Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| persistence, reactivation, and cell transformation by human herpeviruses: herpes simplex 1, 2 (hsv-1, hsv-2), cytomegalovirus (cmv), varicella-zoster (vzv), epstein-barr virus (ebv). | 1979 | 222414 | |
| analysis of chromosomes, nucleic acids, and polypeptides in hamster cells transformed by herpes simplex virus type 2. | syrian hamster embryo fibroblasts were oncogenically transformed by uv-inactivated herpes simplex type 2. eighteen clones were isolated shortly after transformation occurred. two clones and their tumor derivatives were studied using several techniques. the karyotype analysis revealed different chromosome patterns in the two clones and a tendency toward hypodiploidy in the tumor derivatives. all of these cell lines were shown by molecular hybridization to contain 40% of the hsv-genome in several ... | 1979 | 222446 |
| experimental encephalitis in the newborn rat due to herpes simplex virus type 2. | 1979 | 222606 | |
| thin-layer immunoassay for determination of antibodies to herpes simplex virus. | thin-layer immunoassay (tia) is a simple serological technique suitable for analysis of large numbers of samples. in this study, tia was evaluated for determination of antibodies to herpes simplex virus. herpes simplex virus antigen used in tia was purified from material released from virus-infected cells. the results obtained by tia were compared with those obtained by neutralization and complement fixation tests. tia was found to be as sensitive as the neutralization test for demonstration of ... | 1979 | 222798 |
| herpes virus infection as a cofactor in carcinogenesis: supernatants of herpes virus type 1- and type 2-infected cell cultures contain a cell growth-stimulating factor. | some cell cultures synthesize a mitogenic factor (dnasf) following their lytic infection with either hsv-1 or hsv-2 which is shed into the medium. of six cell lines permissive to hsv infection tested, three produce dnasf (cv1, hf, mca) and a significantly higher (3h)-tdr incorporation was obtained with indicator cells growing in supernatants of virus-infected cells (supv+) than in the corresponding supernatants of sham-treated cells (supv-). sometimes the values obtained with supv+ exceed those ... | 1979 | 223098 |
| some aspects of the role of viruses in cancer. | the cells of tumours induced by many oncogenic dna viruses, or cells transformed in vitro, contain virus-specific t and transplantation antigens; these have been described for sv40 virus, polyoma virus and adenoviruses. the investigation of viruses as causes of malignant disease in man has sought to establish whether tumour cells possess these virus-specific proteins; however, to date and with the limitations of present techniques, this enquiry has not demonstrated the above viruses as causal of ... | 1979 | 223141 |
| stimulation of human cellular thymidine kinase activity during abortive infection with herpes simplex virus type 2. | 1979 | 223308 | |
| association of herpes simplex virus (hsv) with cervical cancer by lymphocyte reactivity with hsv-1 and hsv-2 antigens. | twenty-six women with invasive cervical cancer were examined for lymphocyte responsiveness to phytohemagglutinin (pha) herpes simplex virus (hsv). blood specimens were obtained from each patient before radiation therapy and separated into subpopulations by ficoll-hypaque centrifugation. lymphocytes were cultured in rpmi-1640 containing autologous plasma and exposed to pha, hsv-1, hsv-2, and control antigens. the results compared with those of women having negative papanicolaou smears and matched ... | 1979 | 223438 |
| sites of integration of herpes simplex virus type-2 thymidine kinase gene in human chromosomes. | 1979 | 223994 | |
| production of monoclonal antibodies against nucleocapsid proteins of herpes simplex virus types 1 and 2. | we prepared mouse hybrid cell lines which produced antibodies against herpes simplex virus type 1 and 2 nucleocapsids. cell lines 1d4 and 3e1, respectively, secreted immunoglobulin g1 herpes simplex virus type 1 and immunoglobulin g1 herpes simplex virus type 2 antibodies which immunoprecipitated proteins designated p40 and p45 from homologous nucleocapsid preparations but precipitated no proteins from heterologous preparations. in contrast, guinea pig antisera prepared against either herpes sim ... | 1979 | 91687 |
| detection of herpesvirus type-specific antibody by a micro solid phase radioimmunometric assay. | a micro solid phase radioimmunometric assay has been developed which measures type-specific antibody in human sera to herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2). the test is performed by coating wells of microtiter plates with purified hsv-specified glycoproteins, vp123 and vp119, derived from hsv-1- and hsv-2-infected cells, respectively. the coated wells are then incubated with diluted unadsorbed sera and subsequently with radioiodinated goat anti-human igg reagent. because these m ... | 1979 | 94044 |
| herpes simplex virus type 2 and carcinoma of urinary bladder. | 1979 | 94309 | |
| iontophoretic application of adenine arabinoside monophosphate for the treatment of herpes simplex virus type 2 skin infections in hairless mice. | 1979 | 94335 | |
| microfluorometric quantitation of size, deoxyribonucleic acid and viral antigenic determinants in cells productively infected with hsv-2. | the interaction of herpes simplex virus type 2 (hsv-2) with the eukaryotic human cell line (hep-2) was investigated by flow microfluorometric analysis. the three parameters that were quantitated include cell size, deoxyribonucleic acid content and the expression of virus-specific antigens. productively infected cells are always smaller than uninfected ones, and they resolve into two populations with respect to viral antigenic content. consistent with viral replication, one of these two populatio ... | 1979 | 94525 |
| detection of herpes simplex virus infection of female genitalia by the peroxidase-antiperoxidase method alone or in conjunction with the papanicolaou stain. | the sensitive peroxidase-antiperoxidase (pxapx) method individually and in conjunction with the papanicolaou (pap) stain was used to detect herpes simplex virus (hsv) in specimens from human female genitalia. initial studies using a model system of hsv-1 or hsv-2-infected vero cells established (i) acetone as the most effective fixative, (ii) optimal dilutions of preimmunization and anti-hsv serum for differentiation of infected from noninfected cells, (iii) optimal concentration of 3,3'-diamino ... | 1979 | 94600 |
| immunoelectrophoretic identification and purification of herpes simplex virus antigens released from infected cells in tissue culture. | proteins released from herpes simplex virus type 1 (hsv-1)-and type 2 (hsv-2)-infected hep-2 cells have been characterized by the crossed immunoelectrophoretic technique. both hsv type-common and type-specific antigens were found in the tissue culture medium 24 h after infection. antigen ag-6, an hsv-1-specific antigen, was found in high concentration in the medium as compared to other hsv antigens released from hsv-1-infected cells. the hsv-2-specific antigens, ag-4 and ag-1, were released in m ... | 1979 | 85610 |
| a rapid technique for distinguishing herpes-simplex virus type 1 from type 2 by restriction-enzyme technology. | a rapid simplified technique, based on restriction-endonuclease analysis of radioactively labelled d.n.a. is described; it distinguishes herpes-simplex virus type 1 strains from herpes-simplex virus type 2 unambiguously. large numbers of isolates can be screened in 4--5 days. | 1979 | 86095 |
| herpes simplex virus (hsv) infection of organotypic nerve cell cultures. tubule-like structures in the nuclei of cells infected with type 2 but not with type 1 virus. | in hsv-2 infected cultures, intranuclear tubule-like structures were found more commonly in fibroblasts and central nervous system (cns) neurons than in oligodendrocytes, astrocytes, satellite and schwann cells, but were found least often in peripheral neurons. | 1979 | 525261 |
| observations of antigenic relatedness between viruses of the herpes simplex "neutroseron". | the antigenic relatedness of three viruses of the herpes simplex type 1 neutroseron - herpes simplex virus types 1 (hsv-1) and 2 (hsv-2) and bovine mammillitis virus (bmv) - has been examined by immune precipitation and virus neutralization tests. many virus-specific infected-cell polypeptides were shown to possess antigenic sites shared by both hsv-1 and hsv-2. cross-neutralization between the viruses is mediated through antibodies to at least two antigenic sites, one shared by hsv-1, hsv-2 and ... | 1978 | 87364 |
| antibody-dependent cell-mediated cytotoxicity to herpes simplex virus type 2 infected target cells in the course of cervical carcinoma. | the antibody-dependent cell-mediated cytotoxicity (adcc) assay was used as a sensitive instrument to study if specific antibodies acting synergistically with lymphocytes to induce target cell destruction, could be detected in sera from patients with cervical carcinoma. significant cytotoxicity to herpes simplex type 2 (hsv-2) infected target cells was observed in sera of 17 out of 23 (74%) surviving patients with cervical cancer who were followed with at least two serum samples. a rise in cytoly ... | 1978 | 101081 |
| increased susceptibility to infection with herpes simplex virus types 1 and 2 of cold-adapted l cells. | l cells (l-as subline) have been adapted to a temperature of 4 degrees c. in the cold-adapted cells, designated lc3, greater amounts of infectious herpes simplex virus types 1 (hsv-1) and 2 (hsv-2) were synthesized than in the original l-as cells or in another control l-cell line. two strains of hsv-1 reached higher infectious titres in lc3 cells grown at 36 degrees c than in those grown at 32 degrees c. the hsv-2 strain tested replicated in lc-3 cells grown at 32 degrees c better than at higher ... | 1978 | 26196 |
| biological effects of 5-carboxy-2'-deoxyuridine: hydrolysis product of 5-trifluoromethyl-2'-deoxyuridine. | 5-carboxy-2'-deoxyuridine (5-cooh-2'-durd) is a product of the base-catalyzed hydrolysis of 5-trifluoromethyl-2'-deoxyuridine. hydrolysis of 5-trifluoromethyl-2'-deoxyuridine to 5-cooh-2'-durd in phosphate-buffered saline was kinetically first order and was ph dependent. at 37 degrees c and ph 7.0, 7.5, and 8.0, hydrolysis occurred with rate constants of 4.19 x 10(-5), 9.30 x 10(-5), and 1.61 x 10(-4) s(-1), respectively, with corresponding half-lives of 45.7, 20.6, and 11.9 h. 5-cooh-2'-durd in ... | 1978 | 28691 |
| identification of a common antigen of herpes simplex virus bovine herpes mammillitis virus, and b virus. | in immunoelectrophoretic analyses one common antigen was demonstrated in antigen preparations from herpes simplex virus types 1- and 2- (hsv-1 and hsv-2), bovine herpes mammillitis (bhm) virus-, and b virus-infected cells solubilized by triton x-100. the antigen was also demonstrated in solubilized purified hsv-1 and bhm virus. the common antigen was identified as antigen 11 of hsv-1 or hsv-2. differences were found in the polypeptide composition of the related antigens when isolated from the fo ... | 1978 | 78988 |
| antibodies to herpes simplex virus type 2 in husbands of patients with cervical carcinoma. | 1978 | 79964 | |
| type-common cp-1 antigen of herpes simplex virus is associated with a 59,000-molecular-weight envelope glycoprotein. | the cp-1 antigen of herpes simplex virus type 1 (hsv-1) is a glycoprotein found in the soluble portion of infected cells, in detergent extracts of infected cell membranes, and in the envelope of purified virus. antisera were prepared against a further purified form of cp-1 prepared from hsv soluble antigen mix; a glycoprotein, gp52, isolated from detergent-treated infected cells; and detergent extracts of purified virus. each of the antisera reacted with cp-1 to give a single immunoprecipitin ba ... | 1978 | 80458 |
| [changes in the cellular surface, related to herpes simplex virus type 2, in cells derived from human cervical cancer]. | 1978 | 224426 | |
| physical mapping of herpes simplex virus-coded functions and polypeptides by marker rescue and analysis of hsv-1/hsv-2 intertypic recombinants. | a number of temperature-sensitive (ts) mutants and one pyrimidine deoxyribonucleoside kinase-deficient mutant of herpes simplex virus (hsv), have been located on the physical map of the genome by means of marker rescue experiments and by the analysis of the crossover points in intertypic recombinants between hsv types 1 and 2. the physical map is compared to the genetic map and certain anomalies identified. analysis of infected-cell polypeptides specified by intertypic recombinants has allowed t ... | 1978 | 221322 |
| analysis of herpes simplex virus low molecular-weight native proteins by polyacrylamide gel electrofocusing. | this communication has demonstrated the potential application of gel electrofocusing for the isolation and further characterization of hsv-2 native proteins. at least eight major hsv-2 specific proteins with characteristic isoelectric points could be reproducibly resolved by gel isoelectric focusing. their isoelectric points ranged from 5.0 to 7.5 and analysis by two-dimensional electrophoresis on sds gels indicated that their molecular weights range from 11,000 to 49,000. | 1978 | 221328 |
| the use of intertypic recombinants for analysis of gene organization in herpes simplex virus. | analysis of the dna sequence arrangement and polypeptides specified by 28 hsv-1 x hsv-2 recombinants show the following: (i) recombinants with heterogeneous l and s components or with heterogenous inverted repeats are viable. (ii) hsv-1 and hsv-2 genes appear to be functionally equivalent and with few exceptions co-linearly arranged. co-linear dna maps have been established. (iii) at most two arrangements of hsv dna are capable of replication. this is consistant with current studies suggesting t ... | 1978 | 221347 |
| repair of single-stranded breaks in host dna when rabbit kidney cells are infected with herpes simplex virus type 2. | 1978 | 221348 | |
| mapping of the herpes simplex virus dna sequences in three herpes simplex virus thymidine kinase-transformed cell lines. | we have made use of a novel filter hybridization approach in order to map the herpes simplex virus (hsv) dna sequences which are present in three hsv thymidine kinase (tk)-transformed cell lines. the cell line 33a+ which was produced by infection of 3t3 tk- cells with uv-irradiated hsv-2 (333) was found to contain one contiguous stretch of viral dna sequences which maps between 0.15 and 0.57 on the hsv-2 genome. the sequences mapping from 0.31 to 0.37 were present in 3--4-fold higher abundance t ... | 1978 | 221351 |
| dna-mediated transfer of herpes simplex virus tk gene to human tk- cells: properties of the transformed lines. | human tk- cells carrying the hsv-2 tk gene express a tk activity of viral origin and maintain the tk+ phenotype when grown in hat medium. under non-selective or counterselective conditions, however, reversion to a tk- phenotype occurs with a significant frequency characteristic of each transformed line. the tk- phenotype appears to be stable since no instances of tk- to tk+ reversion have been observed. | 1978 | 221353 |
| enhanced efficiency of transfection of hsv dna in hsv-1 dna-transformed hamster cells. | the efficiency of transfection, i.e., the number of plaques per microgram of dna, was compared for herpes simplex virus (hsv) type 1 and type 2, and herpesvirus eidolon (antigenically unrelated to hsv) dnas in hsv dna-transformed cells (eh/a44) and control hamster cells (eht). the efficiency of transfection of hsv-1 and hsv-2 dnas was significantly higher in eh/a44 cells than in eht cells, showing that an early step in hsv infection was involved in the partial resistance of hsv dna-transformed c ... | 1978 | 221357 |
| interferon in the replication of herpes simplex virus in normal and pathological nerve cells. | previous results obtained in experimental and clinical trials have demonstrated that topical combined thrapy with human interferon (hi) and human colostral secretory immunoglobulin a (s-iga) is effective against herpetic corneal infection. this therapy prevented encephalitis in rabbits but could not completely prevent recurrences either in rabbits or in patients. a number of in vitro studies were designed to elucidate the role of these factors in herpes simplex virus replication in the nervous s ... | 1978 | 221373 |
| immune responses to vaginal or systemic infection of mice with herpes simplex virus type 2. | relationships among cell-mediated immune (cmi) responses, neutralizing antibody, and macrophages in host resistance to vaginal or systemic infection with hsv-2 were defined in balb/c mice. the importance of t-lymphocytes in resistance to either systemic or vaginal infection was demonstrated by increased susceptibility of mice depleted of t-lymphocytes. a role for non-specific resistance against the systemic, but not vaginal, infection was demonstrated by increased susceptibility of mice depleted ... | 1978 | 221400 |
| detection of herpes simplex virus type 2 mrna in human cervical biopsies by in situ cytological hybridization. | 1978 | 221414 | |
| reactivation of herpes simplex virus (type 2) infection in trigeminal ganglia and oral lips with cyclophosphamide treatment. | age-dependent resistance in trigeminal nerve against type 2 herpes simplex virus infection through oral lips and reactivation of the latent virus infection with cyclophosphamide in the ganglion in correlation to theprimary infected part were studied in mice with immunofluorescent and histopathological methods. the mice acquired resistance in the nervous tissue after 8 weeks of age. the viral antigen appeared in all trigeminal ganglia immediately after infection, but not found at 4 weeks later. r ... | 1978 | 219274 |
| reduction of herpes simplex virus-type 2 plaque formation by urea. | the effect of urea on plaque formation by herpes simplex virus type 2 (hsv-2) was examined in two systems at concentrations within or approximating the range found in human urine. approximately 7--10 mg urea/ml, added 2, 4, or 8 hours after infection, reduced plaque formation by 50% in african green monkey kidney cells. the growth of this system was affected slightly by continuous treatment with urea at 7--10 mg/ml. plaque formation was reduced in the monkey kidney system, albeit diminishingly, ... | 1978 | 215721 |
| unusual membranous structures found in cells infected with herpes simplex virus type 2 maintained in media containing 2-deoxy-d-glucose. | 1978 | 215884 | |
| reversible inhibition of the induction of dna polymerase of herpes simplex virus type 2 in hela cells. | the induction of dna polymerase of herpes simplex virus type 2 in hela cells was inhibited by 2-mercapto-1-(beta-4-pyridethyl)benzimidazole added at an early time after infection. however, the inhibitory effect was easily released by removal of the inhibitor. since the drug disorganizes nucleolar structure and prevents its function in hela cells in a reversible manner, it is possible that nucleoli are involved in the induction of dna polymerase of this virus. | 1978 | 216336 |
| antibodies to herpes simplex virus types 1 and 2 in patients with squamous-cell carcinoma of uterine cervix in india. | antibody activity to herpes simplex virus type-1 (hsv-1) and type-2 (hsv-2) was measured by the indirect hemagglutination (iha) test in sera from 124 women with squamous-cell carcinoma of the uterine cervix, 46 women with non-cervical cancer and 116 matched normal women. the type-specific antibodies were assayed by iha inhibition test. the proportion of cervical cancer cases positive for hsv-2 infection was significantly greater than that of non-cervical cancer cases and matched control women. n ... | 1978 | 214407 |
| recombinants between herpes simplex virus types 1 and 2: analyses of genome structures and expression of immediate early polypeptides. | recombinants between temperature-sensitive mutants of herpes simplex virus types 1 (hsv-1) and 2 (hsv-2) were constructed. using restriction endonucleases, we analyzed the genome composition of 17 intertypic recombinants and detected crossovers in every region of the genome. the virion dna of one recombinant appeared to be largely "frozen" in two of the four possible genome arrangements of hsv. knowledge of the genome structures of recombinants enabled us to physically map immediate early polype ... | 1978 | 214575 |
| colposcopic examination of the cervix of cebus monkeys. | a total of 410 colposcopic examinations were performed on 188 female cebus monkeys that were under study to determine the oncogenic potential of herpes simplex virus type 2 in this genus. a split-cone vaginal speculum was developed that permitted good observation of the vaginal cervix in the cebus monkey. the cervical anatomy of cebus monkeys was found to differ from that of humans in that the surface of the animal cervix was more papilliform, with thinner squamous epithelium, and the squamocolu ... | 1978 | 214730 |
| effect of treatment with 5-ethyl-2'-deoxyuridine on herpes simplex virus encephalitis in normal and immunosuppressed mice. | 5-ethyl-2'-deoxyuridine (5-ethyl-durd), an analog of thymidine, was evaluated for its capacity to inhibit herpes simplex virus (hsv) replication in vitro and in vivo. the 50% effective dose concentration of 5-ethyl-durd for hsv types 1 and 2 (hsv-1 and -2) cultured in vero cells was 6 and 9 mug/ml, respectively. levels of 5-ethyl-durd 14-fold in excess of the 50% effective dose for hsv-1 did not inhibit the formation of confluent monolayers by vero cells, suggesting that the compound was not cyt ... | 1978 | 215081 |
| relationship of herpes simplex virus type-2 antibodies and squamous dysplasia to cervical carcinoma in situ. | serum specimens from 75 women with cervical carcinoma in situ, 84 with squamous dysplasia, and 132 controls, who had previously been interviewed and tested for complement fixing antibodies against a number of organisms, were analyzed for hsv-2 antibodies. carcinoma in situ and severe dysplasia were associated with hsv-2 antibodies. mild dysplasia was related to evidence of prior infection by trichomonas vaginalis, adenoviruses, and mycoplasma pneumoniae, plus a history of vaginal discharge. seve ... | 1978 | 215294 |
| comparison of various macrophage-inhibitory agents on vaginal and systemic herpes simplex virus type 2 infections. | pretreatment of mice intraperitoneally with silica, trypan blue, or dextran sulfate to inhibit macrophage function markedly increased the lethality of a systemic intravenous infection with herpes simplex virus type 2, but did not affect the lethality or local virus growth after vaginal infection of mice with herpes simplex virus type 2. agents which inhibit macrophage function by different mechanisms decreased host resistance to herpes simplex virus type 2, but the effects of macrophage-inhibito ... | 1978 | 215548 |
| production of group- and type-specific antigens during non-permissive infection of dog kidney cells with herpes simplex virus type 2. | 1978 | 203280 | |
| macrophages and age-dependent resistance to hepatitis induced by herpes simplex virus type 2 im mice. | an age-dependent increase in the resistance of balb/c mice to induction of focal necrotic hepatitis by herpes simplex virus type 2 was demonstrated. in 3-week-old mice inoculated intraperitoneally with virus, numerous necrotic foci developed in the liver. as the mice matured, the number of lesions declined until the age of 8 weeks, when no further increase in resistance appeared. corresponding to this, the virus titers of livers and spleens of 3-week-old mice were higher than in 8-week-old anima ... | 1978 | 203536 |
| radiometric detection of herpes simplex viruses. | two radiometric techniques were investigated as possible means of detecting viruses in clinical specimens. the effect of herpes simplex virus on dna synthesis by monolayers of human embryonic lung fibroblasts was monitored by the incorporation of [3h] thymidine or [125i] iododeoxyuridine ([125i] idu). radiometric results were compared with those obtained from visual examination for cytopathic effects in the same cell line. cells infected with herpes simplex virus type-2 (hsv-2, 10(6.8) virions) ... | 1978 | 203666 |
| herpes simplex virus type 2 induced pyrimidine nucleoside kinase: enzymatic basis for the selective antiherpetic effect of 5-halogenated analogues of deoxycytidine. | 1978 | 204334 | |
| herpes simplex virus type 2 and carcinoma of cervix uteri. | 1978 | 204721 | |
| chromosome abberations in rat embryo cells transformed after infection with ultraviolet-irradiated herpes simplex virus. | two rat embryo cell lines (tr1 and tr2) transformed after infection with ultaviolet-inactivated herpes simplex virus type 2 showed modal chromosome numbers in the tetraploid range. in the tr1 cell line a near-diploid cell population replaced during subcultivation the hypotetraploid cells thus explaining the loss of immunofluorescent viral antigens. both transformed cell lines showed frequent achromatic gaps. in the tr1 cell line such gaps were preferentially located in specific chromosome region ... | 1978 | 205041 |
| induction capacity and influence of dthdmp on thymidine kinase activity of type 1 and 2 strains of herpes simplex virus. | the thymidine kinase inducing ability of 104 strains of herpes simplex virus was studied comparatively. a pronounced relationship was established between induction of the enzyme and the serotype of the strains. as a rule, the strains of serotype 2 are weaker inducer of dthd- and dcyd-kinase activity than serotype 1 strains. a certain parallelism exists between induction of both enzymes, however the activity of the thymidine kinase increases after infection with herpes simplex virus 4--5 times mo ... | 1978 | 205197 |
| production of plasminogen activator by cells transformed by herpesviruses. | plasminogen activator is produced by hamster cells transformed by human herpesviruses. these cell lines have previously been shown to be oncogenic when injected s.c. into newborn syngeneic hamsters. lysis of fibrin overlays by these cell lines was plasminogen dependent. normal hamster embryo fibroblasts and a hamster cell line transformed by para-7 (an adenovirus-sv 40 hybrid) failed to produced lysis. in separate experiments fibrin overlay of lytically infected secondary rabbit kidney cells did ... | 1978 | 205345 |
| role of activated macrophages in resistance of congenitally athymic nude mice to hepatitis induced by herpes simplex virus type 2. | congenitally athymic nude (nu/nu) mice of a balb/c genetic background were found considerably more resistant to the induction of focal necrotic hepatitis by herpes simplex virus type 2 (hsv-2) tha, were phenotypically normal littermates (nu/+) or balb/c mice. the augmented resistance was age dependent, as it was only manifested in mice from 4 to 5 weeks of age. studies of the course of infection showed that nude mice were able to restrain virus multiplication in the liver far better than normal ... | 1978 | 205507 |
| the deoxyribonuclease induced after infection of kb cells by herpes simplex virus type 1 or type 2. i. purification and characterization of the enzyme. | the deoxyribonuclease induced in kb cells by herpes simplex virus (hsv) type 1 and type 2 has been purified. both enzymes are able to completely degrade single- and double-stranded dna yielding 5'-monophosphonucleotides as the sole products. a divalent cation, either mg2+ or mn2+, is an absolute requirement for catalysis and a reducing agent is necessary for enzyme stability. the maximum rate of reaction is achieved with 5 mm mgcl2 for both hsv-1 and hsv-2 dnase. the optimum concentration for mn ... | 1978 | 206546 |
| biochemical transformation of mouse cells by herpes simplex virus type 2: enhancement by means of low-level photodynamic treatment. | the biochemical transformation of thymidine kinase-deficient cells by uv-inactivated herpes simplex virus is enhanced by low-level photodynamic treatment of the infected cells. at the concentration of proflavine used, the virus was not inactivated and both virus and cellular dna syntheses were only marginally inhibited. the observed enhancement of the transfer of a virus gene to the cell genome suggests a possible cocarcinogenic role for photodynamically active dyes at very low concentrations. | 1978 | 206727 |
| resistance to vaginal or systemic infection with herpes simplex virus type 2. | 1978 | 207239 | |
| alkaline dnase activity in cells infected with a temperature-sensitive mutant of herpes simplex virus type 2. | bhk cells infected with the temperature-sensitive mutant ts13 of herpes simplex virus type 2 at a nonpermissive temperature lack the alkaline nuclease activity, which is induced by the mutant at a permissive temperature and by wild-type virus at either temperature. for ts13, enzyme activity could be induced by a temperature shift to permissive conditions, but not in the presence of cycloheximide. after a shift from permissive to nonpermissive conditions in the presence of cycloheximide, the acti ... | 1978 | 207883 |
| anatomy of herpes simplex virus (hsv) dna. x. mapping of viral genes by analysis of polypeptides and functions specified by hsv-1 x hsv-2 recombinants. | in an earlier paper (morse et al., j. virol 24:231--248, 1977) we reported on the provenance of the dna sequences in 26 herpes simplex virus type 1 (hsv-1) x hsv-2 recombinants as determined from analyses of their dnas with at least five restriction endonucleases. this report deals with the polypeptides specified by the recombinants and by their hsv-1 and hsv-2 parents. we have identified (i) the corresponding hsv-1 and hsv-2 polypeptides with molecular weights ranging from 20,000 to more than 2 ... | 1978 | 207894 |
| 5,6-dihydro-5-azathymidine: in vitro antiviral properties against human herpesviruses. | 5,6-dihydro-5-azathymidine (dhadt), a novel water-soluble nucleoside antibiotic, inhibits herpes simplex virus type 1 (hsv-1) in appropriately infected cell cultures to a greater extent than herpes simplex virus type 2 (hsv-2). vaccinia virus was less susceptible than hsv-2, and pseudorabies virus yields were not reduced at the concentrations studied. plaque formation by varicella-zoster virus was suppressed by dhadt. dhadt was slightly toxic to cells at concentrations that were inhibitory for h ... | 1978 | 208459 |
| protective effect of an oral infection with herpes simplex virus type 1 against subsequent genital infection with herpes simplex virus type 2. | the problem of whether oral herpes simplex virus type 1 (hsv-1) infection provides protection against subsequent genital infection by herpes simplex virus type 2 (hsv-2) was investigated. mice were used as models. following conditions in man, both the oral and genital infections applied were noninjurious. mice infected orally with hsv-1 were weakly protected against virus 'take' following vaginal challenge with hsv-2. genital 'takes' were found in 67% of the immunized mice, as compared with 83% ... | 1978 | 209295 |
| [herpes simplex virus type 2 and cervix cancer]. | 1978 | 209356 | |
| conditions required for induction of murine p30 by herpes simplex virus. | mouse cells (line n cia cl10) contain 1.2-2.5 ng murine leukaemia virus (mulv) p30 antigen/mg of protein; this amount of antigen is measurable by competition radioimmunoassay (ria) but is not detectable by indirect immunofluorescence (if). infection of n cia cl10 cells with herpes simplex virus type 2 (hsv-2) induces expression of mulv p30. induction by hsv-2 does not require either cell or virus dna synthesis and is optimal 8 h post infection when cells at 50-70% confluence are infected at a mu ... | 1978 | 210128 |
| a nonlytic transforming mutant of herpes simplex virus type 2. | a small-plaque mutant (no.69) of herpes simplex virus type 2 (hsv-2) strain 333 has been previously isolated and characterized in this laboratory. this mutant was shown to produce a high ratio of noninfectious to infectious particles when grown at the nonpermissive temperature in hamster embryo fibroblasts [westmoreland d. and rapp f. (1976). journal of virology [8:92--102]. in this study, we have demonstrated that it is possible to obtain noninfectious stocks of this virus which retain transfor ... | 1978 | 210252 |
| effect of immunization on the development of latent ganglionic infection in mice challenged intravaginally with herpes simplex virus types 1 and 2. | immunization of balb/c mice with virulent and avirulent strains of hsv-1 resulted in high levels of neutralizing antibody and protected against both the lethal effect of the virus and the development of a latent ganglionic infection when animals were challenged by the intravaginal route. in animals immunized with avirulent strain of hsv-2 and challenged with a high virulent strain of hsv-2, substantial protection against death was observed despite low levels of neutralizing antibody. nineteen pe ... | 1978 | 210669 |
| herpes simplex virus type 2 infection of neonatal mice. | we have demonstrated that newborn mice can be infected orally with herpes simplex virus type 2. transplacental and transvaginal transmission of herpes simplex virus type 2 did not occur in our mice. failure to produce natural transvaginal infection in mice may be explained by the relatively short period of exposure to the virus during parturition. these experiments demonstrate that high oral doses of herpes simplex virus type 2 can produce disseminate herpes neonatal infection in mice and theref ... | 1978 | 211091 |
| the fine structure of cells infected with temperature-sensitive mutants of herpes simplex virus type 2. | the fine structure of cells infected with the hg 52 strain of herpes simplex virus type 2 and 13 temperature-sensitive mutants derived from it was investigated. in cells infected with the wild-type virus, development of virions appeared to be similar to that described in previous reports. however there were two exceptions to this: (1) capsid envelopment apparently occurred de novo in the nucleus; (2) densely staining vacuolar accumulations were seen, frequently surrounding virus capsids. the 13 ... | 1978 | 211178 |
| complement-mediated cytolysis of hsv-1 and hsv-2 infected cells: plasma membrane antigens reactive with type-specific and cross-reactive antibody. | surface antigens of bhk-21 cells infected with hsv-1 or hsv-2 were radioiodinated (125i) with lactoperoxidase, immune precipitated and analysed by polyacrylamide gel electrophoresis (page). experiments using antiserum to hsv-1 or hsv-2, absorbed with appropriate hemotypic or heterotypic antigens, revealed that both type-specific (homotypic) and cross-reactive antibody combined with surface glycoproteins to form a single large radioactive peak. this peak, which constituted the major glycoprotein ... | 1978 | 211189 |
| herpes simplex virus necleic acid synthesis following infection of non-permissive xc cells. | dna hybridization kinetic analysis of cellular dna following high multiplicity infection of non-permissive xc cells by herpes simplex virus type i showed that hsv dna penetrates to the nucleus of the cell but that the number of virus dna copies present in each cell quickly begins to decline. there did not appear to be any net virus dna synthesis and the loss of virus dna copies continued until there was approximately one per haploid genome equivalent. hsv-2 likewise did not show any detectable v ... | 1978 | 211197 |
| herpes simplex virus types 1 and 2 in organotypic cultures of mouse central and peripheral nervous system. i. light microscopic observations of myelin degeneration. | mature mouse spinal cord-ganglion cultures, which contain both peripheral and central nervous system as one unit, were infected with herpes simplex virus type 1 (hsv 1) or type 2 (hsv 2) and observed by bright field microscopy for up to 72 hours. there was degeneration of both central and peripheral myelin in cultures infected with either virus, but the pattern of peripheral myelin degeneration associated with hsv 1-infected cultures was differrnt from that in hsv 2-infected cultures. type 1 was ... | 1978 | 211204 |
| evidence for a herpes simplex virus-specific factor controlling the transcription of deoxypyrimidine kinase. | a cistron-specific, enzyme-forming-capacity method was used to study the control of herpes simplex virus (hsv)-specific deoxypyrimidine kinase (dpyk) mrna synthesis. a virus-specific factor was formed by a primary infecting virus, and this factor effected the transcription of dpyk mrna of a superinfecting virus in the presence of cycloheximide, suggesting that the factor acted in "trans" and was a diffusible one. after the addition of actinomycin d to prevent further transcription and upon remov ... | 1978 | 211259 |
| cellular immune response in genital herpes simplex virus infection. | we studied the relations between the cellular immune response, pre-existing complement-fixing antibody and virus type with duration of virus excretion in genital herpes simplex virus (hsv) infection. thirty-six patients (seven with hsv-1 and 29 with hsv-2) with genital herpes underwent serologic testing, sequential viral cultures and weekly determination of lymphocyte-transformation stimulation index with inactivated hsv antic n. the duration of virus excretion was shortest in those with pre-exi ... | 1978 | 211414 |
| molecular genetics of herpes simplex virus: demonstration of regions of obligatory and nonobligatory identity within diploid regions of the genome by sequence replacement and insertion. | the dnas of herpes simplex virus (hsv) 1 and 2 consist of two components, l and s, each composed of unique sequences bracketed by inverted repeats. in this study we have probed the structure of the reiterated regions of the s component in marker rescue experiments involving transfection of cells with mixtures of intact hsv-1 mutant viral dna and individual dna fragments generated by restriction endonuclease digestion of wild-type hsv-1 or hsv-2 dnas. the results were as follows: (i) hsv is diplo ... | 1978 | 211508 |
| optical diffraction analysis of electron microscope images of tubular structures found in cells infected with herpes simplex virus type 2. | optical diffraction analysis was done on the electron micrographs of tubular structures found in cells infected with herpes simplex virus type 2. by this method, the helical arrangement of subunits forming a tubular structure was demonstrated. | 1978 | 212515 |
| suppression of the synthesis of cellular macromolecules by herpes simplex virus. | synthesis of cellular protein was substantially inhibited within 1 h of infection with herpes simplex virus, type 2, strain g (hsv-2). the inhibition also occurred, although no virus-specific protein synthesis was detected, after infection with u.v. irradiated virus and in cytoplasts that had been enucleated before infection. the inhibitory activity could not be distinguished from infectivity by dilution, sedimentation or reaction with gamma-globulin. hsv-2 also suppresssed the synthesis of send ... | 1978 | 212520 |
| collaborative complementation study of temperature-sensitive mutants of herpes simplex virus types 1 and 2. | twenty-three complementation groups of herpes simplex virus type 1 (hsv-1) and 20 of hsv-2 were identified by qualitative and quantitative complementation analysis from among 43 temperature-sensitive (ts) mutants of hsv-1 and 29 ts mutants of hsv-2 which had been isolated independently in 10 laboratories. | 1978 | 212578 |
| severe thrombocytopenia after generalized herpes simplex virus-2 (hsv-2) infection. | our patient, we believe, experienced a primary hsv-2 infection manifested by fever, malaise, and orogenital mucocutaneous lesions. with resolution of systemic manifestations, and at a time of theorized rise in hsv-2 antibody levels, he had thrombocytopenic purpura which persisted for several weeks. an immune disorder secondary to hsv-2 infection similar to that of postrubella infection is postulated. if this theory is correct, severe immune thrombocytopenic purpura is yet one more of the many ob ... | 1978 | 566472 |
| antiviral activity of extracts from marine algae. | extracts of two species of marine algae, constantinea simplex and farlowia mollis, were tested for antiviral activity in tissue culture and in experimental infections of mice. treatment of confluent mouse embryo fibroblast cell monolayers with either compound before viral inoculation was effective in inhibiting the replication of herpes simplex virus type 1 and type 2, vaccinia virus, and vesicular stomatitis virus, but not encephalomyocarditis virus, semliki forest virus, or murine cytomegalovi ... | 1978 | 686707 |
| recurrent herpetic whitlow due to herpes simplex virus type 2. | herpes simplex virus type 2 was demonstrated to be the cause of a recurrent painful and disabling digital infection in a young man. it is possible that this was originally venereally transmitted. with increasing incidence of genital herpes infections, digital herpetic infections may become more common in the nonmedically employed community. | 1978 | 686934 |
| antibody-mediated recovery from subcutaneous herpes simplex virus type 2 infection. | young adult mice infected with 3 x 10(6) plaque-forming units of herpes simplex virus type 2 (hsv-2) died within 9 to 12 days after spread of the virus from the sites of infection to the spinal cord and brain. administration of hsv-2-neutralizing antisera prepared in syngenic mice or in rabbits inhibited spread of the virus from the footpad of infected animals and prevented death. a single intraperitoneal inoculum of antiserum (virus-neutralizing titer of 1:128) was effective in protecting mice ... | 1978 | 689732 |
| application of antisera to interferon in studying oncogenic viruses. | susceptibility of balb/c mice to infection with moloney sarcoma virus (msv) and to herpes simplex virus type 2 (hsv-2) was considerably increased by administration of sheep anti-mouse interferon (anti-if) serum. the regression of the msv-induced tumors was inhibited when the weanling (three-to-four-week-old) mice were injected with the anti-if serum. using the anti-if serum it has been found that the antagonism between hsv-2 and rauscher leukemia virus in the mouse is not mediated by interferon. ... | 1978 | 219809 |
| antibody to herpes simplex virus type 2-induced early antigens and its radioimmunoassay with sera of cervical carcinoma patients. | 1978 | 225600 | |
| new treatment for herpes simplex virus type 2 [ultrasound and zinc, urea, and tannic acid ointment]. part i--male patients. | twenty-three male patients with herpes simplex virus type 2 blisters on the prepuce, glans penis, and penile shaft were divided into two groups. ten patients served as controls, and thirteen patients were treated with ultrasound (1 watt/cm2 for 60 seconds) and herpigon on three consecutive days. results showed a negative virus culture after three days of treatment and significant reduction of recurrent infection in the treated group while the control group experienced recurrent infection within ... | 1978 | 283170 |
| role of t lymphocytes in cellular immune responses during herpes simplex virus infection in humans. | lymphocyte blast transformation and interferon production in mononuclear cell culture prepared on ficoll-hypaque gradients from individuals with herpes simplex virus-i infection were enhanced by a disease recurrence. responses to both herpes simplex virus-2 and phytohemagglutinin were unaltered. transformation to herpes simplex virus-i antigen was adversely affected by depleting either thymus-derived (t) lymphocytes or bone marrow-derived (b) lymphocytes together with monocytes from cultures. th ... | 1978 | 308659 |
| [the importance of herpes simplex virus type 2 (hsv-2) infection pregnancy and the period following birth (author's transl)]. | the type 2 herpes simplex virus is mainly localized in the genital region and is transmitted by sexual intercourse. a distinction must be made between primary and recurrent infection. during pregnancy, infection of the generative tract with hsv-2 can be expected in 0.1% of cases, depending on the patient's social status. it was possible to culture hsv-2 from the cervical secretion in 0.65% of an investigated group of pregnant women. fifty percent of all infections in pregnancy are asymptomatic. ... | 1977 | 341550 |
| disodium phosphonoacetate in cream base as a possible topical treatment for skin lesions of herpes simplex virus in cebus monkeys. | disodium phosphonoacetate (paa) in a cream-ointment base was applied to herpesvirus skin lesions on the genitalia of cebus monkeys. the lesions had been produced by the intradermal injection of herpes simplex virus type 2. concentrations of 0.2, 2, and 5% paa were used. liberal application of paa at concentrations of 2 and 5% proved extremely irritating and produced extensive, severe lesions over the treated area. the 2% paa, when applied carefully to the lesion area, proved less irritating, but ... | 1977 | 411417 |
| chemotherapy of genital herpes simplex virus type 2 infections of female hamsters. | the antiviral activity of 1-beta-d-arabinofuranosylcytosine (ara-c, cytarabine, cytosar), 5-iodo-2'-deoxyuridine (idurd), 9-beta-d-arabinofuranosyladenine (ara-a), and disodium phosphonoacetate (paa) have been compared in herpes simplex virus type 2 (hsv-2)-infected primary rabbit kidney cells and in female hamsters with genital hsv-2 infection. in vitro, ara-c and idurd were more active than ara-a, and paa was least active. in female hamsters with genital hsv-2 infection, intravaginal treatment ... | 1977 | 856023 |
| a rational approach to the development of antiviral chemotherapy: alternative substrates of herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2) thymidine kinase (tk). | 1977 | 212988 | |
| congenital herpes simplex virus, type 2, bilateral endophthalmitis. | a set of dizygotic twins, both born prematurely, developed herpes simplex encephalitis shortly after birth. the second twin had unilateral keratoconjunctivitis and bilateral endophthalmitis and subsequently died from disseminated herpes infection. herpes simplex, type 2, was isolated from conjunctiva of both eyes, cerebrospinal fluid, nasopharynx and trachea. histologic examination of the eyes reveaed bilateral endophthalmitis with a necrotizing retinitis. intranuclear inclusion bodies were demo ... | 1977 | 206999 |
| incidence and distribution of herpes simplex virus types 1 and 2 from genital lesions in college women. | during a 9-month period, 9,772 women were treated at the student health center's gynecology clinic. herpes simplex virus was isolated from 30 of 57 patients clinically diagnosed as suffering from a herpetic or herpetic-like genial infection for a virological incidence rate of 0.31%. using virus plaque diameter in chick embryo cells and heat stability of viral thymidine kinase, 37% of the isolates were classified as herpes simplex virus type 1 and 63% were classified as herpes simplex virus type ... | 1977 | 204741 |
| immune interactions with cells infected with herpes simplex virus: antibodies to radioiodinated surface antigens. | lactoperoxidase-catalyzed radioiodination was used to study reactions between surface antigens and antibodies on bhk-21 cells infected with hsv-1 and hsv-2. isolation of iodinated surface antigens was achieved by indirect immune precipitation of triton x-100 disrupted cells with antisera to hsv and igg. analysis of immune precipitates by polyacrylamide gel electrophoresis (page) revealed at least 10 antigens, ranging in m.w. from 35 x 103 to 160 x 103 daltons. antigens were detectable on cell su ... | 1977 | 187696 |
| [photodynamic inactivation of herpes simplex virus: ii. photodynamic inactivation with proflavine in experimental herpetic keratitis (author's transl)]. | 1977 | 204176 | |
| correlation of herpes simplex virus types 1 and 2 with clinical features of infection. | strains (338) of herpes simplex virus (hsv) were isolated in stockholm during 1965-1974. by immunoelectroosmophoresis it was possible to identify all strains as either hsv type 1 (hsv-1) or 2 (hsv-2). no strains of intermediate antigenic type or with untypable characteristics were found. the antigenic type of hsv was correlated with body site and clinical features of infection. a case of severe, recurrent, abdominal pain in association with hsv-2 infection is described. in one patient with acute ... | 1977 | 188954 |
| lymphocyte stimulation by herpes simplex virus (hsv) antigens. 1. culture conditions, cell fractionation and presentation of hsv antigens. | optimal culture conditions for rabbit lymphocytes were established. inclusion of 2-mercaptoethanol in the medium greatly enhanced responses. lymphocytes from the blood of hsv-immunized rabbits responded specifically in vitro to heat-killed (56 degree, 60 min) or uv-inactivated hsv preparations. a uv dose of 13,392 ergs/mm2 was the most suitable inactivating dose. oral intramucosal injection was the most effective way of generating blood lymphocyte responses to hsv, but animals immunized in trade ... | 1977 | 216529 |
| expression of an early, nonstructural antigen of herpes simplex virus in cell transformed in vitro by herpes simplex virus. | hyperimmune rabbit antiserum to an early, nonstructural herpes simplex virus type 2 (hsv-2)-induced polypeptide (vp143) reacted in immunofluorescence tests with a variety of cell lines transformed by hsv-2. cytoplasmic fluorescence was observed in 10 to 50% of hsv-2-transformed cells, whereas no fluorescence was observed in cells transformed by other oncogenic dna viruses or by a chemical carcinogen. vp143-specific reactivity could be absorbed from anti-vp143 serum with hsv-2-transformed cells b ... | 1977 | 64626 |
| occurrence of genital herpes simplex and cytomegalovirus infections in pregnancy. | a total of 244 pregnant women, 172 in the first and 72 in the third trimester, were screened for genital herpes simplex (hsv) and cytomegalovirus (cmv) infections using virus isolation, cytological examination and serological studies. in addition, immuno-fluorescence staining of exfoliated cervical cells was used for the detection of hsv infections. herpes simplex virus (hsv) could not be isolated and no characteristic hsv altered cells were found in cytological (papa) smears. cytomegalovirus wa ... | 1977 | 67744 |
| detection of herpes simplex virus-related antigens in the nuclei and cytoplasm of biochemically transformed cells with peroxidase/anti-peroxidase immunological staining and indirect immunofluorescence. | herpes simplex virus (hsv)-related antigens have been demonstrated in the nuclei and cytoplasm of human and mouse cells biochemically transformed by ultraviolet light-irradiated hsv. this was accomplished by using peroxidase/anti-peroxidase immunological staining and indirect immunofluorescence with rabbit antisera that had high neutralizing titers against the hsv-specific thymidine kinase activity and virus infectivity. hsv-1 antisera reacted with antigens in cells biochemically transformed by ... | 1977 | 71279 |
| immune responses to vaginal or systemic infection of balb/c mice with herpes simplex virus type 2. | the temporal relationships among the humoral and cellular immune responses were defined in balb/c mice after vaginal or systemic infection with herpes simplex virus type 2 (hsv-2). after vaginal infection, mice showed evidence of clinical vaginitis on days 4 to 6 and hsv-2 replication was detected locally in the vaginal secretions, cervix, vagina, and uterus before the virus subsequently spread to the central nervous system. death from encephalitis occurred between 7 and 10 days after infection. ... | 1977 | 72115 |
| elisa for herpes simplex virus type 2 antibodies. | 1977 | 72945 |