Publications

salience and the effects of cs preexposure on aversion conditioning.	rats (n = 84) received preexposure to distilled water or to one of two differently salient flavors, 5.0% casein or 10.0% sucrose, casein being the more salient. each preexposure group then received aversion conditioning to a 5.0% casein or a 10.0% sucrose cs. aversion effects were reliably more enduring to casein than to sucrose. relative to water-preexposed groups, preexposure to casein attenuated aversion effects to the casein cs reliably less than preexposure to sucrose attenuated aversion ef ...	1988	2849411
similarity between human retinoic acid receptor and escherichia coli homoserine kinase.		1988	2849427
a new class of free radical scavengers reducing adriamycin mitochondrial toxicity.	beef heart mitochondria were incubated with adm and nadh. an adriamycin semiquinone radical was detected using esr spectroscopy. the semiquinone radical production rate is decreased upon addition of a scavenger (ad 20) in the reaction medium. nmri mice were treated with ad 20 (70 mg/kg, i.p.) 15 min prior adm injection (20 mg/kg, i.p.) or with adm alone. heart mitochondria were isolated 48 hr later. the enzymatic activities of complex i-iii and complex iv of the mitochondrial respiratory chain w ...	1988	2849451
the cupric site in nitrous oxide reductase contains a mixed-valence [cu(ii),cu(i)] binuclear center: a multifrequency electron paramagnetic resonance investigation.	multifrequency electron paramagnetic resonance (epr) spectra of the cu(ii) site in nitrous oxide reductase (n2or) from pseudomonas stutzeri confirm the assignment of the low field g value at 2.18 consistent with the seven line pattern observed at 9.31 ghz, 10 k. s-band spectra at 20 k are better resolved than the x-band spectra recorded at 10 k. the features observed at 2.4, 3.4, 9.31 and 35 ghz are explained by a mixed-valence [cu(1.5)..cu(1.5)] s = 1/2 species with the unpaired electron deloca ...	1988	2849565
human rotavirus infection enhances invasiveness of enterobacteria in ma-104 cells.	to study the interaction between common pathogens causing infectious diarrhea in humans, ma 104 cell cultures were infected with human rotavirus and salmonella typhimurium or shigella flexneri or enteroinvasive escherichia coli (eiec) concomitantly. when ma-104 cells were preinfected with human rotavirus, invasiveness of s. typhimurium was significantly enhanced. the enhancement was evident after 48 h of virus preincubation. at this time virus specific antigens were demonstrated in the cell cult ...	1988	2850823
endotoxin-induced uveitis in rats: morphological and biochemical study.	inflammation induced by systemic injection of endotoxin can be a good model for endogenous uveitis since ocular inflammation is induced without manipulating the eye. we carried out morphological and biochemical studies in lewis rats to evaluate the breakdown of the blood-ocular barrier following injection of endotoxin (1 mg/rat) in footpads. vasodilation was observed as early as 3 hours and became maximum at 18-24 hours after the injection. unlike in the eye, no inflammatory changes were observe ...	1988	2853240
in vitro activity of azithromycin (cp-62,993), a novel macrolide, against enteric pathogens.	azithromycin (cp-62,993 [9-deoxy-9a-methyl-9a-aza-9a-homoerythromycin]) is a novel macrolide antimicrobial. in this study the in vitro activity of cp-62,993 has been determined against selected enteropathogens, including clostridium difficile, and compared with that of erythromycin. mics were determined using an agar incorporation technique in mueller-hinton medium, containing saponin-lysed horse blood at a final concentration of 10% v/v, with an inoculum of 10(4) cfu. cp-62,993 was considerably ...	1988	2854515
shiga toxin inhibits the anti-invasive effect of interferons.		1988	2450154
[relation of the immunosuppressivity of virulent shigella to the structure of o antigen].	the capacity of filtrates of virulent shigella cultures (vsc) and their different fractions to render avirulent strains, injected intraperitoneally into mice simultaneously with these filtrates, capable of suppressing immune response (delayed hypersensitivity) has been studied. among the fractions of vsc filtrates, the lipoid fraction soluble in the mixture of ethanol and ether has proved to be active. active factors can be extracted from vsc filtrates by means of immunosorbents obtained from an ...	1988	2452538
high-yield purification of a pp60c-src related protein-tyrosine kinase from human platelets.	a protein-tyrosine kinase (ptk, ec 2.7.1.112) from human platelets was purified with high yield. purification of the enzyme involved sequential chromatography on casein-agarose, tyrosine-agarose, heparin-sepharose and hydroxylapatite. the procedure resulted in substantially enriched 54/52 kda polypeptides on sds-polyacrylamide gel electrophoresis and a yield of about 25% in ptk activity. about 250 micrograms of purified protein could be obtained from 1 g of cell protein. the purification factor ...	1988	2453218
natural killer cell activation and interferon production by peripheral blood lymphocytes after exposure to bacteria.	we have previously shown that peripheral blood natural killer (nk) cells have significant levels of cytotoxic activity against shigella flexneri-infected hela cells. in this report, we show that nk cell activity against k562 tumor cells and shigella flexneri-infected hela cells can be greatly enhanced by preincubating peripheral blood lymphocytes (pbl) for 18 h with kanamycin-treated shigella flexneri or salmonella typhimurium. cell-free supernatants obtained from pbl-bacteria cultures contained ...	1988	2453465
activation of the mas oncogene during transfection of monoblastic cell line dna.	using the tumorigenicity assay, we observed an activation of the mas oncogene during transfection of human acute myelocytic leukemia (ctv-2) dna. respective transfectants contained amplified mas sequences characterized by rearrangements in 5' and 3' noncoding regions that were transcribed into a 3.3 kb mas rna species. our data represent the second report on activation of the mas gene.	1988	2453759
structural and immunochemical studies of the lipopolysaccharide from a new provisional serotype of shigella flexneri.	the chemical structure of the o-antigen of a proposed new provisional serotype of shigella flexneri has been determined. methylation analysis, glc-ms, 1h-nmr and 13c-nmr showed that the linear o-antigenic polysaccharide is the same as for all s. flexneri [kenne, l., lindberg, b., petersson, k. & romanowska, e. (1977) carbohydr. res. 56, 363-370]. a novel structural feature is that the disaccharide alpha-d-glcp-(1----2)-alpha-d-glcp is linked to o4 of the n-acetyl-glucosamine residue. (formula: s ...	1988	2458260
shigella flexneri invasion plasmid antigens b and c: epitope location and characterization with monoclonal antibodies.	invasion plasmid antigens b (ipab) and c (ipac) are associated with the ability of shigellae to invade cultured mammalian cells. monoclonal antibodies against ipab and ipac polypeptides were produced and used in a whole-cell enzyme-linked immunosorbent assay to show that both ipab and ipac polypeptides were exposed on the surface of virulent shigellae. moreover, these surface epitopes were shown to be highly conserved among different serotypes of shigella spp. and enteroinvasive escherichia coli ...	1988	2459066
bacterial epitopes involved in the induction of reactive arthritis.	a panel of documented arthritogenic shigella flexneri strains as well as an epidemic-associated non-arthritogenic shigella sonnei control was used to identify a 2-megadalton plasmid specific to the arthritogenic strains. the plasmid, phs-2, contains a dna sequence that encodes a 22-amino acid polypeptide encompassing a pentapeptide homologous to part of the polymorphic region of the alpha-1 domain of hla-b27. these results suggest that molecular mimicry between arthritogenic bacterial-encoded ep ...	1988	2462352
direct effects of alpha 2-adrenergic receptor stimulation on intravascular systemic capacity in the dog.	the role of alpha 2-adrenergic receptor stimulation in the regulation of systemic vascular capacity and venous return, a major determinant of cardiac output, is not well understood. with the influence of the central nervous system isolated from the systemic circulation, the direct peripheral vascular effects of two specific, chemically distinct alpha 2-adrenergic receptor agonists, uk 14,304 and b-ht 920, were investigated in 19 dogs on total cardiopulmonary bypass with constant arterial perfusi ...	1988	2895736
[the action of azepoxole on central alpha 2-adrenoreceptors].	the effect of adrenomimetics on the central alpha 2-adrenoreceptors responsible for mydriasis was studied. it was found that alpha 2-adrenomimetic azepoxole (bht-933) exerts no effect on the central alpha 2-adrenoreceptors providing mydriasis.	1988	2906881
identification of a unique lymphocyte subpopulation in the sheep uterus.	a panel of monoclonal antibodies was used to define the lymphocyte subpopulations in the sheep uterus at various stages of the oestrous cycle. a striking finding was that the majority of lymphocytes in the uterine and endometrial glandular epithelia belonged to a unique lymphocyte subpopulation that expressed the cd45r antigen but was negative for major histocompatibility complex (mhc) class ii molecules and expressed low or undetectable levels of the cd5 antigen. when examined under the electro ...	1988	2962930
a proposed mechanism of action for alg and atg in severe acquired aplastic anaemia.	in this study we have shown that various batches of anti-lymphocyte globulin (alg), anti-thymocyte globulin (atg) and human intravenous immunoglobulin (iv ig) all contain antibodies with the capacity to block lymphocyte receptors for the fc region of igg, i.e., fc gamma receptors. these antibodies may exert an effect on fc gamma receptor bearing suppressor t cell function in vivo. since t cells have been implicated in the pathogenesis of acquired severe aplastic anaemia, it is conceivable that a ...	1988	2967377
genetics and regulation of enterobactin genes in shigella flexneri.	although shigella flexneri possesses the genes for two siderophore systems, enterobactin and aerobactin, the enterobactin system is only rarely utilized. to investigate the regulation of enterobactin expression in s. flexneri, all of the genes specifically required for synthesis and transport of enterobactin were cloned from both an expressing (ent+) and a nonexpressing (ent-) strain. notable differences between the cloned genes included endonuclease restriction site changes and the presence of ...	1988	2973458
[clinical characteristics of dysentery today].		1988	2973539
myosin atpase activity during avian cardiac and skeletal muscle development.	the myosin atpase activity and myosin light chain composition in developing chick heart and skeletal muscles were studied and compared. embryonic myosin was purified and characterized from day 7 to day 19 of embryogenesis. embryonic cardiac myosin generally showed the same ca2+-activated myosin atpase activity level as the adult value. in comparison, pooled pectoralis and hindlimb skeletal muscles from day 10 through day 19 showed myosin atpase activities that were all significantly less than th ...	1988	2976104
apparent hyperalgesia in the mouse tail-flick test due to increased tail skin temperature after lesioning of serotonergic pathways.	the relationship between tail skin temperature and responsiveness to noxious radiant heat in the tail-flick test was investigated in mice. a significant negative correlation between tail skin temperature and tail-flick latency was found when the tail skin temperature was increased by elevating the ambient temperature. after intracerebroventricular injection of the serotonin neurotoxin 5,7-dihydroxytryptamine (5,7-dht, 80 micrograms) tail skin temperatures were increased and tail-flick latencies ...	1988	2976242
[rpo pathway as a possible recombination mechanism of the interaction between dna of transducing phages and the escherichia coli genome].	the conditions affecting recombination of dnas of transducing lambdoid rifd phages with the chromosome of escherichia coli k-12 reca in the region of homology were studied. in support to the previously obtained data, the int system was shown to take no part in the process. the homologous character of recombination interactions and their dependence on efficiency of transcription were demonstrated. it is therefore suggested that recombination takes the rpo pathway. similar peculiarities were revea ...	1988	2976700
[the possibility of using the demonstration of shigella antigens in the study of the epidemic process in dysentery].	the detection of shigella antigens in feces in accordance with the results of the passive hemagglutination test is more effective than the bacteriological method for finding out persons infected with shigella, especially in the absence of pronounced clinical manifestations of dysentery, and can be used in the study of the mechanism of the epidemic process. the examination of two groups, each consisting of permanent and temporary members, has revealed that the use of this method for detection of ...	1988	2977464
[the results of a seroepidemiologic study of newcastle dysentery].	differences in the monthly distribution in the number of seropositive individuals among children and adults in years with different intensity of the epidemic process have been revealed. immunity in cases of dysentery caused by s. newcastle reflects the yearly and seasonal activation of the epidemic process in this shigella infection both in children and adults, the seroconversion characteristics observed in the year of a high morbidity level being a more objective criterion indicating the beginn ...	1988	2977465
[experimental study of atherosclerosis. i. anti-atherosclerotic actions of chinese herb effective component 8501].		1988	2978250
the epidemiology of s. sonnei and s. flexneri in pima county, arizona: an exploratory study.	investigation of 189 cases of shigellosis reported to the pima county, arizona health department in 1986 revealed that 23 per cent of cases could be attributed to travel to mexico, and 10 per cent to day care attendees and their household contacts. no source of infection or high-risk activity could be demonstrated for 43 per cent of the cases. households in which s. flexneri occurred were more likely to be characterized by crowded living situations and to have no known source of infection.	1988	3044149
isolation of shiga toxin-resistant vero cells and their use for easy identification of the toxin.	shiga toxin-resistant vero cells were isolated by treatment of the cells with nitrosoguanidine. these mutant cells were not affected by shiga toxin at more than 1 microgram/ml, although the parent vero cells were sensitive to 25 pg of the toxin per ml. immunofluorescence studies showed that all the mutant cells had lost toxin-binding capacity. the cytotoxic activities of various bacterial cultures against the parent and mutant cells were compared. all samples from 10 strains of shigella dysenter ...	1988	3045003
congo red binding and salt aggregation as indicators of virulence in shigella species.	smooth strains of shigella dysenteriae type 1, shigella flexneri, shigella boydii, and shigella sonnei which form pigmented colonies (pcr+) on congo red agar were virulent in the sereny test. smooth variants unable to bind congo red (pcr-) were avirulent. measurements of dye uptake from solution showed that s. dysenteriae type 1 bound the most dye, followed in order of uptake by s. flexneri, s. boydii, and s. sonnei. using the salt aggregation test (sat) to determine cell surface hydrophobicity, ...	1988	3045151
clinical and microbiologic features of shigella and enteroinvasive escherichia coli infections detected by dna hybridization.	to determine the clinical and microbiologic features of shigella and enteroinvasive escherichia coli (eiec) infections, we investigated 410 children with diarrhea and 410 control children without diarrhea who were seen at children's hospital, bangkok, thailand, from january to june 1985. shigella spp. were isolated from 96 (23%) and eiec were isolated from 17 (4%) of 410 children with diarrhea and from 12 (3%) and 6 (1%) of 410 control children, respectively. the isolation rates of both pathogen ...	1988	3045152
glycocalyx in virulent and avirulent strains of shigella flexneri.		1988	3045162
[morphologic manifestations of the medicinal lysis of a coronary artery thrombus].	morphologic studies of coronary arterial thrombi have shown immature fibrin fibres to prevail in those, with fibrin degradation products always present. this fact suggests that fibrinolytic components of hemostasis, limiting the rates of fibrin polymerization and maturation, may participate in thrombogenesis. at the same time, macrophages that phagocytose fibrin and blood cells (platelets and erythrocytes) become activated since the early hours of the thrombus' existence. selective administratio ...	1988	3045402
[mechanism of the nonspecific protective action of shigella antigens].	the mechanism promoting the nonspecific action of antigens obtained from s. flexneri and s. sonnei by a sparing method has been studied. these antigens stimulate the t- and b- systems of immunity, that is followed by activation of myelopoiesis and the humoral protective factors of the body, which seems to underlie the formation of resistance to infection caused by nonspecific microorganisms.	1988	3046195
immunoreactivity of a surface wall fraction produced by spherules of coccidioides immitis.	the membranous spherule outer wall (sow) isolated from liquid cultures of coccidioides immitis has been shown to elicit reactivity with human anti-coccidioides antibody by immunofluorescence and the immunodiffusion-tube precipitin assay. the serologically reactive components were extracted from sow with the nonionic detergent n-octyl-beta-d-glucopyranoside (og). the og-soluble fraction of sow was shown to be reactive with immunoglobulin g in 25 serum samples from coccidioidomycosis patients by a ...	1988	3047063
immunoreactivity of a surface wall fraction produced by spherules of coccidioides immitis.	the membranous spherule outer wall (sow) isolated from liquid cultures of coccidioides immitis has been shown to elicit reactivity with human anti-coccidioides antibody by immunofluorescence and the immunodiffusion-tube precipitin assay. the serologically reactive components were extracted from sow with the nonionic detergent n-octyl-beta-d-glucopyranoside (og). the og-soluble fraction of sow was shown to be reactive with immunoglobulin g in 25 serum samples from coccidioidomycosis patients by a ...	1988	3047063
[lipid peroxidation in the pathogenetic and clinical aspects of infectious diseases].		1988	3047989
blood-injury phobia: a review.	natural human uneasiness about blood, injury, or deformity sometimes becomes a specific phobia, which can lead to serious disability if vital medical procedures are refused. blood-injury phobia usually starts in childhood and is often familial. unlike other phobic cues, which cause persistent tachycardia, blood-injury phobic cues evoke an initial rise in heart rate followed by vasovagal bradycardia and, frequently, syncope. although blood-injury phobia may have an evolutionary, genetic, and phys ...	1988	3048117
deletion of the shiga toxin gene in a chlorate-resistant derivative of shigella dysenteriae type 1 that retains virulence.	we used a probe specific for detecting the structural-gene sequences of shiga toxin to analyze the genetic nature of toxin synthesis in mutant derivatives of shigella dysenteriae type 1. a chlorate-resistant (chl) mutant (725-78) of s. dysenteriae type 1 strain 3818t, which had retained virulence but had lost production of high levels of cytotoxic activity associated with shiga toxin synthesis, contained a complete deletion of the shiga toxin structural-gene sequences. these structural-gene sequ ...	1988	3049838
[pro-allergy role of infection. a component of the mode of reacting].	no organism from the cradle lives germ-free or allergen-free. the modalities by which infections facilitate conditions of abnormal reactivity, in particular respiratory asthma and allergy to cow milk proteins, are examined. 216 asthmatic children and 50 infants with rotavirus enteritis have been considered. infections, besides representing stimuli directly projected on the immune system, also constitute factors which more generally influence the way of reacting.	1988	3050902
[differentiation of virulent strains of shigella and entero-invasive escherichia from their avirulent variants using modified indirect immunoenzyme analysis].	the data obtained in this investigation confirm that the modified indirect enzyme immunoassay (eia) permits the differentiation of virulent bacteria of the genus shigella and enteroinvasive escherichia (group 1), regularly containing virulence plasmids with a molecular weight of 120-140 md, from their avirulent variants which have lost these plasmids (group 2). the ratio of the optic density (od) values of the positive control samples (the od of group 1) to the od values of the negative ones (th ...	1988	3051808
the persistence of shigella flexneri in the united states: increasing role of adult males.	the annual reported isolation rate of shigella flexneri decreased from 1964 to 1973, but has remained constant since then at 1 per 100,000. between 1975 and 1985, the median age of males from whom s. flexneri was isolated rose from 5 to 26 years. during this time, the isolation rate of s. flexneri rose more than five-fold among men, did not change in adult women, and decreased in children. by 1985, 23 per cent of reported s. flexneri isolates came from men aged 20-49. increased male homosexual t ...	1988	3052115
the epidemiology of shigellosis in israel.	analysis of shigella isolates referred for serotyping to the central shigella reference laboratory has revealed a slight predominance of s. sonnei (47%) over s. flexneri (44%) in israel. in female patients, the predominance of s. sonnei isolates was more accentuated (54 v. 37.5% for s. flexneri). on the other hand, an increased incidence of s. flexneri was observed in military personnel (59 v. 32% for s. sonnei), and even more so in the arab population (69 v. 19%). incidence peaked in summer (ju ...	1988	3052334
the ecology and pathogenicity of urease-producing bacteria in the urinary tract.	urease activity is a physiological function of many bacteria that enables these organisms to utilize urea as a source of nitrogen. the association of ureolytic bacteria with human or animal hosts varies widely from a commensal relationship as demonstrated with skin microflora, a symbiotic relationship in the gastrointestinal tract, to a pathogenic relationship in the urinary tract. since similar or identical species of bacteria such as staphylococcus aureus are found in all three environments, t ...	1988	3053050
the ecology and pathogenicity of urease-producing bacteria in the urinary tract.	urease activity is a physiological function of many bacteria that enables these organisms to utilize urea as a source of nitrogen. the association of ureolytic bacteria with human or animal hosts varies widely from a commensal relationship as demonstrated with skin microflora, a symbiotic relationship in the gastrointestinal tract, to a pathogenic relationship in the urinary tract. since similar or identical species of bacteria such as staphylococcus aureus are found in all three environments, t ...	1988	3053050
shigella flexneri bacteraemia in post partum period.		1988	3053638
[a case using plasmapheresis in treating infectious toxicosis in young infants].		1988	3054796
[stability of shigella flexneri attenuated strain 2a 77 ade- rif-r after cultivation in a fermenter and lyophilization].	the method for the cultivation of s. flexneri attenuated strain 2a 77 ade- rifr in a fermenter with the use of casein fermentative broth containing 200-250 mg% of amino nitrogen has been developed. no changes in the properties of the initial strain have been found after its cultivation in a fermenter or after the lyophilization of the cultures grown in a fermenter. after cultivation in casein fermentative broth with 100 mg% of amino nitrogen the presence of s-r dissociation has been established, ...	1988	3055763
characterization of invasion plasmid antigen genes (ipabcd) from shigella flexneri.	the large invasion plasmid of shigella flexneri m9ot-w was used to generate recombinant plasmids carrying the ipaa, -b, -c, and -d genes, whose products are associated with the entry of the bacteria into colonic epithelial cells. complete dna sequences of ipab, -c, and -d were determined. the proteins predicted (62, 42, and 37 kda, respectively) from the nucleotide sequences lack a signal-peptide sequence. hydrophilic segments of the ipab and ipac proteins were found to overlap known epitopic do ...	1988	3057506
persistent shigellosis.	shigella usually causes a self-limited infection which untreated lasts on average seven days (range one to 30 days). we report a patient who had persistent symptoms for 10 months caused by shigella flexneri which was finally identified on cultures from a rectal swab.	1988	3058558
affinity chromatography of reca protein and reca nucleoprotein complexes on reca protein-agarose columns.	we have analyzed the nature of reca protein-reca protein interactions using an affinity column prepared by coupling reca protein to an agarose support. when radiolabeled soluble proteins from escherichia coli are applied to this column, only the labeled reca protein from the extract was selectively retained and bound tightly to the affinity column. efficient binding of purified 35s-labeled reca protein required mg2+, and high salt did not interfere with the binding of reca protein to the column. ...	1988	3058701
[etiologic structure of bacterial dysentery in the ussr in 1983-1985].	the etiological structure of dysentery in the ussr in 1983-1985 is characterized. sonne dysentery was found to prevail in the territories with adequate water supply, while dysentery caused by shigella flexneri prevailed at the territories with unsatisfactory water supply. s. dysenteriae and s. boydii were found to play a limited role in the etiology of dysentery. in the presence of global pandemic, an increase in the isolation rate of s. dysenteriae i in the ussr is observed. the data on the bio ...	1988	3061262
a 13c-n.m.r. study of some shigella flexneri o-polysaccharides.	13c-n.m.r. data for some shigella flexneri o-polysaccharides are reported. it is concluded that the chemical shifts observed for the linear shigella flexneri type y o-polysaccharide are similar to those calculated by adding substituent shifts, obtained from disaccharides with similar stereochemistry around the glycosidic linkage, to the chemical shifts for the monomers. the influence of substitution with alpha-d-glucopyranosyl and/or o-acetyl groups in different positions of this polysaccharide ...	1988	3061647
[pathogenetic significance of virulence-associated genes in shigella flexneri].		1988	3063851
interaction between vestibulocollic and cervicocollic reflexes: automatic compensation of reflex gain by muscle afferents.		1988	3064144
[electron microscopic study of the interaction of virulent and avirulent shigella flexneri strains with the macrophages of murine peritoneal exudate].	the electron microscopic dynamic study of the interaction of s. flexneri virulent strain 2a and s. flexneri avirulent strain, genetically related to strain 2a and having the same antigenic structure, with peritoneal macrophages of cba mice at an early period showed differences in the cytotoxic action of these strains. even at the early period of the interaction between macrophages and virulent shigellae structural changes were observed in macrophages: ruptures in the membranes of parasitophore p ...	1988	3064506
[scientific bases of epidemiologic surveillance of intestinal infections].		1988	3064508
arginyl-trna synthetase from escherichia coli, purification by affinity chromatography, properties, and steady-state kinetics.	a blue sephadex g-150 affinity column adsorbs the arginyl-trna synthetase of escherichia coli k12 and purifies it with high efficiency. the relatively low enzyme content was conveniently purified by deae-cellulose chromatography, affinity chromatography, and fast protein liquid chromatography to a preparation with high activity capable of catalyzing the esterification of about 23,000 nmol of arginine to the cognate trna per milligram of enzyme within 1 min, at 37 degrees c, ph 7.4. the turnover ...	1988	3064807
arginyl-trna synthetase from escherichia coli, purification by affinity chromatography, properties, and steady-state kinetics.	a blue sephadex g-150 affinity column adsorbs the arginyl-trna synthetase of escherichia coli k12 and purifies it with high efficiency. the relatively low enzyme content was conveniently purified by deae-cellulose chromatography, affinity chromatography, and fast protein liquid chromatography to a preparation with high activity capable of catalyzing the esterification of about 23,000 nmol of arginine to the cognate trna per milligram of enzyme within 1 min, at 37 degrees c, ph 7.4. the turnover ...	1988	3064807
serological response to plasmid-encoded antigens in children and adults with shigellosis.	the immunological response to plasmid-encoded antigens of virulent shigella was determined in thai children less than 4 yr of age and in thai adults by immunoblot analysis and elisa. forty-two percent (8/19) of thai children and 4% (1/22) of thai adults with shigellosis developed a greater than or equal to 4-fold rise in igg antibody titer to water-extracted antigens of shigella flexneri m90t by elisa (p = 0.006). two children and one lactating mother with shigellosis developed a 4-fold rise in ...	1988	3066570
regulation of urea cycle enzymes.		1988	3067149
[characteristics of the course of acute dysentery in a 5-year-old child complicated 3 times by intestinal invagination].		1988	3067205
physiological and behavioral aspects of stress in adolescence.	gonadal and adrenal hormone levels appear to be linked to the stressors experienced by young adolescents. adjustment problems were accompanied by a profile of lower gonadal steroids and higher adrenal androgen levels, primarily androstenedione. later gonadal maturation may be a result of stress suppressing the reproductive axis. higher levels of androstenedione may be indicative of chronic levels of stress. however, the findings for androstenedione are complicated by the fact that androstenedion ...	1988	3067560
genetic relatedness of the basic replicon of the virulence plasmid in shigellae and enteroinvasive escherichia coli.	the regions required for replication (rep) and for stable maintenance (stb) of the 230 kilobase virulence plasmid, pmysh6000, in shigella flexneri 2a ysh6000 were identified on the restriction enzyme sa/l fragments, c and o, respectively. the stable plasmid, pmysh6610, consisting of adjacent fragments c and o was found to belong to the incompatibility group fl. in all strains of shigellae and enteroinvasive escherichia coli (eiec) tested, virulence plasmids were found to be highly homologous wit ...	1988	3070262
[vitamin d as an immuno-hematopoietic hormone. new perspectives on a previously known substance].	1,25-dihydroxyvitamin d3 (1.25[oh]2d3), the most active metabolite of vitamin d3, plays an important role in bone and mineral metabolism. there is growing evidence that the vitamin d endocrine system may be important in other systems as well: 1,25(oh)2d3 is synthesized in extrarenal tissue. macrophages from both normal human individuals and patients with sarcoidosis are capable of producing 1,25(oh)2d3. specific receptors for 1,25(oh)2d3 are present in a number of cells and tissues other than th ...	1988	3070739
[the importance of secondary immune deficiencies in the pathogenesis of dysentery].	some immunity indices were studied in patients with acute dysentery. the number of total and active t-lymphocytes, t mu- and t gamma-cells, t-rabbit, t-staphylococcal, b-total and b-lymphocytes forming rosettes with mouse erythrocytes were determined for assessment of the immune status. changes in the above indices with relation to the severity of the disease were noted. suppression of the t- and b-systems of immunity were noticeable in patients with a severe and lingering course of disease. del ...	1988	3070808
[free-radical oxidation of blood plasma lipids in patients with acute intestinal infections].	blood plasma free radical lipid oxidation was studied in 98 patients with acute dysentery (ad) and 64 patients with food toxinfection (fti). free radical lipid oxidation was assessed by the intensity of chemiluminescence and the level of lipid peroxides. activation of blood plasma lipid peroxidation was established with relation to a clinical variant of the disease. the effect of indomethacin on the parameters of free radical lipid oxidation was noted in patients with ad and fti.	1988	3070810
nucleotide sequence of the invasion plasmid antigen b and c genes (ipab and ipac) of shigella flexneri.	the nucleotide sequence of a 4.8 kilobase (kb) hindiii fragment from pwr100, the virulence plasmid of shigella flexneri 5, was determined and analysed. this fragment encodes polypeptides b (62 kilodalton, kd) and c (43 kd) which have already been described as two of the four immunogenic polypeptides of shigellae. the nucleotide sequence revealed that in addition to the ipab and ipac genes encoding polypeptides b and c, a third complete open reading frame was found within the fragment. the gene, ...	1988	3071655
chronic shigella infection in an hiv seropositive patient--ontario.		1988	3072101
outbreak of shigella flexneri in a nursing home--alberta.		1988	3072103
plasmid mediated drug resistance in shigella flexneri serotypes 1-6 during 1974-85.		1988	3072296
[an analysis of distribution of shigella groups for thirty-seven years (1949-1985) in china].		1988	3073017
[evaluation of selective properties of mac conkey's medium enriched with potassium tellurite for growing shigella and yersinia strains].		1988	3073281
shigellemia in an infant with intractable diarrhea.		1988	3074059
laboratory studies on the mode of action and application of antiestrogen for breast cancer therapy.		1988	3074131
laboratory studies on the mode of action and application of antiestrogen for breast cancer therapy.		1988	3074131
[separation of shigella enterotoxin and immunosuppression factors].		1988	3074243
[detection and characterization of collagenolytic activity of serine proteases of the subtilisin type].		1988	3074903
[infantile shigella infections in region ix].		1988	3075065
effect of fermentation of ghanaian maize dough on the survival and proliferation of 4 strains of shigella flexneri.	fermented and non-fermented ghanaian maize dough was seeded with approximately 10(7) colony forming units of 4 strains of shigella flexneri which had been isolated from patients with dysentery. in the non-fermented maize dough (ph 6.2) the shigellae were detectable in large numbers for up to 24 h after exposure. in the maize dough that had been fermented for 3 d (ph 3.2) 3 strains were detectable in small numbers for up to 6 h after inoculation. thereafter none was isolated. the fourth strain, t ...	1988	3076718
antibiotic resistance among shigella species isolated in tehran, iran.	a study of adult patients with gastroenteritis was carried out over an 18-month period from march 1984 until august 1985, with the aim of detecting shigella species and studying their antibiotic resistance pattern. two thousand four hundred and eighteen stool specimens were examined and 230 isolations of shigella were made, a detection rate of 9.5%. of the 230 isolates, 61.2% were found to be sh. flexneri, and resistance to ampicillin, chloramphenicol, co-trimoxazole and tetracycline was extreme ...	1988	3077023
luteolytic potency of 16-phenoxy-derivatives of prostaglandin f2 alpha.	the binding of 16-phenoxy derivatives of prostaglandin (pg) f2 alpha to rat luteal membranes, and also their abortifacient potency in pregnant rats, have been studied. competitive binding studies with various pg-analogues were performed in ovaries of juvenile rats pretreated with pmsg and hcg, and in parallel studies the abortifacient potency of these substances was tested in pregnant rats. it was observed that this class of derivatives bound to the pgf2 alpha receptor as well as, or even better ...	1988	3162421
transforming growth factor-beta-induced growth inhibition and cellular hypertrophy in cultured vascular smooth muscle cells.	we have explored the hypothesis that hypertrophy of vascular smooth muscle cells may be regulated, in part, by growth inhibitory factors that alter the pattern of the growth response to serum mitogens by characterizing the effects of the potent growth inhibitor, transforming growth factor-beta (tgf-beta), on both hyperplastic and hypertrophic growth of cultured rat aortic smooth muscle cells. tgf-beta inhibited serum-induced proliferation of rat aortic smooth muscle cells (ed50 = 2 pm); this is ...	1988	3166463
a correlation between regional acetylcholinesterase activity in rat brain and performance in a spatial task.	the acquisition and retention of a water maze task was examined in 12 intact, young wistar rats. acetylcholinesterase activity in 43 discrete brain regions was then measured in the same rats by quantitative histochemistry. individual learning and retention indices were found to be significantly correlated with acetylcholinesterase (ache) levels in specific regions, e.g. cholinergic nuclei; the ventral pallidum and nucleus basalis; and in the dentate gyrus of the hippocampus. high levels of ache ...	1988	3166718
changes in vascular reactivity in experimental diabetic rats: comparison with hypothyroid rats.	the responsiveness to vasoactive agents in the perfused mesenteric vascular bed of streptozocin-induced diabetic rats was examined and compared with that of propylthiouracil-induced hypothyroid rats. diabetic rats at 4 and 8 weeks after the induction of diabetes showed a significant decrease in isoproterenol-induced vasodilatation. in addition, the contractile responses to norepinephrine and 5-hydroxytryptamine and the vasodilative response to acetylcholine were significantly decreased in 12-wee ...	1988	3167224
production of tumor necrosis factor in nude mice by muramyl peptides associated with bacterial vaccines.	gram-negative vaccines can elicit the production of tumor necrosis factor (tnf) in mice primed by muramyl dipeptide (mdp) or by its lipophilic derivative mdp-dipalmitoyl glycerol (mdp-gdp). in mice pretreated with mdp and particularly with mdp-gdp, bordetella pertussis vaccine was shown to be more effective than typhoid vaccine. the time course of tnf production in the blood did not indicate any difference between the effect of mdp or of mdp-gdp. in both cases the cytotoxic activity reached maxi ...	1988	3167834
production of human-human hybridomas secreting monoclonal antibodies reactive to breast cancer cell lines.	we produced human-human hybridomas by fusing lymph node lymphocytes of breast cancer patients with a fusion partner, ho-323 cells, in the presence of 50% polyethylene glycol, and screened hybridomas producing monoclonal antibody (moab) reactive to a breast cancer cell line, mcf-7. among 11 hybridomas secreting igm reactive to mcf-7, four hybridomas (h15a3, h15f2, k15b4, and k15c3) produced igm reacting specifically to breast cancer cell lines (mcf-7 and hbc-5). among them, h15a3 proliferated in ...	1988	3170451
effect of vagotomy on bile acid absorption by rat small intestine in vitro.		1988	3175250
multiple cysteine proteinase forms during the life cycle of dictyostelium discoideum revealed by electrophoretic analysis.	proteinases of the cellular slime mould dictyostelium discoideum have been analysed using electrophoresis on polyacrylamide gels containing gelatin (gelatin/page). multiple proteinase forms were apparent in vegetative myxamoebae, but the presence of individual enzyme forms depended on the manner in which the cells were grown. axenic cells had a characteristic a-pattern of proteinases consisting of six bands, the most active enzymes having apparent mr values of 51,000 and 45,000 (these have been ...	1988	3178750
activity of glucosidase inhibitors against hiv infections.		1988	3182426
heterogeneous colony forming ability in vitro of the abnormal clone-derived granulocyte-macrophage precursors in myelodysplastic syndromes.	the clonal origin of granulocyte-macrophage colony forming cells (cfu-gm) in the myelodysplastic syndrome (mds) was cytogenetically studied. chromosome analysis was carried out on single gm-colonies from six patients with mds, whose bone marrow cells had chromosome abnormalities. abnormal clone-derived cfu-gm were grown in four patients under the presence of human placental conditioned medium. in the remaining two, all analysed colonies revealed a normal karyotype, although the majority of metap ...	1988	3184986
measurement of local cerebral glucose utilization before and after v-p shunt in congenital hydrocephalus in rats.	local cerebral glucose utilization (lcgu) was measured by the [14c]deoxyglucose autoradiographic method in rats with congenital hydrocephalus before and after a v-p shunt. lcgu was decreased throughout the brain in hydrocephalus. the most severely affected regions were found in the cortex, with which a marked degree of subcortical cerebrospinal fluid (csf) edema was associated; regions in the brain-stem structures were less affected. these metabolic disturbances were improved in many regions aft ...	1988	3185424
thoracic cord compression in scheuermann's disease.	a 29-year-old man with spastic paraparesis of insidious onset was discovered to have thoracic cord compression by a combination of kyphosis and thoracic disc herniation. radiologic evaluation showed evidence of scheuermann's disease, of which thoracic kyphosis is one manifestation. neurologic signs are uncommon in scheuermann's disease and few cases have been successfully managed with resolution of neurologic deficit. this patient made a virtually complete recovery after thoracic cord decompress ...	1988	3187711
progressive ankylosis in mice. an animal model of spondylarthropathy. i. clinical and radiographic findings.	to determine its similarity to human spondylarthropathies, we studied murine progressive ankylosis, a spontaneously occurring disorder of joints in mice. clinically, peripheral joints were inflamed initially, then became ankylosed in a predictable sequence from distal to proximal. forefeet were involved before hindfeet. axial joint involvement produced severe spinal ankylosis. extraarticular manifestations included balanitis and crusting skin lesions. radiographically, bony erosions and calcific ...	1988	3190783
characterization of two deletions that remove the entire human zeta-alpha globin gene complex (- -thai and - -fil).	we have fully characterized two alpha thalassaemia mutants that occur in southeast asia, - -thai and - -fil. each mutant is due to a deletion that removes the entire zeta-alpha-globin gene complex. localization of the 5' breakpoints described here, allows the identification of unique fragments that are specific for each of the two mutations. this information can be used to assess the frequency of these mutants in southeast asia and will be of value in prenatal testing for alpha thalassaemia in t ...	1988	3191033
[effect of the induction of liver cytochrome p-450 by a chemically inert fluorocarbon on the reactions of immediate and delayed hypersensitivity].	the effect of chemically inert perfluorodecalin (pfd) on the development of hypersensitive reactions of immediate and delayed types in guinea pigs and mice was studied. it was found that administration of pfd to animals both before sensitization (mice) and during the development of sensitization (guinea pigs) reduced the degree of the both types of hypersensitivity.	1988	3191983
mobile sequences in the pyruvate dehydrogenase complex, the e2 component, the catalytic domain and the 2-oxoglutarate dehydrogenase complex of azotobacter vinelandii, as detected by 600 mhz 1h-nmr spectroscopy.	600 mhz 1h-nmr spectroscopy demonstrates that the pyruvate dehydrogenase complex of azotobacter vinelandii contains regions of the polypeptide chain with intramolecular mobility. this mobility is located in the e2 component and can probably be ascribed to alanine-proline-rich regions that link the lipoyl subdomains to each other as well as to the e1 and e3 binding domain. in the catalytic domain of e2, which is thought to form a compact, rigid core, also conformational flexibility is observed. i ...	1988	3191993
the disposition of [2,3-14c]-methyl and [2,3-14c]-2-ethylhexyl acrylate in male wistar albino rats.	the disposition of methyl [2,3-14c]-acrylate (ma) and 2-ethylhexyl [2,3-14c]-acrylate (eha) following intraperitoneal and oral administration to rats has been studied. the 14c found in the tissues was mainly associated with liver, kidneys and lungs. loss of 14c from these tissues occurred fairly rapidly, excluding the rats given eha intraperitoneally. most of the administered acrylates underwent rapid metabolism and excretion with expired air (more than 50% of the dose and urine (10-50% of the d ...	1988	3196152
dog owners and their use of veterinary services.		1988	3198454
phaeohyphomycosis caused by alternaria alternata in a cat.	phaeohyphomycosis caused by alternaria alternata was diagnosed in a 6-year-old cat. a lesion in the nose resulted in enlargement of the dorsum of the nose. similar appearing lesions had been removed from the dorsum of the nose 1 and 4 years earlier. the lesion recurred 3 months after surgical excision and irregular administration of ketoconazole. a second cytoreductive operation followed by 5 months' treatment with ketoconazole resolved the infection. nasal trauma occurring at 8 months and at 5 ...	1988	3198465