Publications

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preparation and characterization of uridinediphosphate-n-acetylmuramyl-l-alanyl-d-glutamyl-meso-2,6-diaminopimelic acid. 19684967095
the optimal mode of transport for swabs obtained from surfaces examined for organisms causing food-borne disease. 19684967143
biosynthesis of bacterial spore coats. 19684967204
ribosomal proteins of bacterial cells: strain- and species-specificity. 19684967207
rapid and sensitive detection of bacteria by gas chromatography.a gas chromatograph fitted with electron capture and flame ionization detectors was employed for the rapid detection of bacteria by analysis for their metabolic products. the presence of proteus vulgaris, streptococcus faecalis, s. liquefaciens, escherichia coli b, bacillus cereus, and b. popilliae was detected in 2 to 4 hr in media inoculated with less than 10(4) cells per ml, whereas a 7- to 12-hr growth period was required for the detection of products formed in cultures of serratia marcescen ...19684967452
antimicrobial activity of lawsonia inermis l. 19684967454
cytotoxic and antifungal agents: their body distribution and tissue affinity. 19684967743
effects in hyperoxia upon microorganisms. i. membrane culture technique for exposing cells directly to test atmospheres.a membrane culture technique was developed for directly exposing microorganisms to test atmospheres. inhibition and killing were calculated from comparisons with air-grown cultures. direct colony counts were used with low inocula. with mass inocula, plate colony counts and optical-density measurements were made on resuspended filter populations. bacteria, including escherichia coli, were more sensitive to oxygen than previously reported. with inocula of a few hundred cells per membrane, five of ...19684967757
azaguanine inhibition of protein synthesis. 3. site of action in hela cells. 19684967763
thiol-group binding of zinc to a beta-lactamase of bacillus cereus: differential effects on enzyme activity with penicillin and cephalosporins as substrates.zinc, which is required for the hydrolysis of cephalosporins by a crude enzyme from bacillus cereus 569, also increased the stability of this activity during storage. a loss in activity of the zinc-activated enzyme which occurred on prolonged hydrolysis of cephalosporin c was not restored by further addition of zinc. the thiol reagents n-ethyl maleimide (nem), iodoacetic acid (iaa), cdcl(2), and p-chloromercuribenzoate, all at 10(-3)m, and iodine at 1.6 x 10(-3)n prevent zinc activation of the " ...19684967769
evidence for a defective protein synthesizing system in dormant spores of bacillus cereus. 19684967802
microcycle sporogenesis of bacillus cereus in a chemically defined medium.a chemically defined medium which allowed germination, outgrowth, and subsequent resporulation of bacillus cereus t spores, without intervening cell division (microcycle sporogenesis), is described. no medium replacement was required. the second-stage spores were heat-stable and had similar germination characteristics and dipicolinic acid content to primary spores. deoxyribonucleic acid (dna) replication began soon after germination and there was a doubling in the dna content of the cells within ...19684967879
purification and properties of uridine diphosphate n-acetylmuramyl-l-alanyl-d-glutamate:meso-2,6-diaminopimelate ligase. 19684967958
[the effect of ionizing radiation on the endogenous respiration of spores from bacillus cereus]. 19684969522
use of the scanning electron microscope for viewing bacteria in soil.scanning electron microscopy was used for viewing bacillus cereus and staphylococcus aureus in three different soils. both organisms were detected in the test soils at an approximate concentration of 10(7) cells per gram of soil; theoretically, the minimal number of microorganisms required for detection with the scanning electron microscope technique was between 10(7) and 10(10) cells per gram of soil. because the concentration of cells was critical, the use of scanning electron microscopy as an ...19684969663
transformation in bacillus cereus 569: a correction of strain designation. 19684969794
lifetime of messenger ribonucleic acid for penicillinase synthesis in several strains of bacilli.1. previous studies of penicillinase synthesis in bacillus licheniformis showed that enzyme synthesis after the addition of actinomycin continues for far longer in the constitutive mutant 749/c than in the parental inducible strain (yudkin, 1966). this result was interpreted as indicating a difference in the lifetime of specific messenger rna in the two strains. other bacilli have now been examined in an attempt to see whether this difference is general. 2. there was no difference in the lifetim ...19684969990
[suppression of penicillinases by compounds similar to penicillins]. 19684970062
fatty acids in the genus bacillus. ii. similarity in the fatty acid compositions of bacillus thuringiensis, bacillus anthracis, and bacillus cereus.the nature and relative abundance of fatty acids produced by two strains each of bacillus thuringiensis and of b. anthracis were studied by gas-liquid chromatography on a 12,000 theoretical plate polyester column capable of partially resolving iso- and anteiso-fatty acids with the same number of carbon atoms. unsaturated fatty acids as the bromo derivatives were separated from the saturated acids and resolved in a short se-30 column by use of programmed-temperature gas chromatography. all four s ...19684970227
comparative thermostability of enzymes from bacillus stearothermophilus and bacillus cereus. 19684970342
a study of immune reactions in cancer. 19684970534
ribosome formation and structure. 19684970633
relationship between protein and ribonucleic acid synthesis during outgrowth of spores of bacillus cereus.the rate of protein and ribonucleic acid (rna) synthesis was examined during the outgrowth of spores of bacillus cereus t in a chemically defined medium. rna synthesis started 2.5 min after the initiation of germination, and protein synthesis after 4 min. addition of a complete amino acid supplement and uracil supported high rates of rna and protein synthesis throughout outgrowth. to determine the relationship between the rate of protein (k) and rna synthesis, the kinetics of formation of variou ...19684970651
[polysynthetic penicillins as inhibitors of penicillinase effect]. 19684970745
a bacteiological assessment of meat, offal and other possible sources of human enteric infections in a bantu township. 19684971025
[studies on bacterial protease. 1. isolation of a protease-producing strain and purification of the enzyme]. 19684971074
changes in the pattern of proteins synthesized during outgrowth and microcycle in bacillus cereus t. 19684971130
infection of bacillus cereus by dna from streptococcus lactis phage. 19684971150
[study on serum and urine concentrations of erythromycin succinate of pyrrolidine-methyl-tetracycline].the serum and urine antibiotic levels in a group of 25 patients were determined after a single administration of a new antibiotic salt: erythromycin succinate of pyrrolidine-methyl-tetracycline. some subjects were treated orally and other intramuscularly. the results obtained showed that this drug gives, both orally and intramuscularly, efficient serum and urine concentrations of tetracycline and erythromycin.196817340794
effect of reduced barometric pressure on water availability related to microbial growth.data obtained from mariner iv indicate that the barometric pressure on mars is considerably lower than previously estimated. current estimates from mariner iv indicate a range from 4 to 7 mb and by near infrared spectroscopy 33-56 mb. inasmuch as the pressure has a marked influence on availability of water, this should affect the existence of martian life. at the maximum temperatures recorded on mars, namely 25 degrees c, a barometric pressure of 30 mb is required for the retention of free water ...196711973845
exobiology and the effect of physical factors on micro-organisms.a study of the action of different physical factors on micro-organisms is necessary for a further development of exobiology. the action of temperature on crystalline preparations of catalase and peroxidase was studied by means of oscillographic polarography. a determination of the height of polarographic waves at the decrease of temperature from 20 degrees c to 0 degrees c has shown that structural elements of the peroxidase molecule connected with the enzymatic activity are more stable with the ...196711973848
[study of specificity of hydrolytic enzyme formed by bac. mycoides hb]. 19674971156
combined induction of penicillinase in bacillus cereus by means of various inducers. i. relative ability of various penicillin derivatives to induce extracellular penicillinase in b. cereus. 19674967290
combined induction of penicillinase in bacillus cereus by means of various inducers. ii. simultaneous action of various penicillin derivatives on extracellular penicillinase formation in b. cereus. 19674967291
spores of microorganisms. xxii. inhibition of the postgerminative development of spores of bacillus cereus by 6-azauracil. 19674965921
studies on the specificity of the enzymic synthesis of polyribonucleotides. 19674965951
purification and properties of the gamma-type beta-lactamase of bacillus cereus. 19674965690
[specificity of cell-injuring reaction (cir) by bacteria--differences in the reaction between cancer and non-cancer cells. 3. time factors in the cir measurement in the y-27-ehrlich ascites cancer cells and effects of the cell concentration]. 19674965718
[specificity of cell-injuring reaction (cir) by bacteria--differences in the reaction between cancer and non-cancer cells. 5. identification of od 260 mmm. substance released from the cell in the y-27-es system]. 19674965719
[examinations on semi-preserved broiling sausages. ii. some properties of isolated aerobic spore formers inportant for food hygiene]. 19674965720
some preliminary observations on the location of esterases in bacillus cereus. 19674965328
[specificity of the cell-injuring reaction (cir) by bacteria. 2. difference between cancer and non-cancer cells in the cir]. 19674965342
biosynthesis of biotin in microorganisms. v. control of vitamer production.use of a yeast-lactobacillus differential microbiological assay permitted investigation into the synthesis of biotin vitamers by a variety of bacteria. a major portion of the biotin activity was found extracellularly. the level of total biotin (assayable with yeast) greatly exceeded the level of true biotin (assayed with lactobacillus). values for intracellular biotin generally showed good agreement between the assays, suggesting the presence of only true biotin within the cells. bioautographic ...19674965364
tracer studies to locate the site of platinum ions within filamentous and inhibited cells of escherichia coli.the distribution of platinum ions within escherichia coli after the induction of filaments with cis-pt(nh(3))(2)cl(4), and after growth inhibition by ptcl(6) (2-), has been determined with radioactive metal compounds ((191)pt, with a half-life of approximately 3 days) by the simple chemical procedure of roberts et al. in the filamentous cells, the platinum metal is associated with metabolic intermediates, nucleic acids, and cytoplasmic proteins; whereas, in inhibited cells, the platinum is combi ...19674965367
dipicolinic acid-less mutants of bacillus cereus. 19674965371
survival of microorganisms in a simulated martian environment. ii. moisture and oxygen requirements for germination of bacillus cereus and bacillus subtilis var. niger spores.the effects of moisture and oxygen concentration on germination of bacillus cereus and b. subtilis var. niger spores were investigated in a simulated martian environment. less moisture was required for germination than for vegetative growth of both organisms. a daily freeze-thaw cycle lowered moisture requirements for spore germination and vegetative growth of both organisms, as compared with a constant 35 c environment. oxygen had a synergistic effect by lowing the moisture requirements for veg ...19674961769
induction of penicillinase with inorganic phosphate.phosphate stimulates penicillinase formation in bacillus cereus 569. the rate of penicillinase synthesis in the presence of 0.3 m phosphate, ph 7.0, is approximately 10-fold greater than that for uninduced cells, while the rate of synthesis in the presence of 0.3 m phosphate and 1 unit/ml of penicillin is approximately fourfold greater than in the presence of penicillin alone. when phosphate-induced cells are transferred to low phosphate medium, the rate of penicillinase synthesis rapidly revert ...19674964486
the metabolism of phosphonates by microorganisms. the transport of aminoethylphosphonic acid in bacillus cereus. 19674963810
thermal penicillinasderepression and temperature dependence of penicillinase production inducible and constitutive strains of bacillus cereus. 19674964018
sensitivity of aerobic micrococcaceae to daq. 19674964037
bacterial variants in the human kidney. 19674964054
the mutagenic effect of prolonged treatment with ethyl methanesulfonate. 19674964634
interference of barbiturates with pyrimidine incorporation. i. amobarbital inhibition of orotate uptake into bacillus cereus. 19674964701
adaptation of populations of bacillus cereus to tetracycline. 19674964702
[the antagonistic action between entomophagic fungi and certain crystallophoric entomopathogenic cereus species]. 19674964906
effect of gamma irradiation on the growth rate of species of bacillus cereus group. 19674965016
studies on filamentous forms of bacillus cereus strain t. 19674965056
the regulation of penicillinase synthesis. 19674965076
partial characterization of the protein labeled in a cell-free system from bacillus cereus 569. 19674965077
nonselective incorporation into sporangium of either "older" or "younger" chromosome of the vegetative cell during sporulation in bacillus cereus.employing bacillus cereus strain 2, we examined the fate of two chromosomes contained in vegetative cells in the course of sporulation. cytological observations and quantitative estimation of deoxyribonucleic acid (dna) confirmed the earlier observations that, during the course of sporulation, one of two chromosomes of the vegetative cell was incorporated into the sporangium and the other disappeared into the medium as the result of cell lysis. log-phase cells, labeled completely with thymine-2- ...19674965196
orded substructure in the cell wall of bacillus cereus. 19674965200
effect of intracellular poly-beta-hydroxybutyrate on the ultraviolet sensitivity of bacillus cereus. 19674959689
x-ray diffraction studies on selected bacterial cell walls.the cell walls of selected bacteria were studied by x-ray diffraction analysis to determine and characterize crystalline components. the walls were isolated by mechanical disruption and purified by enzymatic and washing procedures. the x-ray diffraction lines which appeared from the gram-positive cell walls were shown to be due to the constituent "mucopeptide" fraction. no diffraction lines could be obtained from the gram-negative bacterium studied. the results show that crystallinity is associa ...19674960150
induced rapid release and uptake of phosphate by microorganisms.cultures of bacteria and of mixed microorganisms are able to release actively a large proportion of their phosphorus to the medium in a matter of hours when kept under anoxic conditions. only phosphorus is lost, probably as orthophosphate and apparently mostly from the acid-soluble fraction of the cells. the process, which is completely reversible upon aeration, has a change in rate (for a change of 10 degrees c) of 2 to 2.5.19674959890
ultraviolet irradiation of dna in vitro and in vivo produces a 3d thymine-derived product.a new thymine-derived product was separated from dna irradiated with utlraviolet light in vitro and in vivo. this compound was mistaken to be thymine homodiner (t=t) by other workers because it is chromatographically indistinguishable from t=t in most eluents. it has absorbancy maximums at 312, 312, and 300 millimicrons in neutral, ph 2, and ph 11 aqueous solutions, respectively. when it is irradiated in aqueous solution with wavelengths of 360 and 313 millimicrons its spectrum reverts to one si ...19674960673
symposium on immunosuppressive drugs. comparative actions of some purine analogues on growing bacillus cereus. 19674960737
regulation of extracellular protease production in bacillus cereus.both sporulation and protease production can be inhibited by growing bacillus cereus t in a medium containing a high concentration of a mixture of amino acids. mutants selected for the ability to sporulate in this inhibitory medium were found to produce high levels of protease in the normal and inhibitory media. comparison of the mutant and wild-type enzymes by gel electrophoresis and heat inactivation suggested that they were identical. one of the mutants proved to be a purine-requiring auxotro ...19674960917
fatty acids in the genus bacillus. i. iso- and anteiso-fatty acids as characteristic constituents of lipids in 10 species.fatty acids produced by 22 strains of 10 species of the genus bacillus were analyzed on a very efficient and selective gas-liquid chromatographic column. all of the 10 species, alvei, brevis, cereus, circulans, licheniformis, macerans, megaterium, polymyxa, pumilus, and subtilis, produced eight fatty acids, six branched (anteiso-c(15), anteiso-c(17), iso-c(14), iso-c(15), iso-c(16), and iso-c(17)) and two normal (n-c(14) and n-c(16)). in all cases, the six branched-chain fatty acids made up over ...19674960925
extracellular hemolysins of aerobic sporogenic bacilli.forty-five strains, representing 18 species of the genus bacillus, were surveyed for production of hemolysin against rabbit erythrocytes. broth cultures of b. cereus, b. alvei, and b. laterosporus contained lysins that closely resembled streptolysin o. b. subtilis and a single strain of b. cereus may produce lysins having characteristics different from those of streptolysin o.19674960930
efficiency of endodontic culturing procedures using wet and dry paper points. 19674961037
biochemical studies of bacterial sporulation and germination. v. purine nucleoside phosphorylase of vegetative cells and spores of bacillus cereus. 19674961054
electron microscopic aspects of membrane alterations during bacterial cell lysis. 19674961137
cereolysin: production, purification and partial characterization. 19674961831
instrumental assay of microbial lipase at constant ph.a rapid, accurate method with high sensitivity and reproducibility, and having the advantage of a short incubation period under constant ph, has been developed for routine measurement of microbial lipase. assembled from readily available and economical instrumental components, the apparatus includes a ph meter, a thermoelectric heating and stirring device, a motor-driven burette, and an automatic recorder. the reaction mixture, consisting of 5 ml of a 10% olive oil-gum arabic emulsion, 2 ml of 3 ...19674961930
effect of exogenous substrates on the endogenous respiration of bacteria. 19674962061
comparison of the germination and outgrowth of spores of bacillus cereus and bacillus polymyxa. 19674962189
resistance to nisin and production of nisin-inactivating enzymes by several bacillus species. 19674962191
immunological differentiation between cell walls of streptomycin-sensitive and streptomycin-dependent bacteria. 19674962215
autoradiographic studies of bacterial cell wall replication. i. cell wall growth of bacillus cereus in the presence of chloramphenicol. 19674962235
improved assay method for phospholipase c.a lecithin sol dispersed with deoxycholate was found to be attacked by phospholipase c in the presence of calcium ion more rapidly than were any other lecithin sols. the inorganic phosphate could be released quantitatively from the acid soluble phosphate liberated from lecithin by an excess amount of alkaline phosphatase present in phospholipase c reaction mixture. a simple and accurate assay method for phospholipase c was developed with the sol and the alkaline phosphatase.19674962285
ordered synthesis of proteins during outgrowth of spores of bacillus cereus.the onset of macromolecular synthesis in activated spores of bacillus cereus occurs under conditions in which the amino acids and nucleotides to be used for building proteins and nucleic acids must be derived only from stored pools and turnover of macromolecules of the spore. upon addition of the factors required to initiate germination, (14)c-uracil is incorporated with a lag of 30 to 60 sec; (14)c-amino acids, with a lag of 3 to 4 min. the progression of protein synthesis during germination ha ...19674961412
conversion of ddt to ddd by pathogenic and saprophytic bacteria associated with plants.of 27 microorganisms examined, 23 plant pathogenic and saprophytic bacterial species were found to convert p,p' ddt to p,p' ddd under anaerobic conditions. the range of conversion of ddt (10 micrograms per milliliter) during an incubation of 14 days was from a trace to over 5 micrograms per milliliter, with the majority of the bacteria showing the greatest activity during the final 7-day period. there is evidence that metabolites of ddt other than ddd are also produced.19674961613
treatment of staphylococcal endocarditis. 19674962583
time-lapse cinematography of vancomycin--treated microbial cells. 19674962842
sporicidal properties of some halogens. 19674963102
antigens of bacillus cereus: a comparison of a parent strain, an asporogenic variant and cell fractions. 19674963103
origin and function of penicillinase: a problem in biochemical evolution. 19674963324
determination of dipicolinic acid in bacterial spores by ultraviolet spectrometry of the calcium chelate. 19674963388
a concentration technic applicable to enzyme preparations. 19674963389
aminopeptidase profiles of various bacteria.the aminopeptidase specificity of 24 strains of bacteria was determined fluorometrically by use of a series of alpha-amino acid beta-naphthylamides as substrates. provided that strict control over medium and growth time was adhered to, a reproducible profile of aminopeptidase activity was obtained which could be used for the identification of bacteria.19674963444
some properties of two extracellular beta-lactamases from bacillus cereus 569/h. 19674963457
participation of the lysine pathway in dipicolinic acid synthesis in bacillus cereus t. 19674382288
the role of iron and molecular oxygen in pulcherrimin synthesis by bacteria. 19674968608
[liberation of extracellular penicillinase during the process of enzyme induction in penicillase producing bacteria]. 19674981234
[post-traumatic purulent meningitis due to b. cereus. reply to the opinion of chr. tauchnitz]. 19674969505
cancer and environmental characteristic of the cell. 19674969453
[studies on the reduction of nitrite in bacillus cereus]. 19674973326
[acids compositions of microbial stems belonging to the species b. cereus and b. thuringiensis]. 19674973511
occurrence of leuconostoc and yeasts in confectioners' goods and their interaction. 19674972338
the correlationof a bacterial antigen with cancer. 19674968828
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