Publications

t-lymphocyte clones responsive to shigella flexneri.	t lymphocytes from a patient with shigella flexneri dysentery and postdysenteric reactive arthritis were cloned by limiting dilution with recombinant interleukin-2 and a strain of s. flexneri different from that which had infected her. five of eight clones produced proliferated in response to the shigellae used to generate the clones. the response required irradiated syngeneic blood mononuclear cells as antigen-presenting cells. one such clone, mc12, proliferated in response to both the shigella ...	1989	2654177
studies with antibodies to cultured rat glomerular epithelial cells. subepithelial immune deposit formation after in vivo injection.	to investigate the role of glomerular epithelial cell (gec) membrane proteins in the in situ formation of subepithelial immune deposits, the authors raised a rabbit antiserum against gec that had been grown in culture (anti-gec). by indirect immunofluorescence (if) on normal rat kidney, anti-gec stained proximal tubular brush border (bb). after intravenous injection into animals, granular glomerular capillary wall staining for igg was present by ie and subepithelial immune deposits were identifi ...	1989	2655461
control of diarrheal diseases.	the tremendous advances made in the control of diarrheal diseases in the past few years indicate what more can be achieved. even though the lives of an estimated three quarters of a million children are being saved each year, over three million children are still dying from readily preventable diarrheal disease. the challenge is to build on the successes thus far, to learn from experience, to promote changes in health habits that will prevent diarrhea, and to make all of these activities sustain ...	1989	2655632
inoculum size in shigellosis and implications for expected mode of transmission.		1989	2656880
[determination of shigella antigens in the feces of people, immunized with enteral vaccine prepared from s. flexneri antigen].	the dynamic determination of the presence of the specific antigen and its activity in the excreta of humans subjected to enteral immunization with vaccine prepared from s. flexneri antigen was made in the agglutination test and neutralization test with the use of, respectively, antibody and antigenic erythrocyte diagnosticums. in the feces and urine of the vaccinees antibodies occurred less commonly and, as a rule, they were less active than those detected in dysentery patients at the correspond ...	1989	2658436
oral shigella vaccines.		1989	2659269
the enteric immune response to shigella antigens.	mucosal immunity to some enteropathogens occurs naturally following infection. by learning how to optimize initiation of the mucosal immune response it will be possible to develop vaccines against a wide variety of enteropathogens and their toxic products. in the past few years, we have examined stimulation of the mucosal response to shigella antigens. we have found that the mucosal memory response to shigella lps can be stimulated by oral immunization with live, but not with killed shigella. th ...	1989	2659270
electropherotyping of plasmid dna of different serotypes of shigella flexneri isolated in bangladesh.	one hundred and twenty-five shigella flexneri strains, isolated during january-december 1984, at the dhaka treatment centre of the international centre for diarrhoeal disease research, bangladesh, were serotyped using absorbed rabbit antisera specific for all type- and group-factor antigens, as well as a group of ten mouse and rat monoclonal antibodies. electropherotypes of the plasmid deoxyribonucleic acid (dna) were also determined. s. flexneri 2a was the predominant serotype followed by 3b, 1 ...	1989	2661251
renal allograft rejection: possible involvement of lymphokine-activated killer cells.	human renal allograft tissue was recovered at transplant nephrectomy from three patients with irreversible loss of graft function. this tissue was disaggregated and separated into two fractions on the basis of particle size. fraction 1 contained glomeruli and developed a mixed outgrowth containing adherent epithelial and mesangial cells after a limited period of culture. fraction 2 contained fragments of renal tubules and produced monolayers of tubular epithelial cells during culture. a populati ...	1989	2661417
inhibition of proteoglycan synthesis alters extracellular matrix deposition, proliferation, and cytoskeletal organization of rat aortic smooth muscle cells in culture.	arterial proteoglycans have been implicated in several important physiological processes ranging from lipid metabolism to regulation of smooth muscle cell growth. vascular smooth muscle (vsm) cells are the major producers of proteoglycans in the medial layer of blood vessels. to study functional consequences of alterations in vsm proteoglycan metabolism we used 4-methylumbelliferyl-beta-d-xyloside to inhibit proteoglycan synthesis in primary and early passage cultures of rat aortic smooth muscle ...	1989	2661564
[biological properties of bacteria of the species shigella flexneri].	some biological properties of s. flexneri have been studied. the strains of this bacterial species have been shown to produce dnaase in 98.8 +/- 0.77% of cases and rnaase in 97.4 +/- 1.5% of cases. the capacity for the positive reaction with congo red as early as after 24-hour incubation in the thermostat has proved to be characteristic of s. flexneri (91.1 +/- 3.6%). if stored at 4 degrees c in semiliquid agar, s. flexneri cultures have been found to retain their capacity for producing the abov ...	1989	2662686
lineage-specific gene rearrangement/deletion: a nonconservative model.	for the lymphocytic descendants of the hematopoietic differentiation pathway, characteristic gene rearrangements result in deletions of significant portions of chromosome regions specifying lymphocyte-specific gene products on either or both chromosomes of each involved pair. molecular mechanisms facilitating the rearrangement/deletion events have been elegantly and fruitfully elaborated in the years since the first documentation of their occurrence by hozumi and tonegawa (n. hozumi and s. toneg ...	1989	2663139
elimination of malignant clonogenic breast cancer cells from human bone marrow.	autologous bone marrow transplantation is a promising approach to the treatment of breast cancer but is at present limited to patients without bone marrow metastases. to eliminate malignant clonogenic breast cancer cells from normal human bone marrow, immunomagnetic separation has been combined with chemoseparation using 4-hydroperoxycyclophosphamide. breast cancer cell lines have been mixed with a 10-fold excess of irradiated human bone marrow from normal donors. mixtures have been incubated wi ...	1989	2663144
chloroplast and nuclear genomes of chlamydomonas reinhardtii share homology with escherichia coli genes for dna replication, repair and transcription.	most of the cpdna genes studied to date are genes encoding elements of the photosynthetic apparatus and translational machinery. much less is known about genes encoding the polypeptides involved in transcription, cpdna replication, recombination and repair. the similarities between bacterial and some cpdna genes were exploited to identify some of these chloroplast genes using bacterial probes. probes derived from the escherichia coli genes dnaa, reca, uvrc, transcriptional factor rho, and rpoc w ...	1989	2663186
congo red-mediated regulation of levels of shigella flexneri 2a membrane proteins.	the ability of shigella spp. to bind congo red from agar medium is generally correlated with their virulence properties. we used a metabolically active culture of shigella flexneri 2a to determine the effect of congo red on its membrane protein profiles. virulent s. flexneri grown in the presence of congo red at 37 degrees c showed increased levels of three proteins with mrs of 43,000, 58,000, and 63,000 (43k, 58k, and 63k proteins) in the sarkosyl-soluble membrane fractions. the observed phenom ...	1989	2663721
plasmid-associated adherence of shigella flexneri in a hela cell model.	the initial interaction of shigella flexneri with hela cells was studied at 4 degrees c, a temperature that inhibits parasite-directed endocytosis. it was found that invasive strains were 10-fold more adherent to hela cells than were isogenic, noninvasive strains which had lost a 140-megadalton plasmid. adherent strains were also more hydrophobic than were nonadherent strains.	1989	2663729
use of monoclonal antibodies to type shigella flexneri in bangladesh.	a panel of 10 mouse and rat monoclonal antibodies specific for different type- and group-specific o-antigenic determinants of shigella flexneri lipopolysaccharide was used to serotype 240 isolates of s. flexneri from bangladesh. three immunoglobulin m antibodies were used in a direct slide agglutination test; seven immunoglobulin g antibodies were absorbed to staphylococcus aureus and used in a coagglutination assay. all but 13 of the isolates could be serotyped by using the monclonal antibodies ...	1989	2666435
percutaneous nephrostomy in pyonephrosis.	a series of 76 pyonephrotic kidneys in 73 patients were drained by percutaneous nephrostomy (pn) tube and examined to evaluate the contribution of this technique to the treatment of pyonephrosis. in 71 patients, clinical symptoms disappeared 24-48 h after the procedure. two patients died from sepsis subsequent to anuria and underlying malignancy. once the acute phase had remitted, interventional procedures were carried out in 39 cases, and constituted the definitive therapy in 36. in 32 cases, e ...	1989	2667249
mechanics of the healed meniscus in a canine model.	the mechanical behavior of the intact canine stifle joint was studied, using a surgical model of meniscal injury and repair. thirty-eight animals were divided into five study groups: group s received only a sham arthrotomy (without a meniscal incision), groups p13 and p26 received peripheral medial meniscal incisions, and groups r13 and r26 received radial medial meniscus incisions. all meniscal incisions were repaired anatomically with absorbable suture. groups p13 and r13 were sacrificed at 13 ...	1989	2667375
internalization of shigella flexneri into hela cells occurs without an increase in cytosolic ca2+ concentration.	pathogenic shigella flexneri invades epithelial cells through directed phagocytosis. the role of intracellular free calcium ([ca2+]i) as a signal for this process was tested. no variation in the [ca2+]i could be detected by using the fluorescent indicator fura 2 to measure the [ca2+]i in hela cells during the invasion process. in addition, neither clamping nor maximal decreasing or increasing of the [ca2+]i of hela cells had any effect on their susceptibility to invasion. these data demonstrate ...	1989	2668195
purification, crystallization, and preliminary x-ray diffraction studies of the flavoenzyme mercuric ion reductase from bacillus sp. strain rc607.	the flavoenzyme mercuric ion reductase from bacillus sp. strain rc607 was purified by dye-ligand affinity chromatography. the protein was crystallized from solutions of high ionic strength, and one of the two crystal forms obtained has proven suitable for x-ray diffraction studies. preliminary analysis showed that these crystals belong to the tetragonal space group 1422. the unit cell dimensions are a = b = 180.7 a; c = 127.9 a. the diffraction pattern extends to better than 3 a resolution. crys ...	1989	2668287
molecular cloning and characterization of chromosomal virulence region kcpa of shigella flexneri.	in shigella flexneri, in addition to several well-recognized plasmid-borne virulence loci, at least three genetic loci implicated in pathogenesis have been recognized on the chromosome. to understand more about the pathogenesis of bacillary dysentery at a molecular level, the genetically recognized but previously unidentified kcpa region (one of the chromosomal regions near pure) was cloned and sequenced. a single translatable open reading frame encoding a 12310 dalton protein corresponding to t ...	1989	2668687
fungistatic activity of miconazole against candida albicans in relation to ph and concentration of nonprotonated drug.	at less than 10(-5) m, miconazole (mcz) exerts a fungistatic effect on candida albicans, presumably by interfering with ergosterol biosynthesis. the imidazole moiety of mcz is subject to protonation (pka approximately 6.5). based on pka and greater water solubility of protonated (mczh+) versus nonprotonated (mczo) drug, fungistatic action ought to be markedly affected by environmental ph, but apparently it is not. in this report growth phase, ph, and concentrations of mcz and mczo have been stud ...	1989	2668754
[bacillary dysentery--1987].		1989	2669032
liver disease associated with exposure to 1,1,1-trichloroethane.	1,1,1-trichloroethane is a halogenated hydrocarbon solvent commonly used in industry because of its supposed lack of hepatotoxicity. nonetheless, animal studies performed by several independent groups have shown the solvent to induce fat deposition, vacuolar degeneration, and centrilobular necrosis, changes similar to those seen after exposure to carbon tetrachloride, albeit of a much reduced magnitude, in animals exposed to the agent. four patients with fatty liver disease whose work entailed s ...	1989	2669664
oviducal storage of spermatozoa in the turkey: its relevance to artificial insemination technology.	1. this paper addresses questions concerning the role of oviducal sperm selection and storage in the relative inefficiency of turkey reproduction. 2. before the onset of egg production, the turkey has the biological potential to store spermatozoa in its oviduct for periods up to 5 weeks; after the onset of production fewer spermatozoa are retained by the oviduct. 3. a better understanding of the biological mechanisms which regulate sperm selection and storage in the turkey oviduct is necessary t ...	1989	2670069
putative start codon ttg for the regulatory protein virg of the hairy-root-inducing plasmid pria4.	the nucleotide sequence of the virg gene for a transcriptional activator on the agropine-type hairy-root-inducing plasmid pria4 was determined. the sequence contained one possible open reading frame. the gene product with a molecular size of 26.5 kda was identified by an escherichia coli coupled-transcription-translation system using cloned virg plasmids as templates. however, neither an atg nor a gtg start codon which could give rise to such a protein was identified in the nucleotide sequence. ...	1989	2670679
interleukin-1 potentiates granulopoiesis and thrombopoiesis by producing hematopoietic factors in vivo.	in vivo administration of recombinant human interleukin-1 beta (rhu il-1 beta) selectively enhanced the recovery from granulocytopenia and thrombocytopenia caused by whole body irradiation, in a dose dependent manner. since il-1 itself in vitro had no colony-stimulating activity (csa), we studied whether il-1 can produce hematopoietic factors in vivo, which in turn will promote granulopoiesis and thrombopoiesis. serum from il-1 injected mice showed marked granulocyte/macrophage csa (gm-csa), but ...	1989	2671565
synthesis of prostacyclin and thromboxane a2 by rat gastric mucosa. comparison of 1,6-dimethyl-4-oxo-1,6,7,8,9,9a-hexahydro-4h-pyrido (1,1-a)-pyrimidine-3-carboxamide and cimetidine.	the production of prostacyclin (pgi2) and thromboxane a2 (txa2) was studied in an indometacin-induced ulcer model in rats. the specific activities of prostacyclin synthetase and thromboxane synthetase as well as the tissue content of these prostaglandins were determined. the anti-ulcer effect of cimetidine and a novel agent, i.e. 1,6-dimethyl-4-oxo-1,6,7,8,9,9a-hexahydro-4h-pyrido(1,1-a)-pyrimidine-3- carboxamide (chinoin-127) was compared in this respect. the indometacin treatment shifted the b ...	1989	2673252
computer-assisted structural analysis of polysaccharides with an extended version of casper using 1h- and 13c-n.m.r. data.	the computer program casper, used in the structural analysis of polysaccharides composed of repeating units, has been extended. the extended version uses either unassigned 1h- or 13c-n.m.r. chemical shifts or the complete unassigned c,h-correlation spectrum, and can predict the structure of linear and branched oligo- and poly-saccharides. the number of possible structures, consistent with sugar and methylation analysis, can be decreased by the use of 1jc,h and 3jh,h values. the database, which c ...	1989	2673508
clinical & bacteriological profiles of shigellosis in calcutta before & after an epidemic (1984-87).	patients below 5 yr of age, hospitalised for shigellosis over a period of four years (1984-87), were studied. during the epidemic of bacillary dysentery (1984) isolation of different shigella spp. as well as shigella dysenteriae type 1 was high. decreased isolation of sh. dysenteriae type 1 and increased isolation of sh. flexneri was observed during post-epidemic years (1985-87). isolation of different shigella spp. was always above 25 per cent from patients with dysentery and greater than 7 per ...	1989	2674001
identification of shigella invasion genes by isolation of temperature-regulated inv::lacz operon fusions.	penetration and multiplication within cells of the human colonic epithelium are hallmarks of shigella spp. pathogenicity. shigella spp. virulence is regulated by growth temperature. strains phenotypically virulent when grown at 37 degrees c are phenotypically avirulent when grown at 30 degrees c. the number of genes involved in shigella spp. pathogenicity and how many virulence genes are temperature regulated are unknown. to facilitate the study of temperature-regulated virulence in shigella spp ...	1989	2674014
studies on interactions of endotoxin with factors of the contact system of plasma.		1989	2675058
lymphocyte homing.	the phenomenon of "homing" is discussed with respect to patterns of lymphocyte circulation and the molecules on the surface of both endothelium and lymphocytes that mediate this process. in addition, the data are analysed in the context of a model for lymphocyte homing.	1989	2675225
transcription by rna polymerase i stimulates mitotic recombination in saccharomyces cerevisiae.	the recombination-stimulating sequence hot1 is derived from the ribosomal dna array of saccharomyces cerevisiae and corresponds to sequences that promote transcription by rna polymerase i. when inserted at a chromosomal location outside the ribosomal dna array, hot1 stimulates mitotic recombination in the adjacent sequences. to investigate the relationship between transcription and recombination, transcription promoted by hot1 was directly examined. these studies demonstrated that transcription ...	1989	2677675
a 101-kilodalton heme-binding protein associated with congo red binding and virulence of shigella flexneri and enteroinvasive escherichia coli strains.	the ability of shigella flexneri to bind congo red or hemin is associated with virulence. a 101-kilodalton (kda) protein responsible for this phenotype (crb+) in s. flexneri was identified by a tetramethylbenzidine staining procedure which detects heme-protein complexes in polyacrylamide gels. labeling of cell-surface polypeptides with 125i revealed that the 101-kda heme-binding protein is expressed on the cell surface. expression of the protein was regulated by growth temperature and was found ...	1989	2680975
orientation of reaction center complexes from rhodobacter sphaeroides in proteoliposomes and the effect of o-phenanthroline on electrogenesis during primary photochemical reaction.	the orientation of rhodobacter sphaeroides reaction center complexes (rc complexes) in proteoliposomal membranes was investigated by a direct electrometric method. conditions were found that allow monitoring of only that rc complex fraction that is oriented with its donor side to the inner part of the proteoliposome. it is shown that o-phenanthroline, an inhibitor of electron transfer between primary (qa) and secondary (qb) quinone acceptors, can also inhibit the photoinduced qa reduction. the e ...	1989	2681179
herpes simplex infection associated with pemphigus vulgaris. case report and literature review.	a 55-year-old man had an 8-week history of oral ulcers. studies revealed concurrent pemphigus vulgaris and active herpes simplex infection. there have been seven previous case reports of this association, and these are reviewed.	1989	2681299
indicators of spontaneous and stimulated nitroblue tetrazole test of polymorphous-nuclear leucocytes in acute dysentery patients.	140 healthy individuals and 93 sick with acute dysentery were subjected to an examination by spontaneous and by bacterial preparations stimulated reaction with nitroblue tetrazole (nbt test). indicators in healthy persons were normal in the spontaneous, and increased in the nbt test, stimulated by bacterial preparations. indicators of the spontaneous nbt test in patients with acute dysentery were raised with a maximum in the period of early convalescence. stimulation by a live shigella culture-- ...	1989	2681402
the presence of nicotine on root surfaces of periodontally diseased teeth in smokers.	there is a growing body of scientific evidence to support the concept that the use of tobacco products significantly contributes to the progression of periodontal disease or is detrimental to healing following periodontal therapy. several studies have shown toxic effects of nicotine on peripheral circulation and the immune response. the purpose of the present study was to identify and compare the quantity of nicotine present on root planed and nonroot planed surfaces of teeth from smokers. twent ...	1989	2681674
mechanism of action of gnrh-induced oocyte maturation.		1989	2681744
mucoepidermoid carcinoma arising in a lymphoepithelial cyst--report of a case and review of the literature on branchiogenic carcinoma.	a 59-year-old japanese man who had recently noticed a gradual enlargement in a nodule located in the left anterior part of his neck was admitted for surgical treatment. the histopathological features of the resected tumor were consistent with a typical lymphoepithelial cyst, however, the inner surface epithelium of the cyst showed histological transition to mucoepidermoid carcinoma, which was partially invading the cyst wall. despite clinical efforts in a systemic search, no primary lesions whic ...	1989	2681906
[coexistence of abdominal aortic aneurysm and intraabdominal malignancy; two case reports].	we experienced two cases of abdominal aortic aneurysm which had intra-abdominal malignancy (early gastric cancer). case 1 was a 72 year-old man who was treated by two-stage operation of them. gastrectomy was performed about 7 months prior to the aneurysmectomy. case 2 was a 70 year-old man who was diagnosed both lethal diseases, renal dysfunction, chronic respiratory failure and multiple ventricular arrhythmia. he was treated by one-stage operation and was discharged with no complications. coexi ...	1989	2682201
a comparison of structural features of the walls of coronary arteries from 10 different species.	the intimal thickness of the coronary arteries of humans less than 20 years of age was compared with that 9 other species. it was found that humans were unique amongst the species studied in showing substantial intimal thickening at an early age. the intimal thickening was associated with defects of the internal elastic lamina (iel), which in humans were not repaired, but which in other species were associated with an effective reduplication of the iel. this reformed iel appeared in other specie ...	1989	2682490
thymotaxin, a chemotactic protein, is identical to beta 2-microglobulin.	thymotaxin, an 11-kilodalton protein chemotactic for rat bone marrow hematopoietic precursors, was purified from media conditioned by a rat thymic epithelial cell line. the nh2-terminal sequence of thymotaxin was identical to that of rat beta 2-microglobulin (beta 2m). antibodies to beta 2m removed thymotaxin activity from the fraction containing the 11-kilodalton protein. chemotactic activity was observed with rat plasma beta 2m, human beta 2m, and mouse recombinant beta 2m, further supporting ...	1989	2683083
construction and evaluation of a double mutant of shigella flexneri as a candidate for oral vaccination against shigellosis.	based on studies on the genetic and molecular basis of shigella flexneri invasive properties, we have constructed and evaluated a double mutant of s. flexneri serotype 5 for utilization as a live attenuated oral vaccine against shigellosis. the first mutation, icsa, blocks intracellular spread of bacteria as well as cell-to-cell infection. it affects the capacity of the invasive pathogen to form large abscesses in epithelia. the second mutation, iuc, eliminates production of the siderophore aero ...	1989	2683460
interspecies gene transfer in vivo producing an outbreak of multiply resistant shigellosis.	in 1983, a small outbreak of infections caused by a previously unrecognized multiply-drug-resistant shigella flexneri 3a strain occurred on the hopi indian reservation. the index patient, a diabetic woman with recurrent escherichia coli bacteriuria on prophylactic trimethoprim/sulfamethoxazole (tmp/smz) therapy, was hospitalized with concurrent e. coli urinary tract infection and shigellosis. both e. coli isolated from her urine and s. flexneri isolated from her stool were resistant to ampicilli ...	1989	2685126
[problems in the epidemiology and prevention of intestinal infections].		1989	2686152
[modern theoretical and practical conceptions of the epidemiology of shigellosis].		1989	2686310
multicentre experience in the treatment of burns with autologous and allogenic cultured epithelium, fresh or preserved in a frozen state.	this report describes the clinical results obtained from a multicentre experience of the use of autologous and allogenic cultured human epidermal cells in the treatment of partial and full skin thickness burns. a laboratory has been organized to supply cultured epithelium to burns units in different cities. from may 1986 to december 1988, 58 patients with an age range of 1 to 59 years, and with burns covering between 7 and 95 per cent of the body surface area, have been treated. graftable cultur ...	1989	2686683
a novel maturation marker for human sertoli cells.	the maturation of sertoli cells at puberty is critical for the initiation and maintenance of spermatogenesis. little is known about the factors which control sertoli cell maturation. we have examined sertoli cells in testicular specimens from 44 subjects, ranging in age from 6 months to 67 years, for reactivity with a mouse monoclonal antibody (m2a) using the immunoperoxidase reaction. we found positive reactivity with prepubertal but not postpubertal sertoli cells, suggesting that the antigen d ...	1989	2687179
use of shigella flexneri ipac and ipah gene sequences for the general identification of shigella spp. and enteroinvasive escherichia coli.	the products of the ipab, ipac, and ipad genes are involved in the expression of the invasive phenotype in all species of shigella and enteroinvasive escherichia coli (eiec). dna probes derived from these genes are accurate indicators of the invasive phenotype (m. venkatesan, j. m. buysse, e. v. vandendries, and d. j. kopecko, j. clin. microbiol. 26:261-266, 1988); however, spontaneous loss of the invasion plasmid or selective deletion of invasion-associated genes may restrict the usefulness of ...	1989	2687318
isoenzyme analysis of typhoid-shigella and escherichia-shigella hybrid vaccines and their parental strains.	isoenzyme analysis can be used to measure phenotypic changes and to characterize bacterial strains. the use of isoenzyme analysis to characterize bacterial hybrid vaccine strains that were prepared for human vaccination is described in this report. the hybrid vaccine strains were two lots of salmonella typhi-shigella sonnei vaccine, two lots of escherichia coli-shigella flexneri vaccine, and their parental strains. the two lots of s. typhi-s. sonnei hybrid vaccine had only 5 of 11 reactive isoen ...	1989	2687324
complement component c1q enhances invasion of human mononuclear phagocytes and fibroblasts by trypanosoma cruzi trypomastigotes.	internalization and infectivity of trypanosoma cruzi trypomastigotes by macrophages is enhanced by prior treatment of parasites with normal human serum. heating serum or removing c1q from serum abrogates the enhancement, but augmentation of attachment and infectivity is restored by addition of purified c1q to either serum source. although both noninfective epimastigotes (epi) and vertebrate-stage tissue culture trypomastigotes (tct) bind c1q in saturable fashion at 4 degrees c, internalization b ...	1989	2687332
the induction of interferon production in fibroblasts by invasive bacteria: a comparison of salmonella and shigella species.	as the role of interferon (ifn) in host defense against facultative intracellular bacterial infections continues to expand, it has become increasingly important to understand what cell types can produce ifn following infection and/or interaction with these invasive bacteria. we have demonstrated previously that shigella flexneri was able to induce high levels of ifn in primary cultures of human and murine fibroblasts following bacterial invasion. in this study, we examined the ability of salmone ...	1989	2687612
monoclonal antibodies against the surface antigens of shigella flexneri serotype 1b and shigella dysenteriae serotype 1.	monoclonal antibodies against the surface antigens of shigella flexneri 1b and s. dysenteriae 1 were prepared. the specificities of the antibodies were evaluated by enzyme-linked immunosorbent assay (elisa), and quantitative agglutination using microtiter plate. monoclonal antibodies against s. flexneri 1b, designated sf2b2 and sf2g4, belonged to igg2a and igg1 subclass, respectively. the former was specific for s. flexneri 1b, whereas the latter was reactive not only to s. flexneri 1b, but also ...	1989	2687648
toxic megacolon and perforation caused by shigella.		1989	2688808
shigella colitis with radiological and endoscopic correlation: case report.	we report a patient with acute colitis secondary to shigella flexneri infection. radiologic and endoscopic studies were obtained during and after the patient's clinical recovery. hypotonic air contrast barium enemas were helpful in evaluating the severity and extent of the disease proximal to the segment viewed by flexible sigmoidoscopy. they showed pseudopolyposis, which has not been previously reported in the acute phase, and other mucosal abnormalities which persisted long after clinical reco ...	1989	2688842
nutritional anestrus in beef cows: concentrations of glucose and nonesterified fatty acids in plasma and insulin in serum.	twenty-two nonlactating hereford cows exhibiting normal estrous cycles were fed either maintenance (m) or restricted (r) diets until most of the r cows became anestrus; r cows then were fed 160% of the m diet until estrous cycles resumed. concentrations of progesterone, glucose, insulin and nonesterified fatty acids (nefa) were determined in weekly blood samples. blood also was collected frequently, before and after i.v. infusion of 300 ml of a 40% glucose solution, to evaluate responses in bloo ...	1989	2689418
biofilm model experiments in vitro with escherichia coli and shigella strains.	escherichia coli and shigella were cultured together in a glucose-free minimal medium completed only by hog gastric mucin. in mixed cultures inoculated simultaneously one member of the pair more or less overgrew the other. this organism remained predominant when superinfected with its strain pair after 1, 3, 5 or 7 days incubation. other signs of the quasi biofilm character of such cultures are the higher level of free polysaccharides and enhanced viscosity in the medium and higher streptomycin ...	1989	2690554
[field trials with an oral cell-free vaccine made from shigella flexneri].	the data on the study of the reactogenicity, safety and prophylactic potency of a new acellular vaccine prepared from s. flexneri 2a antigenic complexes are presented. according to the results of two epidemic experiments, the vaccine, introduced by oral administration, showed low reactogenicity, safety and sufficient prophylactic potency. the vaccine decreased morbidity rate in dysentery caused by s. flexneri 2a and ensured the protection of 74% (72-80%) of the vaccinees. the complete course of ...	1989	2692352
platelet-activating factor increases platelet-dependent glycoconjugate secretion from tracheal submucosal gland.	using isolated glands from feline trachea, we examined the effect of platelet-activating factor (paf) on radiolabeled glycoconjugate release and glandular contraction by measuring induced tension in the absence or presence of platelets. paf alone did not produce any significant glandular contraction nor any significant change in glycoconjugate release from isolated glands. in the presence of purified platelets containing no plasma, paf (10(-8) to 10(-5) m) produced significant glycoconjugate sec ...	1989	2692465
falciparum malaria--present day problems. an experience with 425 cases.	clinical details and present day problems encountered in 425 cases of falciparum malaria (pf) are reported. 10.11% had taken chloroquine prior to reporting to us. parasitic count done in 23.05% cases lacked correlation with severity of disease. pattern of fever varied markedly but 5.4% were afebrile throughout and presented only with bodyache and malaise. apyrexial spell was noted in 5.64%. 28.70% had typical facial looks of anaemia and sallow complexion. cerebral symptoms were noted in 3.05%. o ...	1989	2693436
the genetics of protein targeting in escherichia coli k12.	genetic analysis of protein targeting in escherichia coli has been facilitated by the use of lacz gene fusions. strains which contain a fusion between a gene specifying a noncytoplasmic protein and lacz exhibit novel phenotypes that can be exploited to obtain export-defective mutations. analysis of these mutations has demonstrated the importance of the signal sequence and provided some insight into the functions performed by this complex intragenic export signal. in addition, genes that specify ...	1989	2693457
[clinical trial of t-3262 (tosufloxacin tosilate) on salmonella enteritis, and fecal drug concentration and change in the fecal microflora in the acute diarrheal patients. japan research committee of t-3262, research group for acute infectious enteritis].	for the purpose of evaluation of clinical efficacy, safety and usefulness on salmonella enteritis, t-3262 (tosufloxacin tosilate), a newly developed pyridone-carboxylic acid derivative, was administered to a total of 103 patients and carriers. in addition, in vitro antibacterial activity of t-3262 was determined against the clinical isolates, and compared with those of nalidixic acid (na), pipemidic acid (ppa), enoxacin (enx), norfloxacin (nflx) and ofloxacin (oflx). and when t-3262 was administ ...	1989	2693543
[the role of the antilysozyme activity of shigella flexneri as a factor controlling the degree of its resistance to the lytic action of bacteriophages].	the level of the antilysozyme activity of s. flexneri in ensuring the high level of their phage resistance has been studied. the realization of the phage protective effect of antilysozyme activity has been noted to occur due to disturbances in the lysis of infected bacteria by phage-synthetized lysozyme-like enzyme. the direct relationship between the level of the lysozyme production of bacteriophages and their capacity for overcoming the antilysozyme protection of the host bacterium has been sh ...	1989	2694689
[interleukin-1-inducing activity of the polysaccharide-containing antigens of the cell wall in yersinia pestis].	the induction of the synthesis of interleukin-1 (il-1) in human monocytes under the influence of the endotoxic preparations (lps) and y. pestis basis somatic antigen has been experimentally studied. the results obtained in this study make it possible to come to the conclusion that the capacity of the endotoxin of y. pestis cell wall, consisting of lps of type r, for inducing the synthesis of il-1 in human monocytes is not different from the corresponding capacity of salmonella and shigella lps, ...	1989	2694696
[resistance of shigella to antibiotics].	antibiotic sensitivity of 104 shigella clinical strains and 104 escherichia coli strains isolated from patients with acute dysentery not treated with antibiotics in 1986-1987 was studied. it was shown that 100 per cent of the dysentery pathogens and colon bacilli were antibiotic resistant. strains resistant simultaneously to chloramphenicol, ampicillin, streptomycin, tetracycline, monomycin and kanamycin were the most frequent among the dysentery pathogens. colon bacilli and dysentery pathogens ...	1989	2694990
extra-intestinal manifestations of shigellosis.		1989	2695518
[molecular pathogenesis of bacillary dysentery caused by shigella flexneri].		1989	2695659
evidence for clathrin mobilization during directed phagocytosis of shigella flexneri by hep2 cells.	the enteroinvasive species shigella flexneri expresses a plasmid-mediated capacity to penetrate epithelial cells by directed phagocytosis involving actin polymerization. in the present work, hep2 cells were depleted of intracellular k+ in order to arrest receptor-mediated endocytosis and to evaluate the role of this endocytic pathway in the internalization of invasive microorganisms. such a treatment, which efficiently inhibited diphtheria toxin endocytosis and dissociated clathrin coats of the ...	1989	2695740
genotoxicity testing: phage t7 inactivation test of various furan and arenofuran derivatives.	the genotoxic activities of 28 furan and arenofuran derivatives were tested by the phage t7-inactivation test. the genotoxic activity of the compounds was characterized quantitatively. all the compounds studied have pronounced genotoxic activities in our system. empirical rules relating structure to genotoxic activity were found. data obtained with our system were compared with the results of other biological systems (salmonella assay, sos chromotest, cho/hgprt, gene amplification) in the case o ...	1989	2695762
characterization and autoprocessing of precursor and mature forms of human immunodeficiency virus type 1 (hiv 1) protease purified from escherichia coli.	a recombinant plasmid encompassing the human immunodeficiency virus type 1 (hiv 1) protease coding sequence and flanking regions (ala-13 to gly-185 of the pol open reading frame) has been expressed in two distinct strains of escherichia coli, ar58 and ar68. in the first strain, ar58, the primary translation product, a 25 kilodalton (kda) precursor protein, is short-lived and rapidly processes itself to the 11 kda mature protease in vivo. in the second strain, ar68, the 25 kda species is only par ...	1989	2695927
fulminating encephalopathy associated with shigella flexneri.		1989	2696551
[clinico-morphological criteria of diagnosis of dysentery and salmonella infections].	clinical symptoms and laboratory findings have been evaluated for 108 patients with intestinal infection associated with apparent colitic syndrome. differential diagnosis between dysentery and salmonellosis was not an easy task even though clinical signs of the diseases appeared different. dysentery ran with tenesmus, sigmoid pain and spasms. symptoms of gastritis, intoxication, dehydration, hepatomegaly predominated in salmonellosis. in obscure cases colonic biopsy can be helpful due to high se ...	1989	2696830
transferable trimethoprim resistance of shigellae encountered in vellore (south india).	shigella flexneri and sh. shigae which are the two most common shigellae encountered in vellore (south india) were found to exhibit resistance to trimethoprim and trimethoprim-sulphamethoxazole. eighty four per cent of this was high level resistance. transfer studies conducted with these strains indicated that this high level resistance is plasmid mediated.	1989	2697688
protein-losing enteropathy.		1989	2697925
[the etiological structure of shigellosis in the ussr in recent years].	the characterization of etiological structure of shigella infection in the whole of the ussr, in individual union republics and at a number of other administrative territories of the ussr in recent years is presented. s. flexneri has been shown to prevail at the territories with unsatisfactory water supply of the population, and s. sonnei prevails at the territories with good water supply. at the former territories s. dysenteriae and s. boydii retain their etiological importance, while at the la ...	1989	2698030
[the role of delayed hypersensitivity in the development of an experimental dysentery infection and vaccinal process].	the results of experiments, indicating the development of cell-mediated delayed hypersensitivity (dh) skin reaction, its dependence on the virulence of shigella flexneri 2a, the dose and the character of the antigen used for testing the reaction, are presented. one of the immunologically active components of s. flexneri virulent strain 2a no. 516 is a biologically active thermolabile factor detected in filtrate and supernatant fluid. these data suggest that the immunosuppressive action of the vi ...	1989	2698032
clinical role of granulocyte-macrophage colony-stimulating factor.	the activity of recombinant human granulocyte-macrophage colony-stimulating factor (gm-csf) for increasing leukocyte production and enhancing mature neutrophil function has been clearly demonstrated in phase i clinical trials in patients with a variety of hematologic and malignant diseases. the focus of current studies includes determination of optimal administration schedules and its use in combination with myelosuppressive chemotherapy, radiation therapy or antimicrobial agents. the utility of ...	1989	2698875
[prostaglandins and cyclic nucleotides in the gastrointestinal form of yersiniosis].	in spite of the resemblance of the clinical picture of gastrointestinal yersiniosis and acute dysentery, material differences underlie the pathogenesis of these diseases. yersiniosis is marked by the predominance of an increase in the content of pgf2 alpha, whereas acute dysentery by an increase in the content of pge, which may be accounted for by greater intensity of the allergic manifestations in yersiniosis patients as compared with dysentery. shigellosis runs its course in the presence of th ...	1989	2699095
[the importance of immunofluorescence and cytologic indices in the diagnosis and prognosis of the course of dysentery].		1989	2699099
effects of endothelin on the cardiovascular system and on smooth muscle preparations in different species.	(1) the actions of porcine endothelin (et), an endothelium-derived vasoconstrictor peptide, have been investigated in several in vitro smooth and cardiac muscle preparation as well as on the blood pressure of anaesthetized rats and rabbits. (2) in isolated visceral smooth muscles (guinea pig ileum, rat colon and uterus, rabbit jejunum) et caused a long-lasting contraction which persisted after repeated rinsing. et did not interfere with the spontaneous phasic activity of the rabbit jejunum or wi ...	1989	2699356
production and characterization of monoclonal antibodies with diagnostic potential against shigella flexneri.	highly specific monoclonal antibodies (mcabs), 1am3, 2am1 and 2bm2 were produced and characterized against sh. flexneri 1a, 2a and 2b respectively. iam3 is an igg3 (lambda) type antibody reactive against whole bacteria or the lipopolysaccharide (lps) only; 2am1 is an igg1 (kappa) type antibody which reacts with the polysaccharide component of lps; 2bm2 is an igg3 (lambda) type antibody which binds with both the polysaccharide and protein/peptide components of the lps. the results indicate that s ...	1989	2700207
current management of salivary gland tumors. part 2.	the authors base their treatment of salivary gland malignancies on the size of the primary and the histopathologic diagnosis. group 1 includes smaller tumors in the t1 and t2 classification with cell types that are associated with slow growth. a parotidectomy is usually sufficient therapy for tumors in this group. group 2 contains t1 and 2 tumors with more aggressive behavior. total parotidectomy is indicated here, with postoperative radiotherapy. t3 tumors and patients with nodal metastasis or ...	1989	2701414
[resonance stimulation of regional ventilation of the lungs by sound vibration in the prevention of acute respiratory insufficiency].		1989	2704716
abnormalities in the levels of extracellular and tissue amino acids in the brain of the seizure-susceptible rat.	basal and high potassium-stimulated release of endogenous amino acids was measured using brain dialysis in the hippocampus of urethane-anesthetized seizure-resistant (sr) and seizure-susceptible (ss) rats. moreover, the tissue level of amino acids was determined in the hippocampus, sensorimotor cortex, cerebellum and corpus striatum. the basal extracellular concentration of amino acids did not differ between sr and ss rats. however, aspartate release was higher, and taurine and phosphoethanolami ...	1989	2707250
preliminary notes on vulnerability of the retina to injury from peripheral laser exposure conditions.	if laser exposures to the periphery of the retina from low-energy lasers cause a hemorrhage obscuring the optical pathway to the fovea, resulting in loss of visual function, the risks for soldiers on the battlefield may be greater than previously expected. two off-axis experiments were undertaken to obtain an indication of the hazards involved in exposing the peripheral retina to visible laser irradiation. both off-axis exposures caused immediate vitreal hemorrhages in the periphery. after 80 mi ...	1989	2708055
differential expression of transforming growth factors alpha and beta in rat intestinal epithelial cells.	expression of transforming growth factor alpha (tgf alpha), and transforming growth factor beta (tgf beta) was assessed in isolated primary rat intestinal epithelial cells as well as a rat intestinal crypt cell-derived cell line (iec-6). a gradient in tgf beta was present, with high concentrations of a 2.5-kb transcript found in undifferentiated crypt cells and progressively lower amounts of the tgf beta transcript in increasingly differentiated villus cell populations. in contrast, the concentr ...	1989	2708531
topographic organization of the central projections of the spiral ganglion in cats.	the morphological organization of inputs from restricted sectors of the cat cochlear spiral ganglion into the cochlear nucleus was studied by making focal extracellular injections of horseradish peroxidase (hrp) into the spiral ganglion. injections resulted in golgi-like labeling of a small cluster of spiral ganglion cells and their peripheral and central axons. large injections involved most of the cells within rosenthal's canal in sectors of the spiral ganglion innervating greater than or equa ...	1989	2708585
tyrosine phosphorylation in the postnatal rat brain: a developmental, immunohistochemical study.	we have used antibodies against phosphotyrosine to probe 50-microns cerebellar sections from rats of various ages as well as sections of adult brainstem, cerebrum, and olfactory bulb to investigate the developmental appearance of this phosphorylation system as revealed by light microscopy. while the overall intensity of staining in the cerebellum was highest at 7 days, the pattern of staining in the adult is quite disparate from that seen in younger animals. from 10 to 21 days postnatal, stainin ...	1989	2708590
effect of surfactant-associated protein-a (sp-a) on the activity of lipid extract surfactant.	the properties of natural bovine surfactant and its lipid extract have been examined with a pulsating bubble surfactometer which assesses the ability of surfactant lipids to adsorb to the air/liquid interface and reduce the surface tension to near 0 dynes/cm during dynamic compression. studies conducted at 1 mg/ml phospholipid revealed that the surface activity (i.e., the ability to produce low surface tensions) of lipid extracts could be enhanced by incubating the sample at 37 degrees c for 120 ...	1989	2713385
effect of estradiol on the phosphorylation of rat liver ribosome proteins.	the effect of estradiol on phosphorylation with gamma-32p-atp of ribosome proteins from polyribosome fraction, isolated from female rat liver is found out. rats (180 g) used were: sham-operated, ovariectomized and treated after ovariectomy for 11 days with 20 micrograms 17 beta-estradiol-dipropionate per 100 g body weight. the estradiol deficiency after ovariectomy decreased phosphorylation, but the estrogenization of ovariectomized rats increases it twice. in vitro the hormone (1-2 mumol/l) [co ...	1989	2714224
early plaque colonization on human cementum.	we have described the morphology of developing plaque on cementum in an in vivo human model. slabs of cementum obtained from sound teeth, rendered anorganic with 5% sodium hypochlorite, were glued to orthodontic brackets and positioned on the upper canines, premolars and molars in 8 volunteers. the brackets were removed after 2, 4, 8, 24 h and processed for scanning electron microscopy (sem). within 2 h, a thin pellicle covered the cementum surface, with few micro-organisms detectable. at 4 and ...	1989	2715365
production of monoclonal antibodies toward bovine interferons-alpha suitable for immunopurification.	five murine hybridoma clones, producing monoclonal antibodies (mabs) to bovine interferon-alpha (boifn-alpha) were established. one of these, f12, secreted mabs giving high titers, in elisa tests, neutralizing both boifn-alpha, -alpha c, and boifn-alpha d activities, belonging to the mouse igg1 class, and having a binding affinity constant of 10(8) m-1. f12 mabs were used for immunoaffinity purification of boifn-alpha, and recombinant boifn-alpha c from escherichia coli extracts was purified to ...	1989	2715671
immunoblotting analysis of the reaction of wildebeest, sheep and cattle sera with the structural antigens of alcelaphine herpesvirus-1 (malignant catarrhal fever virus).	malignant catarrhal fever (mcf) is a disease of cattle and some other ruminants caused by alcelaphine herpesvirus-1 (ahv-1), a virus of wildebeest. the disease also occurs in the absence of wildebeest and is then thought to be caused by a viral agent harboured by the sheep. the structural proteins of ahv-1 have been used as antigens for the immunoblotting analysis of sera from wildebeest, sheep and cattle infected by either ahv-1 or the "sheep-associated" form of the disease. wildebeest sera sho ...	1989	2718352
[viral infection and obstructive cholangiography in children].	in the last years it has been suggested that idiopathic obstructive cholangiopathy is a pathologic process with evolutive studies: extrahepatic biliary atresia and neonatal hepatitis. the possibility of a viral agent as the inciting factor has been investigated. this report shows four male and one female with confirmed idiopathic obstructive cholangiopathy with different clinical picture. there was evidence of cytomegalovirus infection in two patients, rubella infection in one patient, hepatitis ...	1989	2719422
a light microscopic and autoradiographic study of non-irradiated and irradiated ocular wounds.	focal gamma irradiation was used to limit the intraocular extension of scar tissue which typically occurs after posterior perforating injury to the eye. standard posterior perforating injuries were created in the right eye of forty-eight rabbits, half of which had the site of perforation focally irradiated using a cobalt 60 ophthalmic plaque. non-irradiated wounds healed with profuse formation of highly cellular and vascularised granulation tissue which invaded the vitreous to form contractile v ...	1989	2721224
a light microscopic and autoradiographic study of non-irradiated and irradiated ocular wounds.	focal gamma irradiation was used to limit the intraocular extension of scar tissue which typically occurs after posterior perforating injury to the eye. standard posterior perforating injuries were created in the right eye of forty-eight rabbits, half of which had the site of perforation focally irradiated using a cobalt 60 ophthalmic plaque. non-irradiated wounds healed with profuse formation of highly cellular and vascularised granulation tissue which invaded the vitreous to form contractile v ...	1989	2721224
the effects of ultrasonically induced hyperthermia on experimental tumors in the rabbit eye.	greene's melanomas, implanted in the anterior chamber of 31 eyes of 19 dutch rabbits, were treated with hyperthermia induced by high intensity focused ultrasound (hifu). the percentage necrosis 24 hr after treatment (n) as a function of steady-state intratumor temperature (t) could be described with n(t) = -181.5 + 4.7t (p less than 0.05). the most frequent side effect was a local hemorrhagic keratitis. the acoustic absorption coefficient and thermal conductivity of the tumor tissue were 0.08 cm ...	1989	2722440