Publications

a novel 190 kda calmodulin-binding protein associated with brain actomyosin.	we present evidence for a novel calmodulin-binding protein associated with ca2+-sensitive, non-muscle actomyosin from rabbit brain. gel filtration chromatography of brain actomyosin and sds-page analysis of resulting fractions revealed the presence of a stable complex of a 190 kda polypeptide and calmodulin. furthermore, brain actomyosin was retained on a column of conjugated calmodulin-sepharose 4b and eluted by 6 m urea in the presence of ca2+. further elution with 6m urea plus egta released t ...	1988	3203157
[ontogenetic characteristics of ascorbic acid binding by nerve tissue proteins].	the highest ascorbic acid content has been found in a low-molecular fraction (m. w. less than 6000) of water-soluble proteins from brain tissue homogenates of rats, the lowest one--in a high-molecular fraction (m. w. about 65,000). the content of protein-bound ascorbic acid decreases during postnatal life. this process is accompanied by changes in the ratio of the reduced and oxidated forms of the ascorbic acid in favour of the latter.	1988	3206951
connective tissue remodelling in the ovine cervix during pregnancy and at term.	cervical ripening and dilation in the ewe has been quantitated in terms of the connective tissue content of the tissue and the biosynthesis of the macromolecular components which make up the extracellular matrix of this tissue. during pregnancy there is a marked increase in the total tissue mass of the cervix along with the total mass of hydroxyproline (collagen) and hexuronate (proteoglycans and hyaluronate). the concentration of hydroxyproline of the cervix based on wet weight of the tissue de ...	1988	3208512
connective tissue remodelling in the ovine cervix during pregnancy and at term.	cervical ripening and dilation in the ewe has been quantitated in terms of the connective tissue content of the tissue and the biosynthesis of the macromolecular components which make up the extracellular matrix of this tissue. during pregnancy there is a marked increase in the total tissue mass of the cervix along with the total mass of hydroxyproline (collagen) and hexuronate (proteoglycans and hyaluronate). the concentration of hydroxyproline of the cervix based on wet weight of the tissue de ...	1988	3208512
paracrine control of immature sertoli cells by adult germ cells, in the rat (an in vitro study). cell-cell interactions within the testis.	enriched populations of germ cells prepared from adult rats were found to influence 20-day-old rat sertoli cell secretory activity by stimulating androgen-binding protein (abp) and inhibiting oestradiol-17 beta production in the presence of follicle-stimulating hormone (fsh) as well as of dibutyryl cyclic amp (dbcamp). among the different populations tested in coculture, pachytene spermatocytes were the most effective at stimulating abp and inhibiting oestradiol production, whereas early spermat ...	1988	3208988
gut-associated lymphoid tissue in the large intestine of calves. ii. electron microscopy.	scanning electron microscopy of lymphoid tissue in the large intestine of three germfree calves (age 3, 6, and 7 days) revealed two different units: propria nodules and lymphoglandular complexes (lgc). propria nodules had lymphoid tissue predominantly in lamina propria and were covered by distinct follicle-associated epithelium which lacked goblet cells; nodules were surrounded by wide crypts, which were also lined by follicle-associated epithelium towards the luminal side. lymphoglandular compl ...	1988	3212895
[facilitatory effect of trifluoperazine on adrenergic transmitter release from rat portal vein].		1988	3218532
arautp-affi-gel 10: a novel affinity absorbent for the specific purification of dna polymerase alpha-primase.	for the specific purification of eukaryotic dna-dependent dna polymerase alpha, we prepared two novel affinity resins bearing 5-(e)-(4-aminostyryl) arautp as a ligand. one of them was arautp-sepharose 4b which was coupled directly with the ligand and the other was arautp-affi-gel 10 which was coupled with the ligand through a spacer. no dna polymerase alpha-primase activity from cherry salmon (oncorhynchus masou) testes was bound on the arautp-sepharose 4b in all cases examined. on the other han ...	1988	3218743
affinity of microorganisms of the genus ureaplasma to the reproductive organs of cattle.	the purpose of this work was to define more precisely the role of ureaplasma organisms in the aetiology of granular vulvovaginitis and balanoposthitis (gvvbp) of cattle. to contribute to this question the frequency and degree of infection with ureaplasmas in two main groups of cattle was taken into account: (a) in cattle with symptoms of the mentioned disease, (b) in cattle without clinical symptoms. the samples of semen from 301 sires with symptoms of gvvbp and from 43 healthy sires as also vag ...	1988	3219829
epidemiology of hiv infection in africa.	hiv infection in africa is primarily acquired through heterosexual activity, accounting for up to 80% of cases. prostitutes and sexually promiscuous individuals are at particularly high risk of acquiring infection via this route. in the general population, women between the ages of 18 and 30 years are at increased risk of transmission. the role of cofactors, particularly concurrent sexually transmitted diseases (stds), appears to facilitate heterosexual spread. these groups represent opportuniti ...	1988	3225742
age and lpr dependent induction of increased sensitivity to the toxic effects of lipopolysaccharide and indomethacin in mrl mice: evidence for res activation during disease progression.	injection of 4.5-6 month old female mrl-lpr/lpr mice with 200-250 micrograms lipopolysaccharide led to the death of the animals within two days. treatment of age matched female control mice (mrl-+/+) or young (3 month) mrl-lpr/lpr mice did not lead to similar toxicity. however, pretreatment of young mice with the pyridine extract of residue of corynebacterium parvum (alternatively designated propionibacterium acnes) prior to lipopolysaccharide injection, led to the rapid death of 100% of the mic ...	1988	3230580
[the genetic activity of an alkaline solution of formaldehyde].	toxicity and genetic activity of disinfectant consisting of 1.5% paraform and 0.5% naoh were studied. it was found that the preparation caused a weak mutagenic effect on crepis capillaris and chlorella roots, but had no effect in the ames test and gonadal cells of mammals. the studies of mutagenic effect of the preparation on somatic cells of white mice show limits of the cytogenetic effect at the level of 3.5 g/kg (1/10 ld50).	1988	3232201
individual free fatty acids and lactate uptake in the human heart during severe sepsis.	coronary haemodynamics and myocardial metabolism of nonesterified fatty acids (nefa) and lactate were studied in 11 patients with severe sepsis, and compared to 10 control subjects. coronary sinus blood flow was evaluated by thermodilution. arterial and coronary sinus blood samples were collected for the measurement of lactate and total and individual nefa concentrations both in septic and control patients. there was an increase in lactate and total nefa arterial concentrations with a marked inc ...	1988	3232957
an increase of collagen biosynthesis precedes other symptoms of ethanol-induced liver damage in rats.	treatment of rats with 10% ethanol for 6 months induced a 3-4-fold increase of [3h]proline incorporation into liver collagen and enhanced the level of prolyl hydroxylase activity in liver and serum of intoxicated rats. several diagnostic tests used to assess liver disease failed to demonstrate any symptom of liver damage. therefore, increased collagen biosynthesis appears to precede other symptoms of liver damage induced by ethanol.	1988	3234225
[infection by the human immunodeficiency virus and the lymphadenopathy clinical picture. clinico-developmental study of 86 patients].		1988	3247624
[infection by the human immunodeficiency virus and the lymphadenopathy clinical picture. clinico-developmental study of 86 patients].		1988	3247624
[effect of the growth rate on the activity of nitrogen metabolism enzymes in chemostat cultures of candida diddensii and candida tropicalis].		1988	3247869
effects of an extract of feverfew (tanacetum parthenium) on arachidonic acid metabolism in human blood platelets.	it has been suggested that feverfew extracts (fe) inhibit platelet behaviour via effects on platelet sulphydryl (sh) groups. in the present study we found evidence that fe inhibits uptake as well as liberation of arachidonic acid (aa) into/from platelet membrane phospholipids (pl).	1988	3248111
depression of intestinal epithelial cell production rate by hindgut bypass in rats.	influence of hindgut bypass on the epithelial cell production rates of the small and large intestine was tested in rats. hindgut bypass decreased cryptal cell production rates of the jejunum, ileum, cecum, and distal colon by 40-65%. these results suggest that the entrance of ileal effluent into the hindgut and, presumably, the resultant microbial production of short-chain fatty acids are required to maintain the normal epithelial cell proliferation not only in the hindgut but also in the small ...	1988	3249918
a model for simultaneous study of pressure and electric events in the rabbit sphincter of oddi and duodenum.	an animal experimental model featuring simultaneous recordings of electromyography and pressure from the sphincter of oddi (so) and duodenum is demonstrated. on the basis of data from 10 rabbits, pressure recordings from the so were shown to have phasic activity with an average basic pressure peak interval of 2.6 to 3.5 sec. histographic analysis of so pressure recordings showed a multimodal appearance, suggesting that the activity is paced. a substantial amount of overlap between so and duodena ...	1988	3249919
significance of heel pad confinement for the shock absorption at heel strike.	shock absorption (sa) is a simple way to reduce the body load and can be used in the prevention and treatment of injuries. the heel pad is the most important shock absorber in the shoe heel complex. the purpose of this study was to investigate whether the sa at heel strike can be increased by heel support in people and shoes with high or low sa. the impact forces at heel strike were measured on an amti (r) force platform. fourteen legs were tested in seven persons (nine with normal and five with ...	1988	3253241
significance of heel pad confinement for the shock absorption at heel strike.	shock absorption (sa) is a simple way to reduce the body load and can be used in the prevention and treatment of injuries. the heel pad is the most important shock absorber in the shoe heel complex. the purpose of this study was to investigate whether the sa at heel strike can be increased by heel support in people and shoes with high or low sa. the impact forces at heel strike were measured on an amti (r) force platform. fourteen legs were tested in seven persons (nine with normal and five with ...	1988	3253241
phagocytic and bactericidal properties of macrophages of mice immunized with outer membrane proteins (omp) of shigella.	macrophages obtained from peritoneal exudates of mice immunized with the single doses (1 and 5 micrograms) of omp were shown to have stronger phagocytic as well as bactericidal properties in relation to shigella flexneri bacilli than nonactivated macrophages. macrophages from the animals immunized with 10, 20 and 30 micrograms omp doses showed phagocytic and bactericidal properties similar to those of nonactivated macrophages while the immunization with the dose 50 micrograms resulted in their s ...	1988	3254119
the role of serum factors and influence of intravenous immunoglobulins on phagocytosis of campylobacter species.		1988	3255396
the relationship between blood and fertility parameters in post partum dairy cows.		1988	3256237
the relationship between blood and fertility parameters in post partum dairy cows.		1988	3256237
the effects of anions on fluid reabsorption from the proximal convoluted tubule of the rat.	1. fluid reabsorption from surface proximal tubules of the rat was measured in vivo using stationary microperfusion techniques. reabsorptive rate (jv) was measured from droplets containing chloride as the main reabsorbable anion and when chloride was substituted by bromide, iodide, nitrate, acetate, isethionate or methylsulphate in either the tubular lumen alone or in both lumen and peritubular capillaries. 2. in tubules with an intact blood supply, droplet volume decreased in a manner best desc ...	1988	3256612
reticuloendothelial clearance and splenic mononuclear cell populations after resection and autotransplantation.	although the preservation of splenic tissue may prevent overwhelming infection after splenectomy, the degree of protection conferred by small remnants has not been optimal. we investigated whether either splenic reticuloendothelial clearance of a blood flow-dependent colloid or macrophage and t-cell populations might be altered by resection or autotransplantation of the spleen. our results have shown that bloodstream reticuloendothelial clearance of technetium 99m sulfur colloid is not impaired ...	1988	3257658
hydrogen peroxide-mediated inhibition of t-cell response to mitogens is a result of direct action on t cells.	hydrogen peroxide, a reactive oxygen intermediate produced by activated neutrophils, has been shown to inhibit the response of human t lymphocytes to mitogens and alloantigens. since hydrogen peroxide is known to react with iron and to induce lipid peroxidation, we compared the effects of hydrogen peroxide and a lipid peroxidation product, malondialdehyde, on the response of human peripheral blood mononuclear cells to t-cell mitogens. peripheral blood mononuclear cells pretreated with 1 mmol/l o ...	1988	3257684
lymphocyte chimerism in a full allogeneic composite tissue (rat-limb) allograft model prolonged with cyclosporine.	lew recipients of aci vascularized hind limb allografts were analyzed for lymphoid chimerism by a complement-dependent cytotoxicity assay using antisera produced across this strain combination. in assessing the technique, two lew recipients of sublethal irradiation (400 rad), aci bone-marrow allografts, and csa exhibited mixed lymphoid chimerism 23 days posttransplant. short-term csa-treated cta recipients that were assayed at various times following transplantation and underwent subacute reject ...	1988	3259042
human cryptosporidiosis in north queensland.	during a 12-month period, feces from 780 persons from the townsville region were evaluated by the kinyoun acid-fast strain, and 36 (4.6%) immunocompetent patients were found to have cryptosporidium oocysts. twenty-five index cases were identified; 13 (8.6%) cases from 151 patients were from palm island, an isolated aboriginal community in the wet tropics and 12 (1.9%) cases from 629 patients were from the dry tropics of townsville. all 11 secondary cases were associated with a person-to-person o ...	1988	3264149
electrophysiologic effects of aspirin on myocardium: interaction with catecholamine.		1988	3269682
electrophysiologic effects of aspirin on myocardium: interaction with catecholamine.		1988	3269682
contamination of freshly slaughtered pig carcasses with human pathogenic yersinia enterocolitica.	evidence is presented for the extent of contamination of freshly slaughtered pig carscasses with human pathogenic yersinia enterocolitica and shows the significance of faecal contamination as a source of infection. swab samples collected from the rectum and the surface of a total of 1458 pig carcasses were examined for the presence of human pathogenic y. enterocolitica. y. enterocolitica, biovar iv, serogroup 0:3, were isolated from the rectum of 360 pigs (24.7%). the organism was isolated from ...	1988	3275322
experimental portal fibrosis produced by intraportal injection of killed nonpathogenic escherichia coli in rabbits.	an attempt was made to develop an animal model for the study of the etiology of noncirrhotic portal fibrosis or idiopathic portal hypertension based on the assumption that it is related to chronic abdominal infection. rabbits were given killed nonpathogenic escherichia coli intraportally or intravenously. the animals to which a mixture of killed e. coli and rabbit antiserum (aggregated e. coli) was given intraportally developed remarkable histologic changes in the liver. the early inflammatory r ...	1988	3276575
shigella bacteremia.		1988	3277577
a basic replicon of virulence-associated plasmids of shigella spp. and enteroinvasive escherichia coli is homologous with a basic replicon in plasmids of incf groups.	shigella species and enteroinvasive escherichia coli strains carry a large (120- to 140-megadalton) plasmid called pinv, which contains genes essential for the invasiveness of these pathogens. hybridization with specific probes derived from the repfic and repfib replicons of the incf1 ent plasmid p307 showed that pinvs present in 35 clinical isolates are homologous with repfic but not repfib, regardless of the serogroup of the shigella or e. coli strain. repfic of p307, in turn, is very similar ...	1988	3278983
combined parenteral and oral immunization results in an enhanced mucosal immunoglobulin a response to shigella flexneri.	achieving a vigorous secretory immunoglobulin a (iga) response in intestinal secretions usually requires multiple doses of antigen given orally, while systemic immunity is more easily attained by parenteral immunization. this study examines the role of combined parenteral and oral immunizations to enhance the early mucosal immune response to an enteropathogen. we have used a chronically isolated intestinal-loop model in rabbits as a probe to monitor kinetically the initial (primary) local immune ...	1988	3278985
protein binding sites on escherichia coli 16s ribosomal rna; rna regions that are protected by proteins s7, s9 and s19, and by proteins s8, s15 and s17.	selected groups of isolated 14c-labelled proteins from e. coli 30s ribosomal subunits were reconstituted with 32p-labelled 16s rna, and the reconstituted complexes were partially digested with ribonuclease a. rna fragments protected by the proteins were separated by gel electrophoresis and subjected to sequence analysis. complexes containing proteins s7 and s19 protected an rna region comprising helices 29 to 32, part of helix 41, and helices 42 and 43 of the 16s rna secondary structure. additio ...	1988	3279390
toxoplasmosis and the acquired immune deficiency syndrome.	the literature on toxoplasmosis in the acquired immune deficiency syndrome is reviewed with reference to more than 140 cases from various centres. the incidence, pathogenesis, clinical features, diagnosis, treatment and prognosis are considered.	1988	3280690
quality of commercially produced shigella serogrouping and serotyping antisera.	shigella grouping antisera from five manufacturers and typing antisera from two were purchased and evaluated with homologous and heterologous shigella strains in the slide agglutination test. only 31 of 73 (42%) antisera were satisfactory. in many instances, the antisera gave negative, as opposed to weak, reactions when they should have given strong positive reactions. four reagents cross-reacted with shigella strains. of the 19 polyvalent grouping antisera to subgroups shigella dysenteriae sero ...	1988	3281972
cardiac nuclear medicine, present status, future hopes as seen from the view of a cardiologist.	the present techniques of nuclear medicine that are used in cardiology are critically evaluated. they are used mainly for the assessment of coronary circulation (measurement of regional flow and perfusion), on the determination of left ventricular muscle function (radionuclear ventriculography) and on the study of cardiac metabolism. the last-mentioned technique is unique insofar as (except for magnetic resonance imaging) there is no other method available to analyse myocardial metabolism noninv ...	1988	3282883
reaction of anti-hla-b monoclonal antibodies with envelope proteins of shigella species. evidence for molecular mimicry in the spondyloarthropathies.	immunologic cross-reactivity between enteric bacteria and the hla-b27 protein may play a role in the etiology of reiter's syndrome and reactive arthritis. the reactivity of two anti-b27 mab (b27m1 and b27m2) with envelope proteins of shigella flexneri isolated from reiter's syndrome patients was studied by western blot analysis. proteins with an apparent mr of approximately 36 and 23 kda reacted with both mab in ascites. mab against related hla class i ag b7 and b40 did not react with the 23 kda ...	1988	3283236
serum antibodies to lipopolysaccharide and natural immunity to shigellosis in an israeli military population.		1988	3283258
conservation of an atp-binding domain among reca proteins from proteus vulgaris, erwinia carotovora, shigella flexneri, and escherichia coli k-12 and b/r.	the purified reca proteins encoded by the cloned genes from proteus vulgaris, erwinia carotovora, shigella flexneri, and escherichia coli b/r were compared with the reca protein from e. coli k-12. each of the proteins hydrolyzed atp in the presence of single-stranded dna, and each was covalently modified with the photoaffinity atp analog 8-azidoadenosine 5'-triphosphate (8n3atp). two-dimensional tryptic maps of the four heterologous reca proteins demonstrated considerable structural conservation ...	1988	3286605
[clinico-immunological characteristics of mixed intestinal infections in children].		1988	3287323
[changes in the degree of sensitivity to antibiotics of shigella isolated from patients with dysentery in a city infectious disease hospital over the past 8 years].	changes in sensitivity of shigella and in particular shigella flexneri isolated from dysentery patients in a city infection hospital within 8 years i.e. from 1977 to 1985 were followed. it was shown that the changes were to the greater extent connected with preferable use of certain antibiotics in the hospital.	1988	3288150
suggestion to supplement shigella flexneri classification scheme with the subserovar shigella flexneri 4c: phenotypic characteristics of strains.	a new serovar of shigella flexneri has been isolated with increasing frequency in the ussr. it has the antigenic formula iv:7,8. we suggest that it be incorporated into the classification scheme as s. flexneri subserovar 4c. the 212 strains of this proposed subserovar examined to date display biochemical and serological properties typical of the species, are sensitive to the genus-specific bacteriophage, and cause keratoconjunctivitis in the sereny test.	1988	3290245
salmonellal mycotic abdominal-aortic aneurysm.	a patient is described with a mycotic abdominal-aortic aneurysm that was caused by salmonella typhimurium, who presented to hospital four months after a urinary-tract infection that was caused by the same organism. the urinary-tract infection may have resulted from an episode of transient salmonellal bacteraemia, the significance of which was not appreciated. the pathogenesis, clinical features, treatment and prognosis of arteritis due to salmonella species are reviewed. the early diagnosis and ...	1988	3292882
salmonellal mycotic abdominal-aortic aneurysm.	a patient is described with a mycotic abdominal-aortic aneurysm that was caused by salmonella typhimurium, who presented to hospital four months after a urinary-tract infection that was caused by the same organism. the urinary-tract infection may have resulted from an episode of transient salmonellal bacteraemia, the significance of which was not appreciated. the pathogenesis, clinical features, treatment and prognosis of arteritis due to salmonella species are reviewed. the early diagnosis and ...	1988	3292882
demetrio sodi-pallares: the man and his thought.		1988	3293862
[shigella serotypes and drug resistance patterns of strains isolated in etimesgut, turkey, in 1987].	drug resistance patterns of shigella strains were investigated in a prospective manner in etimesgut district during a period of 1 year. thirty strains shigella were isolated, belonged to three subgroups with preponderance of sh. flexneri (70%), followed by sh. sonnei (27%) and sh. boydii (3%). the resistance was highest with streptomycin (80%), followed by trimethoprim-sulfamethoxazole (tmp-smz) (53%) and ampicillin (43%). only three strains (10%) were sensitive to all eight antibiotics tested. ...	1988	3078794
death despite malaria prophylaxis.		1988	3133035
processing of neurofilament proteins from perikaryal to axonal type.	in previous studies, neuronal cell bodies, excised by hand from bovine spinal ganglia, were analyzed and heterogeneous intermediate and high molecular weight neurofilament proteins that differed in electrophoretic mobility from their axonal counterparts were demonstrated. in the present experiment, intermediate and high molecular weight neurofilament proteins of the axonal type were treated with alkaline phosphatase, and neurofilament proteins enriched in perikaryal type proteins were labeled wi ...	1988	3137483
[dysenteric arthritis--a case-related observation].		1988	3140351
expression of four virulence antigens of shigella flexneri is positively regulated at the transcriptional level by the 30 kilodalton virf protein.	on the virulence plasmid of shigella flexneri the virg region required for cell-to-cell spread of the bacterium encodes a 130 kilodalton (kd) antigen and region-2 essential for the bacterial invasion of epithelial cells encodes 57, 43 and 39 kd antigens. the expression of these four antigens is positively regulated by the 30 kd protein encoded by virf, whose nucleotide sequence had been determined and which was previously found to be essential for virulence. an approximately 3.8 kilobase (kb) rn ...	1988	3141742
short- and long-term effects of a single dose of bisphosphonates on retinoid-induced bone resorption in thyroparathyroidectomized rats.	the inhibitory effect of a single subcutaneous (s.c.) dose of three different bisphosphonates (bps)--4-amino-1-hydroxybutylidene-1,1-bisphosphonate (ahbubp), 2-(2-pyridinyl)-ethylidene-1,1-bisphosphonate (2-pebp), and dichloromethylene-bisphosphonate (cl2mbp)--was studied in a model of retinoid-induced bone resorption, which consists of assessing the hypercalcemic effect of the arotinoid ro 13-6298 given s.c. for three days in thyroparathyroidectomized (tptx) rats. the retinoid was given on day ...	1988	3145796
plasmid dna survey of clinically isolated shigella strains in shanghai area.		1988	3145835
effects of maternal exposure to polychlorobiphenyls (pcbs) on f1 generation behavior in the rat.	the effect of fenclor 42 (pcb) exposure of female rats (fischer 344 strain) was studied through assessment of the behavioral development of their f1 progeny. female rats were exposed to pcb according to the following treatment schedule: (a) (5 days) 2 weeks prior to mating, (b) during gestation (days 6-15 of pregnancy), (c) during lactation (days 1-21 after delivery). behavioral endpoints of motor reflexes, motor coordination, activity (preweaning behaviors), and learning (postweaning behavior) ...	1988	3146520
scopolamine produces environment-specific conditioned activity that is not blocked by pimozide in rats.	a classical conditioning paradigm was employed to determine if the stimulant effects of the anticholinergic scopolamine could show conditioning. in experiment 1 rats had 12 60-min pairings of scopolamine (1.0 or 8.0 mg/kg, ip) with a distinctive environment that monitored horizontal and vertical activity. experimental (paired) groups received the drug 30 min prior to each session, whereas control (unpaired) groups received saline. following each session the paired groups were injected with salin ...	1988	3146771
effects of interferon alpha on performance in man: a preliminary report.	the effects of three doses of interferon alpha (1.5 mu, 0.5 mu and 0.1 mu) on performance were studied. the injection of 1.5 mu ifn produced symptoms and performance changes which closely resembled those found in volunteers with influenza. specifically, volunteers were slower at responding when they were uncertain when a target stimulus would appear, but were not impaired on a pursuit tracking task or syntactic reasoning task. the results suggest that interferon-induced changes in cns function p ...	1988	3146777
[the effect of long-term exposure to gamma rays (6,7 gy) on the activity of monoamine oxidase in the hypothalamus of sheep during the anestrus period].		1988	3146833
the influence of duration of photoperiod and hemicastration on growth and testicular and endocrine functions of boars.	yorkshire boars were used to evaluate the influence of duration of photoperiod and hemicastration on growth and testicular and endocrine functions. at 10 wk of age, 5 hemicastrate (hc) and 5 intact (i) boars were assigned to either 8 or 16 hr of light daily until 6 mo of age. body weights were recorded biweekly throughout the experiment. venous cannulae were placed in all boars at 6 mo of age, and serum was collected at 30 min intervals from 0800 to 2000 hr. gonadotropin releasing hormone (gnrh) ...	1988	3147165
carbonic anhydrase isoenzymes in the caecum and colon of normal and germ-free rats.	histochemical and immunocytochemical methods were used to study the presence of carbonic anhydrase (ca) isoenzymes in the caecum and colon of normal and germ-free rats. very high enzyme activity was demonstrated by histochemistry in the caecum and proximal colon of normal rats, while the activity decreased in the distal colon. very strong immunostaining for the isoenzyme ca i was found in the cytoplasm of surface cells and upper gland cells in the caecum and colon of normal rats. in the distal c ...	1988	3147569
in vivo measurement of intracellular ph in human brain during different tensions of carbon dioxide in arterial blood. a 31p-nmr study.	the effect of changes in carbon dioxide tension in arterial blood upon intracellular ph in brain tissue was studied in seven healthy volunteers, aged 22-45 years. the ph changes were monitored by use of 31p nuclear magnetic resonance spectroscopy, performed on a whole-body 1.5 tesla siemens imaging system. the measurements were carried out during hyperventilation and with the subject breathing atmospheric air containing 5 vol. % and 7 vol. % carbon dioxide. intracellular ph increased significant ...	1988	3147578
the development of immunological relationship between mother and fetus under physiological and pathological conditions.	on the basis of results of our research and review of literature, the complex of immuno logical influences, operating during the development of the human fetus, were evaluated. it is obvious that during the early stages of pregnancy the conceptus is protected by non-specific mechanisms, i.e. hormonally (hcg, progesterone) and by certain properties of the trophoblast (barrier function, immunologically inert surface). specific immunological tolerance is formed by gradual penetration of trophoblast ...	1988	3147589
comparison of the invasion strategies used by salmonella cholerae-suis, shigella flexneri and yersinia enterocolitica to enter cultured animal cells: endosome acidification is not required for bacterial invasion or intracellular replication.	strains of escherichia, salmonella, shigella and yersinia actively enter eukaryotic cells. several techniques were used to compare and contrast the invasion mechanisms of salmonella cholerae-suis, yersinia enterocolitica and shigella flexneri. three animal cell lines (cho, hep-2 and mdck) were examined for susceptibility to bacterial entry by these strains. levels of intracellular bacteria varied widely between cell lines, but cho cells were the most susceptible to bacterial invasion, hep-2 inva ...	1988	3147700
[lysosomal enzyme activity in the nerve tissue of various species of animals after administration of a neurotoxic pesticide aphos].		1988	3149613
production of prostaglandins and hete-compounds by isolated-cultivated bovine aortic endothelial cells.	the main product of aa conversion by baec is pgi2. other pgs were synthesized by confluent cells only in traces with exception of a hht-like compound which has still to be characterized in more details. a significant release of hete was only detected when baec were destroyed. under these conditions about 1 pmole/10(5) cells 15- and 12 hete were released by baec. taken into account the amounts of hete produced by endothelial cells as well as the partially contradictory activities of 12- and 15-he ...	1988	3150276
anti-cancer therapy with valine-depleted amino acid imbalance solution.	for the purpose of developing amino acid imbalance solution applicable to cancer treatment, we prepared seven kinds of amino acid imbalance solutions based on a 10% balanced amino acid solution and investigated the anti-tumor effects of each solution. the administration of valine-depleted amino acid solution for 8 days at a daily dose of 53 ml/rat (79.5 kcal/rat) resulted in the most significant inhibitory effects on the growth of hepatoma (ah109a) and mammary tumors (mrmt-1), the rate being 82. ...	1988	3150873
urinary infection and staghorn calculus in the left kidney.		1988	3153046
effects of acetyl strophanthidin on duration of atrial fibrillation in the neurally-intact and blockaded dog.	although the inotropic and dromotropic effects of cardiac glycosides in atrial fibrillation (af) are well recognized, their action on af itself is not clear. accordingly, to determine whether cardiac glycosides prolong af, the duration of electrically induced af, atrioventricular conduction, and left ventricular function were assessed for 30 minutes before and for 30 minutes following intravenous administration of acetyl strophanthidin (as), 20 micrograms/kg, in neurally intact, beta-blocked, an ...	1988	3154634
estimation of pyruvate dehydrogenase (e1) activity in human skeletal muscle; three cases with e1 deficiency.	pyruvate dehydrogenase (e1) catalyzes the rate-limiting step of the pyruvate dehydrogenase complex. since e1 activity of human muscle tissue is low, a sensitive method is needed for diagnostic purposes. measurement of 14co2 production from [1-14c]pyruvate provides a specific and sensitive assay for measuring e1 activity. we use as artificial electron acceptor dichlorophenolindophenol (dcpip) instead of the often applied ferricyanide. the method can be applied to small muscle samples obtained by ...	1988	3127087
feline aortic thromboemboli.		1988	3128904
inhibition of beta-bungarotoxin binding to brain membranes by mast cell degranulating peptide, toxin i, and ethylene glycol bis (beta-aminoethyl ether)-n,n,n',n'-tetraacetic acid.	the presynaptically active snake venom neurotoxin beta-bungarotoxin (beta-butx) is known to affect neurotransmitter release by binding to a subtype of voltage-activated k+ channels. here we show that mast cell degranulating (mcd) peptide from bee venom inhibits the binding of 125i-labeled beta-butx to chick and rat brain membranes with apparent ki values of 180 nm and 1100 nm, respectively. the mechanism of inhibition by mcd peptide is noncompetitive, as is inhibition of 125i-beta-butx binding b ...	1988	3130091
activities of some enzymes of phospholipid metabolism in cultured rat ventricular myocytes in normoxic and hypoxic conditions.	phospholipid catabolism is thought to be one of the critical events in membrane injury during heart ischemia. in this work, the enzymes involved in phospholipid metabolism were studied in purified cultured ventricular myocytes in normoxic and hypoxic conditions. purified ventricular myocytes exhibited an alkaline phospholipase a activity which had sn-2 specificity and which was calcium dependent, and an acid phospholipase a activity with sn-1 specificity. these cells also exhibited lysophospholi ...	1988	3334866
metaplastic breast carcinoma with epithelial phenotype of pseudosarcomatous components.	a case of metaplastic breast carcinoma was subjected to an immunohistochemical study to characterize its apparent fibrosarcomatous and chondrosarcomatous elements. polyclonal and monoclonal antibodies against epithelial cell components such as keratin proteins, epithelial membrane antigen, membrane and cytoplasmic antigens of human mammary carcinoma cells, and carcinoembryonic antigen were used, as well as antibodies against the mesenchymal antigens, desmin, and vimentin. the cells with a mesenc ...	1988	3337621
regulation of central cholecystokinin recognition sites by guanyl nucleotides.	guanyl nucleotides affected the binding of radiolabeled cholecystokinin (cck) octapeptide to rodent cortical binding sites. micromolar quantities of a stable gtp analogue, guanylyl-imidodiphosphate (gppnp), resulted in a plateau where binding was decreased by 30%. in the presence of 25 microm gppnp, binding analysis revealed a decrease in affinity (increase in kd), without an apparent effect on the maximal number of binding sites. ki values for cck-related peptides shifted up to 1.6-fold. the ra ...	1988	3339367
anterior perineal dislocation of the hip with fracture of the femoral head. a case report.	traumatic anterior perineal hip dislocation with an associated fracture of the femoral head is a rare entity. a 26-year-old man injured in a motorcycle accident was treated by closed reduction of the dislocation within three hours after admission. however, several reports of patients with anterior hip dislocation with associated femoral head fractures were treated nonoperatively and had unfavorable results when treatment failed to achieve anatomical position of the fragments. consequently, this ...	1988	3342566
lactic acidosis and recovery of neuronal function following cerebral hypoxia in vitro.	the rat hippocampal slice preparation was used to study the combined effects of hypoxia and lactic acidosis on neuronal function. control slices were exposed to a standard hypoxic insult while being perfused with normal artificial cerebrospinal fluid (acsf). experimental slices were perfused with acsf containing 1.0, 2.0, 10.0 or 20.0 mm lactic acid, 30 min before and during the same standard hypoxic insult. following at 30-min recovery period the ability of these slices to respond to orthodromi ...	1988	3345436
chemosensory anterior dorsal fin in rocklings (gaidropsarus and ciliata, teleostei, gadidae): somatotopic representation of the ramus recurrens facialis as revealed by transganglionic transport of hrp.	the anterior dorsal fin in rocklings consists of a fringe of 50-80 delicate, vibratile rays, which are densely beset with epidermal chemosensory cells. the innervation of these cells is from the dorsal branch of the recurrent facial nerve, which also innervates all other fins and the skin of the trunk. this nerve carries at least three classes of fibres: small (0.5-1.5 micron in diameter), medium (1.5-4 micron), and large (greater than 4 micron). approximately 12,000 small and weakly myelinated ...	1988	3346379
differences in synaptic inputs to preganglionic neurons in the dorsal and lateral band subdivisions of the cat sacral parasympathetic nucleus.	in the cat, preganglionic neurons (pgns) found in the dorsal portion of the sacral parasympathetic nucleus (dorsal band or db cells) participate in bowel control, while those found along the lateral edge (lateral band or lb cells) influence bladder function. in order to determine whether differences in the synaptic inputs exist between these two populations, hrp was applied to the sacral ventral rootlets of cats, and the s2 cord segment was prepared for sequential light and electron microscopy. ...	1988	3346386
corticotropin-induced changes in enzymatic activities of the post-pregnenolone steroidogenic pathway in rabbit adrenocortical cells.	in an attempt to delineate the effect of corticotropin (acth) on post-pregnenolone steroidogenesis, the activity of enzymatic systems operative in conversion of pregnenolone into glucocorticoids and androgens was studied in adrenocortical cells from control rabbits and from animals treated with acth for 12 days (acth 1-24, 200 micrograms s.c. daily). the cells from acth-treated rabbits exhibited an increased overall steroidogenic capacity and produced much more cortisol (p less than 0.0005) as w ...	1988	3347065
[meningoencephalitis and mononucleosis syndrome associated with acute infection by human immunodeficiency virus].		1988	3347109
functionally illiterate worker also has 'right to understand'.		1988	3347451
the duck hepatitis b virus dna polymerase is tightly associated with the viral core structure and unable to switch to an exogenous template.	the duck hepatitis b virus (dhbv) has a dna polymerase associated with it which uses the incomplete viral genome as endogenous template. a prerequisite for studying this polymerase is the availability of conditions to open viral cores without destroying their enzymatic activity. in this study, this was achieved by a brief treatment with low ph. dhbv dna in low-ph-treated cores was susceptible to digestion with deoxyribonuclease i and restriction enzymes, and large restriction fragments diffused ...	1988	3347995
susceptibilities of hong kong isolates of multiply resistant shigella spp. to 25 antimicrobial agents, including ampicillin plus sulbactam and new 4-quinolones.	seventy-two percent of 129 shigella isolates from two hong kong hospitals were shigella flexneri. twenty-six percent were s. sonnei, and there was only one isolate each of s. dysenteriae and s. boydii. ninety-six percent of the isolates were resistant to two or more antibiotics, and up to 11 resistances were seen in a single isolate. fifty-seven percent or more of these isolates were resistant to ampicillin, streptomycin, tetracycline, chloramphenicol, and sulfamethoxazole; and up to twenty-thre ...	1988	3348608
[amino acid composition of the lymph and blood in fever reactions of different durations].	the amino acid composition of the thoracic duct lymph and blood following fever reactions (fr) of various duration has been studied in the experiments on rabbits. the experiments have shown that some changes in protein metabolism in fr are primarily manifested in amino acid lymph composition. irrespective of fr duration the amino acid content in the lymph almost always increases, which confirms the opinion concerning catabolic process predominance in protein metabolism during fr. the lack of "cu ...	1988	3349141
anti-inflammatory activity of oleoresin from brazilian copaifera.	the oleoresin from brazilian copaifera species yielded copalic acid and sesquiterpenes and showed marked anti-inflammatory activity using various experimental models in rats. the oleoresin significantly inhibited carrageenin-induced pedal edema following oral doses from 0.70 to 2.69 ml/kg, but was somewhat less effective than 50 mg/kg calcium phenylbutazone. repeated administration of the oleoresin at a dose of 1.26 ml/kg for a 6-day period reduced granuloma formation with a response comparable ...	1988	3352280
[tobacco or health: choose health].		1988	3352398
chorioamnionitis: a study of organisms isolated in perinatal autopsies.	the purpose of this study was to determine the validity of postmortem cultures in perinatal autopsies; in particular, those cases where there is gross or histologic evidence of chorioamnionitis. lung, liver, blood, and placental cultures were obtained from 159 neonatal autopsies with histologic evidence of chorioamnionitis at magee-women's hospital between january 1980 and july 1985. the criterion for chorioamnionitis was a polymorphonuclear leukocytic infiltrate in the placental membranes. the ...	1988	3355095
septal thickness in human lungs assessed by computerized interactive morphometry.	we have studied the feasibility of using interactive computerized morphometric methods for the quantitation of septal thickness in the lung with the goal of introducing objective criteria for the diagnosis and grading of pulmonary fibrosis and edema. after reviewing available methods, we have decided in favor of linear intercept measurements of septal profiles which are limited to the tissue and therefore not affected by the level of lung inflation. our method consisted in generating a graphic o ...	1988	3357335
responsiveness of genetically epilepsy-prone rats to intracerebroventricular morphine-induced convulsions.	the sensitivity to intracerebroventricular morphine-induced convulsions was determined in members of the severe seizure (gepr-9) and moderate seizure (gepr-3) colonies of genetically epilepsy-prone rats as well as in non-epileptic control rats. gepr-9s were more sensitive to morphine-induced wet-dog shakes, rearing with bilateral forelimb clonus and generalized clonus than controls of gepr-3s. gepr-3s were less sensitive to morphine-induced wet-dog shakes and rearing with bilateral forelimb clon ...	1988	3362037
effects of lesions of the dorsal noradrenergic bundle on successive discrimination in the rat.	male sprague-dawley rats were trained to bar-press for food reward on a successive discrimination involving periods of reward on a variable-interval (vi) 18-s schedule interspersed with periods of extinction. the two components of the schedule were signaled by a steady or a flashing light, counterbalanced between vi and extinction components. confirming previous findings, the discrimination was easier when the flashing light signaled vi and the steady light signaled extinction, than with the rev ...	1988	3365184
n-acetylcysteine inhibits human neutrophil and monocyte chemotaxis and oxidative metabolism.	the effect of n-acetylcysteine (nac) on human neutrophil and monocyte cell viability, chemotaxis, oxygen consumption and chemiluminescence was studied. it was found that nac at concentrations higher than 3 x 10(-2) m resulted in neutrophil and monocyte cytotoxicity. the studies on the effect of nac on neutrophil and monocyte chemotaxis showed that nac inhibited chemotaxis of both cell types in a concentration dependent manner. nac at 3 x 10(-2) m inhibited chemotaxis of both cell types by about ...	1988	3366508
a comparison of the effects of measured, predicted, estimated and constant residual volumes on the body density of female athletes.	the body density (bd), and hence the relative body fat (% bf) was measured for 182 female athletes. the residual volume (rv) was determined both before and after the underwater weighing by a multiple breath helium dilution technique with the subject immersed to neck level. the absolute mean difference (lxdl) and see between the two rv trials were 63 and 75 ml, respectively. these increased to values ranging 144-685 and 187-252 ml, respectively, when the mean of the two rv trials for each subject ...	1988	3366515
antimicrobial susceptibility testing of streptococcus pneumoniae: quality assessment results.	six strains of streptococcus pneumoniae were distributed to 405 united kingdom laboratories who were asked to test the susceptibility of the strains to penicillin, tetracycline, chloramphenicol and erythromycin and to provide details of methodology to test the standards of susceptibility testing. high error rates were seen only in failure to detect moderate resistance to penicillin (12%) and resistance to chloramphenicol (16%). increased error rates were associated with several methods or practi ...	1988	3366921
[effect of prolactin on the enzymatic activity of thyroid follicular cells during stress due to surgery and subsequent subcutaneous inflammation].	in order to reveal prolactin (prl) effect on the enzymatic activity of the thyroid follicular cells at a subcutaneous inflammation, 2 series of experiments have been performed on 100 non-inbred male rats with body mass of 150-170 g. in order to produce the inflammation, under ether narcosis celloidin globules are introduced subcutaneously in the abdominal wall. the test animals are given 0.125 mg of "lactin" (the soviet preparation of bovine prl) in 0.5 ml of isotonic solution once every day, be ...	1988	3369954
[effect of caldesmon on the type of conformation changes in f-actin induced by the binding of myosin 1 subfragment].	the effect of caldesmon on the conformational changes of f-actin caused by myosin subfragment 1 (s-1) binding was studied, using the polarized microfluorimetry method. it was demonstrated that the polarized fluorescence of rhodaminil-phalloin specifically bound to f-actin of pure actin filaments as well as of tropomyosin-containing actin filaments changes as a result of binding to s-1. the nature of these changes depends on the presence of caldesmon in the filaments. caldesmon was supposed to mo ...	1988	3370245
increased hepatic amino nitrogen conversion after elective cholecystectomy in man.	1. the effect of elective, uncomplicated cholecystectomy on plasma clearances of amino acids and on amino acid-stimulated urea synthesis was investigated in 10 patients, pre-operatively and on the first post-operative day, and compared with six controls treated identically apart from the surgery. 2. a mixture of amino acids was given as a prime-continuous infusion. steady-state concentrations 75% higher than basal were attained and were maintained for 90 min. the clearances of amino acids were c ...	1988	3370921