Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| listeria monocytogenes in multiple habitats and host populations: review of available data for mathematical modeling. | listeria monocytogenes has the ability to survive and multiply in diverse habitats and to cause infection in a variety of animal species and humans. we evaluated the literature on survival and multiplication within and transmission among multiple host populations and habitats, including man, sewage, general environment (soil, water, and vegetation), silage (fermented plant material), animals (including wild and domestic animals), and food processing plants. the available knowledge on l. monocyto ... | 2006 | 17199514 |
| evaluation of effects of primary and secondary enrichment for the detection of listeria monocytogenes by real-time pcr in retail ground chicken meat. | a sybr green i based real-time pcr assay with inla-specific oligonucleotide primers was developed for easy and rapid detection of listeria monocytogenes in a model food that usually has a high incidence of contamination with this pathogen. results with pure cultures and artificially contaminated chicken meat samples indicate that the pcr assay was highly specific and sensitive. the melting point analysis of the 160 bp amplified dna fragment was different for l. monocytogenes isolates of the two ... | 2006 | 17199516 |
| [molecular typing of listeria monocytogenes isolated from clinical and food samples]. | listeria monocytogenes is an emergent foodborne pathogen acquired by the ingestion of contaminated food. this bacterium causes a disease called listeriosis, whose mortality rate world wide is around 20% to 30%, reaching up to 80% in cases of neonatal infections. the random amplified polymorphic dna technique allows different isolates to be distinguished and characterized at the molecular level, which can provide useful information about the diversity of this pathogen in colombia. | 2006 | 17176008 |
| use of microbial antagonism to reduce pathogen levels on produce and meat products: a review. | lactic acid bacteria (lab) are often utilized to control food-borne pathogens on produce and on cooked, fermented, or refrigerated meats. most research to date has focused on the inhibition of listeria monocytogenes, escherichia coli o157:h7, salmonella, clostridium botulinum, and spoilage microorganisms. lab are excellent candidates for reducing pathogen levels on foods because they inhibit the growth of these microorganisms through various mechanisms without causing unacceptable sensory change ... | 2006 | 17215892 |
| effect of co2 on the resuscitation of listeria monocytogenes injured by mild inactivation techniques. | 2006 | 17191465 | |
| microbial safety of mayonnaise based salads: a growth/no growth model for listeria monocytogenes as a predictive tool. | 2006 | 17191531 | |
| listeria monocytogenes in a young patient with non hodgkins lymphoma: case report. | listeria monocytogenes is an intracellular food-borne pathogen, widely distributed in the environment, which rarely causes clinical infection in healthy people, but may cause severe disease in immunocompromised patients. a case of listeriosis is certified in an immunocompromised patient, thus confirming this microorganism to be an opportunistic human pathogen. | 2006 | 17166414 |
| [spontaneous bacterial peritonitis caused by listeria in a patient with cirrhosis: case report]. | infections caused by listeria monocytogenes are usually found in cattle and occasionally appear in humans, particularly pregnant women and immunocompromised individuals. peritonitis by listeria monocytogenes is a rare but dangerous condition that must be recognized early, since it requires a specific treatment. we report a 31 year-old male with alcoholic cirrhosis that developed ascites with abdominal pain and fever. the peritoneal fluid culture yielded listeria monocytogenes. the patients was i ... | 2006 | 17171220 |
| characterization of an hmg-coa reductase from listeria monocytogenes that exhibits dual coenzyme specificity. | hmg-coa reductase (hmgr) is an enzyme critical for cellular cholesterol synthesis in mammals and isoprenoid synthesis in certain eubacteria, catalyzing the nad(p)h-dependent reduction of hmg-coa to mevalonate. we have isolated the gene encoding hmg-coa reductase from listeria monocytogenes and expressed the recombinant 6x-his-tagged form in escherichia coli. using nad(p)(h), the enzyme catalyzes hmg-coa reduction approximately 200-fold more efficiently than mevalonate oxidation in vitro. the pur ... | 2006 | 17128979 |
| listeria monocytogenes meningitis and multiple brain abscesses in an immunocompetent host. favorable response to combination linezolid-meropenem treatment. | 2006 | 17129848 | |
| microbiological baseline study of broiler chickens at swedish slaughterhouses. | this 1-year study was conducted to estimate the prevalence and concentrations of pathogenic and indicator bacteria on swedish broiler chickens. a total of 636 chilled carcasses were collected from 10 slaughterhouses and sent to the national food administration for analyses of carcass rinses. no carcasses were positive for salmonella. campylobacter, predominantly campylobacter jejuni, were detected on 15% (by enrichment) or 14% (by direct plating) of the carcasses. with one exception, all samples ... | 2006 | 17186653 |
| international life sciences institute north america listeria monocytogenes strain collection: development of standard listeria monocytogenes strain sets for research and validation studies. | research and development efforts on bacterial foodborne pathogens, including the development of novel detection and subtyping methods, as well as validation studies for intervention strategies can greatly be enhanced through the availability and use of standardized strain collections. these types of strain collections are available for some foodborne pathogens, such as salmonella and escherichia coli. we have developed a standard listeria monocytogenes strain collection that has not been previou ... | 2006 | 17186661 |
| transfer of listeria monocytogenes during slicing of turkey breast, bologna, and salami with simulated kitchen knives. | in response to continued concerns regarding listeria cross-contamination during the slicing of deli meats, a series of specially prepared grade 304 and 316 stainless steel kitchen knife blades was inoculated with a six-strain listeria monocytogenes cocktail (10(8), 10(5), and 10(3) cfu per blade) composed of two weak, two medium, and two strong biofilm-forming strains. the blades were then attached to an instron 5565 electromechanical compression analyzer and used to slice whole chubs of delicat ... | 2006 | 17186662 |
| inhibition and inactivation of listeria monocytogenes and escherichia coli o157:h7 colony biofilms by micellar-encapsulated eugenol and carvacrol. | the antimicrobial efficacy of carvacrol and eugenol, two essential oil compounds, encapsulated in a micellar nonionic surfactant solution on four strains of listeria monocytogenes (scott a, 101, 108, and 310) and four strains of escherichia coli o157:h7 (h1730, e0019, f4546, and 932) growing as colony biofilms was investigated. carvacrol and eugenol were encapsulated in surfynol 485w at concentrations ranging from 0.3 to 0.9% (wt/wt) at a surfactant concentration of 5% (wt/wt). colony biofilms w ... | 2006 | 17186663 |
| irradiation inactivation of listeria monocytogenes in low-fat ground pork at freezing and refrigeration temperatures. | gamma radiation effectively controls listeria monocytogenes in uncooked and in ready-to-eat foods. this study was conducted to determine if gamma radiation could be used to control l. monocytogenes in ground pork. ground pork was contaminated with l. monocytogenes, kept at refrigeration (4 degrees c), chilling (0 degrees c), and freezing (-18 degrees c) temperatures overnight, exposed to gamma radiation and stored at 4 degrees c for 7 days, and at 0 and -18 degrees c for 60 days. following irrad ... | 2006 | 17186664 |
| effect of gamma radiation and oregano essential oil on murein and atp concentration of listeria monocytogenes. | the effects of gamma radiation and of oregano essential oil alone or in combination with radiation on murein composition of listeria monocytogenes and on the intracellular and extracellular concentration of atp were evaluated. the bacterial strain was treated with two radiation doses, 1.2 kgy to induce cell damage and 3.5 kgy to cause cell death. oregano essential oil was used at 0.020 and 0.025% (wt/vol), which is the mic. all treatments had a significant effect (p < or = 0.05) on the murein co ... | 2006 | 17186665 |
| heat inactivation of listeria monocytogenes and salmonella enterica serovar typhi in a typical bologna matrix during an industrial cooking-cooling cycle. | the heat resistance of salmonella enterica serovar typhi pf-724 and listeria monocytogenes 2812 was determined in a commercial bologna batter. the heat inactivation of the two bacterial species was also studied in a semiautomatic pilot smokehouse under cooking conditions that reproduced an industrial bologna process. s. enterica serovar typhi pf-724 was less heat resistant than l. monocytogenes 2812. the d-values (times required to reduce the population by 1 logarithmic cycle) for s. enterica se ... | 2006 | 17186675 |
| role of uvra in the growth and survival of listeria monocytogenes under uv radiation and acid and bile stress. | listeria monocytogenes encounters numerous stresses both in the food environment and during infection of the host. the ability to survive and tolerate bile and low ph conditions, which are two major stresses, is of particular importance for survival within the host. the uvra gene in other bacteria is involved in the repair of acid-induced dna damage and in adaptation to low ph. thus, a uvra in-frame deletion mutant was constructed to identify the role of uvra in the growth and survival of l. mon ... | 2006 | 17186676 |
| survival and growth of foodborne pathogens during cooking and storage of oriental-style rice cakes. | fresh cooked rice cakes for retail sale are typically held at room temperature because refrigeration dramatically reduces their quality. room temperature, high water activity, and a ph of > 4.6 provided an environment conducive to pathogen growth. to date, no studies have been published regarding survival and growth of foodborne pathogens in fresh cooked rice cakes. this study was undertaken to investigate the effect of steam cooking on foodborne pathogens and their subsequent growth in five var ... | 2006 | 17186677 |
| interval mapping of quantitative trait loci for time-to-event data with the proportional hazards mixture cure model. | interval mapping using normal mixture models has been an important tool for analyzing quantitative traits in experimental organisms. when the primary phenotype is time-to-event, it is natural to use survival models such as cox's proportional hazards model instead of normal mixtures to model the phenotype distribution. an extra challenge for modeling time-to-event data is that the underlying population may consist of susceptible and nonsusceptible subjects. in this article, we propose a semiparam ... | 2006 | 17156279 |
| a bifunctional o-glcnac transferase governs flagellar motility through anti-repression. | flagellar motility is an essential mechanism by which bacteria adapt to and survive in diverse environments. although flagella confer an advantage to many bacterial pathogens for colonization during infection, bacterial flagellins also stimulate host innate immune responses. consequently, many bacterial pathogens down-regulate flagella production following initial infection. listeria monocytogenes is a facultative intracellular pathogen that represses transcription of flagellar motility genes at ... | 2006 | 17158746 |
| microflora of minimally processed frozen vegetables sold in gaborone, botswana. | two hundred samples of minimally processed, frozen, and prepacked potato chips, peas, corn, and a variety of combined vegetables from supermarkets in gaborone, botswana, were examined microbiologically. determination of aerobic mesophilic plate count, aerobic psychrotrophic plate count, lactic acid bacteria, yeasts and molds, coliforms, listeria spp., and staphylococcus aureus were done. chips had the lowest mean log values for all of the microorganisms enumerated except yeasts and molds. the me ... | 2006 | 17133799 |
| growth and stress resistance variation in culture broth among listeria monocytogenes strains of various serotypes and origins. | twenty-five listeria monocytogenes strains of various serotypes and sources, including clinical and food isolates associated with the same outbreaks, were characterized and compared based on growth rates and heat and acid death rates. growth was monitored in tryptic soy broth supplemented with 0.6% yeast extract (tsbye) at 4 and 30 degrees c for 32 days and 20 h, respectively. heat and acid stress responses in tsbye heated to 55 degrees c or acidified to ph 3.0 with lactic acid were evaluated fo ... | 2006 | 17133807 |
| quantifying nonthermal inactivation of listeria monocytogenes in european fermented sausages using bacteriocinogenic lactic acid bacteria or their bacteriocins: a case study for risk assessment. | listeria monocytogenes nctc10527 was examined with respect to its nonthermal inactivation kinetics in fermented sausages from four european countries: serbia-montenegro, hungary, croatia, and bosnia-herzegovina. the goal was to quantify the effect of fermentation and ripening conditions on l. monocytogenes with the simultaneous presence or absence of bacteriocin-producing lactic acid bacteria (i.e., lactobacillus sakei). different models were used to fit the experimental data and to calculate th ... | 2006 | 17133808 |
| intragastric inoculation with a cocktail of listeria monocytogenes strains does not potentiate the severity of infection in a/j mice compared to inoculation with the individual strains comprising the cocktail. | although multistrain cocktails of listeria monocytogenes are used in food inoculation experiments, no studies, to our knowledge, have been reported that use these cocktails in an intragastric mouse model. in this study, we used a five-strain l. monocytogenes cocktail consisting of strains scott a, mfs108, 101m, v7, and 310 and a four-strain l. monocytogenes cocktail containing strains scott a, egd, h7738, and f2365. here, we report that intragastric inoculation of anesthetized mice with approxim ... | 2006 | 17133809 |
| effects of microbial inhibitors and modified atmosphere packaging on growth of listeria monocytogenes and salmonella enterica typhimurium and on quality attributes of injected pork chops and sliced cured ham. | this study was designed to determine the inhibitory effects of modified atmosphere packaging (map), both alone and in combination with potassium lactate and sodium diacetate (plsd), on selected pathogens common to pork products. effects of the treatments on product quality also were assessed. the hypothesis was that high-co2 map would increase the effectiveness of plsd for inhibition of pork pathogens. fresh chops from untreated pork loins and loins that were injected with plsd were inoculated w ... | 2006 | 17133810 |
| assessment of environmental factors on listeria monocytogenes scott a inla gene expression by relative quantitative taqman real-time reverse transcriptase pcr. | several virulence factors are involved in listeria monocytogenes pathogenicity. l. monocytogenes internalins, particularly internalin a, are required for bacterial adhesion to and invasion of human intestinal epithelial cells. the expression of internalins is thus related to virulence. identification of conditions involved in regulating the expression of l. monocytogenes virulence factors is essential for developing targeted strategies to control listeriosis incidence and improving therapeutic a ... | 2006 | 17133822 |
| inactivation of the crp/fnr family of regulatory genes in listeria monocytogenes strain f2365 does not alter its heat resistance at 60 degrees c. | a surprising facet of the listeria monocytogenes genome is the presence of 15 genes that code for regulators in the crp/ fnr family and include the virulence regulator prfa. the genes under the transcriptional control of these regulators are currently undetermined, with the exception of some genes controlled by the major virulence regulator prfa. using 12 strains of l. monocytogenes, each with an inserted gene cassette that interrupts and renders nonfunctional a different l. monocytogenes strain ... | 2006 | 17133823 |
| cutting edge: il-12 inversely regulates t-bet and eomesodermin expression during pathogen-induced cd8+ t cell differentiation. | cytokines are critical determinants for specification of lineage-defining transcription factors of cd4+ t cell subsets. little is known, however, about how cytokines regulate expression of t-bet and eomesodermin (eomes) in effector and memory cd8+ t cells. we now report that il-12, a signature of cell-mediated immunity, represses eomes while positively regulating t-bet in effector cd8+ t cells during infection with listeria monocytogenes. after resolution of infection and abatement of il-12 sign ... | 2006 | 17114419 |
| ifn-beta-mediated up-regulation of cd1d in bacteria-infected apcs. | the expression of cd1d molecules is essential for the selection and activation of a unique subset of t cells, invariant nkt cells, which express limited tcr diversity and have been demonstrated to function in both regulatory and antimicrobial immune responses. although it has been reported that the levels of cd1d expression can be modulated during infection, the mechanisms that mediate this effect are poorly defined. in this study, we show that infection of dendritic cells and macrophages both i ... | 2006 | 17114455 |
| indoleamine 2,3-dioxygenase-expressing dendritic cells form suppurative granulomas following listeria monocytogenes infection. | control of pathogens by formation of abscesses and granulomas is a major strategy of the innate immune system, especially when effector mechanisms of adaptive immunity are insufficient. we show in human listeriosis that dcs expressing indoleamine 2,3-dioxygenase (ido), together with macrophages, are major cellular components of suppurative granulomas in vivo. induction of ido by dcs is a cell-autonomous response to listeria monocytogenes infection and was also observed in other granulomatous inf ... | 2006 | 17111046 |
| single nucleotide polymorphism analysis of the enterocin p structural gene of enterococcus faecium strains isolated from nonfermented animal foods. | the bacteriocins produced by two lactic acid bacteria isolated from nonfermented fresh meat and fish, respectively, and exhibiting a remarkable antilisterial activity, were characterized. bacteriocinogenic strains were identified as enterococcus faecium and the maximum bacteriocin production by both strains was detected in the stationary phase of growth. the activity against listeria monocytogenes was maintained in ph range of 3-7 and was stable in both strains after heating at 100 or 121 degree ... | 2006 | 17103378 |
| the effect of lemon, orange and bergamot essential oils and their components on the survival of campylobacter jejuni, escherichia coli o157, listeria monocytogenes, bacillus cereus and staphylococcus aureus in vitro and in food systems. | to investigate the effectiveness of oils and vapours of lemon (citrus limon), sweet orange (citrus sinensis) and bergamot (citrus bergamia) and their components against a number of common foodborne pathogens. | 2006 | 17105553 |
| efficacy of lactic acid against listeria monocytogenes attached to poultry skin during refrigerated storage. | the aim of this study was to evaluate the effect of lactic acid washing on the growth of listeria monocytogenes on poultry legs stored at 4 degrees c for 7 days. | 2006 | 17105564 |
| listeria monocytogenes translocates throughout the digestive tract in asymptomatic sheep. | ruminants are fed forage which is often contaminated with listeria, and frequently shed listeria monocytogenes with their faeces. the present study was designed to localize the sites of infection in the digestive tract concomitant with listeria faecal excretion in a sheep model. ten listeria-free sheep were inoculated per os with a dose of 10(10) c.f.u. of a pathogenic l. monocytogenes strain. listeria received by two of the ten animals were radiolabelled with (111)indium oxine. the disseminatio ... | 2006 | 17108277 |
| brain abscess due to listeria monocytogenes: first case report in thailand. | brain abscess with bacteremia caused by listeria monocytogenes in a young woman with immune thrombocytopenic purpura was reported. the clinical features included fever, headache, and left-side weakness. computed tomography and magnetic resonance imaging of the brain showed a large single abscess at the right frontoparietal area. l. monocytogenes was isolated from a blood culture. the patient promptly received a surgical drainage. because she had a history of penicillin allergy, and the organism ... | 2006 | 17100394 |
| changing pattern of human listeriosis, england and wales, 2001-2004. | microbiologic and epidemiologic data on 1,933 cases of human listeriosis reported in england and wales from 1990 to 2004 were reviewed. a substantial increase in incidence occurred from 2001 to 2004. ten clusters (60 cases), likely to represent common-source outbreaks, were detected. however, these clusters did not account for the upsurge in incidence, which occurred sporadically, predominantly in patients > or =60 years of age with bacteremia and which was independent of sex; regional, seasonal ... | 2006 | 17073084 |
| surveillance for foodborne-disease outbreaks--united states, 1998-2002. | since 1973, cdc has maintained a collaborative surveillance program for collection and periodic reporting of data on the occurrence and causes of foodborne-disease outbreaks (fbdos) in the united states. | 2006 | 17093388 |
| listeria monocytogenes: food-borne pathogen and hygiene indicator. | in the past 25 years, listeria monocytogenes has become increasingly important as a food-associated pathogen. most european union countries have an annual incidence of human listeriosis of between two and ten reported cases per million. because of its high case fatality rate, listeriosis ranks among the most frequent causes of death due to food-borne illness. listeria monocytogenes infections are responsible for the highest hospitalisation rates (91%) amongst known food-borne pathogens and have ... | 2006 | 17094698 |
| listeria monocytogenes peritonitis in cirrhotic patients: first description in brazil. | two cases of spontaneous bacterial peritonitis (sbp) caused by listeria monocytogenes in cirrhotic patients are reported. in one of the cases, the microorganism was isolated from pleural effusion and ascites. sbp is a serious and common complication of patients with ascites caused by hepatic cirrhosis and the culture of the ascitic fluid is an important tool for the diagnosis and for the more appropriate treatment. although a third generation cephalosporin has usually been employed for empiric t ... | 2006 | 17086320 |
| [regulation of nitrogen metabolism in gram-positive bacteria]. | we searched for new members of the tnra and glnr regulons controlling assimilation of nitrogen in gram-positive bacteria. we identified the regulatory signals for these transcription factors with consensuses atgtnawwwwwwwtnacat for glnr and tgtnawwwwwwwtnaca for tnra. we described the structure and found new potential members for the tnra/glnr regulons in bacillus subtilis, b. licheniformis, geobacillus kaustophilus, oceanobacillus iheyensis, for the tnra regulon in b. halodurans and for the gln ... | 2006 | 17086994 |
| genetic analysis of bacteriocin 43 of vancomycin-resistant enterococcus faecium. | a total of 636 vancomycin-resistant enterococcus faecium (vre) isolates obtained between 1994 and 1999 from the medical school hospital of the university of michigan were tested for bacteriocin production. of the 277 (44%) bacteriocinogenic strains, 21 were active against e. faecalis, e. faecium, e. hirae, e. durans, and listeria monocytogenes. of those 21 strains, a representative bacteriocin of strain vre82, designated bacteriocin 43, was found to be encoded on mobilizable plasmid pdt1 (6.2 kb ... | 2006 | 17088377 |
| antimicrobial leucocin analogues with a disulfide bridge replaced by a carbocycle or by noncovalent interactions of allyl glycine residues. | the type iia bacteriocins are antimicrobial peptides isolated from lactic acid bacteria that act as food preservation agents and have nanomolar activity against pathogens such as listeria monocytogenes. previous reports with mutant bacteriocins indicate that the conserved disulfide bridge between cysteine residues 9 and 14 in bacteriocins such as leucocin a (1) is critical for antibiotic properties, which are mediated by target membrane receptor proteins belonging to the mannose phosphotransfera ... | 2006 | 17076487 |
| cd8 t cell recall responses are regulated by the tissue tropism of the memory cell and pathogen. | whether memory cd8 t cells can be reactivated in nonlymphoid tissues is unclear. using mice lacking the spleen, lymph nodes, or both, we show that the secondary t cell response, but not homeostatic maintenance of memory cells, required lymphoid tissue. whereas primary and secondary cd8 t cell responses to vesicular stomatitis virus infection were lymph node dependent, responses to listeria monocytogenes infection were driven primarily in the spleen. memory cell subset reactivation was also regul ... | 2006 | 17082587 |
| cytosolic localization of listeria monocytogenes triggers an early ifn-gamma response by cd8+ t cells that correlates with innate resistance to infection. | ifn-gamma is critical for innate immunity against listeria monocytogenes (l. monocytogenes), and it has long been thought that nk cells are the major source of ifn-gamma during the first few days of infection. however, it was recently shown that a significant number of cd44highcd8+ t cells also secrete ifn-gamma in an ag-independent fashion within 16 h of infection with l. monocytogenes. in this report, we showed that infection with other intracellular pathogens did not trigger this early ifn-ga ... | 2006 | 17082632 |
| a field study of the microbiological quality of fresh produce of domestic and mexican origin. | produce is responsible for an increasingly larger proportion of foodborne disease outbreaks. in particular, the globalization of the food supply may introduce new food safety risks and allow widespread distribution of contaminated food, particularly produce. the objectives of this study were to: (i) compare the overall quality of domestic and mexican produce throughout the packing process; (ii) examine changes in microbiological quality of both domestic and mexican produce at each stage of produ ... | 2006 | 17045687 |
| an important role of tyk2 in apc function of dendritic cells for priming cd8+ t cells producing ifn-gamma. | tyrosine kinase 2 (tyk2) contributes to the signals triggered by il-12 for ifn-gamma production by nk cells and t cells. we found in this study that tyk2-deficient (-/-) mice showed increased susceptibility at the early stage after an i.p. infection with listeria monocytogenes, accompanied by impaired ifn-gamma production. the numbers of both mhc class ib (h2-m3)- or mhc class ia (kb)-restricted cd8+ t cells producing ifn-gamma and exhibiting cytotoxicity were significantly decreased in tyk2-/- ... | 2006 | 17048270 |
| an in vitro cell-culture model demonstrates internalin- and hemolysin-independent translocation of listeria monocytogenes across m cells. | an ability to translocate the mucosal epithelia through m cells provides invasive pathogens with a rapid means of accessing the mucosal lymphoid tissues. in order to determine the role of m cells in listeria monocytogenes infection, we initially assessed colonization of peyer's patch (pp) epithelium in balb/c mice by vibrio cholerae eltor, wild-type l. monocytogenes and an isogenic hemolysin mutant (lo28deltahly). it was observed that both wild-type l. monocytogenes and deltahly showed preferent ... | 2006 | 17049432 |
| community-acquired listeria monocytogenes meningitis in adults. | listeria monocytogenes is the third most common cause of bacterial meningitis. | 2006 | 17051485 |
| listeria monocytogenes induces the expression of retinoic acid-inducible gene-i. | retinoic acid-inducible gene-i (rig-i) is considered to play a role in innate immunity against virus infections. we showed by immunohistochemical study that rig-i expression is upregulated in vivo in hepatic kupffer cells and in splenic reticular cells of mice infected with listeria monocytogenes. both heat-killed l. monocytogenes and live l. monocytogenes induced the expression of rig-i in cultured raw264.7 murine macrophage-like cells in vitro. rig-i may also be involved in innate immunity aga ... | 2006 | 17053317 |
| listeriosis in portugal: an existing but under reported infection. | listeriosis is a rare disease caused by the bacterium listeria monocytogenes, the normal vehicle of which is food. the disease, which is largely confined to its risk groups of pregnant women, the elderly and immunocompromised individuals, has increased in incidence in recent years. in portugal, listeriosis is not a notifiable infection and available data are scarce. the objective of this work was to collate the available information concerning listeriosis in portugal by compiling a retrospective ... | 2006 | 17054782 |
| immunoproteasomes are essential for clearance of listeria monocytogenes in nonlymphoid tissues but not for induction of bacteria-specific cd8+ t cells. | microbial infections induce the replacement of constitutive proteasomes by immunoproteasomes (i-proteasomes). i-proteasomes support efficient generation of mhc class i epitopes and influence immunodominance hierarchies of cd8(+) t cells. recently, the function of i-proteasomes in antimicrobial responses was challenged by showing that the lack of i-proteasomes has no effect on induction and function of lymphocytic choriomeningitis virus-specific cd8(+) t cells. here, we show that infection with l ... | 2006 | 17056553 |
| structure of internalin c from listeria monocytogenes. | the crystal structure of internalin c (inlc) from listeria monocytogenes has been determined at 2.0 a resolution. several observations implicate inlc in infection: inlc has the same transcriptional activator as other virulence genes, it is only present in pathogenic listeria strains and an inlc deletion mutant is significantly less virulent. while the extended concave receptor-binding surfaces of the leucine-rich repeat (lrr) domains of internalins a and b have aromatic clusters involved in rece ... | 2006 | 17057330 |
| rapid actin monomer-insensitive depolymerization of listeria actin comet tails by cofilin, coronin, and aip1. | actin filaments in cells depolymerize rapidly despite the presence of high concentrations of polymerizable g actin. cofilin is recognized as a key regulator that promotes actin depolymerization. in this study, we show that although pure cofilin can disassemble listeria monocytogenes actin comet tails, it cannot efficiently disassemble comet tails in the presence of polymerizable actin. thymus extracts also rapidly disassemble comet tails, and this reaction is more efficient than pure cofilin whe ... | 2006 | 17060499 |
| mixture cure model with an application to interval mapping of quantitative trait loci. | when censored time-to-event data are used to map quantitative trait loci (qtl), the existence of nonsusceptible subjects entails extra challenges. if the heterogeneous susceptibility is ignored or inappropriately handled, we may either fail to detect the responsible genetic factors or find spuriously significant locations. in this article, an interval mapping method based on parametric mixture cure models is proposed, which takes into consideration of nonsusceptible subjects. the proposed model ... | 2006 | 17063400 |
| effect of ozone on the inactivation of yersinia enterocolitica and the reduction of natural flora on potatoes. | fresh vegetables contaminated with yersinia enterocolitica have been implicated in foodborne disease outbreaks. surfaces of vegetables can become contaminated with pathogenic microorganisms through contact with soil, irrigation water, fertilizers, equipment, humans, and animals. one approach to reduce this contamination is to treat fresh produce with sanitizers. in this study, the ability of ozone to inactivate y. enterocolitica inoculated in water and on potato surfaces was evaluated. furthermo ... | 2006 | 17066913 |
| control of listeria monocytogenes in goat's milk and goat's jben by the bacteriocinogenic enterococcus faecium f58 strain. | the bacteriocinogenic enterococcus faecium f58 strain, a natural goat's jben cheese isolate, lacks decarboxylase activity involved in most biogenic amine formation. it was also sensitive to 13 antibiotics assayed and free of virulence and vancomycin resistance genes. the f58 strain reached the stationary phase after 12 h of growth in sterile goat's milk, and the production of enterocin f-58 (ent l50) was first detected after 48 h (400 au/ml), thereafter remaining stable up to 5 days. the effecti ... | 2006 | 17066915 |
| linking quantitative exposure assessment and risk management using the food safety objective concept: an example with listeria monocytogenes in different cross-contamination scenarios. | a practical approach was used to achieve specified food safety objectives (fsos) by incorporating risk assessment methodology. a series of cross-contamination scenarios at the retail level were evaluated in a spreadsheet and ranked according to their level of risk, and the degree to which they exceeded an fso for listeria monocytogenes (100 cfu/g) was determined. percentiles and analyses of variance were used to identify which scenarios would be most likely not to achieve the fso. it was also po ... | 2006 | 17066917 |
| evaluation of chemical and physical (high-pressure and temperature) treatments to improve the safety of minimally processed mung bean sprouts during refrigerated storage. | the objective of this study was to compare the effects of combined high hydrostatic pressure and temperature treatments with different chemical sanitation treatments (water, sodium hypochlorite, and hydrogen peroxide) on the microbiological properties of mung bean sprouts. in a first study, the raw product was subjected to several combined high-pressure and temperature treatments for calculating a mathematical model by a response surface methodology. the number of pressure-temperature (150 to 40 ... | 2006 | 17066918 |
| radiation sensitivity and postirradiation growth of foodborne pathogens on a ready-to-eat frankfurter on a roll product in the presence of modified atmosphere and antimicrobials. | intervention technologies including ionizing radiation, antimicrobials, and modified atmospheres (ma) can be used to inhibit the growth of or inactivate foodborne pathogens on complex ready-to-eat foods such as sandwiches. however, the effect of these technologies when used in combination (the hurdle concept) on the survival of foodborne pathogens is unknown. the ability of ionizing radiation to inactivate escherichia coli o157:h7, salmonella, listeria monocytogenes, and staphylococcus aureus in ... | 2006 | 17066924 |
| microbiological evaluation of sprouts marketed in mumbai, india, and its suburbs. | a study was undertaken to assess the microbiological quality of sprouts marketed in mumbai and its suburbs. a total of 124 sprout samples of four different legumes--mung (phaseolus aureus), matki (phaseolus aconitifolius), chana (cicer arietinum), and vatana (pisum sativum)--were analyzed over a period of 12 months for aerobic plate counts, coliforms, yeast and mold counts, staphylococci, salmonella, listeria monocytogenes, escherichia coli, e. coli o157:h7, and coagulase-positive staphylococcus ... | 2006 | 17066937 |
| isolation and pulsed-field gel electrophoresis typing of listeria monocytogenes from modified atmosphere packaged fresh-cut vegetables collected in ireland. | the incidence of listeria monocytogenes in modified atmosphere packaged fresh-cut fruits and vegetables from chill cabinets of a supermarket in ireland was investigated over a 2-year period. overall, 9.58% of fresh-cut produce was contaminated with listeria spp. various species of listeria were isolated from samples, including l. monocytogenes, l. seeligeri, l. innocua, l. welshimeri, and l. ivanovii. no fruit samples contained detectable l. monocytogenes. overall, a total of 21 l. monocytogenes ... | 2006 | 17066939 |
| effect of high-pressure treatment on the survival of listeria monocytogenes scott a in sliced vacuum-packaged iberian and serrano cured hams. | high-pressure treatment is useful for increasing the microbiological safety of ready-to-eat foods. with dry-cured hams, this treatment can be applied to the finished product after slicing and vacuum packaging. the effect of high-pressure treatment on the survival of inoculated listeria monocytogenes scott a and on the sensory characteristics of two spanish dry-cured hams, iberian and serrano, was investigated. ham slices were inoculated with l. monocytogenes at 6 x 10(6) cfu/g and held at 4 degr ... | 2006 | 17066942 |
| [prosthetic knee infection caused by listeria monocytogenes in a woman with rheumatoid arthritis and waldenström s macroglobulinemia]. | listeria are gram-positive bacilli that can be isolated from soil and in the normal fecal flora of many mammals. it is a uncommon pathogen in the general population, but immunocompromised individuals can develop several focal infections, most notably meningoencephalitis and sepsis. nevertheless, infectious arthritis caused by listeria monocytogenes is a exceptional event. we report a new case of prosthetic knee arthritis due to listeria in a woman with seropositive rheumatoid arthritis and walde ... | 2006 | 17067220 |
| fda approves use of bacteriophages to be added to meat and poultry products. | 2006 | 17067600 | |
| failure of listeria monocytogenes keratitis to respond to topical ofloxacin. | the purpose of this case report is to demonstrate the need for identification of the offending pathogen in bacterial keratitis to guide appropriate treatment. | 2006 | 17068464 |
| gliotoxin-mediated suppression of innate and adaptive immune functions directed against listeria monocytogenes. | gliotoxin is an immunosuppressive apoptogenic mycotoxin produced by a number of fungi including important human pathogens as aspergillus fumigatus. in order to elucidate the potential role of gliotoxin as immunoevasive fungal virulence factor we studied the effects of gliotoxin on the innate and adaptive t cell-mediated immune response against the facultatively intracellular bacterium listeria monocytogenes. gliotoxin induced apoptosis of bone marrow-derived macrophages, dendritic cells and cd8 ... | 2006 | 17071552 |
| cla-1 and its splicing variant cla-2 mediate bacterial adhesion and cytosolic bacterial invasion in mammalian cells. | cd36 and limpii analog 1, cla-1, and its splicing variant, cla-2 (sr-bi and sr-bii in rodents), are human high density lipoprotein receptors with an identical extracellular domain which binds a spectrum of ligands including bacterial cell wall components. in this study, cla-1- and cla-2-stably transfected hela and hek293 cells demonstrated several-fold increases in the uptake of various bacteria over mock-transfected cells. all bacteria tested, including both gram-negatives (escherichia coli k12 ... | 2006 | 17071747 |
| the mprf protein is required for lysinylation of phospholipids in listerial membranes and confers resistance to cationic antimicrobial peptides (camps) on listeria monocytogenes. | pathogenic bacteria have to cope with defence mechanisms mediated by adaptive and innate immunity of the host cells. cationic antimicrobial peptides (camps) represent one of the most effective components of the host innate immune response. here we establish the function of lmo1695, a member of the virr-dependent virulence regulon, recently identified in listeria monocytogenes. lmo1695 encodes a membrane protein of 98 kda with strong homology to the multiple peptide resistance factor (mprf) of st ... | 2006 | 17042784 |
| influence of particle size on drug delivery to rat alveolar macrophages following pulmonary administration of ciprofloxacin incorporated into liposomes. | in order to confirm the efficacy of ciprofloxacin (cpfx) incorporated into liposomes (cpfx-liposomes) for treatment of respiratory intracellular parasite infections, the influence of particle size on drug delivery to rat alveolar macrophages (ams) following pulmonary administration of cpfx-liposomes was investigated. cpfx-liposomes were prepared with hydrogenated soybean phosphatidylcholine (hspc), cholesterol (ch) and dicetylphosphate (dcp) in a lipid molar ratio of 7/2/1 by the hydration metho ... | 2006 | 17043040 |
| combined effect of mild heat and acetic acid treatment for inactivating escherichia coli o157:h7, listeria monocytogenes and salmonella typhimurium in an asparagus puree. | this study was conducted to validate combined heat and acid treatments for inactivating escherichia coli o157:h7, listeria monocytogenes and salmonella typhimurium in an acidified brine containing, or pickled, asparagus model food. | 2006 | 17040238 |
| occurrence and antibiotic sensitivity of listeria monocytogenes strains isolated from oysters, fish, and estuarine water. | we analyzed the presence of listeria spp. in oyster, fish, and seawater samples and tested isolates for antibiotic sensitivity. listeria monocytogenes was found in 4.5% of fish samples and 8.3% of seawater samples and was not recovered from oysters. multiresistant environmental strains were found, representing a potential threat to human health. | 2006 | 16980425 |
| species-specific differences in the activity of prfa, the key regulator of listerial virulence genes. | prfa, the master regulator of lipi-1, is indispensable for the pathogenesis of the human pathogen listeria monocytogenes and the animal pathogen listeria ivanovii. prfa is also present in the apathogenic species listeria seeligeri, and in this study, we elucidate the differences between prfa proteins from the pathogenic and apathogenic species of the genus listeria. prfa proteins of l. monocytogenes (prfa(lm) and prfa*(lm)), l. ivanovii (prfa(li)), and l. seeligeri (prfa(ls)) were purified, and ... | 2006 | 16980455 |
| listeria monocytogenes flagella are used for motility, not as adhesins, to increase host cell invasion. | flagellar structures contribute to the virulence of multiple gastrointestinal pathogens either as the effectors of motility, as adhesins, or as a secretion apparatus for virulence factors. listeria monocytogenes is a food-borne, gram-positive pathogen that uses flagella to increase the efficiency of epithelial cell invasion (a. bigot, h. pagniez, e. botton, c. frehel, i. dubail, c. jacquet, a. charbit, and c. raynaud, infect. immun. 73:5530-5539, 2005; l. dons, e. eriksson, y. jin, m. e. rottenb ... | 2006 | 16982842 |
| plasmid dna delivery by d-alanine-deficient listeria monocytogenes. | optimal dna vaccine efficacy requires circumventing several obstacles, including low immunogenicity, a need for adjuvant, and the costs of purifying injection grade plasmid dna. bacterial delivery of plasmid dna may provide an efficient and low-cost alternative to plasmid purification and injection. also, the bacterial vector may exhibit potential as an immune adjuvant in vivo. thus, we elected to examine the use of cell-wall-deficient listeria monocytogenes as a dna delivery vehicle in vitro. f ... | 2006 | 17022679 |
| modulation of cell adhesion and motility in the immune system by myo1f. | although class i myosins are known to play a wide range of roles, the physiological function of long-tailed class i myosins in vertebrates remains elusive. we demonstrated that one of these proteins, myo1f, is expressed predominantly in the mammalian immune system. cells from myo1f-deficient mice exhibited abnormally increased adhesion and reduced motility, resulting from augmented exocytosis of beta2 integrin-containing granules. also, the cortical actin that co-localizes with myo1f was reduced ... | 2006 | 17023661 |
| [comparison of the antibiotics sensibility pattern of listeria monocytogenes and salmonella spp. strains isolated from food with clinical origin samples]. | the actual use of antibiotics includes, not just its therapeutic cases, but also for disease prevention and as a growth promoter in animals. these practices have resulted in the propagation of resistance to antibiotics, representing a threat for public health. in this work, the antibiotic sensibility pattern of 20 listeria monocytogenes and 40 salmonella spp. strains, isolated from foodstuff was studied and compared with the antibiotic sensibility patterns of 20 l. monocytogenes and 100 salmonel ... | 2006 | 17024963 |
| cd8alpha+ dendritic cells are required for efficient entry of listeria monocytogenes into the spleen. | in addition to their bridging function between innate and adaptive immunity, dendritic cells (dcs) may also contribute to primary resistance against infection. here we analyzed the role of dcs during infection with listeria monocytogenes by performing systemic in vivo depletion of these cells. we showed that cd8alpha(+) dcs were crucial for l. monocytogenes spreading and proliferation in the spleen. efficient and rapid uptake of l. monocytogenes by cd8alpha(+) dcs required the small gtpase rac1 ... | 2006 | 17027298 |
| [listeria monocytogenes meningitis in an immunocompetent child]. | 2006 | 17029934 | |
| increased susceptibility to bacterial superinfection as a consequence of innate antiviral responses. | the reason why severe localized or systemic virus infections enhance and aggravate bacterial superinfection is poorly understood. here we show that virus-induced ifn type i caused apoptosis in bone marrow granulocytes, drastically reduced granulocyte infiltrates at the site of bacterial superinfection, caused up to 1,000-fold higher bacterial titers in solid organs, and increased disease susceptibility. the finding that the innate antiviral immune response reduces the antibacterial granulocyte d ... | 2006 | 17030789 |
| molecular evidence for vertical transmission of listeriosis, taiwan. | the case is presented of a pregnant woman at the 31st week of gestation with listeria monocytogenes bacteraemia and microabscess formation in the endometrium, who delivered an infant with disseminated infection (meningitis and bacteraemia). the two patients were successfully treated with intravenous ampicillin and gentamicin. molecular typing using random amplified polymorphic dna (rapd) analysis disclosed that three isolates from the mother (blood) and infant (blood and cerebrospinal fluid) had ... | 2006 | 17030924 |
| prevalence and lineages of listeria monocytogenes in chinese food products. | this study was undertaken to investigate the prevalence and lineages of listeria monocytogenes in different kinds of food products in local chinese markets. | 2006 | 17032231 |
| detection and characterization of listeria monocytogenes in sao jorge (portugal) cheese production. | listeria monocytogenes is a foodborne pathogen that can cause serious invasive disease in humans. because human listeriosis cases have previously been linked to consumption of contaminated cheese, control of this pathogen throughout the cheese production chain is of particular concern. to understand the potential for l. monocytogenes transmission via são jorge cheese, a portuguese artisanal cheese variety that bears a protected denomination of origin classification, 357 raw milk, curd, natural w ... | 2006 | 17033035 |
| isolation of pathogenic listeria monocytogenes in faeces of wild animals in captivity. | the isolation of pathogenic listeria spp. in faecal samples of captive wild animals was studied. isolation of the pathogen was attempted from the samples by selective enrichment in university of vermont medium and plating onto dominguez-rodriguez isolation agar, palcam agar and modified mcbride listeria agar. pathogenicity of the isolates was tested by christie, atkins, munch petersen test, phosphotidylinositol-specific phospholipase c assay, mice inoculation test and chick embryo bioassay. list ... | 2006 | 17034860 |
| dna fingerprinting by eric-pcr for comparing listeria spp. strains isolated from different sources in san luis, argentina. | in this study, a total of 24 listeria spp. strains were analyzed. twenty-two isolates were obtained in san luis (argentina) from human, animal, and food samples. two types of strains, listeria monocytogenes clip 22762 and listeria innocua clip 74915, were included as reference strains. all isolates were biochemically identified and characterized by serotyping, phage typing, and amplification of the flaa gene by polymerase chain reaction (pcr). repetitive intergenic consensus (eric) sequence-base ... | 2006 | 17037249 |
| the crystal structure of the bacteriophage psa endolysin reveals a unique fold responsible for specific recognition of listeria cell walls. | bacteriophage murein hydrolases exhibit high specificity towards the cell walls of their host bacteria. this specificity is mostly provided by a structurally well defined cell wall-binding domain that attaches the enzyme to its solid substrate. to gain deeper insight into this mechanism we have crystallized the complete 314 amino acid endolysin from the temperate listeria monocytogenes phage psa. the crystal structure of plypsa was determined by single wavelength anomalous dispersion methods and ... | 2006 | 17010991 |
| resistance of listeria monocytogenes biofilms to sanitizing agents in a simulated food processing environment. | the objective of this study was to evaluate the resistance of biofilms of listeria monocytogenes to sanitizing agents under laboratory conditions simulating a food processing environment. biofilms were initially formed on stainless steel and teflon coupons using a five-strain mixture of l. monocytogenes. the coupons were then subjected to repeated 24-h daily cycles. each cycle consisted of three sequential steps: (i) a brief (60 s) exposure of the coupons to a sanitizing agent (a mixture of pero ... | 2006 | 17012587 |
| construction of a multiple fluorescence labelling system for use in co-invasion studies of listeria monocytogenes. | existing virulence models are often difficult to apply for quantitative comparison of invasion potentials of listeria monocytogenes. well-to-well variation between cell-line based in vitro assays is practically unavoidable, and variation between individual animals is the cause of large deviations in the observed capacity for infection when animal models are used. one way to circumvent this problem is to carry out virulence studies as competition assays between 2 or more strains. this, however, r ... | 2006 | 17014739 |
| h2-m3-restricted t cells participate in the priming of antigen-specific cd4+ t cells. | h2-m3-restricted cd8+ t cells provide early protection against bacterial infections. in this study, we demonstrate that activated h2-m3-restricted t cells provide early signals for efficient cd4+ t cell priming. c57bl/6 mice immunized with dendritic cells coated with the mhc class ii-restricted listeriolysin o peptide llo(190-201) (llo) generated cd4+ t cells capable of responding to listeria monocytogenes (lm) infection. inclusion of a h2-m3-restricted formylated peptide fmigwii (fmig), but not ... | 2006 | 17015693 |
| detection of listeria monocytogenes from food samples by pcr after ims-plating. | for rapid, accurate and sensitive detection of listeria monocytogenes in food samples, colonies developed on the selective agar (oxford agar) after immunomagnetic separation (ims) were subjected to polymerase chain reaction (pcr) assay with the prf a1-2 primer pair. the proposed assay system was shown experimentally to be capable of specifically detecting the bacteria from food samples contaminated at more than 10(2) cfu/g. however, the enrichment culture after a short period of 16 h with the ap ... | 2006 | 17017131 |
| novel method to identify source-associated phylogenetic clustering shows that listeria monocytogenes includes niche-adapted clonal groups with distinct ecological preferences. | while phylogenetic and cluster analyses are often used to define clonal groups within bacterial species, the identification of clonal groups that are associated with specific ecological niches or host species remains a challenge. we used listeria monocytogenes, which causes invasive disease in humans and different animal species and which can be isolated from a number of environments including food, as a model organism to develop and implement a two-step statistical approach to the identificatio ... | 2006 | 17021104 |
| general model, based on two mixed weibull distributions of bacterial resistance, for describing various shapes of inactivation curves. | cells of listeria monocytogenes or salmonella enterica serovar typhimurium taken from six characteristic stages of growth were subjected to an acidic stress (ph 3.3). as expected, the bacterial resistance increased from the end of the exponential phase to the late stationary phase. moreover, the shapes of the survival curves gradually evolved as the physiological states of the cells changed. a new primary model, based on two mixed weibull distributions of cell resistance, is proposed to describe ... | 2006 | 17021197 |
| a homolog of bacillus subtilis trigger factor in listeria monocytogenes is involved in stress tolerance and bacterial virulence. | molecular chaperones play an essential role in the folding of nascent chain polypeptides, as well as in the refolding and degradation of misfolded or aggregated proteins. they also assist in protein translocation and participate in stress functions. we identified a gene, designated tig, encoding a protein homologous to trigger factor (tf), a cytosolic ribosome-associated chaperone, in the genome of listeria monocytogenes. we constructed a chromosomal delta tig deletion and evaluated the impact o ... | 2006 | 17021213 |
| lymphocyte apoptosis as an immune subversion strategy of microbial pathogens. | apoptosis is a component of cellular death in several immunological reactions. lymphocyte apoptosis is a feature of negative selection of thymic lymphocytes. target cells die by apoptosis during their interaction with cytotoxic t cells. antigens derived from apoptotic cells can be cross-presented by antigen presenting cells (apcs). in these examples, apoptotic death is a beneficial feature for the individual. the apoptosis of cells also occurs during infection with a variety of microorganisms, b ... | 2006 | 16997632 |
| in vitro antibacterial activity of bovine dialyzable leukocyte extract. | the rapidly developing resistance of many infectious pathogenic organisms to modern drugs has spurred scientists to search for new sources of antibacterial compounds. one potential candidate, bdle (dialysis at 10 to 12 kda cut-off) and its fractions ("s" and "l" by 3.5 kda cut-off and i, ii, iii, and iv by molecular exclusion chromatography), was evaluated for antibacterial activity against pathogenic bacterial strains (staphylococcus aureus, streptococcus pyogenes, lysteria monocytogenes, esche ... | 2006 | 16997795 |
| use of dna arrays for the analysis of outbreak-related strains of listeria monocytogenes. | we evaluated the discriminative power and usefulness of the dna array technology as compared to dna macrorestriction pattern analysis for monitoring epidemiologically related clusters of listeria monocytogenes strains that differ slightly in dna macrorestriction patterns. we show that this approach allows clarifying the genetic basis of the pattern variations. in the reported outbreak, the differences were due to phage excision, showing the power of this technique in epidemiological studies. | 2006 | 17002895 |
| antibacterial agent discovery using thymidylate synthase biolibrary screening. | thymidylate synthase (ts, thya) catalyzes the reductive methylation of 2'-deoxyuridine 5'-monophosphate to 2'-deoxythymidine 5'-monophosphate, an essential precursor for dna synthesis. a specific inhibition of this enzyme induces bacterial cell death. as a second round lead optimization design, new 1,2-naphthalein derivatives have been synthesized and tested against a ts-based biolibrary, including human thymidylate synthase (hts). docking studies have been performed to rationalize the experimen ... | 2006 | 17004711 |
| listeria monocytogenes subgroups iiia, iiib, and iiic delineate genetically distinct populations with varied pathogenic potential. | listeria monocytogenes lineage iii strains belonging to subgroups iiia (n = 8), iiib (n = 5), and iiic (n = 6) were examined along with other known serotype strains (n = 11) by pcr and southern hybridization using several recently described species-, virulence-, and serotype-specific primers and probes. the virulence of seven representative lineage iii strains was then evaluated in mice via the intraperitoneal route. the results suggest that subgroup iiia consists of typical rhamnose-positive av ... | 2006 | 17005751 |
| a truncated bacillus subtilis dal gene with a 3' ssra gene tag regulates the growth and virulence of racemase-deficient listeria monocytogenes. | listeria monocytogenes (lm) is a gram-positive intracellular pathogen that can elicit strong cellular immunity. an attenuated strain (lmdd) with deletions in two genes (dal and dat) required for d-alanine synthesis and viability has been shown to induce long-lived protective systemic and mucosal immune responses in mice when administered in the presence of the required amino acid. to bypass the necessity for exogenous d-alanine without compromising the safety of the original strain, the defect o ... | 2006 | 17005988 |
| first report of listeria monocytogenes endocarditis treated with linezolid. | 2006 | 17008067 |