Publications

assessment of the conduction of the cardiac impulse by a new epicardiac surface and stimulation technique (sst-ecg) in langendorff perfused mammalian hearts.	the assessment of the effects on the conduction of the cardiac impulse and of refractoriness simultaneously from multiple cardiac structures is important to evaluate the mode of action of new compounds, as well as to investigate undesired cardiac side effects. the measurements of intracardiac electrical activities of special structures have required catheter methods or averaging techniques to produce clear deflections of low-level potential wave forms. we have developed an epicardial surface ele ...	1989	2724989
neuropeptide y afferents have synaptic interactions with histaminergic (histidine decarboxylase-immunoreactive) neurons in the rat brain.	the synaptic interaction was identified between histaminergic cells and neuropeptide y (npy)-immunoreactive axon terminals in the caudal magnocellular nucleus, by means of the immunoelectron microscopic mirror method. this observation suggests that npy afferents exert monosynaptic influences upon the functioning of the central histaminergic neuronal system.	1989	2725952
human basic fibroblast growth factor gene encodes four polypeptides: three initiate translation from non-aug codons.	human basic fibroblast growth factor (bfgf) is an angiogenic polypeptide mitogen present in a wide variety of mesoderm- and neuroectoderm-derived tissues. bfgf cdna and genomic clones predict a 17.8-kda (155-amino acid) gene product based on the presence of a single putative translational initiator atg codon. however, a bfgf protein isolated from human placenta contains two additional amino acids nh2-terminal to the predicted initiator methionine. we report here that the human cell line sk-hep-1 ...	1989	2726761
large phosphate shifts with treatment for hyperglycemia.	although hypophosphatemia is known to commonly accompany therapy for hyperglycemia, it is not generally appreciated that severe life-threatening depletion of phosphate may occur. i followed up two patients who had precipitous drops during intravenous insulin therapy for diabetic ketoacidosis and hyperosmolar nonketotic state. the patient with diabetic ketoacidosis had a phosphate level nadir that equaled the lowest recorded phosphate level in a living human (0.03 mmol/l). because (1) serum phosp ...	1989	2730261
hypoxic pulmonary vasoconstrictor response with asymmetric oleic acid injury in the dog.	the hypoxic pulmonary vasoconstrictive (hpv) response is characterized by two components: intrapulmonary blood flow redistribution and pulmonary artery pressure (pap) augmentation. whether both remain intact after oleic acid lung injury has not been assessed. in an asymmetric model of right lung oleic acid edema, we examined the relative magnitude of each component on the hpv response to right lung inspired hypoxia. before oleic acid administration, right lung hypoxia decreased perfusion to the ...	1989	2736926
the practice of euthanasia in the netherlands.		1989	2737857
biological properties of streptonigrin derivatives. iii. in vitro and in vivo antiviral and antitumor activities.	antitumor antibiotic streptonigrin (stn-cooh) is a potent inhibitor of avian myeloblastosis virus (amv) and human immunodeficiency virus reverse transcriptases. the carboxyl group at 2'-position of stn-cooh was modified to give esters, hydrazide, amides and amino acid derivatives for biological studies. against amv reverse transcriptase, the hydrazide, amides and amino acid derivatives showed inhibitory activity, which compared favorably to that of stn-cooh, with the id50 values ranging 2-8 micr ...	1989	2737955
leucine-zipper motif update.		1989	2739731
endogenous serum albumin content in brain after short-lasting epileptic seizures.	epileptic seizures can transiently alter the blood-brain barrier. we have determined the content of extravasated endogenous serum albumin in the brain and its change with time after bicuculline (0.3 mg/kg) induced epileptic seizures of a few minutes' duration in conscious rats. the brains were perfused with saline in situ 5 min, 2 h, 24 h, 3 or 7 days after the injection of bicuculline. the content of endogenous serum albumin in the cerebral cortex, diencephalon, mesencephalon, pons and cerebell ...	1989	2743155
glycaemic effects of traditional european plant treatments for diabetes. studies in normal and streptozotocin diabetic mice.	twelve plants used for the traditional treatment of diabetes mellitus in northern europe were studied using normal and streptozotocin diabetic mice to evaluate effects on glucose homeostasis. the plants were administered in the diet (6.25% by weight) and/or as decoctions or infusions in place of drinking water, to coincide with the traditional method of preparation. treatment for 28 days with preparations of burdock (arctium lappa), cashew (anacardium occidentale), dandelion (taraxacum officinal ...	1989	2743711
[surgical proposals concerning omental mesh graft. experimental studies].	in general surgery, the advantages deriving from the utilisation of omental graft are attributable to the ease with which it takes, its regenerating potential and the modulator action on tissue repair. personal experience regarding the peritoneization and covering of surgical liver surfaces is reported and the use in various sectors of surgery of omental mesh graft is recommended.	1989	2747948
heterogeneity of anti-u demonstrable by the use of papain-treated red cells.	when red cells (rbcs) are treated with papain, one form of the u antigen, which we have named ups (u papain-sensitive), is almost completely removed or denatured. a second form, upr (u papain-resistant), remains unaltered on the treated rbcs. tests on 42 examples of anti-u showed that two contained only anti-ups, 19 contained only -upr, and 21 contained separable -ups and -upr. in those sera containing both antibodies, anti-upr was always the stronger of the two. these findings suggest 1) that u ...	1989	2749873
morphology of the bovine sertoli cell during the spermatogenetic cycle.	the morphology of the bovine sertoli cell was studied during 6 different phases of the spermatogenetic cycle. tubular dimensions do not vary significantly during the phases. sertoli cells occupy 27.0% (phase 4) to 38.4% (phase 8) of the tubular epithelium. sertoli cells of phase 1 are approximately 20% larger than during the other phases. 30-35% of sertoli cell volume consists of organelles. mitochondrial (about 5.0%) and nuclear (about 5.7%) volume densities remain remarkably stable during the ...	1989	2752416
enhancement of radiation-induced dna double-strand breaks and micronuclei in human colon carcinoma cells by n-methylformamide.	the differentiation-inducing agent n-methylformamide (nmf) enhances the sensitivity of some cell lines to ionizing radiation. to elucidate the mechanism of nmf-mediated radiosensitization, we examined the effects of this agent on gamma-ray-induced dna double-strand breaks and micronuclei in two cell lines, clone a (human colon carcinoma) and hca-1 (murine hepatocarcinoma). both cell lines form a better differentiated phenotype upon exposure to nmf, yet only clone a is radiosensitized. the neutra ...	1989	2756107
perioral anesthesia disrupts maternal behavior during early lactation in long-evans rats.	the role of perioral somatosensory stimulation from pups in maternal behavior was investigated in lactating long-evans rats (rattus norvegicus) during early lactation by local anesthesia of the mystacial pads with lidocaine, which blocks conduction in the infraorbital nerve. it was concluded that (1) acute perioral anesthesia markedly inhibited pup retrieval when the subjects had no formal pretreatment retrieval experience. further, these dams displayed little tolerance to the lidocaine treatmen ...	1989	2757582
biological activity in the repopulating rat spermatocyte after the withdrawal of gossypol treatment: ii. the activity for the synthesis of cellular and nuclear basic proteins.	the synthesis of cellular and nuclear basic proteins in the first generation of the repopulated pachytene spermatocyte (rps) and the control pachytene spermatocyte (cps) was studied by the incorporation of 3h-arginine into proteins. after pretreatment with gossypol for various lengths of time, the synthetic activity for cellular protein in the rps was slightly inhibited. in comparison to cps, on the other hand, the inhibition ranged from 12% to 26% (for gossypol pretreatment from 3 to 12 weeks, ...	1989	2758844
relationship between concentration and anticonvulsant effect of phenytoin against electroshock-induced seizures in rats: comparison of sampling sites for concentration determinations.	the purpose of this investigation was to determine the optimum sampling site for phenytoin concentration measurements in the context of pharmacodynamic studies of the anticonvulsant effect of phenytoin. determination of drug concentrations in the serum, serum water, brain, and cerebrospinal fluid (csf) of rats as a function of time after iv injection of a 6-mg/kg dose revealed a significant disequilibrium between brain and serum water for 15 min and between csf and serum water for 5 min after in ...	1989	2760817
protein synthesis and degradation in non-cultured and in-vitro cultured rabbit blastocysts.	in 'pulse-chase' experiments synthesis and half-lives of leucine-labelled proteins were determined in rabbit blastocysts. embryos were either non-cultured controls or were cultured for 24 h or 48 h in ham's f-10 medium supplemented with homologous serum or uterine flushings. in control blastocysts protein synthesis increased by a factor of 10 between day 4 and day 5. half-lives of newly synthesized proteins were 32 h in day-4 and 99 h in day-5 control blastocysts. in-vitro culture of day-4 blast ...	1989	2760880
growth and metabolic properties of bacteroides intermedius in anaerobic continuous culture.	two strains of bacteroides intermedius bh20/30 and bh18/23, have been grown in anaerobic continuous culture under various conditions for periods up to 54 days. strain bh20/30 grew over a relatively wide ph range from 5-8 with a maximum at ph 7.0 at a dilution rate (d) of 0.1 h-1 with a glucose limitation, while strain bh18/23 had an optimum between 5.8 and 7.3 and would not grow above and below this range. the maximum growth rate (mu max) for the latter strain was shown to be 0.23 h-1, or a doub ...	1989	2762020
molecular cloning and sequence analysis of putative chicken prolactin cdna.	a cdna library was prepared from mrna isolated from anterior pituitary glands of incubating bantam hens, in which prolactin mrna levels were predicted to be very high. nine clones, representing abundant mrna species, were identified and shown to contain homologous sequences. two clones, of 871 bp and 580 bp, were analysed by dna sequencing. the shorter clone was found to be a truncated cdna product but otherwise identical to the longer clone. the 871 bp cdna, prl101, contains an open reading fra ...	1989	2765112
endothelium-dependent inhibition of ergonovine-induced contraction is impaired in porcine coronary arteries with regenerated endothelium.	the inhibitory effects of the endothelium against ergonovine-induced contraction were examined in isolated porcine coronary arteries under normal conditions and after endothelial regeneration. endothelium-dependent responses were examined in vitro in normal yorkshire pigs (n = 16) and in pigs that had undergone balloon endothelium removal of the left anterior descending coronary artery (lad) 4 weeks before the study (n = 10). the presence of a complete endothelial lining was confirmed histologic ...	1989	2766515
smokeless tobacco use among adolescents: correlates and concurrent predictors.	seventh grade students (n = 1539) from three regions of new york state were surveyed to determine the prevalence of smokeless tobacco use and its relationship to seven background variables, 13 substance use variables, and 19 psychosocial variables. significant correlations with smokeless tobacco use were found within each of these variable domains. concurrent predictors for each domain were determined using logistic regression analysis. the resulting three models were combined in a stepwise fash ...	1989	2768485
em autoradiographic study of the projections from the dorsal nucleus of the lateral lemniscus: a possible source of inhibitory inputs to the inferior colliculus.	the fine structure of the projection from the dorsal nucleus of the lateral lemniscus (dnll) to the inferior colliculus is examined in the cat. anterograde axonal transport of 3h-leucine and em autoradiographic techniques are used to label axonal endings from dnll. the primary finding is that axonal endings from dnll contain pleomorphic synaptic vesicles and make symmetrical synaptic contacts. this morphology is associated with inhibitory synapses. the projection from dnll is the source of appro ...	1989	2768557
determination of estradiol 2- and 16-alpha-hydroxylase activities in mcf-7 human breast cancer cells in culture using radiometric analysis.	using a radiometric assay the effects of estradiol upon the activity of estradiol 2- and 16 alpha-hydroxylases in mcf-7 human breast cancer cells in culture were studied. after 5 days of treatment and 36 h of withdrawal, incubation in the presence of either 2- or 16 alpha-tritiated substrate was carried out. estradiol (10 nm) significantly increased 16 alpha-hydroxylase activity (21%, p less than 0.01), while no effects on 2-hydroxylase activity was observed. treatment for 6 weeks caused a major ...	1989	2770303
[cardiovascular risk factors in children and adolescents in fuenlabrada: objectives, features of the population, design, participation, equipment, laboratory technics, and quality control].	fuenlabrada is at the present time the most industrialized town placed at the south are of madrid. this population has some peculiar attractive characteristics from the epidemiologic point of view. perhaps the most important is that the majority of its inhabitants are immigrant from other depressed rural areas of the country, with a low socio-economic and cultural level. the new conditions of life could have changed the life expectation and the causes of morbidity and mortality of this populatio ...	1989	2772364
[chronic pyelonephritis in polycystic kidney].	the characteristics of chronic pyelonephritis are studied in 37 patients out of a total of 53 patients with proved renal polycystosis. a group of 71 patients with chronic pyelonephritis selected at random are used as a control group. the frequency of chronic pyelonephritis among the patients with renal polycystosis is 69.8%. the difference between the mean age of the patients with renal polycystosis and chronic pyelonephritis and the patients with renal polycystosis without chronic pyelonephriti ...	1989	2773465
the mobilization of ferritin iron by liver cytosol. a comparison of xanthine and nadh as reducing substrates.	considerable evidence suggests that the release of iron from ferritin is a reductive process. a role in this process has been proposed for two hepatic enzymes, namely xanthine oxidoreductase and an nadh oxidoreductase. the abilities of xanthine and nadh to serve as a source of reducing power for the enzyme-mediated release of ferritin iron (ferrireductase activity) were compared with turkey liver and rat liver homogenates. the maximal velocity (vmax.) for the reaction with nadh was 50 times grea ...	1989	2775199
omega-hydroxyacid derivatives in the epidermis of several mammalian species.	1. omega-hydroxyacids from the acylceramides and acylglucosylceramides of mammalian epidermis were examined by thin-layer and gas-liquid chromatography to determine their degree of unsaturation and chain-length distributions. the species examined included human (homo sapiens), pig (sus scrofa), mouse (mus musculus) and rat (rattus rattus). 2. within a species, the omega-hydroxyacids from the acylceramide and acylglucosylceramide were essentially identical. 3. the human omega-hydroxyacids proved ...	1989	2776425
repair of sublethal damage in two human tumor cell lines grown as multicellular spheroids.	multicellular tumor spheroids (mts) provide a suitable in vitro model to study radiation sensitivity of tumor cells. two cell lines of human origin, obtained from a neuroblastoma (nb-100) and a squamous cell carcinoma (hn-1), were exposed to graded doses (4-9 gy) of radiation with 18 mv photons. radiation was applied either as a single or as a split dose with an interval of 6 hr to determine the extent of sublethal damage repair. treated spheroids regrew at approximately the same growth rate as ...	1989	2777647
the sz mutant hamster: a genetic model of epilepsy or of paroxysmal dystonia?	attacks of sustained dystonic postures of limbs and trunk can be initiated by mild environmental stimuli in an inbred line of syrian hamsters. the trait is determined by an autosomal simple recessive genetic mutation, originally designated by the gene symbol sz, because the abnormal movements were thought to represent epileptic seizures. the attacks, which can be reproducibly initiated by placing the sz mutant hamsters in a new environment, begin with rapid twitches of the vibrissae, flattened e ...	1989	2779593
opening remarks of president fernando e. agrait on the occasion of the 50th anniversary of the cayo santiago macaque colony.		1989	2780954
[toxoplasma gondii infections and oocyst shedding in domestic cats and the significance of this for the epidemiology and epizootiology of toxoplasmosis].	because of the dissemination of oocysts the cat and other felines have a central position within toxoplasma gondii epidemiology and epizootiology. from 1984 to 1986, 267 cats between 2 months and 15 years old were serologically examined (sft) for toxoplasma antibodies. the rate of infection was 59.9 per cent. the infection rate of stray cats (72.5%) was significantly higher than that of domestic cats (40%). the high infection rate of herbivores results from behaviour of stray cats. in faeces of ...	1989	2782669
neurochemical profile of moclobemide, a short-acting and reversible inhibitor of monoamine oxidase type a.	moclobemide belongs to a new generation of short-acting, reversible, monoamine oxidase (mao) inhibitors. in vitro (rat brain homogenates) moclobemide inhibits mao-a selectively with lower potency than many of the reference mao inhibitors. however, when measured ex vivo in the rat, the potency of moclobemide is similar to that of reference compounds. in vivo the drug induces a dose-dependent, short-lasting (8-16 hr) and preferential inhibition of mao-a in the brain and both mao-a and mao-b inhibi ...	1989	2783611
neurochemical profile of moclobemide, a short-acting and reversible inhibitor of monoamine oxidase type a.	moclobemide belongs to a new generation of short-acting, reversible, monoamine oxidase (mao) inhibitors. in vitro (rat brain homogenates) moclobemide inhibits mao-a selectively with lower potency than many of the reference mao inhibitors. however, when measured ex vivo in the rat, the potency of moclobemide is similar to that of reference compounds. in vivo the drug induces a dose-dependent, short-lasting (8-16 hr) and preferential inhibition of mao-a in the brain and both mao-a and mao-b inhibi ...	1989	2783611
a novel calcium signalling response in the breast cancer cell line mda-468.	in the human breast carcinoma cell line mda-468 addition of epidermal growth factor (egf) is growth inhibitory. calcium signalling was investigated in this cell line using the calcium sensitive fluorescent probe indo-1. addition of egf to mda-468 cells resulted in a novel biphasic calcium response. in the first phase of the response egf raised calcium to levels significantly above basal. this was followed by a prolonged fall in calcium to levels significantly lower than original basal levels. th ...	1989	2784677
results of the flow cytometry actg quality control program: analysis and findings.	the aids clinical trial group's (actg) immunology committee was charged with initiating a quality control program for all laboratories participating in the actg program reporting flow cytometry data. forty-one laboratories were evaluated. this report defines the goals of this program and the subsequent findings after 19 send-outs were made. both hiv positive volunteer donors and normal age-matched donors were used. sample sets included both heparin and edta anticoagulated bloods. laboratories we ...	1989	2785890
co-localization of corticotropin-releasing factor- and enkephalin-like immunoreactivities in nerve cells of the rat hypothalamus and adjacent areas.	the co-localization of corticotropin-releasing factor (crf)- and enkephalin (enk)-like immunoreactivities in nerve cells of the rat hypothalamus and adjacent areas was investigated by the simultaneous application of immuno-beta-galactosidase staining and the peroxidase-antiperoxidase (pap) method to the same sections. crf-like immunoreactive cells were stained blue with immuno-beta-galactosidase staining and enk-like immunoreactive cells brown with the pap method. double-labeled cells with overl ...	1989	2786446
protection induced against acute bronchitis--the use of human and rat models to determine mechanisms of action of oral immunization with haemophilus influenzae.		1989	2786462
human t lymphocytes possess a limited in vitro life span.	the t lymphocyte offers certain theoretical advantages over other available cell types for the study of aging. immunosenescence is a well-established part of, and may be directly relevant to, mammalian aging, and the t lymphocyte is well-characterized as to function, cell-surface antigen make-up, and other factors. however, prior efforts at studying in vitro aging of t cells have been hampered by poor reproducibility in doubling potential and the occurrence of a peculiar type of crisis. we have ...	1989	2786475
lymphokine-activated killer (lak) cells inhibit the clonogenic growth of human leukemic stem cells.	the effect of lymphokine-activated killer (lak) cells on the in vitro clonogenic capacity of acute myeloid leukemia (aml) blasts was investigated in a semisolid medium assay. the leukemic clonogenic capacity of 11 aml cases, selected on the basis of their ability to grow in vitro, was highly reduced following overnight preincubation with lak effectors. the degree of colony inhibition, which ranged between 66% and 98% (mean 83.8% +/- 11.4 sd), was quantitatively greater than by 51cr release, whic ...	1989	2786477
acute pathological effects on rats of orally administered wortmannin-containing preparations and purified wortmannin from fusarium oxysporum.	the pathological effects in rats of orally administered wortmannin, a mycotoxin produced by fusarium oxysporum, were studied. weanling rats were fed a wortmannin-containing fungal culture for 4 or 5 days, or were given by intragastric gavage a single, lethal dose of extracts of a wortmannin-containing fungal culture or pure toxin. haemoglobinuria, necrosis of lymphoid tissues and death occurred in rats fed the fungal culture. administration by gavage of extracts of the wortmannin-containing cult ...	1989	2786490
longitudinal study of the in vivo hprt mutant frequency in human t-lymphocytes as determined by a cell cloning assay.	the in vivo frequency of mutants resulting from mutation at the hprt locus in human t-lymphocytes can be determined by a cloning assay. this assay quantifies the frequency of 6-thioguanine-resistant (tgr) t-cells through growth of colonies in 96-well microtiter dishes. the reproducibility of the tgr mutant frequency values has now been assessed in a longitudinal study of six individuals (three male, three female, aged 22-33 years) employing 4-5 blood samples over a 26-37 week time period. clonin ...	1989	2786809
inhibition of enteropathogenic bacteria by human milk whey in vitro.	diarrhea caused by enteropathogenic bacteria is a leading cause of childhood mortality world-wide, particularly in less developed regions. breast-feeding has been advocated to protect infants and children from infectious illnesses. we examined the antibacterial activity of human whey in vitro against multiple strains of the following species of enteropathogenic bacteria, campylobacter jejuni, escherichia coli, salmonella typhimurium, shigella flexneri, shigella sonnei and vibrio cholerae, all is ...	1989	2787902
variceal hemorrhage as a consequence of spontaneous rupture of a pancreatic pseudocyst into the splenic vein.	we describe a case of a 68-yr-old man who died as a consequence of a massive bleed from esophageal varices. at autopsy, a pancreatic pseudocyst that communicated directly with the splenic vein was found. histology confirmed that material from the pseudocyst had embolized to the peripheral portal veins, occluding many of them.	1989	2788993
interleukin 1 in rheumatoid arthritis: potentiation of immune responses within the joint.	specific immunoassays were used to measure il-1 peptides in the serum and synovial fluid of patients with rheumatoid arthritis (ra) and in the serum of age-matched healthy controls. patients with ra had raised levels of both il-1 beta and il-1 alpha in their sera compared to controls. synovial fluid levels of il-1 beta significantly correlated with immunoreactive il-2 and soluble il-2 receptor (sil-2r). in addition, incubation of synovial fluid mnc with human recombinant (hr) il-1 caused a dose- ...	1989	2789325
estradiol-stimulated increases in uterine eosinophils and nuclear type ii estrogen-binding sites are prevented by pertussis toxin.	previous studies from several laboratories have demonstrated that estradiol treatment resulted in an increase in nuclear type ii binding sites. our previous data suggest that this increase was due to the estradiol-stimulated influx of circulating eosinophils. therefore, we suggested that the uterine nuclear type ii estrogen-binding sites were not of uterine origin. in this report we present further evidence to support this hypothesis. treatment of immature rats with estradiol resulted in the sti ...	1989	2792007
effects of soft contacts of differing thickness on corneal wound healing in rabbits.	this study was undertaken to determine the effects of thin (60 microns) and thick (240 microns) soft contact lenses of equal water content (70%) and power on nonlesioned and lesioned rabbit corneas. in one group of animals, corneas were not lesioned. thin lenses were placed on left corneas and thick lenses on right corneas. in a second group, lesions were made in both corneas. left corneas were covered with thin lenses and right corneas with thick lenses. post-treatment times were 8 hr and 24 hr ...	1989	2793355
detoxification of lithocholic acid. elucidation of the pathways of oxidative metabolism in rat liver microsomes.	the hydroxylation of lithocholic acid (3 alpha-hydroxy-5 beta-cholanoic acid) by adult male sprague-dawley rat liver microsomes supplemented with nadph was studied. metabolites were separated by a combination of thin-layer chromatography and high pressure liquid chromatography, both with and without prior methylation and acetylation of the samples. the resulting products were characterized by thin-layer, gas-liquid, and high pressure liquid chromatography by comparison with authentic bile acid s ...	1989	2794781
placental weights of normal and aneuploid early human fetuses.	placental and body weights were plotted for 252 normal human fetuses less than 600 gm and mean regressions and 95% prediction intervals were calculated. placentas from 62 aneuploid fetuses were compared to these standards. placenta to body weight ratios from 34 trisomy-21 fetuses were not reduced but those from 15 trisomy-18 fetuses were generally decreased. one of 5 trisomy-13 ratios was below the prediction interval. among 7 triploid fetuses the ratios were above the normal interval in 3 and b ...	1989	2798268
placental weights of normal and aneuploid early human fetuses.	placental and body weights were plotted for 252 normal human fetuses less than 600 gm and mean regressions and 95% prediction intervals were calculated. placentas from 62 aneuploid fetuses were compared to these standards. placenta to body weight ratios from 34 trisomy-21 fetuses were not reduced but those from 15 trisomy-18 fetuses were generally decreased. one of 5 trisomy-13 ratios was below the prediction interval. among 7 triploid fetuses the ratios were above the normal interval in 3 and b ...	1989	2798268
regional distribution and behavior of the subcutaneous tissue concerning selection and indication for liposuction.	the distribution of the fat tissue in the subcutaneous layer is described. since it has specific characteristics in each region of the human body, careful evaluation of the distribution before selecting patients for surgery is necessary. the subcutaneous tissue after an operation frequently demonstrates a thick and hard fibrosis which is evidence that liposuction should be performed in the lamellar layers. this type of fibrotic tissue in the subcutaneous areola layer often brings on irregulariti ...	1989	2801296
regional distribution and behavior of the subcutaneous tissue concerning selection and indication for liposuction.	the distribution of the fat tissue in the subcutaneous layer is described. since it has specific characteristics in each region of the human body, careful evaluation of the distribution before selecting patients for surgery is necessary. the subcutaneous tissue after an operation frequently demonstrates a thick and hard fibrosis which is evidence that liposuction should be performed in the lamellar layers. this type of fibrotic tissue in the subcutaneous areola layer often brings on irregulariti ...	1989	2801296
presence of cytoadhesins (iib-iiia-like glycoproteins) on human metastatic melanomas but not on benign melanocytes.	glycoproteins iib and iiia, a heterodimer complex, play a vital role in blood platelet aggregation and are members of a wide family of membrane receptors known as integrins or cytoadhesins. cellular interaction to extracellular matrix (ecm) adhesive proteins is mediated by integrins. certain tumor cells are known to interact with ecm and blood platelets in the process of metastasis. however, it is not known if tumor cells, compared with their normal counterparts, acquire iib-iiia-like receptors ...	1989	2801613
prevention of rectal cancer induced by n-nitrosobis(2-oxopropyl)-amine by exogenous testosterone in mrc rats.	rectal carcinogenicity of n-nitrosobis(2-oxopropyl)amine (bop) in male mrc wistar rats was shown to be inhibited by exogenous testosterone (t) when the hormone was given during, but not after, administration of the carcinogen. this effect was independent of the dose and frequency of bop, which was given either weekly for 20 weeks orally or daily for 3 days subcutaneously. since, except for prostatic cancer, the incidence and the patterns of other bop-induced tumors were not altered by t, this ho ...	1989	2805225
prevention of rectal cancer induced by n-nitrosobis(2-oxopropyl)-amine by exogenous testosterone in mrc rats.	rectal carcinogenicity of n-nitrosobis(2-oxopropyl)amine (bop) in male mrc wistar rats was shown to be inhibited by exogenous testosterone (t) when the hormone was given during, but not after, administration of the carcinogen. this effect was independent of the dose and frequency of bop, which was given either weekly for 20 weeks orally or daily for 3 days subcutaneously. since, except for prostatic cancer, the incidence and the patterns of other bop-induced tumors were not altered by t, this ho ...	1989	2805225
purification of a vasodilator-regulated phosphoprotein from human platelets.	cyclic-nucleotide-elevating vasodilators such as prostaglandin e1, prostacyclin, sodium nitroprusside and endothelium-derived relaxing factor inhibit both contraction of vascular smooth muscle cells and the aggregation of platelets at an early step of the activation cascade. previous studies from this laboratory [waldmann, r., nieberding, m. and walter, u. (1987) eur. j. biochem. 167, 441-448) established that in human platelets cyclic-nucleotide-elevating vasodilators stimulated a pattern of pr ...	1989	2806262
differential inhibition of downstream gene expression by the cauliflower mosaic virus 35s rna leader.	the effect of the 600 nucleotide-long camv 35s rna 5' leader sequence on the expression of downstream genes was analyzed both in plant protoplasts and in vitro. for transient expression studies in protoplasts derived from host and nonhost plants, the bacterial chloramphenicol acetyl transferase (cat) gene was fused to the initiation codon of orf vii. the leader sequence reduced cat expression two- to four-fold in protoplasts derived from three host species, but 10- to 50-fold in protoplasts deri ...	1989	2815595
differential inhibition of downstream gene expression by the cauliflower mosaic virus 35s rna leader.	the effect of the 600 nucleotide-long camv 35s rna 5' leader sequence on the expression of downstream genes was analyzed both in plant protoplasts and in vitro. for transient expression studies in protoplasts derived from host and nonhost plants, the bacterial chloramphenicol acetyl transferase (cat) gene was fused to the initiation codon of orf vii. the leader sequence reduced cat expression two- to four-fold in protoplasts derived from three host species, but 10- to 50-fold in protoplasts deri ...	1989	2815595
the measurement of secretory endometrial protein pp14 in serum from postmenopausal women receiving unopposed estrogen or continuously combined estrogen/progestogen.	a highly sensitive radioimmunoassay was developed to measure the low serum concentrations of the endometrial secretory placenta protein 14 (pp14) in postmenopausal women. the assay was established by selecting optimum reaction conditions for radioactive labelling of pp14, combined with simple procedures for the purification of the labelled pp14. the pp14 assay was used in 2 groups of healthy, early postmenopausal women blindly receiving either estrogen monotherapy (n = 20, placebo n = 25) or con ...	1989	2816479
on the estimation of pulmonary capillary pressure from arterial occlusion.	we have attempted to evaluate some approaches for estimating pulmonary capillary pressure from the transient pressure data obtained from the tip of the swanz-ganz catheter after inflation of the balloon. to this end experiments were carried out in anesthetized dogs in which pulmonary vasoconstriction was induced by infusion of histamine, serotonin, or norepinephrine or by hypoxia. in a group of closed chest dogs, the time course for the fall in the catheter tip pressure toward the wedge pressure ...	1989	2817586
[visualization of forensically important foreign bodies by direct roentgen magnification].	the detection of foreign bodies of microscopic dimensions with radiographic techniques is limited by the size and density of the particle and the radiographic resolution. these restrictions can be of decisive value in case work. the high grade direct radiographic magnification technique was successfully used in preceding experiments. due to its 1 mu focal spot, this tube allows direct magnifications up to 250 times. in present studies, the advantages as well as the limitations of this new techni ...	1989	2818477
[visualization of forensically important foreign bodies by direct roentgen magnification].	the detection of foreign bodies of microscopic dimensions with radiographic techniques is limited by the size and density of the particle and the radiographic resolution. these restrictions can be of decisive value in case work. the high grade direct radiographic magnification technique was successfully used in preceding experiments. due to its 1 mu focal spot, this tube allows direct magnifications up to 250 times. in present studies, the advantages as well as the limitations of this new techni ...	1989	2818477
bacterial diarrhoea and treatment.		1988	2893218
sex as a risk factor for htlv-i spread among intravenous drug abusers.		1988	2895250
relationship of free intracellular calcium to the cytolytic activity of entamoeba histolytica.	entamoeba histolytica adherence and destruction of host cells is required for in vivo pathogenicity; amebic in vitro adherence is mediated by a galactose- or n-acetyl-d-galactosamine-inhibitable surface lectin (gal/galnac adherence lectin). free intracellular ca2+ concentration [( ca2+]i) was measured in living amebae and target cells during amebic cytolysis of chinese hamster ovary (cho) cells and human polymorphonuclear neutrophils by utilizing the ca2+ probe fura-2 and computer-enhanced digit ...	1988	2897335
antimicrobial susceptibility of bordetella pertussis (part i).	in this review of the literature data are collected from the more recent studies on the susceptibility of bordetella pertussis to penicillins, cephalosporins, other beta-lactam antibiotics, aminoglycosides, tetracyclines, erythromycin, josamycin, co-trimoxazole and various other antibiotics. the methods of susceptibility testing of b. pertussis are discussed and suggestions for standardization are made.	1988	2897337
the high molecular weight receptor to transforming growth factor-beta contains glycosaminoglycan chains.	proteoglycans are constituents of the cell surface that may play important roles in the regulation of cell behavior. here we report that the 250-kda receptor subunit that binds the multifunctional protein, transforming growth factor-beta 1 (tgf-beta 1), contains chains of heparan sulfate and chondroitin sulfate and thus is a proteoglycan. digestion of tgf-beta 1-receptor complexes with glycosaminoglycan (gag)-specific degradative enzymes yield core proteins of 115-140 kda. cell monolayers that h ...	1988	2897367
a selective inhibitor of camp-specific phosphodiesterase, ro 20-1724, has no effect on the quantal release of acetylcholine from the mouse phrenic nerve.	the indirect twitch response of the mouse isolated phrenic nerve-diaphragm preparation, partially paralysed by tubocurarine, was restored only by about 10% by ro 20-1724 at 2 to 280 microm. the solvent vehicle, dimethylsulphoxide, also showed the same effect to a similar extent. intracellular recordings with glass microelectrodes revealed that ro 20-1724 (40 microm) affected neither the resting membrane potential, the amplitude and frequency of miniature endplate potentials nor the amplitude of ...	1988	2897454
apa i and sst i rflps at the insulin-like growth factor ii (igf2) locus on chromosome 11.		1988	2897668
human herpesvirus 6 and the henle-koch postulates.		1988	2901654
interaction between anti-influenza viral polymerase antibodies and rnp particles using the in vitro transcription process and an immunogold labelling technique.	immunogold labelling and in vitro transcription of influenza virus vrna have been used to analyse the interaction of anti-influenza polymerase antibodies with influenza-ribonucleoprotein (rnp) complexes. the polymerase proteins (p proteins) were localized exclusively at one end of the rnp segments. in the course of transcription the amount of p protein decreased significantly. the in vitro transcriptase activity y of influenza a virus rnp complexes in the presence of anti-polymerase antibodies t ...	1988	2904734
blockade of spinal pathways decreases pre- and postganglionic discharge differentially.	although discharge of many sympathetic nerves decreases substantially on transection of the spinal cord in cats, firing of splenic and mesenteric postganglionic nerves continues unabated (r. l. meckler and l. c. weaver, brain res. 338: 123-135, 1985; r. d. stein and l. c. weaver. j. physiol. lond. 396: 155-172, 1988). therefore, ongoing sympathetic outflow directed to the splanchnic circulation was proposed to be less dependent on supraspinal excitatory drive than that directed to other vascular ...	1988	2904774
pharmacological properties of the positive inotropic and alpha 1-adrenoceptor blocking agent saterinone.	the pharmacological properties of saterinone [+/-)-1,2-dihydro-5-[4-[2-hydroxy-3-[4-(2-methoxyphenyl)-1-piperazinyl] propoxy]phenyl]-6-methyl-2-oxo-3-pyridine-carbonitrile, bdf 8634) were investigated in isolated organs of the guinea pig and in human platelets. saterinone was found to be a potent antagonist at vascular alpha 1-adrenoceptors with a pa2-value of 8.46 +/- 0.12. besides its affinity for alpha 1-adrenoceptors saterinone exerted a positive inotropic effect in the isolated papillary mu ...	1988	2906245
[effects of prazosin, sgb-1534, dobutamine and isoproterenol on congestive heart failure in dogs].	effects of prazosin, dobutamine, isoproterenol and sgb-1534, a new alpha-blockade, on congestive heart failure (chf) in dogs were investigated. the model was made by protease injection into the left ventricular free wall, saline loading, and dextran and methoxamine infusions. by this maneuver, left atrial pressure (lap), systemic vascular resistance (svr) and left ventricular end-diastolic pressure (lvedp) were markedly increased, aortic blood flow (aobf) was decreased, and systemic blood pressu ...	1988	2906315
malaria as anthropo-ecosystem. part i: general concept.		1988	2906647
feeding by mucin and intestinal growth of some enteric bacterial pathogens.	enteroinvasive escherichia coli, salmonella typhi-murium, shigella sonnei, shigella flexneri, as well as e. coli k-12 show dose dependent growth in minimal medium completed with purified hog gastric "granular mucin". this ability is based on alpha-galactosidase production: defective, melibiose (and galactose) non-fermenting k-12 mutant were unable to utilize mucin. the viability of the parent k-12 strain in the cecal content of mice is significantly higher than that of its mel- mutant phenotype. ...	1988	2469280
[bovine herpesvirus type 2 infection].		1988	2845879
virulence-associated genetic regions comprising 31 kilobases of the 230-kilobase plasmid in shigella flexneri 2a.	by random transposon tn5 insertions, we previously identified six virulence-associated sali fragments, b, d, f, g, h, and p, in the 230-kilobase plasmid pmysh6000 of shigella flexneri 2a. in this study, we analyzed the sites of 134 independent tn5 insertions on four contiguous sali fragments, b, p, h, and d, of pmysh6000 and identified five virulence-associated regions; four were associated with inducing a positive sereny test (ser), invasion into epithelial cells (inv), binding to congo red (pc ...	1988	2836357
molecular basis of sindbis virus neurovirulence in mice.	we examined a variety of strains of sindbis virus for the genetic changes responsible for differences in neurovirulence in mice. sv1a (a low passage of the ar339 strain of sindbis virus), a neuroadapted sindbis virus (nsv), and two laboratory strains of sindbis virus (hrsp and toto1101) were examined. nsv causes severe encephalomyelitis with hind-limb paralysis and high mortality after intracerebral inoculation in weanling mice. in contrast, sv1a causes only mild, nonfatal disease in weanling mi ...	1988	2836615
development of an auxotrophic oral live shigella flexneri vaccine.	an oral live attenuated shigella flexneri vaccine candidate strain was constructed by making it auxotrophic and dependent on aromatic metabolites not available in mammalian tissues. an arod gene of escherichia coli k12 strain nk 5131, inactivated by insertion in it of the tn 10 transposon, was transduced using phage p1 into a virulent s. flexneri serotype y strain (sfl 1) isolated from a patient with bacillary dysentery. one of the transductant strains sfl 114 was found to invade hela cells in v ...	1988	2838986
the structure of the human skin fibroblast collagenase gene.	genomic clones containing the complete gene encoding human fibroblast interstitial collagenase were isolated from a lambda phage human dna library. the gene is comprised from 10 exons and spans 8.2 kilobase pairs. we have mapped the relative positions and determined the dna sequence of all the exon/intron borders of the gene. the organization of the human interstitial collagenase gene is very similar to that of rabbit collagenase and of two other extracellular matrix (ecm) metalloproteases: rat ...	1988	2839503
the structure of the human skin fibroblast collagenase gene.	genomic clones containing the complete gene encoding human fibroblast interstitial collagenase were isolated from a lambda phage human dna library. the gene is comprised from 10 exons and spans 8.2 kilobase pairs. we have mapped the relative positions and determined the dna sequence of all the exon/intron borders of the gene. the organization of the human interstitial collagenase gene is very similar to that of rabbit collagenase and of two other extracellular matrix (ecm) metalloproteases: rat ...	1988	2839503
selective induction of chromosomal gene expression by human cytomegalovirus.	during permissive infection, human cytomegalovirus (hcmv) increases the activities of certain cellular enzymes. in this report, we show that induction of these cellular genes occurs at the level of rna. the abundance of several additional growth-regulated cellular transcripts in hcmv-infected human foreskin fibroblasts (hff) was also compared to that in serum-stimulated hff cells. hcmv infection of hff cells resulted in increased abundance of ornithine decarboxylase, thymidine kinase, heat-shock ...	1988	2842946
detection of leukotriene b4 in cardiac tissue and its role in infarct extension through leucocyte migration.	the main left coronary artery of rats was ligated near its origin under light ether anaesthesia and the infarction observed for 48 h. the ischaemic area was determined after an intravenous injection of pontamine sky blue dye 1 h before induction of cardiac arrest with potassium chloride. the unstained area (true ischaemic area) decreased with time to 27.1% of the left ventricle at 48 h, whereas the intensely stained area between the normal and the true ischaemic areas increased with time, sugges ...	1988	2844405
prenatal diazepam exposure affects beta-adrenergic receptors in brain regions of adult rat offspring.	the postnatal development of [3h]dihydroalprenolol binding to beta-adrenergic receptors has been studied in frontal cortex, cerebellum, striatum, and hypothalamus of the rat after prenatal and perinatal exposure to diazepam. dams were injected subcutaneously with single daily doses of 1 mg of diazepam/kg from day 7 to 20 of gestation or from day 15 of gestation to day 6 after birth. prenatal exposure had no effect on litter size or length of gestation or on the postnatal development of body and ...	1988	2844991
[specificity of the protective action of a ribosomal shigella vaccine and the absence of activity in the ribosomes from r mutants].	the ribosomal preparations of s. sonnei and some other bacterial species were obtained by the method of differential centrifugation, and the specificity of their protective action was studied in the keratoconjunctivitis test on guinea pigs. the ribosomal preparations were introduced parenterally in a single injection, and their protective action was determined two weeks later by the challenge of the animals with s. sonnei virulent strain and the subsequent calculation of the efficiency index (ei ...	1988	2464261
[lipid peroxidation in experimental viral infection and the effect on it of interferons and alpha-tocopherol].	lipid peroxidation as well as effects of various interferons (native, from fibroblasts of new born and adult animals) on the peroxidation were studied in blood plasma, erythrocytes, liver and kidney tissues of mice with viral infection. these interferons, simultaneously with antiviral action, exhibited the positive effect on lipid peroxidation, while the interferon from fibroblasts of newborns produced the side reactions. simultaneous use of the interferon and alpha-tocopherol removed these side ...	1988	2467439
inhibition of allergic encephalomyelitis by the iron chelating agent desferrioxamine: differential effect depending on type of sensitizing encephalitogen.	induction of experimental allergic encephalomyelitis (eae) in lewis rats by injection of guinea pig (gp) spinal cord homogenate (sch) plus adjuvant (sch-cfa) can be inhibited by treatment with the iron chelating agent desferrioxamine (dfom). interestingly, induction of eae with purified myelin basic protein (bp-cfa) is not inhibited with dfom. this dichotomy does not appear to be due to any quantitative differences in the two inocula since minimal clinical eae produced by threshold levels of bp ...	1988	2447124
heterogeneity in chromosome damage and repair rates after bleomycin in ataxia telangiectasia cells.	cells derived from patients with ataxia telangiectasia (at) are known to be exceptionally sensitive to ionizing radiation and chemotherapeutic agents such as bleomycin (blm), neocarzinostatin, and etoposide. this increased sensitivity is manifested by high chromosome aberration frequencies after treatment. in order to probe the underlying basis for this phenomenon, the technique of premature chromosome condensation was used to determine whether the increased chromosome damage observed after bleo ...	1988	2446745
development and testing of invasion-associated dna probes for detection of shigella spp. and enteroinvasive escherichia coli.	genetic determinants of the invasive phenotype of shigella spp. and enteroinvasive escherichia coli (eiec), two common agents of bacillary dysentery, are encoded on large (180- to 210 kilobase), nonconjugative plasmids. several plasmid-encoded antigens have been implicated as important bacterial ligands that mediate the attachment and invasion of colonic epithelial cells by the bacteria. selected invasion plasmid antigen (ipa) genes have recently been cloned from shigella flexneri serotype 5 int ...	1988	2830310
three distinct forms of type 2a protein phosphatase in human erythrocyte cytosol.	two type 2a protein phosphatases, phosphatases i (mr = 180,000) and iii (mr = 177,000), were purified to near homogeneity from human erythrocyte cytosol. phosphatase i was composed of alpha (34 kda), beta (63 kda), and delta (74 kda) subunits in a ratio of 1:1:1. phosphatase iii comprised alpha, beta, and gamma (53 kda) subunits in the same ratio. heparin-sepharose column chromatography converted most of phosphatase i and 20% of phosphatase iii into alpha 1 beta 1 which were indistinguishable fr ...	1988	2831201
apolipoprotein e-rich hdl binding to liver plasma membranes in copper-deficient rats.	copper deficiency in rats raises plasma cholesterol concentration while reducing live cholesterol concentration. one consequence of this cholesterol redistribution is the accumulation of a large high-density lipoprotein (hdl) particle rich in apolipoprotein e (apo e). the purpose of this study was to determine, using an in vitro binding assay, if the interaction of apo e-rich hdl with hepatic lipoprotein binding sites may be affected by copper deficiency. male sprague-dawley rats were divided in ...	1988	2831541
analysis and isolation of cytomegalovirus dna by field inversion gel electrophoresis.	high molecular weight human cytomegalovirus (cmv) dna was isolated from agarose-embedded infected human diploid cells by employing field inversion gel electrophoresis. a high yield of cmv dna molecules was obtained within 1 week of infecting the cell culture. labelling of the viral dna with biotin by nick translation enabled the detection of cmv-infected cells in sections of paraffin-embedded human adrenal gland by in situ hybridization.	1988	2832532
analysis and isolation of cytomegalovirus dna by field inversion gel electrophoresis.	high molecular weight human cytomegalovirus (cmv) dna was isolated from agarose-embedded infected human diploid cells by employing field inversion gel electrophoresis. a high yield of cmv dna molecules was obtained within 1 week of infecting the cell culture. labelling of the viral dna with biotin by nick translation enabled the detection of cmv-infected cells in sections of paraffin-embedded human adrenal gland by in situ hybridization.	1988	2832532
viruses in sewage waters during and after a poliomyelitis outbreak and subsequent nationwide oral poliovirus vaccination campaign in finland.	during an outbreak of paralytic poliomyelitis in finland in 1984 and 1985 the widespread circulation of the causative wild-type serotype 3 poliovirus in the population was documented by demonstrating the virus in sewage water specimens in 13 different locations in the greater helsinki district and in 13 other cities or towns all over the country. after the nationwide campaign with oral poliovirus vaccine in 1985, poliovirus serotypes 2 and 3 were readily isolated from sewage waters for up to 2 m ...	1988	2833160
monoclonal antibodies with neutralizing activity segregate isolates of bovine viral diarrhea virus into groups. brief report.	isolates of bovine viral diarrhea (bvd) virus were differentiated by monoclonal antibodies (moabs) reactive with the 56 kd viral polypeptide. patterns of neutralizing activity of the moabs indicate that multiple epitopes are involved in virus neutralization.	1988	2833200
interaction between bambuterol and physostigmine: aspects on cholinesterase inhibition and neuromuscular transmission in the smooth and skeletal muscles of the guinea-pig.	bambuterol, the bis-dimethyl carbamate prodrug of terbutaline, and physostigmine were examined with respect to their ability to interfere with the neuromuscular transmission in an isolated vagus nerve-trachea preparation, a phrenic nerve-diaphragm preparation and the transmurally stimulated extensor digotorum longus (edl) isolated from the guinea-pig. physostigmine increased the contractile response of the trachea to stimulation of the vagus nerve. bambuterol had an opposite effect in this respe ...	1988	2848230
comparisons of the pathogenicity of long and short fibres of chrysotile asbestos in rats.	long-term inhalation and intraperitoneal injection studies were undertaken with laboratory rats treated with a specially prepared short-fibre sample of canadian chrysotile asbestos. this was compared, at an equal mass dose, to dust generated from the same chrysotile batch so as to contain the highest possible number of long fibres. the long-fibre cloud contained roughly five times more fibres greater than 5 micron in length as seen by phase contrast optical microscopy (pcom). for increasing leng ...	1988	2848570
expression of env sequences of the bovine leukemia virus (blv) in the yeast saccharomyces cerevisiae.	dna sequences of the envelope (env) gene of the bovine leukemia virus (blv) were expressed in the yeast saccharomyces cerevisiae. two yeast promoters, the repressible pho5 promoter and the constitutive pgk promoter, were used to construct four expression plasmids comprising either a sequence of the surface antigen gp51 or a (gp51 + gp30) sequence. the expressed heterologous gene products were characterized by western blot analysis and competitive radioimmunoassay. by means of northern blot analy ...	1988	2849260
peripheral neuropathy in elderly cba mice.	a late-onset peripheral neuropathy in a mouse with a wider progressive neurological disorder is morphologically characterized for comparison with late life human neuropathies. preliminary studies indicate the presence of a proximal, predominantly motor, axonopathy in the mouse model.	1988	2849396