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sigmab activation under environmental and energy stress conditions in listeria monocytogenes.to measure sigmab activation in listeria monocytogenes under environmental or energy stress conditions, quantitative reverse transcriptase pcr (taqman) was used to determine the levels of transcripts for the sigmab -dependent opuca and clpc genes in strains having null mutations in genes encoding regulator of sigma b proteins (rsbt and rsbv) and sigma b (sigb) and in the l. monocytogenes wild-type 10403s strain under different stress conditions. the deltasigb, deltarsbt, and deltarsbv strains pr ...200616885265
exposure to salt and organic acids increases the ability of listeria monocytogenes to invade caco-2 cells but decreases its ability to survive gastric stress.the effects of environmental stress exposure on listeria monocytogenes growth and virulence-associated characteristics were investigated. specifically, we measured the effects of temperature (7 or 37 degrees c), ph (5.5 or 7.4), the presence of salt and organic acids (375 mm nacl, 8.45 mm sodium diacetate [sd], 275 mm sodium lactate [sl], or a combination of nacl, sd, and sl), and deletion of sigb, which encodes a key stress response regulator, on the ability of l. monocytogenes to grow, invade ...200616885290
a mutation in the luxs gene influences listeria monocytogenes biofilm formation.using a vibrio harveyi reporter strain, we demonstrated that listeria monocytogenes secretes a functional autoinducer 2 (ai-2)-like signal. a luxs-deficient mutant produced a denser biofilm and attached to a glass surface 19-fold better than the parent strain. exogenous ai-2 failed to restore the wild-type phenotype to the mutant. it seems that an intact luxs gene is associated with repression of components required for attachment and biofilm formation.200616885324
vaccination strategy determines the emergence and dominance of cd8+ t-cell epitopes in a fvb/n rat her-2/neu mouse model of breast cancer.the her-2/neu oncogene has >25 hla epitopes, yet only one fvb/n mouse cd8(+) t-cell epitope has been mapped to date. this epitope has been termed the immunodominant epitope for the fvb/n mouse, but we propose that the vaccination strategy determines the dominance of epitopes. using a series of overlapping peptides, we have mapped another cd8(+) t-cell epitope that emerges in the fvb/n mouse following vaccination with listeria monocytogenes-based vaccines that express fragments of her-2/neu. foll ...200616885378
delayed expansion and contraction of cd8+ t cell response during infection with virulent salmonella typhimurium.ag presentation to cd8(+) t cells often commences immediately after infection, which facilitates their rapid expansion and control of infection. subsequently, the primed cells undergo rapid contraction. we report that this paradigm is not followed during infection with virulent salmonella enterica, serovar typhimurium (st), an intracellular bacterium that replicates within phagosomes of infected cells. although susceptible mice die rapidly (approximately 7 days), resistant mice (129 x 1svj) harb ...200616849458
visualization of il-12/23p40 in vivo reveals immunostimulatory dendritic cell migrants that promote th1 differentiation.il-12p40 is induced in macrophages and dendritic cells (dc) after activation by microbial tlr ligands and cytokines and constitutes a component of il-12 and il-23. in an effort to understand the location and kinetics of these cytokines during the course of an immune response, we generated knockin (gene-targeted) mice that express the p40 gene linked via a viral internal ribosome entry site element with fluorescent reporters, eyfp or egfp. macrophages and dc from these mice faithfully reported bi ...200616849470
innate inflammatory signals induced by various pathogens differentially dictate the ifn-i dependence of cd8 t cells for clonal expansion and memory formation.type-i ifns (ifn-i) provide direct survival signals to t cells during ag-driven proliferation. because ifn-i production differs depending on the pathogen, we assessed cd8 t cell requirement for direct ifn-i signals during responses to vaccinia virus (vv), vesicular stomatitis virus (vsv), lymphocytic choriomeningitis virus (lcmv), and listeria monocytogenes (lm) immunizations in vivo. ifn-i-receptor-deficient (ifn-ir(o)) cd8 t cells expanded 3- to 5-fold less and formed a diminished memory pool ...200616849484
the adenosine system selectively inhibits tlr-mediated tnf-alpha production in the human newborn.human newborns are susceptible to microbial infection and mount poor vaccine responses, yet the mechanisms underlying their susceptibility are incompletely defined. we have previously reported that despite normal basal expression of tlrs and associated signaling intermediates, human neonatal cord blood monocytes demonstrate severe impairment in tnf-alpha production in response to triacylated (tlr 2/1) and diacylated (tlr 2/6) bacterial lipopeptides (blps). we now demonstrate that in marked contr ...200616849509
modelling the individual cell lag time distributions of listeria monocytogenes as a function of the physiological state and the growth conditions.the individual cell lag time distributions of listeria monocytogenes were characterized for 54 combinations of 22 initial physiological states, 18 growth conditions, and 11 strains. the individual cell lag times were deduced from the times for cultures issued from individual cells to reach an optical density threshold. the extreme value type ii distribution with a shape parameter set to 5 was shown effective to describe the 54 observed distributions. the theoretical distributions of individual l ...200616857284
enhancement of mice susceptibility to infection with listeria monocytogenes by the treatment of morphine.the effect of morphine on the susceptibility of balb/c mice to diarrheagenic escherichia coli, shigella flexneri, listeria monocytogenes, salmonella enteritidis, yersinia enterocolitica, was examined via the intraperitoneal inoculation. morphine treatment increased the susceptibility to s. enteritidis and l. monocytogenes, resulting in bacteremia and central nervous system (cns) invasion (for l. monocytogenes), while the infection with other bacteria did not show the systemic dissemination in th ...200616858145
regulated translation of listeriolysin o controls virulence of listeria monocytogenes.listeria monocytogenes is an intracytosolic bacterial pathogen that escapes from the phagosome using a secreted cytolysin, listeriolysin o (llo). in the host cytosol, llo activity is minimized to prevent pore formation in the host plasma membrane; premature lysis of the infected host cell exposes the bacteria to extracellular immune defences of the host and is detrimental to infection. here we identified nucleotide substitutions in the coding sequence of the llo gene (hly) that did not alter the ...200616859495
vaccination with irradiated listeria induces protective t cell immunity.we evaluated gamma-irradiated listeria monocytogenes as a killed bacterial vaccine, testing the hypothesis that irradiation preserves antigenic and adjuvant structures destroyed by traditional heat or chemical inactivation. irradiated listeria monocytogenes (lm), unlike heat-killed lm, efficiently activated dendritic cells via toll-like receptors and induced protective t cell responses in mice. like live lm, irradiated lm induced toll-like-receptor-independent t cell priming. cross-presentation ...200616860763
isolation of listeria monocytogenes from buffaloes with reproductive disorders and its confirmation by polymerase chain reaction.listeria monocytogenes, a gram-positive, facultative intracellular pathogen was isolated from buffaloes with a history of reproductive disorders and polymerase chain reaction (pcr) analyses for the presence of virulence-associated genes were conducted. a total of 530 samples of faecal, nasal, vaginal swabs and blood samples from 135 buffaloes were screened. the prevalence of l. monocytogenes and other listeria spp. was found to be 4.4 and 7.4%, respectively. all isolates were subjected to pcr fo ...200616860946
the role of macrophage migration inhibitory factor in lethal listeria monocytogenes infection in mice.macrophage migration inhibitory factor (mif) has been characterized as a proinflammatory cytokine. previous studies have indicated that mif may play a beneficial role or a detrimental role in microbial infections, depending on pathogens. in this study, we investigated the role of mif in listeria monocytogenes infection. the mif titers increased 6h after lethal l. monocytogenes infection but not in the sublethal infection. the elimination of bacteria from the spleens and livers was not affected b ...200616863687
prevalence of listeria monocytogenes and other listeria species in butter from united kingdom production, retail, and catering premises.two recent listeriosis outbreaks involving butter prompted this first cross-sectional study on the prevalence, levels, and types of listeria species in 3229 samples of butter from production, retail, and catering premises in the united kingdom during may and june 2004. when the criteria of the microbiological guidelines were used, 99.4% of samples were found to be of satisfactory microbiological quality, 0.5% were of acceptable quality, and 0.1% were of unsatisfactory quality as a result of high ...200616865880
attachment of listeria monocytogenes to an austenitic stainless steel after welding and accelerated corrosion treatments.austenitic stainless steels, widely used in food processing, undergo microstructural changes during welding, resulting in three distinctive zones: weld metal, heat-affected zone, and base metal. this research was conducted to determine the attachment of listeria monocytogenes in these three zones before and after exposure to a corrosive environment. all experiments were done with tungsten inert gas welding of type 304 stainless steel. the four welding treatments were large or small beads with hi ...200616865881
survival of listeria monocytogenes in uncooked italian dry sausage (salami).this study was undertaken to supplement existing information on the survival of listeria monocytogenes in italian salami. the fact that italian salami is frequently consumed by a large number of people poses some serious health implications. some raw materials have been found to be microbiologically contaminated, for their production occurs without any thermic treatment, and these are in circulation throughout italy all year round. we selected the product for its microbiological, technological, ...200616865882
a predictive model to describe the effects of temperature, sodium lactate, and sodium diacetate on the inactivation of a serotype 4b strain of listeria monocytogenes in a frankfurter slurry.a modified gompertz equation was used to model the effects of temperature (55, 60, and 65 degrees c), sodium lactate (0, 2.4, and 4.8%), and sodium diacetate (0, 0.125, and 0.25%) on inactivation of listeria monocytogenes strain mfs 102 (serotype 4b) in frankfurter slurry. the effects of these factors were determined on the shouldering region (parameter a), maximum death rate (parameter b), and tailing region (parameter c) of microbial inactivation curves. increased temperature or sodium diaceta ...200616865885
effect of x-ray irradiation on reducing the risk of listeriosis in ready-to-eat vacuum-packaged smoked mullet.listeria monocytogenes can pose a serious threat in several areas of the nation's food supply including ready-to-eat seafood products. use of irradiation processing can potentially reduce the risk of listeriosis caused by consumption of ready-to-eat seafood products. this study measured the effect of x-ray irradiation on reducing the population of l. monocytogenes on ready-to-eat, vacuum-packaged smoked mullet. smoked mullet were inoculated with a five-strain mixture of l. monocytogenes (10(4) c ...200616865886
efficacy of disinfectants to reduce listeria monocytogenes on precut iceberg lettuce.the efficacy of water, chlorinated water (100 ppm), peracetic acid solution (0.05%), and commercial citric acid-based produce wash (0.25%) to reduce the population of listeria monocytogenes on precut lettuce was tested. samples were inoculated with a mixture of equal amounts of five l. monocytogenes strains at a level of 4.7 log cfu/g, and analyzed on the day of washing and after 3 and 6 days of storage at 6 degrees c. sanitizer reduced the number of l. monocytogenes at maximum 1.7 log cfu/g and ...200616865887
adhesion properties and competitive pathogen exclusion ability of bifidobacteria with acquired acid resistance.the adhesion properties of bifidobacterium longum and bifidobacterium catenulatum strains with an acquired resistance to acid and their ability to competitively exclude salmonella enterica serovar typhimurium, escherichia coli, listeria monocytogenes, enterobacter sakazakii, and clostridium difficile from adhering to human intestinal mucus were evaluated and compared with the results when the same experiments were run with the original acid-sensitive strains. in half of the four studied cases, t ...200616865903
molecular epidemiology and cluster analysis of human listeriosis cases in three u.s. states.to better understand the transmission and epidemiology of human listeriosis, 647 listeria monocytogenes isolates obtained from human listeriosis cases in four u.s. locations (michigan, ohio, new york state, and new york city) over 61 months (1998 to 2003) were characterized by automated ecori ribotyping. a total of 65 ribotypes were differentiated among the characterized isolates; 393, 227, and 24 isolates were classified into lineages i, ii, and iii, respectively, and 3 isolates were not classi ...200616865904
antimicrobial activity of extracts of the lichen xanthoparmelia pokornyi and its gyrophoric and stenosporic acid constituents.the antimicrobial activity of the diethyl ether, acetone, chloroform, petroleum ether, and ethanol extracts of the lichen xanthoparmelia pokornyi and its gyrophoric acid and stenosporic acid constituents has been screened against some foodborne bacteria and fungi. both the extracts and the acids showed antimicrobial activity against aeromonas hydrophila, bacillus cereus, bacillus subtilis, listeria monocytogenes, proteus vulgaris, staphylococcus aureus, streptococcus faecalis, yersinia enterocol ...200616869486
survival of listeria monocytogenes and enterococcus faecium in sludge evaluated by real-time pcr and culture methods.this study evaluates the behaviour in spiked sludge of a pathogenic bacteria, listeria monocytogenes, by cultural and molecular techniques, and compares its survival with the one of a faecal indicator, enterococcus faecium.200616869894
effects of acidified sodium chlorite, cetylpyridinium chloride and hot water on populations of listeria monocytogenes and staphylococcus aureus on beef.the present study was designed to determine the individual and combined effects of acidified sodium chlorite (asc, 0.1%, 24 +/- 1 degrees c), cetylpyridinium chloride (cpc, 0.5%, 24 +/- 1 degrees c) and hot water (hw, 93 +/- 1 degrees c) treatments on the survival of listeria monocytogenes and staphylococcus aureus.200616869900
contribution of penicillin-binding protein homologs to antibiotic resistance, cell morphology, and virulence of listeria monocytogenes egde.seven open reading frames, annotated as potential penicillin-binding-protein-encoding genes (lmo0441, lmo0540, lmo1438, lmo1892, lmo2039, lmo2229, and lmo2754), were targeted for insertional mutagenesis in listeria monocytogenes egde. these genes were found to contribute in various degrees to beta-lactam resistance, cell morphology, or the virulence potential of this organism.200616870778
safety of etanercept in psoriasis: a critical review.conventional systemic treatments for patients with psoriasis are associated with multiple adverse effects that require continuous monitoring. the introduction of new biological agents such as etanercept, a fully human fusion protein, has permitted individualisation of patients' treatment according to disease stage. the drug is a competitive inhibitor of tumour necrosis factor-alpha (tnfalpha) that prevents interaction between this cytokine and its cell surface receptors. etanercept also modulate ...200616872241
acute bacterial meningitis in qatar.to study the changes in the epidemiology, clinical and bacteriological profiles of bacterial meningitis in the era of the haemophilus influenzae type b (hib)vaccine and pneumococcus resistance.200616501676
[serovars of listeria monocytogenes and related species isolated from human clinical specimens].using phenotype techniques, characterization was realized of species and serovars of 255 strains of listeria isolated from human material: 220-86.3% were from patients with possible invasive disease, while 35-13.7% were from colonized healthy individuals. the strains were collected in several regions of brazil from 1969-2000. in individuals aged 0-10 or 41-60 years old listeria monocytogenes was isolated more often in cerebral spinal fluid than in blood cultures, including samples from renal tra ...200616501763
microbiological quality of randomly selected ready-to-eat foods sampled between 2003 and 2005 in wales, uk.since 1995, the publicly funded ready-to-eat food sampling and examination activities in wales have been coordinated and structured, using a novel approach for the identification of samples and premises. the latest set of data from this surveillance system reports the results from 3391 ready-to-eat foods sampled between november 2003 and march 2005. during this seventeen-month period all samples were examined for aerobic colony count, escherichia coli, listeria spp., bacillus cereus, salmonella, ...200616503065
lentinan has a stimulatory effect on innate and adaptive immunity against murine listeria monocytogenes infection.lentinan, a (1-3)-beta glucan from lentinus edodes, is licensed as an immunostimulatory drug. we tested the effect of lentinan in the well-established model system of the murine listeria monocytogenes infection. pre-treatment of bone marrow macrophages and dendritic cells with lentinan resulted in increased production of tnf-alpha and il-12 after l. monocytogenes infection in vitro. after lentinan treatment bone marrow macrophages showed increased no-production and enhanced cytotoxic activity ag ...200616504933
[zoonotic importance of selected species of gram-positive bacteria.]zoonoses continue to play a signicant role in both human and veterinary medicine. their eradication is difficult and some authors are convinced of their growing importance. among the pathogens of infectious diseases transmissible from animals to man are several gram-positive bacteria. the authors focus on three agents that present a risk in three different areas-alimentary infections (listeria monocytogenes), professional infections (streptococcus suis) and infections of breeders of so-called pe ...200616508905
infection with listeria monocytogenes as a probe for placental immunological function.this chapter will describe the use of infection with listeria monocytogenes, a gram-positive intracellular bacterium, to study immunological responses in the placenta. this bacterium is chosen because it has a predilection for replication in the placenta. as such, it is a significant pathogen for pregnant women, being a major cause of fetal mortality and morbidity if appropriate public health precautions are not observed. furthermore, this bacterium has been a major tool for studying innate immu ...200616512000
improved dna probe detection of listeria monocytogenes in enrichment culture after physical-chemical fractionation.bacterial detection in foods by nucleic acid probes is limited by microflora competition during selective enrichment. probe target concentration by extraction and fractionation of enrichments may diminish this limitation. the 1-h accuprobe chemiluminescent culture identification test for listeria monocytogenes was used as a model. its high detection threshold provides a stringent challenge for evaluating enrichment work-up protocols. detection of l. monocytogenes, at 1-4 colony-forming units/g f ...200616512244
probiotic lactobacillus casei activates innate immunity via nf-kappab and p38 map kinase signaling pathways.probiotic bacteria are microorganisms that benefit the host through improvement of the balance of intestinal microflora and possibly by augmentation of host defense systems. we examined the mechanisms for the up-regulation of innate immune responses by a probiotic lactobacillus casei atcc27139, in vivo. using mouse models of systemic listeria monocytogenes infection and metha fibrosarcoma tumorigenesis in combination with balb/c and scid mice, we found that parenteral administration of l. casei ...200616513392
colon cancer cells: pro-invasive signalling.colon cancer results from erroneous renewal of the enteric epithelium. mutations in stem cells, or their proliferative progenitors, cause accumulation of cells that invade into the stroma and continue to divide rather than migrating on top of the basement membrane prior to entering into apoptosis. many of these changes in invasive activity appear to be related to the invasion-suppressor role of e-cadherin. we have also investigated listeria monocytogenes and other enteric bacteria, since these b ...200616513408
genetic and phenotypic characterization of listeria monocytogenes lineage iii.listeria monocytogenes has been previously grouped into three evolutionary groups, termed lineages i, ii and iii. while lineages i and ii are commonly isolated from various sources, lineage iii isolates are rare and have several atypical and unique phenotypic characteristics. relative to their prevalence in other sources, lineage iii strains are overrepresented among isolates from food-production animals, and underrepresented among isolates from human clinical cases and foods. this work describe ...200616514149
[infliximab therapy in crohn's disease complicated by listeria monocytogenes meningoencephalitis]. 200616514404
comparison of two chromogenic media for the detection of listeria monocytogenes with the plating media recommended by en/din 11290-1.the efficacy of two selective chromogenic culture media, agar listeria ottaviani and agosti (aloa) and rapid' l. mono for the detection of listeria monocytogenes in food, was compared with that of an official culture method according to the en/din 11290-01 and -02 protocols [corresponding to the section 35 lmbg (german food act) method]. a total of 310 pre-packed ready-to-eat food samples (100 of graved and cold smoked salmon, 130 of different raw and cooked sausages and 80 of delicatessen and m ...200616515816
heterologous expression of betl, a betaine uptake system, enhances the stress tolerance of lactobacillus salivarius ucc118.given the increasing commercial and clinical relevance of probiotic cultures, improving the technological robustness of what are often process-sensitive cultures is an important biological goal. the nisin-controlled expression system was used to direct the heterologous expression of the listerial betaine uptake system betl in the probiotic strain lactobacillus salivarius ucc118. following nisin induction, strains expressing betl exhibited a significant increase in resistance to several stresses, ...200616517668
tolerance of listeria monocytogenes to cell envelope-acting antimicrobial agents is dependent on sigb.mutation of sigb impairs the ability of listeria monocytogenes to grow in sublethal levels, and to survive in lethal concentrations, of the bacteriocins nisin and lacticin 3147 and the antibiotics ampicillin and penicillin g. sigb may therefore represent an attractive target for the development of new control and treatment strategies for this important pathogen.200616517678
cutting edge: the direct action of type i ifn on cd4 t cells is critical for sustaining clonal expansion in response to a viral but not a bacterial infection.the action of type i ifn (ifn-i) on apcs is well studied, but their direct effect on cd4 t cells is unclear. to address this, we transferred ifn-i receptor-deficient (ifn-ir(0)) and -sufficient (wild-type, wt) tcr-transgenic cd4 t cells into wt mice and analyzed their response to immunization. in response to lymphocytic choriomeningitis virus immunization, wt cd4 t cells expanded approximately 100-fold, whereas ifn-ir(0) cd4 t cells expanded <10-fold. however, both wt and ifn-ir(0) cd4 t cells e ...200616517698
neonatal septicemia in high risk babies in south-eastern nigeria.septicemia is one of the major causes of morbidity and mortality in the neonatal period and it often has a rapid and fulminant course.200616519624
veterinary and medical aspects of abortion in danish sheep.the danish sheep population totals around 144,000 animals, but little is known of the causes and prevalance of diseases. this study focuses on the causes of abortion in danish sheep. during one breeding season, aborted foetuses and stillbirths with signs of intrauterine death or malformation were submitted for laboratory examination from a population of 3,758 breeding ewes. samples from 24 incidents of abortion and 21 ewes delivering malformed lambs or lambs with ante partum decomposition were s ...200616519752
invasiveness is a variable and heterogeneous phenotype in listeria monocytogenes serotype strains.the ability of listeria monocytogenes to breach mucosal and endothelial barriers of the host during infection is a hallmark property mediated by the internalins (inl) a and b. we examined the invasive property of several l. monocytogenes strains representing 13 serotypes. we found that invasiveness is a heterogeneous phenotype amongst l. monocytogenes serotype strains. despite this, many of the poorly invasive and non-invasive strains of l. monocytogenes express internalins at levels comparable ...200616527541
the ferritin-like protein frm is a target for the humoral immune response to listeria monocytogenes and is required for efficient bacterial survival.the identity and role of listerial antigens recognized by antibodies following listerial infection is largely unknown. we identified the listerial ferritin-like protein frm as a target of the humoral response following infection of mice with pathogenic listeria monocytogenes. specific antibodies to frm are detected in antisera of mice infected with a pathogenic strain but not in antisera of mice infected with a non-pathogenic l. innocua strain. antibodies raised to purified listerial ferritin al ...200616528570
[agglutination as screening test in routine diagnostic of listeriosis].the persons with weakened immunity, newborn infants and pregnant women are very much sensitive on infection with bacteria listeria monocytogenes. listeria can readily invade the placenta and precipitate premature labor and fetal death. to confirm diagnosis is very difficult because listeriosis is disease very similar to the others acute diseases. serum threshold values above 1: 320 confirm think about listeriosis when the relevant clinical symptoms are present. of greater diagnostic relevance is ...200616528925
genotypic and phenotypic heterogeneity in enterococcus isolates from batzos, a raw goat milk cheese.this study investigated the genotypic and phenotypic diversity in 34 isolates of enterococci obtained during ripening of batzos cheese from raw goat milk and characterized phenotypically as enterococcus durans. rapd-pcr, plasmid profiling and pfge were used to study the genetic variability and distinguish closely related isolates. species recognition by means of rapd-pcr was in agreement with the phenotypic identification for 29 strains. one strain was characterized as lactococcus lactis subsp. ...200616529834
[functional activity of mouse peritoneal macrophages contaminated with gram-positive bacteria].the analysis of the functional and enzymatic activity of mouse peritoneal macrophages contaminated with staphylococcus aureus and listeria monocytogenes virulent strains is presented. the low bactericidal and digestive activity of these cells with respect to the above-mentioned microorganisms was determined. in this study a decrease in the activity of plasmatic membrane enzymes (5'-nucleotidase and atp-ase) of macrophages contaminated with s. aureus and l. monocytogenes was observed, which was i ...200616532641
role of lung surfactant in phagocytic clearance of apoptotic cells by macrophages in rats.two of the common features of inflammatory lung diseases are the increased production of pulmonary surfactant and the induction of lung cell apoptosis. however, the relationship between these two events has not been addressed. to investigate the role of surfactant in pulmonary inflammation and apoptosis, we instilled natural lung surfactant (survanta) (1.6-12.5 mg) into the rat lungs and determined the number of alveolar macrophages (ams) and apoptotic lung cells. high-dose treatments of survant ...200616534500
consumer phase risk assessment for listeria monocytogenes in deli meats.the foodborne disease risk associated with the pathogen listeria monocytogenes has been the subject of recent efforts in quantitative microbial risk assessment. building upon one of these efforts undertaken jointly by the u.s. food and drug administration and the u.s. department of agriculture (usda), the purpose of this work was to expand on the consumer phase of the risk assessment to focus on handling practices in the home. one-dimensional monte carlo simulation was used to model variability ...200616492183
risk assessment of listeria monocytogenes: impact of individual cell variability on the exposure assessment step.recently, the lag phase research in predictive microbiology is focusing more on the individual cell variability, especially for pathogenic microorganisms that typically occur in very low contamination levels, like listeria monocytogenes. in this study, the effect of this individual cell lag phase variability was introduced in an exposure assessment study for l. monocytogenes in a liver pâté. a basic framework was designed to estimate the contamination level of pâté at the time of consumption, ta ...200616492184
the onset of cd8+-t-cell contraction is influenced by the peak of listeria monocytogenes infection and antigen display.the cd8+-t-cell response to infection with listeria monocytogenes consists of expansion, contraction, and memory phases. the transition between expansion and contraction is reported to occur on different days postinfection with virulent (8 to 9 days) and attenuated (deltaacta) (7 days) l. monocytogenes strains. we hypothesized that differences in the infectious courses, and therefore antigen (ag) display, determine the precise time of the expansion/contraction transition in response to these inf ...200616495523
induced biliary excretion of listeria monocytogenes.listeria monocytogenes is a ubiquitous gram-positive bacterium that can cause systemic and often life-threatening disease in immunocompromised hosts. this organism is largely an intracellular pathogen; however, we have determined that it can also grow extracellularly in animals, in the lumen of the gallbladder. the significance of growth in the gallbladder with respect to the pathogenesis and spread of listeriosis depends on the ability of the bacterium to leave this organ and be disseminated to ...200616495556
characteristics of listeria monocytogenes isolated from imported meat in japan.the genomic structure of the iap region in listeria monocytogenes (serovar 4b), isolated from chicken imported into japan, was compared with those from japanese strains. the isolate was similar to the japanese strains in a comparatively new, rare group. such strains might be imported from foreign countries.200616495636
[neonatal maculopapular exanthema revealing septicemia due to klebsiella pneumoniae via maternofetal infection].neonatal exanthema of bacterial origin is caused very rarely by gram-negative bacilli. we report a case of neonatal maculopapular exanthema evocative of klebsiella pneumoniae septicemia resulting from maternofetal infection.200616495848
pathogen reduction in unpasteurized apple cider: adding cranberry juice to enhance the lethality of warm hold and freeze-thaw steps.u.s. food and drug administration (fda) regulations require processors of apple cider sold wholesale to use processing steps that ensure a 5-log reduction in numbers of the pertinent pathogen, generally considered to be escherichia coli o157:h7. current widely used validated pathogen-reduction steps are thermal pasteurization and uv light treatment. these techniques may be unaffordable or undesirable for some processors. this study investigated the cran-cider process, which is the addition of cr ...200616496568
attributing risk to listeria monocytogenes subgroups: dose response in relation to genetic lineages.the objective of this study was to evaluate the hypothesis that the dose-response relationship for listeria monocytogenes in humans varies with genotypic lineage or subtype. the linkages between molecular subtyping data and enumeration data for l. monocytogenes subtypes in foods consumed by the at-risk population were examined to test this hypothesis. we applied a conditional probability model to conduct a subtype-specific dose-response analysis, with the focus on invasive listeriosis. l. monocy ...200616496574
a solid agar overlay method for recovery of heat-injured listeria monocytogenes.a solid agar overlay method was developed for recovery of heat-injured listeria monocytogenes. presolidified nonselective tryptic soy agar with 0.6% yeast extract (tsaye, 2% agar) was overlaid on top of solidified modified oxford agar (mox). heat injury of l. monocytogenes was conducted at 58 degrees c for 6 min in a jacketed flask filled with tryptic soy broth. both noninjured and heat-treated l. monocytogenes cells were plated onto tsaye, mox, and tsaye-mox plates. no significant differences ( ...200616496588
acidified sodium chlorite treatment for inhibition of listeria monocytogenes growth on the surface of cooked roast beef.the effects of acidified sodium chlorite (asc) against listeria monocytogenes on the surface of cooked roast beef were investigated. l. monocytogenes, strain v7, serotype 1/2a, was inoculated at numbers of 6.0 log cfu/g onto 5-g cubes of cooked regular or spicy roast beef. the samples were allowed to air dry for 1 h. the cooked roast beef samples were dipped into asc or sprayed with asc solutions of 250, 500, 750, or 1,000 ppm, then placed in bags with or without a vacuum and refrigerated at 4 d ...200616496589
prevalence and genetic characterization of listeria monocytogenes in retail broiler meat in estonia.the prevalence and genetic diversity of listeria monocytogenes in raw broiler legs at the retail level in estonia were studied. a total of 240 raw broiler legs (120 from estonia and 120 of foreign origin, which had been imported to estonia from denmark, finland, hungary, sweden, and the united states) from 12 retail stores in the two largest cities in estonia (tallinn and tartu) were investigated from january to december 2002. of these, 70% were positive for l. monocytogenes. the prevalence of l ...200616496590
incidence of enterohemorrhagic escherichia coli, escherichia coli o157, salmonella, and listeria monocytogenes in retail fresh ground beef, sprouts, and mushrooms.the objective of this study was to determine the prevalence of enterohemorrhagic escherichia coli (ehec), e. coli o157, salmonella, and listeria monocytogenes in retail food samples from seattle, wash. a total of 2,050 samples of ground beef (1,750 samples), mushrooms (100 samples), and sprouts (200 samples) were collected over a 12-month period and analyzed for the presence of these pathogens. pcr assays, followed by culture confirmation were used to determine the presence or absence of each or ...200616496591
radiation processing to ensure safety of minimally processed carrot (daucus carota) and cucumber (cucumis sativus): optimization of dose for the elimination of salmonella typhimurium and listeria monocytogenes.minimally processed vegetables are in demand, because they offer convenience to consumers. however, these products are often unsafe because of possible contamination with pathogens, such as salmonella, escherichia coli o157:h7, and shigella species. therefore, this study was carried out to optimize the radiation dose necessary to ensure the safety of precut carrot and cucumber. decimal reduction doses (d-values) of salmonella typhimurium mtcc 98 were ca. 0.164 kgy in carrot samples and 0.178 kgy ...200616496592
surface pasteurization of vacuum-sealed precooked ready-to-eat meat products.pathogens may contaminate ready-to-eat meat products after cooking but before packaging. listeria monocytogenes is a formidable contaminant in the food processing environment and is relatively heat resistant compared with other non-spore-forming pathogens. as a consequence, this microorganism is commonly chosen for evaluation in postpackage pasteurization studies. the aim of this study was to review information on the thermal surface pasteurization of vacuum-sealed precooked ready-to-eat meat pr ...200616496595
[neonatal listeriosis].we present the case of a male with a neonatal listeria monocytogenes infection. its evolution was favorable with intravenous ampicillin and gentamicin. listeriosis is an infrequent cause of neonatal pustulosis. the infection is acquired from the mother after bacteremia with few symptoms (early-onset forms) or while passing through an infected birth canal (late-onset forms). a cytological study and the quick stain technique make fast diagnosis of potentially serious neonatal pustuloses possible.200616540055
concentrations and tracking of listeria monocytogenes strains in a seafood-processing environment using a most-probable-number enrichment procedure and randomly amplified polymorphic dna analysis.concentrations of environmental microflora and listeria monocytogenes were monitored at multiple environmental locations within a seafood-processing facility over the course of 6 months. concentrations of l. monocytogenes were determined using a most-probable-number (mpn) enrichment procedure. two floor drains had persistent low concentrations of l. monocytogenes (0.03 to >1,100 mpn/cm2). in comparison, concentrations of the other organisms in the drain were much higher (heterotrophic plate coun ...200616541676
influence of different histories of the inoculum on lag phase and growth of listeria monocytogenes in meat models.the aim of this study was to determine the effect of history of inoculum and preservatives on the lag phase and growth rate of listeria monocytogenes strains in meat products packaged under modified atmosphere conditions. inocula with different histories were added to meat models, and growth rate and lag phase of two strains of l. monocytogenes were measured at 5 and 10 degrees c. the meat model stored at 10 degrees c contained sodium lactate, but the model stored at 5 degrees c did not. the fiv ...200616541682
inactivation of microorganisms in milk and apple cider treated with ultrasound.nonthermal technologies are emerging as promising alternatives to heat treatment for food processing. ultrasound, defined as sound waves with a frequency greater than 20 khz, has proven bactericidal effects, especially when combined with other microbial-reduction strategies such as mild heating. in this study, ultrasound treatment (sonifier probe at 20 khz, 100% power level, 150 w acoustic power, 118 w/cm2 acoustic intensity) with or without the effect of mild heat (57 degrees c) was effective a ...200616541685
thermal resistance parameters for pathogens in white grape juice concentrate.the heat resistance of escherichia coli o157:h7, salmonella, and listeria monocytogenes that were in stationary phase, had been exposed to high osmotic pressure, or were acid adapted was evaluated in white grape juice concentrate (58 degrees brix, ph 3.3). the most heat-resistant cells of all three pathogens were those exposed to high osmotic pressure or in stationary phase. unlike in single-strength juices, in concentrate the acid-adapted cells for all three pathogens were less heat resistant t ...200616541686
electron beam and gamma irradiation effectively reduce listeria monocytogenes populations on chopped romaine lettuce.fresh, chopped romaine lettuce contaminated with a seven-strain cocktail of listeria monocytogenes (in a solution containing approximately 10(8) organisms per ml) that had attained a level of contamination of between 7 and 8 log cfu/g was packaged in 15-g samples. the lettuce was irradiated with a co60 source at 1.15 or 0.51 kgy and then stored at 4 degrees c. in addition, samples contaminated with isolated strains 16397, 0733, and 1992 were subjected to either electron beam irradiation at doses ...200616541687
inactivation of escherichia coli o157:h7, salmonella typhimurium dt104, and listeria monocytogenes on inoculated alfalfa seeds with a fatty acid-based sanitizer.alfalfa seeds were inoculated with a three-strain cocktail of escherichia coli o157:h7, salmonella enterica subsp. enterica serovar typhimurium dt104, or listeria monocytogenes by immersion to contain approximately 6 to 8 log cfu/g and then treated with a fatty acid-based sanitizer containing 250 ppm of peroxyacid, 1,000 ppm of caprylic and capric acids (emery 658), 1,000 ppm of lactic acid, and 500 ppm of glycerol monolaurate at a reference concentration of 1x. inoculated seeds were immersed at ...200616541689
transfer of listeria monocytogenes during mechanical slicing of turkey breast, bologna, and salami.a commercial delicatessen slicer was used as the vector for sequential quantitative transfer of listeria monocytogenes (i) from an inoculated slicer blade (approximately 10(8), 10(5), or 10(3) cfu per blade) to 30 slices of uninoculated delicatessen turkey, bologna, and salami, (ii) from inoculated product (approximately 10(8) cfu/cm2) to the slicer, and (iii) from inoculated product (10(8), 10(5), or 10(3) cfu/cm2) to 30 slices of uninoculated product via the slicer blade. cutting force and pro ...200616541694
effect of fat content on infection by listeria monocytogenes in a mouse model.an estimated 2,500 cases of listeriosis occur annually in the united states. listeriosis is particularly severe among pregnant women and immunocompromised individuals. little is known regarding the effect of the food matrix on the ability of l. monocytogenes to survive in the gastrointestinal tract and cause systemic infection. mice were inoculated with various doses of l. monocytogenes in skim milk, half & half, or whipping cream to determine whether differences in milk fat content influence th ...200616541700
impact of the population of spoilage microflora on the growth of listeria monocytogenes on frankfurters.approximately 100 cfu/cm2 of a five-strain mixture of listeria monocytogenes was coinoculated onto frankfurters with three different concentrations (10(2), 10(4), and 106 cfu/cm2) of an undefined spoilage microflora derived from commercial frankfurters. the frankfurters were vacuum packaged and stored at 10 degrees c for up to 48 days. the populations of l. monocytogenes, aerobic mesophilic bacteria, lactic acid bacteria, and enterobacteriaceae were determined at various time intervals during st ...200616541704
cerebral listeriosis in an adult freiberger gelding.listeria monocytogenes infection, although rare in horses, can lead to septicaemia, gastroenteritis and abortion or stillborn foals. equine cerebral listeriosis has been reported, but only in newborn animals. this report describes a freiberger gelding with severe neuronal symptoms of sudden onset. the animal collapsed within 24 h and was humanely killed. necropsy revealed multiple small brown to reddish foci within the brain stem and pons. histopathology demonstrated multifocal suppurative menin ...200616542674
[molecular epidemiology of listeria monocytogenes infections in a health district of madrid in a 3-year period (2001-2003)].during 2003, an increase in the number of cases of listeriosis was observed in a tertiary hospital in madrid. the objectives of this study were to review the clinical characteristics of the cases diagnosed from 2001 to 2003 and to investigate clonal relationships among the clinical isolates of listeria monocytogenes.200616545315
distinct roles of tlr2 and the adaptor asc in il-1beta/il-18 secretion in response to listeria monocytogenes.apoptosis-associated speck-like protein containing a c-terminal caspase recruitment domain (asc) is an adaptor molecule that has recently been implicated in the activation of caspase-1. we have studied the role of asc in the host defense against the intracellular pathogen listeria monocytogenes. asc was found to be essential for the secretion of il-1beta/il-18, but dispensable for il-6, tnf-alpha, and ifn-beta production, in macrophages infected with listeria. activation of caspase-1 was abolish ...200616547271
chronic-contained rupture of an infected aneurysm of the abdominal aorta due to listeria monocytogenes.we report a case of chronic-contained rupture of an infected aneurysm of the abdominal aorta, from which listeria monocytogenes was cultured. the diagnosis of rupture and retroperitoneal mass was made by computed tomography, whereas fdg -pet diagnosed vessel wall inflammation. the infectious nature only became apparent at surgery.200616547362
lymphocytes are detrimental during the early innate immune response against listeria monocytogenes.mice deficient in lymphocytes are more resistant than normal mice to listeria monocytogenes infection during the early innate immune response. this paradox remains unresolved: lymphocytes are required for sterilizing immunity, but their presence during the early stage of the infection is not an asset and may even be detrimental. we found that lymphocyte-deficient mice, which showed limited apoptosis in infected organs, were resistant during the first four days of infection but became susceptible ...200616549598
directed antigen delivery as a vaccine strategy for an intracellular bacterial pathogen.we have developed a vaccine strategy for generating an attenuated strain of an intracellular bacterial pathogen that, after uptake by professional antigen-presenting cells, does not replicate intracellularly and is readily killed. however, after degradation of the vaccine strain within the phagolysosome, target antigens are released into the cytosol for endogenous processing and presentation for stimulation of cd8(+) effector t cells. applying this strategy to the model intracellular pathogen li ...200616549792
[meningitis due to listeria monocytogenes after spinal anesthesia]. 200616553352
effect of a bacteriocin-activated polythene film on listeria monocytogenes as evaluated by viable staining and epifluorescence microscopy.to evaluate the effect of a bacteriocin-activated polythene film on resting and growing populations of listeria monocytogenes.200616553731
a newly developed assay to study the minimum inhibitory concentration of satureja spinosa essential oil.the minimum inhibitory concentration (mic) of satureja spinosa essential oil against staphylococcus aureus, escherichia coli o157:h7, listeria monocytogenes, salmonella enterica, salmonella serovar enteritidis pt4 and bacillus cereus was comparatively assessed with an established optical density method as well as a novel impedimetric method.200616553733
single cell variability of l. monocytogenes grown on liver pâté and cooked ham at 7 degrees c: comparing challenge test data to predictive simulations.the variability in growth between individual listeria monocytogenes cells was investigated on liver pâté and cooked ham. these results were compared to monte carlo simulations based on data collected previously in broths (francois et al., submitted for publication).200616553736
associated roles of hemolysin and p60 protein for the intracellular growth of bacillus subtilis.hemolysin expressing bacillus subtilis strain (b. subtilis ble/hla) was used as a carrier for listerial protein p60 to study the impact of this protein on bacterial virulence independent of other gene products of listeria monocytogenes. bacillus subtilis ble/hlya exhibited longer cell chains than b. subtilis ble/hlya/iap. recombinant bacillus strains are able to adhere to the mouse macrophage-like j774 and human epithelial-like int407 cell lines. the bacterial number of b. subtilis ble/hlya/iap ...200616553805
characterization of ers, a prfa-like regulator of enterococcus faecalis.we have identified a transcriptional regulator, named ers (for enterococcal regulator of survival), of enterococcus faecalis, an important opportunistic bacterium commonly recovered from hospitalized patients. ers is a member of the crp/fnr family and is 69% similar to srv, a prfa-like regulator of streptococcus pyogenes implicated in virulence, and is the e. faecalis protein most closely related to prfa, a positive regulator of virulence genes in listeria monocytogenes. in an in vivo-in vitro m ...200616553815
detection of escherichia coli o157:h7, salmonella spp., staphylococcus aureus and listeria monocytogenes in kimchi by multiplex polymerase chain reaction (mpcr).we developed an mpcr assay for the simultaneous detection, in one tube, of escherichia coli o157:h7, salmonella spp., staphylococcus aureus and listeria monocytogenes using species-specific primers. the mpcr employed the e. coli o157:h7 specific primer stx2a, salmonella spp. specific primer its, s. aureus specific primer cap8a-b and l. monocytogenes specific primer hly. amplification with these primers produced products of 553, 312, 405 and 210 bp, respectively. all pcr products were easily dete ...200616554723
essential role of tnf family molecule light as a cytokine in the pathogenesis of hepatitis.light is an important costimulatory molecule for t cell immunity. recent studies have further implicated its role in innate immunity and inflammatory diseases, but its cellular and molecular mechanisms remain elusive. we report here that light is upregulated and functions as a proinflammatory cytokine in 2 independent experimental hepatitis models, induced by concanavalin a and listeria monocytogenes. molecular mutagenesis studies suggest that soluble light protein produced by cleavage from the ...200616557300
ccpc-dependent regulation of citb and lmo0847 in listeria monocytogenes.in bacillus subtilis, the catabolite control protein c (ccpc) plays a critical role in regulating the genes encoding the enzymes of the tricarboxylic acid branch of the krebs citric acid cycle. a gene encoding a potential ccpc homolog and two potential target genes were identified in the listeria monocytogenes genome. in vitro gel mobility shift assays and dnase i footprinting experiments showed that l. monocytogenes ccpc (ccpc(lm)) interacts with the promoter regions of citb(lm) (the gene that ...200616352834
innate immune responses in nf-kappab-repressing factor-deficient mice.nf-kappab-repressing factor (nrf) is a transcriptional silencer protein that specifically counteracts the basal activity of several nf-kappab-dependent promoters by direct binding to specific neighboring dna sequences. in cell culture experiments, the reduction of nrf mrna leads to a derepression of beta interferon, interleukin-8, and inducible nitric oxide synthase transcription. the x chromosome-located single-copy nrf gene is ubiquitously expressed and encodes a protein of 690 amino acids. th ...200616354699
listeria monocytogenes serotype 4b strains belonging to lineages i and iii possess distinct molecular features.a collection of listeria monocytogenes serotype 4b strains belonging to lineages i and iii were examined by pcr and southern blot analysis using species-, virulence-, and serotype-specific primers and probes. whereas four serotype 4b lineage i strains reacted in pcr with the serotype 4b-, 4d-, and 4e-specific orf2110 and virulence-specific lmo1134 and lmo2821 primers, all nine serotype 4b lineage iii strains were negative by orf2110 and lmo1134 primers. in addition, the nine serotype 4b lineage ...200616390972
fourier transform infrared and raman spectroscopy for characterization of listeria monocytogenes strains.the purpose of this study was to characterize the variation in biochemical composition of 89 strains of listeria monocytogenes with different susceptibilities towards sakacin p, using fourier transform infrared (ftir) spectroscopy and raman spectroscopy. the strains were also analyzed using amplified fragment length polymorphism (aflp) analysis. based on their susceptibilities to sakacin p, the 89 strains have previously been divided into two groups. using the ftir spectra and aflp data, the str ...200616391047
inhibition of listeria monocytogenes by food-borne yeasts.many bacteria are known to inhibit food pathogens, such as listeria monocytogenes, by secreting a variety of bactericidal and bacteriostatic substances. in sharp contrast, it is unknown whether yeast has an inhibitory potential for the growth of pathogenic bacteria in food. a total of 404 yeasts were screened for inhibitory activity against five listeria monocytogenes strains. three hundred and four of these yeasts were isolated from smear-ripened cheeses. most of the yeasts were identified by f ...200616391059
persistence of mycobacterium avium subsp. paratuberculosis and other zoonotic pathogens during simulated composting, manure packing, and liquid storage of dairy manure.livestock manures contain numerous microorganisms which can infect humans and/or animals, such as escherichia coli o157:h7, listeria monocytogenes, salmonella spp., and mycobacterium avium subsp. paratuberculosis (mycobacterium paratuberculosis). the effects of commonly used manure treatments on the persistence of these pathogens have rarely been compared. the objective of this study was to compare the persistence of artificially inoculated m. paratuberculosis, as well as other naturally occurri ...200616391093
competitive fitness of listeria monocytogenes serotype 1/2a and 4b strains in mixed cultures with and without food in the u.s. food and drug administration enrichment protocol.thirteen different serotypes of the food-borne pathogen listeria monocytogenes have been described. serotype 4b strains are most often associated with illness, and serotype 1/2a strains are most often isolated from foods and processing plants. different abilities to respond to stresses have been described for serotype 4b and 1/2a strains. one of the common enrichment protocols used to test foods for the presence l. monocytogenes is described in the u.s. food and drug administration (fda) bacteri ...200616391118
tnf receptor type 2 (p75) functions as a costimulator for antigen-driven t cell responses in vivo.naive t cells require costimulation for robust ag-driven differentiation and survival. members of the tnfr family have been shown to provide costimulatory signals conferring survival at distinct phases of the t cell response. in this study, we show that cd4 and cd8 t cells depend on tnfr type 2 (p75) for survival during clonal expansion, allowing larger accumulation of effector cells and conferring protection from apoptosis for a robust memory pool in vivo. we demonstrate using the mhc class i-r ...200616393990
bovine peptidoglycan recognition protein-s: antimicrobial activity, localization, secretion, and binding properties.peptidoglycan (pgn) recognition proteins (pgrps) are pattern recognition molecules of innate immunity that are conserved from insects to humans. various pgrps are reported to have diverse functions: they bind bacterial molecules, digest pgn, and are essential to the toll pathway in drosophila. one family member, bovine pgn recognition protein-s (bpgrp-s), has been found to bind and kill microorganisms in a pgn-independent manner, raising questions about the identity of the bpgrp-s ligand. addres ...200616394004
therapy of paraneoplastic pemphigus with rituximab: a case report and review of literature.paraneoplastic pemphigus (pnp) is an autoimmune blistering disease with poor prognosis when associated with malignant neoplasm. we report the case of a patient with pnp associated with a cd20+ non-hodgkin follicular lymphoma who was treated with rituximab plus corticosteroids and short courses of cyclosporin. one and a half years after rituximab therapy, oral ulcerations had cleared and oral methylprednisolone was slowly tapered down without further recurrences. in the course of the disease, the ...200616405612
multiple characterizations of listeria monocytogenes sensitive and insensitive variants to divergicin m35, a new pediocin-like bacteriocin.divergicin m35 is a new class iia bacteriocin produced by carnobacterium divergicin m35. the bactericidal activity of this antimicrobial peptide was tested against a set of 11 strains of listeria monocytogenes isolated from food.200616405682
partial characterization of enterocin mr99 from a corn silage isolate of enterococcus faecalis.to assess the inhibitory activity on gram-positive and gram-negative bacteria of several species of enterococci recovered from a natural corn silage.200616405692
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