Publications

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[new data on the role of thermostable soluble toxin produced by bacillus thuringiensis berliner]. 196013689013
the production by bacillus thuringiensis berliner of a heat-stable substance toxic for insects. 195913638893
[biological titration of basic preparations of bacillus thuringiensis berliner in relation to anagasta (ephestia) kuhniella zell]. 195913806070
[biological titration of preparations of spores of bacillus thuringiensis berliner]. 195813609014
the protein crystals of bacillus thuringiensis berliner. 195513270146
[study of a strain of bacillus, pathogenic for certain insects, identifiable with bacillus thuringiensis berliner]. 195513302859
chemical and biological insecticides select distinct gene expression patterns in aedes aegypti mosquito.worldwide evolution of mosquito resistance to chemical insecticides represents a major challenge for public health, and the future of vector control largely relies on the development of biological insecticides that can be used in combination with chemicals (integrated management), with the expectation that populations already resistant to chemicals will not become readily resistant to biological insecticides. however, little is known about the metabolic pathways affected by selection with chemic ...025540155
cadherin binding is not a limiting step for bacillus thuringiensis subsp. israelensis cry4ba toxicity to aedes aegypti larvae.bacillus thuringiensis subsp. israelensis produces three cry toxins (cry4aa, cry4ba and cry11aa) that are active against aedes aegypti larvae. the identification of the rate-limiting binding steps of cry toxins that are used for insect control in the field, such as those of b. thuringiensis subsp. israelensis, should provide targets for improving insecticides against important insect pests. previous studies showed that cry11aa binds to cadherin receptor fragment cr7-11 (cadherin repeats 7-11) wi ...022329749
draft genome sequence of bacillus thuringiensis 147, a brazilian strain with high insecticidal activity.bacillus thuringiensis is a ubiquitous gram-positive and sporulating bacterium. its crystals and secreted toxins are useful tools against larvae of diverse insect orders and, as a consequence, an alternative to recalcitrant chemical insecticides. we report here the draft genome sequence of b. thuringiensis147, a strain isolated from brazil and with high insecticidal activity. the assembled genome contained 6,167,994 bp and was distributed in seven replicons (a chromosome and 6 plasmids). we iden ...026517667
characterization of cry2-type genes of bacillus thuringiensis strains from soilisolated of sichuan basin, china.sichuan basin, situated in the west of china, is the fourth biggest basin in china. in order to describe a systematic study of the cry2-type genes resources from bacillus thuringiensis strains of sichuan basin, a total of 791 bacillus thuringiensis strains have been screened from 2650 soil samples in different ecological regions. the method of pcr-restriction fragment length polymorphism (pcr-rflp) was used to identify the type of cry2 genes. the results showed that 322 bacillus thuringiensis st ...024031615
monitoring resistance to bacillus thuringiensis subsp. israelensis in the field by performing bioassays with each cry toxin separately.bacillus thuringiensis subsp. israelensis (bti) is increasingly used worldwide for mosquito control and is the only larvicide used in the french rhône-alpes region since decades. the artificial selection of mosquitoes with field-persistent bti collected in breeding sites from this region led to a moderate level of resistance to bti, but to relatively high levels of resistance to individual bti cry toxins. based on this observation, we developed a bioassay procedure using each bti cry toxin separ ...024037105
bacillus thuringiensis cry1a toxins are versatile proteins with multiple modes of action: two distinct pre-pores are involved in toxicity.cry proteins from bacillus thuringiensis are insecticidal pfts (pore-forming toxins). in the present study, we show that two distinct functional pre-pores of cry1ab are formed after binding of the protoxin or the protease-activated toxin to the cadherin receptor, but before membrane insertion. both pre-pores actively induce pore formation, although with different characteristics, and contribute to the insecticidal activity. we also analysed the oligomerization of the mutant cry1abmod protein. th ...024456341
functional characterizations of residues arg-158 and tyr-170 of the mosquito-larvicidal bacillus thuringiensis cry4ba.the insecticidal activity of bacillus thuringiensis (bt) cry toxins involves toxin stabilization, oligomerization, passage across the peritrophic membrane (pm), binding to midgut receptors and pore-formation. the residues arg-158 and tyr-170 have been shown to be crucial for the toxicity of bt cry4ba. we characterized the biological function of these residues. in mosquito larvae, the mutants r158a/e/q (r158) could hardly penetrate the pm due to a significantly reduced ability to alter pm permeab ...024286331
essential oils from two eucalyptus from tunisia and their insecticidal action on orgyia trigotephras (lepidotera, lymantriidae).essential oils extracted from aromatic and medicinal plants have many biological properties and are therefore an alternative to the use of synthetic products. the chemical composition of essential oils from two medicinal plants (eucalyptus globulus and e. lehmannii) was determined and, their insecticidal effects on the third and fourth larval stages of orgyia trigotephras were assessed.025204015
isoleucine at position 150 of cyt2aa toxin from bacillus thuringiensis plays an important role during membrane binding and oligomerization.cyt2aa2 is a mosquito larvicidal and cytolytic toxin produced by bacillus thuringiensis subsp. darmstadiensis. the toxin becomes inactive when isoleucine at position 150 was replaced by alanine. to investigate the functional role of this position, ile150 was substituted with leu, phe, glu and lys. all mutant proteins were produced at high level, solubilized in carbonate buffer and yielded protease activated product similar to those of the wild type. intrinsic fluorescence spectra analysis sugges ...023527862
toxicity studies for indigenous bacillus thuringiensis isolates from malang city, east java on aedes aegypti larvae.to investigate the toxicity of indigenous bacillus thuringiensis (b. thuringiensis)isolates from malang city for controlling aedes aegypti (ae. aegypti) larvae.023593589
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