoral vein by an autogenous vein graft. trauma group (gr.t) dogs were subjected to hypovolemic shock using a modified wiggers' technique and received standardized hindleg trauma. after resuscitation, venous repairs identical to gr.c dogs were perfo ...	1988	3418758
pharmacokinetics of bupivacaine after short and prolonged infusions in conscious dogs.	a conscious dog model was used to study pharmacokinetics of bupivacaine after a short infusion (si) (15 min) and a prolonged infusion (pi) (24 hours). bupivacaine was infused in six mongrel dogs at least 10 days after implantation of femoral arterial and venous catheters. each dog received both the si and the pi in a random crossover design at a one week interval. bupivacaine concentration was measured in serum sampled during the si, during the last hour of the pi, and at frequent intervals duri ...	1988	3421499
experimental evaluation of the mitroflow pericardial heart valve prosthesis. part ii. pathologic examination.	the results of the morphologic and histopathologic examination of 37 bovine pericardial heart valve prostheses that were retrieved after experimental implantation in chacma baboons for periods of one to twelve months are presented in this study. the implanted prostheses consisted of 33 mitroflow valves, size 21 mm, that belonged to four different groups according to the method of preparation of the pericardium (process i to iv) and 4 commercially available ionescu-shiley valves, size 19 mm. all ...	1988	3421507
native genomic blotting: high-resolution mapping of dnase i-hypersensitive sites and protein-dna interactions.	dnase i-hypersensitive sites are observed in the promoter regions of actively expressed genes, potentially active genes, and genes that were once active. we have developed an approach that greatly increases the resolution for mapping these sites by electrophoresing genomic dna on native polyacrylamide gels prior to electroblotting and hybridization. this improved method has been used to scan the promoter and coding region of a cell-cycle-dependent human histone h4 gene with an accuracy of +/-5-1 ...	1988	3422413
ketone-body metabolism in hyperthyroid rats: reduced activity of d-3-hydroxybutyrate dehydrogenase in both liver and heart and of succinyl-coenzyme a: 3-oxoacid coenzyme a-transferase in heart.	the specific activity of d-3-hydroxybutyrate dehydrogenase is reduced by about a third in liver and heart mitochondria of hyperthyroid rats. state 3 respiration is also reduced in isolated mitochondria from the same animals when dl-3-hydroxybutyrate is the substrate. determination of the kinetic parameters of the membrane-bound d-3-hydroxybutyrate dehydrogenase in liver of hyperthyroid rats reveals a decreased in maximal velocity (vmax). the michaelis and dissociation constants of nad+ and d-3-h ...	1988	3422549
[combined resections in the surgery of lung cancer].	operations were performed on 3,313 patients for lung carcinoma in the department of surgery of the lungs and mediastinum of the national research center of surgery, ams ussr, from 1963 till 1.01.90. among these patients 263 (7.9%) were treated by combined operations (resection of the lung together with part of the chest wall, trachea and its bifurcation, pericardium, diaphragm, superior vena cava, pulmonary artery, aorta, and atrium, and also with removal of metastatic lymph nodes of the contral ...	1988	1434353
linear dichroic spectra and fluorescence polarization of biliverdin.		1988	1141595
nursing preferences for clientele.		1988	259418
conduct and utilization of clinical nursing research.		1988	259389
the radiological features of alcoholic ulcero-osteolytic neuropathy in blacks.	the radiological features in the bones and the angiographic changes in the vessels of the lower limbs of 15 chronic alcoholic men are discussed. the distal arteries demonstrate the features of neovascularization. infective and resorptive changes in the bones of the forefeet are described. these features are related to chronic infection or to the toxic effects of the alcohol.	1988	211654
[report on health facilities. 12. midwifery practice at the obstetrical department of kanto teishin hospital: emphasis on health education at a hospital associated with an industry].		1988	259673
guidance on symptoms of illness in babies: a further service from the fsid.		1988	259593
spontaneous rupture of the urinary bladder.		1988	266610
parallel studies of hla antigens in patients with rheumatic heart disease and scleritis: comparisons with three control populations.	parallel studies were carried out on hla antigens in patients with rheumatic heart disease and scleritis. comparison with one control population showed a significant excess of bw15 in both disease samples, while a comparison with two other control populations, showed the excess not to be significant. possible reasons for this discrepancy are discussed, together with the effect on statistical significance of a small percentage of false antigen assignments in one of the samples. a small systematic ...	1988	266606
glomus tumors with special reference to children's feet.		1988	211151
[without vocal cords].		1988	259378
[procedure for immobilization of comminuted fractures of the zygomatic arch].	the authors suggest a method for the reduction and immobilisation comminutive fractures in varus of the zygomatic arch. this procedure consists of the introduction of a franchebois balloon filled with physiological saline into the external space of the manducatory sysarcoses, thereby maintaining the calibe of the zygomato-temporo-malar canal. the procedure falls within the context of the functional treatment of fractures of the facial skeleton.	1988	266263
why patient education. ii. the educational experience--planning, implementing and evaluating teaching for patients with diabetes mellitus.		1987	259961
[determination and application of the so-called biological age].		1987	211834
migrant health question.		1987	259979
do surgeons know about aorn standards?		1987	260641
enzymology--part 1.		1987	260526
effect of the standard on size of the horizontal-vertical illusion.	70 subjects participated in a study designed to replicate the 1968 claim of gardner and long that the horizontal-vertical illusion is larger with the vertical than with the horizontal as standard. the effect did not appear in exp. 1 when subjects made judgments under both the reproduction and graded-series methods but did in a pilot study and in exp. 2, although more strongly with the method of reproduction.	1987	2235250
[nursing in uproar?].		1987	260379
[norwegian health workers in lebanon].		1987	260367
inactivation of bacteria in sewage sludge by gamma radiation.	the survival of certain bacterial cultures suspended in sewage sludge and exposed to gamma-radiation was studied. the inactivation patterns of most of the organisms were significantly different when irradiation was performed using sewage samples collected in the summer and monsoon seasons. the summer sample collected from the anaerobic digestor afforded significant protection to both gram negative and gram positive organisms. this was evident by the increase in dose required to bring about a 6 l ...	1987	15092791
[comparison of different campylobacter-selective media in the study of feces samples of domestic animals].		1987	3426537
neutron scattering determination of the binding of prothrombin to lipid vesicles.	low-angle neutron scattering is used to study the binding of human prothrombin to small single-bilayer vesicles consisting of phosphatidylcholine and phosphatidylserine (1/1 w/w). the radius of gyration of prothrombin indicates that it is an elongated molecule. the vesicles alone were not observed to coalesce, and their molecular weight, outer radius, and average surface area per lipid were respectively (1.6 +/- 0.32) x 10(6), 114 +/- 4 a, and 110 +/- 18 a2. these values were independent of the ...	1987	3427105
modulation of the defective natural killer activity seen in thalassaemia major with desferrioxamine and alpha-interferon.	we previously observed that natural killer (nk) activity toward k562 cells is markedly depressed in patients with beta-thalassaemia major. here we report that these patients also exhibit significantly decreased (p less than 0.005) nk cytotoxicity against human fibroblasts infected with herpes simplex virus-type 1 (hsv-1) and that the amount of alpha-interferon (alpha-ifn) generated during the latter assays is significantly less than normal (p less than 0.005). this decreased production of alpha- ...	1987	3427826
delivery of theophylline into excised human skin from alkanoic acid solutions: a "push-pull" mechanism.	human skin samples are permeable to theophylline delivered from 1.5% solutions in various alkanecarboxylic acids and their mixtures. the respective permeability coefficients of theophylline, calculated from steady-state flux, correlate negatively with the permeability coefficients of the donor carboxylic acids and positively with the excess free energy of theophylline in the donor phase, or "push" effect. an exception is propionic acid which enhances the penetration of theophylline by promoting ...	1987	3430340
localization of source of immunosuppressive factor in the human male reproductive tract.	the source of the immunosuppressive factor (isf) in the human male reproductive tract which suppresses various in vitro immune responses has been identified. we describe here the prostatic localization of this factor, based on investigating the fractions of split ejaculates, ejaculates of individuals with blocked epididymis and the secretions of the prostate obtained by prostatic massage.	1987	3430481
[a new measurement of pulmonary venous compliance by r-synchronized volume loading].		1987	3432757
[reye-like syndrome due to shigella flexneri in a child].		1987	3301584
treatment of bacillary dysentery: a comparison between enoxacin and nalidixic acid.	a prospective randomized study has been conducted in rwanda to compare the efficacy of enoxacin versus nalidixic acid for the treatment of bacillary dysentery in adults. seventeen patients received nalidixic acid and 21 received enoxacin. all isolates (37 shigella, 1 campylobacter) were sensitive in vitro to the two drugs. all patients showed a favourable clinical and bacteriological response and the results obtained with both drugs were comparable. enoxacin seems to be a good alternative to nal ...	1987	3301783
clinical presentation of shigellosis during the 1984 epidemic of bacillary dysentery in west bengal.		1987	3301795
shigellosis in bangladesh: a reply.		1987	3302055
retardation of cell cycle progression in yeast cells recovering from dna damage: a study at the single cell level.	pedigree analyses of individual yeast cells recovering from dna damage were performed and time intervals between morphological landmark events during the cell cycle (bud emergence and cell separation), were recorded for three generations. the associated nuclear behavior was monitored with the aid of dapi staining. the following observations were made: all agents tested (x-rays, mms, ems, mnng, nitrous acid) delayed the first bud emergence after treatment, which indicates inhibition of the initia ...	1987	3302599
production of monospecific antibodies to a low-abundance hepatic membrane protein using nitrocellulose immobilized protein as antigen.	membrane proteins from primary cultures of rat hepatocytes were separated by two-dimensional polyacrylamide gel electrophoresis. the proteins were transferred to nitrocellulose paper which was then dissolved in dimethyl sulfoxide and this mixture was used as a primary immunogen in rabbits. subsequent immunizations were performed using nonsolubilized protein immobilized on nitrocellulose paper. a monospecific polyclonal antibody was generated against a specific mitochondrial membrane protein (mp- ...	1987	3304007
molecular cloning and expression of a synthetic dna coding for the antimicrobial protein of bull seminal plasma.	a dna carrying the coding sequence for the antimicrobial protein from bull seminal plasma (sap) was obtained by enzymic ligation of six synthetic oligonucleotides. the 162 bp synthetic dna fragment was cloned into the c-terminal part of the lacz-gene employing the vector pur289. expression in e. coli in the presence of the inducer isopropylthiogalactoside (iptg) led to the formation of a fusion protein, which was shown by immuno-blotting to contain immuno-reactive antimicrobial protein. approxim ...	1987	3304335
[reaction of the hematopoietic system in mice to the administration of virulent and avirulent strains of shigella flexneri 2a].	the influence of live bacteria and antigenic preparations of virulent and avirulent stains of shigella flexneri 2a on the endogenous splenic colony formation in murine hematopoietic cells has been studied. the different stimulating activity of live microbial cells of virulent and avirulent strains shigella flexneri 2a and their antigenic preparations on the endogenous colony formation has been shown. the effect observed depends on the preparation doses and time of their administration before irr ...	1987	3304434
a new chromogenic test for the detection of urokinase in the genus shigella.	a simple test for detection of urokinase in shigella is described. the test is performed by suspending a loopful of bacteria in 100 microliters of a buffered 1mm solution of benzoyl-beta-alanyl glycyl-arginyl-4-nitroanilide acetate (chromozym u). enzymatic activity is revealed by formation of a yellow colour after 24 h of incubation at 37 degrees c. the test is able to differentiate serotypes of shigella dysenteriae, shigella flexneri, shigella boydii. the results suggest the possibility of incl ...	1987	3306294
chronobiology and epidemiology.	epidemiology and chronobiology are two emerging scientific disciplines of the 20th century. in the united states and certain european and asian countries, epidemiology is a well-developed science; however, in many countries the field is not yet well practiced. too, in several european countries and certain centers in the united states, chronobiology is quite well known and respected as a science. yet, in many countries this field is just now being recognized. although epidemiologists are concern ...	1987	3306581
[morphological changes in the lungs after single whole-body irradiation in rabbits].	morphological changes in rabbit lungs were studied after whole-body nonuniform irradiation (24 gy) the head and the abdomen being shielded. acute pneumonitis was shown to develop between the 17th and the 24th day after irradiation. no pulmonary fibrosis was registered 60 days following irradiation. embolised megakaryocytes were found in the lung capillaries between the 8th and the 24th day after irradiation.	1987	3306764
atrio-ventricular block associated with shigella flexneri infection.		1987	3311739
entry of shigella flexneri into hela cells: evidence for directed phagocytosis involving actin polymerization and myosin accumulation.	the enteroinvasive bacterium shigella flexneri expresses a plasmid-mediated capacity to penetrate into nonphagocytic cells. by using 7-nitrobenz-2-oxa-1,3-diazole-phallacidin (nbd-phallacidin), a fluorescent dye which specifically stains microfilaments, we observed condensations of filamentous actin underneath the plasma membrane of hela cells which interacted with the invasive isolate m90t. as demonstrated by indirect immunofluorescence with the antimyosin monoclonal antibody cc-212, myosin acc ...	1987	3312007
introduction of shigella flexneri 2a type and group antigen genes into oral typhoid vaccine strain salmonella typhi ty21a.	for protection against dysentery caused by shigella flexneri 2a, an in vivo-constructed recombinant plasmid with genes specifying the s. flexneri type and group antigens located near the pro (min 6) and his (min 44) chromosomal markers, respectively, was made and transferred to the gale salmonella typhi strain ty21a. strain ty21a carrying this recombinant plasmid was shown by immunological and biochemical analyses to express the s. flexneri 2a type and group antigens. mice immunized with this va ...	1987	3312013
expression of campylobacter jejuni invasiveness in cell cultures coinfected with other bacteria.	enteroinvasive salmonella, shigella, and escherichia coli strains were found to exert an effect which rendered campylobacter jejuni capable of intracellular localization in epithelial cells in vitro. when monolayers of hep-2 or a-549 cells were challenged with pure cultures of c. jejuni or campylobacter coli, none of the eight strains tested invaded the cells. in contrast, four of these strains were able to localize intracellularly when the cells were challenged with a mixture of campylobacters ...	1987	3312015
[bacillary dysentery 1985].		1987	3313536
hemolytic uremic syndrome associated with shigellosis: report of two cases.	two patients with hemolytic uremic syndrome admitted to udornthani hospital, thailand, were reported. the preceding illness was shigellosis. the stool culture was positive for shigella dysenteriae in one patient and shigella flexneri in another. the management was successful with peritoneal dialysis in one and symptomatic treatment in another. both patients had complete recovery.	1987	3313740
[prospects for using thymalin in treating bacterial intestinal infections].		1987	3314133
technological investigations with attenuated strains of shigella for production of live vaccines. 1. cultivation in a fermenter of an attenuated strain s. flexneri 2a with two markers.	a method was developed for cultivation of the strain shigella flexneri 2a 77 with two attenuated markers. it ensures the preservation of the initial properties of the strain. the regimen for control of dissolved oxygen level was optimized. it was established that yeast extract is a necessary component of the nutritive medium used. the role of the phase and quantity of the inoculum for growth of the bacterial population has been clarified. fresh fermenter culture, applied in rats, shows a high im ...	1987	3314220
shigella bacteremia in adults. a report of five cases and review of the literature.	shigella bacteremia is rare, occurring mainly in children. we describe five adult patients with shigella bacteremia and review data on 22 cases reported in the english-language medical literature. eighteen (67%) of 27 patients had either an underlying condition or were aged older than 65 years. most patients had clinical signs of acute febrile gastroenteritis. however, in six patients, the organism was not isolated from stool. species isolated from blood included shigella flexneri in 11 patients ...	1987	3314766
a 2d-1h-n.m.r. study of some shigella flexneri o-polysaccharides.	a 1h-n.m.r. study of the o-polysaccharides from different types of shigella flexneri has been performed. with the aid of 2d-n.m.r. techniques, namely, j-resolved, spin-spin correlation, and noesy experiments, most of the structural features of these polysaccharides could be deduced. sequences could generally be obtained from the noesy experiments. when using a prolonged mixing time in these experiments, cross-peaks due to spin diffusion from one anomeric proton to the anomeric proton of an adjac ...	1987	3315202
[biological properties of opportunistic microorganisms isolated from patients with acute intestinal diseases].	the comparative study of the incidence of the pathogenicity markers (dnaase, rnaase, phosphatase and hemolytic activity) in shigellae and salmonellae, acknowledged as the causative agents of intestinal infections, and in opportunistic bacteria isolated from the feces of patients with acute intestinal diseases and healthy persons has been made. the study has revealed that dnaase and rnaase activity occurs most frequently in shigella flexneri, in salmonellae and in opportunistic enterobacteria iso ...	1987	3318225
shigellosis at a caribbean resort. hamburger and north american origin as risk factors.	during a three-week period in 1984, 339 tourists and expatriate employees with diarrhea visited the infirmary at a caribbean resort club. epidemiologic studies suggested that over 60% of the 1,893 tourists at the resort club during that time may have been ill. shigella flexneri 4a was isolated from seven of 18 stool specimens. a few cases of diarrheal illness occurred at the resort club before the onset of the outbreak, which was temporally related to illness in a butcher. north american residen ...	1987	3318410
[medico-genetic studies of human provisional organs].		1987	3318529
improved immunohistochemical staining of angiotensin ii in rat brain using affinity purified antibodies.	recent immunohistochemical studies that have sought to detect angiotensin ii/iii (aii/aiii) immunoreactive material in the brain have been forced to rely on a small number of antisera because most aii/aiii antibodies have unexplainably proved unsuitable for immunohistochemistry. although extremely useful tools, these antisera have suffered from high background staining. the purpose of this study was to re-examine and characterize the staining using the most popular aii/aiii antiserum (denise) be ...	1987	3319044
pathogenesis of shigella infections.		1987	3321018
comparative aspects of canine and human atopic dermatitis.		1987	3321301
novel oral vaccines: the effectiveness of cloned gene products on inducing secretory immune responses.		1987	3321939
inhibition of enteropathogenic escherichia coli (strain rdec-1) adherence to rabbit intestinal brush borders by milk immune secretory immunoglobulin a.		1987	3321942
enhancement of the secretory iga response to enteropathogens.		1987	3321943
the relevance of controlled fluoride release with bonded orthodontic appliances.		1987	3323277
[dependence of the antimicrobial effect on the antibiotic concentration in modelling pharmacokinetic profiles in vitro: a comparison of 2 regimens for the single intravenous administration of sisomicin].	kinetics of sisomicin antimicrobial effect on e. coli was studied in an in vitro dynamic model. pharmacokinetic profiles observed in blood of humans treated with different polar dosing schedules i. e. bolus administration and continuous infusion were simulated. the first set of the experiments included simulating the profiles observed at short-term maintenance (0.25-20 min) of relatively high antibiotic levels after bolus administration (cmax = 0.125-10 micrograms/ml). the second set included si ...	1987	3324953
[dependence of the antimicrobial effect on the antibiotic concentration in modelling pharmacokinetic profiles in vitro: a comparison of 2 regimens for the single intravenous administration of sisomicin].	kinetics of sisomicin antimicrobial effect on e. coli was studied in an in vitro dynamic model. pharmacokinetic profiles observed in blood of humans treated with different polar dosing schedules i. e. bolus administration and continuous infusion were simulated. the first set of the experiments included simulating the profiles observed at short-term maintenance (0.25-20 min) of relatively high antibiotic levels after bolus administration (cmax = 0.125-10 micrograms/ml). the second set included si ...	1987	3324953
a new keratinolytic proteinase from clinical isolates of trichophyton mentagrophytes.		1987	3325542
[economic loss from dysentery combined with ascariasis and trichocephaliasis].		1987	3325790
ascending urinary tract infections in rats induced by staphylococcus saprophyticus and proteus mirabilis.	ascending pyelonephritis was induced by staphylococcus saprophyticus and proteus mirabilis both by inoculating the organisms separately and in combination into rat bladders. bacterial cultures of tissue homogenates showed that pyelonephritis by both bacteria occurred significantly more often in rats where the two organisms were instilled concomitantly, suggesting a synergistic virulence between the two species. the antibody response to the different organisms was the same either the bacteria wer ...	1987	3326399
effect of outer membrane proteins of shigella on delayed hypersensitivity in mice to sheep red blood cells.	small doses of outer membrane proteins (omp) of shigella flexneri injected intraperitoneally into mice 1 to 3 days before or 3 days after sensitization of animals with sheep erythrocytes were found to increase delayed hypersensitivity as measured by the footpad reaction. in contrast, administration of higher doses of omp resulted in suppression of hypersensitivity response. cell transfer experiments showed that the spleen cells from sensitized and omp treated mice transferred stimulating and sup ...	1987	3326545
multiple drug-resistance in shigella flexneri isolated from a patient with human immunodeficiency virus.	persistent shigellosis, due to shigella flexneri resistant to multiple antibiotics, developed in a 40-yr-old homosexual man with human immunodeficiency virus infection. the shigella strain demonstrated resistance to ampicillin, tetracycline, and trimethoprim-sulfamethoxazole. although shigella flexneri isolates resistant to trimethoprim-sulfamethoxazole are uncommon in the united states, laboratories should monitor resistance patterns through routine in vitro susceptibility testing.	1987	3326711
phylogeny of lymphocyte heterogeneity: identification and separation of functionally distinct subpopulations of channel catfish lymphocytes with monoclonal antibodies.	the purpose of this study was to identify monoclonal antibodies reactive with channel catfish t cells. since a variety of commercially available anti-human and mouse t cell reagents failed to react with channel catfish leucocytes, the development of anti-fish t cell monoclonal antibodies was undertaken. one of these reagents, designated mab 13c10, reacted with channel catfish surface immunoglobulin negative, but not positive, lymphocytes. this reagent, when used in "panning" protocols, was able ...	1987	3326758
differential clinical features and stool findings in shigellosis and amoebic dysentery.	to obtain information that could assist the clinician to differentiate between shigellosis and amoebic dysentery, we compared clinical features and stool findings in 58 adult male patients in bangladesh. mean values indicated that patients with invasive amoebiasis were older and had a longer prehospital illness, a lower body weight, less frequent fever, a lower haematocrit and a higher white blood cell count than patients with shigellosis. the mean number of faecal leucocytes per mm3 was signifi ...	1987	3328339